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Q62668

- UT2_RAT

UniProt

Q62668 - UT2_RAT

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Protein

Urea transporter 2

Gene

Slc14a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. Vasopressin regulates urea transport by increasing isoform 1 accumulation in the plasma membrane and/or phosphorylation of isoform 1.4 Publications

GO - Molecular functioni

  1. urea transmembrane transporter activity Source: RGD

GO - Biological processi

  1. response to water deprivation Source: RGD
  2. urea transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi1.A.28.1.1. the urea transporter (ut) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Urea transporter 2
Alternative name(s):
Solute carrier family 14 member 2
Urea transporter, kidney
Gene namesi
Name:Slc14a2
Synonyms:Ut2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3689. Slc14a2.

Subcellular locationi

Apical cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Urea transporter 2PRO_0000065741Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei486 – 4861Phosphoserine1 Publication
Glycosylationi742 – 7421N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ62668.
PRIDEiQ62668.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney. Isoform 3 and isoform 4 are expressed in both the inner and outer renal medulla.5 Publications

Gene expression databases

GenevestigatoriQ62668.

Interactioni

Subunit structurei

Isoform 1 interacts with SNAPIN which may be important for recruitment to the plasma membrane.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SNAPINO952956EBI-1635608,EBI-296723From a different organism.

Protein-protein interaction databases

BioGridi248522. 2 interactions.
IntActiQ62668. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ62668.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei133 – 15523HelicalSequence AnalysisAdd
BLAST
Transmembranei162 – 17918HelicalSequence AnalysisAdd
BLAST
Transmembranei184 – 20421HelicalSequence AnalysisAdd
BLAST
Transmembranei212 – 23221HelicalSequence AnalysisAdd
BLAST
Transmembranei241 – 26121HelicalSequence AnalysisAdd
BLAST
Transmembranei310 – 33021HelicalSequence AnalysisAdd
BLAST
Transmembranei349 – 37123HelicalSequence AnalysisAdd
BLAST
Transmembranei378 – 39922HelicalSequence AnalysisAdd
BLAST
Transmembranei400 – 42021HelicalSequence AnalysisAdd
BLAST
Transmembranei609 – 62921HelicalSequence AnalysisAdd
BLAST
Transmembranei647 – 66721HelicalSequence AnalysisAdd
BLAST
Transmembranei675 – 69521HelicalSequence AnalysisAdd
BLAST
Transmembranei704 – 72421HelicalSequence AnalysisAdd
BLAST
Transmembranei773 – 79321HelicalSequence AnalysisAdd
BLAST
Transmembranei812 – 83221HelicalSequence AnalysisAdd
BLAST
Transmembranei841 – 86121HelicalSequence AnalysisAdd
BLAST
Transmembranei863 – 88321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the urea transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4413.
HOGENOMiHOG000168437.
HOVERGENiHBG073255.
InParanoidiQ62668.
KOiK08716.
PhylomeDBiQ62668.

Family and domain databases

Gene3Di1.10.3430.10. 2 hits.
InterProiIPR029020. Ammonium/urea_transptr.
IPR004937. Urea_transporter.
[Graphical view]
PANTHERiPTHR10464. PTHR10464. 1 hit.
PfamiPF03253. UT. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q62668-1) [UniParc]FASTAAdd to Basket

Also known as: UT-A1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDHPLKEMS DNNRSPPLPE PLSSRYKLYE SELSSPTWPS SSQDTHPALP
60 70 80 90 100
LLEMPEEKDL RSSDEDSHIV KIEKPNERSK RRESELPRRA SAGRGAFSLF
110 120 130 140 150
QAVSYLTGDM KECKNWLKDK PLVLQFLDWV LRGAAQVMFV NNPLSGLIIF
160 170 180 190 200
IGLLIQNPWW TIAGALGTVV STLAALALSQ DRSAIASGLH GYNGMLVGLL
210 220 230 240 250
VAVFSEKLDY YWWLLFPVTF ASMACPVISS ALSTVFAKWD LPVFTLPFNI
260 270 280 290 300
ALTLYLAATG HYNLFFPTTL VKPASSAPNI TWSEIEMPLL LQTIPVGVGQ
310 320 330 340 350
VYGCDNPWTG GVILVALFIS SPLICLHAAI GSIVGLLAAL TVATPFETIY
360 370 380 390 400
TGLWSYNCVL SCVAIGGMFY VLTWQTHLLA LVCALFCAYT GAALSNMMAV
410 420 430 440 450
VGVPPGTWAF CLSTLTFLLL TSNNPGIHKL PLSKVTYPEA NRIYFLTAKR
460 470 480 490 500
SDEQKPPNGG GGEQSHGGGQ RKAEEGSETV FPRRKSVFHI EWSSIRRRSK
510 520 530 540 550
VFGKSEHQER QTKEPLPYLY RKPTVELLDL NTMEESSEIK VETNTTRTTW
560 570 580 590 600
IQSSMIAGGK RVSKALSYIT GEMKECGEGL KDKSPVFQFL DWVLRGTSQV
610 620 630 640 650
MFVNNPLSGI LIVLGLFVQN PWWAISGCLG TIMSTLTALI LSQDKSAIAA
660 670 680 690 700
GLHGYNGVLV GLLMAVFSDK GNYYWWLLLP VIVMSMTCPI LSSALSTVFS
710 720 730 740 750
KWDLPVFTLP FNIAVTLYLA ATGHYNLFFP TKLLQPAVTT PNITWSDVQV
760 770 780 790 800
PLLLRAIPVG IGQVYGCDNP WTGGIFLVAL FVSSPLICLH AAIGSTIGML
810 820 830 840 850
AALSIATPFD SIYFGLCGFN STLACIAIGG MFYVITWQTH LLAIACALFA
860 870 880 890 900
AYLGAALANM LSVFGLPPCT WPFCLSALTF LLLTTNNPGI YKLPLSKVTY
910 920
PEANRIYFLS QEKNRRASMI TKYQAYDVS
Length:929
Mass (Da):101,896
Last modified:February 5, 2008 - v2
Checksum:i1D12D9D27764AC26
GO
Isoform 2 (identifier: Q62668-2) [UniParc]FASTAAdd to Basket

Also known as: UT-A2

The sequence of this isoform differs from the canonical sequence as follows:
     1-532: Missing.

Show »
Length:397
Mass (Da):43,237
Checksum:iEA2068EA25661224
GO
Isoform 3 (identifier: Q62668-3) [UniParc]FASTAAdd to Basket

Also known as: UT-A3

The sequence of this isoform differs from the canonical sequence as follows:
     461-929: Missing.

Show »
Length:460
Mass (Da):50,328
Checksum:i38AAEB61F779BC31
GO
Isoform 4 (identifier: Q62668-4) [UniParc]FASTAAdd to Basket

Also known as: UT-A4

The sequence of this isoform differs from the canonical sequence as follows:
     252-714: Missing.

Show »
Length:466
Mass (Da):51,258
Checksum:iCD8EB1A9C1A46BD3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171P → L in AAD23098. (PubMed:10215321)Curated
Sequence conflicti17 – 171P → L in AAD23099. (PubMed:10215321)Curated
Sequence conflicti96 – 961A → G in AAD23098. (PubMed:10215321)Curated
Sequence conflicti96 – 961A → G in AAD23099. (PubMed:10215321)Curated
Sequence conflicti460 – 4601G → D in AAD23098. (PubMed:10215321)Curated
Sequence conflicti460 – 4601G → D in AAD01938. (PubMed:11181411)Curated
Sequence conflicti780 – 7801L → P in AAD23099. (PubMed:10215321)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 532532Missing in isoform 2. 1 PublicationVSP_031172Add
BLAST
Alternative sequencei252 – 714463Missing in isoform 4. 1 PublicationVSP_031173Add
BLAST
Alternative sequencei461 – 929469Missing in isoform 3. 2 PublicationsVSP_031174Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09957 mRNA. Translation: AAA84392.1.
U77971 mRNA. Translation: AAB50197.1.
AF041788 mRNA. Translation: AAD23098.1.
AF042167 mRNA. Translation: AAD23099.1.
AF031642 mRNA. Translation: AAD01938.1.
RefSeqiNP_001103740.1. NM_001110270.1. [Q62668-2]
NP_062220.2. NM_019347.2.
NP_808877.2. NM_177962.3.
UniGeneiRn.10157.

Genome annotation databases

GeneIDi54302.
KEGGirno:54302.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09957 mRNA. Translation: AAA84392.1 .
U77971 mRNA. Translation: AAB50197.1 .
AF041788 mRNA. Translation: AAD23098.1 .
AF042167 mRNA. Translation: AAD23099.1 .
AF031642 mRNA. Translation: AAD01938.1 .
RefSeqi NP_001103740.1. NM_001110270.1. [Q62668-2 ]
NP_062220.2. NM_019347.2.
NP_808877.2. NM_177962.3.
UniGenei Rn.10157.

3D structure databases

ProteinModelPortali Q62668.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248522. 2 interactions.
IntActi Q62668. 5 interactions.

Protein family/group databases

TCDBi 1.A.28.1.1. the urea transporter (ut) family.

Proteomic databases

PaxDbi Q62668.
PRIDEi Q62668.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 54302.
KEGGi rno:54302.

Organism-specific databases

CTDi 8170.
RGDi 3689. Slc14a2.

Phylogenomic databases

eggNOGi COG4413.
HOGENOMi HOG000168437.
HOVERGENi HBG073255.
InParanoidi Q62668.
KOi K08716.
PhylomeDBi Q62668.

Miscellaneous databases

NextBioi 610950.
PROi Q62668.

Gene expression databases

Genevestigatori Q62668.

Family and domain databases

Gene3Di 1.10.3430.10. 2 hits.
InterProi IPR029020. Ammonium/urea_transptr.
IPR004937. Urea_transporter.
[Graphical view ]
PANTHERi PTHR10464. PTHR10464. 1 hit.
Pfami PF03253. UT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and regulation of expression of the rat kidney urea transporter (rUT2)."
    Smith C.P., Lee W.-S., Martial S., Knepper M.A., You G., Sands J.M., Hediger M.A.
    J. Clin. Invest. 96:1556-1563(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, INDUCTION.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "Molecular cloning and characterization of the vasopressin-regulated urea transporter of rat kidney collecting ducts."
    Shayakul C., Steel A., Hediger M.A.
    J. Clin. Invest. 98:2580-2587(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Kidney inner medulla.
  3. "Cloning and characterization of two new isoforms of the rat kidney urea transporter: UT-A3 and UT-A4."
    Karakashian A., Timmer R.T., Klein J.D., Gunn R.B., Sands J.M., Bagnasco S.M.
    J. Am. Soc. Nephrol. 10:230-237(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), FUNCTION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Kidney inner medulla.
  4. "Molecular characterization of a novel urea transporter from kidney inner medullary collecting ducts."
    Shayakul C., Tsukaguchi H., Berger U.V., Hediger M.A.
    Am. J. Physiol. 280:F487-F494(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  5. "Vasopressin increases plasma membrane accumulation of urea transporter UT-A1 in rat inner medullary collecting ducts."
    Klein J.D., Froehlich O., Blount M.A., Martin C.F., Smith T.D., Sands J.M.
    J. Am. Soc. Nephrol. 17:2680-2686(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION.
  6. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-486, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "The UT-A1 urea transporter interacts with snapin, a SNARE-associated protein."
    Mistry A.C., Mallick R., Froehlich O., Klein J.D., Rehm A., Chen G., Sands J.M.
    J. Biol. Chem. 282:30097-30106(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNAPIN, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiUT2_RAT
AccessioniPrimary (citable) accession number: Q62668
Secondary accession number(s): Q9R1Y7, Q9WTT8, Q9Z2R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: October 29, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3