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Protein

Ras-related protein Rap-1b

Gene

Rap1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function (By similarity).By similarity

Enzyme regulationi

Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi116 – 1194GTPBy similarity
Nucleotide bindingi147 – 1493GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-354192. Integrin alphaIIb beta3 signaling.
R-RNO-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-RNO-372708. p130Cas linkage to MAPK signaling for integrins.
R-RNO-392517. Rap1 signalling.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-1b
Alternative name(s):
GTP-binding protein smg p21B
Gene namesi
Name:Rap1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620577. Rap1b.

Subcellular locationi

  • Cell membrane By similarity
  • Cytoplasmcytosol By similarity
  • Cell junction By similarity

  • Note: May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Ras-related protein Rap-1bPRO_0000030213Add
BLAST
Propeptidei182 – 1843Removed in mature formBy similarityPRO_0000030214

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei39 – 391ADP-ribosylserine; by botulinum toxinBy similarity
Modified residuei179 – 1791Phosphoserine; by PKABy similarity
Modified residuei181 – 1811Cysteine methyl esterBy similarity
Lipidationi181 – 1811S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

ADP-ribosylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ62636.
PRIDEiQ62636.

PTM databases

iPTMnetiQ62636.
PhosphoSiteiQ62636.

Expressioni

Gene expression databases

GenevisibleiQ62636. RN.

Interactioni

Subunit structurei

Interacts with SGSM1, SGSM2 and SGSM3. Interacts with KRIT1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ62636. 1 interaction.
MINTiMINT-4542540.
STRINGi10116.ENSRNOP00000009511.

Structurei

Secondary structure

1
184
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Helixi16 – 2510Combined sources
Beta strandi39 – 468Combined sources
Beta strandi49 – 579Combined sources
Helixi61 – 633Combined sources
Helixi68 – 747Combined sources
Beta strandi76 – 838Combined sources
Helixi87 – 10418Combined sources
Beta strandi111 – 1166Combined sources
Helixi121 – 1233Combined sources
Helixi128 – 13710Combined sources
Beta strandi143 – 1453Combined sources
Turni148 – 1514Combined sources
Helixi154 – 16512Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3X1WX-ray1.20A1-167[»]
3X1XX-ray1.00A1-167[»]
3X1YX-ray1.17A1-167[»]
3X1ZX-ray1.25A/B1-167[»]
ProteinModelPortaliQ62636.
SMRiQ62636. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 6743Interaction with KRIT1By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector regionCurated

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ62636.
KOiK07836.
OMAiRDSTHPR.
OrthoDBiEOG7QVM41.
PhylomeDBiQ62636.
TreeFamiTF313014.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ
60 70 80 90 100
CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI
110 120 130 140 150
LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWSN CAFLESSAKS
160 170 180
KINVNEIFYD LVRQINRKTP VPGKARKKSS CQLL
Length:184
Mass (Da):20,798
Last modified:August 16, 2004 - v2
Checksum:i37A76895E58DD80C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581T → A in AAA92787 (Ref. 1) Curated
Sequence conflicti75 – 751G → E in AAA92787 (Ref. 1) Curated
Sequence conflicti86 – 905AQSTF → TQIDC in AAA92787 (Ref. 1) Curated
Sequence conflicti95 – 951D → G in AAA92787 (Ref. 1) Curated
Sequence conflicti127 – 1271G → P in AAA92787 (Ref. 1) Curated
Sequence conflicti174 – 1752KA → NR in AAA92787 (Ref. 1) Curated
Sequence conflicti180 – 1801S → P in AAA92787 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07795 mRNA. Translation: AAA92787.1.
AY607847 mRNA. Translation: AAT37620.1.
BC081731 mRNA. Translation: AAH81731.1.
RefSeqiNP_599173.2. NM_134346.3.
XP_008763599.1. XM_008765377.1.
UniGeneiRn.95071.

Genome annotation databases

EnsembliENSRNOT00000009511; ENSRNOP00000009511; ENSRNOG00000007048.
GeneIDi171337.
KEGGirno:171337.
UCSCiRGD:620577. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07795 mRNA. Translation: AAA92787.1.
AY607847 mRNA. Translation: AAT37620.1.
BC081731 mRNA. Translation: AAH81731.1.
RefSeqiNP_599173.2. NM_134346.3.
XP_008763599.1. XM_008765377.1.
UniGeneiRn.95071.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3X1WX-ray1.20A1-167[»]
3X1XX-ray1.00A1-167[»]
3X1YX-ray1.17A1-167[»]
3X1ZX-ray1.25A/B1-167[»]
ProteinModelPortaliQ62636.
SMRiQ62636. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ62636. 1 interaction.
MINTiMINT-4542540.
STRINGi10116.ENSRNOP00000009511.

PTM databases

iPTMnetiQ62636.
PhosphoSiteiQ62636.

Proteomic databases

PaxDbiQ62636.
PRIDEiQ62636.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009511; ENSRNOP00000009511; ENSRNOG00000007048.
GeneIDi171337.
KEGGirno:171337.
UCSCiRGD:620577. rat.

Organism-specific databases

CTDi5908.
RGDi620577. Rap1b.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ62636.
KOiK07836.
OMAiRDSTHPR.
OrthoDBiEOG7QVM41.
PhylomeDBiQ62636.
TreeFamiTF313014.

Enzyme and pathway databases

ReactomeiR-RNO-354192. Integrin alphaIIb beta3 signaling.
R-RNO-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-RNO-372708. p130Cas linkage to MAPK signaling for integrins.
R-RNO-392517. Rap1 signalling.

Miscellaneous databases

NextBioi622102.
PROiQ62636.

Gene expression databases

GenevisibleiQ62636. RN.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of rat Ras like Rap1B cDNA."
    Rishi A.K., Gulamhussein A.I., Steele M.P.
    Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence of Rap1b from Wistar rat thyroid cells."
    Tsygankova O.M., Meinkoth J.L.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Thyroid.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiRAP1B_RAT
AccessioniPrimary (citable) accession number: Q62636
Secondary accession number(s): Q6J167
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: August 16, 2004
Last modified: May 11, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.