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Q62431

- ARI3A_MOUSE

UniProt

Q62431 - ARI3A_MOUSE

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Protein

AT-rich interactive domain-containing protein 3A

Gene

Arid3a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor involved in B-cell differentiation. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. May be involved in the control of cell cycle progression by the RB1/E2F1 pathway.3 Publications

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: MGI
  3. protein homodimerization activity Source: MGI
  4. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  5. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  6. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  2. regulation of transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
AT-rich interactive domain-containing protein 3A
Short name:
ARID domain-containing protein 3A
Alternative name(s):
B-cell regulator of IgH transcription
Short name:
Bright
Dead ringer-like protein 1
Gene namesi
Name:Arid3a
Synonyms:Dri1, Dril1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1328360. Arid3a.

Subcellular locationi

Nucleus. Cytoplasm
Note: Shuttles between nucleus and cytoplasm.

GO - Cellular componenti

  1. Golgi apparatus Source: Ensembl
  2. membrane raft Source: Ensembl
  3. nucleolus Source: Ensembl
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi268 – 2681P → A: Impairs DNA-binding. 1 Publication
Mutagenesisi299 – 2991W → A: Impairs DNA-binding. 1 Publication
Mutagenesisi317 – 3171F → A: Impairs DNA-binding. 1 Publication
Mutagenesisi330 – 3301Y → A: Impairs DNA-binding. 1 Publication
Mutagenesisi457 – 4571K → A: No effect on cellular location. 1 Publication
Mutagenesisi463 – 4631P → A: Abolishes nuclear localization. 1 Publication
Mutagenesisi466 – 4661K → A: Abolishes nuclear localization. 1 Publication
Mutagenesisi467 – 4671K → A: Abolishes nuclear localization. 1 Publication
Mutagenesisi532 – 5321G → A: Abolishes cytosolic localization. 1 Publication
Mutagenesisi535 – 5351Y → A: Abolishes cytosolic localization. 1 Publication
Mutagenesisi535 – 5351Y → F: No effect on cellular location. 1 Publication
Mutagenesisi537 – 5371G → A: Abolishes cytosolic localization. 1 Publication
Mutagenesisi539 – 5391L → A: Abolishes cytosolic localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601AT-rich interactive domain-containing protein 3APRO_0000200579Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781PhosphoserineBy similarity
Modified residuei82 – 821PhosphoserineBy similarity
Modified residuei89 – 891PhosphoserineBy similarity
Modified residuei99 – 991PhosphothreonineBy similarity
Modified residuei102 – 1021PhosphoserineBy similarity
Modified residuei127 – 1271Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ62431.
PaxDbiQ62431.
PRIDEiQ62431.

PTM databases

PhosphoSiteiQ62431.

Expressioni

Tissue specificityi

B-cell specific in the adult. Expressed in B-cell progenitors, down-regulated in the immature B-cell stage, and is up-regulated again at later stages of B-lymphocyte differentiation.1 Publication

Developmental stagei

Expressed in lymphocytes from fetal liver. Expressed in fetal thymus and brain.1 Publication

Gene expression databases

BgeeiQ62431.
CleanExiMM_ARID3A.
ExpressionAtlasiQ62431. baseline.
GenevestigatoriQ62431.

Interactioni

Subunit structurei

Homodimer. Heterodimer with ARID3B. Interacts with E2F1 (By similarity). Interacts with GTF2I and BTK.By similarity3 Publications

Protein-protein interaction databases

BioGridi199312. 2 interactions.
IntActiQ62431. 1 interaction.
MINTiMINT-4088325.
STRINGi10090.ENSMUSP00000019708.

Structurei

3D structure databases

ProteinModelPortaliQ62431.
SMRiQ62431. Positions 223-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini243 – 33593ARIDPROSITE-ProRule annotationAdd
BLAST
Domaini449 – 54698REKLESPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni128 – 16538AcidicAdd
BLAST
Regioni450 – 49344Important for nuclear localizationAdd
BLAST
Regioni495 – 51824HomodimerizationBy similarityAdd
BLAST
Regioni542 – 56221Important for cytoplasmic localizationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi27 – 348Poly-Pro
Compositional biasi68 – 714Poly-Ala
Compositional biasi128 – 1314Poly-Asp
Compositional biasi146 – 1549Poly-Glu
Compositional biasi158 – 1658Poly-Glu
Compositional biasi437 – 44610Poly-Ala
Compositional biasi543 – 5464Poly-Pro

Sequence similaritiesi

Contains 1 ARID domain.PROSITE-ProRule annotation
Contains 1 REKLES domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG327790.
GeneTreeiENSGT00550000074575.
HOGENOMiHOG000236281.
HOVERGENiHBG050574.
InParanoidiQ62431.
OMAiLFAYSPG.
OrthoDBiEOG77DJ6W.
PhylomeDBiQ62431.
TreeFamiTF320364.

Family and domain databases

Gene3Di1.10.150.60. 1 hit.
InterProiIPR001606. ARID/BRIGHT_DNA-bd.
IPR023334. REKLES_domain.
[Graphical view]
PfamiPF01388. ARID. 1 hit.
[Graphical view]
SMARTiSM00501. BRIGHT. 1 hit.
[Graphical view]
SUPFAMiSSF46774. SSF46774. 1 hit.
PROSITEiPS51011. ARID. 1 hit.
PS51486. REKLES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62431-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLQAVMETL IQRQQRARQE LEARQAPPPP PPEPTGVRAR TTMTDEDREP
60 70 80 90 100
ENARMHRTQM AALAAMRAAA AGLGHPSSPG GSEDGPPISG DEDTAREGTL
110 120 130 140 150
SSPALHGSVL EGAGHAEGDR HLMDVGSDDD DTKSKWEEQE LEELGEEEEE
160 170 180 190 200
EEEEDDFEEE EEEEEGLGPP ESASLGTAGL FTRKAPPAQA FRGDGGPRML
210 220 230 240 250
SGPERLGPGP AHPSHMASQM PPPDHGDWTF EEQFKQLYEL DADPKRKEFL
260 270 280 290 300
DDLFSFMQKR GTPVNRIPIM AKQVLDLFML YVLVTEKGGL VEVINKKLWR
310 320 330 340 350
EITKGLNLPT SITSAAFTLR TQYMKYLYPY ECERRGLSSP NELQAAIDSN
360 370 380 390 400
RREGRRQSFG GSLFAYSPSG AHSMLPSPKL PVTPLGLAAS TNGSSITPAP
410 420 430 440 450
KIKKEEDSAI PITVPGRLPV SLAGHPVVAA QAAAVQAAAA QAAVAAQAAA
460 470 480 490 500
LEQLREKLES TEPPEKKMAL VADEQQRLMQ RAVQQSFLAM TAQLPMNIRI
510 520 530 540 550
NSQASESRQD SAVSLTSANG SNSISMSVEM NGIVYTGVLF AQPPPPTAPS
560 570 580 590 600
APGKGGVSSI GTNTTTGSRT GASGSTVSGG QVGLPGVSTP TMSSTSNNSL

P
Length:601
Mass (Da):64,173
Last modified:November 1, 1996 - v1
Checksum:i66994C01E1FB68CD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti405 – 4062Missing in AAH50925. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60335 mRNA. Translation: AAB03416.1.
AK141283 mRNA. Translation: BAE24635.1.
AK154956 mRNA. Translation: BAE32951.1.
BC050925 mRNA. Translation: AAH50925.1.
CCDSiCCDS23999.1.
RefSeqiNP_001275554.1. NM_001288625.1.
NP_001275555.1. NM_001288626.1.
NP_031906.1. NM_007880.4.
XP_006513264.1. XM_006513201.1.
XP_006513265.1. XM_006513202.1.
UniGeneiMm.301282.

Genome annotation databases

EnsembliENSMUST00000019708; ENSMUSP00000019708; ENSMUSG00000019564.
ENSMUST00000105376; ENSMUSP00000101015; ENSMUSG00000019564.
ENSMUST00000105377; ENSMUSP00000101016; ENSMUSG00000019564.
GeneIDi13496.
KEGGimmu:13496.
UCSCiuc007gap.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60335 mRNA. Translation: AAB03416.1 .
AK141283 mRNA. Translation: BAE24635.1 .
AK154956 mRNA. Translation: BAE32951.1 .
BC050925 mRNA. Translation: AAH50925.1 .
CCDSi CCDS23999.1.
RefSeqi NP_001275554.1. NM_001288625.1.
NP_001275555.1. NM_001288626.1.
NP_031906.1. NM_007880.4.
XP_006513264.1. XM_006513201.1.
XP_006513265.1. XM_006513202.1.
UniGenei Mm.301282.

3D structure databases

ProteinModelPortali Q62431.
SMRi Q62431. Positions 223-356.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 199312. 2 interactions.
IntActi Q62431. 1 interaction.
MINTi MINT-4088325.
STRINGi 10090.ENSMUSP00000019708.

PTM databases

PhosphoSitei Q62431.

Proteomic databases

MaxQBi Q62431.
PaxDbi Q62431.
PRIDEi Q62431.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000019708 ; ENSMUSP00000019708 ; ENSMUSG00000019564 .
ENSMUST00000105376 ; ENSMUSP00000101015 ; ENSMUSG00000019564 .
ENSMUST00000105377 ; ENSMUSP00000101016 ; ENSMUSG00000019564 .
GeneIDi 13496.
KEGGi mmu:13496.
UCSCi uc007gap.1. mouse.

Organism-specific databases

CTDi 1820.
MGIi MGI:1328360. Arid3a.

Phylogenomic databases

eggNOGi NOG327790.
GeneTreei ENSGT00550000074575.
HOGENOMi HOG000236281.
HOVERGENi HBG050574.
InParanoidi Q62431.
OMAi LFAYSPG.
OrthoDBi EOG77DJ6W.
PhylomeDBi Q62431.
TreeFami TF320364.

Miscellaneous databases

ChiTaRSi Arid3a. mouse.
NextBioi 284025.
PROi Q62431.
SOURCEi Search...

Gene expression databases

Bgeei Q62431.
CleanExi MM_ARID3A.
ExpressionAtlasi Q62431. baseline.
Genevestigatori Q62431.

Family and domain databases

Gene3Di 1.10.150.60. 1 hit.
InterProi IPR001606. ARID/BRIGHT_DNA-bd.
IPR023334. REKLES_domain.
[Graphical view ]
Pfami PF01388. ARID. 1 hit.
[Graphical view ]
SMARTi SM00501. BRIGHT. 1 hit.
[Graphical view ]
SUPFAMi SSF46774. SSF46774. 1 hit.
PROSITEi PS51011. ARID. 1 hit.
PS51486. REKLES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The immunoglobulin heavy-chain matrix-associating regions are bound by Bright: a B cell-specific trans-activator that describes a new DNA-binding protein family."
    Herrscher R.F., Kaplan M.H., Lelsz D.L., Das C., Scheuermann R., Tucker P.W.
    Genes Dev. 9:3067-3082(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Expression of bright at two distinct stages of B lymphocyte development."
    Webb C.F., Smith E.A., Medina K.L., Buchanan K.L., Smithson G., Dou S.
    J. Immunol. 160:4747-4754(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "The transcription factor Bright associates with Bruton's tyrosine kinase, the defective protein in immunodeficiency disease."
    Webb C.F., Yamashita Y., Ayers N., Evetts S., Paulin Y., Conley M.E., Smith E.A.
    J. Immunol. 165:6956-6965(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BTK.
  6. "Transcriptional activation by a matrix associating region-binding protein. contextual requirements for the function of bright."
    Kaplan M.H., Zong R.-T., Herrscher R.F., Scheuermann R.H., Tucker P.W.
    J. Biol. Chem. 276:21325-21330(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Mutations in the DNA-binding domain of the transcription factor Bright act as dominant negative proteins and interfere with immunoglobulin transactivation."
    Nixon J.C., Rajaiya J., Webb C.F.
    J. Biol. Chem. 279:52465-52472(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF PRO-268; TRP-299; PHE-317 AND TYR-330.
  8. "A regulated nucleocytoplasmic shuttle contributes to Bright's function as a transcriptional activator of immunoglobulin genes."
    Kim D., Tucker P.W.
    Mol. Cell. Biol. 26:2187-2201(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-457; PRO-463; LYS-466; LYS-467; GLY-532; TYR-535; GLY-537 AND LEU-539.
  9. "Induction of immunoglobulin heavy-chain transcription through the transcription factor Bright requires TFII-I."
    Rajaiya J., Nixon J.C., Ayers N., Desgranges Z.P., Roy A.L., Webb C.F.
    Mol. Cell. Biol. 26:4758-4768(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GTF2I AND BTK.
  10. "Anti-nuclear antibody production and autoimmunity in transgenic mice that overexpress the transcription factor Bright."
    Shankar M., Nixon J.C., Maier S., Workman J., Farris A.D., Webb C.F.
    J. Immunol. 178:2996-3006(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-127, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiARI3A_MOUSE
AccessioniPrimary (citable) accession number: Q62431
Secondary accession number(s): Q3U338, Q80YP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3