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Protein

Oxysterols receptor LXR-alpha

Gene

Nr1h3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nuclear receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Exhibits a ligand-dependent transcriptional activation activity (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi93 – 16876Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri96 – 11621NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri132 – 15625NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • retinoid X receptor binding Source: RGD
  • sequence-specific DNA binding Source: RGD
  • steroid hormone receptor activity Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterols receptor LXR-alpha
Alternative name(s):
Liver X receptor alpha
Nuclear receptor subfamily 1 group H member 3
RLD-1
Gene namesi
Name:Nr1h3
Synonyms:Lxra
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61909. Nr1h3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • cytoplasm Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Oxysterols receptor LXR-alphaPRO_0000053537Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62685.
PRIDEiQ62685.

PTM databases

PhosphoSiteiQ62685.

Expressioni

Tissue specificityi

In adults it is expressed in spleen, pituitary, lung, liver, and fat. Weaker expression is observed in several other tissues.

Interactioni

Subunit structurei

Heterodimer of LXRA and RXR. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with SIRT1 and this interaction is inhibited by CCAR2.By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • retinoid X receptor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018154.

Structurei

3D structure databases

ProteinModelPortaliQ62685.
SMRiQ62685. Positions 94-179, 203-445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9494Transactivation AF-1; required for ligand-independent transactivation functionBy similarityAdd
BLAST
Regioni203 – 445243Transactivation AF-2; required for ligand-dependent transactivation function; mediates interaction with CCAR2By similarityAdd
BLAST
Regioni213 – 432220Ligand-bindingSequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri96 – 11621NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri132 – 15625NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
HOGENOMiHOG000220845.
HOVERGENiHBG108655.
InParanoidiQ62685.
PhylomeDBiQ62685.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR023257. Liver_X_rcpt.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR02034. LIVERXRECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLWLEAAVP DVSPDSATEL WKTEPQDAGD QGGNTCILRE EARMPQSTGG
60 70 80 90 100
ALRIGLESSE PTALLPRAET LPEPTELRPQ KRKKGPAPKM LGNELCSVCG
110 120 130 140 150
DKASAFHYNV LSCEGCKGFF RRSVIKGARY ICHSGGHCPM DTYMRRKCQE
160 170 180 190 200
CRLRKCRHAG MREECVLSEE QIRLKKLKRQ EEEQAQATSV SPRVSSPPQV
210 220 230 240 250
LPQLSPEQLG MIEKLVAAQQ QCNRRSFSDR LRVTPWPIAP DPQSREARQQ
260 270 280 290 300
RFAHFTELAI VSVQEIVDFA KQLPGFLQLS REDQIALLKT SAIEVMLLET
310 320 330 340 350
SRRYNPGSES ITFLKDFSYN REDFAKAGLQ VEFINPIFEF SRSMNELQLN
360 370 380 390 400
DAEFALLIAI SIFSADRPNV QDQLQVERLQ HTYVEALHAY VSINHPHDRL
410 420 430 440
MFPRMLMKLV SLRTLSSVHS EQVFALRLQD KKLPPLLSEI WDVHE
Length:445
Mass (Da):50,555
Last modified:November 1, 1996 - v1
Checksum:i131A84AB142AEA82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11685 mRNA. Translation: AAA53633.1.
PIRiA56043.
UniGeneiRn.11209.

Genome annotation databases

UCSCiRGD:61909. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11685 mRNA. Translation: AAA53633.1.
PIRiA56043.
UniGeneiRn.11209.

3D structure databases

ProteinModelPortaliQ62685.
SMRiQ62685. Positions 94-179, 203-445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018154.

PTM databases

PhosphoSiteiQ62685.

Proteomic databases

PaxDbiQ62685.
PRIDEiQ62685.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:61909. rat.

Organism-specific databases

RGDi61909. Nr1h3.

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
HOGENOMiHOG000220845.
HOVERGENiHBG108655.
InParanoidiQ62685.
PhylomeDBiQ62685.

Miscellaneous databases

PROiQ62685.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR023257. Liver_X_rcpt.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR02034. LIVERXRECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel orphan receptor specific for a subset of thyroid hormone-responsive elements and its interaction with the retinoid/thyroid hormone receptor subfamily."
    Apfel R.H., Benbrook D., Lernhardt E., Ortiz M.A., Salbert G., Pfahl M.
    Mol. Cell. Biol. 14:7025-7035(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-191, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNR1H3_RAT
AccessioniPrimary (citable) accession number: Q62685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.