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Q62635 (MUC2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Mucin-2
Short name=MUC-2
Alternative name(s):
Intestinal mucin-2
Gene names
Name:Muc2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length1513 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer By similarity.

Subunit structure

Homotrimer; disulfide-linked. Dimerizes in the endoplasmic reticulum via its C-terminal region and polymerizes via its N-terminal region by disulfide-linked trimerization. Interacts with FCGBP By similarity. Interacts with AGR2; disulfide-linked By similarity.

Subcellular location

Secreted. Note: In the intestine, secreted into the inner and outer mucus layers By similarity.

Tissue specificity

Expressed in intestine and airway.

Post-translational modification

O-glycosylated By similarity.

May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria By similarity.

May undergo autocatalytic cleavage in vivo triggered by the low pH of the late secretory pathway By similarity.

Sequence similarities

Contains 1 TIL (trypsin inhibitory-like) domain.

Contains 1 VWFC domain.

Contains 3 VWFD domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – ›1513›1493Mucin-2
PRO_0000019282

Regions

Domain33 – 237205VWFD 1
Domain292 – 34857TIL
Domain350 – 41061VWFC
Domain387 – 601215VWFD 2
Domain857 – 1062206VWFD 3
Repeat1392 – 1407161
Repeat1408 – 1423162
Repeat1424 – 1434113
Repeat1435 – 1445114
Repeat1446 – 1456115
Repeat1457 – 1467116
Repeat1468 – 1478117
Repeat1479 – 1489118
Repeat1490 – 1500119
Repeat1501 – 15111110
Repeat1512 – ›1513›211
Region1392 – ›1513›122Approximate repeats
Compositional bias1257 – 129236Thr-rich
Compositional bias1389 – 1512124Ser-rich

Amino acid modifications

Glycosylation1601N-linked (GlcNAc...) Potential
Glycosylation4201N-linked (GlcNAc...) Potential
Glycosylation6671N-linked (GlcNAc...) Potential
Glycosylation7671N-linked (GlcNAc...) Potential
Glycosylation8371N-linked (GlcNAc...) Potential
Glycosylation8921N-linked (GlcNAc...) Potential
Glycosylation11361N-linked (GlcNAc...) Potential
Glycosylation11511N-linked (GlcNAc...) Potential
Glycosylation12121N-linked (GlcNAc...) Potential
Glycosylation12271N-linked (GlcNAc...) Potential
Glycosylation12431N-linked (GlcNAc...) Potential
Glycosylation13501N-linked (GlcNAc...) Potential

Experimental info

Non-terminal residue15131

Sequences

Sequence LengthMass (Da)Tools
Q62635 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 26109DCA1BE7D008

FASTA1,513166,038
        10         20         30         40         50         60 
MGLPLARLVA VCLVLALAKG LELQKEARSR NHVCSTWGDF HYKTFDGDVF RFPGLCDYNF 

        70         80         90        100        110        120 
ASDCRDSYKE FAVHLKRGLD KAGGHSSIES VLITIKDDTI YLTHKLAVVN GAMVSTPHYS 

       130        140        150        160        170        180 
SGLLIEKNDA YTKVYSRAGL SLMWNREDAL MVELDGRFQN HTCGLCGDFN GMQANNEFLS 

       190        200        210        220        230        240 
DGIRFSAIEF GNMQKINKPE VVCEDPEEVQ EPESCSEHRA ECERLLTSTA FEDCQARVPV 

       250        260        270        280        290        300 
ELYVLACMHD RCQCPQGGAC ECSTLAEFSR QCSHAGGRPE NWRTASLCPK KCPGNMVYLE 

       310        320        330        340        350        360 
SGSPWLDTCS HLEVSSLCEE HYMDGCFCPE GTVYDDITGS GCIPVSQCHC KLHGHLYMPG 

       370        380        390        400        410        420 
QEITNDCEQC VCNAGRWMCK DLPCPETCAL EGGSHITTFD GKKFTFHGDC YYVLTKTKYN 

       430        440        450        460        470        480 
DSYALLGELA SCGSTDKQTC LKTVVLLTDN KKNVVAFKSG GSVLLNEMEV SLPHVAASFS 

       490        500        510        520        530        540 
IFKPSSYHIV VNTMFGLRLQ IQLVPVMQLF VTLDQSAQGQ VQGLCGNFNG LESDDFMTSG 

       550        560        570        580        590        600 
GMVEATGAGF ANTWKAQSSC HDKLDWLDDP CPLNIESANY AEHWCSLLKR SETPFARCHL 

       610        620        630        640        650        660 
AVDPTEYYKR CKYDTCNCQN NEDCMCAALS SYARACAAKG VMLWGWRESV CNKDVHACPS 

       670        680        690        700        710        720 
SQIFMYNLTT CQQTCRSISE GDTHCLKGFA PVEGCGCPDH TFMDEKGRCV PLSKCSCYHH 

       730        740        750        760        770        780 
GLYLEAGDVI LRQEERCICR NGRLQCTQVK LIGHTCLSPQ ILVDCNNLTA LAIREPRPTS 

       790        800        810        820        830        840 
CQTLVARYYH TECISGCVCP DGLLDNGRGG CVVEDECPCI HNKQFYDSGK SIKLDCNNTC 

       850        860        870        880        890        900 
TCQKGRWECT RYACHSTCSI YGSGHYITFD GKHYDFDGHC SYVAVQDYCG QNSTGSFSII 

       910        920        930        940        950        960 
TENVPCGTTG VTCSKAIKIF IGGTELKLVD KHRVVKQLEE GHHVPFITRE VGLYLVVEVS 

       970        980        990       1000       1010       1020 
SGIIVIWDKK TTIFIKLDPS YKGNVCGLCG NFDDQTKNDF TTRDHMVVAS ELDFGNSWKE 

      1030       1040       1050       1060       1070       1080 
ASTCPDVSHN PDPCSLNPHR RSWAEKQCSI IKSDVFLACH GKVDPTVFYD ACVHDSCSCD 

      1090       1100       1110       1120       1130       1140 
TGGDCECFCS AVASYAQECT KAEACVFWRT PDLCPVFCDY YNPPDECEWH YEPCGNRSFE 

      1150       1160       1170       1180       1190       1200 
TCRTLNGIHS NISVSYLEGC YPRCPEDRPI YDEDLKKCVS GDKCGCYIED TRYPPGGSVP 

      1210       1220       1230       1240       1250       1260 
TDEICMSCTC TNTSEIICRP DEGKIINQTQ DGIFCYWETC GSNGTVEKHF EICVSSTLSP 

      1270       1280       1290       1300       1310       1320 
TSMTSFTTTS TPISTTPIST TITTTSATAT TTVPCCFWSD WINNNHPTSG NGGDRENFEH 

      1330       1340       1350       1360       1370       1380 
VCSAPENIEC RAATDPKLDW TELGQKVQCN VSEGLICNNE DQYGTGQFEL CYDYEIRVNC 

      1390       1400       1410       1420       1430       1440 
CFPMEYCLST VSPTTSTPIS STPQPTSSPT TLPTTSPLTS SATSPTTSHI TSTVSPTTSP 

      1450       1460       1470       1480       1490       1500 
TTSTTSPTTS PTTSTTSPTT STTSPTPSPT TSTTSPTPSP TTSTTSPTPS PTTSTTSPTT 

      1510 
SPITSPTTST TSP

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References

[1]"Molecular cloning of the amino-terminal region of a rat MUC 2 mucin gene homologue. Evidence for expression in both intestine and airway."
Ohmori H., Dohrman A.F., Gallup M., Tsuda T., Kai H., Gum J.R. Jr., Kim Y.S., Basbaum C.B.
J. Biol. Chem. 269:17833-17840(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Intestine.
[2]"Susceptibility of the cysteine-rich N-terminal and C-terminal ends of rat intestinal mucin Muc 2 to proteolytic cleavage."
Khatri I.A., Forstner G.G., Forstner J.F.
Biochem. J. 331:323-330(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-36.
Tissue: Intestinal epithelium.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U07615 mRNA. Translation: AAA21655.2.
IPIIPI00207066.
PIRA54895.

3D structure databases

ProteinModelPortalQ62635.
ModBaseSearch...

Proteomic databases

PRIDEQ62635.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD3123. Muc2.

Phylogenomic databases

HOVERGENHBG004380.

Gene expression databases

GenevestigatorQ62635.

Family and domain databases

InterProIPR002919. TIL_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
IPR025155. WxxW_domain.
[Graphical view]
PfamPF08742. C8. 3 hits.
PF13330. Mucin2_WxxW. 1 hit.
PF01826. TIL. 1 hit.
PF00094. VWD. 3 hits.
[Graphical view]
SMARTSM00832. C8. 3 hits.
SM00214. VWC. 1 hit.
SM00216. VWD. 3 hits.
[Graphical view]
SUPFAMSSF57567. Cysrich_TIL. 4 hits.
PROSITEPS01208. VWFC_1. False negative.
PS50184. VWFC_2. False negative.
PS51233. VWFD. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMUC2_RAT
AccessionPrimary (citable) accession number: Q62635
Entry history
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: November 1, 1996
Last modified: April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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