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Protein

Protein NDRG1

Gene

Ndrg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy (By similarity). Has a role in cell trafficking notably of the Schwann cell and is necessary for the maintenance and development of the peripheral nerve myelin sheath.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • cellular response to hypoxia Source: Ensembl
  • DNA damage response, signal transduction by p53 class mediator Source: Ensembl
  • mast cell activation Source: MGI
  • negative regulation of cell proliferation Source: CACAO
  • peripheral nervous system myelin maintenance Source: UniProtKB
  • positive regulation of spindle checkpoint Source: Ensembl
Complete GO annotation...

Protein family/group databases

ESTHERimouse-ndr1. Ndr_family.
MEROPSiS33.988.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein NDRG1
Alternative name(s):
N-myc downstream-regulated gene 1 protein
Short name:
Protein Ndr1
Gene namesi
Name:Ndrg1
Synonyms:Ndr1, Ndrl, Tdd5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1341799. Ndrg1.

Subcellular locationi

  • Cytoplasmcytosol
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome
  • Nucleus
  • Cell membrane By similarity

  • Note: Mainly cytoplasmic but differentially localized to other regions. Associates with the plasma membrane in intestinal epithelia and lactating mammary gland. Translocated to the nucleus in a p53/TP53-dependent manner. In prostate epithelium and placental chorion, located in both the cytoplasm and in the nucleus. No nuclear localization in colon epithelium cells. In intestinal mucosa, prostate and renal cortex, located predominantly adjacent to adherens junctions. Cytoplasmic with granular staining in proximal tubular cells of the kidney and salivary gland ducts. Recruits to the membrane of recycling/sorting and late endosomes via binding to phosphatidylinositol 4-phosphate. Associates with microtubules. Colocalizes with TUBG1 in the centrosome. Cytoplasmic location increased with hypoxia. Phosphorylated form found associated with centromeres during S-phase of mitosis and with the plasma membrane (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Microtubule, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mutant mice exhibit defects in peripheral nerve development. Initial hind limb weakness developed around age 12 weeks, and significant functional impairment (dragging of hind legs) and muscle atrophy became apparent at age 1 year. After about 5 weeks extensive demyelination of nerve fibers is observed. In later life, large inclusions were seen in the adaxonal Schwann cell cytoplasm. There is no evidence of apoptotic response.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 394393Protein NDRG1PRO_0000159574Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei2 – 21PhosphoserineBy similarity
Modified residuei319 – 3191PhosphoserineCombined sources
Modified residuei326 – 3261PhosphoserineCombined sources
Modified residuei328 – 3281PhosphothreonineCombined sources
Modified residuei330 – 3301Phosphoserine; by SGK1Combined sources
Modified residuei332 – 3321PhosphoserineCombined sources
Modified residuei333 – 3331PhosphoserineCombined sources
Modified residuei335 – 3351PhosphothreonineCombined sources
Modified residuei336 – 3361PhosphoserineCombined sources
Modified residuei340 – 3401PhosphothreonineCombined sources
Modified residuei342 – 3421PhosphoserineCombined sources
Modified residuei346 – 3461Phosphothreonine; by SGK11 Publication
Modified residuei352 – 3521PhosphoserineBy similarity
Modified residuei356 – 3561Phosphothreonine; by SGK1By similarity
Modified residuei362 – 3621PhosphoserineBy similarity
Modified residuei364 – 3641PhosphoserineBy similarity
Modified residuei366 – 3661Phosphothreonine; by SGK1By similarity
Modified residuei375 – 3751PhosphothreonineBy similarity

Post-translational modificationi

Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ62433.
MaxQBiQ62433.
PaxDbiQ62433.
PRIDEiQ62433.

PTM databases

iPTMnetiQ62433.
PhosphoSiteiQ62433.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in kidney followed by brain, pancreas, small intestine, colon and spleen (at protein level). Also detected in heart and preputial gland, and in much smaller quantities in other tissues. Not detected in duodenum and prostate. Highly expressed in Schwann cells.4 Publications

Developmental stagei

In early stages of embryo development, expression low when MYCN expression is high. Later, when MYCN levels diminish, levels increase.1 Publication

Inductioni

Repressed by testosterone and also to a lesser extent by dihydrotestosterone. Down-regulated by MYCN.2 Publications

Gene expression databases

BgeeiQ62433.
CleanExiMM_NDRG1.
ExpressionAtlasiQ62433. baseline and differential.
GenevisibleiQ62433. MM.

Interactioni

Subunit structurei

Interacts with RAB4A (membrane-bound form); the interaction involves NDRG1 in vesicular recycling of CDH1. Interacts with APOA1, APOA2, PRA1 and RTN1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201714. 1 interaction.
IntActiQ62433. 6 interactions.
MINTiMINT-1849784.
STRINGi10090.ENSMUSP00000005256.

Structurei

3D structure databases

ProteinModelPortaliQ62433.
SMRiQ62433. Positions 33-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati339 – 348101
Repeati349 – 358102
Repeati359 – 368103

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni339 – 368303 X 10 AA tandem repeats of G-[PST]-R-S-R-S-H-T-S-EAdd
BLAST

Sequence similaritiesi

Belongs to the NDRG family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2931. Eukaryota.
ENOG410XSPF. LUCA.
GeneTreeiENSGT00390000001874.
HOGENOMiHOG000230891.
HOVERGENiHBG052591.
InParanoidiQ62433.
KOiK18266.
OMAiKTCFNPL.
OrthoDBiEOG7KH9JS.
PhylomeDBiQ62433.
TreeFamiTF313168.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR004142. NDRG.
IPR030693. NDRG1.
[Graphical view]
PANTHERiPTHR11034. PTHR11034. 1 hit.
PTHR11034:SF18. PTHR11034:SF18. 1 hit.
PfamiPF03096. Ndr. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62433-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRELHDVDL AEVKPLVEKG ESITGLLQEF DVQEQDIETL HGSLHVTLCG
60 70 80 90 100
TPKGNRPVIL TYHDIGMNHK TCYNPLFNSE DMQEITQHFA VCHVDAPGQQ
110 120 130 140 150
DGAPSFPVGY MYPSMDQLAE MLPGVLHQFG LKSVIGMGTG AGAYILTRFA
160 170 180 190 200
LNNPEMVEGL VLMNVNPCAE GWMDWAASKI SGWTQALPDM VVSHLFGKEE
210 220 230 240 250
IHNNVEVVHT YRQHILNDMN PSNLHLFISA YNSRRDLEIE RPMPGTHTVT
260 270 280 290 300
LQCPALLVVG DNSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK
310 320 330 340 350
LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLEGT RSRSHTSEGP
360 370 380 390
RSRSHTSEGS RSRSHTSEDA RLNITPNSGA TGNNAGPKSM EVSC
Length:394
Mass (Da):43,009
Last modified:November 1, 1996 - v1
Checksum:i905CA71ECF4C87C2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 353QEQ → LEE in AAB58249 (PubMed:9144177).Curated
Sequence conflicti89 – 891F → P in AAB58249 (PubMed:9144177).Curated
Sequence conflicti103 – 1097APSFPVG → PLPSQW in AAB58249 (PubMed:9144177).Curated
Sequence conflicti141 – 1488AGAYILTR → PWXLHPDP in AAB58249 (PubMed:9144177).Curated
Sequence conflicti191 – 20818VVSHL…NVEVV → CVPPLRXGGDTQQRGGM in AAB58249 (PubMed:9144177).CuratedAdd
BLAST
Sequence conflicti241 – 2411R → A in AAB58249 (PubMed:9144177).Curated
Sequence conflicti298 – 35053PAKLA…TSEGP → RPSLLRPSSTLCRHGIHAFC QHDSPDRVPHPVWLQCHILE GT in AAB58249 (PubMed:9144177).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60593 mRNA. Translation: AAB03484.1.
U52073 mRNA. Translation: AAB58249.1.
BC015282 mRNA. Translation: AAH15282.1.
BC071235 mRNA. Translation: AAH71235.1.
CCDSiCCDS37092.1.
RefSeqiNP_032707.2. NM_008681.2.
UniGeneiMm.30837.

Genome annotation databases

EnsembliENSMUST00000005256; ENSMUSP00000005256; ENSMUSG00000005125.
GeneIDi17988.
KEGGimmu:17988.
UCSCiuc007wax.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60593 mRNA. Translation: AAB03484.1.
U52073 mRNA. Translation: AAB58249.1.
BC015282 mRNA. Translation: AAH15282.1.
BC071235 mRNA. Translation: AAH71235.1.
CCDSiCCDS37092.1.
RefSeqiNP_032707.2. NM_008681.2.
UniGeneiMm.30837.

3D structure databases

ProteinModelPortaliQ62433.
SMRiQ62433. Positions 33-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201714. 1 interaction.
IntActiQ62433. 6 interactions.
MINTiMINT-1849784.
STRINGi10090.ENSMUSP00000005256.

Protein family/group databases

ESTHERimouse-ndr1. Ndr_family.
MEROPSiS33.988.

PTM databases

iPTMnetiQ62433.
PhosphoSiteiQ62433.

Proteomic databases

EPDiQ62433.
MaxQBiQ62433.
PaxDbiQ62433.
PRIDEiQ62433.

Protocols and materials databases

DNASUi17988.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005256; ENSMUSP00000005256; ENSMUSG00000005125.
GeneIDi17988.
KEGGimmu:17988.
UCSCiuc007wax.1. mouse.

Organism-specific databases

CTDi10397.
MGIiMGI:1341799. Ndrg1.

Phylogenomic databases

eggNOGiKOG2931. Eukaryota.
ENOG410XSPF. LUCA.
GeneTreeiENSGT00390000001874.
HOGENOMiHOG000230891.
HOVERGENiHBG052591.
InParanoidiQ62433.
KOiK18266.
OMAiKTCFNPL.
OrthoDBiEOG7KH9JS.
PhylomeDBiQ62433.
TreeFamiTF313168.

Miscellaneous databases

ChiTaRSiNdrg1. mouse.
PROiQ62433.
SOURCEiSearch...

Gene expression databases

BgeeiQ62433.
CleanExiMM_NDRG1.
ExpressionAtlasiQ62433. baseline and differential.
GenevisibleiQ62433. MM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR004142. NDRG.
IPR030693. NDRG1.
[Graphical view]
PANTHERiPTHR11034. PTHR11034. 1 hit.
PTHR11034:SF18. PTHR11034:SF18. 1 hit.
PfamiPF03096. Ndr. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "N-myc-dependent repression of ndr1, a gene identified by direct subtraction of whole mouse embryo cDNAs between wild type and N-myc mutant."
    Shimono A., Okuda T., Kondoh H.
    Mech. Dev. 83:39-52(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION.
    Tissue: Embryo.
  2. "Cloning and characterization of TDD5, an androgen target gene that is differentially repressed by testosterone and dihydrotestosterone."
    Lin T.-M., Chang C.
    Proc. Natl. Acad. Sci. U.S.A. 94:4988-4993(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Tissue: Hybridoma.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  4. "Exploitation of KESTREL to identify NDRG family members as physiological substrates for SGK1 and GSK3."
    Murray J.T., Campbell D.G., Morrice N., Auld G.C., Shpiro N., Marquez R., Peggie M., Bain J., Bloomberg G.B., Grahammer F., Lang F., Wulff P., Kuhl D., Cohen P.
    Biochem. J. 384:477-488(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-346; THR-356 AND THR-366, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Ndrg1-deficient mice exhibit a progressive demyelinating disorder of peripheral nerves."
    Okuda T., Higashi Y., Kokame K., Tanaka C., Kondoh H., Miyata T.
    Mol. Cell. Biol. 24:3949-3956(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330 AND SER-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-319; SER-326; THR-328; SER-330; SER-332; SER-333; THR-335; SER-336; THR-340 AND SER-342, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  9. "NDRG4 protein-deficient mice exhibit spatial learning deficits and vulnerabilities to cerebral ischemia."
    Yamamoto H., Kokame K., Okuda T., Nakajo Y., Yanamoto H., Miyata T.
    J. Biol. Chem. 286:26158-26165(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  10. Cited for: DISRUPTION PHENOTYPE, CHARACTERISTICS OF A MOUSE MODEL OF CMT4D, FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNDRG1_MOUSE
AccessioniPrimary (citable) accession number: Q62433
Secondary accession number(s): P97862
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.