Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras-related protein Rap-1b

Gene

Rap1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function (By similarity).By similarity

Enzyme regulationi

Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18GTPBy similarity9
Nucleotide bindingi57 – 61GTPBy similarity5
Nucleotide bindingi116 – 119GTPBy similarity4
Nucleotide bindingi147 – 149GTPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-354192. Integrin alphaIIb beta3 signaling.
R-RNO-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-RNO-372708. p130Cas linkage to MAPK signaling for integrins.
R-RNO-392517. Rap1 signalling.
R-RNO-6798695. Neutrophil degranulation.
R-RNO-8875555. MET activates RAP1 and RAC1.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-1b
Alternative name(s):
GTP-binding protein smg p21B
Gene namesi
Name:Rap1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620577. Rap1b.

Subcellular locationi

  • Cell membrane By similarity
  • Cytoplasmcytosol By similarity
  • Cell junction By similarity

  • Note: May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000302131 – 181Ras-related protein Rap-1bAdd BLAST181
PropeptideiPRO_0000030214182 – 184Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei39ADP-ribosylserine; by botulinum toxinBy similarity1
Modified residuei179Phosphoserine; by PKABy similarity1
Modified residuei181Cysteine methyl esterBy similarity1
Lipidationi181S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

ADP-ribosylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ62636.
PRIDEiQ62636.

PTM databases

iPTMnetiQ62636.
PhosphoSitePlusiQ62636.

Expressioni

Gene expression databases

BgeeiENSRNOG00000007048.
GenevisibleiQ62636. RN.

Interactioni

Subunit structurei

Interacts with SGSM1, SGSM2 and SGSM3. Interacts with KRIT1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ62636. 1 interactor.
MINTiMINT-4542540.
STRINGi10116.ENSRNOP00000009511.

Structurei

Secondary structure

1184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Helixi16 – 25Combined sources10
Beta strandi39 – 46Combined sources8
Beta strandi49 – 57Combined sources9
Helixi61 – 63Combined sources3
Helixi68 – 74Combined sources7
Beta strandi76 – 83Combined sources8
Helixi87 – 104Combined sources18
Beta strandi111 – 116Combined sources6
Helixi121 – 123Combined sources3
Helixi128 – 137Combined sources10
Beta strandi143 – 145Combined sources3
Turni148 – 151Combined sources4
Helixi154 – 165Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3X1WX-ray1.20A1-167[»]
3X1XX-ray1.00A1-167[»]
3X1YX-ray1.17A1-167[»]
3X1ZX-ray1.25A/B1-167[»]
ProteinModelPortaliQ62636.
SMRiQ62636.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 67Interaction with KRIT1By similarityAdd BLAST43

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi32 – 40Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133678.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ62636.
KOiK07836.
OMAiRDSTHPR.
OrthoDBiEOG091G0OOG.
PhylomeDBiQ62636.
TreeFamiTF313014.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWSN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VPGKARKKSS CQLL
Length:184
Mass (Da):20,798
Last modified:August 16, 2004 - v2
Checksum:i37A76895E58DD80C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58T → A in AAA92787 (Ref. 1) Curated1
Sequence conflicti75G → E in AAA92787 (Ref. 1) Curated1
Sequence conflicti86 – 90AQSTF → TQIDC in AAA92787 (Ref. 1) Curated5
Sequence conflicti95D → G in AAA92787 (Ref. 1) Curated1
Sequence conflicti127G → P in AAA92787 (Ref. 1) Curated1
Sequence conflicti174 – 175KA → NR in AAA92787 (Ref. 1) Curated2
Sequence conflicti180S → P in AAA92787 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07795 mRNA. Translation: AAA92787.1.
AY607847 mRNA. Translation: AAT37620.1.
BC081731 mRNA. Translation: AAH81731.1.
RefSeqiNP_599173.2. NM_134346.3.
XP_008763599.1. XM_008765377.2.
UniGeneiRn.95071.

Genome annotation databases

EnsembliENSRNOT00000009511; ENSRNOP00000009511; ENSRNOG00000007048.
GeneIDi171337.
KEGGirno:171337.
UCSCiRGD:620577. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07795 mRNA. Translation: AAA92787.1.
AY607847 mRNA. Translation: AAT37620.1.
BC081731 mRNA. Translation: AAH81731.1.
RefSeqiNP_599173.2. NM_134346.3.
XP_008763599.1. XM_008765377.2.
UniGeneiRn.95071.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3X1WX-ray1.20A1-167[»]
3X1XX-ray1.00A1-167[»]
3X1YX-ray1.17A1-167[»]
3X1ZX-ray1.25A/B1-167[»]
ProteinModelPortaliQ62636.
SMRiQ62636.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ62636. 1 interactor.
MINTiMINT-4542540.
STRINGi10116.ENSRNOP00000009511.

PTM databases

iPTMnetiQ62636.
PhosphoSitePlusiQ62636.

Proteomic databases

PaxDbiQ62636.
PRIDEiQ62636.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009511; ENSRNOP00000009511; ENSRNOG00000007048.
GeneIDi171337.
KEGGirno:171337.
UCSCiRGD:620577. rat.

Organism-specific databases

CTDi5908.
RGDi620577. Rap1b.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00860000133678.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ62636.
KOiK07836.
OMAiRDSTHPR.
OrthoDBiEOG091G0OOG.
PhylomeDBiQ62636.
TreeFamiTF313014.

Enzyme and pathway databases

ReactomeiR-RNO-354192. Integrin alphaIIb beta3 signaling.
R-RNO-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-RNO-372708. p130Cas linkage to MAPK signaling for integrins.
R-RNO-392517. Rap1 signalling.
R-RNO-6798695. Neutrophil degranulation.
R-RNO-8875555. MET activates RAP1 and RAC1.

Miscellaneous databases

PROiQ62636.

Gene expression databases

BgeeiENSRNOG00000007048.
GenevisibleiQ62636. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAP1B_RAT
AccessioniPrimary (citable) accession number: Q62636
Secondary accession number(s): Q6J167
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: August 16, 2004
Last modified: November 30, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.