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Protein

Vesicle-associated membrane protein 1

Gene

Vamp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the targeting and/or fusion of transport vesicles to their target membrane.

GO - Molecular functioni

  1. SNAP receptor activity Source: GO_Central
  2. SNARE binding Source: GO_Central

GO - Biological processi

  1. exocytosis Source: GO_Central
  2. neurotransmitter secretion Source: GO_Central
  3. SNARE complex assembly Source: BHF-UCL
  4. vesicle fusion Source: GO_Central
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 1
Short name:
VAMP-1
Alternative name(s):
Synaptobrevin-1
Gene namesi
Name:Vamp1
Synonyms:Syb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1313276. Vamp1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9696CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei97 – 11620Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
BLAST
Topological domaini117 – 1182VesicularSequence Analysis

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cell surface Source: BHF-UCL
  3. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
  5. mitochondrion Source: MGI
  6. neuron projection Source: UniProtKB-SubCell
  7. SNARE complex Source: GO_Central
  8. synaptic vesicle Source: GO_Central
  9. synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 118118Vesicle-associated membrane protein 1PRO_0000206720Add
BLAST

Proteomic databases

MaxQBiQ62442.
PaxDbiQ62442.
PRIDEiQ62442.

PTM databases

PhosphoSiteiQ62442.

Expressioni

Gene expression databases

BgeeiQ62442.
CleanExiMM_VAMP1.
ExpressionAtlasiQ62442. baseline and differential.
GenevestigatoriQ62442.

Interactioni

Subunit structurei

Interacts with VAPA and VAPB.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ62442.
SMRiQ62442. Positions 30-94.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 9361v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5143.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ62442.
KOiK08510.
OrthoDBiEOG7MSMRJ.
PhylomeDBiQ62442.
TreeFamiTF313666.

Family and domain databases

InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP_met/fun.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q62442-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAPAQPPAE GTEGAAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIMRV
60 70 80 90 100
NVDKVLERDQ KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM
110
LGAICAIIVV VIVIYFFT
Length:118
Mass (Da):12,890
Last modified:November 1, 1996 - v1
Checksum:i31CB8701446A7FB0
GO
Isoform 2 (identifier: Q62442-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYFFT → SKYR

Show »
Length:117
Mass (Da):12,753
Checksum:iA65589318A7FB071
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei114 – 1185IYFFT → SKYR in isoform 2. 1 PublicationVSP_029186

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61751 mRNA. Translation: AAB03491.1.
AF007167 mRNA. Translation: AAB62930.1.
AK018783 mRNA. Translation: BAB31407.1.
AK078156 mRNA. Translation: BAC37151.1.
BC049902 mRNA. Translation: AAH49902.2.
BC057587 mRNA. Translation: AAH57587.1.
CCDSiCCDS20546.1. [Q62442-1]
CCDS39637.1. [Q62442-2]
RefSeqiNP_001074026.1. NM_001080557.1. [Q62442-2]
NP_033522.1. NM_009496.3. [Q62442-1]
UniGeneiMm.32321.

Genome annotation databases

EnsembliENSMUST00000032487; ENSMUSP00000032487; ENSMUSG00000030337. [Q62442-1]
ENSMUST00000100942; ENSMUSP00000098503; ENSMUSG00000030337. [Q62442-2]
GeneIDi22317.
KEGGimmu:22317.
UCSCiuc009dtx.1. mouse. [Q62442-1]
uc009dty.1. mouse. [Q62442-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61751 mRNA. Translation: AAB03491.1.
AF007167 mRNA. Translation: AAB62930.1.
AK018783 mRNA. Translation: BAB31407.1.
AK078156 mRNA. Translation: BAC37151.1.
BC049902 mRNA. Translation: AAH49902.2.
BC057587 mRNA. Translation: AAH57587.1.
CCDSiCCDS20546.1. [Q62442-1]
CCDS39637.1. [Q62442-2]
RefSeqiNP_001074026.1. NM_001080557.1. [Q62442-2]
NP_033522.1. NM_009496.3. [Q62442-1]
UniGeneiMm.32321.

3D structure databases

ProteinModelPortaliQ62442.
SMRiQ62442. Positions 30-94.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ62442.

Proteomic databases

MaxQBiQ62442.
PaxDbiQ62442.
PRIDEiQ62442.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032487; ENSMUSP00000032487; ENSMUSG00000030337. [Q62442-1]
ENSMUST00000100942; ENSMUSP00000098503; ENSMUSG00000030337. [Q62442-2]
GeneIDi22317.
KEGGimmu:22317.
UCSCiuc009dtx.1. mouse. [Q62442-1]
uc009dty.1. mouse. [Q62442-2]

Organism-specific databases

CTDi6843.
MGIiMGI:1313276. Vamp1.

Phylogenomic databases

eggNOGiCOG5143.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG006675.
InParanoidiQ62442.
KOiK08510.
OrthoDBiEOG7MSMRJ.
PhylomeDBiQ62442.
TreeFamiTF313666.

Miscellaneous databases

ChiTaRSiVamp1. mouse.
NextBioi302523.
PROiQ62442.
SOURCEiSearch...

Gene expression databases

BgeeiQ62442.
CleanExiMM_VAMP1.
ExpressionAtlasiQ62442. baseline and differential.
GenevestigatoriQ62442.

Family and domain databases

InterProiIPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP_met/fun.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Martin S., Tellam J.T., Livington C., Slot J.W., Gould G.W., James D.E.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. Olken S.K., Doerre S., Corley R.B.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/c.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Cerebellum and Medulla oblongata.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6.
    Tissue: Retina.
  5. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 34-49; 50-58 AND 62-85, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry nameiVAMP1_MOUSE
AccessioniPrimary (citable) accession number: Q62442
Secondary accession number(s): Q6PFF3, Q810K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.