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Q62622

- 4EBP1_RAT

UniProt

Q62622 - 4EBP1_RAT

Protein

Eukaryotic translation initiation factor 4E-binding protein 1

Gene

Eif4ebp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.1 Publication

    GO - Molecular functioni

    1. eukaryotic initiation factor 4E binding Source: RGD
    2. translation initiation factor binding Source: RGD
    3. translation repressor activity Source: UniProtKB

    GO - Biological processi

    1. G1/S transition of mitotic cell cycle Source: Ensembl
    2. insulin receptor signaling pathway Source: Ensembl
    3. lung development Source: RGD
    4. negative regulation of protein complex assembly Source: RGD
    5. negative regulation of translational initiation Source: RGD
    6. positive regulation of mitotic cell cycle Source: Ensembl
    7. response to ethanol Source: RGD
    8. response to ischemia Source: RGD
    9. TOR signaling Source: UniProtKB

    Keywords - Molecular functioni

    Protein synthesis inhibitor

    Keywords - Biological processi

    Translation regulation

    Enzyme and pathway databases

    ReactomeiREACT_219577. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 4E-binding protein 1
    Short name:
    4E-BP1
    Short name:
    eIF4E-binding protein 1
    Alternative name(s):
    Phosphorylated heat- and acid-stable protein regulated by insulin 1
    Short name:
    PHAS-I
    Gene namesi
    Name:Eif4ebp1
    OrganismiRattus norvegicus (Rat)Imported
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 16

    Organism-specific databases

    RGDi620259. Eif4ebp1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. nucleus Source: RGD
    3. protein complex Source: RGD

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi64 – 641S → A: Decreases phosphorylation by MAPK1 and MAPK3. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 117116Eukaryotic translation initiation factor 4E-binding protein 1PRO_0000190515Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei36 – 361Phosphothreonine; by MTORBy similarity
    Modified residuei40 – 401PhosphothreonineBy similarity
    Modified residuei45 – 451Phosphothreonine; by MTORBy similarity
    Modified residuei49 – 491PhosphothreonineBy similarity
    Modified residuei53 – 531PhosphotyrosineBy similarity
    Cross-linki56 – 56Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei64 – 641Phosphoserine; by DYRK2, MAPK1, MAPK3 and MTOR2 Publications
    Modified residuei69 – 691Phosphothreonine; by MTORBy similarity
    Modified residuei82 – 821PhosphoserineBy similarity
    Modified residuei100 – 1001Phosphoserine; by DYRK2By similarity
    Modified residuei111 – 1111PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-36, Thr-45, Ser-64 and Thr-69, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.2 Publications
    Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-36, Thr-45, Ser-64 and Thr-69) or associated with eIF4E By similarity.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ62622.

    PTM databases

    PhosphoSiteiQ62622.

    Miscellaneous databases

    PMAP-CutDBQ62622.

    Expressioni

    Tissue specificityi

    Expressed in all tissues examined; highest levels in fat and skeletal tissue, lowest levels in kidney.2 Publications

    Gene expression databases

    GenevestigatoriQ62622.

    Interactioni

    Subunit structurei

    Hypophosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts with RPTOR.1 Publication

    Protein-protein interaction databases

    BioGridi250521. 3 interactions.
    DIPiDIP-267N.
    MINTiMINT-94776.
    STRINGi10116.ENSRNOP00000016885.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the eIF4E-binding protein family.Curated

    Phylogenomic databases

    eggNOGiNOG78084.
    GeneTreeiENSGT00390000013843.
    HOGENOMiHOG000231190.
    HOVERGENiHBG050425.
    InParanoidiQ62622.
    KOiK07205.
    OMAiNHLRNSP.
    OrthoDBiEOG7B31Q1.
    PhylomeDBiQ62622.
    TreeFamiTF101530.

    Family and domain databases

    InterProiIPR008606. EIF4EBP.
    [Graphical view]
    PfamiPF05456. eIF_4EBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q62622-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAGSSCSQT PSRAIPTRRV ALGDGVQLPP GDYSTTPGGT LFSTTPGGTR    50
    IIYDRKFLME CRNSPVAKTP PKDLPTIPGV TSPTSDEPPM QASQSHLHSS 100
    PEDKRAGGEE SQFEMDI 117
    Length:117
    Mass (Da):12,404
    Last modified:January 23, 2007 - v3
    Checksum:i3449D57B09FA101A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti19 – 191R → N AA sequence (PubMed:8170978)Curated
    Sequence conflicti69 – 691T → P AA sequence (PubMed:8170978)Curated
    Sequence conflicti75 – 751P → L AA sequence (PubMed:8170978)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05014 mRNA. Translation: AAA86938.1.
    PIRiA55258.
    RefSeqiNP_446309.1. NM_053857.2.
    UniGeneiRn.11161.

    Genome annotation databases

    EnsembliENSRNOT00000016885; ENSRNOP00000016885; ENSRNOG00000012582.
    GeneIDi116636.
    KEGGirno:116636.
    UCSCiRGD:620259. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05014 mRNA. Translation: AAA86938.1 .
    PIRi A55258.
    RefSeqi NP_446309.1. NM_053857.2.
    UniGenei Rn.11161.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250521. 3 interactions.
    DIPi DIP-267N.
    MINTi MINT-94776.
    STRINGi 10116.ENSRNOP00000016885.

    PTM databases

    PhosphoSitei Q62622.

    Proteomic databases

    PRIDEi Q62622.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000016885 ; ENSRNOP00000016885 ; ENSRNOG00000012582 .
    GeneIDi 116636.
    KEGGi rno:116636.
    UCSCi RGD:620259. rat.

    Organism-specific databases

    CTDi 1978.
    RGDi 620259. Eif4ebp1.

    Phylogenomic databases

    eggNOGi NOG78084.
    GeneTreei ENSGT00390000013843.
    HOGENOMi HOG000231190.
    HOVERGENi HBG050425.
    InParanoidi Q62622.
    KOi K07205.
    OMAi NHLRNSP.
    OrthoDBi EOG7B31Q1.
    PhylomeDBi Q62622.
    TreeFami TF101530.

    Enzyme and pathway databases

    Reactomei REACT_219577. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.

    Miscellaneous databases

    NextBioi 619343.
    PMAP-CutDB Q62622.
    PROi Q62622.

    Gene expression databases

    Genevestigatori Q62622.

    Family and domain databases

    InterProi IPR008606. EIF4EBP.
    [Graphical view ]
    Pfami PF05456. eIF_4EBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and tissue distribution of PHAS-I, an intracellular target for insulin and growth factors."
      Hu C., Pang S., Kong X., Velleca M., Lawrence J.C. Jr.
      Proc. Natl. Acad. Sci. U.S.A. 91:3730-3734(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-33; 43-53; 62-80 AND 98-117, PHOSPHORYLATION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley1 Publication.
      Tissue: Adipocyte1 Publication and Skeletal muscleImported.
    2. "PHAS-I as a link between mitogen-activated protein kinase and translation initiation."
      Lin T.-A., Kong X., Haystead T.A.J., Pause A., Belsham G.J., Sonenberg N., Lawrence J.C. Jr.
      Science 266:653-656(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH EIF4E, PHOSPHORYLATION AT SER-64 BY MAPK1 AND MAPK3, MUTAGENESIS OF SER-64.

    Entry informationi

    Entry namei4EBP1_RAT
    AccessioniPrimary (citable) accession number: Q62622
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 95 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3