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Q62668

- UT2_RAT

UniProt

Q62668 - UT2_RAT

Protein

Urea transporter 2

Gene

Slc14a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (05 Feb 2008)
      Previous versions | rss
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    Functioni

    Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. Vasopressin regulates urea transport by increasing isoform 1 accumulation in the plasma membrane and/or phosphorylation of isoform 1.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. urea transmembrane transporter activity Source: RGD

    GO - Biological processi

    1. response to water deprivation Source: RGD
    2. urea transport Source: RGD

    Keywords - Biological processi

    Transport

    Protein family/group databases

    TCDBi1.A.28.1.1. the urea transporter (ut) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Urea transporter 2
    Alternative name(s):
    Solute carrier family 14 member 2
    Urea transporter, kidney
    Gene namesi
    Name:Slc14a2
    Synonyms:Ut2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3689. Slc14a2.

    Subcellular locationi

    Apical cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 929929Urea transporter 2PRO_0000065741Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei486 – 4861Phosphoserine2 Publications
    Glycosylationi742 – 7421N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ62668.
    PRIDEiQ62668.

    Expressioni

    Tissue specificityi

    Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney. Isoform 3 and isoform 4 are expressed in both the inner and outer renal medulla.5 Publications

    Gene expression databases

    GenevestigatoriQ62668.

    Interactioni

    Subunit structurei

    Isoform 1 interacts with SNAPIN which may be important for recruitment to the plasma membrane.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SNAPINO952956EBI-1635608,EBI-296723From a different organism.

    Protein-protein interaction databases

    BioGridi248522. 2 interactions.
    IntActiQ62668. 5 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62668.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei133 – 15523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei162 – 17918HelicalSequence AnalysisAdd
    BLAST
    Transmembranei184 – 20421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei212 – 23221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei241 – 26121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei310 – 33021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei349 – 37123HelicalSequence AnalysisAdd
    BLAST
    Transmembranei378 – 39922HelicalSequence AnalysisAdd
    BLAST
    Transmembranei400 – 42021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei609 – 62921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei647 – 66721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei675 – 69521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei704 – 72421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei773 – 79321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei812 – 83221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei841 – 86121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei863 – 88321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the urea transporter family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4413.
    HOGENOMiHOG000168437.
    HOVERGENiHBG073255.
    InParanoidiQ9R1Y7.
    KOiK08716.
    PhylomeDBiQ62668.

    Family and domain databases

    Gene3Di1.10.3430.10. 2 hits.
    InterProiIPR029020. Ammonium/urea_transptr.
    IPR004937. Urea_transporter.
    [Graphical view]
    PANTHERiPTHR10464. PTHR10464. 1 hit.
    PfamiPF03253. UT. 2 hits.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q62668-1) [UniParc]FASTAAdd to Basket

    Also known as: UT-A1

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSDHPLKEMS DNNRSPPLPE PLSSRYKLYE SELSSPTWPS SSQDTHPALP    50
    LLEMPEEKDL RSSDEDSHIV KIEKPNERSK RRESELPRRA SAGRGAFSLF 100
    QAVSYLTGDM KECKNWLKDK PLVLQFLDWV LRGAAQVMFV NNPLSGLIIF 150
    IGLLIQNPWW TIAGALGTVV STLAALALSQ DRSAIASGLH GYNGMLVGLL 200
    VAVFSEKLDY YWWLLFPVTF ASMACPVISS ALSTVFAKWD LPVFTLPFNI 250
    ALTLYLAATG HYNLFFPTTL VKPASSAPNI TWSEIEMPLL LQTIPVGVGQ 300
    VYGCDNPWTG GVILVALFIS SPLICLHAAI GSIVGLLAAL TVATPFETIY 350
    TGLWSYNCVL SCVAIGGMFY VLTWQTHLLA LVCALFCAYT GAALSNMMAV 400
    VGVPPGTWAF CLSTLTFLLL TSNNPGIHKL PLSKVTYPEA NRIYFLTAKR 450
    SDEQKPPNGG GGEQSHGGGQ RKAEEGSETV FPRRKSVFHI EWSSIRRRSK 500
    VFGKSEHQER QTKEPLPYLY RKPTVELLDL NTMEESSEIK VETNTTRTTW 550
    IQSSMIAGGK RVSKALSYIT GEMKECGEGL KDKSPVFQFL DWVLRGTSQV 600
    MFVNNPLSGI LIVLGLFVQN PWWAISGCLG TIMSTLTALI LSQDKSAIAA 650
    GLHGYNGVLV GLLMAVFSDK GNYYWWLLLP VIVMSMTCPI LSSALSTVFS 700
    KWDLPVFTLP FNIAVTLYLA ATGHYNLFFP TKLLQPAVTT PNITWSDVQV 750
    PLLLRAIPVG IGQVYGCDNP WTGGIFLVAL FVSSPLICLH AAIGSTIGML 800
    AALSIATPFD SIYFGLCGFN STLACIAIGG MFYVITWQTH LLAIACALFA 850
    AYLGAALANM LSVFGLPPCT WPFCLSALTF LLLTTNNPGI YKLPLSKVTY 900
    PEANRIYFLS QEKNRRASMI TKYQAYDVS 929
    Length:929
    Mass (Da):101,896
    Last modified:February 5, 2008 - v2
    Checksum:i1D12D9D27764AC26
    GO
    Isoform 2 (identifier: Q62668-2) [UniParc]FASTAAdd to Basket

    Also known as: UT-A2

    The sequence of this isoform differs from the canonical sequence as follows:
         1-532: Missing.

    Show »
    Length:397
    Mass (Da):43,237
    Checksum:iEA2068EA25661224
    GO
    Isoform 3 (identifier: Q62668-3) [UniParc]FASTAAdd to Basket

    Also known as: UT-A3

    The sequence of this isoform differs from the canonical sequence as follows:
         461-929: Missing.

    Show »
    Length:460
    Mass (Da):50,328
    Checksum:i38AAEB61F779BC31
    GO
    Isoform 4 (identifier: Q62668-4) [UniParc]FASTAAdd to Basket

    Also known as: UT-A4

    The sequence of this isoform differs from the canonical sequence as follows:
         252-714: Missing.

    Show »
    Length:466
    Mass (Da):51,258
    Checksum:iCD8EB1A9C1A46BD3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171P → L in AAD23098. (PubMed:10215321)Curated
    Sequence conflicti17 – 171P → L in AAD23099. (PubMed:10215321)Curated
    Sequence conflicti96 – 961A → G in AAD23098. (PubMed:10215321)Curated
    Sequence conflicti96 – 961A → G in AAD23099. (PubMed:10215321)Curated
    Sequence conflicti460 – 4601G → D in AAD23098. (PubMed:10215321)Curated
    Sequence conflicti460 – 4601G → D in AAD01938. (PubMed:11181411)Curated
    Sequence conflicti780 – 7801L → P in AAD23099. (PubMed:10215321)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 532532Missing in isoform 2. 1 PublicationVSP_031172Add
    BLAST
    Alternative sequencei252 – 714463Missing in isoform 4. 1 PublicationVSP_031173Add
    BLAST
    Alternative sequencei461 – 929469Missing in isoform 3. 2 PublicationsVSP_031174Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09957 mRNA. Translation: AAA84392.1.
    U77971 mRNA. Translation: AAB50197.1.
    AF041788 mRNA. Translation: AAD23098.1.
    AF042167 mRNA. Translation: AAD23099.1.
    AF031642 mRNA. Translation: AAD01938.1.
    RefSeqiNP_001103740.1. NM_001110270.1. [Q62668-2]
    NP_062220.2. NM_019347.2.
    NP_808877.2. NM_177962.3.
    UniGeneiRn.10157.

    Genome annotation databases

    GeneIDi54302.
    KEGGirno:54302.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09957 mRNA. Translation: AAA84392.1 .
    U77971 mRNA. Translation: AAB50197.1 .
    AF041788 mRNA. Translation: AAD23098.1 .
    AF042167 mRNA. Translation: AAD23099.1 .
    AF031642 mRNA. Translation: AAD01938.1 .
    RefSeqi NP_001103740.1. NM_001110270.1. [Q62668-2 ]
    NP_062220.2. NM_019347.2.
    NP_808877.2. NM_177962.3.
    UniGenei Rn.10157.

    3D structure databases

    ProteinModelPortali Q62668.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248522. 2 interactions.
    IntActi Q62668. 5 interactions.

    Protein family/group databases

    TCDBi 1.A.28.1.1. the urea transporter (ut) family.

    Proteomic databases

    PaxDbi Q62668.
    PRIDEi Q62668.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 54302.
    KEGGi rno:54302.

    Organism-specific databases

    CTDi 8170.
    RGDi 3689. Slc14a2.

    Phylogenomic databases

    eggNOGi COG4413.
    HOGENOMi HOG000168437.
    HOVERGENi HBG073255.
    InParanoidi Q9R1Y7.
    KOi K08716.
    PhylomeDBi Q62668.

    Miscellaneous databases

    NextBioi 610950.
    PROi Q62668.

    Gene expression databases

    Genevestigatori Q62668.

    Family and domain databases

    Gene3Di 1.10.3430.10. 2 hits.
    InterProi IPR029020. Ammonium/urea_transptr.
    IPR004937. Urea_transporter.
    [Graphical view ]
    PANTHERi PTHR10464. PTHR10464. 1 hit.
    Pfami PF03253. UT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and regulation of expression of the rat kidney urea transporter (rUT2)."
      Smith C.P., Lee W.-S., Martial S., Knepper M.A., You G., Sands J.M., Hediger M.A.
      J. Clin. Invest. 96:1556-1563(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, INDUCTION.
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    2. "Molecular cloning and characterization of the vasopressin-regulated urea transporter of rat kidney collecting ducts."
      Shayakul C., Steel A., Hediger M.A.
      J. Clin. Invest. 98:2580-2587(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Kidney inner medulla.
    3. "Cloning and characterization of two new isoforms of the rat kidney urea transporter: UT-A3 and UT-A4."
      Karakashian A., Timmer R.T., Klein J.D., Gunn R.B., Sands J.M., Bagnasco S.M.
      J. Am. Soc. Nephrol. 10:230-237(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), FUNCTION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Kidney inner medulla.
    4. "Molecular characterization of a novel urea transporter from kidney inner medullary collecting ducts."
      Shayakul C., Tsukaguchi H., Berger U.V., Hediger M.A.
      Am. J. Physiol. 280:F487-F494(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    5. "Vasopressin increases plasma membrane accumulation of urea transporter UT-A1 in rat inner medullary collecting ducts."
      Klein J.D., Froehlich O., Blount M.A., Martin C.F., Smith T.D., Sands J.M.
      J. Am. Soc. Nephrol. 17:2680-2686(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION.
    6. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
      Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
      Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-486, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "The UT-A1 urea transporter interacts with snapin, a SNARE-associated protein."
      Mistry A.C., Mallick R., Froehlich O., Klein J.D., Rehm A., Chen G., Sands J.M.
      J. Biol. Chem. 282:30097-30106(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNAPIN, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiUT2_RAT
    AccessioniPrimary (citable) accession number: Q62668
    Secondary accession number(s): Q9R1Y7, Q9WTT8, Q9Z2R3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 5, 2008
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-9 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3