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Q62674 (S28A1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sodium/nucleoside cotransporter 1
Alternative name(s):
Concentrative nucleoside transporter 1
Short name=CNT 1
Na(+)/nucleoside cotransporter 1
Sodium-coupled nucleoside transporter 1
Solute carrier family 28 member 1
Gene names
Name:Slc28a1
Synonyms:Cnt1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length648 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). May be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS).

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.2.

Tissue specificity

Expressed predominantly in the brush-border membranes of the polarized epithelial cells of jejunum and renal cortical tubules and in the bile canalicular membranes of liver parenchymal cells. Ref.2

Sequence similarities

Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 648648Sodium/nucleoside cotransporter 1
PRO_0000070449

Regions

Topological domain1 – 8080Cytoplasmic Potential
Transmembrane81 – 10424Helical; Potential
Topological domain105 – 1095Extracellular Potential
Transmembrane110 – 12819Helical; Potential
Topological domain129 – 14719Cytoplasmic Potential
Transmembrane148 – 16720Helical; Potential
Topological domain168 – 17811Extracellular Potential
Transmembrane179 – 19517Helical; Potential
Topological domain196 – 2016Cytoplasmic Potential
Transmembrane202 – 22221Helical; Potential
Topological domain223 – 26139Extracellular Potential
Transmembrane262 – 28322Helical; Potential
Topological domain284 – 29411Cytoplasmic Potential
Transmembrane295 – 31824Helical; Potential
Topological domain319 – 33719Extracellular Potential
Transmembrane338 – 36023Helical; Potential
Topological domain361 – 3666Cytoplasmic Potential
Transmembrane367 – 38620Helical; Potential
Topological domain387 – 42337Extracellular Potential
Transmembrane424 – 44623Helical; Potential
Topological domain447 – 45711Cytoplasmic Potential
Transmembrane458 – 47922Helical; Potential
Topological domain480 – 53455Extracellular Potential
Transmembrane535 – 55824Helical; Potential
Topological domain559 – 56911Cytoplasmic Potential
Transmembrane570 – 59223Helical; Potential
Topological domain593 – 64856Extracellular Potential

Amino acid modifications

Glycosylation6051N-linked (GlcNAc...) Ref.2
Glycosylation6431N-linked (GlcNAc...) Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q62674 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7618B4436DAED1A8

FASTA64871,001
        10         20         30         40         50         60 
MADNTQRQRE SISLTPMAHG LENMGAEFLE SMEEGRLPHS HSSLPEGEGG LNKAERKAFS 

        70         80         90        100        110        120 
RWRSLQPTVQ ARSFCREHRQ LFGWICKGLL STACLGFLMV ACLLDLQRAL ALLIITCVVL 

       130        140        150        160        170        180 
VFLAYDLLKR LLGSKLRRCV KFQGHSCLSL WLKRGLALAA GVGLILWLSL DTAQRPEQLV 

       190        200        210        220        230        240 
SFAGICVFLV LLFAGSKHHR AVSWRAVSWG LGLQFVLGLF VIRTEPGFIA FQWLGDQIQV 

       250        260        270        280        290        300 
FLSYTEAGSS FVFGEALVKD VFAFQVLPII IFFSCVMSVL YYLGLMQWVI LKIAWLMQVT 

       310        320        330        340        350        360 
MGTSATETLS VAGNIFVSQT EAPLLIRPYL ADMTLSEVHV VMTGGYATIA GSLLGAYISF 

       370        380        390        400        410        420 
GIDAASLIAA SVMAAPCALA LSKLVYPEVE ESKFRSENGV KLTYGDAQNL LEAASAGAAI 

       430        440        450        460        470        480 
SVKVVANIAA NLIAFLAVLA FVNAALSWLG DMVDIQGLSF QLICSYVLRP VAFLMGVAWE 

       490        500        510        520        530        540 
DCPVVAELLG IKFFLNEFVA YQELSQYKQR RLAGAEEWLG DKKQWISVRA EILTTYALCG 

       550        560        570        580        590        600 
FANFSSIGIM LGGLTSLVPQ RRSDFSQIVL RALITGAFVS LLNACVAGIL YVPRGVEVDC 

       610        620        630        640 
VSLLNQTVSS SSFEVYLCCR QVFQSTSSEF SQVALDNCCR FYNHTVCT

« Hide

References

[1]"Cloning and functional expression of a complementary DNA encoding a mammalian nucleoside transport protein."
Huang Q.-Q., Yao S.Y.M., Ritzel M.W.L., Paterson A.R.P., Cass C.E., Young J.D.
J. Biol. Chem. 269:17757-17760(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Jejunum.
[2]"Subcellular distribution and membrane topology of the mammalian concentrative Na+-nucleoside cotransporter rCNT1."
Hamilton S.R., Yao S.Y., Ingram J.C., Hadden D.A., Ritzel M.W., Gallagher M.P., Henderson P.J., Cass C.E., Young J.D., Baldwin S.A.
J. Biol. Chem. 276:27981-27988(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION AT ASN-605 AND ASN-643.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U10279 mRNA. Translation: AAB03626.1.
IPIIPI00207160.
PIRA54892.
RefSeqNP_446315.1. NM_053863.1.
UniGeneRn.10517.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000025621.

Protein family/group databases

TCDB2.A.41.2.2. concentrative nucleoside transporter (CNT) family.

Proteomic databases

PRIDEQ62674.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID116642.
KEGGrno:116642.

Organism-specific databases

CTD9154.
RGD621223. Slc28a1.

Phylogenomic databases

eggNOGCOG1972.
HOGENOMHOG000267658.
HOVERGENHBG054073.
InParanoidQ62674.
KOK11536.
OrthoDBEOG4V170B.

Gene expression databases

ArrayExpressQ62674.
GenevestigatorQ62674.
GermOnlineENSRNOG00000018940. Rattus norvegicus.

Family and domain databases

InterProIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. Nucleos_tra2_C.
IPR011642. Nucleoside_recog_Gate.
IPR002668. Nuclsd_transpt2.
[Graphical view]
PANTHERPTHR10590. PTHR10590. 1 hit.
PfamPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00804. nupC. 1 hit.
ProtoNetSearch...

Other

ChEMBLCHEMBL1287615.
NextBio619371.

Entry information

Entry nameS28A1_RAT
AccessionPrimary (citable) accession number: Q62674
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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