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Protein

Sodium/nucleoside cotransporter 1

Gene

Slc28a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). May be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS).

GO - Molecular functioni

  • pyrimidine- and adenine-specific:sodium symporter activity Source: RGD
  • pyrimidine nucleobase transmembrane transporter activity Source: RGD

GO - Biological processi

  • pyrimidine nucleobase transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi2.A.41.2.2. the concentrative nucleoside transporter (cnt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/nucleoside cotransporter 1
Alternative name(s):
Concentrative nucleoside transporter 1
Short name:
CNT 1
Na(+)/nucleoside cotransporter 1
Sodium-coupled nucleoside transporter 1
Solute carrier family 28 member 1
Gene namesi
Name:Slc28a1
Synonyms:Cnt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621223. Slc28a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 80CytoplasmicSequence analysisAdd BLAST80
Transmembranei81 – 104HelicalSequence analysisAdd BLAST24
Topological domaini105 – 109ExtracellularSequence analysis5
Transmembranei110 – 128HelicalSequence analysisAdd BLAST19
Topological domaini129 – 147CytoplasmicSequence analysisAdd BLAST19
Transmembranei148 – 167HelicalSequence analysisAdd BLAST20
Topological domaini168 – 178ExtracellularSequence analysisAdd BLAST11
Transmembranei179 – 195HelicalSequence analysisAdd BLAST17
Topological domaini196 – 201CytoplasmicSequence analysis6
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
Topological domaini223 – 261ExtracellularSequence analysisAdd BLAST39
Transmembranei262 – 283HelicalSequence analysisAdd BLAST22
Topological domaini284 – 294CytoplasmicSequence analysisAdd BLAST11
Transmembranei295 – 318HelicalSequence analysisAdd BLAST24
Topological domaini319 – 337ExtracellularSequence analysisAdd BLAST19
Transmembranei338 – 360HelicalSequence analysisAdd BLAST23
Topological domaini361 – 366CytoplasmicSequence analysis6
Transmembranei367 – 386HelicalSequence analysisAdd BLAST20
Topological domaini387 – 423ExtracellularSequence analysisAdd BLAST37
Transmembranei424 – 446HelicalSequence analysisAdd BLAST23
Topological domaini447 – 457CytoplasmicSequence analysisAdd BLAST11
Transmembranei458 – 479HelicalSequence analysisAdd BLAST22
Topological domaini480 – 534ExtracellularSequence analysisAdd BLAST55
Transmembranei535 – 558HelicalSequence analysisAdd BLAST24
Topological domaini559 – 569CytoplasmicSequence analysisAdd BLAST11
Transmembranei570 – 592HelicalSequence analysisAdd BLAST23
Topological domaini593 – 648ExtracellularSequence analysisAdd BLAST56

GO - Cellular componenti

  • apical plasma membrane Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1287615.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000704491 – 648Sodium/nucleoside cotransporter 1Add BLAST648

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi605N-linked (GlcNAc...)1 Publication1
Glycosylationi643N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ62674.
PRIDEiQ62674.

Expressioni

Tissue specificityi

Expressed predominantly in the brush-border membranes of the polarized epithelial cells of jejunum and renal cortical tubules and in the bile canalicular membranes of liver parenchymal cells.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025621.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3747. Eukaryota.
COG1972. LUCA.
HOGENOMiHOG000267658.
HOVERGENiHBG054073.
InParanoidiQ62674.
KOiK11536.
PhylomeDBiQ62674.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Gate_dom.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADNTQRQRE SISLTPMAHG LENMGAEFLE SMEEGRLPHS HSSLPEGEGG
60 70 80 90 100
LNKAERKAFS RWRSLQPTVQ ARSFCREHRQ LFGWICKGLL STACLGFLMV
110 120 130 140 150
ACLLDLQRAL ALLIITCVVL VFLAYDLLKR LLGSKLRRCV KFQGHSCLSL
160 170 180 190 200
WLKRGLALAA GVGLILWLSL DTAQRPEQLV SFAGICVFLV LLFAGSKHHR
210 220 230 240 250
AVSWRAVSWG LGLQFVLGLF VIRTEPGFIA FQWLGDQIQV FLSYTEAGSS
260 270 280 290 300
FVFGEALVKD VFAFQVLPII IFFSCVMSVL YYLGLMQWVI LKIAWLMQVT
310 320 330 340 350
MGTSATETLS VAGNIFVSQT EAPLLIRPYL ADMTLSEVHV VMTGGYATIA
360 370 380 390 400
GSLLGAYISF GIDAASLIAA SVMAAPCALA LSKLVYPEVE ESKFRSENGV
410 420 430 440 450
KLTYGDAQNL LEAASAGAAI SVKVVANIAA NLIAFLAVLA FVNAALSWLG
460 470 480 490 500
DMVDIQGLSF QLICSYVLRP VAFLMGVAWE DCPVVAELLG IKFFLNEFVA
510 520 530 540 550
YQELSQYKQR RLAGAEEWLG DKKQWISVRA EILTTYALCG FANFSSIGIM
560 570 580 590 600
LGGLTSLVPQ RRSDFSQIVL RALITGAFVS LLNACVAGIL YVPRGVEVDC
610 620 630 640
VSLLNQTVSS SSFEVYLCCR QVFQSTSSEF SQVALDNCCR FYNHTVCT
Length:648
Mass (Da):71,001
Last modified:November 1, 1996 - v1
Checksum:i7618B4436DAED1A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10279 mRNA. Translation: AAB03626.1.
PIRiA54892.
RefSeqiNP_446315.1. NM_053863.1.
UniGeneiRn.10517.

Genome annotation databases

GeneIDi116642.
KEGGirno:116642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10279 mRNA. Translation: AAB03626.1.
PIRiA54892.
RefSeqiNP_446315.1. NM_053863.1.
UniGeneiRn.10517.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025621.

Chemistry databases

ChEMBLiCHEMBL1287615.

Protein family/group databases

TCDBi2.A.41.2.2. the concentrative nucleoside transporter (cnt) family.

Proteomic databases

PaxDbiQ62674.
PRIDEiQ62674.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi116642.
KEGGirno:116642.

Organism-specific databases

CTDi9154.
RGDi621223. Slc28a1.

Phylogenomic databases

eggNOGiKOG3747. Eukaryota.
COG1972. LUCA.
HOGENOMiHOG000267658.
HOVERGENiHBG054073.
InParanoidiQ62674.
KOiK11536.
PhylomeDBiQ62674.

Miscellaneous databases

PROiQ62674.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Gate_dom.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS28A1_RAT
AccessioniPrimary (citable) accession number: Q62674
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.