Protein NDRG1 - Mus musculus (Mouse)

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Q62433 (NDRG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein NDRG1

Alternative name(s):
N-myc downstream-regulated gene 1 protein
Short name=Protein Ndr1

Gene names

Name:	Ndrg1
Synonyms:	Ndr1, Ndrl, Tdd5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy By similarity. Has a role in cell trafficking notably of the Schwann cell and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Ref.5 Ref.9
Subunit structure	Interacts with RAB4A (membrane-bound form); the interaction involves NDRG1 in vesicular recycling of CDH1. Interacts with APOA1, APOA2, PRA1 and RTN1 By similarity.
Subcellular location	Cytoplasm › cytosol. Cytoplasm › cytoskeleton › centrosome. Nucleus. Cell membrane By similarity. Note: Mainly cytoplasmic but differentially localized to other regions. Associates with the plasma membrane in intestinal epithelia and lactating mammary gland. Translocated to the nucleus in a p53/TP53-dependent manner. In prostate epithelium and placental chorion, located in both the cytoplasm and in the nucleus. No nuclear localization in colon epithelium cells. In intestinal mucosa, prostate and renal cortex, located predominantly adjacent to adherens junctions. Cytoplasmic with granular staining in proximal tubular cells of the kidney and salivary gland ducts. Recruits to the membrane of recycling/sorting and late endosomes via binding to phosphatidylinositol 4-phosphate. Associates with microtubules. Colocalizes with TUBG1 in the centrosome. Cytoplasmic location increased with hypoxia. Phosphorylated form found associated with centromeres during S-phase of mitosis and with the plasma membrane By similarity. Ref.1 Ref.4 Ref.5 Ref.9
Tissue specificity	Widely expressed, with highest levels in kidney followed by brain, pancreas, small intestine, colon and spleen (at protein level). Also detected in heart and preputial gland, and in much smaller quantities in other tissues. Not detected in duodenum and prostate. Highly expressed in Schwann cells. Ref.2 Ref.4 Ref.5 Ref.8
Developmental stage	In early stages of embryo development, expression low when MYCN expression is high. Later, when MYCN levels diminish, levels increase. Ref.1
Induction	Repressed by testosterone and also to a lesser extent by dihydrotestosterone. Down-regulated by MYCN. Ref.1 Ref.2
Post-translational modification	Under stress conditions, phosphorylated in the C-terminal on many serine and threonine residues. Phosphorylated in vitro by PKA. Phosphorylation enhanced by increased intracellular cAMP levels. Homocysteine induces dephosphorylation. Phosphorylation by SGK1 is cell cycle dependent By similarity. Ref.4
Disruption phenotype	Mutant mice exhibit defects in peripheral nerve development. Initial hind limb weakness developed around age 12 weeks, and significant functional impairment (dragging of hind legs) and muscle atrophy became apparent at age 1 year. After about 5 weeks extensive demyelination of nerve fibers is observed. In later life, large inclusions were seen in the adaxonal Schwann cell cytoplasm. There is no evidence of apoptotic response. Ref.5 Ref.9
Sequence similarities	Belongs to the NDRG family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane Microtubule Nucleus
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage response, signal transduction by p53 class mediator Inferred from electronic annotation. Source: Compara cellular response to hypoxia Inferred from electronic annotation. Source: Compara mast cell activation Inferred from direct assay PubMed 12804568. Source: MGI peripheral nervous system myelin maintenance Inferred from mutant phenotype Ref.5. Source: UniProtKB positive regulation of spindle checkpoint Inferred from electronic annotation. Source: Compara
Cellular_component	cell-cell adherens junction Inferred from electronic annotation. Source: Compara centrosome Inferred from electronic annotation. Source: Compara cytoplasm Inferred from direct assay Ref.5. Source: UniProtKB cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell recycling endosome membrane Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 394

394

Protein NDRG1

PRO_0000159574

Regions

Repeat

Repeat

Repeat

Region

3 X 10 AA tandem repeats of G-[PST]-R-S-R-S-H-T-S-E

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Modified residue

326

Phosphoserine By similarity

Modified residue

328

Phosphothreonine; by SGK1 Ref.6

Modified residue

330

Phosphoserine; by SGK1 Ref.6

Modified residue

332

Phosphoserine; by SGK1 By similarity

Modified residue

333

Phosphoserine By similarity

Modified residue

335

Phosphothreonine Ref.6

Modified residue

336

Phosphoserine Ref.6

Modified residue

346

Phosphothreonine; by SGK1 Ref.4 Ref.7

Modified residue

356

Phosphothreonine; by SGK1 By similarity

Modified residue

362

Phosphoserine Ref.7

Modified residue

364

Phosphoserine By similarity

Modified residue

366

Phosphothreonine; by SGK1 By similarity

Modified residue

375

Phosphothreonine By similarity

Experimental info

Sequence conflict

33 – 35

QEQ → LEE in AAB58249. Ref.2

Sequence conflict

F → P in AAB58249. Ref.2

Sequence conflict

103 – 109

APSFPVG → PLPSQW in AAB58249. Ref.2

Sequence conflict

141 – 148

AGAYILTR → PWXLHPDP in AAB58249. Ref.2

Sequence conflict

191 – 208

VVSHL…NVEVV → CVPPLRXGGDTQQRGGM in AAB58249. Ref.2

Sequence conflict

241

R → A in AAB58249. Ref.2

Sequence conflict

298 – 350

PAKLA…TSEGP → RPSLLRPSSTLCRHGIHAFC QHDSPDRVPHPVWLQCHILE GT in AAB58249. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q62433 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 905CA71ECF4C87C2
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FASTA

394

43,009

        10         20         30         40         50         60 
MSRELHDVDL AEVKPLVEKG ESITGLLQEF DVQEQDIETL HGSLHVTLCG TPKGNRPVIL 

        70         80         90        100        110        120 
TYHDIGMNHK TCYNPLFNSE DMQEITQHFA VCHVDAPGQQ DGAPSFPVGY MYPSMDQLAE 

       130        140        150        160        170        180 
MLPGVLHQFG LKSVIGMGTG AGAYILTRFA LNNPEMVEGL VLMNVNPCAE GWMDWAASKI 

       190        200        210        220        230        240 
SGWTQALPDM VVSHLFGKEE IHNNVEVVHT YRQHILNDMN PSNLHLFISA YNSRRDLEIE 

       250        260        270        280        290        300 
RPMPGTHTVT LQCPALLVVG DNSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK 

       310        320        330        340        350        360 
LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLEGT RSRSHTSEGP RSRSHTSEGS 

       370        380        390 
RSRSHTSEDA RLNITPNSGA TGNNAGPKSM EVSC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"N-myc-dependent repression of ndr1, a gene identified by direct subtraction of whole mouse embryo cDNAs between wild type and N-myc mutant." Shimono A., Okuda T., Kondoh H. Mech. Dev. 83:39-52(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION. Tissue: Embryo.
[2]	"Cloning and characterization of TDD5, an androgen target gene that is differentially repressed by testosterone and dihydrotestosterone." Lin T.-M., Chang C. Proc. Natl. Acad. Sci. U.S.A. 94:4988-4993(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Tissue: Hybridoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[4]	"Exploitation of KESTREL to identify NDRG family members as physiological substrates for SGK1 and GSK3." Murray J.T., Campbell D.G., Morrice N., Auld G.C., Shpiro N., Marquez R., Peggie M., Bain J., Bloomberg G.B., Grahammer F., Lang F., Wulff P., Kuhl D., Cohen P. Biochem. J. 384:477-488(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT THR-346; THR-356 AND THR-366, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
[5]	"Ndrg1-deficient mice exhibit a progressive demyelinating disorder of peripheral nerves." Okuda T., Higashi Y., Kokame K., Tanaka C., Kondoh H., Miyata T. Mol. Cell. Biol. 24:3949-3956(2004) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, FUNCTION.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-328; SER-330; THR-335 AND SER-336, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; THR-346 AND SER-362, MASS SPECTROMETRY. Tissue: Melanoma.
[8]	"NDRG4 protein-deficient mice exhibit spatial learning deficits and vulnerabilities to cerebral ischemia." Yamamoto H., Kokame K., Okuda T., Nakajo Y., Yanamoto H., Miyata T. J. Biol. Chem. 286:26158-26165(2011) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"Ndrg1 in development and maintenance of the myelin sheath." King R.H., Chandler D., Lopaticki S., Huang D., Blake J., Muddle J.R., Kilpatrick T., Nourallah M., Miyata T., Okuda T., Carter K.W., Hunter M., Angelicheva D., Morahan G., Kalaydjieva L. Neurobiol. Dis. 42:368-380(2011) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, CHARACTERISTICS OF A MOUSE MODEL OF CMT4D, FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U60593 mRNA. Translation: AAB03484.1. U52073 mRNA. Translation: AAB58249.1. BC015282 mRNA. Translation: AAH15282.1. BC071235 mRNA. Translation: AAH71235.1.
IPI	IPI00125960.
RefSeq	NP_032707.2. NM_008681.2.
UniGene	Mm.30837.
3D structure databases
ProteinModelPortal	Q62433.
SMR	Q62433. Positions 33-315.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62433. 1 interaction.
Protein family/group databases
MEROPS	S33.987.
PTM databases
PhosphoSite	Q62433.
Proteomic databases
PaxDb	Q62433.
PRIDE	Q62433.
Protocols and materials databases
DNASU	17988.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000005256; ENSMUSP00000005256; ENSMUSG00000005125.
GeneID	17988.
KEGG	mmu:17988.
Organism-specific databases
CTD	10397.
MGI	MGI:1341799. Ndrg1.
Phylogenomic databases
eggNOG	NOG310435.
GeneTree	ENSGT00390000001874.
HOGENOM	HOG000230891.
HOVERGEN	HBG052591.
InParanoid	Q62433.
OMA	LHGSIHV.
OrthoDB	EOG4QC15K.
Gene expression databases
ArrayExpress	Q62433.
Bgee	Q62433.
CleanEx	MM_NDRG1.
Genevestigator	Q62433.
GermOnline	ENSMUSG00000005125. Mus musculus.
Family and domain databases
InterPro	IPR004142. Ndr. [Graphical view]
PANTHER	PTHR11034. PTHR11034. 1 hit.
Pfam	PF03096. Ndr. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NDRG1. mouse.
NextBio	292963.
SOURCE	Search...

Entry information

Entry name

NDRG1_MOUSE

Accession

Primary (citable) accession number: Q62433
Secondary accession number(s): P97862

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents