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Protein

Urea transporter 2

Gene

Slc14a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. Vasopressin regulates urea transport by increasing isoform 1 accumulation in the plasma membrane and/or phosphorylation of isoform 1.4 Publications

GO - Molecular functioni

  • urea channel activity Source: GO_Central
  • urea transmembrane transporter activity Source: RGD

GO - Biological processi

  • response to water deprivation Source: RGD
  • urea transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-RNO-425366. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Protein family/group databases

TCDBi1.A.28.1.1. the urea transporter (ut) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Urea transporter 2
Alternative name(s):
Solute carrier family 14 member 2
Urea transporter, kidney
Gene namesi
Name:Slc14a2
Synonyms:Ut2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi3689. Slc14a2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei133 – 155HelicalSequence analysisAdd BLAST23
Transmembranei162 – 179HelicalSequence analysisAdd BLAST18
Transmembranei184 – 204HelicalSequence analysisAdd BLAST21
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Transmembranei241 – 261HelicalSequence analysisAdd BLAST21
Transmembranei310 – 330HelicalSequence analysisAdd BLAST21
Transmembranei349 – 371HelicalSequence analysisAdd BLAST23
Transmembranei378 – 399HelicalSequence analysisAdd BLAST22
Transmembranei400 – 420HelicalSequence analysisAdd BLAST21
Transmembranei609 – 629HelicalSequence analysisAdd BLAST21
Transmembranei647 – 667HelicalSequence analysisAdd BLAST21
Transmembranei675 – 695HelicalSequence analysisAdd BLAST21
Transmembranei704 – 724HelicalSequence analysisAdd BLAST21
Transmembranei773 – 793HelicalSequence analysisAdd BLAST21
Transmembranei812 – 832HelicalSequence analysisAdd BLAST21
Transmembranei841 – 861HelicalSequence analysisAdd BLAST21
Transmembranei863 – 883HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657411 – 929Urea transporter 2Add BLAST929

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei486PhosphoserineCombined sources1
Glycosylationi742N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ62668.
PRIDEiQ62668.

PTM databases

iPTMnetiQ62668.

Expressioni

Tissue specificityi

Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney. Isoform 3 and isoform 4 are expressed in both the inner and outer renal medulla.5 Publications

Interactioni

Subunit structurei

Isoform 1 interacts with SNAPIN which may be important for recruitment to the plasma membrane.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SNAPINO952956EBI-1635608,EBI-296723From a different organism.

Protein-protein interaction databases

BioGridi248522. 2 interactors.
IntActiQ62668. 5 interactors.
STRINGi10116.ENSRNOP00000022417.

Chemistry databases

BindingDBiQ62668.

Structurei

3D structure databases

ProteinModelPortaliQ62668.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the urea transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEWG. Eukaryota.
COG4413. LUCA.
GeneTreeiENSGT00390000018729.
HOGENOMiHOG000168437.
HOVERGENiHBG073255.
InParanoidiQ62668.
KOiK08716.
OMAiWGYNCVL.
PhylomeDBiQ62668.

Family and domain databases

Gene3Di1.10.3430.10. 2 hits.
InterProiIPR029020. Ammonium/urea_transptr.
IPR004937. Urea_transporter.
[Graphical view]
PANTHERiPTHR10464. PTHR10464. 1 hit.
PfamiPF03253. UT. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62668-1) [UniParc]FASTAAdd to basket
Also known as: UT-A1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDHPLKEMS DNNRSPPLPE PLSSRYKLYE SELSSPTWPS SSQDTHPALP
60 70 80 90 100
LLEMPEEKDL RSSDEDSHIV KIEKPNERSK RRESELPRRA SAGRGAFSLF
110 120 130 140 150
QAVSYLTGDM KECKNWLKDK PLVLQFLDWV LRGAAQVMFV NNPLSGLIIF
160 170 180 190 200
IGLLIQNPWW TIAGALGTVV STLAALALSQ DRSAIASGLH GYNGMLVGLL
210 220 230 240 250
VAVFSEKLDY YWWLLFPVTF ASMACPVISS ALSTVFAKWD LPVFTLPFNI
260 270 280 290 300
ALTLYLAATG HYNLFFPTTL VKPASSAPNI TWSEIEMPLL LQTIPVGVGQ
310 320 330 340 350
VYGCDNPWTG GVILVALFIS SPLICLHAAI GSIVGLLAAL TVATPFETIY
360 370 380 390 400
TGLWSYNCVL SCVAIGGMFY VLTWQTHLLA LVCALFCAYT GAALSNMMAV
410 420 430 440 450
VGVPPGTWAF CLSTLTFLLL TSNNPGIHKL PLSKVTYPEA NRIYFLTAKR
460 470 480 490 500
SDEQKPPNGG GGEQSHGGGQ RKAEEGSETV FPRRKSVFHI EWSSIRRRSK
510 520 530 540 550
VFGKSEHQER QTKEPLPYLY RKPTVELLDL NTMEESSEIK VETNTTRTTW
560 570 580 590 600
IQSSMIAGGK RVSKALSYIT GEMKECGEGL KDKSPVFQFL DWVLRGTSQV
610 620 630 640 650
MFVNNPLSGI LIVLGLFVQN PWWAISGCLG TIMSTLTALI LSQDKSAIAA
660 670 680 690 700
GLHGYNGVLV GLLMAVFSDK GNYYWWLLLP VIVMSMTCPI LSSALSTVFS
710 720 730 740 750
KWDLPVFTLP FNIAVTLYLA ATGHYNLFFP TKLLQPAVTT PNITWSDVQV
760 770 780 790 800
PLLLRAIPVG IGQVYGCDNP WTGGIFLVAL FVSSPLICLH AAIGSTIGML
810 820 830 840 850
AALSIATPFD SIYFGLCGFN STLACIAIGG MFYVITWQTH LLAIACALFA
860 870 880 890 900
AYLGAALANM LSVFGLPPCT WPFCLSALTF LLLTTNNPGI YKLPLSKVTY
910 920
PEANRIYFLS QEKNRRASMI TKYQAYDVS
Length:929
Mass (Da):101,896
Last modified:February 5, 2008 - v2
Checksum:i1D12D9D27764AC26
GO
Isoform 2 (identifier: Q62668-2) [UniParc]FASTAAdd to basket
Also known as: UT-A2

The sequence of this isoform differs from the canonical sequence as follows:
     1-532: Missing.

Show »
Length:397
Mass (Da):43,237
Checksum:iEA2068EA25661224
GO
Isoform 3 (identifier: Q62668-3) [UniParc]FASTAAdd to basket
Also known as: UT-A3

The sequence of this isoform differs from the canonical sequence as follows:
     461-929: Missing.

Show »
Length:460
Mass (Da):50,328
Checksum:i38AAEB61F779BC31
GO
Isoform 4 (identifier: Q62668-4) [UniParc]FASTAAdd to basket
Also known as: UT-A4

The sequence of this isoform differs from the canonical sequence as follows:
     252-714: Missing.

Show »
Length:466
Mass (Da):51,258
Checksum:iCD8EB1A9C1A46BD3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17P → L in AAD23098 (PubMed:10215321).Curated1
Sequence conflicti17P → L in AAD23099 (PubMed:10215321).Curated1
Sequence conflicti96A → G in AAD23098 (PubMed:10215321).Curated1
Sequence conflicti96A → G in AAD23099 (PubMed:10215321).Curated1
Sequence conflicti460G → D in AAD23098 (PubMed:10215321).Curated1
Sequence conflicti460G → D in AAD01938 (PubMed:11181411).Curated1
Sequence conflicti780L → P in AAD23099 (PubMed:10215321).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0311721 – 532Missing in isoform 2. 1 PublicationAdd BLAST532
Alternative sequenceiVSP_031173252 – 714Missing in isoform 4. 1 PublicationAdd BLAST463
Alternative sequenceiVSP_031174461 – 929Missing in isoform 3. 2 PublicationsAdd BLAST469

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09957 mRNA. Translation: AAA84392.1.
U77971 mRNA. Translation: AAB50197.1.
AF041788 mRNA. Translation: AAD23098.1.
AF042167 mRNA. Translation: AAD23099.1.
AF031642 mRNA. Translation: AAD01938.1.
RefSeqiNP_001103740.1. NM_001110270.1. [Q62668-2]
NP_062220.2. NM_019347.2.
NP_808877.2. NM_177962.3.
UniGeneiRn.10157.

Genome annotation databases

EnsembliENSRNOT00000078519; ENSRNOP00000074116; ENSRNOG00000056021. [Q62668-2]
GeneIDi54302.
KEGGirno:54302.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09957 mRNA. Translation: AAA84392.1.
U77971 mRNA. Translation: AAB50197.1.
AF041788 mRNA. Translation: AAD23098.1.
AF042167 mRNA. Translation: AAD23099.1.
AF031642 mRNA. Translation: AAD01938.1.
RefSeqiNP_001103740.1. NM_001110270.1. [Q62668-2]
NP_062220.2. NM_019347.2.
NP_808877.2. NM_177962.3.
UniGeneiRn.10157.

3D structure databases

ProteinModelPortaliQ62668.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248522. 2 interactors.
IntActiQ62668. 5 interactors.
STRINGi10116.ENSRNOP00000022417.

Chemistry databases

BindingDBiQ62668.

Protein family/group databases

TCDBi1.A.28.1.1. the urea transporter (ut) family.

PTM databases

iPTMnetiQ62668.

Proteomic databases

PaxDbiQ62668.
PRIDEiQ62668.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000078519; ENSRNOP00000074116; ENSRNOG00000056021. [Q62668-2]
GeneIDi54302.
KEGGirno:54302.

Organism-specific databases

CTDi8170.
RGDi3689. Slc14a2.

Phylogenomic databases

eggNOGiENOG410IEWG. Eukaryota.
COG4413. LUCA.
GeneTreeiENSGT00390000018729.
HOGENOMiHOG000168437.
HOVERGENiHBG073255.
InParanoidiQ62668.
KOiK08716.
OMAiWGYNCVL.
PhylomeDBiQ62668.

Enzyme and pathway databases

ReactomeiR-RNO-425366. Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds.

Miscellaneous databases

PROiQ62668.

Family and domain databases

Gene3Di1.10.3430.10. 2 hits.
InterProiIPR029020. Ammonium/urea_transptr.
IPR004937. Urea_transporter.
[Graphical view]
PANTHERiPTHR10464. PTHR10464. 1 hit.
PfamiPF03253. UT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUT2_RAT
AccessioniPrimary (citable) accession number: Q62668
Secondary accession number(s): Q9R1Y7, Q9WTT8, Q9Z2R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.