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Protein

Vesicular acetylcholine transporter

Gene

Slc18a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in acetylcholine transport into synaptic vesicles.1 Publication

GO - Molecular functioni

  • acetylcholine binding Source: RGD
  • acetylcholine transmembrane transporter activity Source: UniProtKB
  • monoamine transmembrane transporter activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular acetylcholine transporter
Short name:
VAChT
Short name:
rVAT
Alternative name(s):
Solute carrier family 18 member 3
Gene namesi
Name:Slc18a3
Synonyms:Vacht, Vat
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi62072. Slc18a3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 125Lumenal, vesicleSequence analysisAdd BLAST71
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Topological domaini147 – 152CytoplasmicSequence analysis6
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 182Lumenal, vesicleSequence analysis9
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 213CytoplasmicSequence analysis10
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Topological domaini235 – 242Lumenal, vesicleSequence analysis8
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 289CytoplasmicSequence analysisAdd BLAST26
Transmembranei290 – 310HelicalSequence analysisAdd BLAST21
Topological domaini311 – 325Lumenal, vesicleSequence analysisAdd BLAST15
Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
Topological domaini347 – 356CytoplasmicSequence analysis10
Transmembranei357 – 377HelicalSequence analysisAdd BLAST21
Topological domaini378 – 388Lumenal, vesicleSequence analysisAdd BLAST11
Transmembranei389 – 409HelicalSequence analysisAdd BLAST21
Topological domaini410 – 422CytoplasmicSequence analysisAdd BLAST13
Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
Topological domaini444 – 447Lumenal, vesicleSequence analysis4
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 530CytoplasmicSequence analysisAdd BLAST62

GO - Cellular componenti

  • AP-1 adaptor complex Source: RGD
  • AP-2 adaptor complex Source: RGD
  • axon Source: RGD
  • axon terminus Source: RGD
  • clathrin-sculpted acetylcholine transport vesicle membrane Source: UniProtKB
  • cytoplasmic vesicle Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • synaptic vesicle Source: RGD
  • synaptic vesicle membrane Source: GO_Central
  • terminal bouton Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2125.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275201 – 530Vesicular acetylcholine transporterAdd BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...)Sequence analysis1
Glycosylationi96N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

PTM databases

iPTMnetiQ62666.
PhosphoSitePlusiQ62666.

Expressioni

Tissue specificityi

High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus.3 Publications

Interactioni

Subunit structurei

Interacts with SEC14L1.By similarity

Chemistry databases

BindingDBiQ62666.

Structurei

3D structure databases

ProteinModelPortaliQ62666.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni471 – 530Mediates interaction with SEC14L1By similarityAdd BLAST60

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ62666.
KOiK14636.
PhylomeDBiQ62666.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY
60 70 80 90 100
MVIVPIVPDY IAHMRGGSEG PTLVSEVWEP TLPPPTLANA SAYLANTSAS
110 120 130 140 150
PTAAGSARSI LRPRYPTESE DVKIGVLFAS KAILQLLVNP LSGPFIDRMS
160 170 180 190 200
YDVPLLIGLG VMFASTVMFA FAEDYATLFA ARSLQGLGSA FADTSGIAMI
210 220 230 240 250
ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG KRVPFLVLAA
260 270 280 290 300
VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT
310 320 330 340 350
CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA
360 370 380 390 400
RYPHLQWLYG ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA
410 420 430 440 450
LLPTLAFLVD VRHVSVYGSV YAIADISYSV AYALGPIVAG HIVHSLGFEQ
460 470 480 490 500
LSLGMGLANL LYAPVLLLLR NVGLLTRSRS ERDVLLDEPP QGLYDAVRLR
510 520 530
EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS
Length:530
Mass (Da):56,537
Last modified:September 19, 2003 - v2
Checksum:i07720DA749527438
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92A → T in AAA50831 (PubMed:7938002).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09838 mRNA. Translation: AAA50831.1.
U09211 mRNA. Translation: AAA20498.1.
X80395 Genomic DNA. Translation: CAA56604.1.
RefSeqiNP_113851.1. NM_031663.2.
UniGeneiRn.9987.

Genome annotation databases

GeneIDi60422.
KEGGirno:60422.
UCSCiRGD:62072. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09838 mRNA. Translation: AAA50831.1.
U09211 mRNA. Translation: AAA20498.1.
X80395 Genomic DNA. Translation: CAA56604.1.
RefSeqiNP_113851.1. NM_031663.2.
UniGeneiRn.9987.

3D structure databases

ProteinModelPortaliQ62666.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiQ62666.
ChEMBLiCHEMBL2125.

PTM databases

iPTMnetiQ62666.
PhosphoSitePlusiQ62666.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi60422.
KEGGirno:60422.
UCSCiRGD:62072. rat.

Organism-specific databases

CTDi6572.
RGDi62072. Slc18a3.

Phylogenomic databases

HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ62666.
KOiK14636.
PhylomeDBiQ62666.

Miscellaneous databases

PROiQ62666.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVACHT_RAT
AccessioniPrimary (citable) accession number: Q62666
Secondary accession number(s): Q64351
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: September 19, 2003
Last modified: November 2, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.