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Protein

Vesicular acetylcholine transporter

Gene

Slc18a3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in acetylcholine transport into synaptic vesicles.1 Publication

GO - Molecular functioni

  • acetylcholine binding Source: RGD
  • acetylcholine transmembrane transporter activity Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular acetylcholine transporter
Short name:
VAChT
Short name:
rVAT
Alternative name(s):
Solute carrier family 18 member 3
Gene namesi
Name:Slc18a3
Synonyms:Vacht, Vat
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi62072. Slc18a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence analysisAdd
BLAST
Transmembranei34 – 5421HelicalSequence analysisAdd
BLAST
Topological domaini55 – 12571Lumenal, vesicleSequence analysisAdd
BLAST
Transmembranei126 – 14621HelicalSequence analysisAdd
BLAST
Topological domaini147 – 1526CytoplasmicSequence analysis
Transmembranei153 – 17321HelicalSequence analysisAdd
BLAST
Topological domaini174 – 1829Lumenal, vesicleSequence analysis
Transmembranei183 – 20321HelicalSequence analysisAdd
BLAST
Topological domaini204 – 21310CytoplasmicSequence analysis
Transmembranei214 – 23421HelicalSequence analysisAdd
BLAST
Topological domaini235 – 2428Lumenal, vesicleSequence analysis
Transmembranei243 – 26321HelicalSequence analysisAdd
BLAST
Topological domaini264 – 28926CytoplasmicSequence analysisAdd
BLAST
Transmembranei290 – 31021HelicalSequence analysisAdd
BLAST
Topological domaini311 – 32515Lumenal, vesicleSequence analysisAdd
BLAST
Transmembranei326 – 34621HelicalSequence analysisAdd
BLAST
Topological domaini347 – 35610CytoplasmicSequence analysis
Transmembranei357 – 37721HelicalSequence analysisAdd
BLAST
Topological domaini378 – 38811Lumenal, vesicleSequence analysisAdd
BLAST
Transmembranei389 – 40921HelicalSequence analysisAdd
BLAST
Topological domaini410 – 42213CytoplasmicSequence analysisAdd
BLAST
Transmembranei423 – 44321HelicalSequence analysisAdd
BLAST
Topological domaini444 – 4474Lumenal, vesicleSequence analysis
Transmembranei448 – 46821HelicalSequence analysisAdd
BLAST
Topological domaini469 – 53062CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • AP-1 adaptor complex Source: RGD
  • AP-2 adaptor complex Source: RGD
  • axon Source: RGD
  • axon terminus Source: RGD
  • clathrin-sculpted acetylcholine transport vesicle membrane Source: UniProtKB
  • cytoplasmic vesicle Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • synaptic vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2125.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Vesicular acetylcholine transporterPRO_0000127520Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence analysis
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

PTM databases

iPTMnetiQ62666.
PhosphoSiteiQ62666.

Expressioni

Tissue specificityi

High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus.3 Publications

Interactioni

Subunit structurei

Interacts with SEC14L1.By similarity

Chemistry

BindingDBiQ62666.

Structurei

3D structure databases

ProteinModelPortaliQ62666.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni471 – 53060Mediates interaction with SEC14L1By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ62666.
KOiK14636.
PhylomeDBiQ62666.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY
60 70 80 90 100
MVIVPIVPDY IAHMRGGSEG PTLVSEVWEP TLPPPTLANA SAYLANTSAS
110 120 130 140 150
PTAAGSARSI LRPRYPTESE DVKIGVLFAS KAILQLLVNP LSGPFIDRMS
160 170 180 190 200
YDVPLLIGLG VMFASTVMFA FAEDYATLFA ARSLQGLGSA FADTSGIAMI
210 220 230 240 250
ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG KRVPFLVLAA
260 270 280 290 300
VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT
310 320 330 340 350
CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA
360 370 380 390 400
RYPHLQWLYG ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA
410 420 430 440 450
LLPTLAFLVD VRHVSVYGSV YAIADISYSV AYALGPIVAG HIVHSLGFEQ
460 470 480 490 500
LSLGMGLANL LYAPVLLLLR NVGLLTRSRS ERDVLLDEPP QGLYDAVRLR
510 520 530
EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS
Length:530
Mass (Da):56,537
Last modified:September 19, 2003 - v2
Checksum:i07720DA749527438
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921A → T in AAA50831 (PubMed:7938002).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09838 mRNA. Translation: AAA50831.1.
U09211 mRNA. Translation: AAA20498.1.
X80395 Genomic DNA. Translation: CAA56604.1.
RefSeqiNP_113851.1. NM_031663.2.
UniGeneiRn.9987.

Genome annotation databases

GeneIDi60422.
KEGGirno:60422.
UCSCiRGD:62072. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09838 mRNA. Translation: AAA50831.1.
U09211 mRNA. Translation: AAA20498.1.
X80395 Genomic DNA. Translation: CAA56604.1.
RefSeqiNP_113851.1. NM_031663.2.
UniGeneiRn.9987.

3D structure databases

ProteinModelPortaliQ62666.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ62666.
ChEMBLiCHEMBL2125.

PTM databases

iPTMnetiQ62666.
PhosphoSiteiQ62666.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi60422.
KEGGirno:60422.
UCSCiRGD:62072. rat.

Organism-specific databases

CTDi6572.
RGDi62072. Slc18a3.

Phylogenomic databases

HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ62666.
KOiK14636.
PhylomeDBiQ62666.

Miscellaneous databases

PROiQ62666.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain stem.
  2. "Functional identification of a vesicular acetylcholine transporter and its expression from a 'cholinergic' gene locus."
    Erickson J.D., Varoqui H., Schafer M.K.-H., Modi W., Diebler M.-F., Weihe E., Rand J.B., Eiden L.E., Bonner T.I., Usdin T.B.
    J. Biol. Chem. 269:21929-21932(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Pheochromocytoma.
  3. "A unique gene organization for two cholinergic markers, choline acetyltransferase and a putative vesicular transporter of acetylcholine."
    Bejanin S., Cervini R., Mallet J., Berrard S.
    J. Biol. Chem. 269:21944-21947(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.

Entry informationi

Entry nameiVACHT_RAT
AccessioniPrimary (citable) accession number: Q62666
Secondary accession number(s): Q64351
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: September 19, 2003
Last modified: July 6, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.