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Reviewed, UniProtKB/Swiss-Prot Q62666 (VACHT_RAT)

Last modified October 13, 2009. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Vesicular acetylcholine transporter
      Short name=VAChT
      Short name=rVAT
Alternative name(s):
    Solute carrier family 18 member 3
Gene names
Name: Slc18a3
Synonyms: Vacht, Vat
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Involved in acetylcholine transport into synaptic vesicles. Ref.3

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus. Ref.3 Ref.1 Ref.2

Sequence similarities

Belongs to the major facilitator superfamily. Vesicular transporter family.

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Ontologies

Keywords
   Biological processNeurotransmitter transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processneurotransmitter transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 530530Vesicular acetylcholine transporter
PRO_0000127520

Regions

Topological domain1 – 3333Cytoplasmic Potential
Transmembrane34 – 5421 Potential
Topological domain55 – 12571Lumenal, vesicle Potential
Transmembrane126 – 14621 Potential
Topological domain147 – 1526Cytoplasmic Potential
Transmembrane153 – 17321 Potential
Topological domain174 – 1829Lumenal, vesicle Potential
Transmembrane183 – 20321 Potential
Topological domain204 – 21310Cytoplasmic Potential
Transmembrane214 – 23421 Potential
Topological domain235 – 2428Lumenal, vesicle Potential
Transmembrane243 – 26321 Potential
Topological domain264 – 28926Cytoplasmic Potential
Transmembrane290 – 31021 Potential
Topological domain311 – 32515Lumenal, vesicle Potential
Transmembrane326 – 34621 Potential
Topological domain347 – 35610Cytoplasmic Potential
Transmembrane357 – 37721 Potential
Topological domain378 – 38811Lumenal, vesicle Potential
Transmembrane389 – 40921 Potential
Topological domain410 – 42213Cytoplasmic Potential
Transmembrane423 – 44321 Potential
Topological domain444 – 4474Lumenal, vesicle Potential
Transmembrane448 – 46821 Potential
Topological domain469 – 53062Cytoplasmic Potential

Amino acid modifications

Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation961N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict921A → T in AAA50831. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q62666-1 [UniParc].

Last modified September 19, 2003. Version 2.
Checksum: 07720DA749527438

FASTA53056,537
        10         20         30         40         50         60 
MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY MVIVPIVPDY 

        70         80         90        100        110        120 
IAHMRGGSEG PTLVSEVWEP TLPPPTLANA SAYLANTSAS PTAAGSARSI LRPRYPTESE 

       130        140        150        160        170        180 
DVKIGVLFAS KAILQLLVNP LSGPFIDRMS YDVPLLIGLG VMFASTVMFA FAEDYATLFA 

       190        200        210        220        230        240 
ARSLQGLGSA FADTSGIAMI ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG 

       250        260        270        280        290        300 
KRVPFLVLAA VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT 

       310        320        330        340        350        360 
CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA RYPHLQWLYG 

       370        380        390        400        410        420 
ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA LLPTLAFLVD VRHVSVYGSV 

       430        440        450        460        470        480 
YAIADISYSV AYALGPIVAG HIVHSLGFEQ LSLGMGLANL LYAPVLLLLR NVGLLTRSRS 

       490        500        510        520        530 
ERDVLLDEPP QGLYDAVRLR EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS

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References

[1]"Molecular cloning of a putative vesicular transporter for acetylcholine."
Roghani A., Feldman J., Kohan S.A., Shirzadi A., Gundersen C.B., Brecha N., Edwards R.H.
Proc. Natl. Acad. Sci. U.S.A. 91:10620-10624(1994) [PubMed: 7938002] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Brain stem.
[2]"Functional identification of a vesicular acetylcholine transporter and its expression from a 'cholinergic' gene locus."
Erickson J.D., Varoqui H., Schafer M.K.-H., Modi W., Diebler M.-F., Weihe E., Rand J.B., Eiden L.E., Bonner T.I., Usdin T.B.
J. Biol. Chem. 269:21929-21932(1994) [PubMed: 8071310] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Pheochromocytoma.
[3]"A unique gene organization for two cholinergic markers, choline acetyltransferase and a putative vesicular transporter of acetylcholine."
Bejanin S., Cervini R., Mallet J., Berrard S.
J. Biol. Chem. 269:21944-21947(1994) [PubMed: 8071313] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Spinal cord.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U09838 mRNA. Translation: AAA50831.1.
U09211 mRNA. Translation: AAA20498.1.
X80395 Genomic DNA. Translation: CAA56604.1.
IPIIPI00326574.
RefSeqNP_113851.1.
UniGeneRn.9987

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ62666.

PTM databases

PhosphoSiteQ62666.

Genome annotation databases

EnsemblENSRNOT00000034178; ENSRNOP00000034949; ENSRNOG00000025008; Rattus norvegicus. [Genome view]
GeneID60422.
KEGGrno:60422.
UCSCNM_031663. rat.

Organism-specific databases

CTD60422.
RGD62072. Slc18a3.

Phylogenomic databases

HOVERGENQ62666.

Gene expression databases

ArrayExpressQ62666.
GenevestigatorQ62666.
GermOnlineENSRNOG00000025008. Rattus norvegicus.

Family and domain databases

InterProIPR011701. MFS_1.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio612138.

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Entry information

Entry nameVACHT_RAT
AccessionPrimary (citable) accession number: Q62666
Secondary accession number(s): Q64351
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: September 19, 2003
Last modified: October 13, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents