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Q62634

- VGLU1_RAT

UniProt

Q62634 - VGLU1_RAT

Protein

Vesicular glutamate transporter 1

Gene

Slc17a7

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.6 Publications

    GO - Molecular functioni

    1. inorganic phosphate transmembrane transporter activity Source: RGD
    2. sodium:inorganic phosphate symporter activity Source: Ensembl
    3. sodium-dependent phosphate transmembrane transporter activity Source: RGD

    GO - Biological processi

    1. L-glutamate import Source: Ensembl
    2. long-term memory Source: Ensembl
    3. neurotransmitter transport Source: UniProtKB-KW
    4. phosphate ion transport Source: RGD
    5. regulation of excitatory postsynaptic membrane potential Source: Ensembl
    6. sequestering of neurotransmitter Source: Ensembl
    7. sodium-dependent phosphate transport Source: GOC
    8. synaptic transmission, glutamatergic Source: Ensembl

    Keywords - Biological processi

    Ion transport, Neurotransmitter transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Enzyme and pathway databases

    ReactomeiREACT_227883. Glutamate Neurotransmitter Release Cycle.

    Protein family/group databases

    TCDBi2.A.1.14.13. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicular glutamate transporter 1
    Short name:
    VGluT1
    Alternative name(s):
    Brain-specific Na(+)-dependent inorganic phosphate cotransporter
    Solute carrier family 17 member 7
    Gene namesi
    Name:Slc17a7
    Synonyms:Bnpi, Vglut1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi620101. Slc17a7.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. excitatory synapse Source: BHF-UCL
    3. integral component of membrane Source: RGD
    4. intracellular organelle Source: RGD
    5. neuron projection Source: UniProtKB-SubCell
    6. presynaptic active zone Source: Ensembl
    7. synapse Source: MGI
    8. synaptic vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 560560Vesicular glutamate transporter 1PRO_0000331613Add
    BLAST

    Proteomic databases

    PaxDbiQ62634.
    PRIDEiQ62634.

    PTM databases

    PhosphoSiteiQ62634.

    Expressioni

    Tissue specificityi

    Expressed in the cerebellum, cerberal cortex and hippocampus. Isoform 2 is expressed specifically in retina.5 Publications

    Developmental stagei

    Expression of isoform 2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina.1 Publication

    Inductioni

    Induced within cerebellar granule cells by exposure to N-methyl-D-aspartate (NMDA).1 Publication

    Gene expression databases

    GenevestigatoriQ62634.

    Interactioni

    Subunit structurei

    Interacts with SHANK3.By similarity

    Protein-protein interaction databases

    BioGridi250523. 2 interactions.
    IntActiQ62634. 5 interactions.
    MINTiMINT-4784487.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62634.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6363CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini85 – 11632ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini138 – 1403CytoplasmicSequence Analysis
    Topological domaini162 – 1698ExtracellularSequence Analysis
    Topological domaini191 – 20818CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini230 – 2367ExtracellularSequence Analysis
    Topological domaini258 – 30245CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini324 – 34118ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini363 – 37816CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini400 – 4012ExtracellularSequence Analysis
    Topological domaini423 – 43513CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini457 – 46913ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini491 – 56070CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei64 – 8421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei117 – 13721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei141 – 16121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei209 – 22921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei237 – 25721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei303 – 32321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei342 – 36221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei379 – 39921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei402 – 42221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei436 – 45621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei470 – 49021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi530 – 55627Pro-richAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00630000089608.
    HOGENOMiHOG000230812.
    HOVERGENiHBG008834.
    InParanoidiQ62634.
    KOiK12302.
    OMAiMHGHVVI.
    OrthoDBiEOG789C9Z.
    PhylomeDBiQ62634.
    TreeFamiTF313535.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q62634-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEFRQEEFRK LAGRALGRLH RLLEKRQEGA ETLELSADGR PVTTHTRDPP    50
    VVDCTCFGLP RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG 100
    HVVVQKAQFN WDPETVGLIH GSFFWGYIVT QIPGGFICQK FAANRVFGFA 150
    IVATSTLNML IPSAARVHYG CVIFVRILQG LVEGVTYPAC HGIWSKWAPP 200
    LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV YGSFGIFWYL 250
    FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPVTK FNTPWRRFFT 300
    SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV 350
    MTIIVPIGGQ IADFLRSRHI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS 400
    KGVAISFLVL AVGFSGFAIS GFNVNHLDIA PRYASILMGI SNGVGTLSGM 450
    VCPIIVGAMT KHKTREEWQY VFLIASLVHY GGVIFYGVFA SGEKQPWAEP 500
    EEMSEEKCGF VGHDQLAGSD ESEMEDEVEP PGAPPAPPPS YGATHSTVQP 550
    PRPPPPVRDY 560
    Length:560
    Mass (Da):61,665
    Last modified:November 1, 1996 - v1
    Checksum:iF686889F606B8305
    GO
    Isoform 2 (identifier: Q62634-2) [UniParc]FASTAAdd to Basket

    Also known as: Vglut1v

    The sequence of this isoform differs from the canonical sequence as follows:
         105-105: Q → QTPYRSVHKQEAVTPTVGDIQGGDTE

    Show »
    Length:585
    Mass (Da):64,333
    Checksum:i58F4597D67C8422E
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei105 – 1051Q → QTPYRSVHKQEAVTPTVGDI QGGDTE in isoform 2. 1 PublicationVSP_033264

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07609 mRNA. Translation: AAA19646.1.
    EU253553 mRNA. Translation: ABX55782.1.
    PIRiI59302.
    RefSeqiNP_446311.1. NM_053859.2. [Q62634-1]
    UniGeneiRn.10267.

    Genome annotation databases

    EnsembliENSRNOT00000028064; ENSRNOP00000028064; ENSRNOG00000020650. [Q62634-1]
    ENSRNOT00000064184; ENSRNOP00000063604; ENSRNOG00000020650. [Q62634-2]
    GeneIDi116638.
    KEGGirno:116638.
    UCSCiRGD:620101. rat. [Q62634-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U07609 mRNA. Translation: AAA19646.1 .
    EU253553 mRNA. Translation: ABX55782.1 .
    PIRi I59302.
    RefSeqi NP_446311.1. NM_053859.2. [Q62634-1 ]
    UniGenei Rn.10267.

    3D structure databases

    ProteinModelPortali Q62634.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250523. 2 interactions.
    IntActi Q62634. 5 interactions.
    MINTi MINT-4784487.

    Protein family/group databases

    TCDBi 2.A.1.14.13. the major facilitator superfamily (mfs).

    PTM databases

    PhosphoSitei Q62634.

    Proteomic databases

    PaxDbi Q62634.
    PRIDEi Q62634.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000028064 ; ENSRNOP00000028064 ; ENSRNOG00000020650 . [Q62634-1 ]
    ENSRNOT00000064184 ; ENSRNOP00000063604 ; ENSRNOG00000020650 . [Q62634-2 ]
    GeneIDi 116638.
    KEGGi rno:116638.
    UCSCi RGD:620101. rat. [Q62634-1 ]

    Organism-specific databases

    CTDi 57030.
    RGDi 620101. Slc17a7.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00630000089608.
    HOGENOMi HOG000230812.
    HOVERGENi HBG008834.
    InParanoidi Q62634.
    KOi K12302.
    OMAi MHGHVVI.
    OrthoDBi EOG789C9Z.
    PhylomeDBi Q62634.
    TreeFami TF313535.

    Enzyme and pathway databases

    Reactomei REACT_227883. Glutamate Neurotransmitter Release Cycle.

    Miscellaneous databases

    NextBioi 619351.
    PROi Q62634.

    Gene expression databases

    Genevestigatori Q62634.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter."
      Ni B., Rosteck P.R. Jr., Nadi N.S., Paul S.M.
      Proc. Natl. Acad. Sci. U.S.A. 91:5607-5611(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    2. "Developmentally-regulated expression of tissue-specific splice variant of rat vesicular glutamate transporter 1 in retina and pineal gland."
      Nogami H., Ogasawara K., Mimura Y., Mogi K., Shutoh F., Hisano S.
      J. Neurochem. 99:142-153(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: Sprague-Dawley.
    3. "Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons."
      Takamori S., Rhee J.S., Rosenmund C., Jahn R.
      Nature 407:189-194(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 99-106; 197-203 AND 291-196, FUNCTION, SUBCELLULAR LOCATION.
    4. "Uptake of glutamate into synaptic vesicles by an inorganic phosphate transporter."
      Bellocchio E.E., Reimer R.J., Fremeau R.T. Jr., Edwards R.H.
      Science 289:957-960(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons."
      Herzog E., Bellenchi G.C., Gras C., Bernard V., Ravassard P., Bedet C., Gasnier B., Giros B., El Mestikawy S.
      J. Neurosci. 21:RC181-RC181(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    6. "Identification of differentiation-associated brain-specific phosphate transporter as a second vesicular glutamate transporter (VGLUT2)."
      Takamori S., Rhee J.S., Rosenmund C., Jahn R.
      J. Neurosci. 21:RC182-RC182(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    7. "The expression of vesicular glutamate transporters defines two classes of excitatory synapse."
      Fremeau R.T. Jr., Troyer M.D., Pahner I., Nygaard G.O., Tran C.H., Reimer R.J., Bellocchio E.E., Fortin D., Storm-Mathisen J., Edwards R.H.
      Neuron 31:247-260(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    8. Cited for: SUBCELLULAR LOCATION.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    10. "Analysis of a vesicular glutamate transporter (VGLUT2) supports a cell-leakage mode in addition to vesicular packaging."
      Mackenzie B., Illing A.C., Morris M.E.K., Varoqui H., Erickson J.D.
      Neurochem. Res. 33:238-247(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiVGLU1_RAT
    AccessioniPrimary (citable) accession number: Q62634
    Secondary accession number(s): A9LRT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3