Reviewed, UniProtKB/Swiss-Prot Q62441 (TLE4_MOUSE)

Names and origin

Protein names

Recommended name:
    Transducin-like enhancer protein 4
Alternative name(s):
    Groucho-related protein 4
    Grg-4

Gene names

Name:	Tle4
Synonyms:	Grg4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	773 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES By similarity.
Subunit structure	Homooligomer and heterooligomer with other family members By similarity. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2.
Subcellular location	Nucleus Probable.
Post-translational modification	Phosphorylated. PAX5 binding increases phosphorylation. Ref.1
Sequence similarities	Belongs to the WD repeat Groucho/TLE family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Wnt signaling pathway
Cellular component	Nucleus
Domain	Repeat WD repeat
Molecular function	Repressor
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from direct assay. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred by curator. Source: MGI
Molecular function	RNA polymerase II transcription factor activity, enhancer binding Inferred from genetic interaction. Source: MGI chromatin binding Inferred from direct assay. Source: MGI transcription corepressor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 773	773	Transducin-like enhancer protein 4		PRO_0000051284
Regions
	Repeat	485 – 523	39	WD 1
	Repeat	531 – 570	40	WD 2
	Repeat	575 – 614	40	WD 3
	Repeat	617 – 656	40	WD 4
	Repeat	658 – 697	40	WD 5
	Repeat	699 – 738	40	WD 6
	Repeat	740 – 773	34	WD 7
	Region	207 – 274	68	CCN domain
	Compositional bias	1 – 137	137	Gln-rich (Q domain)
	Compositional bias	138 – 205	68	Gly/Pro-rich (GP domain)
Amino acid modifications
	Modified residue	250	1	Phosphoserine; by CK2 Potential
	Modified residue	265	1	Phosphoserine; by CDC2 Potential
Experimental info
	Sequence conflict	197 – 199	3	RDR → QTN in AAC97070. Ref.2
	Sequence conflict	405	1	A → R in AAC97070. Ref.2
	Sequence conflict	454 – 455	2	SA → RS in AAC97070. Ref.2
	Sequence conflict	608 – 609	2	NQ → KE in AAC97070. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q62441-1 [UniParc]. Last modified September 11, 2007. Version 4. Checksum: 5A0090789774CACC	FASTA	773	83,787
	10 20 30 40 50 60 MIRDLSKMYP QTRHPAPHQP AQPFKFTISE SCDRIKEEFQ FLQAQYHSLK LECEKLASEK 70 80 90 100 110 120 TEMQRHYVMY YEMSYGLNIE MHKQAEIVKR LNAICAQVIP FLSQEHQQQV VQAVERAKQV 130 140 150 160 170 180 TMAELNAIIG QQLQAQHLSH GHGLPVPLTP HPSGLQPPAI PPIGSSAGLL ALSSALGGQS 190 200 210 220 230 240 HLPIKDEKKH HDNDHQRDRD SIKSSSVSPS ASFRGSEKHR NSTDYSSESK KQKTEEKEIA 250 260 270 280 290 300 ARYDSDGEKS DDNLVVDVSN EDPSSPRGSP AHSPRENGLD KTRLLKKDAP ISPASVASSS 310 320 330 340 350 360 STPSSKSKEL SLNEKSTTPV SKSNTPTPRT DAPTPGSNST PGLRPVPGKP PGVDPLASSL 370 380 390 400 410 420 RTPMAVPCPY PTPFGIVPHA GMNGELTSPG AAYAGLHNIS PQMSAAAAAA AAAAAYGRSP 430 440 450 460 470 480 VVGFDPHHHM RVPAIPPNLT GIPGGKPAYS FHVSADGQMQ PVPFPPDALI GPGIPRHARQ 490 500 510 520 530 540 INTLNHGEVV CAVTISNPTR HVYTGGKGCV KVWDISHPGN KSPVSQLDCL NRDNYIRSCR 550 560 570 580 590 600 LLPDGRTLIV GGEASTLSIW DLAAPTPRIK AELTSSAPAC YALAISPDSK VCFSCCSDGN 610 620 630 640 650 660 IAVWDLHNQT LVRQFQGHTD GASCIDISND GTKLWTGGLD NTVRSWDLRE GRQLQQHDFT 670 680 690 700 710 720 SQIFSLGYCP TGEWLAVGME NSNVEVLHVT KPDKYQLHLH ESCVLSLKFA HCGKWFVSTG 730 740 750 760 770 KDNLLNAWRT PYGASIFQSK ESSSVLSCDI SVDDKYIVTG SGDKKATVYE VIY

References

[1]	"Transcriptional repression by Pax5 (BSAP) through interaction with corepressors of the Groucho family." Eberhard D., Jimenez G., Heavey B., Busslinger M. EMBO J. 19:2292-2303(2000) [PubMed: 10811620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION, INTERACTION WITH PAX5. Tissue: Embryo.
[2]	"Transcripts of Grg4, a murine groucho-related gene, are detected in adjacent tissues to other murine neurogenic gene homologues during embryonic development." Koop K.E., Macdonald L.M., Lobe C.G. Mech. Dev. 59:73-87(1996) [PubMed: 8892234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 197-773.
[3]	Lobe C.G., Koop K.E., Macdonald L.M. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"All Tcf HMG box transcription factors interact with Groucho-related co-repressors." Brantjes H., Roose J., van De Wetering M., Clevers H. Nucleic Acids Res. 29:1410-1419(2001) [PubMed: 11266540] [Abstract] Cited for: INTERACTION WITH LEF1; TCF7; TCF7L1 AND TCF7L2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF229633 mRNA. Translation: AAF43203.1. Different initiation. U61363 mRNA. Translation: AAC97070.1.
IPI	IPI00407702.
RefSeq	NP_035730.2.
UniGene	Mm.103638
3D structure databases
HSSP	HSSP built from PDB template 1GXR based on UniProtKB Q04724.
SMR	Q62441. Positions 434-766.
ModBase	Search...
PTM databases
PhosphoSite	Q62441.
Proteomic databases
PRIDE	Q62441.
Genome annotation databases
Ensembl	ENSMUSG00000024642. Mus musculus. [Contig view]
GeneID	21888.
KEGG	mmu:21888.
Organism-specific databases
MGI	MGI:104633. Tle4.
Phylogenomic databases
HOVERGEN	Q62441.
Gene expression databases
ArrayExpress	Q62441.
Bgee	Q62441.
GermOnline	ENSMUSG00000024642. Mus musculus.
Family and domain databases
InterPro	IPR009146. Groucho_enhance. IPR005617. TLE_N. IPR015943. WD40/YVTN_repeat-like. IPR001680. WD40_repeat. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_region. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF03920. TLE_N. 1 hit. PF00400. WD40. 6 hits. [Graphical view]
PRINTS	PR01850. GROUCHOFAMLY.
ProDom	PD000018. WD40. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00320. WD40. 7 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	301416.
SOURCE	Search...

Entry information

Entry name

TLE4_MOUSE

Accession

Primary (citable) accession number: Q62441
Secondary accession number(s): Q9JKQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	September 11, 2007
Last modified:	June 16, 2009
This is version 76 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)