Q62442 (VAMP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicle-associated membrane protein 1
Short name=VAMP-1

Alternative name(s):
Synaptobrevin-1

Gene names

Name:	Vamp1
Synonyms:	Syb1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	118 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Subunit structure	Interacts with VAPA and VAPB By similarity.
Subcellular location	Isoform 1: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass type IV membrane protein By similarity. Cell junction › synapse › synaptosome By similarity. Isoform 2: Cytoplasmic vesicle membrane; Single-pass type IV membrane protein By similarity. Cell junction › synapse › synaptosome By similarity.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	vesicle-mediated transport Inferred from electronic annotation. Source: InterPro
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay. Source: MGI synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synaptosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q62442-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q62442-2)

The sequence of this isoform differs from the canonical sequence as follows:
     114-118: IYFFT → SKYR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 118	118	Vesicle-associated membrane protein 1		PRO_0000206720
Regions
	Topological domain	1 – 96	96	Cytoplasmic Potential
	Transmembrane	97 – 116	20	Helical; Anchor for type IV membrane protein; Potential
	Topological domain	117 – 118	2	Vesicular Potential
	Domain	33 – 93	61	v-SNARE coiled-coil homology
Amino acid modifications
	Modified residue	63	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	114 – 118	5	IYFFT → SKYR in isoform 2.		VSP_029186

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 31CB8701446A7FB0	FASTA	118	12,890
	10 20 30 40 50 60 MSAPAQPPAE GTEGAAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIMRV NVDKVLERDQ 70 80 90 100 110 KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM LGAICAIIVV VIVIYFFT
	Isoform 2 [UniParc]. Checksum: A65589318A7FB071	FASTA	117	12,753
	10 20 30 40 50 60 MSAPAQPPAE GTEGAAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIMRV NVDKVLERDQ 70 80 90 100 110 KLSELDDRAD ALQAGASQFE SSAAKLKRKY WWKNCKMMIM LGAICAIIVV VIVSKYR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Martin S., Tellam J.T., Livington C., Slot J.W., Gould G.W., James D.E. Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	Olken S.K., Doerre S., Corley R.B. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: BALB/c. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Cerebellum and Medulla oblongata.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6. Tissue: Retina.
[5]	Lubec G., Kang S.U., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 34-49; 50-58 AND 62-85, MASS SPECTROMETRY. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
[6]	"Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." Vosseller K., Hansen K.C., Chalkley R.J., Trinidad J.C., Wells L., Hart G.W., Burlingame A.L. Proteomics 5:388-398(2005) [PubMed: 15648052] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U61751 mRNA. Translation: AAB03491.1. AF007167 mRNA. Translation: AAB62930.1. AK018783 mRNA. Translation: BAB31407.1. AK078156 mRNA. Translation: BAC37151.1. BC049902 mRNA. Translation: AAH49902.2. BC057587 mRNA. Translation: AAH57587.1.
IPI	IPI00761439. IPI00918069.
RefSeq	NP_001074026.1. NM_001080557.1. NP_033522.1. NM_009496.3.
UniGene	Mm.32321.
3D structure databases
ProteinModelPortal	Q62442.
SMR	Q62442. Positions 30-94.
ModBase	Search...
Protein-protein interaction databases
STRING	Q62442.
PTM databases
PhosphoSite	Q62442.
Proteomic databases
PRIDE	Q62442.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032487; ENSMUSP00000032487; ENSMUSG00000030337. ENSMUST00000100942; ENSMUSP00000098503; ENSMUSG00000030337.
GeneID	22317.
KEGG	mmu:22317.
UCSC	uc007jpe.1. mouse.
Organism-specific databases
CTD	6843.
MGI	MGI:1313276. Vamp1.
Phylogenomic databases
eggNOG	roNOG17295.
GeneTree	ENSGT00550000074449.
HOVERGEN	HBG006675.
OrthoDB	EOG43JC6B.
PhylomeDB	Q62442.
Gene expression databases
ArrayExpress	Q62442.
Bgee	Q62442.
CleanEx	MM_VAMP1.
Genevestigator	Q62442.
GermOnline	ENSMUSG00000030337. Mus musculus.
Family and domain databases
InterPro	IPR001388. Synaptobrevin. IPR016444. Synaptobrevin_met/fun. [Graphical view]
KO	K08510.
Pfam	PF00957. Synaptobrevin. 1 hit. [Graphical view]
PIRSF	PIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTS	PR00219. SYNAPTOBREVN.
PROSITE	PS00417. SYNAPTOBREVIN. 1 hit. PS50892. V_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302523.
SOURCE	Search...

Entry information

Entry name

VAMP1_MOUSE

Accession

Primary (citable) accession number: Q62442
Secondary accession number(s): Q6PFF3, Q810K6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program