Q62441 (TLE4_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 103.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Transducin-like enhancer protein 4 Alternative name(s): Grg-4 Groucho-related protein 4 | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 773 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Ref.5 |
| Subunit structure | Homooligomer and heterooligomer with other family members By similarity. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression. Ref.1 Ref.4 Ref.5 |
| Subcellular location | Nucleus Probable. |
| Post-translational modification | Phosphorylated. PAX5 binding increases phosphorylation. Ref.1 |
| Sequence similarities | Belongs to the WD repeat Groucho/TLE family. Contains 7 WD repeats. |
| Sequence caution | The sequence AAF43203.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation Wnt signaling pathway |
| Cellular component | Nucleus |
| Domain | Repeat WD repeat |
| Molecular function | Repressor |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | Wnt receptor signaling pathway Inferred from direct assay. Source: MGI negative regulation of transcription from RNA polymerase II promoterInferred from direct assay. Source: MGI transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | nucleus Inferred by curator. Source: MGI |
| Molecular function | chromatin binding Inferred from direct assay. Source: MGI repressing transcription factor bindingInferred from physical interaction Ref.5. Source: UniProtKB transcription corepressor activityInferred from direct assay Ref.5. Source: UniProtKB |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Six3 | Q62233 | 2 | EBI-2297871,EBI-2297327 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 773 | 773 | Transducin-like enhancer protein 4 | PRO_0000051284 | |||||
Regions | |||||||||
| Repeat | 485 – 523 | 39 | WD 1 | ||||||
| Repeat | 531 – 570 | 40 | WD 2 | ||||||
| Repeat | 575 – 614 | 40 | WD 3 | ||||||
| Repeat | 617 – 656 | 40 | WD 4 | ||||||
| Repeat | 658 – 697 | 40 | WD 5 | ||||||
| Repeat | 699 – 738 | 40 | WD 6 | ||||||
| Repeat | 740 – 773 | 34 | WD 7 | ||||||
| Region | 207 – 274 | 68 | CCN domain | ||||||
| Compositional bias | 1 – 137 | 137 | Gln-rich (Q domain) | ||||||
| Compositional bias | 138 – 205 | 68 | Gly/Pro-rich (GP domain) | ||||||
Amino acid modifications | |||||||||
| Modified residue | 206 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 208 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 237 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 243 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 245 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 250 | 1 | Phosphoserine; by CK2 Potential | ||||||
| Modified residue | 265 | 1 | Phosphoserine; by CDK1 Potential | ||||||
| Modified residue | 281 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 292 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 305 | 1 | Phosphoserine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 197 – 199 | 3 | RDR → QTN in AAC97070. Ref.2 | ||||||
| Sequence conflict | 405 | 1 | A → R in AAC97070. Ref.2 | ||||||
| Sequence conflict | 454 – 455 | 2 | SA → RS in AAC97070. Ref.2 | ||||||
| Sequence conflict | 608 – 609 | 2 | NQ → KE in AAC97070. Ref.2 | ||||||
Sequences
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References
| [1] | "Transcriptional repression by Pax5 (BSAP) through interaction with corepressors of the Groucho family." Eberhard D., Jimenez G., Heavey B., Busslinger M. EMBO J. 19:2292-2303(2000) [PubMed: 10811620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION, INTERACTION WITH PAX5. Tissue: Embryo. |
| [2] | "Transcripts of Grg4, a murine groucho-related gene, are detected in adjacent tissues to other murine neurogenic gene homologues during embryonic development." Koop K.E., Macdonald L.M., Lobe C.G. Mech. Dev. 59:73-87(1996) [PubMed: 8892234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 197-773. |
| [3] | Lobe C.G., Koop K.E., Macdonald L.M. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. |
| [4] | "All Tcf HMG box transcription factors interact with Groucho-related co-repressors." Brantjes H., Roose J., van De Wetering M., Clevers H. Nucleic Acids Res. 29:1410-1419(2001) [PubMed: 11266540] [Abstract] Cited for: INTERACTION WITH LEF1; TCF7; TCF7L1 AND TCF7L2. |
| [5] | "Zeppo1 is a novel metastasis promoter that represses E-cadherin expression and regulates p120-catenin isoform expression and localization." Slorach E.M., Chou J., Werb Z. Genes Dev. 25:471-484(2011) [PubMed: 21317240] [Abstract] Cited for: FUNCTION, INTERACTION WITH ZNF703. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF229633 mRNA. Translation: AAF43203.1. Different initiation. U61363 mRNA. Translation: AAC97070.1. |
| IPI | IPI00407702. |
| RefSeq | NP_035730.2. NM_011600.2. |
| UniGene | Mm.103638. |
3D structure databases | |
| ProteinModelPortal | Q62441. |
| SMR | Q62441. Positions 441-773. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q62441. 3 interactions. |
| STRING | Q62441. |
PTM databases | |
| PhosphoSite | Q62441. |
Proteomic databases | |
| PRIDE | Q62441. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000052011; ENSMUSP00000057527; ENSMUSG00000024642. |
| GeneID | 21888. |
| KEGG | mmu:21888. |
Organism-specific databases | |
| CTD | 7091. |
| MGI | MGI:104633. Tle4. |
Phylogenomic databases | |
| HOVERGEN | HBG004689. |
| InParanoid | Q62441. |
| OrthoDB | EOG4MGS6V. |
Gene expression databases | |
| ArrayExpress | Q62441. |
| Bgee | Q62441. |
| Genevestigator | Q62441. |
| GermOnline | ENSMUSG00000024642. Mus musculus. |
Family and domain databases | |
| InterPro | IPR005617. Groucho/TLE_N. IPR009146. Groucho_enhance. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view] |
| Gene3D | G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit. |
| Pfam | PF03920. TLE_N. 1 hit. PF00400. WD40. 6 hits. [Graphical view] |
| PRINTS | PR01850. GROUCHOFAMLY. |
| SMART | SM00320. WD40. 7 hits. [Graphical view] |
| SUPFAM | SSF50978. WD40_like. 1 hit. |
| PROSITE | PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 301416. |
| SOURCE | Search... |
Entry information
| Entry name | TLE4_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q62441 Secondary accession number(s): Q9JKQ9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with