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Q62639

- RHEB_RAT

UniProt

Q62639 - RHEB_RAT

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Protein

GTP-binding protein Rheb

Gene

Rheb

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity (By similarity).By similarity

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi20 – 201MagnesiumBy similarity
Metal bindingi38 – 381MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 216GTPBy similarity
Nucleotide bindingi32 – 387GTPBy similarity
Nucleotide bindingi149 – 1502GTPBy similarity

GO - Molecular functioni

  1. GDP binding Source: RGD
  2. GTPase activity Source: RGD
  3. GTP binding Source: RGD
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. positive regulation of TOR signaling Source: UniProtKB
  2. regulation of neuronal synaptic plasticity Source: RGD
  3. small GTPase mediated signal transduction Source: RGD
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein Rheb
Alternative name(s):
Ras homolog enriched in brain
Gene namesi
Name:Rheb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621840. Rheb.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

GO - Cellular componenti

  1. dendrite Source: RGD
  2. extracellular vesicular exosome Source: Ensembl
  3. neuronal cell body Source: RGD
  4. plasma membrane Source: UniProtKB-KW
  5. spliceosomal complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181GTP-binding protein RhebPRO_0000082710Add
BLAST
Propeptidei182 – 1843Removed in mature formBy similarityPRO_0000281367

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301Phosphoserine; by MAPKAPK5By similarity
Modified residuei181 – 1811Cysteine methyl esterBy similarity
Lipidationi181 – 1811S-farnesyl cysteineBy similarity

Post-translational modificationi

Farnesylation is important for efficiently activating mTORC1-mediated signaling.By similarity
Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PRIDEiQ62639.

PTM databases

PhosphoSiteiQ62639.

Expressioni

Tissue specificityi

Expressed at high levels in normal adult cortex as well as a number of peripheral tissues, including lung and intestine.

Gene expression databases

ExpressionAtlasiQ62639. baseline.
GenevestigatoriQ62639.

Interactioni

Subunit structurei

Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2 (By similarity).By similarity

Structurei

Secondary structure

1
184
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 137
Helixi19 – 279
Beta strandi41 – 499
Beta strandi52 – 609
Beta strandi63 – 675
Beta strandi69 – 735
Beta strandi79 – 8810
Helixi90 – 10718
Beta strandi114 – 1196
Helixi121 – 1233
Turni124 – 1263
Helixi131 – 14111
Beta strandi144 – 1474
Helixi153 – 17321

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L0XNMR-A6-174[»]
ProteinModelPortaliQ62639.
SMRiQ62639. Positions 2-171.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62639.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi35 – 439Effector region

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rheb family.Curated

Phylogenomic databases

HOVERGENiHBG009351.
InParanoidiQ62639.
KOiK07208.
PhylomeDBiQ62639.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00173. RAS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62639-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPQSKSRKIA ILGYRSVGKS SLTIQFVEGQ FVDSYDPTIE NTFTKLITVN
60 70 80 90 100
GQEYHLQLVD TAGQDEYSIF PQTYSIDING YILVYSVTSI KSFEVIKVIH
110 120 130 140 150
GKLLDMVGKV QIPIMLVGNK KDLHMERVIS YEEGKALAES WNAAFLESSA
160 170 180
KENQTAVDVF RRIILEAEKI DGAASQGKSS CSVM
Length:184
Mass (Da):20,479
Last modified:November 1, 1996 - v1
Checksum:i8F4C8080BDF92DE9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U08227 mRNA. Translation: AAA21380.1.
BC088857 mRNA. Translation: AAH88857.1.
PIRiI55401.
RefSeqiNP_037348.1. NM_013216.1.
UniGeneiRn.859.

Genome annotation databases

GeneIDi26954.
KEGGirno:26954.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U08227 mRNA. Translation: AAA21380.1 .
BC088857 mRNA. Translation: AAH88857.1 .
PIRi I55401.
RefSeqi NP_037348.1. NM_013216.1.
UniGenei Rn.859.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2L0X NMR - A 6-174 [» ]
ProteinModelPortali Q62639.
SMRi Q62639. Positions 2-171.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q62639.

Proteomic databases

PRIDEi Q62639.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 26954.
KEGGi rno:26954.

Organism-specific databases

CTDi 6009.
RGDi 621840. Rheb.

Phylogenomic databases

HOVERGENi HBG009351.
InParanoidi Q62639.
KOi K07208.
PhylomeDBi Q62639.

Miscellaneous databases

EvolutionaryTracei Q62639.
NextBioi 608022.
PROi Q62639.

Gene expression databases

ExpressionAtlasi Q62639. baseline.
Genevestigatori Q62639.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view ]
PANTHERi PTHR24070. PTHR24070. 1 hit.
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00173. RAS. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51421. RAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rheb, a growth factor- and synaptic activity-regulated gene, encodes a novel Ras-related protein."
    Yamagata K., Sanders L.K., Kaufmann W.E., Yee W., Barnes C.A., Nathans D., Worley P.F.
    J. Biol. Chem. 269:16333-16339(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiRHEB_RAT
AccessioniPrimary (citable) accession number: Q62639
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The conserved catalytic Gln-64 found in other Ras-like GTPases seems not to be involved in GTP hydrolysis in RHEB.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3