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Q62635

- MUC2_RAT

UniProt

Q62635 - MUC2_RAT

Protein

Mucin-2

Gene

Muc2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: RGD
    2. protein homodimerization activity Source: RGD

    GO - Biological processi

    1. cellular response to tumor necrosis factor Source: RGD
    2. response to hormone Source: RGD
    3. response to lipopolysaccharide Source: RGD
    4. response to vitamin A Source: RGD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mucin-2
    Short name:
    MUC-2
    Alternative name(s):
    Intestinal mucin-2
    Gene namesi
    Name:Muc2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3123. Muc2.

    Subcellular locationi

    Secreted
    Note: In the intestine, secreted into the inner and outer mucus layers.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. inner mucus layer Source: UniProtKB
    3. mucus layer Source: RGD
    4. nucleus Source: RGD
    5. outer mucus layer Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – ›1513›1493Mucin-2PRO_0000019282Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi420 – 4201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi667 – 6671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi767 – 7671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi837 – 8371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi892 – 8921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1136 – 11361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1151 – 11511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1212 – 12121N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1227 – 12271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1243 – 12431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1350 – 13501N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    O-glycosylated.By similarity
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.By similarity
    May undergo autocatalytic cleavage in vivo triggered by the low pH of the late secretory pathway.By similarity

    Keywords - PTMi

    Autocatalytic cleavage, Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ62635.

    Expressioni

    Tissue specificityi

    Expressed in intestine and airway.

    Gene expression databases

    GenevestigatoriQ62635.

    Interactioni

    Subunit structurei

    Homotrimer; disulfide-linked. Dimerizes in the endoplasmic reticulum via its C-terminal region and polymerizes via its N-terminal region by disulfide-linked trimerization. Interacts with FCGBP By similarity. Interacts with AGR2; disulfide-linked By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ62635.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 237205VWFD 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini292 – 34857TILAdd
    BLAST
    Domaini350 – 41061VWFCAdd
    BLAST
    Domaini387 – 601215VWFD 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini857 – 1062206VWFD 3PROSITE-ProRule annotationAdd
    BLAST
    Repeati1392 – 1407161Add
    BLAST
    Repeati1408 – 1423162Add
    BLAST
    Repeati1424 – 1434113Add
    BLAST
    Repeati1435 – 1445114Add
    BLAST
    Repeati1446 – 1456115Add
    BLAST
    Repeati1457 – 1467116Add
    BLAST
    Repeati1468 – 1478117Add
    BLAST
    Repeati1479 – 1489118Add
    BLAST
    Repeati1490 – 1500119Add
    BLAST
    Repeati1501 – 15111110Add
    BLAST
    Repeati1512 – ›1513›211

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1392 – ›1513›122Approximate repeatsAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1257 – 129236Thr-richAdd
    BLAST
    Compositional biasi1389 – 1512124Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 VWFC domain.Curated
    Contains 3 VWFD domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOVERGENiHBG004380.
    PhylomeDBiQ62635.

    Family and domain databases

    InterProiIPR028580. MUC2.
    IPR002919. TIL_dom.
    IPR014853. Unchr_dom_Cys-rich.
    IPR001007. VWF_C.
    IPR001846. VWF_type-D.
    IPR025155. WxxW_domain.
    [Graphical view]
    PANTHERiPTHR11339:SF261. PTHR11339:SF261. 1 hit.
    PfamiPF08742. C8. 3 hits.
    PF13330. Mucin2_WxxW. 1 hit.
    PF01826. TIL. 1 hit.
    PF00094. VWD. 3 hits.
    [Graphical view]
    SMARTiSM00832. C8. 3 hits.
    SM00214. VWC. 1 hit.
    SM00216. VWD. 3 hits.
    [Graphical view]
    SUPFAMiSSF57567. SSF57567. 3 hits.
    PROSITEiPS51233. VWFD. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q62635-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLPLARLVA VCLVLALAKG LELQKEARSR NHVCSTWGDF HYKTFDGDVF     50
    RFPGLCDYNF ASDCRDSYKE FAVHLKRGLD KAGGHSSIES VLITIKDDTI 100
    YLTHKLAVVN GAMVSTPHYS SGLLIEKNDA YTKVYSRAGL SLMWNREDAL 150
    MVELDGRFQN HTCGLCGDFN GMQANNEFLS DGIRFSAIEF GNMQKINKPE 200
    VVCEDPEEVQ EPESCSEHRA ECERLLTSTA FEDCQARVPV ELYVLACMHD 250
    RCQCPQGGAC ECSTLAEFSR QCSHAGGRPE NWRTASLCPK KCPGNMVYLE 300
    SGSPWLDTCS HLEVSSLCEE HYMDGCFCPE GTVYDDITGS GCIPVSQCHC 350
    KLHGHLYMPG QEITNDCEQC VCNAGRWMCK DLPCPETCAL EGGSHITTFD 400
    GKKFTFHGDC YYVLTKTKYN DSYALLGELA SCGSTDKQTC LKTVVLLTDN 450
    KKNVVAFKSG GSVLLNEMEV SLPHVAASFS IFKPSSYHIV VNTMFGLRLQ 500
    IQLVPVMQLF VTLDQSAQGQ VQGLCGNFNG LESDDFMTSG GMVEATGAGF 550
    ANTWKAQSSC HDKLDWLDDP CPLNIESANY AEHWCSLLKR SETPFARCHL 600
    AVDPTEYYKR CKYDTCNCQN NEDCMCAALS SYARACAAKG VMLWGWRESV 650
    CNKDVHACPS SQIFMYNLTT CQQTCRSISE GDTHCLKGFA PVEGCGCPDH 700
    TFMDEKGRCV PLSKCSCYHH GLYLEAGDVI LRQEERCICR NGRLQCTQVK 750
    LIGHTCLSPQ ILVDCNNLTA LAIREPRPTS CQTLVARYYH TECISGCVCP 800
    DGLLDNGRGG CVVEDECPCI HNKQFYDSGK SIKLDCNNTC TCQKGRWECT 850
    RYACHSTCSI YGSGHYITFD GKHYDFDGHC SYVAVQDYCG QNSTGSFSII 900
    TENVPCGTTG VTCSKAIKIF IGGTELKLVD KHRVVKQLEE GHHVPFITRE 950
    VGLYLVVEVS SGIIVIWDKK TTIFIKLDPS YKGNVCGLCG NFDDQTKNDF 1000
    TTRDHMVVAS ELDFGNSWKE ASTCPDVSHN PDPCSLNPHR RSWAEKQCSI 1050
    IKSDVFLACH GKVDPTVFYD ACVHDSCSCD TGGDCECFCS AVASYAQECT 1100
    KAEACVFWRT PDLCPVFCDY YNPPDECEWH YEPCGNRSFE TCRTLNGIHS 1150
    NISVSYLEGC YPRCPEDRPI YDEDLKKCVS GDKCGCYIED TRYPPGGSVP 1200
    TDEICMSCTC TNTSEIICRP DEGKIINQTQ DGIFCYWETC GSNGTVEKHF 1250
    EICVSSTLSP TSMTSFTTTS TPISTTPIST TITTTSATAT TTVPCCFWSD 1300
    WINNNHPTSG NGGDRENFEH VCSAPENIEC RAATDPKLDW TELGQKVQCN 1350
    VSEGLICNNE DQYGTGQFEL CYDYEIRVNC CFPMEYCLST VSPTTSTPIS 1400
    STPQPTSSPT TLPTTSPLTS SATSPTTSHI TSTVSPTTSP TTSTTSPTTS 1450
    PTTSTTSPTT STTSPTPSPT TSTTSPTPSP TTSTTSPTPS PTTSTTSPTT 1500
    SPITSPTTST TSP 1513
    Length:1,513
    Mass (Da):166,038
    Last modified:November 1, 1996 - v1
    Checksum:i26109DCA1BE7D008
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1513 – 15131

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07615 mRNA. Translation: AAA21655.2.
    PIRiA54895.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07615 mRNA. Translation: AAA21655.2 .
    PIRi A54895.

    3D structure databases

    ProteinModelPortali Q62635.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q62635.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    RGDi 3123. Muc2.

    Phylogenomic databases

    HOVERGENi HBG004380.
    PhylomeDBi Q62635.

    Gene expression databases

    Genevestigatori Q62635.

    Family and domain databases

    InterProi IPR028580. MUC2.
    IPR002919. TIL_dom.
    IPR014853. Unchr_dom_Cys-rich.
    IPR001007. VWF_C.
    IPR001846. VWF_type-D.
    IPR025155. WxxW_domain.
    [Graphical view ]
    PANTHERi PTHR11339:SF261. PTHR11339:SF261. 1 hit.
    Pfami PF08742. C8. 3 hits.
    PF13330. Mucin2_WxxW. 1 hit.
    PF01826. TIL. 1 hit.
    PF00094. VWD. 3 hits.
    [Graphical view ]
    SMARTi SM00832. C8. 3 hits.
    SM00214. VWC. 1 hit.
    SM00216. VWD. 3 hits.
    [Graphical view ]
    SUPFAMi SSF57567. SSF57567. 3 hits.
    PROSITEi PS51233. VWFD. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the amino-terminal region of a rat MUC 2 mucin gene homologue. Evidence for expression in both intestine and airway."
      Ohmori H., Dohrman A.F., Gallup M., Tsuda T., Kai H., Gum J.R. Jr., Kim Y.S., Basbaum C.B.
      J. Biol. Chem. 269:17833-17840(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Intestine.
    2. "Susceptibility of the cysteine-rich N-terminal and C-terminal ends of rat intestinal mucin Muc 2 to proteolytic cleavage."
      Khatri I.A., Forstner G.G., Forstner J.F.
      Biochem. J. 331:323-330(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-36.
      Tissue: Intestinal epithelium.

    Entry informationi

    Entry nameiMUC2_RAT
    AccessioniPrimary (citable) accession number: Q62635
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 17, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3