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Q62521 (ZIC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc finger protein ZIC 3
Alternative name(s):
Zinc finger protein of the cerebellum 3
Gene names
Name:Zic3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. Ref.5 Ref.7 Ref.8

Subunit structure

Interacts with KPNA1 and KPNA6. Interacts (via C2H2-type domains 3, 4 and 5) with GLI3; the interaction enhances its transcriptional activity By similarity. Interacts (via the C2H2-type domains 3, 4 and 5) with MDFIC (via the C2H2-type domains 3, 4 and 5); the interaction reduces its transcriptional activity. Ref.8

Subcellular location

Nucleus. Cytoplasm. Note: Translocation to the nucleus requires KPNA1 or KPNA6 By similarity. Localizes in the cytoplasm in presence of MDFIC overexpression. Ref.6

Tissue specificity

CNS. A high level expression is seen in the cerebellum.

Developmental stage

Expressed in the CNS, tailbud and somites. Ref.7

Domain

The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.

Disruption phenotype

Some mice exhibit embryonic and postnatal lethality. Viable mice show heart disease, disturbances of laterality, neural tube defects and vertebral and rib defects. Ref.7

Sequence similarities

Belongs to the GLI C2H2-type zinc-finger protein family.

Contains 5 C2H2-type zinc fingers.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
Developmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterior/posterior pattern specification

Inferred from genetic interaction PubMed 16496285. Source: MGI

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

determination of digestive tract left/right asymmetry

Inferred from electronic annotation. Source: Ensembl

determination of left/right asymmetry in nervous system

Inferred from mutant phenotype Ref.7. Source: UniProtKB

determination of left/right symmetry

Inferred from mutant phenotype PubMed 15384171PubMed 16496285. Source: MGI

determination of liver left/right asymmetry

Inferred from electronic annotation. Source: Ensembl

determination of pancreatic left/right asymmetry

Inferred from electronic annotation. Source: Ensembl

heart looping

Inferred from mutant phenotype PubMed 15384171PubMed 16496285. Source: MGI

lung development

Inferred from electronic annotation. Source: Ensembl

pattern specification process

Inferred from mutant phenotype Ref.7. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16496285. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay Ref.5. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from direct assay Ref.8. Source: UniProtKB

nucleus

Inferred from direct assay Ref.6Ref.8. Source: UniProtKB

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction Ref.8. Source: UniProtKB

sequence-specific DNA binding

Inferred from sequence or structural similarity. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.5. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q62521-1)

Also known as: Zic3-A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q62521-2)

Also known as: Zic3-B;

The sequence of this isoform differs from the canonical sequence as follows:
     408-466: VHESQGSDSS...LPPNFNEWYV → CCPAWYLGQS...AEPTVQEMIY

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Zinc finger protein ZIC 3
PRO_0000047251

Regions

Zinc finger250 – 28536C2H2-type 1; atypical
Zinc finger294 – 32128C2H2-type 2; atypical
Zinc finger327 – 35125C2H2-type 3
Zinc finger357 – 38125C2H2-type 4
Zinc finger387 – 40923C2H2-type 5
Region296 – 32126Nuclear localization signal By similarity
Region329 – 35123Nuclear localization signal By similarity
Compositional bias46 – 5510Poly-Ala
Compositional bias87 – 9610Poly-His

Natural variations

Alternative sequence408 – 46659VHESQ…NEWYV → CCPAWYLGQSLIPDEELDTD VGMQQPVLHNTSYPKCRVNA EPTVQEMIY in isoform 2.
VSP_044011

Experimental info

Sequence conflict104 – 1063GGA → ARR in BAA11116. Ref.1
Isoform 2:
Sequence conflict4191I → V in BAC28831. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Zic3-A) [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: C92C06EC06DF601A

FASTA46650,446
        10         20         30         40         50         60 
MTMLLDGGPQ FPGLGVGSFG APRHHEMPNR EPAGMGLNPF GDSTHAAAAA AAAAAFKLSP 

        70         80         90        100        110        120 
ATAHDLSSGQ SSAFTPQGSG YANALGHHHH HHHHHHASQV PTYGGAASAA FNSTRDFLFR 

       130        140        150        160        170        180 
QRGSGLSEAA SGGGQHGLFA GSASSLHAPA GIPEPPSYLL FPGLHEQGAG HPSPTGHVDN 

       190        200        210        220        230        240 
NQVHLGLRGE LFGRADPYRP VASPRTDPYA ASAQFPNYSP MNMNMGVNVA AHHGPGAFFR 

       250        260        270        280        290        300 
YMRQPIKQEL SCKWIEEAQL SRPKKSCDRT FSTMHELVTH VTMEHVGGPE QNNHVCYWEE 

       310        320        330        340        350        360 
CPREGKSFKA KYKLVNHIRV HTGEKPFPCP FPGCGKIFAR SENLKIHKRT HTGEKPFKCE 

       370        380        390        400        410        420 
FEGCDRRFAN SSDRKKHMHV HTSDKPYICK VCDKSYTHPS SLRKHMKVHE SQGSDSSPAA 

       430        440        450        460 
SSGYESSTPP AIASANSKDT TKTPSAVQTS TSHNPGLPPN FNEWYV 

« Hide

Isoform 2 (Zic3-B) [UniParc].

Checksum: BD0ECE4D3780FBAA
Show »

FASTA45649,952

References

« Hide 'large scale' references
[1]"The mouse zic gene family. Homologues of the Drosophila pair-rule gene odd-paired."
Aruga J., Nagai T., Tokuyama T., Hayashizaki Y., Okazaki Y., Chapman V.M., Mikoshiba K.
J. Biol. Chem. 271:1043-1047(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Cerebellum.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Embryo and Testis.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Molecular properties of Zic proteins as transcriptional regulators and their relationship to GLI proteins."
Mizugishi K., Aruga J., Nakata K., Mikoshiba K.
J. Biol. Chem. 276:2180-2188(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING.
[6]"Physical and functional interactions between Zic and Gli proteins."
Koyabu Y., Nakata K., Mizugishi K., Aruga J., Mikoshiba K.
J. Biol. Chem. 276:6889-6892(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"A complex syndrome of left-right axis, central nervous system and axial skeleton defects in Zic3 mutant mice."
Purandare S.M., Ware S.M., Kwan K.M., Gebbia M., Bassi M.T., Deng J.M., Vogel H., Behringer R.R., Belmont J.W., Casey B.
Development 129:2293-2302(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[8]"Myogenic repressor I-mfa interferes with the function of Zic family proteins."
Mizugishi K., Hatayama M., Tohmonda T., Ogawa M., Inoue T., Mikoshiba K., Aruga J.
Biochem. Biophys. Res. Commun. 320:233-240(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MDFIC.
[9]"Identification of a novel ZIC3 isoform and mutation screening in patients with heterotaxy and congenital heart disease."
Bedard J.E., Haaning A.M., Ware S.M.
PLoS ONE 6:E23755-E23755(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D70849 mRNA. Translation: BAA11116.1.
AK134355 mRNA. Translation: BAE22110.1.
AK034780 mRNA. Translation: BAC28831.1.
AL954389 Genomic DNA. Translation: CAM20753.1.
AL954389, AL671920 Genomic DNA. Translation: CAM20751.1.
AL671920, AL954389 Genomic DNA. Translation: CAM24569.1.
CH466583 Genomic DNA. Translation: EDL42177.1.
CH466583 Genomic DNA. Translation: EDL42178.1.
CCDSCCDS30155.1. [Q62521-1]
RefSeqNP_033601.2. NM_009575.2. [Q62521-1]
UniGeneMm.255890.

3D structure databases

ProteinModelPortalQ62521.
SMRQ62521. Positions 244-409.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204696. 1 interaction.
IntActQ62521. 2 interactions.
MINTMINT-190020.
STRING10090.ENSMUSP00000085999.

Proteomic databases

PRIDEQ62521.

Protocols and materials databases

DNASU22773.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000088627; ENSMUSP00000085999; ENSMUSG00000067860. [Q62521-1]
ENSMUST00000088629; ENSMUSP00000086001; ENSMUSG00000067860. [Q62521-2]
GeneID22773.
KEGGmmu:22773.
UCSCuc009tho.1. mouse. [Q62521-1]
uc009thp.1. mouse.

Organism-specific databases

CTD7547.
MGIMGI:106676. Zic3.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00650000092933.
HOGENOMHOG000232057.
HOVERGENHBG007135.
InParanoidQ3UYV1.
KOK09224.
OMAKKTCDRT.
TreeFamTF351425.

Gene expression databases

ArrayExpressQ62521.
BgeeQ62521.
CleanExMM_ZIC3.
GenevestigatorQ62521.

Family and domain databases

Gene3D3.30.160.60. 4 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00096. zf-C2H2. 1 hit.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZIC3. mouse.
NextBio303317.
PROQ62521.
SOURCESearch...

Entry information

Entry nameZIC3_MOUSE
AccessionPrimary (citable) accession number: Q62521
Secondary accession number(s): A2AWK3, Q3UYV1, Q8BSB3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot