ID   VAMP1_MOUSE             Reviewed;         118 AA.
AC   Q62442; Q6PFF3; Q810K6;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-NOV-2009, entry version 87.
DE   RecName: Full=Vesicle-associated membrane protein 1;
DE            Short=VAMP-1;
DE   AltName: Full=Synaptobrevin-1;
GN   Name=Vamp1; Synonyms=Syb1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA   Martin S., Tellam J.T., Livington C., Slot J.W., Gould G.W.,
RA   James D.E.;
RL   Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   STRAIN=BALB/c; TISSUE=Brain;
RA   Olken S.K., Doerre S., Corley R.B.;
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=C57BL/6J; TISSUE=Cerebellum, and Medulla oblongata;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   STRAIN=C57BL/6; TISSUE=Retina;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   PROTEIN SEQUENCE OF 34-49; 50-58 AND 62-85, AND MASS SPECTROMETRY.
RC   STRAIN=C57BL/6, and OF1; TISSUE=Brain, and Hippocampus;
RA   Lubec G., Kang S.U., Sunyer B., Chen W.-Q.;
RL   Submitted (JAN-2009) to UniProtKB.
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Brain;
RX   PubMed=15648052; DOI=10.1002/pmic.200401066;
RA   Vosseller K., Hansen K.C., Chalkley R.J., Trinidad J.C., Wells L.,
RA   Hart G.W., Burlingame A.L.;
RT   "Quantitative analysis of both protein expression and serine /
RT   threonine post-translational modifications through stable isotope
RT   labeling with dithiothreitol.";
RL   Proteomics 5:388-398(2005).
CC   -!- FUNCTION: Involved in the targeting and/or fusion of transport
CC       vesicles to their target membrane.
CC   -!- SUBUNIT: Interacts with VAPA and VAPB (By similarity).
CC   -!- SUBCELLULAR LOCATION: Isoform 1: Cytoplasmic vesicle, secretory
CC       vesicle, synaptic vesicle membrane; Single-pass type IV membrane
CC       protein (By similarity). Cell junction, synapse, synaptosome (By
CC       similarity).
CC   -!- SUBCELLULAR LOCATION: Isoform 2: Cytoplasmic vesicle membrane;
CC       Single-pass type IV membrane protein (By similarity). Cell
CC       junction, synapse, synaptosome (By similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q62442-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q62442-2; Sequence=VSP_029186;
CC   -!- SIMILARITY: Belongs to the synaptobrevin family.
CC   -!- SIMILARITY: Contains 1 v-SNARE coiled-coil homology domain.
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DR   EMBL; U61751; AAB03491.1; -; mRNA.
DR   EMBL; AF007167; AAB62930.1; -; mRNA.
DR   EMBL; AK018783; BAB31407.1; -; mRNA.
DR   EMBL; AK078156; BAC37151.1; -; mRNA.
DR   EMBL; BC049902; AAH49902.2; -; mRNA.
DR   EMBL; BC057587; AAH57587.1; -; mRNA.
DR   IPI; IPI00125990; -.
DR   IPI; IPI00761439; -.
DR   RefSeq; NP_001074026.1; -.
DR   RefSeq; NP_033522.1; -.
DR   UniGene; Mm.32321; -.
DR   HSSP; P19065; 1F83.
DR   SMR; Q62442; 27-94.
DR   STRING; Q62442; -.
DR   PhosphoSite; Q62442; -.
DR   PRIDE; Q62442; -.
DR   Ensembl; ENSMUST00000032487; ENSMUSP00000032487; ENSMUSG00000030337; Mus musculus.
DR   Ensembl; ENSMUST00000063588; ENSMUSP00000063466; ENSMUSG00000030337; Mus musculus.
DR   GeneID; 22317; -.
DR   KEGG; mmu:22317; -.
DR   UCSC; uc009dtx.1; mouse.
DR   UCSC; uc009dty.1; mouse.
DR   CTD; 22317; -.
DR   MGI; MGI:1313276; Vamp1.
DR   HOVERGEN; Q62442; -.
DR   NextBio; 302523; -.
DR   ArrayExpress; Q62442; -.
DR   Bgee; Q62442; -.
DR   CleanEx; MM_VAMP1; -.
DR   Genevestigator; Q62442; -.
DR   GermOnline; ENSMUSG00000030337; Mus musculus.
DR   GO; GO:0030054; C:cell junction; IEA:UniProtKB-KW.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; IDA:MGI.
DR   GO; GO:0045202; C:synapse; IEA:UniProtKB-KW.
DR   GO; GO:0019717; C:synaptosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR   InterPro; IPR001388; Synaptobrevin.
DR   InterPro; IPR016444; Synaptobrevin_met/fun.
DR   Pfam; PF00957; Synaptobrevin; 1.
DR   PIRSF; PIRSF005409; Synaptobrevin_euk; 1.
DR   PRINTS; PR00219; SYNAPTOBREVN.
DR   ProDom; PD001229; Synaptobrevin; 1.
DR   PROSITE; PS00417; SYNAPTOBREVIN; 1.
DR   PROSITE; PS50892; V_SNARE; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cell junction; Coiled coil; Cytoplasmic vesicle;
KW   Direct protein sequencing; Membrane; Phosphoprotein; Synapse;
KW   Synaptosome; Transmembrane.
FT   CHAIN         1    118       Vesicle-associated membrane protein 1.
FT                                /FTId=PRO_0000206720.
FT   TOPO_DOM      1     96       Cytoplasmic (Potential).
FT   TRANSMEM     97    116       Anchor for type IV membrane protein
FT                                (Potential).
FT   TOPO_DOM    117    118       Vesicular (Potential).
FT   DOMAIN       33     93       v-SNARE coiled-coil homology.
FT   MOD_RES      63     63       Phosphoserine.
FT   VAR_SEQ     114    118       IYFFT -> SKYR (in isoform 2).
FT                                /FTId=VSP_029186.
SQ   SEQUENCE   118 AA;  12890 MW;  31CB8701446A7FB0 CRC64;
     MSAPAQPPAE GTEGAAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIMRV NVDKVLERDQ
     KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM LGAICAIIVV VIVIYFFT
//