Reviewed, UniProtKB/Swiss-Prot Q62625 (MLP3B_RAT)

Names and origin

Protein names

Recommended name:
    Microtubule-associated proteins 1A/1B light chain 3B
Alternative name(s):
    Microtubule-associated protein 1 light chain 3 beta
    MAP1A/1B light chain 3 B
    MAP1A/MAP1B LC3 B
    MAP1 light chain 3-like protein 2
    Autophagy-related protein LC3 B
    Autophagy-related ubiquitin-like modifier LC3 B

Gene names

Name:	Map1lc3b
Synonyms:	Map1alc3, Map1lc3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Subunit structure	3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins.
Subcellular location	Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Note= LC3-II binds to the autophagic membranes.
Tissue specificity	Abundant only in neurons. Detected in testes.
Post-translational modification	The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
Sequence similarities	Belongs to the MAP1 LC3 family.

Ontologies

Keywords
Biological process	Autophagy Ubl conjugation pathway
Cellular component	Cytoplasm Cytoplasmic vesicle Membrane Microtubule
Coding sequence diversity	Alternative splicing
PTM	Lipoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	autophagic vacuole membrane Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q62625-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q62625-2)

The sequence of this isoform differs from the canonical sequence as follows:
     126-142: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 120

119

Microtubule-associated proteins 1A/1B light chain 3B

Propeptide

121 – 142

22

Removed in mature form Probable

Amino acid modifications

Lipidation

120

1

Phosphatidylethanolamine amidated glycine By similarity

Natural variations

Alternative sequence

126 – 142

17

Missing in isoform 2.

Experimental info

Mutagenesis

120

1

G → A: No processing of precursor

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

142

Helix Strand Turn

Details...

Helix

7 – 10

4

Helix

13 – 26

14

Beta strand

30 – 37

8

Beta strand

50 – 55

6

Helix

60 – 70

11

Beta strand

80 – 83

4

Beta strand

91 – 94

4

Helix

95 – 102

8

Beta strand

109 – 115

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D07E9D063C125E32	FASTA	142	16,394
	10 20 30 40 50 60 MPSEKTFKQR RSFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 70 80 90 100 110 120 SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SERDEDGFLY MVYASQETFG 130 140 TALAVTYMSA LKATATGREP CL
	Isoform 2 [UniParc]. Checksum: 520E28B28163FA8D		125	14,599
	10 20 30 40 50 60 MPSEKTFKQR RSFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 70 80 90 100 110 120 SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SERDEDGFLY MVYASQETFG TALAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B." Mann S.S., Hammarback J.A. J. Biol. Chem. 269:11492-11497(1994) [PubMed: 7908909] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain.
[2]	"Cloning and characterization of rat lc3 orthologs." Dang Y., Yu L., Wu J., Pei Y. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: Sprague-Dawley. Tissue: Brain.
[3]	"Rattus norvegicus microtubule-associated protein 1 light chain 3 (Map1lc3)." Zhou G., Dai F., Yu L. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: Sprague-Dawley.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Heart and Pituitary.
[5]	Lubec G., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 31-37, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
[6]	"LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing." Kabeya Y., Mizushima N., Ueno T., Yamamoto A., Kirisako T., Noda T., Kominami E., Ohsumi Y., Yoshimori T. EMBO J. 19:5720-5728(2000) [PubMed: 11060023] [Abstract] Cited for: SUBCELLULAR LOCATION, CLEAVAGE, MUTAGENESIS OF GLY-120.
[7]	"LC3 conjugation system in mammalian autophagy." Tanida I., Ueno T., Kominami E. Int. J. Biochem. Cell Biol. 36:2503-2518(2004) [PubMed: 15325588] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U05784 mRNA. Translation: AAA20645.1.
AY206669 mRNA. Translation: AAP42561.1.
AY392036 mRNA. Translation: AAQ94605.1.
BC058144 mRNA. Translation: AAH58144.1.
BC083556 mRNA. Translation: AAH83556.1.

PIR

A53624.

RefSeq

NP_074058.2.

UniGene

Rn.41412

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UGM	X-ray	2.05	A	1-120	[»]
2Z0D	X-ray	1.90	B	1-120	[»]
2Z0E	X-ray	1.90	B	1-124	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSRNOG00000038106. Rattus norvegicus. [Contig view]

GeneID

64862.

KEGG

rno:64862.

Organism-specific databases

RGD

621315. Map1lc3b.

Phylogenomic databases

HOVERGEN

Q62625.

Gene expression databases

ArrayExpress

Q62625.

GermOnline

ENSRNOG00000038106. Rattus norvegicus.

Family and domain databases

InterPro

IPR004241. MAP1_LC3.
[Graphical view]

PANTHER

PTHR10969. MAP1_LC3. 1 hit.

Pfam

PF02991. MAP1_LC3. 1 hit.
[Graphical view]

ProDom

Q62625.
[Graphical view] [Entries sharing at least one domain]

BLOCKS

Search...

Other Resources

ProtoNet

Search...

Entry information

Entry name

MLP3B_RAT

Accession

Primary (citable) accession number: Q62625
Secondary accession number(s): Q6XVN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 23, 2007
Last modified:	July 22, 2008
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)