Reviewed, UniProtKB/Swiss-Prot Q62689 (JAK2_RAT)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase JAK2
    EC=2.7.10.2
Alternative name(s):
    Janus kinase 2
      Short name=JAK-2

Gene names

Name:

Jak2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1132 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Tyrosine kinase of the non-receptor type, involved in interleukin-3 and probably interleukin-23 signal transduction. Plays a role in leptin signaling and control of body weight By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with IL23R, SIRPA, SH2B1, SKB1 and STAM2 By similarity.
Subcellular location	Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated By similarity.
Tissue specificity	Highly abundant in brain and spleen, less abundant in skeletal muscle and testis, and detectable in kidney, heart, lung and liver.
Domain	Possesses two phosphotransferase domains. The second one probably contains the catalytic domain By similarity, while the presence of slight differences suggest a different role for domain 1.
Post-translational modification	Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from mutant phenotype. Source: RGD activation of MAPKK activity Inferred from mutant phenotype. Source: RGD axon regeneration Inferred from mutant phenotype. Source: RGD elevation of cytosolic calcium ion concentration Inferred from mutant phenotype. Source: RGD hormone-mediated signaling pathway Inferred from direct assay. Source: RGD induction of apoptosis by oxidative stress Inferred from mutant phenotype. Source: RGD mineralocorticoid receptor signaling pathway Inferred from mutant phenotype. Source: RGD negative regulation of cell-cell adhesion Inferred from mutant phenotype. Source: RGD negative regulation of heart contraction Inferred from mutant phenotype. Source: RGD neuroprotection Inferred from mutant phenotype. Source: RGD platelet-derived growth factor receptor signaling pathway Inferred from mutant phenotype. Source: RGD positive regulation of cell activation Inferred from mutant phenotype. Source: RGD positive regulation of cell differentiation Inferred from mutant phenotype. Source: RGD positive regulation of cell migration Inferred from mutant phenotype. Source: RGD positive regulation of cell proliferation Inferred from mutant phenotype. Source: RGD positive regulation of inflammatory response Inferred from mutant phenotype. Source: RGD positive regulation of insulin secretion Inferred from mutant phenotype. Source: RGD positive regulation of interleukin-1 beta production Inferred from mutant phenotype. Source: RGD positive regulation of nitric oxide biosynthetic process Inferred from mutant phenotype. Source: RGD positive regulation of phosphoprotein phosphatase activity Inferred from mutant phenotype. Source: RGD positive regulation of protein import into nucleus, translocation Inferred from mutant phenotype. Source: RGD positive regulation of transcription factor activity Inferred from mutant phenotype. Source: RGD protein amino acid autophosphorylation Inferred from mutant phenotype. Source: RGD response to hydroperoxide Inferred from mutant phenotype. Source: RGD tyrosine phosphorylation of Stat1 protein Inferred from mutant phenotype. Source: RGD tyrosine phosphorylation of Stat3 protein Inferred from mutant phenotype. Source: RGD
Cellular component	cytoskeleton Inferred from electronic annotation. Source: InterPro cytosol Inferred from direct assay. Source: RGD extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Inferred from direct assay. Source: RGD nuclear matrix Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Janus kinase activity Inferred from mutant phenotype. Source: RGD acetylcholine receptor binding Inferred from physical interaction. Source: RGD growth hormone receptor binding Inferred from physical interaction. Source: UniProtKB insulin receptor substrate binding Inferred from physical interaction. Source: RGD non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC peptide hormone receptor binding Inferred from physical interaction. Source: RGD phosphoinositide 3-kinase binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1132	1132	Tyrosine-protein kinase JAK2		PRO_0000088114
Regions
	Domain	37 – 380	344	FERM
	Domain	401 – 482	82	SH2; atypical
	Domain	545 – 809	265	Protein kinase 1
	Domain	849 – 1126	278	Protein kinase 2
	Nucleotide binding	855 – 863	9	ATP By similarity
Sites
	Active site	976	1	Proton acceptor By similarity
	Binding site	882	1	ATP By similarity
Amino acid modifications
	Modified residue	523	1	Phosphoserine By similarity
	Modified residue	570	1	Phosphotyrosine By similarity
	Modified residue	813	1	Phosphotyrosine By similarity
	Modified residue	1007	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	1008	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q62689-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: C3AEDF2FECE8B95A	FASTA	1,132	130,585
	10 20 30 40 50 60 MGMACLTMTE MEGTSTSPAH QNGDIPGNAN SVKQTEPVLQ VYLYHSLGQA EGDYLKFPNG 70 80 90 100 110 120 EYVAEEICVA ASKACGITPV YHNMFALMSE TERIWYPPNH VFHIDESTRH NILYRIRFYF 130 140 150 160 170 180 PHWYCSGSNR TYRYGVSRGA EAPLLDDFVM SYLFAQWRHD FVHGWIKVPV THETQEECLG 190 200 210 220 230 240 MAVLDMMRIA KEKDQTPLAV YNSISYKTFL PKCVRAKIQD YHILTRKRIR YRFRRFIQQF 250 260 270 280 290 300 SQCKATARNL KLKYLINLET LQSAFYTEQF EVKESARGPS GEEIFATIII TGNGGIQWSR 310 320 330 340 350 360 GKHKESETLT EQDLQLYCDF PDIIDVSIKQ ANQECSTESR VVTVHKQDGK VLEIELSSLK 370 380 390 400 410 420 EALSFVSLID GYYRLTADAH HYLCKEVAPP AVLENIHSNC HGPISMDFAI SKLKKAGNQT 430 440 450 460 470 480 GLYVLRCSPK DFNKYFLTFA VERENVIEYK HCLITKNENG EYNLSGTKRN FSSLKDLLNC 490 500 510 520 530 540 YQMETVRSDS IIFQFTKCCP PKPKDKSNLL VFRTNGVSDV QLSPTLQRHN NVNQMVFHKI 550 560 570 580 590 600 RNEDLIFNES LGQGTFTKIF KGVRREVGDY GQLHETEVLL KVLDKAHRNY SESFFEAASM 610 620 630 640 650 660 MSQLSHKHLV LNYGVCVCGE ENILVQEFVK FGSLDTYLKK NKNSINILWK LGVAKQLAWA 670 680 690 700 710 720 MHFLEEKSLI HGNVCAKNIL LIREEDRKTG NPPFIKLSDP GISITVLPKD ILQERIPWVP 730 740 750 760 770 780 PECIENPKNL TLATDKWSFG TTLWEICSGG DKPLSALDSQ RKLQFYEDKH QLPAPKWTEL 790 800 810 820 830 840 ANLINTCMDY EPDFRPAFRA VIRDLNSLFT PDYELLTEND MLPNMRIGAL GFSGAFEDRD 850 860 870 880 890 900 PTQFEERHLK FLQQLGKGNF GSVEMCRYDP LQDNTGEVVA VKKLQHSTEE HLRDFEREIE 910 920 930 940 950 960 ILKSLQHDNI VKYKGVCYSA GRRNLRLIME YLPYGSLRDY LQKHKERIDH KKLLQYTSQI 970 980 990 1000 1010 1020 CKGMEYLGTK RYIHRDLATR NILVENENRV KIGDFGLTKV LPQDKEYYKV KEPGESPIFW 1030 1040 1050 1060 1070 1080 YAPESLTESK FSVASDVWSF GVVLYELFTY IEKSKSPPVE FMRMIGNDKQ GQMIVFHLIE 1090 1100 1110 1120 1130 LLKNNGRLPR PEGCPDEIYV IMTECWNNNV NQRPSFRDLS LRVDQIRDSM AA

References

[1]	"Cloning of the gene encoding rat JAK2, a protein tyrosine kinase." Duhe R.J., Rui H., Greenwood J.D., Garvey K., Farrar W.L. Gene 158:281-285(1995) [PubMed: 7607555] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U13396 mRNA. Translation: AAA79911.1.
IPI	IPI00207182.
PIR	JC4127.
RefSeq	NP_113702.1.
UniGene	Rn.18909
3D structure databases
HSSP	HSSP built from PDB template 1FGK based on UniProtKB P11362.
SMR	Q62689. Positions 840-1132.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:491N.
STRING	Q62689.
PTM databases
PhosphoSite	Q62689.
Genome annotation databases
Ensembl	ENSRNOT00000021217; ENSRNOP00000021218; ENSRNOG00000015547; Rattus norvegicus. [Genome view]
GeneID	24514.
KEGG	rno:24514.
UCSC	NM_031514. rat.
Organism-specific databases
CTD	24514.
RGD	2939. Jak2.
Phylogenomic databases
HOVERGEN	Q62689.
Enzyme and pathway databases
BRENDA	2.7.10.2. 248.
Gene expression databases
ArrayExpress	Q62689.
Genevestigator	Q62689.
GermOnline	ENSRNOG00000015547. Rattus norvegicus.
Family and domain databases
InterPro	IPR019749. Band_41_domain. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR009127. JAK. IPR009129. JAK2. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR016251. TyrPK_Jak. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 2 hits. PF00017. SH2. 1 hit. [Graphical view]
PIRSF	PIRSF000636. TyrPK_Jak. 1 hit.
PRINTS	PR01823. JANUSKINASE. PR01825. JANUSKINASE2. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 2 hits. PD000093. SH2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00295. B41. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 2 hits. [Graphical view]
PROSITE	PS00660. FERM_1. False negative. PS00661. FERM_2. False negative. PS50057. FERM_3. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 2 hits. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603541.

Entry information

Entry name

JAK2_RAT

Accession

Primary (citable) accession number: Q62689

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	October 13, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)