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Q62441 (TLE4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transducin-like enhancer protein 4
Alternative name(s):
Grg-4
Groucho-related protein 4
Gene names
Name:Tle4
Synonyms:Grg4
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length773 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Ref.5

Subunit structure

Homooligomer and heterooligomer with other family members By similarity. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression. Ref.1 Ref.4 Ref.5

Subcellular location

Nucleus Probable.

Post-translational modification

Phosphorylated. PAX5 binding increases phosphorylation. Ref.1

Sequence similarities

Belongs to the WD repeat Groucho/TLE family.

Contains 7 WD repeats.

Sequence caution

The sequence AAF43203.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Six3Q622332EBI-2297871,EBI-2297327

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 773773Transducin-like enhancer protein 4
PRO_0000051284

Regions

Repeat485 – 52339WD 1
Repeat531 – 57040WD 2
Repeat575 – 61440WD 3
Repeat617 – 65640WD 4
Repeat658 – 69740WD 5
Repeat699 – 73840WD 6
Repeat740 – 77334WD 7
Region207 – 27468CCN domain
Compositional bias1 – 137137Gln-rich (Q domain)
Compositional bias138 – 20568Gly/Pro-rich (GP domain)

Amino acid modifications

Modified residue2061Phosphoserine By similarity
Modified residue2081Phosphoserine By similarity
Modified residue2371N6-acetyllysine By similarity
Modified residue2431Phosphotyrosine By similarity
Modified residue2451Phosphoserine By similarity
Modified residue2501Phosphoserine; by CK2 Potential
Modified residue2651Phosphoserine; by CDK1 Potential
Modified residue2811N6-acetyllysine By similarity
Modified residue2921Phosphoserine By similarity
Modified residue3051Phosphoserine By similarity

Experimental info

Sequence conflict197 – 1993RDR → QTN in AAC97070. Ref.2
Sequence conflict4051A → R in AAC97070. Ref.2
Sequence conflict454 – 4552SA → RS in AAC97070. Ref.2
Sequence conflict608 – 6092NQ → KE in AAC97070. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q62441 [UniParc].

Last modified September 11, 2007. Version 4.
Checksum: 5A0090789774CACC

FASTA77383,787
        10         20         30         40         50         60 
MIRDLSKMYP QTRHPAPHQP AQPFKFTISE SCDRIKEEFQ FLQAQYHSLK LECEKLASEK 

        70         80         90        100        110        120 
TEMQRHYVMY YEMSYGLNIE MHKQAEIVKR LNAICAQVIP FLSQEHQQQV VQAVERAKQV 

       130        140        150        160        170        180 
TMAELNAIIG QQLQAQHLSH GHGLPVPLTP HPSGLQPPAI PPIGSSAGLL ALSSALGGQS 

       190        200        210        220        230        240 
HLPIKDEKKH HDNDHQRDRD SIKSSSVSPS ASFRGSEKHR NSTDYSSESK KQKTEEKEIA 

       250        260        270        280        290        300 
ARYDSDGEKS DDNLVVDVSN EDPSSPRGSP AHSPRENGLD KTRLLKKDAP ISPASVASSS 

       310        320        330        340        350        360 
STPSSKSKEL SLNEKSTTPV SKSNTPTPRT DAPTPGSNST PGLRPVPGKP PGVDPLASSL 

       370        380        390        400        410        420 
RTPMAVPCPY PTPFGIVPHA GMNGELTSPG AAYAGLHNIS PQMSAAAAAA AAAAAYGRSP 

       430        440        450        460        470        480 
VVGFDPHHHM RVPAIPPNLT GIPGGKPAYS FHVSADGQMQ PVPFPPDALI GPGIPRHARQ 

       490        500        510        520        530        540 
INTLNHGEVV CAVTISNPTR HVYTGGKGCV KVWDISHPGN KSPVSQLDCL NRDNYIRSCR 

       550        560        570        580        590        600 
LLPDGRTLIV GGEASTLSIW DLAAPTPRIK AELTSSAPAC YALAISPDSK VCFSCCSDGN 

       610        620        630        640        650        660 
IAVWDLHNQT LVRQFQGHTD GASCIDISND GTKLWTGGLD NTVRSWDLRE GRQLQQHDFT 

       670        680        690        700        710        720 
SQIFSLGYCP TGEWLAVGME NSNVEVLHVT KPDKYQLHLH ESCVLSLKFA HCGKWFVSTG 

       730        740        750        760        770 
KDNLLNAWRT PYGASIFQSK ESSSVLSCDI SVDDKYIVTG SGDKKATVYE VIY

« Hide

References

[1]"Transcriptional repression by Pax5 (BSAP) through interaction with corepressors of the Groucho family."
Eberhard D., Jimenez G., Heavey B., Busslinger M.
EMBO J. 19:2292-2303(2000) [PubMed: 10811620] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION, INTERACTION WITH PAX5.
Tissue: Embryo.
[2]"Transcripts of Grg4, a murine groucho-related gene, are detected in adjacent tissues to other murine neurogenic gene homologues during embryonic development."
Koop K.E., Macdonald L.M., Lobe C.G.
Mech. Dev. 59:73-87(1996) [PubMed: 8892234] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 197-773.
[3]Lobe C.G., Koop K.E., Macdonald L.M.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[4]"All Tcf HMG box transcription factors interact with Groucho-related co-repressors."
Brantjes H., Roose J., van De Wetering M., Clevers H.
Nucleic Acids Res. 29:1410-1419(2001) [PubMed: 11266540] [Abstract]
Cited for: INTERACTION WITH LEF1; TCF7; TCF7L1 AND TCF7L2.
[5]"Zeppo1 is a novel metastasis promoter that represses E-cadherin expression and regulates p120-catenin isoform expression and localization."
Slorach E.M., Chou J., Werb Z.
Genes Dev. 25:471-484(2011) [PubMed: 21317240] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ZNF703.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF229633 mRNA. Translation: AAF43203.1. Different initiation.
U61363 mRNA. Translation: AAC97070.1.
IPIIPI00407702.
RefSeqNP_035730.2. NM_011600.2.
UniGeneMm.103638.

3D structure databases

ProteinModelPortalQ62441.
SMRQ62441. Positions 441-773.
ModBaseSearch...

Protein-protein interaction databases

IntActQ62441. 3 interactions.
STRINGQ62441.

PTM databases

PhosphoSiteQ62441.

Proteomic databases

PRIDEQ62441.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000052011; ENSMUSP00000057527; ENSMUSG00000024642.
GeneID21888.
KEGGmmu:21888.

Organism-specific databases

CTD7091.
MGIMGI:104633. Tle4.

Phylogenomic databases

HOVERGENHBG004689.
InParanoidQ62441.
OrthoDBEOG4MGS6V.

Gene expression databases

ArrayExpressQ62441.
BgeeQ62441.
GenevestigatorQ62441.
GermOnlineENSMUSG00000024642. Mus musculus.

Family and domain databases

InterProIPR005617. Groucho/TLE_N.
IPR009146. Groucho_enhance.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
Gene3DG3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PfamPF03920. TLE_N. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSPR01850. GROUCHOFAMLY.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. WD40_like. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio301416.
SOURCESearch...

Entry information

Entry nameTLE4_MOUSE
AccessionPrimary (citable) accession number: Q62441
Secondary accession number(s): Q9JKQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 11, 2007
Last modified: January 25, 2012
This is version 103 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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