Reviewed,
UniProtKB/Swiss-Prot Q62625 (MLP3B_RAT)
Last modified
July 22, 2008.
Version 65.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Microtubule-associated proteins 1A/1B light chain 3B Alternative name(s): Microtubule-associated protein 1 light chain 3 beta MAP1A/1B light chain 3 B MAP1A/MAP1B LC3 B MAP1 light chain 3-like protein 2 Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 142 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Probably involved in formation of autophagosomal vacuoles (autophagosomes). |
| Subunit structure | 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. |
| Subcellular location | Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Note= LC3-II binds to the autophagic membranes. |
| Tissue specificity | Abundant only in neurons. Detected in testes. |
| Post-translational modification | The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II. |
| Sequence similarities | Belongs to the MAP1 LC3 family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Autophagy Ubl conjugation pathway |
| Cellular component | Cytoplasm Cytoplasmic vesicle Membrane Microtubule |
| Coding sequence diversity | Alternative splicing |
| PTM | Lipoprotein |
| Technical term | 3D-structure Direct protein sequencing |
Gene Ontology (GO) | |
| Cellular component | autophagic vacuole membrane Inferred from direct assay. Source: UniProtKB |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | |||||
| Isoform 1 (identifier: Q62625-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | |||||
| Isoform 2 (identifier: Q62625-2) The sequence of this isoform differs from the canonical sequence as follows: 126-142: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | |||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||||||||||||||||||
| Chain | 2 – 120 | 119 | Microtubule-associated proteins 1A/1B light chain 3B | ||||||||||||||||||||||
| Propeptide | 121 – 142 | 22 | Removed in mature form Probable | ||||||||||||||||||||||
Amino acid modifications | |||||||||||||||||||||||||
| Lipidation | 120 | 1 | Phosphatidylethanolamine amidated glycine By similarity | ||||||||||||||||||||||
Natural variations | |||||||||||||||||||||||||
| Alternative sequence | 126 – 142 | 17 | Missing in isoform 2. | ||||||||||||||||||||||
Experimental info | |||||||||||||||||||||||||
| Mutagenesis | 120 | 1 | G → A: No processing of precursor | ||||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||
| Helix | 7 – 10 | 4 | |||||||||||||||||||||||
| Helix | 13 – 26 | 14 | |||||||||||||||||||||||
| Beta strand | 30 – 37 | 8 | |||||||||||||||||||||||
| Beta strand | 50 – 55 | 6 | |||||||||||||||||||||||
| Helix | 60 – 70 | 11 | |||||||||||||||||||||||
| Beta strand | 80 – 83 | 4 | |||||||||||||||||||||||
| Beta strand | 91 – 94 | 4 | |||||||||||||||||||||||
| Helix | 95 – 102 | 8 | |||||||||||||||||||||||
| Beta strand | 109 – 115 | 7 | |||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B." Mann S.S., Hammarback J.A. J. Biol. Chem. 269:11492-11497(1994) [PubMed: 7908909] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain. |
| [2] | "Cloning and characterization of rat lc3 orthologs." Dang Y., Yu L., Wu J., Pei Y. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: Sprague-Dawley. Tissue: Brain. |
| [3] | "Rattus norvegicus microtubule-associated protein 1 light chain 3 (Map1lc3)." Zhou G., Dai F., Yu L. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: Sprague-Dawley. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Heart and Pituitary. |
| [5] | Lubec G., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 31-37, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain. |
| [6] | "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing." Kabeya Y., Mizushima N., Ueno T., Yamamoto A., Kirisako T., Noda T., Kominami E., Ohsumi Y., Yoshimori T. EMBO J. 19:5720-5728(2000) [PubMed: 11060023] [Abstract] Cited for: SUBCELLULAR LOCATION, CLEAVAGE, MUTAGENESIS OF GLY-120. |
| [7] | "LC3 conjugation system in mammalian autophagy." Tanida I., Ueno T., Kominami E. Int. J. Biochem. Cell Biol. 36:2503-2518(2004) [PubMed: 15325588] [Abstract] Cited for: REVIEW. |
Cross-references
Sequence databases | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| U05784 mRNA. Translation: AAA20645.1. AY206669 mRNA. Translation: AAP42561.1. AY392036 mRNA. Translation: AAQ94605.1. BC058144 mRNA. Translation: AAH58144.1. BC083556 mRNA. Translation: AAH83556.1. | |||||||||||||||||||||||||
| PIR | A53624. | ||||||||||||||||||||||||
| RefSeq | NP_074058.2. | ||||||||||||||||||||||||
| UniGene | Rn.41412 | ||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||
| Ensembl | ENSRNOG00000038106. Rattus norvegicus. [Contig view] | ||||||||||||||||||||||||
| GeneID | 64862. | ||||||||||||||||||||||||
| KEGG | rno:64862. | ||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||
| RGD | 621315. Map1lc3b. | ||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||
| HOVERGEN | Q62625. | ||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||
| ArrayExpress | Q62625. | ||||||||||||||||||||||||
| GermOnline | ENSRNOG00000038106. Rattus norvegicus. | ||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||
| InterPro | IPR004241. MAP1_LC3. [Graphical view] | ||||||||||||||||||||||||
| PANTHER | PTHR10969. MAP1_LC3. 1 hit. | ||||||||||||||||||||||||
| Pfam | PF02991. MAP1_LC3. 1 hit. [Graphical view] | ||||||||||||||||||||||||
| ProDom | Q62625. [Graphical view] [Entries sharing at least one domain] | ||||||||||||||||||||||||
| BLOCKS | Search... | ||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||
Entry information
| Entry name | MLP3B_RAT | ||||||||
| Accession | Primary (citable) accession number: Q62625 Secondary accession number(s): Q6XVN7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

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