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Q62634

- VGLU1_RAT

UniProt

Q62634 - VGLU1_RAT

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Protein

Vesicular glutamate transporter 1

Gene
Slc17a7, Bnpi, Vglut1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.6 Publications

GO - Molecular functioni

  1. inorganic phosphate transmembrane transporter activity Source: RGD
  2. sodium:inorganic phosphate symporter activity Source: Ensembl
  3. sodium-dependent phosphate transmembrane transporter activity Source: RGD

GO - Biological processi

  1. L-glutamate import Source: Ensembl
  2. long-term memory Source: Ensembl
  3. neurotransmitter transport Source: UniProtKB-KW
  4. phosphate ion transport Source: RGD
  5. regulation of excitatory postsynaptic membrane potential Source: Ensembl
  6. sequestering of neurotransmitter Source: Ensembl
  7. sodium-dependent phosphate transport Source: GOC
  8. synaptic transmission, glutamatergic Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_227883. Glutamate Neurotransmitter Release Cycle.

Protein family/group databases

TCDBi2.A.1.14.13. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular glutamate transporter 1
Short name:
VGluT1
Alternative name(s):
Brain-specific Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 7
Gene namesi
Name:Slc17a7
Synonyms:Bnpi, Vglut1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi620101. Slc17a7.

Subcellular locationi

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane. Membrane; Multi-pass membrane protein Reviewed prediction. Cell junctionsynapsesynaptosome 5 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6363Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei64 – 8421Helical; Reviewed predictionAdd
BLAST
Topological domaini85 – 11632Extracellular Reviewed predictionAdd
BLAST
Transmembranei117 – 13721Helical; Reviewed predictionAdd
BLAST
Topological domaini138 – 1403Cytoplasmic Reviewed prediction
Transmembranei141 – 16121Helical; Reviewed predictionAdd
BLAST
Topological domaini162 – 1698Extracellular Reviewed prediction
Transmembranei170 – 19021Helical; Reviewed predictionAdd
BLAST
Topological domaini191 – 20818Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei209 – 22921Helical; Reviewed predictionAdd
BLAST
Topological domaini230 – 2367Extracellular Reviewed prediction
Transmembranei237 – 25721Helical; Reviewed predictionAdd
BLAST
Topological domaini258 – 30245Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei303 – 32321Helical; Reviewed predictionAdd
BLAST
Topological domaini324 – 34118Extracellular Reviewed predictionAdd
BLAST
Transmembranei342 – 36221Helical; Reviewed predictionAdd
BLAST
Topological domaini363 – 37816Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei379 – 39921Helical; Reviewed predictionAdd
BLAST
Topological domaini400 – 4012Extracellular Reviewed prediction
Transmembranei402 – 42221Helical; Reviewed predictionAdd
BLAST
Topological domaini423 – 43513Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei436 – 45621Helical; Reviewed predictionAdd
BLAST
Topological domaini457 – 46913Extracellular Reviewed predictionAdd
BLAST
Transmembranei470 – 49021Helical; Reviewed predictionAdd
BLAST
Topological domaini491 – 56070Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. excitatory synapse Source: BHF-UCL
  3. integral component of membrane Source: RGD
  4. intracellular organelle Source: RGD
  5. neuron projection Source: UniProtKB-SubCell
  6. presynaptic active zone Source: Ensembl
  7. synapse Source: MGI
  8. synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Vesicular glutamate transporter 1PRO_0000331613Add
BLAST

Proteomic databases

PaxDbiQ62634.
PRIDEiQ62634.

PTM databases

PhosphoSiteiQ62634.

Expressioni

Tissue specificityi

Expressed in the cerebellum, cerberal cortex and hippocampus. Isoform 2 is expressed specifically in retina.5 Publications

Developmental stagei

Expression of isoform 2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina.1 Publication

Inductioni

Induced within cerebellar granule cells by exposure to N-methyl-D-aspartate (NMDA).1 Publication

Gene expression databases

GenevestigatoriQ62634.

Interactioni

Subunit structurei

Interacts with SHANK3 By similarity.

Protein-protein interaction databases

BioGridi250523. 2 interactions.
IntActiQ62634. 5 interactions.
MINTiMINT-4784487.

Structurei

3D structure databases

ProteinModelPortaliQ62634.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi530 – 55627Pro-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00630000089608.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ62634.
KOiK12302.
OMAiMHGHVVI.
OrthoDBiEOG789C9Z.
PhylomeDBiQ62634.
TreeFamiTF313535.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q62634-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEFRQEEFRK LAGRALGRLH RLLEKRQEGA ETLELSADGR PVTTHTRDPP    50
VVDCTCFGLP RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG 100
HVVVQKAQFN WDPETVGLIH GSFFWGYIVT QIPGGFICQK FAANRVFGFA 150
IVATSTLNML IPSAARVHYG CVIFVRILQG LVEGVTYPAC HGIWSKWAPP 200
LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV YGSFGIFWYL 250
FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPVTK FNTPWRRFFT 300
SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV 350
MTIIVPIGGQ IADFLRSRHI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS 400
KGVAISFLVL AVGFSGFAIS GFNVNHLDIA PRYASILMGI SNGVGTLSGM 450
VCPIIVGAMT KHKTREEWQY VFLIASLVHY GGVIFYGVFA SGEKQPWAEP 500
EEMSEEKCGF VGHDQLAGSD ESEMEDEVEP PGAPPAPPPS YGATHSTVQP 550
PRPPPPVRDY 560
Length:560
Mass (Da):61,665
Last modified:November 1, 1996 - v1
Checksum:iF686889F606B8305
GO
Isoform 2 (identifier: Q62634-2) [UniParc]FASTAAdd to Basket

Also known as: Vglut1v

The sequence of this isoform differs from the canonical sequence as follows:
     105-105: Q → QTPYRSVHKQEAVTPTVGDIQGGDTE

Show »
Length:585
Mass (Da):64,333
Checksum:i58F4597D67C8422E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei105 – 1051Q → QTPYRSVHKQEAVTPTVGDI QGGDTE in isoform 2. VSP_033264

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U07609 mRNA. Translation: AAA19646.1.
EU253553 mRNA. Translation: ABX55782.1.
PIRiI59302.
RefSeqiNP_446311.1. NM_053859.2. [Q62634-1]
UniGeneiRn.10267.

Genome annotation databases

EnsembliENSRNOT00000028064; ENSRNOP00000028064; ENSRNOG00000020650. [Q62634-1]
ENSRNOT00000064184; ENSRNOP00000063604; ENSRNOG00000020650. [Q62634-2]
GeneIDi116638.
KEGGirno:116638.
UCSCiRGD:620101. rat. [Q62634-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U07609 mRNA. Translation: AAA19646.1 .
EU253553 mRNA. Translation: ABX55782.1 .
PIRi I59302.
RefSeqi NP_446311.1. NM_053859.2. [Q62634-1 ]
UniGenei Rn.10267.

3D structure databases

ProteinModelPortali Q62634.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250523. 2 interactions.
IntActi Q62634. 5 interactions.
MINTi MINT-4784487.

Protein family/group databases

TCDBi 2.A.1.14.13. the major facilitator superfamily (mfs).

PTM databases

PhosphoSitei Q62634.

Proteomic databases

PaxDbi Q62634.
PRIDEi Q62634.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000028064 ; ENSRNOP00000028064 ; ENSRNOG00000020650 . [Q62634-1 ]
ENSRNOT00000064184 ; ENSRNOP00000063604 ; ENSRNOG00000020650 . [Q62634-2 ]
GeneIDi 116638.
KEGGi rno:116638.
UCSCi RGD:620101. rat. [Q62634-1 ]

Organism-specific databases

CTDi 57030.
RGDi 620101. Slc17a7.

Phylogenomic databases

eggNOGi COG0477.
GeneTreei ENSGT00630000089608.
HOGENOMi HOG000230812.
HOVERGENi HBG008834.
InParanoidi Q62634.
KOi K12302.
OMAi MHGHVVI.
OrthoDBi EOG789C9Z.
PhylomeDBi Q62634.
TreeFami TF313535.

Enzyme and pathway databases

Reactomei REACT_227883. Glutamate Neurotransmitter Release Cycle.

Miscellaneous databases

NextBioi 619351.
PROi Q62634.

Gene expression databases

Genevestigatori Q62634.

Family and domain databases

InterProi IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter."
    Ni B., Rosteck P.R. Jr., Nadi N.S., Paul S.M.
    Proc. Natl. Acad. Sci. U.S.A. 91:5607-5611(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
  2. "Developmentally-regulated expression of tissue-specific splice variant of rat vesicular glutamate transporter 1 in retina and pineal gland."
    Nogami H., Ogasawara K., Mimura Y., Mogi K., Shutoh F., Hisano S.
    J. Neurochem. 99:142-153(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Sprague-Dawley.
  3. "Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons."
    Takamori S., Rhee J.S., Rosenmund C., Jahn R.
    Nature 407:189-194(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 99-106; 197-203 AND 291-196, FUNCTION, SUBCELLULAR LOCATION.
  4. "Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter."
    Bellocchio E.E., Reimer R.J., Fremeau R.T. Jr., Edwards R.H.
    Science 289:957-960(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons."
    Herzog E., Bellenchi G.C., Gras C., Bernard V., Ravassard P., Bedet C., Gasnier B., Giros B., El Mestikawy S.
    J. Neurosci. 21:RC181-RC181(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Identification of differentiation-associated brain-specific phosphate transporter as a second vesicular glutamate transporter (VGLUT2)."
    Takamori S., Rhee J.S., Rosenmund C., Jahn R.
    J. Neurosci. 21:RC182-RC182(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "The expression of vesicular glutamate transporters defines two classes of excitatory synapse."
    Fremeau R.T. Jr., Troyer M.D., Pahner I., Nygaard G.O., Tran C.H., Reimer R.J., Bellocchio E.E., Fortin D., Storm-Mathisen J., Edwards R.H.
    Neuron 31:247-260(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. Cited for: SUBCELLULAR LOCATION.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
  10. "Analysis of a vesicular glutamate transporter (VGLUT2) supports a cell-leakage mode in addition to vesicular packaging."
    Mackenzie B., Illing A.C., Morris M.E.K., Varoqui H., Erickson J.D.
    Neurochem. Res. 33:238-247(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiVGLU1_RAT
AccessioniPrimary (citable) accession number: Q62634
Secondary accession number(s): A9LRT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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