Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q62667

- MVP_RAT

UniProt

Q62667 - MVP_RAT

Protein

Major vault protein

Gene

Mvp

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. cell proliferation Source: Ensembl
    2. ERBB signaling pathway Source: Ensembl
    3. negative regulation of protein autophosphorylation Source: Ensembl
    4. negative regulation of protein tyrosine kinase activity Source: Ensembl
    5. negative regulation of signaling Source: Ensembl
    6. protein activation cascade Source: Ensembl

    Keywords - Molecular functioni

    Ribonucleoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Major vault protein
    Short name:
    MVP
    Gene namesi
    Name:Mvp
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi70932. Mvp.

    Subcellular locationi

    Cytoplasm. Nucleus By similarity

    GO - Cellular componenti

    1. cytoskeleton Source: Ensembl
    2. extracellular vesicular exosome Source: Ensembl
    3. nucleus Source: UniProtKB-SubCell
    4. perinuclear region of cytoplasm Source: Ensembl
    5. ribonucleoprotein complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 861860Major vault proteinPRO_0000158982Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity

    Post-translational modificationi

    Phosphorylated on Tyr residues after EGF stimulation.By similarity
    Dephosphorylated by PTPN11.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ62667.
    PRIDEiQ62667.

    PTM databases

    PhosphoSiteiQ62667.

    Expressioni

    Gene expression databases

    ArrayExpressiQ62667.
    GenevestigatoriQ62667.

    Interactioni

    Subunit structurei

    The vault ribonucleoprotein particle is a huge (400 A x 670 A) cage structure of 12.9 MDa. It consists of a dimer of half-vaults, with each half-vault comprising 39 identical major vault protein (MVP) chains, PARP4 and one or more vault RNAs (vRNAs). Interacts with TEP1. Interacts with PTEN and activated MAPK1. The phosphorylated protein interacts with the SH2 domains of PTPN11 and SRC. Interacts with APEX1 By similarity. May interact with ZNF540 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NR3C1P041502EBI-918333,EBI-493507From a different organism.
    Nr3c1P065362EBI-918333,EBI-1187143

    Protein-protein interaction databases

    DIPiDIP-29532N.
    IntActiQ62667. 3 interactions.
    MINTiMINT-1775954.
    STRINGi10116.ENSRNOP00000027360.

    Structurei

    Secondary structure

    1
    861
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi19 – 213
    Beta strandi25 – 339
    Beta strandi38 – 414
    Beta strandi46 – 483
    Beta strandi51 – 533
    Beta strandi57 – 593
    Beta strandi61 – 633
    Beta strandi67 – 704
    Beta strandi79 – 813
    Beta strandi84 – 918
    Beta strandi93 – 975
    Beta strandi109 – 1113
    Helixi116 – 1183
    Beta strandi121 – 1244
    Beta strandi132 – 1354
    Beta strandi142 – 1454
    Beta strandi153 – 1553
    Beta strandi158 – 1614
    Helixi169 – 1713
    Beta strandi174 – 1763
    Beta strandi200 – 2023
    Beta strandi211 – 2144
    Beta strandi226 – 2305
    Beta strandi232 – 2365
    Beta strandi248 – 2514
    Turni253 – 2553
    Beta strandi257 – 2593
    Beta strandi265 – 2706
    Beta strandi273 – 2753
    Turni277 – 2793
    Beta strandi282 – 2865
    Beta strandi292 – 2943
    Beta strandi299 – 3024
    Beta strandi304 – 3085
    Beta strandi315 – 3206
    Beta strandi324 – 3263
    Beta strandi328 – 33912
    Beta strandi341 – 3433
    Beta strandi357 – 3626
    Beta strandi371 – 3788
    Beta strandi387 – 3926
    Turni393 – 3953
    Beta strandi398 – 4047
    Beta strandi412 – 4143
    Helixi422 – 4265
    Beta strandi459 – 4613
    Beta strandi466 – 4716
    Beta strandi473 – 4753
    Beta strandi478 – 4814
    Beta strandi486 – 4883
    Beta strandi493 – 4953
    Beta strandi498 – 5003
    Beta strandi503 – 5053
    Beta strandi510 – 5178
    Beta strandi519 – 5213
    Beta strandi522 – 5309
    Beta strandi536 – 54712
    Turni550 – 5534
    Helixi555 – 5595
    Helixi560 – 5623
    Beta strandi563 – 5664
    Helixi567 – 58317
    Helixi588 – 60215
    Turni603 – 6053
    Turni629 – 6313
    Beta strandi633 – 64715
    Helixi648 – 801154
    Helixi806 – 8094
    Helixi811 – 8133

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QZVX-ray9.00A/B1-861[»]
    2ZUOX-ray3.50A/B/C/D/E/F/G/H/I/J/K/L/M1-861[»]
    2ZV4X-ray3.50N/O/P/Q/R/S/T/U/V/W/X/Y/Z1-861[»]
    2ZV5X-ray3.50a/b/c/d/e/f/g/h/i/j/k/l/m1-861[»]
    4HL8X-ray3.50A1-861[»]
    ProteinModelPortaliQ62667.
    SMRiQ62667. Positions 1-779.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ62667.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati2 – 5655MVP 11 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Repeati57 – 11155MVP 21 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Repeati112 – 16453MVP 31 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Repeati165 – 21753MVP 41 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Repeati218 – 27255MVP 51 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Repeati273 – 32351MVP 61 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Repeati324 – 37956MVP 71 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Repeati380 – 45778MVP 81 PublicationPROSITE-ProRule annotationAdd
    BLAST
    Repeati458 – 52063MVP 91 PublicationPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    MVP 3 mediates interaction with PTEN.By similarity
    MVP 4 mediates interaction with PARP4.By similarity

    Sequence similaritiesi

    Contains 9 MVP (vault) repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG70525.
    GeneTreeiENSGT00390000008969.
    HOGENOMiHOG000255109.
    HOVERGENiHBG003499.
    InParanoidiQ62667.
    KOiK17266.
    OMAiQDPLADR.
    OrthoDBiEOG773XFB.
    PhylomeDBiQ62667.

    Family and domain databases

    InterProiIPR021870. MVP_shoulder.
    IPR002499. Vault_N.
    [Graphical view]
    PfamiPF11978. MVP_shoulder. 1 hit.
    PF01505. Vault. 5 hits.
    [Graphical view]
    PROSITEiPS51224. MVP. 8 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q62667-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATEEAIIRI PPYHYIHVLD QNSNVSRVEV GPKTYIRQDN ERVLFAPVRM    50
    VTVPPRHYCI VANPVSRDTQ SSVLFDITGQ VRLRHADQEI RLAQDPFPLY 100
    PGEVLEKDIT PLQVVLPNTA LHLKALLDFE DKNGDKVMAG DEWLFEGPGT 150
    YIPQKEVEVV EIIQATVIKQ NQALRLRARK ECFDREGKGR VTGEEWLVRS 200
    VGAYLPAVFE EVLDLVDAVI LTEKTALHLR ALQNFRDLRG VLHRTGEEWL 250
    VTVQDTEAHV PDVYEEVLGV VPITTLGPRH YCVILDPMGP DGKNQLGQKR 300
    VVKGEKSFFL QPGERLERGI QDVYVLSEQQ GLLLKALQPL EEGESEEKVS 350
    HQAGDCWLIR GPLEYVPSAK VEVVEERQAI PLDQNEGIYV QDVKTGKVRA 400
    VIGSTYMLTQ DEVLWEKELP SGVEELLNLG HDPLADRGQK GTAKPLQPSA 450
    PRNKTRVVSY RVPHNAAVQV YDYRAKRARV VFGPELVTLD PEEQFTVLSL 500
    SAGRPKRPHA RRALCLLLGP DFFTDVITIE TADHARLQLQ LAYNWHFELK 550
    NRNDPAEAAK LFSVPDFVGD ACKAIASRVR GAVASVTFDD FHKNSARIIR 600
    MAVFGFEMSE DTGPDGTLLP KARDQAVFPQ NGLVVSSVDV QSVEPVDQRT 650
    RDALQRSVQL AIEITTNSQE AAAKHEAQRL EQEARGRLER QKILDQSEAE 700
    KARKELLELE AMSMAVESTG NAKAEAESRA EAARIEGEGS VLQAKLKAQA 750
    LAIETEAELE RVKKVREMEL IYARAQLELE VSKAQQLANV EAKKFKEMTE 800
    ALGPGTIRDL AVAGPEMQVK LLQSLGLKST LITDGSSPIN LFSTAFGLLG 850
    LGSDGQPPAQ K 861
    Length:861
    Mass (Da):95,798
    Last modified:January 23, 2007 - v4
    Checksum:iE51604F295D49A93
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09870 mRNA. Translation: AAC52161.2.
    BC071174 mRNA. Translation: AAH71174.1.
    PIRiI53908.
    RefSeqiNP_073206.2. NM_022715.2.
    UniGeneiRn.10028.

    Genome annotation databases

    EnsembliENSRNOT00000027360; ENSRNOP00000027360; ENSRNOG00000020182.
    GeneIDi64681.
    KEGGirno:64681.
    UCSCiRGD:70932. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09870 mRNA. Translation: AAC52161.2 .
    BC071174 mRNA. Translation: AAH71174.1 .
    PIRi I53908.
    RefSeqi NP_073206.2. NM_022715.2.
    UniGenei Rn.10028.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2QZV X-ray 9.00 A/B 1-861 [» ]
    2ZUO X-ray 3.50 A/B/C/D/E/F/G/H/I/J/K/L/M 1-861 [» ]
    2ZV4 X-ray 3.50 N/O/P/Q/R/S/T/U/V/W/X/Y/Z 1-861 [» ]
    2ZV5 X-ray 3.50 a/b/c/d/e/f/g/h/i/j/k/l/m 1-861 [» ]
    4HL8 X-ray 3.50 A 1-861 [» ]
    ProteinModelPortali Q62667.
    SMRi Q62667. Positions 1-779.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29532N.
    IntActi Q62667. 3 interactions.
    MINTi MINT-1775954.
    STRINGi 10116.ENSRNOP00000027360.

    PTM databases

    PhosphoSitei Q62667.

    Proteomic databases

    PaxDbi Q62667.
    PRIDEi Q62667.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027360 ; ENSRNOP00000027360 ; ENSRNOG00000020182 .
    GeneIDi 64681.
    KEGGi rno:64681.
    UCSCi RGD:70932. rat.

    Organism-specific databases

    CTDi 9961.
    RGDi 70932. Mvp.

    Phylogenomic databases

    eggNOGi NOG70525.
    GeneTreei ENSGT00390000008969.
    HOGENOMi HOG000255109.
    HOVERGENi HBG003499.
    InParanoidi Q62667.
    KOi K17266.
    OMAi QDPLADR.
    OrthoDBi EOG773XFB.
    PhylomeDBi Q62667.

    Miscellaneous databases

    EvolutionaryTracei Q62667.
    NextBioi 613698.
    PROi Q62667.

    Gene expression databases

    ArrayExpressi Q62667.
    Genevestigatori Q62667.

    Family and domain databases

    InterProi IPR021870. MVP_shoulder.
    IPR002499. Vault_N.
    [Graphical view ]
    Pfami PF11978. MVP_shoulder. 1 hit.
    PF01505. Vault. 5 hits.
    [Graphical view ]
    PROSITEi PS51224. MVP. 8 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of a cDNA encoding the major vault protein from Rattus norvegicus."
      Kickhoefer V.A., Rome L.H.
      Gene 151:257-260(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Kickhoefer V.A.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    4. Cited for: ASSOCIATION WITH TEP1.
    5. "Draft crystal structure of the vault shell at 9-A resolution."
      Anderson D.H., Kickhoefer V.A., Sievers S.A., Rome L.H., Eisenberg D.
      PLoS Biol. 5:E318-E318(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (9.0 ANGSTROMS), SUBUNIT.
    6. Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS), SUBUNIT, MVP REPEATS.

    Entry informationi

    Entry nameiMVP_RAT
    AccessioniPrimary (citable) accession number: Q62667
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 97 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3