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Protein

Cell division cycle protein 20 homolog

Gene

Cdc20

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation (By similarity). Interacts with NEK2 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-141405 Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components
R-RNO-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-RNO-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-RNO-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-RNO-174113 SCF-beta-TrCP mediated degradation of Emi1
R-RNO-174154 APC/C:Cdc20 mediated degradation of Securin
R-RNO-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-RNO-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-RNO-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-RNO-176408 Regulation of APC/C activators between G1/S and early anaphase
R-RNO-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-RNO-176417 Phosphorylation of Emi1
R-RNO-179409 APC-Cdc20 mediated degradation of Nek2A
R-RNO-2467813 Separation of Sister Chromatids
R-RNO-2500257 Resolution of Sister Chromatid Cohesion
R-RNO-5663220 RHO GTPases Activate Formins
R-RNO-5689880 Ub-specific processing proteases
R-RNO-68877 Mitotic Prometaphase
R-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein 20 homolog
Alternative name(s):
p55CDC
Gene namesi
Name:Cdc20
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620477 Cdc20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509021 – 499Cell division cycle protein 20 homologAdd BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei41PhosphoserineBy similarity1
Modified residuei66N6-acetyllysineBy similarity1
Modified residuei70PhosphothreonineBy similarity1
Modified residuei72PhosphoserineBy similarity1
Modified residuei92PhosphoserineBy similarity1
Modified residuei106PhosphothreonineBy similarity1
Modified residuei153PhosphoserineBy similarity1
Modified residuei157PhosphothreonineBy similarity1
Modified residuei161PhosphoserineBy similarity1
Cross-linki485Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki490Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2 (By similarity).By similarity
Dephosphorylated by CTDP1.By similarity
Acetylated. Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C) (By similarity).By similarity
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62623
PRIDEiQ62623

PTM databases

iPTMnetiQ62623
PhosphoSitePlusiQ62623

Expressioni

Gene expression databases

BgeeiENSRNOG00000028415
GenevisibleiQ62623 RN

Interactioni

Subunit structurei

Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with MAD2L1, BUB1B, SIRT2 and SPATC1. The phosphorylated form interacts with APC/C. Interacts with NINL and MAD2L1. Interacts with CDK5RAP2. Interacts with NEUROD2. Interacts with HSF1 (via phosphorylated form); this interaction occurs in mitosis in a MAD2L1-dependent manner and prevents PLK1-stimulated degradation of HSF1 by blocking the recruitment of the SCF(BTRC) ubiquitin ligase complex.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
HDAC6Q9UBN72EBI-2256532,EBI-301697From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249100, 6 interactors
IntActiQ62623, 3 interactors
STRINGi10116.ENSRNOP00000037772

Structurei

3D structure databases

ProteinModelPortaliQ62623
SMRiQ62623
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati182 – 221WD 1Add BLAST40
Repeati224 – 263WD 2Add BLAST40
Repeati266 – 303WD 3Add BLAST38
Repeati307 – 346WD 4Add BLAST40
Repeati353 – 395WD 5Add BLAST43
Repeati397 – 438WD 6Add BLAST42
Repeati441 – 480WD 7Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0305 Eukaryota
ENOG410XQ8I LUCA
GeneTreeiENSGT00870000136444
HOGENOMiHOG000195514
HOVERGENiHBG001024
InParanoidiQ62623
KOiK03363
OMAiWQSNILA
OrthoDBiEOG091G06FJ
PhylomeDBiQ62623

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR024977 Apc4_WD40_dom
IPR033187 CDC20
IPR033010 Cdc20/Fizzy
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PANTHERiPTHR19918 PTHR19918, 1 hit
PTHR19918:SF3 PTHR19918:SF3, 1 hit
PfamiView protein in Pfam
PF12894 ANAPC4_WD40, 1 hit
PF00400 WD40, 3 hits
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 3 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q62623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQFVFESDL HSLLQLDAPI PNAPIARWQR KAKEATGPAP SPMRAANRSH
60 70 80 90 100
SAGRTPGRTP GKSNSKVQTT PSKPGGDRYI PQRSASQMEV ASFLLSKENQ
110 120 130 140 150
PEDGGTPTKK EHQKAWARNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV
160 170 180 190 200
LYSQKATPGS SRKACRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV
210 220 230 240 250
ALDNSVYLWN AGSGDILQLL QMEQPGDYIS SVAWIKEGNY LAVGTSNAEV
260 270 280 290 300
QLWDVQQQKR LRNMTSHSAR VSSLSWNSYI LSSGSRSGHI HHHDVRVAEH
310 320 330 340 350
HVATLSGHSQ EVCGLRWAPD GRHLASGGND NIVNVWPSGP GESGWVPLQT
360 370 380 390 400
FTQHQGAVKA VAWCPWQSNI LATGGGTSDR HIRIWNVCSG ACLSAVDVHS
410 420 430 440 450
QVCSILWSPH YKELISGHGF AQNQLVIWKY PTMAKVAELK GHTARVLSLT
460 470 480 490
MSPDGATVAS AAADETLRLW RCFELDPALR REREKASTSK SSLIHQGIR
Length:499
Mass (Da):54,829
Last modified:May 1, 2007 - v2
Checksum:i74B6261B57E40869
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77D → E in AAA19018 (PubMed:7513050).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05341 mRNA Translation: AAA19018.1
AF052695 Genomic DNA Translation: AAC14741.1
BC085691 mRNA Translation: AAH85691.1
PIRiB56021
RefSeqiNP_741990.1, NM_171993.1
UniGeneiRn.9262

Genome annotation databases

EnsembliENSRNOT00000036151; ENSRNOP00000037772; ENSRNOG00000028415
GeneIDi64515
KEGGirno:64515
UCSCiRGD:620477 rat

Similar proteinsi

Entry informationi

Entry nameiCDC20_RAT
AccessioniPrimary (citable) accession number: Q62623
Secondary accession number(s): O70380, Q5U360
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: May 1, 2007
Last modified: May 23, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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