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Q62662 (FRK_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein kinase FRK

EC=2.7.10.2
Alternative name(s):
FYN-related kinase
Gastrointestinal-associated kinase
Short name=GASK
Gastrointestinal-associated tyrosine kinase
Short name=GTK
Gene names
Name:Frk
Synonyms:Gask
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor By similarity.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts (via the SH3-domain) with PTEN. Interacts with RB1 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Predominantly found in the nucleus, with a small fraction found in the cell periphery By similarity.

Tissue specificity

Highly expressed in stomach, small intestine and colon. Concentrated in the brush border membranes of epithelial cells, throughout the maturation axis of the adult small intestine. Ref.1 Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Contains 1 SH3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506Tyrosine-protein kinase FRK
PRO_0000260827

Regions

Domain42 – 11170SH3
Domain117 – 20993SH2
Domain235 – 492258Protein kinase
Nucleotide binding241 – 2499ATP By similarity

Sites

Active site3551Proton acceptor By similarity
Binding site2631ATP By similarity

Amino acid modifications

Modified residue3881Phosphotyrosine; by autocatalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q62662 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 69F471DE4F974D82

FASTA50658,166
        10         20         30         40         50         60 
MGSVCMRLWA YLQPFLPCWS QEADKSVVIE NPGAYCPPEA NRSQGQYFVA LFDYEARTAE 

        70         80         90        100        110        120 
DLSFHAGDKL QVLDTSHEGW WLARHLEKKG PGLGQQLQGY IPSNYVAEDR SLQAEPWFFG 

       130        140        150        160        170        180 
AIKRADAEKQ LLYSENQTGA FLIRESESQK GDFSLSVLDE GVVKHYRIRR LDEGGFFLTR 

       190        200        210        220        230        240 
RKTFSTLNEF VNYYTTTSDG LCVKLEKPCL KIQVPTPFDL SYKTVDQWEI DRNSIQLLKR 

       250        260        270        280        290        300 
LGSGQFGEVW EGLWNNTTPV AVKTLKPGSM DPNDFLREAQ IMKSLRHPKL IQLYAVCTLE 

       310        320        330        340        350        360 
DPIYIITELM RHGSLQEYLQ NDGGSKIRLT QQVDMAAQVA SGMAYLESQN YIHRDLAARN 

       370        380        390        400        410        420 
VLVGEHNIYK VADFGLARVF KVDNEDIYES KHEIKLPVKW TAPEAIRTNK FSIKSDVWSF 

       430        440        450        460        470        480 
GILLYEIITY GKMPYSGMTG AQVIHMLGQN YRLPQPSNCP EQFYSIMMEC WNVEPKQRPT 

       490        500 
FETLHWKLED YFEPDSSYSD TNNFIN 

« Hide

References

[1]"The apical membranes of maturing gut columnar epithelial cells contain the enzymatically active form of a newly identified fyn-related tyrosine kinase."
Sunitha I., Avigan M.I.
Oncogene 13:547-559(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Small intestine.
[2]"A newly identified tyrosine kinase is preferentially expressed in the gastrointestinal tract."
Sunitha I., Avigan M.I.
Biochim. Biophys. Acta 1221:348-352(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Small intestine.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09583 mRNA. Translation: AAC52725.1.
PIRS43515.
RefSeqNP_077344.1. NM_024368.1.
XP_006256579.1. XM_006256517.1.
UniGeneRn.10556.

3D structure databases

ProteinModelPortalQ62662.
SMRQ62662. Positions 43-495.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000000653.

PTM databases

PhosphoSiteQ62662.

Proteomic databases

PaxDbQ62662.
PRIDEQ62662.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000000653; ENSRNOP00000000653; ENSRNOG00000000543.
GeneID79209.
KEGGrno:79209.
UCSCRGD:621423. rat.

Organism-specific databases

CTD2444.
RGD621423. Frk.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00640000091347.
HOGENOMHOG000233858.
HOVERGENHBG008761.
InParanoidQ62662.
KOK08892.
OMAVSHYTKT.
OrthoDBEOG7GTT2V.
PhylomeDBQ62662.

Gene expression databases

GenevestigatorQ62662.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio614596.
PROQ62662.

Entry information

Entry nameFRK_RAT
AccessionPrimary (citable) accession number: Q62662
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families