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Q62431

- ARI3A_MOUSE

UniProt

Q62431 - ARI3A_MOUSE

Protein

AT-rich interactive domain-containing protein 3A

Gene

Arid3a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Transcription factor involved in B-cell differentiation. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. May be involved in the control of cell cycle progression by the RB1/E2F1 pathway.3 Publications

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. protein binding Source: MGI
    4. protein homodimerization activity Source: MGI
    5. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
    6. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    7. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    2. regulation of transcription, DNA-templated Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AT-rich interactive domain-containing protein 3A
    Short name:
    ARID domain-containing protein 3A
    Alternative name(s):
    B-cell regulator of IgH transcription
    Short name:
    Bright
    Dead ringer-like protein 1
    Gene namesi
    Name:Arid3a
    Synonyms:Dri1, Dril1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1328360. Arid3a.

    Subcellular locationi

    Nucleus. Cytoplasm
    Note: Shuttles between nucleus and cytoplasm.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane raft Source: Ensembl
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi268 – 2681P → A: Impairs DNA-binding. 1 Publication
    Mutagenesisi299 – 2991W → A: Impairs DNA-binding. 1 Publication
    Mutagenesisi317 – 3171F → A: Impairs DNA-binding. 1 Publication
    Mutagenesisi330 – 3301Y → A: Impairs DNA-binding. 1 Publication
    Mutagenesisi457 – 4571K → A: No effect on cellular location. 1 Publication
    Mutagenesisi463 – 4631P → A: Abolishes nuclear localization. 1 Publication
    Mutagenesisi466 – 4661K → A: Abolishes nuclear localization. 1 Publication
    Mutagenesisi467 – 4671K → A: Abolishes nuclear localization. 1 Publication
    Mutagenesisi532 – 5321G → A: Abolishes cytosolic localization. 1 Publication
    Mutagenesisi535 – 5351Y → A: Abolishes cytosolic localization. 1 Publication
    Mutagenesisi535 – 5351Y → F: No effect on cellular location. 1 Publication
    Mutagenesisi537 – 5371G → A: Abolishes cytosolic localization. 1 Publication
    Mutagenesisi539 – 5391L → A: Abolishes cytosolic localization. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 601601AT-rich interactive domain-containing protein 3APRO_0000200579Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei78 – 781PhosphoserineBy similarity
    Modified residuei82 – 821PhosphoserineBy similarity
    Modified residuei89 – 891PhosphoserineBy similarity
    Modified residuei99 – 991PhosphothreonineBy similarity
    Modified residuei102 – 1021PhosphoserineBy similarity
    Modified residuei127 – 1271Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ62431.
    PRIDEiQ62431.

    PTM databases

    PhosphoSiteiQ62431.

    Expressioni

    Tissue specificityi

    B-cell specific in the adult. Expressed in B-cell progenitors, down-regulated in the immature B-cell stage, and is up-regulated again at later stages of B-lymphocyte differentiation.1 Publication

    Developmental stagei

    Expressed in lymphocytes from fetal liver. Expressed in fetal thymus and brain.1 Publication

    Gene expression databases

    ArrayExpressiQ62431.
    BgeeiQ62431.
    CleanExiMM_ARID3A.
    GenevestigatoriQ62431.

    Interactioni

    Subunit structurei

    Homodimer. Heterodimer with ARID3B. Interacts with E2F1 By similarity. Interacts with GTF2I and BTK.By similarity3 Publications

    Protein-protein interaction databases

    BioGridi199312. 2 interactions.
    IntActiQ62431. 1 interaction.
    MINTiMINT-4088325.
    STRINGi10090.ENSMUSP00000019708.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62431.
    SMRiQ62431. Positions 223-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini243 – 33593ARIDPROSITE-ProRule annotationAdd
    BLAST
    Domaini449 – 54698REKLESPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni128 – 16538AcidicAdd
    BLAST
    Regioni450 – 49344Important for nuclear localizationAdd
    BLAST
    Regioni495 – 51824HomodimerizationBy similarityAdd
    BLAST
    Regioni542 – 56221Important for cytoplasmic localizationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi27 – 348Poly-Pro
    Compositional biasi68 – 714Poly-Ala
    Compositional biasi128 – 1314Poly-Asp
    Compositional biasi146 – 1549Poly-Glu
    Compositional biasi158 – 1658Poly-Glu
    Compositional biasi437 – 44610Poly-Ala
    Compositional biasi543 – 5464Poly-Pro

    Sequence similaritiesi

    Contains 1 ARID domain.PROSITE-ProRule annotation
    Contains 1 REKLES domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG327790.
    GeneTreeiENSGT00550000074575.
    HOGENOMiHOG000236281.
    HOVERGENiHBG050574.
    InParanoidiQ62431.
    OMAiLFAYSPG.
    OrthoDBiEOG77DJ6W.
    PhylomeDBiQ62431.
    TreeFamiTF320364.

    Family and domain databases

    Gene3Di1.10.150.60. 1 hit.
    InterProiIPR001606. ARID/BRIGHT_DNA-bd.
    IPR023334. REKLES_domain.
    [Graphical view]
    PfamiPF01388. ARID. 1 hit.
    [Graphical view]
    SMARTiSM00501. BRIGHT. 1 hit.
    [Graphical view]
    SUPFAMiSSF46774. SSF46774. 1 hit.
    PROSITEiPS51011. ARID. 1 hit.
    PS51486. REKLES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q62431-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLQAVMETL IQRQQRARQE LEARQAPPPP PPEPTGVRAR TTMTDEDREP    50
    ENARMHRTQM AALAAMRAAA AGLGHPSSPG GSEDGPPISG DEDTAREGTL 100
    SSPALHGSVL EGAGHAEGDR HLMDVGSDDD DTKSKWEEQE LEELGEEEEE 150
    EEEEDDFEEE EEEEEGLGPP ESASLGTAGL FTRKAPPAQA FRGDGGPRML 200
    SGPERLGPGP AHPSHMASQM PPPDHGDWTF EEQFKQLYEL DADPKRKEFL 250
    DDLFSFMQKR GTPVNRIPIM AKQVLDLFML YVLVTEKGGL VEVINKKLWR 300
    EITKGLNLPT SITSAAFTLR TQYMKYLYPY ECERRGLSSP NELQAAIDSN 350
    RREGRRQSFG GSLFAYSPSG AHSMLPSPKL PVTPLGLAAS TNGSSITPAP 400
    KIKKEEDSAI PITVPGRLPV SLAGHPVVAA QAAAVQAAAA QAAVAAQAAA 450
    LEQLREKLES TEPPEKKMAL VADEQQRLMQ RAVQQSFLAM TAQLPMNIRI 500
    NSQASESRQD SAVSLTSANG SNSISMSVEM NGIVYTGVLF AQPPPPTAPS 550
    APGKGGVSSI GTNTTTGSRT GASGSTVSGG QVGLPGVSTP TMSSTSNNSL 600
    P 601
    Length:601
    Mass (Da):64,173
    Last modified:November 1, 1996 - v1
    Checksum:i66994C01E1FB68CD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti405 – 4062Missing in AAH50925. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60335 mRNA. Translation: AAB03416.1.
    AK141283 mRNA. Translation: BAE24635.1.
    AK154956 mRNA. Translation: BAE32951.1.
    BC050925 mRNA. Translation: AAH50925.1.
    CCDSiCCDS23999.1.
    RefSeqiNP_001275554.1. NM_001288625.1.
    NP_001275555.1. NM_001288626.1.
    NP_031906.1. NM_007880.4.
    XP_006513264.1. XM_006513201.1.
    XP_006513265.1. XM_006513202.1.
    UniGeneiMm.301282.

    Genome annotation databases

    EnsembliENSMUST00000019708; ENSMUSP00000019708; ENSMUSG00000019564.
    ENSMUST00000105376; ENSMUSP00000101015; ENSMUSG00000019564.
    ENSMUST00000105377; ENSMUSP00000101016; ENSMUSG00000019564.
    GeneIDi13496.
    KEGGimmu:13496.
    UCSCiuc007gap.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60335 mRNA. Translation: AAB03416.1 .
    AK141283 mRNA. Translation: BAE24635.1 .
    AK154956 mRNA. Translation: BAE32951.1 .
    BC050925 mRNA. Translation: AAH50925.1 .
    CCDSi CCDS23999.1.
    RefSeqi NP_001275554.1. NM_001288625.1.
    NP_001275555.1. NM_001288626.1.
    NP_031906.1. NM_007880.4.
    XP_006513264.1. XM_006513201.1.
    XP_006513265.1. XM_006513202.1.
    UniGenei Mm.301282.

    3D structure databases

    ProteinModelPortali Q62431.
    SMRi Q62431. Positions 223-356.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199312. 2 interactions.
    IntActi Q62431. 1 interaction.
    MINTi MINT-4088325.
    STRINGi 10090.ENSMUSP00000019708.

    PTM databases

    PhosphoSitei Q62431.

    Proteomic databases

    PaxDbi Q62431.
    PRIDEi Q62431.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019708 ; ENSMUSP00000019708 ; ENSMUSG00000019564 .
    ENSMUST00000105376 ; ENSMUSP00000101015 ; ENSMUSG00000019564 .
    ENSMUST00000105377 ; ENSMUSP00000101016 ; ENSMUSG00000019564 .
    GeneIDi 13496.
    KEGGi mmu:13496.
    UCSCi uc007gap.1. mouse.

    Organism-specific databases

    CTDi 1820.
    MGIi MGI:1328360. Arid3a.

    Phylogenomic databases

    eggNOGi NOG327790.
    GeneTreei ENSGT00550000074575.
    HOGENOMi HOG000236281.
    HOVERGENi HBG050574.
    InParanoidi Q62431.
    OMAi LFAYSPG.
    OrthoDBi EOG77DJ6W.
    PhylomeDBi Q62431.
    TreeFami TF320364.

    Miscellaneous databases

    ChiTaRSi ARID3A. mouse.
    NextBioi 284025.
    PROi Q62431.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q62431.
    Bgeei Q62431.
    CleanExi MM_ARID3A.
    Genevestigatori Q62431.

    Family and domain databases

    Gene3Di 1.10.150.60. 1 hit.
    InterProi IPR001606. ARID/BRIGHT_DNA-bd.
    IPR023334. REKLES_domain.
    [Graphical view ]
    Pfami PF01388. ARID. 1 hit.
    [Graphical view ]
    SMARTi SM00501. BRIGHT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46774. SSF46774. 1 hit.
    PROSITEi PS51011. ARID. 1 hit.
    PS51486. REKLES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The immunoglobulin heavy-chain matrix-associating regions are bound by Bright: a B cell-specific trans-activator that describes a new DNA-binding protein family."
      Herrscher R.F., Kaplan M.H., Lelsz D.L., Das C., Scheuermann R., Tucker P.W.
      Genes Dev. 9:3067-3082(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Expression of bright at two distinct stages of B lymphocyte development."
      Webb C.F., Smith E.A., Medina K.L., Buchanan K.L., Smithson G., Dou S.
      J. Immunol. 160:4747-4754(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    5. "The transcription factor Bright associates with Bruton's tyrosine kinase, the defective protein in immunodeficiency disease."
      Webb C.F., Yamashita Y., Ayers N., Evetts S., Paulin Y., Conley M.E., Smith E.A.
      J. Immunol. 165:6956-6965(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BTK.
    6. "Transcriptional activation by a matrix associating region-binding protein. contextual requirements for the function of bright."
      Kaplan M.H., Zong R.-T., Herrscher R.F., Scheuermann R.H., Tucker P.W.
      J. Biol. Chem. 276:21325-21330(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Mutations in the DNA-binding domain of the transcription factor Bright act as dominant negative proteins and interfere with immunoglobulin transactivation."
      Nixon J.C., Rajaiya J., Webb C.F.
      J. Biol. Chem. 279:52465-52472(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF PRO-268; TRP-299; PHE-317 AND TYR-330.
    8. "A regulated nucleocytoplasmic shuttle contributes to Bright's function as a transcriptional activator of immunoglobulin genes."
      Kim D., Tucker P.W.
      Mol. Cell. Biol. 26:2187-2201(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-457; PRO-463; LYS-466; LYS-467; GLY-532; TYR-535; GLY-537 AND LEU-539.
    9. "Induction of immunoglobulin heavy-chain transcription through the transcription factor Bright requires TFII-I."
      Rajaiya J., Nixon J.C., Ayers N., Desgranges Z.P., Roy A.L., Webb C.F.
      Mol. Cell. Biol. 26:4758-4768(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GTF2I AND BTK.
    10. "Anti-nuclear antibody production and autoimmunity in transgenic mice that overexpress the transcription factor Bright."
      Shankar M., Nixon J.C., Maier S., Workman J., Farris A.D., Webb C.F.
      J. Immunol. 178:2996-3006(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-127, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiARI3A_MOUSE
    AccessioniPrimary (citable) accession number: Q62431
    Secondary accession number(s): Q3U338, Q80YP8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3