Tyrosine-protein kinase FRK - Rattus norvegicus (Rat)

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Q62662 (FRK_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase FRK
EC=2.7.10.2

Alternative name(s):
FYN-related kinase
Gastrointestinal-associated kinase
Short name=GASK
Gastrointestinal-associated tyrosine kinase
Short name=GTK

Gene names

Name:	Frk
Synonyms:	Gask

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts (via the SH3-domain) with PTEN. Interacts with RB1 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Predominantly found in the nucleus, with a small fraction found in the cell periphery By similarity.
Tissue specificity	Highly expressed in stomach, small intestine and colon. Concentrated in the brush border membranes of epithelial cells, throughout the maturation axis of the adult small intestine. Ref.1 Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Disease	Tumor suppressor
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from expression pattern Ref.1. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay Ref.1. Source: RGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 506

506

Tyrosine-protein kinase FRK

PRO_0000260827

Regions

Domain

42 – 111

SH3

Domain

117 – 209

SH2

Domain

235 – 492

258

Protein kinase

Nucleotide binding

241 – 249

ATP By similarity

Sites

Active site

355

Proton acceptor By similarity

Binding site

263

ATP By similarity

Amino acid modifications

Modified residue

388

Phosphotyrosine; by autocatalysis By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q62662 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 69F471DE4F974D82
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FASTA

506

58,166

        10         20         30         40         50         60 
MGSVCMRLWA YLQPFLPCWS QEADKSVVIE NPGAYCPPEA NRSQGQYFVA LFDYEARTAE 

        70         80         90        100        110        120 
DLSFHAGDKL QVLDTSHEGW WLARHLEKKG PGLGQQLQGY IPSNYVAEDR SLQAEPWFFG 

       130        140        150        160        170        180 
AIKRADAEKQ LLYSENQTGA FLIRESESQK GDFSLSVLDE GVVKHYRIRR LDEGGFFLTR 

       190        200        210        220        230        240 
RKTFSTLNEF VNYYTTTSDG LCVKLEKPCL KIQVPTPFDL SYKTVDQWEI DRNSIQLLKR 

       250        260        270        280        290        300 
LGSGQFGEVW EGLWNNTTPV AVKTLKPGSM DPNDFLREAQ IMKSLRHPKL IQLYAVCTLE 

       310        320        330        340        350        360 
DPIYIITELM RHGSLQEYLQ NDGGSKIRLT QQVDMAAQVA SGMAYLESQN YIHRDLAARN 

       370        380        390        400        410        420 
VLVGEHNIYK VADFGLARVF KVDNEDIYES KHEIKLPVKW TAPEAIRTNK FSIKSDVWSF 

       430        440        450        460        470        480 
GILLYEIITY GKMPYSGMTG AQVIHMLGQN YRLPQPSNCP EQFYSIMMEC WNVEPKQRPT 

       490        500 
FETLHWKLED YFEPDSSYSD TNNFIN

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References

[1]	"The apical membranes of maturing gut columnar epithelial cells contain the enzymatically active form of a newly identified fyn-related tyrosine kinase." Sunitha I., Avigan M.I. Oncogene 13:547-559(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Small intestine.
[2]	"A newly identified tyrosine kinase is preferentially expressed in the gastrointestinal tract." Sunitha I., Avigan M.I. Biochim. Biophys. Acta 1221:348-352(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Small intestine.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09583 mRNA. Translation: AAC52725.1.
IPI	IPI00207139.
PIR	S43515.
RefSeq	NP_077344.1. NM_024368.1.
UniGene	Rn.10556.
3D structure databases
HSSP	HSSP built from PDB template 1YOJ based on UniProtKB P12931.
ProteinModelPortal	Q62662.
SMR	Q62662. Positions 43-495.
ModBase	Search...
PTM databases
PhosphoSite	Q62662.
Proteomic databases
PaxDb	Q62662.
PRIDE	Q62662.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000000653; ENSRNOP00000000653; ENSRNOG00000000543.
GeneID	79209.
KEGG	rno:79209.
UCSC	RGD:621423. rat.
Organism-specific databases
CTD	2444.
RGD	621423. Frk.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00620000087702.
HOGENOM	HOG000233858.
HOVERGEN	HBG008761.
InParanoid	Q62662.
KO	K08892.
OrthoDB	EOG4FJ88S.
Gene expression databases
ArrayExpress	Q62662.
Genevestigator	Q62662.
GermOnline	ENSRNOG00000000543. Rattus norvegicus.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00109. TYRKINASE.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	614596.

Entry information

Entry name

FRK_RAT

Accession

Primary (citable) accession number: Q62662

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents