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Q62674

- S28A1_RAT

UniProt

Q62674 - S28A1_RAT

Protein

Sodium/nucleoside cotransporter 1

Gene

Slc28a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). May be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS).

    GO - Molecular functioni

    1. nucleoside binding Source: InterPro
    2. pyrimidine- and adenine-specific:sodium symporter activity Source: RGD
    3. pyrimidine nucleobase transmembrane transporter activity Source: RGD

    GO - Biological processi

    1. purine nucleobase transport Source: GOC
    2. pyrimidine-containing compound transmembrane transport Source: GOC
    3. pyrimidine nucleobase transport Source: RGD

    Keywords - Biological processi

    Transport

    Protein family/group databases

    TCDBi2.A.41.2.2. the concentrative nucleoside transporter (cnt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/nucleoside cotransporter 1
    Alternative name(s):
    Concentrative nucleoside transporter 1
    Short name:
    CNT 1
    Na(+)/nucleoside cotransporter 1
    Sodium-coupled nucleoside transporter 1
    Solute carrier family 28 member 1
    Gene namesi
    Name:Slc28a1
    Synonyms:Cnt1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621223. Slc28a1.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. apical plasma membrane Source: RGD
    2. integral component of membrane Source: UniProtKB-KW
    3. membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 648648Sodium/nucleoside cotransporter 1PRO_0000070449Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi605 – 6051N-linked (GlcNAc...)1 Publication
    Glycosylationi643 – 6431N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ62674.

    Expressioni

    Tissue specificityi

    Expressed predominantly in the brush-border membranes of the polarized epithelial cells of jejunum and renal cortical tubules and in the bile canalicular membranes of liver parenchymal cells.1 Publication

    Gene expression databases

    GenevestigatoriQ62674.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025621.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 8080CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini105 – 1095ExtracellularSequence Analysis
    Topological domaini129 – 14719CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini168 – 17811ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini196 – 2016CytoplasmicSequence Analysis
    Topological domaini223 – 26139ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini284 – 29411CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini319 – 33719ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini361 – 3666CytoplasmicSequence Analysis
    Topological domaini387 – 42337ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini447 – 45711CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini480 – 53455ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini559 – 56911CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini593 – 64856ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei81 – 10424HelicalSequence AnalysisAdd
    BLAST
    Transmembranei110 – 12819HelicalSequence AnalysisAdd
    BLAST
    Transmembranei148 – 16720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei179 – 19517HelicalSequence AnalysisAdd
    BLAST
    Transmembranei202 – 22221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei262 – 28322HelicalSequence AnalysisAdd
    BLAST
    Transmembranei295 – 31824HelicalSequence AnalysisAdd
    BLAST
    Transmembranei338 – 36023HelicalSequence AnalysisAdd
    BLAST
    Transmembranei367 – 38620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei424 – 44623HelicalSequence AnalysisAdd
    BLAST
    Transmembranei458 – 47922HelicalSequence AnalysisAdd
    BLAST
    Transmembranei535 – 55824HelicalSequence AnalysisAdd
    BLAST
    Transmembranei570 – 59223HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1972.
    HOGENOMiHOG000267658.
    HOVERGENiHBG054073.
    InParanoidiQ62674.
    KOiK11536.
    PhylomeDBiQ62674.

    Family and domain databases

    InterProiIPR008276. C_nuclsd_transpt.
    IPR018270. C_nuclsd_transpt_met_bac.
    IPR011657. CNT_C_dom.
    IPR002668. CNT_N_dom.
    IPR011642. Nucleoside_recog_Gate.
    [Graphical view]
    PANTHERiPTHR10590. PTHR10590. 1 hit.
    PfamiPF07670. Gate. 1 hit.
    PF07662. Nucleos_tra2_C. 1 hit.
    PF01773. Nucleos_tra2_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00804. nupC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q62674-1 [UniParc]FASTAAdd to Basket

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    MADNTQRQRE SISLTPMAHG LENMGAEFLE SMEEGRLPHS HSSLPEGEGG    50
    LNKAERKAFS RWRSLQPTVQ ARSFCREHRQ LFGWICKGLL STACLGFLMV 100
    ACLLDLQRAL ALLIITCVVL VFLAYDLLKR LLGSKLRRCV KFQGHSCLSL 150
    WLKRGLALAA GVGLILWLSL DTAQRPEQLV SFAGICVFLV LLFAGSKHHR 200
    AVSWRAVSWG LGLQFVLGLF VIRTEPGFIA FQWLGDQIQV FLSYTEAGSS 250
    FVFGEALVKD VFAFQVLPII IFFSCVMSVL YYLGLMQWVI LKIAWLMQVT 300
    MGTSATETLS VAGNIFVSQT EAPLLIRPYL ADMTLSEVHV VMTGGYATIA 350
    GSLLGAYISF GIDAASLIAA SVMAAPCALA LSKLVYPEVE ESKFRSENGV 400
    KLTYGDAQNL LEAASAGAAI SVKVVANIAA NLIAFLAVLA FVNAALSWLG 450
    DMVDIQGLSF QLICSYVLRP VAFLMGVAWE DCPVVAELLG IKFFLNEFVA 500
    YQELSQYKQR RLAGAEEWLG DKKQWISVRA EILTTYALCG FANFSSIGIM 550
    LGGLTSLVPQ RRSDFSQIVL RALITGAFVS LLNACVAGIL YVPRGVEVDC 600
    VSLLNQTVSS SSFEVYLCCR QVFQSTSSEF SQVALDNCCR FYNHTVCT 648
    Length:648
    Mass (Da):71,001
    Last modified:November 1, 1996 - v1
    Checksum:i7618B4436DAED1A8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10279 mRNA. Translation: AAB03626.1.
    PIRiA54892.
    RefSeqiNP_446315.1. NM_053863.1.
    UniGeneiRn.10517.

    Genome annotation databases

    GeneIDi116642.
    KEGGirno:116642.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10279 mRNA. Translation: AAB03626.1 .
    PIRi A54892.
    RefSeqi NP_446315.1. NM_053863.1.
    UniGenei Rn.10517.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025621.

    Chemistry

    ChEMBLi CHEMBL1287615.

    Protein family/group databases

    TCDBi 2.A.41.2.2. the concentrative nucleoside transporter (cnt) family.

    Proteomic databases

    PRIDEi Q62674.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 116642.
    KEGGi rno:116642.

    Organism-specific databases

    CTDi 9154.
    RGDi 621223. Slc28a1.

    Phylogenomic databases

    eggNOGi COG1972.
    HOGENOMi HOG000267658.
    HOVERGENi HBG054073.
    InParanoidi Q62674.
    KOi K11536.
    PhylomeDBi Q62674.

    Miscellaneous databases

    NextBioi 619371.
    PROi Q62674.

    Gene expression databases

    Genevestigatori Q62674.

    Family and domain databases

    InterProi IPR008276. C_nuclsd_transpt.
    IPR018270. C_nuclsd_transpt_met_bac.
    IPR011657. CNT_C_dom.
    IPR002668. CNT_N_dom.
    IPR011642. Nucleoside_recog_Gate.
    [Graphical view ]
    PANTHERi PTHR10590. PTHR10590. 1 hit.
    Pfami PF07670. Gate. 1 hit.
    PF07662. Nucleos_tra2_C. 1 hit.
    PF01773. Nucleos_tra2_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00804. nupC. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional expression of a complementary DNA encoding a mammalian nucleoside transport protein."
      Huang Q.-Q., Yao S.Y.M., Ritzel M.W.L., Paterson A.R.P., Cass C.E., Young J.D.
      J. Biol. Chem. 269:17757-17760(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Jejunum.
    2. "Subcellular distribution and membrane topology of the mammalian concentrative Na+-nucleoside cotransporter rCNT1."
      Hamilton S.R., Yao S.Y., Ingram J.C., Hadden D.A., Ritzel M.W., Gallagher M.P., Henderson P.J., Cass C.E., Young J.D., Baldwin S.A.
      J. Biol. Chem. 276:27981-27988(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-605 AND ASN-643.

    Entry informationi

    Entry nameiS28A1_RAT
    AccessioniPrimary (citable) accession number: Q62674
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3