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Q62635

- MUC2_RAT

UniProt

Q62635 - MUC2_RAT

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Protein

Mucin-2

Gene

Muc2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer (By similarity).By similarity

GO - Molecular functioni

  1. protein homodimerization activity Source: RGD

GO - Biological processi

  1. cellular response to tumor necrosis factor Source: RGD
  2. response to hormone Source: RGD
  3. response to lipopolysaccharide Source: RGD
  4. response to transforming growth factor beta Source: RGD
  5. response to vitamin A Source: RGD
Complete GO annotation...

Protein family/group databases

MEROPSiI08.954.

Names & Taxonomyi

Protein namesi
Recommended name:
Mucin-2
Short name:
MUC-2
Alternative name(s):
Intestinal mucin-2
Gene namesi
Name:Muc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3123. Muc2.

Subcellular locationi

Secreted
Note: In the intestine, secreted into the inner and outer mucus layers.By similarity

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. inner mucus layer Source: UniProtKB
  3. mucus layer Source: RGD
  4. nucleus Source: RGD
  5. outer mucus layer Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – ›1513›1493Mucin-2PRO_0000019282Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi420 – 4201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi667 – 6671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi767 – 7671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi837 – 8371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi892 – 8921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1136 – 11361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1151 – 11511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1212 – 12121N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1227 – 12271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1243 – 12431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1350 – 13501N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

O-glycosylated.By similarity
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.By similarity
May undergo autocatalytic cleavage in vivo triggered by the low pH of the late secretory pathway.By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ62635.

Expressioni

Tissue specificityi

Expressed in intestine and airway.

Gene expression databases

GenevestigatoriQ62635.

Interactioni

Subunit structurei

Homotrimer; disulfide-linked. Dimerizes in the endoplasmic reticulum via its C-terminal region and polymerizes via its N-terminal region by disulfide-linked trimerization. Interacts with FCGBP (By similarity). Interacts with AGR2; disulfide-linked (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ62635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 237205VWFD 1PROSITE-ProRule annotationAdd
BLAST
Domaini292 – 34857TILAdd
BLAST
Domaini350 – 41061VWFCAdd
BLAST
Domaini387 – 601215VWFD 2PROSITE-ProRule annotationAdd
BLAST
Domaini857 – 1062206VWFD 3PROSITE-ProRule annotationAdd
BLAST
Repeati1392 – 1407161Add
BLAST
Repeati1408 – 1423162Add
BLAST
Repeati1424 – 1434113Add
BLAST
Repeati1435 – 1445114Add
BLAST
Repeati1446 – 1456115Add
BLAST
Repeati1457 – 1467116Add
BLAST
Repeati1468 – 1478117Add
BLAST
Repeati1479 – 1489118Add
BLAST
Repeati1490 – 1500119Add
BLAST
Repeati1501 – 15111110Add
BLAST
Repeati1512 – ›1513›211

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1392 – ›1513›122Approximate repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1257 – 129236Thr-richAdd
BLAST
Compositional biasi1389 – 1512124Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 VWFC domain.Curated
Contains 3 VWFD domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG004380.
InParanoidiQ62635.
PhylomeDBiQ62635.

Family and domain databases

InterProiIPR028580. MUC2.
IPR002919. TIL_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
IPR025155. WxxW_domain.
[Graphical view]
PANTHERiPTHR11339:SF261. PTHR11339:SF261. 1 hit.
PfamiPF08742. C8. 3 hits.
PF13330. Mucin2_WxxW. 1 hit.
PF01826. TIL. 1 hit.
PF00094. VWD. 3 hits.
[Graphical view]
SMARTiSM00832. C8. 3 hits.
SM00214. VWC. 1 hit.
SM00216. VWD. 3 hits.
[Graphical view]
SUPFAMiSSF57567. SSF57567. 3 hits.
PROSITEiPS51233. VWFD. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62635-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLPLARLVA VCLVLALAKG LELQKEARSR NHVCSTWGDF HYKTFDGDVF
60 70 80 90 100
RFPGLCDYNF ASDCRDSYKE FAVHLKRGLD KAGGHSSIES VLITIKDDTI
110 120 130 140 150
YLTHKLAVVN GAMVSTPHYS SGLLIEKNDA YTKVYSRAGL SLMWNREDAL
160 170 180 190 200
MVELDGRFQN HTCGLCGDFN GMQANNEFLS DGIRFSAIEF GNMQKINKPE
210 220 230 240 250
VVCEDPEEVQ EPESCSEHRA ECERLLTSTA FEDCQARVPV ELYVLACMHD
260 270 280 290 300
RCQCPQGGAC ECSTLAEFSR QCSHAGGRPE NWRTASLCPK KCPGNMVYLE
310 320 330 340 350
SGSPWLDTCS HLEVSSLCEE HYMDGCFCPE GTVYDDITGS GCIPVSQCHC
360 370 380 390 400
KLHGHLYMPG QEITNDCEQC VCNAGRWMCK DLPCPETCAL EGGSHITTFD
410 420 430 440 450
GKKFTFHGDC YYVLTKTKYN DSYALLGELA SCGSTDKQTC LKTVVLLTDN
460 470 480 490 500
KKNVVAFKSG GSVLLNEMEV SLPHVAASFS IFKPSSYHIV VNTMFGLRLQ
510 520 530 540 550
IQLVPVMQLF VTLDQSAQGQ VQGLCGNFNG LESDDFMTSG GMVEATGAGF
560 570 580 590 600
ANTWKAQSSC HDKLDWLDDP CPLNIESANY AEHWCSLLKR SETPFARCHL
610 620 630 640 650
AVDPTEYYKR CKYDTCNCQN NEDCMCAALS SYARACAAKG VMLWGWRESV
660 670 680 690 700
CNKDVHACPS SQIFMYNLTT CQQTCRSISE GDTHCLKGFA PVEGCGCPDH
710 720 730 740 750
TFMDEKGRCV PLSKCSCYHH GLYLEAGDVI LRQEERCICR NGRLQCTQVK
760 770 780 790 800
LIGHTCLSPQ ILVDCNNLTA LAIREPRPTS CQTLVARYYH TECISGCVCP
810 820 830 840 850
DGLLDNGRGG CVVEDECPCI HNKQFYDSGK SIKLDCNNTC TCQKGRWECT
860 870 880 890 900
RYACHSTCSI YGSGHYITFD GKHYDFDGHC SYVAVQDYCG QNSTGSFSII
910 920 930 940 950
TENVPCGTTG VTCSKAIKIF IGGTELKLVD KHRVVKQLEE GHHVPFITRE
960 970 980 990 1000
VGLYLVVEVS SGIIVIWDKK TTIFIKLDPS YKGNVCGLCG NFDDQTKNDF
1010 1020 1030 1040 1050
TTRDHMVVAS ELDFGNSWKE ASTCPDVSHN PDPCSLNPHR RSWAEKQCSI
1060 1070 1080 1090 1100
IKSDVFLACH GKVDPTVFYD ACVHDSCSCD TGGDCECFCS AVASYAQECT
1110 1120 1130 1140 1150
KAEACVFWRT PDLCPVFCDY YNPPDECEWH YEPCGNRSFE TCRTLNGIHS
1160 1170 1180 1190 1200
NISVSYLEGC YPRCPEDRPI YDEDLKKCVS GDKCGCYIED TRYPPGGSVP
1210 1220 1230 1240 1250
TDEICMSCTC TNTSEIICRP DEGKIINQTQ DGIFCYWETC GSNGTVEKHF
1260 1270 1280 1290 1300
EICVSSTLSP TSMTSFTTTS TPISTTPIST TITTTSATAT TTVPCCFWSD
1310 1320 1330 1340 1350
WINNNHPTSG NGGDRENFEH VCSAPENIEC RAATDPKLDW TELGQKVQCN
1360 1370 1380 1390 1400
VSEGLICNNE DQYGTGQFEL CYDYEIRVNC CFPMEYCLST VSPTTSTPIS
1410 1420 1430 1440 1450
STPQPTSSPT TLPTTSPLTS SATSPTTSHI TSTVSPTTSP TTSTTSPTTS
1460 1470 1480 1490 1500
PTTSTTSPTT STTSPTPSPT TSTTSPTPSP TTSTTSPTPS PTTSTTSPTT
1510
SPITSPTTST TSP
Length:1,513
Mass (Da):166,038
Last modified:November 1, 1996 - v1
Checksum:i26109DCA1BE7D008
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1513 – 15131

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07615 mRNA. Translation: AAA21655.2.
PIRiA54895.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07615 mRNA. Translation: AAA21655.2 .
PIRi A54895.

3D structure databases

ProteinModelPortali Q62635.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi I08.954.

Proteomic databases

PRIDEi Q62635.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

RGDi 3123. Muc2.

Phylogenomic databases

HOVERGENi HBG004380.
InParanoidi Q62635.
PhylomeDBi Q62635.

Gene expression databases

Genevestigatori Q62635.

Family and domain databases

InterProi IPR028580. MUC2.
IPR002919. TIL_dom.
IPR014853. Unchr_dom_Cys-rich.
IPR001007. VWF_C.
IPR001846. VWF_type-D.
IPR025155. WxxW_domain.
[Graphical view ]
PANTHERi PTHR11339:SF261. PTHR11339:SF261. 1 hit.
Pfami PF08742. C8. 3 hits.
PF13330. Mucin2_WxxW. 1 hit.
PF01826. TIL. 1 hit.
PF00094. VWD. 3 hits.
[Graphical view ]
SMARTi SM00832. C8. 3 hits.
SM00214. VWC. 1 hit.
SM00216. VWD. 3 hits.
[Graphical view ]
SUPFAMi SSF57567. SSF57567. 3 hits.
PROSITEi PS51233. VWFD. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the amino-terminal region of a rat MUC 2 mucin gene homologue. Evidence for expression in both intestine and airway."
    Ohmori H., Dohrman A.F., Gallup M., Tsuda T., Kai H., Gum J.R. Jr., Kim Y.S., Basbaum C.B.
    J. Biol. Chem. 269:17833-17840(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Intestine.
  2. "Susceptibility of the cysteine-rich N-terminal and C-terminal ends of rat intestinal mucin Muc 2 to proteolytic cleavage."
    Khatri I.A., Forstner G.G., Forstner J.F.
    Biochem. J. 331:323-330(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-36.
    Tissue: Intestinal epithelium.

Entry informationi

Entry nameiMUC2_RAT
AccessioniPrimary (citable) accession number: Q62635
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3