Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Eukaryotic translation initiation factor 4 gamma 2

Gene

Eif4g2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • regulation of translational initiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor, Repressor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4 gamma 2
Short name:
eIF-4-gamma 2
Short name:
eIF-4G 2
Short name:
eIF4G 2
Alternative name(s):
Novel APOBEC-1 target 1
Translation repressor NAT1
p97
Gene namesi
Name:Eif4g2Imported
Synonyms:Nat1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:109207. Eif4g2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • eukaryotic translation initiation factor 4F complex Source: UniProtKB
  • membrane Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 906906Eukaryotic translation initiation factor 4 gamma 2PRO_0000213326Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei394 – 3941PhosphoserineBy similarity
Modified residuei507 – 5071PhosphothreonineCombined sources
Modified residuei513 – 5131PhosphothreonineBy similarity
Modified residuei901 – 9011PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation; hyperphosphorylated during mitosis.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ62448.
MaxQBiQ62448.
PaxDbiQ62448.
PRIDEiQ62448.

PTM databases

iPTMnetiQ62448.
PhosphoSiteiQ62448.
SwissPalmiQ62448.

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues examined.1 Publication

Gene expression databases

BgeeiQ62448.

Interactioni

Subunit structurei

Interacts with the serine/threonine protein kinases MKNK1 and MKNK2. Binds EIF4A and EIF3. Interacts with MIF4GD (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Pdcd4Q618232EBI-296494,EBI-296473
Pik3r1P264503EBI-296494,EBI-641764

Protein-protein interaction databases

BioGridi199423. 1 interaction.
IntActiQ62448. 4 interactions.
MINTiMINT-1847505.
STRINGi10090.ENSMUSP00000124551.

Structurei

3D structure databases

ProteinModelPortaliQ62448.
SMRiQ62448. Positions 78-311, 539-896.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini78 – 308231MIF4GPROSITE-ProRule annotationAdd
BLAST
Domaini542 – 665124MIPROSITE-ProRule annotationAdd
BLAST
Domaini719 – 903185W2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MI domain.PROSITE-ProRule annotation
Contains 1 MIF4G domain.Curated
Contains 1 W2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IPIB. Eukaryota.
ENOG410ZIZB. LUCA.
HOVERGENiHBG052084.
InParanoidiQ62448.
KOiK03260.
PhylomeDBiQ62448.

Family and domain databases

Gene3Di1.25.40.180. 3 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
IPR003307. W2_domain.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
PF02854. MIF4G. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
SM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS51366. MI. 1 hit.
PS51363. W2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62448-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESAIAEGGA SRFSASSGGG GSRGAPQHYP KTAGNSEFLG KTPGQNAQKW
60 70 80 90 100
IPARSTRRDD NSAANNSANE KERHDAIFRK VRGILNKLTP EKFDKLCLEL
110 120 130 140 150
LNVGVESKLI LKGVILLIVD KALEEPKYSS LYAQLCLRLA EDAPNFDGPA
160 170 180 190 200
AEGQPGQKQS TTFRRLLISK LQDEFENRTR NVDVYDKREN PLLPEEEEQR
210 220 230 240 250
AIAKIKMLGN IKFIGELGKL DLIHESILHK CIKTLLEKKK RVQLKDMGED
260 270 280 290 300
LECLCQIMRT VGPRLDHERA KSLMDQYFAR MCSLMLSKEL PARIRFLLQD
310 320 330 340 350
TVELREHHWV PRKAFLDNGP KTINQIRQDA VKDLGVFIPA PMAQGRSDFF
360 370 380 390 400
LEGPFMPPRM KMDRDPLGGL ADMFGQMPGS GIGTGPGVIQ DRFSPTMGRH
410 420 430 440 450
RSNQLFNGHG GHIMPPTQSQ FGEMGGKFMK SQGLSQLYHN QSQGLLSQLQ
460 470 480 490 500
GQSKDMPPRF SKKGQLNADE ISLRPAQSFL MNKNQVPKLQ PQITMIPPSA
510 520 530 540 550
QPPRTQTPPL GQTPQLGLKT NPPLIQEKPA KTSKKPPPSK EELLKLTEAV
560 570 580 590 600
VTDYLNSGNA NDAVSGVREM RAPKHFLPEM LSKVIILSLD RSDEDKEKAS
610 620 630 640 650
SLISLLKQEG IATSDNFMQA FLNVLEQCPK LEVDIPLVKS YLAQFAARAI
660 670 680 690 700
ISELVSISEL AQPLESGTHF PLFLLCLQQL AKLQDREWLT ELFQQSKVNM
710 720 730 740 750
QKMLPEIDQN KDRMLEILEG KGLSFLFPLL KLEKELLKQI KLDPSPQTIY
760 770 780 790 800
KWIKDNISPK LHVDKGFVNI LMTSFLQYIS SEVSPPSDET DSSSAPSKEQ
810 820 830 840 850
LEQEKQLLLS FKPVMQKFLH DHVDLQVSAL YALQVHCYNS SFPKGMLLRF
860 870 880 890 900
FVHFYDMEII EEEAFLAWKE DITQEFPGKG KALFQVNQWL TWLETAEEEE

SEEEAD
Length:906
Mass (Da):102,106
Last modified:April 30, 2003 - v2
Checksum:iEA73E717671562E4
GO
Isoform 2 (identifier: Q62448-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     433-470: Missing.

Note: No experimental confirmation available.
Show »
Length:868
Mass (Da):97,894
Checksum:i4ACA2B2A42E2F3C3
GO

Sequence cautioni

The sequence BAE25826.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → V in BAE25826 (PubMed:16141072).Curated
Sequence conflicti14 – 141S → T in AAC53030 (PubMed:9027506).Curated
Sequence conflicti236 – 2361L → G in AAC53030 (PubMed:9027506).Curated
Sequence conflicti245 – 2451K → Q in AAC53095 (PubMed:9030685).Curated
Sequence conflicti346 – 3472RS → MSR in AAC53030 (PubMed:9027506).Curated
Sequence conflicti347 – 3471S → N in BAE25826 (PubMed:16141072).Curated
Sequence conflicti347 – 3471S → N in AAH40391 (PubMed:15489334).Curated
Sequence conflicti347 – 3471S → N in AAH43034 (PubMed:15489334).Curated
Sequence conflicti347 – 3471S → N in AAH56387 (PubMed:15489334).Curated
Sequence conflicti347 – 3471S → N in AAH57673 (PubMed:15489334).Curated
Sequence conflicti347 – 3471S → N in AAH64810 (PubMed:15489334).Curated
Sequence conflicti347 – 3471S → N in AAH92521 (PubMed:15489334).Curated
Sequence conflicti355 – 3551F → L in AAC53030 (PubMed:9027506).Curated
Sequence conflicti394 – 3941S → P in AAC53030 (PubMed:9027506).Curated
Sequence conflicti437 – 4371L → F in AAC53030 (PubMed:9027506).Curated
Sequence conflicti472 – 4721S → R in AAC53030 (PubMed:9027506).Curated
Sequence conflicti476 – 4761A → D in AAC53030 (PubMed:9027506).Curated
Sequence conflicti488 – 4881K → M in AAC53030 (PubMed:9027506).Curated
Sequence conflicti550 – 5501V → G in AAC53030 (PubMed:9027506).Curated
Sequence conflicti708 – 7081D → N in AAC53030 (PubMed:9027506).Curated
Sequence conflicti753 – 7531I → S in AAC53030 (PubMed:9027506).Curated
Sequence conflicti760 – 7601K → T in AAC53030 (PubMed:9027506).Curated
Sequence conflicti784 – 7841S → N in AAC53030 (PubMed:9027506).Curated
Sequence conflicti797 – 7982SK → PT in AAC53030 (PubMed:9027506).Curated
Sequence conflicti825 – 8251L → V in AAC53030 (PubMed:9027506).Curated
Sequence conflicti833 – 8331L → F in AAC53095 (PubMed:9030685).Curated
Sequence conflicti844 – 8441K → Q in AAC53030 (PubMed:9027506).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei433 – 47038Missing in isoform 2. 1 PublicationVSP_021640Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76112 mRNA. Translation: AAC53095.1.
U63323 mRNA. Translation: AAC53030.1.
AK144309 mRNA. Translation: BAE25826.1. Different initiation.
BC040391 mRNA. Translation: AAH40391.2.
BC043034 mRNA. Translation: AAH43034.2.
BC056387 mRNA. Translation: AAH56387.1.
BC057673 mRNA. Translation: AAH57673.2.
BC064810 mRNA. Translation: AAH64810.2.
BC092521 mRNA. Translation: AAH92521.1.
CCDSiCCDS40087.1. [Q62448-2]
CCDS40088.1. [Q62448-1]
RefSeqiNP_001035221.1. NM_001040131.2.
NP_038535.2. NM_013507.3.
UniGeneiMm.185453.

Genome annotation databases

GeneIDi13690.
KEGGimmu:13690.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76112 mRNA. Translation: AAC53095.1.
U63323 mRNA. Translation: AAC53030.1.
AK144309 mRNA. Translation: BAE25826.1. Different initiation.
BC040391 mRNA. Translation: AAH40391.2.
BC043034 mRNA. Translation: AAH43034.2.
BC056387 mRNA. Translation: AAH56387.1.
BC057673 mRNA. Translation: AAH57673.2.
BC064810 mRNA. Translation: AAH64810.2.
BC092521 mRNA. Translation: AAH92521.1.
CCDSiCCDS40087.1. [Q62448-2]
CCDS40088.1. [Q62448-1]
RefSeqiNP_001035221.1. NM_001040131.2.
NP_038535.2. NM_013507.3.
UniGeneiMm.185453.

3D structure databases

ProteinModelPortaliQ62448.
SMRiQ62448. Positions 78-311, 539-896.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199423. 1 interaction.
IntActiQ62448. 4 interactions.
MINTiMINT-1847505.
STRINGi10090.ENSMUSP00000124551.

PTM databases

iPTMnetiQ62448.
PhosphoSiteiQ62448.
SwissPalmiQ62448.

Proteomic databases

EPDiQ62448.
MaxQBiQ62448.
PaxDbiQ62448.
PRIDEiQ62448.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13690.
KEGGimmu:13690.

Organism-specific databases

CTDi1982.
MGIiMGI:109207. Eif4g2.

Phylogenomic databases

eggNOGiENOG410IPIB. Eukaryota.
ENOG410ZIZB. LUCA.
HOVERGENiHBG052084.
InParanoidiQ62448.
KOiK03260.
PhylomeDBiQ62448.

Miscellaneous databases

ChiTaRSiEif4g2. mouse.
PROiQ62448.
SOURCEiSearch...

Gene expression databases

BgeeiQ62448.

Family and domain databases

Gene3Di1.25.40.180. 3 hits.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
IPR003307. W2_domain.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
PF02854. MIF4G. 1 hit.
PF02020. W2. 1 hit.
[Graphical view]
SMARTiSM00515. eIF5C. 1 hit.
SM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS51366. MI. 1 hit.
PS51363. W2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel translational repressor mRNA is edited extensively in livers containing tumors caused by the transgene expression of the apoB mRNA-editing enzyme."
    Yamanaka S., Poksay K.S., Arnold K.S., Innerarity T.L.
    Genes Dev. 11:321-333(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION.
  2. "cDNA cloning, expression analysis, and chromosomal localization of a gene with high homology to wheat eIF-(iso)4F and mammalian eIF-4G."
    Shaughnessy J.D. Jr., Jenkins N.A., Copeland N.G.
    Genomics 39:192-197(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Brain1 Publication.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryonic lung.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J1 Publication and Czech II.
    Tissue: Embryonic brain, Eye and Mammary gland1 Publication.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-507, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiIF4G2_MOUSE
AccessioniPrimary (citable) accession number: Q62448
Secondary accession number(s): P97867
, Q0VGZ3, Q3UNC1, Q5XKD8, Q6P1Z0, Q921U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: June 8, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This gene has been shown to be extensively edited in the liver of APOBEC1 transgenic animals. Its aberrant editing could contribute to the potent oncogenesis induced by overexpression of APOBEC1. The aberrant edited sequence, called NAT1, is likely to be a fundamental translational repressor.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.