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Q62634 (VGLU1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicular glutamate transporter 1

Short name=VGluT1
Alternative name(s):
Brain-specific Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 7
Gene names
Name:Slc17a7
Synonyms:Bnpi, Vglut1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.10

Subunit structure

Interacts with SHANK3 By similarity.

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane. Membrane; Multi-pass membrane protein Potential. Cell junctionsynapsesynaptosome Ref.3 Ref.5 Ref.6 Ref.7 Ref.8.

Tissue specificity

Expressed in the cerebellum, cerberal cortex and hippocampus. Isoform 2 is expressed specifically in retina. Ref.1 Ref.2 Ref.5 Ref.6 Ref.7

Developmental stage

Expression of isoform 2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. Ref.2

Induction

Induced within cerebellar granule cells by exposure to N-methyl-D-aspartate (NMDA). Ref.1

Sequence similarities

Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Ontologies

Keywords
   Biological processIon transport
Neurotransmitter transport
Sodium transport
Symport
Transport
   Cellular componentCell junction
Cytoplasmic vesicle
Membrane
Synapse
Synaptosome
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandSodium
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processL-glutamate import

Inferred from electronic annotation. Source: Ensembl

long-term memory

Inferred from electronic annotation. Source: Ensembl

neurotransmitter transport

Inferred from electronic annotation. Source: UniProtKB-KW

phosphate ion transport

Inferred from direct assay Ref.1. Source: RGD

regulation of excitatory postsynaptic membrane potential

Inferred from electronic annotation. Source: Ensembl

sequestering of neurotransmitter

Inferred from electronic annotation. Source: Ensembl

sodium-dependent phosphate transport

Inferred from direct assay Ref.1. Source: GOC

synaptic transmission, glutamatergic

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

excitatory synapse

Inferred from direct assay PubMed 19730411PubMed 19914352. Source: BHF-UCL

integral component of membrane

Inferred from direct assay Ref.1. Source: RGD

intracellular organelle

Inferred from direct assay PubMed 17600303. Source: RGD

neuron projection

Inferred from electronic annotation. Source: UniProtKB-SubCell

presynaptic active zone

Inferred from electronic annotation. Source: Ensembl

synapse

Inferred from direct assay PubMed 15681343. Source: MGI

synaptic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioninorganic phosphate transmembrane transporter activity

Inferred from direct assay Ref.1. Source: RGD

sodium-dependent phosphate transmembrane transporter activity

Inferred from direct assay Ref.1. Source: RGD

sodium:inorganic phosphate symporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q62634-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q62634-2)

Also known as: Vglut1v;

The sequence of this isoform differs from the canonical sequence as follows:
     105-105: Q → QTPYRSVHKQEAVTPTVGDIQGGDTE

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560Vesicular glutamate transporter 1
PRO_0000331613

Regions

Topological domain1 – 6363Cytoplasmic Potential
Transmembrane64 – 8421Helical; Potential
Topological domain85 – 11632Extracellular Potential
Transmembrane117 – 13721Helical; Potential
Topological domain138 – 1403Cytoplasmic Potential
Transmembrane141 – 16121Helical; Potential
Topological domain162 – 1698Extracellular Potential
Transmembrane170 – 19021Helical; Potential
Topological domain191 – 20818Cytoplasmic Potential
Transmembrane209 – 22921Helical; Potential
Topological domain230 – 2367Extracellular Potential
Transmembrane237 – 25721Helical; Potential
Topological domain258 – 30245Cytoplasmic Potential
Transmembrane303 – 32321Helical; Potential
Topological domain324 – 34118Extracellular Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 37816Cytoplasmic Potential
Transmembrane379 – 39921Helical; Potential
Topological domain400 – 4012Extracellular Potential
Transmembrane402 – 42221Helical; Potential
Topological domain423 – 43513Cytoplasmic Potential
Transmembrane436 – 45621Helical; Potential
Topological domain457 – 46913Extracellular Potential
Transmembrane470 – 49021Helical; Potential
Topological domain491 – 56070Cytoplasmic Potential
Compositional bias530 – 55627Pro-rich

Natural variations

Alternative sequence1051Q → QTPYRSVHKQEAVTPTVGDI QGGDTE in isoform 2.
VSP_033264

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: F686889F606B8305

FASTA56061,665
        10         20         30         40         50         60 
MEFRQEEFRK LAGRALGRLH RLLEKRQEGA ETLELSADGR PVTTHTRDPP VVDCTCFGLP 

        70         80         90        100        110        120 
RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG HVVVQKAQFN WDPETVGLIH 

       130        140        150        160        170        180 
GSFFWGYIVT QIPGGFICQK FAANRVFGFA IVATSTLNML IPSAARVHYG CVIFVRILQG 

       190        200        210        220        230        240 
LVEGVTYPAC HGIWSKWAPP LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV 

       250        260        270        280        290        300 
YGSFGIFWYL FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPVTK FNTPWRRFFT 

       310        320        330        340        350        360 
SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV MTIIVPIGGQ 

       370        380        390        400        410        420 
IADFLRSRHI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS KGVAISFLVL AVGFSGFAIS 

       430        440        450        460        470        480 
GFNVNHLDIA PRYASILMGI SNGVGTLSGM VCPIIVGAMT KHKTREEWQY VFLIASLVHY 

       490        500        510        520        530        540 
GGVIFYGVFA SGEKQPWAEP EEMSEEKCGF VGHDQLAGSD ESEMEDEVEP PGAPPAPPPS 

       550        560 
YGATHSTVQP PRPPPPVRDY 

« Hide

Isoform 2 (Vglut1v) [UniParc].

Checksum: 58F4597D67C8422E
Show »

FASTA58564,333

References

[1]"Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter."
Ni B., Rosteck P.R. Jr., Nadi N.S., Paul S.M.
Proc. Natl. Acad. Sci. U.S.A. 91:5607-5611(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
[2]"Developmentally-regulated expression of tissue-specific splice variant of rat vesicular glutamate transporter 1 in retina and pineal gland."
Nogami H., Ogasawara K., Mimura Y., Mogi K., Shutoh F., Hisano S.
J. Neurochem. 99:142-153(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: Sprague-Dawley.
[3]"Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons."
Takamori S., Rhee J.S., Rosenmund C., Jahn R.
Nature 407:189-194(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 99-106; 197-203 AND 291-196, FUNCTION, SUBCELLULAR LOCATION.
[4]"Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter."
Bellocchio E.E., Reimer R.J., Fremeau R.T. Jr., Edwards R.H.
Science 289:957-960(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons."
Herzog E., Bellenchi G.C., Gras C., Bernard V., Ravassard P., Bedet C., Gasnier B., Giros B., El Mestikawy S.
J. Neurosci. 21:RC181-RC181(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[6]"Identification of differentiation-associated brain-specific phosphate transporter as a second vesicular glutamate transporter (VGLUT2)."
Takamori S., Rhee J.S., Rosenmund C., Jahn R.
J. Neurosci. 21:RC182-RC182(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"The expression of vesicular glutamate transporters defines two classes of excitatory synapse."
Fremeau R.T. Jr., Troyer M.D., Pahner I., Nygaard G.O., Tran C.H., Reimer R.J., Bellocchio E.E., Fortin D., Storm-Mathisen J., Edwards R.H.
Neuron 31:247-260(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]"The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone."
Gras C., Amilhon B., Lepicard E.M., Poirel O., Vinatier J., Herbin M., Dumas S., Tzavara E.T., Wade M.R., Nomikos G.G., Hanoun N., Saurini F., Kemel M.-L., Gasnier B., Giros B., Mestikawy S.E.
Nat. Neurosci. 11:292-300(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Molecular anatomy of a trafficking organelle."
Takamori S., Holt M., Stenius K., Lemke E.A., Groenborg M., Riedel D., Urlaub H., Schenck S., Bruegger B., Ringler P., Mueller S.A., Rammner B., Graeter F., Hub J.S., De Groot B.L., Mieskes G., Moriyama Y., Klingauf J. expand/collapse author list , Grubmueller H., Heuser J., Wieland F., Jahn R.
Cell 127:831-846(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[10]"Analysis of a vesicular glutamate transporter (VGLUT2) supports a cell-leakage mode in addition to vesicular packaging."
Mackenzie B., Illing A.C., Morris M.E.K., Varoqui H., Erickson J.D.
Neurochem. Res. 33:238-247(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U07609 mRNA. Translation: AAA19646.1.
EU253553 mRNA. Translation: ABX55782.1.
PIRI59302.
RefSeqNP_446311.1. NM_053859.2.
UniGeneRn.10267.

3D structure databases

ProteinModelPortalQ62634.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid250523. 2 interactions.
IntActQ62634. 5 interactions.
MINTMINT-4784487.

Protein family/group databases

TCDB2.A.1.14.13. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteQ62634.

Proteomic databases

PaxDbQ62634.
PRIDEQ62634.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028064; ENSRNOP00000028064; ENSRNOG00000020650. [Q62634-1]
ENSRNOT00000064184; ENSRNOP00000063604; ENSRNOG00000020650. [Q62634-2]
GeneID116638.
KEGGrno:116638.
UCSCRGD:620101. rat. [Q62634-1]

Organism-specific databases

CTD57030.
RGD620101. Slc17a7.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00630000089608.
HOGENOMHOG000230812.
HOVERGENHBG008834.
InParanoidQ62634.
KOK12302.
OMAMHGHVVI.
OrthoDBEOG789C9Z.
PhylomeDBQ62634.
TreeFamTF313535.

Gene expression databases

GenevestigatorQ62634.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio619351.
PROQ62634.

Entry information

Entry nameVGLU1_RAT
AccessionPrimary (citable) accession number: Q62634
Secondary accession number(s): A9LRT0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families