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Q62662

- FRK_RAT

UniProt

Q62662 - FRK_RAT

Protein

Tyrosine-protein kinase FRK

Gene

Frk

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor By similarity.By similarity

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei263 – 2631ATPPROSITE-ProRule annotation
    Active sitei355 – 3551Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi241 – 2499ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein tyrosine kinase activity Source: RGD

    GO - Biological processi

    1. cell differentiation Source: RGD
    2. peptidyl-tyrosine phosphorylation Source: GOC

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase FRK (EC:2.7.10.2)
    Alternative name(s):
    FYN-related kinase
    Gastrointestinal-associated kinase
    Short name:
    GASK
    Gastrointestinal-associated tyrosine kinase
    Short name:
    GTK
    Gene namesi
    Name:Frk
    Synonyms:Gask
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 20

    Organism-specific databases

    RGDi621423. Frk.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Predominantly found in the nucleus, with a small fraction found in the cell periphery.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 506506Tyrosine-protein kinase FRKPRO_0000260827Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei388 – 3881Phosphotyrosine; by autocatalysisBy similarity

    Keywords - PTMi

    Lipoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ62662.
    PRIDEiQ62662.

    PTM databases

    PhosphoSiteiQ62662.

    Expressioni

    Tissue specificityi

    Highly expressed in stomach, small intestine and colon. Concentrated in the brush border membranes of epithelial cells, throughout the maturation axis of the adult small intestine.2 Publications

    Gene expression databases

    GenevestigatoriQ62662.

    Interactioni

    Subunit structurei

    Interacts (via the SH3-domain) with PTEN. Interacts with RB1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000000653.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62662.
    SMRiQ62662. Positions 43-495.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 11170SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini117 – 20993SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini235 – 492258Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00640000091347.
    HOGENOMiHOG000233858.
    HOVERGENiHBG008761.
    InParanoidiQ62662.
    KOiK08892.
    OMAiVSHYTKT.
    OrthoDBiEOG7GTT2V.
    PhylomeDBiQ62662.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q62662-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSVCMRLWA YLQPFLPCWS QEADKSVVIE NPGAYCPPEA NRSQGQYFVA    50
    LFDYEARTAE DLSFHAGDKL QVLDTSHEGW WLARHLEKKG PGLGQQLQGY 100
    IPSNYVAEDR SLQAEPWFFG AIKRADAEKQ LLYSENQTGA FLIRESESQK 150
    GDFSLSVLDE GVVKHYRIRR LDEGGFFLTR RKTFSTLNEF VNYYTTTSDG 200
    LCVKLEKPCL KIQVPTPFDL SYKTVDQWEI DRNSIQLLKR LGSGQFGEVW 250
    EGLWNNTTPV AVKTLKPGSM DPNDFLREAQ IMKSLRHPKL IQLYAVCTLE 300
    DPIYIITELM RHGSLQEYLQ NDGGSKIRLT QQVDMAAQVA SGMAYLESQN 350
    YIHRDLAARN VLVGEHNIYK VADFGLARVF KVDNEDIYES KHEIKLPVKW 400
    TAPEAIRTNK FSIKSDVWSF GILLYEIITY GKMPYSGMTG AQVIHMLGQN 450
    YRLPQPSNCP EQFYSIMMEC WNVEPKQRPT FETLHWKLED YFEPDSSYSD 500
    TNNFIN 506
    Length:506
    Mass (Da):58,166
    Last modified:January 23, 2007 - v3
    Checksum:i69F471DE4F974D82
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09583 mRNA. Translation: AAC52725.1.
    PIRiS43515.
    RefSeqiNP_077344.1. NM_024368.1.
    XP_006256579.1. XM_006256517.1.
    UniGeneiRn.10556.

    Genome annotation databases

    EnsembliENSRNOT00000000653; ENSRNOP00000000653; ENSRNOG00000000543.
    GeneIDi79209.
    KEGGirno:79209.
    UCSCiRGD:621423. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09583 mRNA. Translation: AAC52725.1 .
    PIRi S43515.
    RefSeqi NP_077344.1. NM_024368.1.
    XP_006256579.1. XM_006256517.1.
    UniGenei Rn.10556.

    3D structure databases

    ProteinModelPortali Q62662.
    SMRi Q62662. Positions 43-495.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000000653.

    PTM databases

    PhosphoSitei Q62662.

    Proteomic databases

    PaxDbi Q62662.
    PRIDEi Q62662.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000000653 ; ENSRNOP00000000653 ; ENSRNOG00000000543 .
    GeneIDi 79209.
    KEGGi rno:79209.
    UCSCi RGD:621423. rat.

    Organism-specific databases

    CTDi 2444.
    RGDi 621423. Frk.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00640000091347.
    HOGENOMi HOG000233858.
    HOVERGENi HBG008761.
    InParanoidi Q62662.
    KOi K08892.
    OMAi VSHYTKT.
    OrthoDBi EOG7GTT2V.
    PhylomeDBi Q62662.

    Miscellaneous databases

    NextBioi 614596.
    PROi Q62662.

    Gene expression databases

    Genevestigatori Q62662.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The apical membranes of maturing gut columnar epithelial cells contain the enzymatically active form of a newly identified fyn-related tyrosine kinase."
      Sunitha I., Avigan M.I.
      Oncogene 13:547-559(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Small intestine.
    2. "A newly identified tyrosine kinase is preferentially expressed in the gastrointestinal tract."
      Sunitha I., Avigan M.I.
      Biochim. Biophys. Acta 1221:348-352(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Small intestine.

    Entry informationi

    Entry nameiFRK_RAT
    AccessioniPrimary (citable) accession number: Q62662
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 102 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3