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Q62668 (UT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Urea transporter 2
Alternative name(s):
Solute carrier family 14 member 2
Urea transporter, kidney
Gene names
Name:Slc14a2
Synonyms:Ut2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length929 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. Vasopressin regulates urea transport by increasing isoform 1 accumulation in the plasma membrane and/or phosphorylation of isoform 1. Ref.2 Ref.3 Ref.4 Ref.5

Subunit structure

Isoform 1 interacts with SNAPIN which may be important for recruitment to the plasma membrane. Ref.7

Subcellular location

Apical cell membrane; Multi-pass membrane protein Ref.7.

Tissue specificity

Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney. Isoform 3 and isoform 4 are expressed in both the inner and outer renal medulla. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5

Sequence similarities

Belongs to the urea transporter family.

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SNAPINO952956EBI-1635608,EBI-296723From a different organism.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q62668-1)

Also known as: UT-A1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q62668-2)

Also known as: UT-A2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-532: Missing.
Isoform 3 (identifier: Q62668-3)

Also known as: UT-A3;

The sequence of this isoform differs from the canonical sequence as follows:
     461-929: Missing.
Isoform 4 (identifier: Q62668-4)

Also known as: UT-A4;

The sequence of this isoform differs from the canonical sequence as follows:
     252-714: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 929929Urea transporter 2
PRO_0000065741

Regions

Transmembrane133 – 15523Helical; Potential
Transmembrane162 – 17918Helical; Potential
Transmembrane184 – 20421Helical; Potential
Transmembrane212 – 23221Helical; Potential
Transmembrane241 – 26121Helical; Potential
Transmembrane310 – 33021Helical; Potential
Transmembrane349 – 37123Helical; Potential
Transmembrane378 – 39922Helical; Potential
Transmembrane400 – 42021Helical; Potential
Transmembrane609 – 62921Helical; Potential
Transmembrane647 – 66721Helical; Potential
Transmembrane675 – 69521Helical; Potential
Transmembrane704 – 72421Helical; Potential
Transmembrane773 – 79321Helical; Potential
Transmembrane812 – 83221Helical; Potential
Transmembrane841 – 86121Helical; Potential
Transmembrane863 – 88321Helical; Potential

Amino acid modifications

Modified residue4861Phosphoserine Ref.6
Glycosylation7421N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 532532Missing in isoform 2.
VSP_031172
Alternative sequence252 – 714463Missing in isoform 4.
VSP_031173
Alternative sequence461 – 929469Missing in isoform 3.
VSP_031174

Experimental info

Sequence conflict171P → L in AAD23098. Ref.3
Sequence conflict171P → L in AAD23099. Ref.3
Sequence conflict961A → G in AAD23098. Ref.3
Sequence conflict961A → G in AAD23099. Ref.3
Sequence conflict4601G → D in AAD23098. Ref.3
Sequence conflict4601G → D in AAD01938. Ref.4
Sequence conflict7801L → P in AAD23099. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (UT-A1) [UniParc].

Last modified February 5, 2008. Version 2.
Checksum: 1D12D9D27764AC26

FASTA929101,896
        10         20         30         40         50         60 
MSDHPLKEMS DNNRSPPLPE PLSSRYKLYE SELSSPTWPS SSQDTHPALP LLEMPEEKDL 

        70         80         90        100        110        120 
RSSDEDSHIV KIEKPNERSK RRESELPRRA SAGRGAFSLF QAVSYLTGDM KECKNWLKDK 

       130        140        150        160        170        180 
PLVLQFLDWV LRGAAQVMFV NNPLSGLIIF IGLLIQNPWW TIAGALGTVV STLAALALSQ 

       190        200        210        220        230        240 
DRSAIASGLH GYNGMLVGLL VAVFSEKLDY YWWLLFPVTF ASMACPVISS ALSTVFAKWD 

       250        260        270        280        290        300 
LPVFTLPFNI ALTLYLAATG HYNLFFPTTL VKPASSAPNI TWSEIEMPLL LQTIPVGVGQ 

       310        320        330        340        350        360 
VYGCDNPWTG GVILVALFIS SPLICLHAAI GSIVGLLAAL TVATPFETIY TGLWSYNCVL 

       370        380        390        400        410        420 
SCVAIGGMFY VLTWQTHLLA LVCALFCAYT GAALSNMMAV VGVPPGTWAF CLSTLTFLLL 

       430        440        450        460        470        480 
TSNNPGIHKL PLSKVTYPEA NRIYFLTAKR SDEQKPPNGG GGEQSHGGGQ RKAEEGSETV 

       490        500        510        520        530        540 
FPRRKSVFHI EWSSIRRRSK VFGKSEHQER QTKEPLPYLY RKPTVELLDL NTMEESSEIK 

       550        560        570        580        590        600 
VETNTTRTTW IQSSMIAGGK RVSKALSYIT GEMKECGEGL KDKSPVFQFL DWVLRGTSQV 

       610        620        630        640        650        660 
MFVNNPLSGI LIVLGLFVQN PWWAISGCLG TIMSTLTALI LSQDKSAIAA GLHGYNGVLV 

       670        680        690        700        710        720 
GLLMAVFSDK GNYYWWLLLP VIVMSMTCPI LSSALSTVFS KWDLPVFTLP FNIAVTLYLA 

       730        740        750        760        770        780 
ATGHYNLFFP TKLLQPAVTT PNITWSDVQV PLLLRAIPVG IGQVYGCDNP WTGGIFLVAL 

       790        800        810        820        830        840 
FVSSPLICLH AAIGSTIGML AALSIATPFD SIYFGLCGFN STLACIAIGG MFYVITWQTH 

       850        860        870        880        890        900 
LLAIACALFA AYLGAALANM LSVFGLPPCT WPFCLSALTF LLLTTNNPGI YKLPLSKVTY 

       910        920 
PEANRIYFLS QEKNRRASMI TKYQAYDVS 

« Hide

Isoform 2 (UT-A2) [UniParc].

Checksum: EA2068EA25661224
Show »

FASTA39743,237
Isoform 3 (UT-A3) [UniParc].

Checksum: 38AAEB61F779BC31
Show »

FASTA46050,328
Isoform 4 (UT-A4) [UniParc].

Checksum: CD8EB1A9C1A46BD3
Show »

FASTA46651,258

References

« Hide 'large scale' references
[1]"Cloning and regulation of expression of the rat kidney urea transporter (rUT2)."
Smith C.P., Lee W.-S., Martial S., Knepper M.A., You G., Sands J.M., Hediger M.A.
J. Clin. Invest. 96:1556-1563(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, INDUCTION.
Strain: Sprague-Dawley.
Tissue: Kidney.
[2]"Molecular cloning and characterization of the vasopressin-regulated urea transporter of rat kidney collecting ducts."
Shayakul C., Steel A., Hediger M.A.
J. Clin. Invest. 98:2580-2587(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Kidney inner medulla.
[3]"Cloning and characterization of two new isoforms of the rat kidney urea transporter: UT-A3 and UT-A4."
Karakashian A., Timmer R.T., Klein J.D., Gunn R.B., Sands J.M., Bagnasco S.M.
J. Am. Soc. Nephrol. 10:230-237(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), FUNCTION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Kidney inner medulla.
[4]"Molecular characterization of a novel urea transporter from kidney inner medullary collecting ducts."
Shayakul C., Tsukaguchi H., Berger U.V., Hediger M.A.
Am. J. Physiol. 280:F487-F494(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Kidney.
[5]"Vasopressin increases plasma membrane accumulation of urea transporter UT-A1 in rat inner medullary collecting ducts."
Klein J.D., Froehlich O., Blount M.A., Martin C.F., Smith T.D., Sands J.M.
J. Am. Soc. Nephrol. 17:2680-2686(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION.
[6]"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-486, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"The UT-A1 urea transporter interacts with snapin, a SNARE-associated protein."
Mistry A.C., Mallick R., Froehlich O., Klein J.D., Rehm A., Chen G., Sands J.M.
J. Biol. Chem. 282:30097-30106(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SNAPIN, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09957 mRNA. Translation: AAA84392.1.
U77971 mRNA. Translation: AAB50197.1.
AF041788 mRNA. Translation: AAD23098.1.
AF042167 mRNA. Translation: AAD23099.1.
AF031642 mRNA. Translation: AAD01938.1.
RefSeqNP_001103740.1. NM_001110270.1.
NP_062220.2. NM_019347.2.
NP_808877.2. NM_177962.3.
UniGeneRn.10157.

3D structure databases

ProteinModelPortalQ62668.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248522. 2 interactions.
IntActQ62668. 5 interactions.

Protein family/group databases

TCDB1.A.28.1.1. the urea transporter (ut) family.

Proteomic databases

PaxDbQ62668.
PRIDEQ62668.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID54302.
KEGGrno:54302.

Organism-specific databases

CTD8170.
RGD3689. Slc14a2.

Phylogenomic databases

eggNOGCOG4413.
HOGENOMHOG000168437.
HOVERGENHBG073255.
InParanoidQ9R1Y7.
KOK08716.
PhylomeDBQ62668.

Gene expression databases

GenevestigatorQ62668.

Family and domain databases

InterProIPR004937. Urea_transporter.
[Graphical view]
PANTHERPTHR10464. PTHR10464. 1 hit.
PfamPF03253. UT. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio610950.
PROQ62668.

Entry information

Entry nameUT2_RAT
AccessionPrimary (citable) accession number: Q62668
Secondary accession number(s): Q9R1Y7, Q9WTT8, Q9Z2R3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families