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Protein

Transducin-like enhancer protein 4

Gene

Tle4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression.2 Publications

GO - Molecular functioni

  • chromatin binding Source: MGI
  • repressing transcription factor binding Source: UniProtKB
  • transcription corepressor activity Source: UniProtKB
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: MGI

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • Wnt signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.
R-MMU-3769402. Deactivation of the beta-catenin transactivating complex.
R-MMU-4641265. Repression of WNT target genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Transducin-like enhancer protein 4
Alternative name(s):
Grg-4
Groucho-related protein 4
Gene namesi
Name:Tle4
Synonyms:Grg4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:104633. Tle4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512841 – 773Transducin-like enhancer protein 4Add BLAST773

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei208PhosphoserineBy similarity1
Modified residuei212PhosphoserineBy similarity1
Modified residuei216PhosphoserineBy similarity1
Modified residuei222PhosphoserineBy similarity1
Modified residuei237N6-acetyllysineBy similarity1
Modified residuei245PhosphoserineBy similarity1
Modified residuei250Phosphoserine; by CK2Sequence analysisBy similarity1
Modified residuei265Phosphoserine; by CDK1Sequence analysis1
Modified residuei269PhosphoserineBy similarity1
Modified residuei273PhosphoserineBy similarity1
Modified residuei281N6-acetyllysineBy similarity1
Modified residuei292PhosphoserineCombined sources1
Modified residuei318PhosphothreonineCombined sources1
Modified residuei321PhosphoserineCombined sources1
Modified residuei323PhosphoserineBy similarity1
Modified residuei325PhosphothreonineCombined sources1
Modified residuei327PhosphothreonineBy similarity1
Modified residuei334PhosphothreonineBy similarity1
Modified residuei340PhosphothreonineBy similarity1
Modified residuei419PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. PAX5 binding increases phosphorylation.1 Publication
Ubiquitinated by XIAP/BIRC4.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ62441.
MaxQBiQ62441.
PaxDbiQ62441.
PRIDEiQ62441.

PTM databases

iPTMnetiQ62441.
PhosphoSitePlusiQ62441.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024642.
ExpressionAtlasiQ62441. baseline and differential.
GenevisibleiQ62441. MM.

Interactioni

Subunit structurei

Homooligomer and heterooligomer with other family members (By similarity). Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression. Interacts with SIX3 and SIX6. Interacts with PAX2 (By similarity).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Six3Q622332EBI-2297871,EBI-2297327

GO - Molecular functioni

  • repressing transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi204219. 9 interactors.
IntActiQ62441. 3 interactors.
STRINGi10090.ENSMUSP00000057527.

Structurei

3D structure databases

ProteinModelPortaliQ62441.
SMRiQ62441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati485 – 523WD 1Add BLAST39
Repeati531 – 570WD 2Add BLAST40
Repeati575 – 614WD 3Add BLAST40
Repeati617 – 656WD 4Add BLAST40
Repeati658 – 697WD 5Add BLAST40
Repeati699 – 738WD 6Add BLAST40
Repeati740 – 773WD 7Add BLAST34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 136Q domainBy similarityAdd BLAST136
Regioni137 – 204GP domainBy similarityAdd BLAST68
Regioni205 – 274CcN domainBy similarityAdd BLAST70
Regioni275 – 452SP domainBy similarityAdd BLAST178

Domaini

WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.1 Publication

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
GeneTreeiENSGT00550000074465.
HOVERGENiHBG004689.
InParanoidiQ62441.
OMAiCPYPAPF.
PhylomeDBiQ62441.
TreeFamiTF314167.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005617. Groucho/TLE_N.
IPR009146. Groucho_enhance.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR01850. GROUCHOFAMLY.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62441-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRDLSKMYP QTRHPAPHQP AQPFKFTISE SCDRIKEEFQ FLQAQYHSLK
60 70 80 90 100
LECEKLASEK TEMQRHYVMY YEMSYGLNIE MHKQAEIVKR LNAICAQVIP
110 120 130 140 150
FLSQEHQQQV VQAVERAKQV TMAELNAIIG QQLQAQHLSH GHGLPVPLTP
160 170 180 190 200
HPSGLQPPAI PPIGSSAGLL ALSSALGGQS HLPIKDEKKH HDNDHQRDRD
210 220 230 240 250
SIKSSSVSPS ASFRGSEKHR NSTDYSSESK KQKTEEKEIA ARYDSDGEKS
260 270 280 290 300
DDNLVVDVSN EDPSSPRGSP AHSPRENGLD KTRLLKKDAP ISPASVASSS
310 320 330 340 350
STPSSKSKEL SLNEKSTTPV SKSNTPTPRT DAPTPGSNST PGLRPVPGKP
360 370 380 390 400
PGVDPLASSL RTPMAVPCPY PTPFGIVPHA GMNGELTSPG AAYAGLHNIS
410 420 430 440 450
PQMSAAAAAA AAAAAYGRSP VVGFDPHHHM RVPAIPPNLT GIPGGKPAYS
460 470 480 490 500
FHVSADGQMQ PVPFPPDALI GPGIPRHARQ INTLNHGEVV CAVTISNPTR
510 520 530 540 550
HVYTGGKGCV KVWDISHPGN KSPVSQLDCL NRDNYIRSCR LLPDGRTLIV
560 570 580 590 600
GGEASTLSIW DLAAPTPRIK AELTSSAPAC YALAISPDSK VCFSCCSDGN
610 620 630 640 650
IAVWDLHNQT LVRQFQGHTD GASCIDISND GTKLWTGGLD NTVRSWDLRE
660 670 680 690 700
GRQLQQHDFT SQIFSLGYCP TGEWLAVGME NSNVEVLHVT KPDKYQLHLH
710 720 730 740 750
ESCVLSLKFA HCGKWFVSTG KDNLLNAWRT PYGASIFQSK ESSSVLSCDI
760 770
SVDDKYIVTG SGDKKATVYE VIY
Length:773
Mass (Da):83,787
Last modified:September 11, 2007 - v4
Checksum:i5A0090789774CACC
GO

Sequence cautioni

The sequence AAF43203 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197 – 199RDR → QTN in AAC97070 (PubMed:8892234).Curated3
Sequence conflicti405A → R in AAC97070 (PubMed:8892234).Curated1
Sequence conflicti454 – 455SA → RS in AAC97070 (PubMed:8892234).Curated2
Sequence conflicti608 – 609NQ → KE in AAC97070 (PubMed:8892234).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229633 mRNA. Translation: AAF43203.1. Different initiation.
U61363 mRNA. Translation: AAC97070.1.
CCDSiCCDS50399.1.
RefSeqiNP_035730.2. NM_011600.3.
UniGeneiMm.103638.

Genome annotation databases

EnsembliENSMUST00000052011; ENSMUSP00000057527; ENSMUSG00000024642.
GeneIDi21888.
KEGGimmu:21888.
UCSCiuc008gwk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF229633 mRNA. Translation: AAF43203.1. Different initiation.
U61363 mRNA. Translation: AAC97070.1.
CCDSiCCDS50399.1.
RefSeqiNP_035730.2. NM_011600.3.
UniGeneiMm.103638.

3D structure databases

ProteinModelPortaliQ62441.
SMRiQ62441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204219. 9 interactors.
IntActiQ62441. 3 interactors.
STRINGi10090.ENSMUSP00000057527.

PTM databases

iPTMnetiQ62441.
PhosphoSitePlusiQ62441.

Proteomic databases

EPDiQ62441.
MaxQBiQ62441.
PaxDbiQ62441.
PRIDEiQ62441.

Protocols and materials databases

DNASUi21888.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052011; ENSMUSP00000057527; ENSMUSG00000024642.
GeneIDi21888.
KEGGimmu:21888.
UCSCiuc008gwk.2. mouse.

Organism-specific databases

CTDi7091.
MGIiMGI:104633. Tle4.

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
GeneTreeiENSGT00550000074465.
HOVERGENiHBG004689.
InParanoidiQ62441.
OMAiCPYPAPF.
PhylomeDBiQ62441.
TreeFamiTF314167.

Enzyme and pathway databases

ReactomeiR-MMU-201722. Formation of the beta-catenin:TCF transactivating complex.
R-MMU-3769402. Deactivation of the beta-catenin transactivating complex.
R-MMU-4641265. Repression of WNT target genes.

Miscellaneous databases

PROiQ62441.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024642.
ExpressionAtlasiQ62441. baseline and differential.
GenevisibleiQ62441. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR005617. Groucho/TLE_N.
IPR009146. Groucho_enhance.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03920. TLE_N. 1 hit.
PF00400. WD40. 2 hits.
[Graphical view]
PRINTSiPR01850. GROUCHOFAMLY.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLE4_MOUSE
AccessioniPrimary (citable) accession number: Q62441
Secondary accession number(s): Q9JKQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 11, 2007
Last modified: November 2, 2016
This is version 141 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-8 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.