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Protein

Mucin-2

Gene

Muc2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer (By similarity).By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: RGD

GO - Biological processi

  • cellular response to tumor necrosis factor Source: RGD
  • response to hormone Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to transforming growth factor beta Source: RGD
  • response to vitamin A Source: RGD
Complete GO annotation...

Protein family/group databases

MEROPSiI08.954.

Names & Taxonomyi

Protein namesi
Recommended name:
Mucin-2
Short name:
MUC-2
Alternative name(s):
Intestinal mucin-2
Gene namesi
Name:Muc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3123. Muc2.

Subcellular locationi

  • Secreted

  • Note: In the intestine, secreted into the inner and outer mucus layers.By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • inner mucus layer Source: UniProtKB
  • mucus layer Source: RGD
  • nucleus Source: RGD
  • outer mucus layer Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001928221 – ›1513Mucin-2Add BLAST›1493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi160N-linked (GlcNAc...)Sequence analysis1
Glycosylationi420N-linked (GlcNAc...)Sequence analysis1
Glycosylationi667N-linked (GlcNAc...)Sequence analysis1
Glycosylationi767N-linked (GlcNAc...)Sequence analysis1
Glycosylationi837N-linked (GlcNAc...)Sequence analysis1
Glycosylationi892N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1136N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1151N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1212N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1243N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1350N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

O-glycosylated.By similarity
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.By similarity
May undergo autocatalytic cleavage in vivo triggered by the low pH of the late secretory pathway.By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ62635.

Expressioni

Tissue specificityi

Expressed in intestine and airway.

Interactioni

Subunit structurei

Homotrimer; disulfide-linked. Dimerizes in the endoplasmic reticulum via its C-terminal region and polymerizes via its N-terminal region by disulfide-linked trimerization. Interacts with FCGBP (By similarity). Interacts with AGR2; disulfide-linked (By similarity).By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: RGD

Structurei

3D structure databases

ProteinModelPortaliQ62635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 237VWFD 1PROSITE-ProRule annotationAdd BLAST205
Domaini292 – 348TILAdd BLAST57
Domaini350 – 410VWFCAdd BLAST61
Domaini387 – 601VWFD 2PROSITE-ProRule annotationAdd BLAST215
Domaini857 – 1062VWFD 3PROSITE-ProRule annotationAdd BLAST206
Repeati1392 – 14071Add BLAST16
Repeati1408 – 14232Add BLAST16
Repeati1424 – 14343Add BLAST11
Repeati1435 – 14454Add BLAST11
Repeati1446 – 14565Add BLAST11
Repeati1457 – 14676Add BLAST11
Repeati1468 – 14787Add BLAST11
Repeati1479 – 14898Add BLAST11
Repeati1490 – 15009Add BLAST11
Repeati1501 – 151110Add BLAST11
Repeati1512 – ›151311›2

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1392 – ›1513Approximate repeatsAdd BLAST›122

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1257 – 1292Thr-richAdd BLAST36
Compositional biasi1389 – 1512Ser-richAdd BLAST124

Sequence similaritiesi

Contains 1 VWFC domain.Curated
Contains 3 VWFD domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG004380.
InParanoidiQ62635.
PhylomeDBiQ62635.

Family and domain databases

InterProiIPR028580. MUC2.
IPR002919. TIL_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_dom.
IPR001846. VWF_type-D.
IPR025155. WxxW_domain.
[Graphical view]
PANTHERiPTHR11339:SF261. PTHR11339:SF261. 1 hit.
PfamiPF08742. C8. 3 hits.
PF13330. Mucin2_WxxW. 1 hit.
PF01826. TIL. 2 hits.
PF00094. VWD. 3 hits.
[Graphical view]
SMARTiSM00832. C8. 3 hits.
SM00215. VWC_out. 2 hits.
SM00216. VWD. 3 hits.
[Graphical view]
SUPFAMiSSF57567. SSF57567. 3 hits.
PROSITEiPS51233. VWFD. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLPLARLVA VCLVLALAKG LELQKEARSR NHVCSTWGDF HYKTFDGDVF
60 70 80 90 100
RFPGLCDYNF ASDCRDSYKE FAVHLKRGLD KAGGHSSIES VLITIKDDTI
110 120 130 140 150
YLTHKLAVVN GAMVSTPHYS SGLLIEKNDA YTKVYSRAGL SLMWNREDAL
160 170 180 190 200
MVELDGRFQN HTCGLCGDFN GMQANNEFLS DGIRFSAIEF GNMQKINKPE
210 220 230 240 250
VVCEDPEEVQ EPESCSEHRA ECERLLTSTA FEDCQARVPV ELYVLACMHD
260 270 280 290 300
RCQCPQGGAC ECSTLAEFSR QCSHAGGRPE NWRTASLCPK KCPGNMVYLE
310 320 330 340 350
SGSPWLDTCS HLEVSSLCEE HYMDGCFCPE GTVYDDITGS GCIPVSQCHC
360 370 380 390 400
KLHGHLYMPG QEITNDCEQC VCNAGRWMCK DLPCPETCAL EGGSHITTFD
410 420 430 440 450
GKKFTFHGDC YYVLTKTKYN DSYALLGELA SCGSTDKQTC LKTVVLLTDN
460 470 480 490 500
KKNVVAFKSG GSVLLNEMEV SLPHVAASFS IFKPSSYHIV VNTMFGLRLQ
510 520 530 540 550
IQLVPVMQLF VTLDQSAQGQ VQGLCGNFNG LESDDFMTSG GMVEATGAGF
560 570 580 590 600
ANTWKAQSSC HDKLDWLDDP CPLNIESANY AEHWCSLLKR SETPFARCHL
610 620 630 640 650
AVDPTEYYKR CKYDTCNCQN NEDCMCAALS SYARACAAKG VMLWGWRESV
660 670 680 690 700
CNKDVHACPS SQIFMYNLTT CQQTCRSISE GDTHCLKGFA PVEGCGCPDH
710 720 730 740 750
TFMDEKGRCV PLSKCSCYHH GLYLEAGDVI LRQEERCICR NGRLQCTQVK
760 770 780 790 800
LIGHTCLSPQ ILVDCNNLTA LAIREPRPTS CQTLVARYYH TECISGCVCP
810 820 830 840 850
DGLLDNGRGG CVVEDECPCI HNKQFYDSGK SIKLDCNNTC TCQKGRWECT
860 870 880 890 900
RYACHSTCSI YGSGHYITFD GKHYDFDGHC SYVAVQDYCG QNSTGSFSII
910 920 930 940 950
TENVPCGTTG VTCSKAIKIF IGGTELKLVD KHRVVKQLEE GHHVPFITRE
960 970 980 990 1000
VGLYLVVEVS SGIIVIWDKK TTIFIKLDPS YKGNVCGLCG NFDDQTKNDF
1010 1020 1030 1040 1050
TTRDHMVVAS ELDFGNSWKE ASTCPDVSHN PDPCSLNPHR RSWAEKQCSI
1060 1070 1080 1090 1100
IKSDVFLACH GKVDPTVFYD ACVHDSCSCD TGGDCECFCS AVASYAQECT
1110 1120 1130 1140 1150
KAEACVFWRT PDLCPVFCDY YNPPDECEWH YEPCGNRSFE TCRTLNGIHS
1160 1170 1180 1190 1200
NISVSYLEGC YPRCPEDRPI YDEDLKKCVS GDKCGCYIED TRYPPGGSVP
1210 1220 1230 1240 1250
TDEICMSCTC TNTSEIICRP DEGKIINQTQ DGIFCYWETC GSNGTVEKHF
1260 1270 1280 1290 1300
EICVSSTLSP TSMTSFTTTS TPISTTPIST TITTTSATAT TTVPCCFWSD
1310 1320 1330 1340 1350
WINNNHPTSG NGGDRENFEH VCSAPENIEC RAATDPKLDW TELGQKVQCN
1360 1370 1380 1390 1400
VSEGLICNNE DQYGTGQFEL CYDYEIRVNC CFPMEYCLST VSPTTSTPIS
1410 1420 1430 1440 1450
STPQPTSSPT TLPTTSPLTS SATSPTTSHI TSTVSPTTSP TTSTTSPTTS
1460 1470 1480 1490 1500
PTTSTTSPTT STTSPTPSPT TSTTSPTPSP TTSTTSPTPS PTTSTTSPTT
1510
SPITSPTTST TSP
Length:1,513
Mass (Da):166,038
Last modified:November 1, 1996 - v1
Checksum:i26109DCA1BE7D008
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei15131

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07615 mRNA. Translation: AAA21655.2.
PIRiA54895.
UniGeneiRn.217174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07615 mRNA. Translation: AAA21655.2.
PIRiA54895.
UniGeneiRn.217174.

3D structure databases

ProteinModelPortaliQ62635.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI08.954.

Proteomic databases

PRIDEiQ62635.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi3123. Muc2.

Phylogenomic databases

HOVERGENiHBG004380.
InParanoidiQ62635.
PhylomeDBiQ62635.

Family and domain databases

InterProiIPR028580. MUC2.
IPR002919. TIL_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_dom.
IPR001846. VWF_type-D.
IPR025155. WxxW_domain.
[Graphical view]
PANTHERiPTHR11339:SF261. PTHR11339:SF261. 1 hit.
PfamiPF08742. C8. 3 hits.
PF13330. Mucin2_WxxW. 1 hit.
PF01826. TIL. 2 hits.
PF00094. VWD. 3 hits.
[Graphical view]
SMARTiSM00832. C8. 3 hits.
SM00215. VWC_out. 2 hits.
SM00216. VWD. 3 hits.
[Graphical view]
SUPFAMiSSF57567. SSF57567. 3 hits.
PROSITEiPS51233. VWFD. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMUC2_RAT
AccessioniPrimary (citable) accession number: Q62635
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.