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Protein

Transcription factor 4

Gene

Tcf4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Interacts with the CCAAT displacement protein (CDP2) to bind the tyrosine hydroxylase enhancer.By similarity

GO - Molecular functioni

  • bHLH transcription factor binding Source: UniProtKB
  • double-stranded DNA binding Source: RGD
  • E-box binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 4
Short name:
TCF-4
Alternative name(s):
Immunoglobulin transcription factor 2
Short name:
ITF-2
Short name:
RITF-2
R8f DNA-binding protein
SL3-3 enhancer factor 2
Short name:
SEF-2
Gene namesi
Name:Tcf4
Synonyms:Itf2, Sef2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69271. Tcf4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 589589Transcription factor 4PRO_0000127258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei290 – 2901PhosphoserineCombined sources
Modified residuei433 – 4331PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ62655.
PRIDEiQ62655.

PTM databases

iPTMnetiQ62655.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin. Interacts with HIVEP2. Interacts with NEUROD2 (By similarity). Interacts with AGBL1 (By similarity).By similarity

GO - Molecular functioni

  • bHLH transcription factor binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020431.

Structurei

3D structure databases

ProteinModelPortaliQ62655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini486 – 53954bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 15722Leucine-zipperAdd
BLAST
Regioni380 – 40324Class A specific domainAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3910. Eukaryota.
ENOG410XYUA. LUCA.
HOGENOMiHOG000234180.
HOVERGENiHBG003854.
InParanoidiQ62655.
KOiK15603.
PhylomeDBiQ62655.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62655-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSRDLGSHD NLSPPFANSR IQSKTERGSY SSYGRENVQG CHQSLLGGDM
60 70 80 90 100
DMGNPGTLSP TKPGSQYYPY SSNNARRRPL HSSTMEVQTK KVRKVPPGLP
110 120 130 140 150
SSVYAPSAST ADYNRDSPGY SSSKPAASTF SSSFFMQDGH HSSDPWSSSS
160 170 180 190 200
GMNQPGYGGM LGNSHIPQSS SYCSLHPHER LSYPSHSSAD INSSLPPMST
210 220 230 240 250
FHRSGTNHYS TSSCTPPANG TDSIMANRGT GAAGSSQTGD ALGKALASIY
260 270 280 290 300
SPDHTNNSFS SNPSTPVGSP PSLSAGTAVW SRNGGQASSS PNYEGPLHSL
310 320 330 340 350
QSRIEDRLER LDDAIHVLRN HAVGPSTAVP GGHGDMHGII GPSHNGAMGS
360 370 380 390 400
LGSGYGTGLL SANRHSLMVG AHREDGVALR GSHSLLPNQV PVPQLPVQSA
410 420 430 440 450
TSPDLNPPQD PYRGMPPGLQ GQSVSSGSSE IKSDDEGDEN LQDTKSSEDK
460 470 480 490 500
KLDDDKKDIK SITRSRSSNN DDEDLTPEQK AEREKERRMA NNARERLRVR
510 520 530 540 550
DINEAFKELG RMVQLHLKSD KPQTKLLILH QAVAVILSLE QQVRERNLNP
560 570 580
KAACLKRREE EKVSSEPPPL SLAGPHPGMG DTANHMGQM
Note: No experimental confirmation available.
Length:589
Mass (Da):63,053
Last modified:December 6, 2005 - v2
Checksum:iD2A271D197D1058C
GO
Isoform 2 (identifier: Q62655-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     464-467: Missing.

Show »
Length:585
Mass (Da):62,567
Checksum:i2DEF03D3508F3CE7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti582 – 5821T → A in AAA21122 (PubMed:7913462).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei464 – 4674Missing in isoform 2. 2 PublicationsVSP_002116

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149284 mRNA. Translation: AAK26670.1.
U09228 mRNA. Translation: AAA21122.1.
PIRiA53689.
RefSeqiNP_445821.1. NM_053369.1. [Q62655-2]
UniGeneiRn.23354.

Genome annotation databases

GeneIDi84382.
KEGGirno:84382.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149284 mRNA. Translation: AAK26670.1.
U09228 mRNA. Translation: AAA21122.1.
PIRiA53689.
RefSeqiNP_445821.1. NM_053369.1. [Q62655-2]
UniGeneiRn.23354.

3D structure databases

ProteinModelPortaliQ62655.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020431.

PTM databases

iPTMnetiQ62655.

Proteomic databases

PaxDbiQ62655.
PRIDEiQ62655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84382.
KEGGirno:84382.

Organism-specific databases

CTDi6925.
RGDi69271. Tcf4.

Phylogenomic databases

eggNOGiKOG3910. Eukaryota.
ENOG410XYUA. LUCA.
HOGENOMiHOG000234180.
HOVERGENiHBG003854.
InParanoidiQ62655.
KOiK15603.
PhylomeDBiQ62655.

Miscellaneous databases

PROiQ62655.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and analysis of a novel helix-loop-helix transcription factor-encoding gene from Rat forebrain."
    Diaz M.I., Lopez J., Padilla S., Madrazo J.A., Capo D., Cabrero N., Felix C., Ossorio J., Olmeda Y., Lugo D.I.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Forebrain.
  2. "Isolation of two E-box binding factors that interact with the rat tyrosine hydroxylase enhancer."
    Yoon S.O., Chikaraishi D.M.
    J. Biol. Chem. 269:18453-18462(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 162-589 (ISOFORMS 1 AND 2).
    Strain: New England Deaconess Hospital.
    Tissue: Adrenal medulla.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-290, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiITF2_RAT
AccessioniPrimary (citable) accession number: Q62655
Secondary accession number(s): Q99N33
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 6, 2005
Last modified: June 8, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.