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Protein

Vesicular glutamate transporter 1

Gene

Slc17a7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.6 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_293445. Organic anion transporters.
REACT_319987. Glutamate Neurotransmitter Release Cycle.

Protein family/group databases

TCDBi2.A.1.14.13. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular glutamate transporter 1
Short name:
VGluT1
Alternative name(s):
Brain-specific Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 7
Gene namesi
Name:Slc17a7
Synonyms:Bnpi, Vglut1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620101. Slc17a7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6363CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei64 – 8421HelicalSequence AnalysisAdd
BLAST
Topological domaini85 – 11632ExtracellularSequence AnalysisAdd
BLAST
Transmembranei117 – 13721HelicalSequence AnalysisAdd
BLAST
Topological domaini138 – 1403CytoplasmicSequence Analysis
Transmembranei141 – 16121HelicalSequence AnalysisAdd
BLAST
Topological domaini162 – 1698ExtracellularSequence Analysis
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Topological domaini191 – 20818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei209 – 22921HelicalSequence AnalysisAdd
BLAST
Topological domaini230 – 2367ExtracellularSequence Analysis
Transmembranei237 – 25721HelicalSequence AnalysisAdd
BLAST
Topological domaini258 – 30245CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei303 – 32321HelicalSequence AnalysisAdd
BLAST
Topological domaini324 – 34118ExtracellularSequence AnalysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence AnalysisAdd
BLAST
Topological domaini363 – 37816CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei379 – 39921HelicalSequence AnalysisAdd
BLAST
Topological domaini400 – 4012ExtracellularSequence Analysis
Transmembranei402 – 42221HelicalSequence AnalysisAdd
BLAST
Topological domaini423 – 43513CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei436 – 45621HelicalSequence AnalysisAdd
BLAST
Topological domaini457 – 46913ExtracellularSequence AnalysisAdd
BLAST
Transmembranei470 – 49021HelicalSequence AnalysisAdd
BLAST
Topological domaini491 – 56070CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cerebellar mossy fiber Source: Ensembl
  • excitatory synapse Source: BHF-UCL
  • integral component of membrane Source: RGD
  • intracellular organelle Source: RGD
  • presynaptic active zone Source: MGI
  • synapse Source: MGI
  • synaptic vesicle lumen Source: GOC
  • synaptic vesicle membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Vesicular glutamate transporter 1PRO_0000331613Add
BLAST

Proteomic databases

PaxDbiQ62634.
PRIDEiQ62634.

PTM databases

PhosphoSiteiQ62634.

Expressioni

Tissue specificityi

Expressed in the cerebellum, cerberal cortex and hippocampus. Isoform 2 is expressed specifically in retina.5 Publications

Developmental stagei

Expression of isoform 2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina.1 Publication

Inductioni

Induced within cerebellar granule cells by exposure to N-methyl-D-aspartate (NMDA).1 Publication

Gene expression databases

ExpressionAtlasiQ62634. baseline and differential.
GenevisibleiQ62634. RN.

Interactioni

Subunit structurei

Interacts with SHANK3.By similarity

Protein-protein interaction databases

BioGridi250523. 2 interactions.
IntActiQ62634. 5 interactions.
MINTiMINT-4784487.
STRINGi10116.ENSRNOP00000063604.

Structurei

3D structure databases

ProteinModelPortaliQ62634.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi530 – 55627Pro-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00760000119079.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ62634.
KOiK12302.
OMAiVIIVRIF.
OrthoDBiEOG789C9Z.
PhylomeDBiQ62634.
TreeFamiTF313535.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62634-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEFRQEEFRK LAGRALGRLH RLLEKRQEGA ETLELSADGR PVTTHTRDPP
60 70 80 90 100
VVDCTCFGLP RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG
110 120 130 140 150
HVVVQKAQFN WDPETVGLIH GSFFWGYIVT QIPGGFICQK FAANRVFGFA
160 170 180 190 200
IVATSTLNML IPSAARVHYG CVIFVRILQG LVEGVTYPAC HGIWSKWAPP
210 220 230 240 250
LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV YGSFGIFWYL
260 270 280 290 300
FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPVTK FNTPWRRFFT
310 320 330 340 350
SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV
360 370 380 390 400
MTIIVPIGGQ IADFLRSRHI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS
410 420 430 440 450
KGVAISFLVL AVGFSGFAIS GFNVNHLDIA PRYASILMGI SNGVGTLSGM
460 470 480 490 500
VCPIIVGAMT KHKTREEWQY VFLIASLVHY GGVIFYGVFA SGEKQPWAEP
510 520 530 540 550
EEMSEEKCGF VGHDQLAGSD ESEMEDEVEP PGAPPAPPPS YGATHSTVQP
560
PRPPPPVRDY
Length:560
Mass (Da):61,665
Last modified:November 1, 1996 - v1
Checksum:iF686889F606B8305
GO
Isoform 2 (identifier: Q62634-2) [UniParc]FASTAAdd to basket

Also known as: Vglut1v

The sequence of this isoform differs from the canonical sequence as follows:
     105-105: Q → QTPYRSVHKQEAVTPTVGDIQGGDTE

Show »
Length:585
Mass (Da):64,333
Checksum:i58F4597D67C8422E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei105 – 1051Q → QTPYRSVHKQEAVTPTVGDI QGGDTE in isoform 2. 1 PublicationVSP_033264

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07609 mRNA. Translation: AAA19646.1.
EU253553 mRNA. Translation: ABX55782.1.
PIRiI59302.
RefSeqiNP_446311.1. NM_053859.2. [Q62634-1]
UniGeneiRn.10267.

Genome annotation databases

EnsembliENSRNOT00000028064; ENSRNOP00000028064; ENSRNOG00000020650. [Q62634-1]
ENSRNOT00000064184; ENSRNOP00000063604; ENSRNOG00000020650. [Q62634-2]
GeneIDi116638.
KEGGirno:116638.
UCSCiRGD:620101. rat. [Q62634-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07609 mRNA. Translation: AAA19646.1.
EU253553 mRNA. Translation: ABX55782.1.
PIRiI59302.
RefSeqiNP_446311.1. NM_053859.2. [Q62634-1]
UniGeneiRn.10267.

3D structure databases

ProteinModelPortaliQ62634.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250523. 2 interactions.
IntActiQ62634. 5 interactions.
MINTiMINT-4784487.
STRINGi10116.ENSRNOP00000063604.

Protein family/group databases

TCDBi2.A.1.14.13. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteiQ62634.

Proteomic databases

PaxDbiQ62634.
PRIDEiQ62634.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028064; ENSRNOP00000028064; ENSRNOG00000020650. [Q62634-1]
ENSRNOT00000064184; ENSRNOP00000063604; ENSRNOG00000020650. [Q62634-2]
GeneIDi116638.
KEGGirno:116638.
UCSCiRGD:620101. rat. [Q62634-1]

Organism-specific databases

CTDi57030.
RGDi620101. Slc17a7.

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00760000119079.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ62634.
KOiK12302.
OMAiVIIVRIF.
OrthoDBiEOG789C9Z.
PhylomeDBiQ62634.
TreeFamiTF313535.

Enzyme and pathway databases

ReactomeiREACT_293445. Organic anion transporters.
REACT_319987. Glutamate Neurotransmitter Release Cycle.

Miscellaneous databases

NextBioi619351.
PROiQ62634.

Gene expression databases

ExpressionAtlasiQ62634. baseline and differential.
GenevisibleiQ62634. RN.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter."
    Ni B., Rosteck P.R. Jr., Nadi N.S., Paul S.M.
    Proc. Natl. Acad. Sci. U.S.A. 91:5607-5611(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
  2. "Developmentally-regulated expression of tissue-specific splice variant of rat vesicular glutamate transporter 1 in retina and pineal gland."
    Nogami H., Ogasawara K., Mimura Y., Mogi K., Shutoh F., Hisano S.
    J. Neurochem. 99:142-153(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Sprague-Dawley.
  3. "Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons."
    Takamori S., Rhee J.S., Rosenmund C., Jahn R.
    Nature 407:189-194(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 99-106; 197-203 AND 291-196, FUNCTION, SUBCELLULAR LOCATION.
  4. "Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter."
    Bellocchio E.E., Reimer R.J., Fremeau R.T. Jr., Edwards R.H.
    Science 289:957-960(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons."
    Herzog E., Bellenchi G.C., Gras C., Bernard V., Ravassard P., Bedet C., Gasnier B., Giros B., El Mestikawy S.
    J. Neurosci. 21:RC181-RC181(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Identification of differentiation-associated brain-specific phosphate transporter as a second vesicular glutamate transporter (VGLUT2)."
    Takamori S., Rhee J.S., Rosenmund C., Jahn R.
    J. Neurosci. 21:RC182-RC182(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "The expression of vesicular glutamate transporters defines two classes of excitatory synapse."
    Fremeau R.T. Jr., Troyer M.D., Pahner I., Nygaard G.O., Tran C.H., Reimer R.J., Bellocchio E.E., Fortin D., Storm-Mathisen J., Edwards R.H.
    Neuron 31:247-260(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. Cited for: SUBCELLULAR LOCATION.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  10. "Analysis of a vesicular glutamate transporter (VGLUT2) supports a cell-leakage mode in addition to vesicular packaging."
    Mackenzie B., Illing A.C., Morris M.E.K., Varoqui H., Erickson J.D.
    Neurochem. Res. 33:238-247(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiVGLU1_RAT
AccessioniPrimary (citable) accession number: Q62634
Secondary accession number(s): A9LRT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 1, 1996
Last modified: July 22, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.