GTP-binding protein Rheb precursor - Rattus norvegicus (Rat)

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Q62639 (RHEB_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTP-binding protein Rheb

Alternative name(s):
Ras homolog enriched in brain

Gene names

Name:

Rheb

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity By similarity.
Enzyme regulation	Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2 By similarity.
Subunit structure	Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2 By similarity.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Tissue specificity	Expressed at high levels in normal adult cortex as well as a number of peripheral tissues, including lung and intestine.
Post-translational modification	Farnesylation is important for efficiently activating mTORC1-mediated signaling By similarity. Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation By similarity.
Miscellaneous	The conserved catalytic Gln-64 found in other Ras-like GTPases seems not to be involved in GTP hydrolysis in RHEB By similarity.
Sequence similarities	Belongs to the small GTPase superfamily. Rheb family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of TOR signaling cascade Inferred from sequence or structural similarity. Source: UniProtKB regulation of neuronal synaptic plasticity Non-traceable author statement Ref.1. Source: RGD small GTPase mediated signal transduction Inferred from direct assay PubMed 16354680. Source: RGD
Cellular_component	dendrite Inferred from direct assay PubMed 15525761. Source: RGD neuronal cell body Inferred from direct assay PubMed 15525761. Source: RGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosomal complex Inferred from electronic annotation. Source: Compara
Molecular_function	GDP binding Inferred from direct assay PubMed 16354680. Source: RGD GTP binding Inferred from direct assay PubMed 16354680. Source: RGD GTPase activity Inferred from direct assay PubMed 16354680. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 181

181

GTP-binding protein Rheb

PRO_0000082710

Propeptide

182 – 184

Removed in mature form By similarity

PRO_0000281367

Regions

Nucleotide binding

16 – 21

GTP By similarity

Nucleotide binding

32 – 38

GTP By similarity

Nucleotide binding

149 – 150

GTP By similarity

Motif

35 – 43

Effector region By similarity

Sites

Metal binding

Magnesium By similarity

Metal binding

Magnesium By similarity

Amino acid modifications

Modified residue

130

Phosphoserine; by MAPKAPK5 By similarity

Modified residue

181

Cysteine methyl ester By similarity

Lipidation

181

S-farnesyl cysteine By similarity

Secondary structure

184

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q62639 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8F4C8080BDF92DE9
Show »

FASTA

184

20,479

        10         20         30         40         50         60 
MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN GQEYHLQLVD 

        70         80         90        100        110        120 
TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH GKLLDMVGKV QIPIMLVGNK 

       130        140        150        160        170        180 
KDLHMERVIS YEEGKALAES WNAAFLESSA KENQTAVDVF RRIILEAEKI DGAASQGKSS 


CSVM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein." Yamagata K., Sanders L.K., Kaufmann W.E., Yee W., Barnes C.A., Nathans D., Worley P.F. J. Biol. Chem. 269:16333-16339(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Hippocampus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U08227 mRNA. Translation: AAA21380.1.
BC088857 mRNA. Translation: AAH88857.1.

IPI

IPI00207070.

PIR

I55401.

RefSeq

NP_037348.1. NM_013216.1.

UniGene

Rn.859.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L0X	NMR	-	A	6-174	[»]

ProteinModelPortal

Q62639.

SMR

Q62639. Positions 2-171.

ModBase

Search...

PTM databases

PhosphoSite

Q62639.

Proteomic databases

PRIDE

Q62639.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

26954.

KEGG

rno:26954.

Organism-specific databases

CTD

6009.

RGD

621840. Rheb.

Phylogenomic databases

HOVERGEN

HBG009351.

K07208.

OrthoDB

EOG4VMFGG.

Gene expression databases

Genevestigator

Q62639.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]

PANTHER

PTHR24070. PTHR24070. 1 hit.

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00173. RAS. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51421. RAS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q62639.

NextBio

608022.

Entry information

Entry name

RHEB_RAT

Accession

Primary (citable) accession number: Q62639

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents