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Q62639

- RHEB_RAT

UniProt

Q62639 - RHEB_RAT

Protein

GTP-binding protein Rheb

Gene

Rheb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity By similarity.By similarity

    Enzyme regulationi

    Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2 By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi20 – 201MagnesiumBy similarity
    Metal bindingi38 – 381MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 216GTPBy similarity
    Nucleotide bindingi32 – 387GTPBy similarity
    Nucleotide bindingi149 – 1502GTPBy similarity

    GO - Molecular functioni

    1. GDP binding Source: RGD
    2. GTPase activity Source: RGD
    3. GTP binding Source: RGD
    4. metal ion binding Source: UniProtKB-KW
    5. protein binding Source: RGD

    GO - Biological processi

    1. positive regulation of TOR signaling Source: UniProtKB
    2. regulation of neuronal synaptic plasticity Source: RGD
    3. small GTPase mediated signal transduction Source: RGD

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein Rheb
    Alternative name(s):
    Ras homolog enriched in brain
    Gene namesi
    Name:Rheb
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621840. Rheb.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. dendrite Source: RGD
    2. neuronal cell body Source: RGD
    3. plasma membrane Source: UniProtKB-SubCell
    4. spliceosomal complex Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 181181GTP-binding protein RhebPRO_0000082710Add
    BLAST
    Propeptidei182 – 1843Removed in mature formBy similarityPRO_0000281367

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei130 – 1301Phosphoserine; by MAPKAPK5By similarity
    Modified residuei181 – 1811Cysteine methyl esterBy similarity
    Lipidationi181 – 1811S-farnesyl cysteineBy similarity

    Post-translational modificationi

    Farnesylation is important for efficiently activating mTORC1-mediated signaling.By similarity
    Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.By similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    PRIDEiQ62639.

    PTM databases

    PhosphoSiteiQ62639.

    Expressioni

    Tissue specificityi

    Expressed at high levels in normal adult cortex as well as a number of peripheral tissues, including lung and intestine.

    Gene expression databases

    GenevestigatoriQ62639.

    Interactioni

    Subunit structurei

    Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2 By similarity.By similarity

    Structurei

    Secondary structure

    1
    184
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 137
    Helixi19 – 279
    Beta strandi41 – 499
    Beta strandi52 – 609
    Beta strandi63 – 675
    Beta strandi69 – 735
    Beta strandi79 – 8810
    Helixi90 – 10718
    Beta strandi114 – 1196
    Helixi121 – 1233
    Turni124 – 1263
    Helixi131 – 14111
    Beta strandi144 – 1474
    Helixi153 – 17321

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2L0XNMR-A6-174[»]
    ProteinModelPortaliQ62639.
    SMRiQ62639. Positions 2-171.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ62639.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi35 – 439Effector region

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rheb family.Curated

    Phylogenomic databases

    HOVERGENiHBG009351.
    KOiK07208.
    PhylomeDBiQ62639.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view]
    PANTHERiPTHR24070. PTHR24070. 1 hit.
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00173. RAS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51421. RAS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q62639-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN    50
    GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH 100
    GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA 150
    KENQTAVDVF RRIILEAEKI DGAASQGKSS CSVM 184
    Length:184
    Mass (Da):20,479
    Last modified:November 1, 1996 - v1
    Checksum:i8F4C8080BDF92DE9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08227 mRNA. Translation: AAA21380.1.
    BC088857 mRNA. Translation: AAH88857.1.
    PIRiI55401.
    RefSeqiNP_037348.1. NM_013216.1.
    UniGeneiRn.859.

    Genome annotation databases

    GeneIDi26954.
    KEGGirno:26954.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08227 mRNA. Translation: AAA21380.1 .
    BC088857 mRNA. Translation: AAH88857.1 .
    PIRi I55401.
    RefSeqi NP_037348.1. NM_013216.1.
    UniGenei Rn.859.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2L0X NMR - A 6-174 [» ]
    ProteinModelPortali Q62639.
    SMRi Q62639. Positions 2-171.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q62639.

    Proteomic databases

    PRIDEi Q62639.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 26954.
    KEGGi rno:26954.

    Organism-specific databases

    CTDi 6009.
    RGDi 621840. Rheb.

    Phylogenomic databases

    HOVERGENi HBG009351.
    KOi K07208.
    PhylomeDBi Q62639.

    Miscellaneous databases

    EvolutionaryTracei Q62639.
    NextBioi 608022.
    PROi Q62639.

    Gene expression databases

    Genevestigatori Q62639.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR020849. Small_GTPase_Ras.
    [Graphical view ]
    PANTHERi PTHR24070. PTHR24070. 1 hit.
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00173. RAS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51421. RAS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein."
      Yamagata K., Sanders L.K., Kaufmann W.E., Yee W., Barnes C.A., Nathans D., Worley P.F.
      J. Biol. Chem. 269:16333-16339(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiRHEB_RAT
    AccessioniPrimary (citable) accession number: Q62639
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The conserved catalytic Gln-64 found in other Ras-like GTPases seems not to be involved in GTP hydrolysis in RHEB.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3