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Protein

Cell division cycle protein 20 homolog

Gene

Cdc20

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation (By similarity). Interacts with NEK2 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • histone deacetylase binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-141405. Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components.
R-RNO-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-RNO-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-RNO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-RNO-174154. APC/C:Cdc20 mediated degradation of Securin.
R-RNO-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-RNO-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-RNO-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-RNO-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-RNO-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-RNO-176417. Phosphorylation of Emi1.
R-RNO-179409. APC-Cdc20 mediated degradation of Nek2A.
R-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.
R-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein 20 homolog
Alternative name(s):
p55CDC
Gene namesi
Name:Cdc20
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620477. Cdc20.

Subcellular locationi

GO - Cellular componenti

  • anaphase-promoting complex Source: Ensembl
  • centrosome Source: RGD
  • nucleoplasm Source: Ensembl
  • perinuclear region of cytoplasm Source: RGD
  • protein complex Source: RGD
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Cell division cycle protein 20 homologPRO_0000050902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei41 – 411PhosphoserineBy similarity
Modified residuei66 – 661N6-acetyllysineBy similarity
Modified residuei70 – 701PhosphothreonineBy similarity
Modified residuei72 – 721PhosphoserineBy similarity
Modified residuei92 – 921PhosphoserineBy similarity
Modified residuei106 – 1061PhosphothreonineBy similarity
Modified residuei153 – 1531PhosphoserineBy similarity
Modified residuei157 – 1571PhosphothreonineBy similarity
Modified residuei161 – 1611PhosphoserineBy similarity
Cross-linki485 – 485Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki490 – 490Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2 (By similarity).By similarity
Dephosphorylated by CTDP1.By similarity
Acetylated. Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C) (By similarity).By similarity
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ62623.
PRIDEiQ62623.

PTM databases

iPTMnetiQ62623.
PhosphoSiteiQ62623.

Expressioni

Gene expression databases

GenevisibleiQ62623. RN.

Interactioni

Subunit structurei

Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with MAD2L1, BUB1B, SIRT2 and SPATC1. The phosphorylated form interacts with APC/C. Interacts with NINL and MAD2L1 (By similarity). Interacts with CDK5RAP2. Interacts with NEUROD2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
HDAC6Q9UBN72EBI-2256532,EBI-301697From a different organism.

GO - Molecular functioni

  • histone deacetylase binding Source: RGD

Protein-protein interaction databases

BioGridi249100. 6 interactions.
IntActiQ62623. 3 interactions.
STRINGi10116.ENSRNOP00000037772.

Structurei

3D structure databases

ProteinModelPortaliQ62623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati182 – 22140WD 1Add
BLAST
Repeati224 – 26340WD 2Add
BLAST
Repeati266 – 30338WD 3Add
BLAST
Repeati307 – 34640WD 4Add
BLAST
Repeati353 – 39543WD 5Add
BLAST
Repeati397 – 43842WD 6Add
BLAST
Repeati441 – 48040WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0305. Eukaryota.
ENOG410XQ8I. LUCA.
GeneTreeiENSGT00840000129804.
HOGENOMiHOG000195514.
HOVERGENiHBG001024.
InParanoidiQ62623.
KOiK03363.
OMAiHRNASQM.
OrthoDBiEOG76X602.
PhylomeDBiQ62623.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQFVFESDL HSLLQLDAPI PNAPIARWQR KAKEATGPAP SPMRAANRSH
60 70 80 90 100
SAGRTPGRTP GKSNSKVQTT PSKPGGDRYI PQRSASQMEV ASFLLSKENQ
110 120 130 140 150
PEDGGTPTKK EHQKAWARNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV
160 170 180 190 200
LYSQKATPGS SRKACRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV
210 220 230 240 250
ALDNSVYLWN AGSGDILQLL QMEQPGDYIS SVAWIKEGNY LAVGTSNAEV
260 270 280 290 300
QLWDVQQQKR LRNMTSHSAR VSSLSWNSYI LSSGSRSGHI HHHDVRVAEH
310 320 330 340 350
HVATLSGHSQ EVCGLRWAPD GRHLASGGND NIVNVWPSGP GESGWVPLQT
360 370 380 390 400
FTQHQGAVKA VAWCPWQSNI LATGGGTSDR HIRIWNVCSG ACLSAVDVHS
410 420 430 440 450
QVCSILWSPH YKELISGHGF AQNQLVIWKY PTMAKVAELK GHTARVLSLT
460 470 480 490
MSPDGATVAS AAADETLRLW RCFELDPALR REREKASTSK SSLIHQGIR
Length:499
Mass (Da):54,829
Last modified:May 1, 2007 - v2
Checksum:i74B6261B57E40869
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771D → E in AAA19018 (PubMed:7513050).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05341 mRNA. Translation: AAA19018.1.
AF052695 Genomic DNA. Translation: AAC14741.1.
BC085691 mRNA. Translation: AAH85691.1.
PIRiB56021.
RefSeqiNP_741990.1. NM_171993.1.
UniGeneiRn.9262.

Genome annotation databases

EnsembliENSRNOT00000036151; ENSRNOP00000037772; ENSRNOG00000028415.
GeneIDi64515.
KEGGirno:64515.
UCSCiRGD:620477. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05341 mRNA. Translation: AAA19018.1.
AF052695 Genomic DNA. Translation: AAC14741.1.
BC085691 mRNA. Translation: AAH85691.1.
PIRiB56021.
RefSeqiNP_741990.1. NM_171993.1.
UniGeneiRn.9262.

3D structure databases

ProteinModelPortaliQ62623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249100. 6 interactions.
IntActiQ62623. 3 interactions.
STRINGi10116.ENSRNOP00000037772.

PTM databases

iPTMnetiQ62623.
PhosphoSiteiQ62623.

Proteomic databases

PaxDbiQ62623.
PRIDEiQ62623.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000036151; ENSRNOP00000037772; ENSRNOG00000028415.
GeneIDi64515.
KEGGirno:64515.
UCSCiRGD:620477. rat.

Organism-specific databases

CTDi991.
RGDi620477. Cdc20.

Phylogenomic databases

eggNOGiKOG0305. Eukaryota.
ENOG410XQ8I. LUCA.
GeneTreeiENSGT00840000129804.
HOGENOMiHOG000195514.
HOVERGENiHBG001024.
InParanoidiQ62623.
KOiK03363.
OMAiHRNASQM.
OrthoDBiEOG76X602.
PhylomeDBiQ62623.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-RNO-141405. Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components.
R-RNO-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-RNO-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-RNO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-RNO-174154. APC/C:Cdc20 mediated degradation of Securin.
R-RNO-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-RNO-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-RNO-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-RNO-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-RNO-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-RNO-176417. Phosphorylation of Emi1.
R-RNO-179409. APC-Cdc20 mediated degradation of Nek2A.
R-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-5663220. RHO GTPases Activate Formins.
R-RNO-68877. Mitotic Prometaphase.
R-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi613310.
PROiQ62623.

Gene expression databases

GenevisibleiQ62623. RN.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024977. Apc4_WD40_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel mammalian protein, p55CDC, present in dividing cells is associated with protein kinase activity and has homology to the Saccharomyces cerevisiae cell division cycle proteins Cdc20 and Cdc4."
    Weinstein J., Jacobsen F.W., Hsu-Chen J., Wu T., Baum L.G.
    Mol. Cell. Biol. 14:3350-3363(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "Genomic organization, 5' flanking enhancer region, and chromosomal assignment of the cell cycle gene, p55Cdc."
    Weinstein J., Karim J., Geschwind D.H., Nelson S.F., Krumm J., Sakamoto K.M.
    Mol. Genet. Metab. 64:52-57(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiCDC20_RAT
AccessioniPrimary (citable) accession number: Q62623
Secondary accession number(s): O70380, Q5U360
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: May 1, 2007
Last modified: May 11, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.