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Protein

Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial

Gene

Ech1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei173Substrate; via amide nitrogenBy similarity1
Sitei196Important for catalytic activity1 Publication1
Sitei204Important for catalytic activity1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00659.

Chemistry databases

SwissLipidsiSLP:000001100.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial (EC:5.3.3.-)
Gene namesi
Name:Ech1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi69353. Ech1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • membrane Source: Ensembl
  • mitochondrion Source: UniProtKB
  • peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33MitochondrionSequence analysisAdd BLAST33
ChainiPRO_000000741934 – 327Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrialAdd BLAST294

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei230N6-succinyllysineBy similarity1
Modified residuei267PhosphoserineBy similarity1
Modified residuei316N6-succinyllysineBy similarity1
Modified residuei326N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ62651.
PRIDEiQ62651.

PTM databases

iPTMnetiQ62651.
PhosphoSitePlusiQ62651.

Expressioni

Gene expression databases

BgeeiENSRNOG00000020308.
GenevisibleiQ62651. RN.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

IntActiQ62651. 1 interactor.
MINTiMINT-4597589.
STRINGi10116.ENSRNOP00000027537.

Structurei

Secondary structure

1327
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi55 – 63Combined sources9
Beta strandi66 – 71Combined sources6
Helixi74 – 76Combined sources3
Helixi82 – 96Combined sources15
Beta strandi103 – 109Combined sources7
Helixi119 – 126Combined sources8
Helixi134 – 157Combined sources24
Beta strandi158 – 160Combined sources3
Beta strandi162 – 166Combined sources5
Beta strandi168 – 171Combined sources4
Helixi173 – 178Combined sources6
Beta strandi181 – 187Combined sources7
Beta strandi191 – 193Combined sources3
Helixi196 – 199Combined sources4
Helixi207 – 210Combined sources4
Helixi211 – 213Combined sources3
Helixi218 – 227Combined sources10
Beta strandi230 – 232Combined sources3
Helixi233 – 238Combined sources6
Beta strandi241 – 248Combined sources8
Helixi249 – 265Combined sources17
Helixi268 – 283Combined sources16
Helixi286 – 300Combined sources15
Helixi304 – 314Combined sources11
Helixi319 – 321Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCIX-ray1.50A/B/C54-327[»]
ProteinModelPortaliQ62651.
SMRiQ62651.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62651.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni115 – 119Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi325 – 327Microbody targeting signalSequence analysis3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1681. Eukaryota.
ENOG410XTHX. LUCA.
GeneTreeiENSGT00760000119100.
HOGENOMiHOG000027939.
HOVERGENiHBG005556.
InParanoidiQ62651.
KOiK12663.
OMAiSYMRDHR.
OrthoDBiEOG091G0H26.
PhylomeDBiQ62651.
TreeFamiTF314317.

Family and domain databases

Gene3Di1.10.12.10. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR014748. Crontonase_C.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62651-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAMTVSSK LLGLLMQQLR GTRQLYFNVS LRSLSSSAQE ASKRIPEEVS
60 70 80 90 100
DHNYESIQVT SAQKHVLHVQ LNRPEKRNAM NRAFWRELVE CFQKISKDSD
110 120 130 140 150
CRAVVVSGAG KMFTSGIDLM DMASDILQPP GDDVARIAWY LRDLISRYQK
160 170 180 190 200
TFTVIEKCPK PVIAAIHGGC IGGGVDLISA CDIRYCTQDA FFQVKEVDVG
210 220 230 240 250
LAADVGTLQR LPKVIGNRSL VNELTFTARK MMADEALDSG LVSRVFPDKD
260 270 280 290 300
VMLNAAFALA ADISSKSPVA VQGSKINLIY SRDHSVDESL DYMATWNMSM
310 320
LQTQDIIKSV QAAMEKKDSK SITFSKL
Length:327
Mass (Da):36,172
Last modified:July 15, 1999 - v2
Checksum:i6FAE35D7D5F66BC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164A → T in AAA82008 (PubMed:7558027).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08976 mRNA. Translation: AAA82008.1.
BC062226 mRNA. Translation: AAH62226.1.
PIRiA57626.
RefSeqiNP_072116.1. NM_022594.1.
UniGeneiRn.6148.

Genome annotation databases

EnsembliENSRNOT00000027537; ENSRNOP00000027537; ENSRNOG00000020308.
GeneIDi64526.
KEGGirno:64526.
UCSCiRGD:69353. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08976 mRNA. Translation: AAA82008.1.
BC062226 mRNA. Translation: AAH62226.1.
PIRiA57626.
RefSeqiNP_072116.1. NM_022594.1.
UniGeneiRn.6148.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DCIX-ray1.50A/B/C54-327[»]
ProteinModelPortaliQ62651.
SMRiQ62651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ62651. 1 interactor.
MINTiMINT-4597589.
STRINGi10116.ENSRNOP00000027537.

Chemistry databases

SwissLipidsiSLP:000001100.

PTM databases

iPTMnetiQ62651.
PhosphoSitePlusiQ62651.

Proteomic databases

PaxDbiQ62651.
PRIDEiQ62651.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027537; ENSRNOP00000027537; ENSRNOG00000020308.
GeneIDi64526.
KEGGirno:64526.
UCSCiRGD:69353. rat.

Organism-specific databases

CTDi1891.
RGDi69353. Ech1.

Phylogenomic databases

eggNOGiKOG1681. Eukaryota.
ENOG410XTHX. LUCA.
GeneTreeiENSGT00760000119100.
HOGENOMiHOG000027939.
HOVERGENiHBG005556.
InParanoidiQ62651.
KOiK12663.
OMAiSYMRDHR.
OrthoDBiEOG091G0H26.
PhylomeDBiQ62651.
TreeFamiTF314317.

Enzyme and pathway databases

UniPathwayiUPA00659.

Miscellaneous databases

EvolutionaryTraceiQ62651.
PROiQ62651.

Gene expression databases

BgeeiENSRNOG00000020308.
GenevisibleiQ62651. RN.

Family and domain databases

Gene3Di1.10.12.10. 1 hit.
3.90.226.10. 1 hit.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR014748. Crontonase_C.
IPR001753. Crotonase_core_superfam.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]
PfamiPF00378. ECH_1. 1 hit.
[Graphical view]
SUPFAMiSSF52096. SSF52096. 1 hit.
PROSITEiPS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECH1_RAT
AccessioniPrimary (citable) accession number: Q62651
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1999
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.