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Q62662

- FRK_RAT

UniProt

Q62662 - FRK_RAT

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Protein

Tyrosine-protein kinase FRK

Gene

Frk

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei263 – 2631ATPPROSITE-ProRule annotation
Active sitei355 – 3551Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi241 – 2499ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. protein tyrosine kinase activity Source: RGD

GO - Biological processi

  1. cell differentiation Source: RGD
  2. peptidyl-tyrosine phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase FRK (EC:2.7.10.2)
Alternative name(s):
FYN-related kinase
Gastrointestinal-associated kinase
Short name:
GASK
Gastrointestinal-associated tyrosine kinase
Short name:
GTK
Gene namesi
Name:Frk
Synonyms:Gask
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi621423. Frk.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Predominantly found in the nucleus, with a small fraction found in the cell periphery.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. nucleus Source: UniProtKB-KW
  4. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Tyrosine-protein kinase FRKPRO_0000260827Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei388 – 3881Phosphotyrosine; by autocatalysisBy similarity

Keywords - PTMi

Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ62662.
PRIDEiQ62662.

PTM databases

PhosphoSiteiQ62662.

Expressioni

Tissue specificityi

Highly expressed in stomach, small intestine and colon. Concentrated in the brush border membranes of epithelial cells, throughout the maturation axis of the adult small intestine.2 Publications

Gene expression databases

GenevestigatoriQ62662.

Interactioni

Subunit structurei

Interacts (via the SH3-domain) with PTEN. Interacts with RB1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000653.

Structurei

3D structure databases

ProteinModelPortaliQ62662.
SMRiQ62662. Positions 43-495.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 11170SH3PROSITE-ProRule annotationAdd
BLAST
Domaini117 – 20993SH2PROSITE-ProRule annotationAdd
BLAST
Domaini235 – 492258Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118938.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiQ62662.
KOiK08892.
OMAiVSHYTKT.
OrthoDBiEOG7GTT2V.
PhylomeDBiQ62662.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62662-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSVCMRLWA YLQPFLPCWS QEADKSVVIE NPGAYCPPEA NRSQGQYFVA
60 70 80 90 100
LFDYEARTAE DLSFHAGDKL QVLDTSHEGW WLARHLEKKG PGLGQQLQGY
110 120 130 140 150
IPSNYVAEDR SLQAEPWFFG AIKRADAEKQ LLYSENQTGA FLIRESESQK
160 170 180 190 200
GDFSLSVLDE GVVKHYRIRR LDEGGFFLTR RKTFSTLNEF VNYYTTTSDG
210 220 230 240 250
LCVKLEKPCL KIQVPTPFDL SYKTVDQWEI DRNSIQLLKR LGSGQFGEVW
260 270 280 290 300
EGLWNNTTPV AVKTLKPGSM DPNDFLREAQ IMKSLRHPKL IQLYAVCTLE
310 320 330 340 350
DPIYIITELM RHGSLQEYLQ NDGGSKIRLT QQVDMAAQVA SGMAYLESQN
360 370 380 390 400
YIHRDLAARN VLVGEHNIYK VADFGLARVF KVDNEDIYES KHEIKLPVKW
410 420 430 440 450
TAPEAIRTNK FSIKSDVWSF GILLYEIITY GKMPYSGMTG AQVIHMLGQN
460 470 480 490 500
YRLPQPSNCP EQFYSIMMEC WNVEPKQRPT FETLHWKLED YFEPDSSYSD

TNNFIN
Length:506
Mass (Da):58,166
Last modified:January 23, 2007 - v3
Checksum:i69F471DE4F974D82
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09583 mRNA. Translation: AAC52725.1.
PIRiS43515.
RefSeqiNP_077344.1. NM_024368.1.
XP_006256579.1. XM_006256517.2.
UniGeneiRn.10556.

Genome annotation databases

EnsembliENSRNOT00000000653; ENSRNOP00000000653; ENSRNOG00000000543.
GeneIDi79209.
KEGGirno:79209.
UCSCiRGD:621423. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09583 mRNA. Translation: AAC52725.1 .
PIRi S43515.
RefSeqi NP_077344.1. NM_024368.1.
XP_006256579.1. XM_006256517.2.
UniGenei Rn.10556.

3D structure databases

ProteinModelPortali Q62662.
SMRi Q62662. Positions 43-495.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000000653.

PTM databases

PhosphoSitei Q62662.

Proteomic databases

PaxDbi Q62662.
PRIDEi Q62662.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000000653 ; ENSRNOP00000000653 ; ENSRNOG00000000543 .
GeneIDi 79209.
KEGGi rno:79209.
UCSCi RGD:621423. rat.

Organism-specific databases

CTDi 2444.
RGDi 621423. Frk.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000118938.
HOGENOMi HOG000233858.
HOVERGENi HBG008761.
InParanoidi Q62662.
KOi K08892.
OMAi VSHYTKT.
OrthoDBi EOG7GTT2V.
PhylomeDBi Q62662.

Miscellaneous databases

NextBioi 614596.
PROi Q62662.

Gene expression databases

Genevestigatori Q62662.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The apical membranes of maturing gut columnar epithelial cells contain the enzymatically active form of a newly identified fyn-related tyrosine kinase."
    Sunitha I., Avigan M.I.
    Oncogene 13:547-559(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Small intestine.
  2. "A newly identified tyrosine kinase is preferentially expressed in the gastrointestinal tract."
    Sunitha I., Avigan M.I.
    Biochim. Biophys. Acta 1221:348-352(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Small intestine.

Entry informationi

Entry nameiFRK_RAT
AccessioniPrimary (citable) accession number: Q62662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3