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Q62674

- S28A1_RAT

UniProt

Q62674 - S28A1_RAT

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Protein
Sodium/nucleoside cotransporter 1
Gene
Slc28a1, Cnt1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). May be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS).

GO - Molecular functioni

  1. nucleoside binding Source: InterPro
  2. pyrimidine nucleobase transmembrane transporter activity Source: RGD
  3. pyrimidine- and adenine-specific:sodium symporter activity Source: RGD

GO - Biological processi

  1. purine nucleobase transport Source: GOC
  2. pyrimidine nucleobase transport Source: RGD
  3. pyrimidine-containing compound transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi2.A.41.2.2. the concentrative nucleoside transporter (cnt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/nucleoside cotransporter 1
Alternative name(s):
Concentrative nucleoside transporter 1
Short name:
CNT 1
Na(+)/nucleoside cotransporter 1
Sodium-coupled nucleoside transporter 1
Solute carrier family 28 member 1
Gene namesi
Name:Slc28a1
Synonyms:Cnt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621223. Slc28a1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 8080Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei81 – 10424Helical; Reviewed prediction
Add
BLAST
Topological domaini105 – 1095Extracellular Reviewed prediction
Transmembranei110 – 12819Helical; Reviewed prediction
Add
BLAST
Topological domaini129 – 14719Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei148 – 16720Helical; Reviewed prediction
Add
BLAST
Topological domaini168 – 17811Extracellular Reviewed prediction
Add
BLAST
Transmembranei179 – 19517Helical; Reviewed prediction
Add
BLAST
Topological domaini196 – 2016Cytoplasmic Reviewed prediction
Transmembranei202 – 22221Helical; Reviewed prediction
Add
BLAST
Topological domaini223 – 26139Extracellular Reviewed prediction
Add
BLAST
Transmembranei262 – 28322Helical; Reviewed prediction
Add
BLAST
Topological domaini284 – 29411Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei295 – 31824Helical; Reviewed prediction
Add
BLAST
Topological domaini319 – 33719Extracellular Reviewed prediction
Add
BLAST
Transmembranei338 – 36023Helical; Reviewed prediction
Add
BLAST
Topological domaini361 – 3666Cytoplasmic Reviewed prediction
Transmembranei367 – 38620Helical; Reviewed prediction
Add
BLAST
Topological domaini387 – 42337Extracellular Reviewed prediction
Add
BLAST
Transmembranei424 – 44623Helical; Reviewed prediction
Add
BLAST
Topological domaini447 – 45711Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei458 – 47922Helical; Reviewed prediction
Add
BLAST
Topological domaini480 – 53455Extracellular Reviewed prediction
Add
BLAST
Transmembranei535 – 55824Helical; Reviewed prediction
Add
BLAST
Topological domaini559 – 56911Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei570 – 59223Helical; Reviewed prediction
Add
BLAST
Topological domaini593 – 64856Extracellular Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 648648Sodium/nucleoside cotransporter 1
PRO_0000070449Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi605 – 6051N-linked (GlcNAc...)1 Publication
Glycosylationi643 – 6431N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ62674.

Expressioni

Tissue specificityi

Expressed predominantly in the brush-border membranes of the polarized epithelial cells of jejunum and renal cortical tubules and in the bile canalicular membranes of liver parenchymal cells.1 Publication

Gene expression databases

GenevestigatoriQ62674.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025621.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1972.
HOGENOMiHOG000267658.
HOVERGENiHBG054073.
InParanoidiQ62674.
KOiK11536.
PhylomeDBiQ62674.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Nucleoside_recog_Gate.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62674-1 [UniParc]FASTAAdd to Basket

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MADNTQRQRE SISLTPMAHG LENMGAEFLE SMEEGRLPHS HSSLPEGEGG    50
LNKAERKAFS RWRSLQPTVQ ARSFCREHRQ LFGWICKGLL STACLGFLMV 100
ACLLDLQRAL ALLIITCVVL VFLAYDLLKR LLGSKLRRCV KFQGHSCLSL 150
WLKRGLALAA GVGLILWLSL DTAQRPEQLV SFAGICVFLV LLFAGSKHHR 200
AVSWRAVSWG LGLQFVLGLF VIRTEPGFIA FQWLGDQIQV FLSYTEAGSS 250
FVFGEALVKD VFAFQVLPII IFFSCVMSVL YYLGLMQWVI LKIAWLMQVT 300
MGTSATETLS VAGNIFVSQT EAPLLIRPYL ADMTLSEVHV VMTGGYATIA 350
GSLLGAYISF GIDAASLIAA SVMAAPCALA LSKLVYPEVE ESKFRSENGV 400
KLTYGDAQNL LEAASAGAAI SVKVVANIAA NLIAFLAVLA FVNAALSWLG 450
DMVDIQGLSF QLICSYVLRP VAFLMGVAWE DCPVVAELLG IKFFLNEFVA 500
YQELSQYKQR RLAGAEEWLG DKKQWISVRA EILTTYALCG FANFSSIGIM 550
LGGLTSLVPQ RRSDFSQIVL RALITGAFVS LLNACVAGIL YVPRGVEVDC 600
VSLLNQTVSS SSFEVYLCCR QVFQSTSSEF SQVALDNCCR FYNHTVCT 648
Length:648
Mass (Da):71,001
Last modified:November 1, 1996 - v1
Checksum:i7618B4436DAED1A8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10279 mRNA. Translation: AAB03626.1.
PIRiA54892.
RefSeqiNP_446315.1. NM_053863.1.
UniGeneiRn.10517.

Genome annotation databases

GeneIDi116642.
KEGGirno:116642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U10279 mRNA. Translation: AAB03626.1 .
PIRi A54892.
RefSeqi NP_446315.1. NM_053863.1.
UniGenei Rn.10517.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000025621.

Chemistry

ChEMBLi CHEMBL1287615.

Protein family/group databases

TCDBi 2.A.41.2.2. the concentrative nucleoside transporter (cnt) family.

Proteomic databases

PRIDEi Q62674.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 116642.
KEGGi rno:116642.

Organism-specific databases

CTDi 9154.
RGDi 621223. Slc28a1.

Phylogenomic databases

eggNOGi COG1972.
HOGENOMi HOG000267658.
HOVERGENi HBG054073.
InParanoidi Q62674.
KOi K11536.
PhylomeDBi Q62674.

Miscellaneous databases

NextBioi 619371.
PROi Q62674.

Gene expression databases

Genevestigatori Q62674.

Family and domain databases

InterProi IPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Nucleoside_recog_Gate.
[Graphical view ]
PANTHERi PTHR10590. PTHR10590. 1 hit.
Pfami PF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00804. nupC. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and functional expression of a complementary DNA encoding a mammalian nucleoside transport protein."
    Huang Q.-Q., Yao S.Y.M., Ritzel M.W.L., Paterson A.R.P., Cass C.E., Young J.D.
    J. Biol. Chem. 269:17757-17760(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Jejunum.
  2. "Subcellular distribution and membrane topology of the mammalian concentrative Na+-nucleoside cotransporter rCNT1."
    Hamilton S.R., Yao S.Y., Ingram J.C., Hadden D.A., Ritzel M.W., Gallagher M.P., Henderson P.J., Cass C.E., Young J.D., Baldwin S.A.
    J. Biol. Chem. 276:27981-27988(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-605 AND ASN-643.

Entry informationi

Entry nameiS28A1_RAT
AccessioniPrimary (citable) accession number: Q62674
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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