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Protein

Galectin-8

Gene

Lgals8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy. Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture. Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52. Required to restrict infection of bacterial invasion such as S.typhimurium. Also required to restrict infection of Picornaviridae viruses. Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei58Critical for binding to sialylated and sulfated oligosaccharidesBy similarity1
Binding sitei68CarbohydrateBy similarity1
Binding sitei78CarbohydrateBy similarity1
Binding sitei88CarbohydrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy
LigandLectin

Names & Taxonomyi

Protein namesi
Recommended name:
Galectin-8
Short name:
Gal-8
Alternative name(s):
30 kDa S-type lectin
RL-30
Gene namesi
Name:Lgals8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621272. Lgals8.

Subcellular locationi

  • Cytoplasmic vesicle By similarity
  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000769451 – 316Galectin-8Add BLAST316

Proteomic databases

PaxDbiQ62665.
PRIDEiQ62665.

Expressioni

Tissue specificityi

Expressed in liver, kidney, cardiac muscle, lung, and brain.

Developmental stagei

Very low levels in whole embryos, high levels in adult tissues.

Interactioni

Subunit structurei

Homodimer. Interacts with CALCOCO2/NDP52.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040412.

Structurei

3D structure databases

ProteinModelPortaliQ62665.
SMRiQ62665.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 151Galectin 1PROSITE-ProRule annotationAdd BLAST134
Domaini186 – 316Galectin 2PROSITE-ProRule annotationAdd BLAST131

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni248 – 254Beta-galactoside bindingBy similarity7

Domaini

Contains two homologous but distinct carbohydrate-binding domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
HOGENOMiHOG000290194.
HOVERGENiHBG002412.
InParanoidiQ62665.
PhylomeDBiQ62665.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR030638. Galectin_8.
IPR001079. Galectin_CRD.
PANTHERiPTHR11346:SF125. PTHR11346:SF125. 1 hit.
PfamiView protein in Pfam
PF00337. Gal-bind_lectin. 2 hits.
SMARTiView protein in SMART
SM00908. Gal-bind_lectin. 2 hits.
SM00276. GLECT. 2 hits.
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiView protein in PROSITE
PS51304. GALECTIN. 2 hits.

Sequencei

Sequence statusi: Complete.

Q62665-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSLSNLQNI IYNPTIPYVS TITEQLKPGS LIVIRGHVPK DSERFQVDFQ
60 70 80 90 100
HGNSLKPRAD VAFHFNPRFK RSNCIVCNTL TNEKWGWEEI THDMPFRKEK
110 120 130 140 150
SFEIVIMVLK NKFHVAVNGK HILLYAHRIN PEKIDTLGIF GKVNIHSIGF
160 170 180 190 200
RFSSDLQSME TSTLGLTQIS KENIQKSGKL HLSLPFEARL NASMGPGRTV
210 220 230 240 250
VVKGEVNTNA TSFNVDLVAG RSRDIALHLN PRLNVKAFVR NSFLQDAWGE
260 270 280 290 300
EERNITCFPF SSGMYFEMII YCDVREFKVA VNGVHSLEYK HRFKDLSSID
310
TLAVDGDIRL LDVRSW
Length:316
Mass (Da):36,038
Last modified:November 1, 1996 - v1
Checksum:iC04B766CFE913D59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09824 mRNA. Translation: AAA66359.1.
PIRiA55975.
UniGeneiRn.10069.

Genome annotation databases

UCSCiRGD:621272. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09824 mRNA. Translation: AAA66359.1.
PIRiA55975.
UniGeneiRn.10069.

3D structure databases

ProteinModelPortaliQ62665.
SMRiQ62665.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040412.

Proteomic databases

PaxDbiQ62665.
PRIDEiQ62665.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621272. rat.

Organism-specific databases

RGDi621272. Lgals8.

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
HOGENOMiHOG000290194.
HOVERGENiHBG002412.
InParanoidiQ62665.
PhylomeDBiQ62665.

Miscellaneous databases

PROiPR:Q62665.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR030638. Galectin_8.
IPR001079. Galectin_CRD.
PANTHERiPTHR11346:SF125. PTHR11346:SF125. 1 hit.
PfamiView protein in Pfam
PF00337. Gal-bind_lectin. 2 hits.
SMARTiView protein in SMART
SM00908. Gal-bind_lectin. 2 hits.
SM00276. GLECT. 2 hits.
SUPFAMiSSF49899. SSF49899. 2 hits.
PROSITEiView protein in PROSITE
PS51304. GALECTIN. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiLEG8_RAT
AccessioniPrimary (citable) accession number: Q62665
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 12, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.