SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q62656

- PTPRZ_RAT

UniProt

Q62656 - PTPRZ_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Receptor-type tyrosine-protein phosphatase zeta

Gene
Ptprz1, Ptprz, Ptpz
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. Required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. May play a role in protecting oligondendrocytes against apoptosis. May play a role in the establishment of contextual memory, probably via the dephosphorylation of proteins that are part of important signaling cascades By similarity.
Isoform 3 (phosphacan), previously designated 3F8 chondroitin sulfate proteoglycan or 3H1 keratan sulfate proteoglycan depending on the glycosylation status, is a soluble nervous tissue-specific proteoglycan. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1902 – 19021Substrate By similarity
Active sitei1934 – 19341Phosphocysteine intermediate By similarity
Binding sitei1978 – 19781Substrate By similarity
Sitei2224 – 22241Ancestral active site

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. learning or memory Source: UniProtKB
  2. oligodendrocyte differentiation Source: UniProtKB
  3. peptidyl-tyrosine dephosphorylation Source: UniProtKB
  4. regulation of oligodendrocyte progenitor proliferation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase zeta (EC:3.1.3.48)
Short name:
R-PTP-zeta
Alternative name(s):
3F8 chondroitin sulfate proteoglycan
3H1 keratan sulfate proteoglycan
Phosphacan
Gene namesi
Name:Ptprz1
Synonyms:Ptprz, Ptpz
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3455. Ptprz1.

Subcellular locationi

Isoform 1 : Cell membrane; Single-pass type I membrane protein. Secreted By similarity
Note: A secreted form is apparently generated by shedding of the extracellular domain By similarity.
Isoform 2 : Secreted Reviewed prediction

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 16371613Extracellular Reviewed predictionAdd
BLAST
Transmembranei1638 – 166326Helical; Reviewed predictionAdd
BLAST
Topological domaini1664 – 2316653Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Add
BLAST
Chaini25 – 23162292Receptor-type tyrosine-protein phosphatase zetaPRO_0000025469Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 240 By similarity
Glycosylationi105 – 1051N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi133 ↔ 264 By similarity
Glycosylationi134 – 1341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi223 – 2231N-linked (GlcNAc...) Reviewed prediction
Glycosylationi232 – 2321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi324 – 3241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi381 – 3811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi497 – 4971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi552 – 5521N-linked (GlcNAc...) Reviewed prediction
Glycosylationi595 – 5951O-linked (Xyl...) (chondroitin sulfate) Reviewed prediction
Glycosylationi610 – 6101N-linked (GlcNAc...) Reviewed prediction
Glycosylationi645 – 6451O-linked (Xyl...) (chondroitin sulfate) Reviewed prediction
Glycosylationi685 – 6851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi786 – 7861N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1005 – 10051O-linked (Xyl...) (chondroitin sulfate) Reviewed prediction
Glycosylationi1025 – 10251N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1058 – 10581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1463 – 14631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1550 – 15501O-linked (Xyl...) (chondroitin sulfate) Reviewed prediction
Glycosylationi1552 – 15521O-linked (Xyl...) (chondroitin sulfate) Reviewed prediction
Glycosylationi1563 – 15631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1611 – 16111N-linked (GlcNAc...) Reviewed prediction
Glycosylationi1619 – 16191N-linked (GlcNAc...) Reviewed prediction
Modified residuei2056 – 20561Phosphoserine By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ62656.
PRIDEiQ62656.

PTM databases

PhosphoSiteiQ62656.

Expressioni

Tissue specificityi

Nervous tissue specific.

Gene expression databases

GenevestigatoriQ62656.

Interactioni

Subunit structurei

The carbonic-anhydrase like domain interacts with CNTN1 (contactin).3 Publications

Protein-protein interaction databases

DIPiDIP-59715N.

Structurei

3D structure databases

ProteinModelPortaliQ62656.
SMRiQ62656. Positions 1699-2285.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 300265Alpha-carbonic anhydraseAdd
BLAST
Domaini314 – 413100Fibronectin type-IIIAdd
BLAST
Domaini1718 – 1993276Tyrosine-protein phosphatase 1Add
BLAST
Domaini2024 – 2283260Tyrosine-protein phosphatase 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1934 – 19407Substrate binding By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi951 – 9544Poly-Ser
Compositional biasi1225 – 12306Poly-Ser
Compositional biasi1426 – 143914Poly-AspAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5599.
HOGENOMiHOG000090262.
HOVERGENiHBG053760.
InParanoidiQ62656.
KOiK08114.
PhylomeDBiQ62656.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.200.10. 1 hit.
3.90.190.10. 2 hits.
InterProiIPR001148. Carbonic_anhydrase_a.
IPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00194. Carb_anhydrase. 1 hit.
PF00041. fn3. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM01057. Carb_anhydrase. 1 hit.
SM00060. FN3. 1 hit.
SM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF51069. SSF51069. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS51144. ALPHA_CA_2. 1 hit.
PS50853. FN3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q62656-1) [UniParc]FASTAAdd to Basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRILQSFLAC VQLLCVCRLD WAYGYYRQQR KLVEEIGWSY TGALNQKNWG     50
KKYPICNSPK QSPINIDEDL TQVNVNLKKL KFQGWEKPSL ENTFIHNTGK 100
TVEINLTNDY YLSGGLSEKV FKASKMTFHW GKCNVSSEGS EHSLEGQKFP 150
LEMQIYCFDA DRFSSFEETV KGKGRLRALS ILFEIGVEEN LDYKAIIDGT 200
ESVSRFGKQA ALDPFILQNL LPNSTDKYYI YNGSLTSPPC TDTVEWIVFK 250
DTVSISESQL AVFCEVLTMQ QSGYVMLMDY LQNNFREQQY KFSRQVFSSY 300
TGKEEIHEAV CSSEPENVQA DPENYTSLLI TWERPRVVYD TMIEKFAVLY 350
QPLEGNDQTK HEFLTDGYQD LGAILNNLIP NMSYVLQIVA ICSNGLYGKY 400
SDQLIVDMPT EDAELDLFPE LIGTEEIIKE ENYGKGNEED TGLNPGRDSA 450
TNQIRKKEPQ VSTTTHYNHM GTKYNEAKTN RSPTRGSEFS GKSDVLNTSL 500
NPTSQQVAEF NPEREMSLPS QIGTNLPPHS VEGTSASLNS GSKTLLVFPQ 550
MNLSGTAESL NMVSITEYKE VSADLSEEEN LLTDFKLDSG ADDSSGSSPA 600
SSTVPFSTDN LSHGYTSSSD TPEAVTYDVL RPESTRNALE DSAPSGSEES 650
LKDPSLEGSV WFPGSTDLTT QSETGSGREG FLQVNSTDFQ VDESRETTET 700
FSPDATASRG PSVTDMEMPH YSTFAYPPTE VTSHAFTPSS RPLDLAPTSN 750
ILHSQTTQPV YNGETPLQPS YSSEVFPLVT PLLLDNQTLN TTPAASSSDS 800
ALHATPVFPS VGVSFDSILS SYDDAPLLPF SSASFSSDLF HHLHTVSQTL 850
PQVTSAAERD ELSLHASLLV AGGDLLLEPS LVQYSDVMSH QVTIHAASDT 900
LEFGSESAVL YKTSMVSQIE SPSSDVVMHA YSSGPETSYA IEGSHHVLTV 950
SSSSAIPVHD SVGVADQGSL LINPSHISLP ESSFITPTAS LLQLPPALSG 1000
DGEWSGASSD SELLLPDTDG LRTLNMSSPV SVADFTYTTS VSGDDIKPLS 1050
KGEMMYGNET ELKMSSFSDM AYPSKSTVVP KMSDIVNKWS ESLKETSVSV 1100
SSINSVFTES LVYPITKVFD QEISRVPEII FPVKPTHTAS QASGDTWLKP 1150
GLSTNSEPAL SDTASSEVSH PSTQPLLYEA ASPFNTEALL QPSFPASDVD 1200
TLLKTALPSG PRDPVLTETP MVEQSSSSVS LPLASESASS KSTLHFTSVP 1250
VLNMSPSDVH PTSLQRLTVP HSREEYFEQG LLKSKSPQQV LPSLHSHDEF 1300
FQTAHLDISQ AYPPKGRHAF ATPILSINEP QNTLINRLVY SEDIFMHPEI 1350
SITDKALTGL PTTVSDVLIA TDHSVPLGSG PISMTTVSPN RDDSVTTTKL 1400
LLPSKATSKP THSARSDADL VGGGEDGDDY DDDDYDDIDS DRFPVNKCMS 1450
CSPYRESQEK VMNDSDTQES SLVDQSDPIS HLLSENTEEE NGGTGVTRVD 1500
KSPDKSPPPS MLPQKHNDGR EDRDIQMGSA VLPHTPGSKA WAVLTSDEES 1550
GSGQGTSDSL NDNETSTDFS FPDVNEKDAD GVLEADDTGI APGSPRSSTP 1600
SVTSGHSGVS NSSEAEASNS SHESRIGLAE GLESEKKAVI PLVIVSALTF 1650
ICLVVLVGIL IYWRKCFQTA HFYLEDNTSP RVISTPPTPI FPISDDIGAI 1700
PIKHFPKHVA DLHASNGFTE EFETLKEFYQ EVQSCTVDLG ITADSSNHPD 1750
NKHKNRYVNI VAYDHSRVKL TQLAEKDGKL TDYINANYVD GYNRPKAYIA 1800
AQGPLKSTAE DFWRMIWEHN VEVIVMITNL VEKGRRKCDQ YWPTDGSEEY 1850
GSFLVNQKNV QVLAYYTVRN FTLRNTKIKK GSQKGRSSGR LVTQYHYTQW 1900
PDMGVPEYSL PVLAFVRKTA QAKRHAVGPV VVHCSAGVGR TGTYIVLDSM 1950
LQQIQHEGTV NIFGFLKHIR SQRNYLVQTE EQYVFIHDTL VEAILSKETE 2000
VPDSHIHSYV NTLLIPGPSG KTKLEKQFQL LSQSNILQSD YSTALKQCNR 2050
EKNRTSSIIP VERSRVGISS LSGEGTDYIN ASYIMGYYQS NEFIITQHPL 2100
LHTIKDFWRM IWDHNAQLVV MIPDGQNMAE DEFVYWPNKD EPINCESFKV 2150
TLMSEEHKCL SNEEKLIVQD FILEATQDDY VLEVRHFQCP KWPNPDSPIS 2200
KTFELISIIK EEAANRDGPM IVHDEHGGVT AGTFCALTTL MHQLEKENSM 2250
DVYQVAKMIN LMRPGVFTDI EQYQFLYKVV LSLVSTRQEE NPSTSLDSNG 2300
AALPDGNIAE SLESLV 2316
Length:2,316
Mass (Da):255,342
Last modified:November 1, 1996 - v1
Checksum:i419EA9B89BDD165F
GO
Isoform 2 (identifier: Q62656-2) [UniParc]FASTAAdd to Basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     763-1615: Missing.

Show »
Length:1,463
Mass (Da):164,596
Checksum:iFF524D7DA90C4BE0
GO
Isoform 3 (identifier: Q62656-3) [UniParc]FASTAAdd to Basket

Also known as: Phosphacan

The sequence of this isoform differs from the canonical sequence as follows:
     1616-1616: E → G
     1617-2316: Missing.

Show »
Length:1,616
Mass (Da):175,839
Checksum:i6F02DDE391A41117
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei763 – 1615853Missing in isoform 2. VSP_005152Add
BLAST
Alternative sequencei1616 – 16161E → G in isoform 3. VSP_005153
Alternative sequencei1617 – 2316700Missing in isoform 3. VSP_005154Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09357 mRNA. Translation: AAC52207.1.
U04998 mRNA. Translation: AAC52383.1.
PIRiA40169.
RefSeqiNP_001164156.1. NM_001170685.1.
NP_037212.2. NM_013080.2.
UniGeneiRn.10088.

Genome annotation databases

GeneIDi25613.
KEGGirno:25613.
UCSCiRGD:3455. rat. [Q62656-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09357 mRNA. Translation: AAC52207.1 .
U04998 mRNA. Translation: AAC52383.1 .
PIRi A40169.
RefSeqi NP_001164156.1. NM_001170685.1.
NP_037212.2. NM_013080.2.
UniGenei Rn.10088.

3D structure databases

ProteinModelPortali Q62656.
SMRi Q62656. Positions 1699-2285.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59715N.

PTM databases

PhosphoSitei Q62656.

Proteomic databases

PaxDbi Q62656.
PRIDEi Q62656.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 25613.
KEGGi rno:25613.
UCSCi RGD:3455. rat. [Q62656-1 ]

Organism-specific databases

CTDi 5803.
RGDi 3455. Ptprz1.

Phylogenomic databases

eggNOGi COG5599.
HOGENOMi HOG000090262.
HOVERGENi HBG053760.
InParanoidi Q62656.
KOi K08114.
PhylomeDBi Q62656.

Miscellaneous databases

NextBioi 607359.
PROi Q62656.

Gene expression databases

Genevestigatori Q62656.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
3.10.200.10. 1 hit.
3.90.190.10. 2 hits.
InterProi IPR001148. Carbonic_anhydrase_a.
IPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view ]
Pfami PF00194. Carb_anhydrase. 1 hit.
PF00041. fn3. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00700. PRTYPHPHTASE.
SMARTi SM01057. Carb_anhydrase. 1 hit.
SM00060. FN3. 1 hit.
SM00194. PTPc. 2 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 1 hit.
SSF51069. SSF51069. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEi PS51144. ALPHA_CA_2. 1 hit.
PS50853. FN3. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and molecular variants of rat receptor-type protein tyrosine phosphatase-zeta/beta."
    Maurel P., Meyer-Puttlitz B., Flad M., Margolis R.U., Margolis R.K.
    DNA Seq. 5:323-328(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Phosphacan, a chondroitin sulfate proteoglycan of brain that interacts with neurons and neural cell-adhesion molecules, is an extracellular variant of a receptor-type protein tyrosine phosphatase."
    Maurel P., Rauch U., Flad M., Margolis R.K., Margolis R.U.
    Proc. Natl. Acad. Sci. U.S.A. 91:2512-2516(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "Interactions of the chondroitin sulfate proteoglycan phosphacan, the extracellular domain of a receptor-type protein tyrosine phosphatase, with neurons, glia, and neural cell adhesion molecules."
    Milev P., Friedlander D.R., Sakurai T., Karthikeyan L., Flad M., Margolis R.K., Grumet M., Margolis R.U.
    J. Cell Biol. 127:1703-1715(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH N-CAM AND NG-CAM.
  4. "Interactions with tenascin and differential effects on cell adhesion of neurocan and phosphacan, two major chondroitin sulfate proteoglycans of nervous tissue."
    Grumet M., Milev P., Sakurai T., Karthikeyan L., Bourdon M., Margolis R.K., Margolis R.U.
    J. Biol. Chem. 269:12142-12146(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TENASCIN.
  5. "The carbonic anhydrase domain of receptor tyrosine phosphatase beta is a functional ligand for the axonal cell recognition molecule contactin."
    Peles E., Nativ M., Campbell P.L., Sakurai T., Martinez R., Lev S., Clary D.O., Schilling J., Barnea G., Plowman G.D., Grumet M., Schlessinger J.
    Cell 82:251-260(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CONTACTIN.

Entry informationi

Entry nameiPTPRZ_RAT
AccessioniPrimary (citable) accession number: Q62656
Secondary accession number(s): Q62621
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi