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Protein

AT-rich interactive domain-containing protein 3A

Gene

Arid3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in B-cell differentiation. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. May be involved in the control of cell cycle progression by the RB1/E2F1 pathway.3 Publications

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: MGI
  • protein homodimerization activity Source: MGI
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: MGI

GO - Biological processi

  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
AT-rich interactive domain-containing protein 3A
Short name:
ARID domain-containing protein 3A
Alternative name(s):
B-cell regulator of IgH transcription
Short name:
Bright
Dead ringer-like protein 1
Gene namesi
Name:Arid3a
Synonyms:Dri1, Dril1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1328360. Arid3a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • Golgi apparatus Source: MGI
  • membrane raft Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi268P → A: Impairs DNA-binding. 1 Publication1
Mutagenesisi299W → A: Impairs DNA-binding. 1 Publication1
Mutagenesisi317F → A: Impairs DNA-binding. 1 Publication1
Mutagenesisi330Y → A: Impairs DNA-binding. 1 Publication1
Mutagenesisi457K → A: No effect on cellular location. 1 Publication1
Mutagenesisi463P → A: Abolishes nuclear localization. 1 Publication1
Mutagenesisi466K → A: Abolishes nuclear localization. 1 Publication1
Mutagenesisi467K → A: Abolishes nuclear localization. 1 Publication1
Mutagenesisi532G → A: Abolishes cytosolic localization. 1 Publication1
Mutagenesisi535Y → A: Abolishes cytosolic localization. 1 Publication1
Mutagenesisi535Y → F: No effect on cellular location. 1 Publication1
Mutagenesisi537G → A: Abolishes cytosolic localization. 1 Publication1
Mutagenesisi539L → A: Abolishes cytosolic localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005791 – 601AT-rich interactive domain-containing protein 3AAdd BLAST601

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78PhosphoserineBy similarity1
Modified residuei82PhosphoserineCombined sources1
Modified residuei89PhosphoserineCombined sources1
Modified residuei99PhosphothreonineBy similarity1
Modified residuei102PhosphoserineBy similarity1
Modified residuei127PhosphoserineCombined sources1
Modified residuei358PhosphoserineCombined sources1
Modified residuei367PhosphoserineBy similarity1
Cross-linki457Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ62431.
MaxQBiQ62431.
PaxDbiQ62431.
PeptideAtlasiQ62431.
PRIDEiQ62431.

PTM databases

iPTMnetiQ62431.
PhosphoSitePlusiQ62431.

Expressioni

Tissue specificityi

B-cell specific in the adult. Expressed in B-cell progenitors, down-regulated in the immature B-cell stage, and is up-regulated again at later stages of B-lymphocyte differentiation.1 Publication

Developmental stagei

Expressed in lymphocytes from fetal liver. Expressed in fetal thymus and brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000019564.
CleanExiMM_ARID3A.
ExpressionAtlasiQ62431. baseline and differential.
GenevisibleiQ62431. MM.

Interactioni

Subunit structurei

Homodimer. Heterodimer with ARID3B. Interacts with E2F1 (By similarity). Interacts with GTF2I and BTK.By similarity3 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi199312. 2 interactors.
IntActiQ62431. 1 interactor.
MINTiMINT-4088325.
STRINGi10090.ENSMUSP00000019708.

Structurei

3D structure databases

ProteinModelPortaliQ62431.
SMRiQ62431.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini243 – 335ARIDPROSITE-ProRule annotationAdd BLAST93
Domaini449 – 546REKLESPROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni128 – 165AcidicAdd BLAST38
Regioni450 – 493Important for nuclear localizationAdd BLAST44
Regioni495 – 518HomodimerizationBy similarityAdd BLAST24
Regioni542 – 562Important for cytoplasmic localizationAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi27 – 34Poly-Pro8
Compositional biasi68 – 71Poly-Ala4
Compositional biasi128 – 131Poly-Asp4
Compositional biasi146 – 154Poly-Glu9
Compositional biasi158 – 165Poly-Glu8
Compositional biasi437 – 446Poly-Ala10
Compositional biasi543 – 546Poly-Pro4

Sequence similaritiesi

Contains 1 ARID domain.PROSITE-ProRule annotation
Contains 1 REKLES domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2744. Eukaryota.
ENOG410Y2AP. LUCA.
GeneTreeiENSGT00550000074575.
HOGENOMiHOG000236281.
HOVERGENiHBG050574.
InParanoidiQ62431.
OMAiLFAYSPG.
OrthoDBiEOG091G16XQ.
PhylomeDBiQ62431.
TreeFamiTF320364.

Family and domain databases

Gene3Di1.10.150.60. 1 hit.
InterProiIPR001606. ARID_dom.
IPR023334. REKLES_domain.
[Graphical view]
PfamiPF01388. ARID. 1 hit.
[Graphical view]
SMARTiSM00501. BRIGHT. 1 hit.
[Graphical view]
SUPFAMiSSF46774. SSF46774. 1 hit.
PROSITEiPS51011. ARID. 1 hit.
PS51486. REKLES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62431-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQAVMETL IQRQQRARQE LEARQAPPPP PPEPTGVRAR TTMTDEDREP
60 70 80 90 100
ENARMHRTQM AALAAMRAAA AGLGHPSSPG GSEDGPPISG DEDTAREGTL
110 120 130 140 150
SSPALHGSVL EGAGHAEGDR HLMDVGSDDD DTKSKWEEQE LEELGEEEEE
160 170 180 190 200
EEEEDDFEEE EEEEEGLGPP ESASLGTAGL FTRKAPPAQA FRGDGGPRML
210 220 230 240 250
SGPERLGPGP AHPSHMASQM PPPDHGDWTF EEQFKQLYEL DADPKRKEFL
260 270 280 290 300
DDLFSFMQKR GTPVNRIPIM AKQVLDLFML YVLVTEKGGL VEVINKKLWR
310 320 330 340 350
EITKGLNLPT SITSAAFTLR TQYMKYLYPY ECERRGLSSP NELQAAIDSN
360 370 380 390 400
RREGRRQSFG GSLFAYSPSG AHSMLPSPKL PVTPLGLAAS TNGSSITPAP
410 420 430 440 450
KIKKEEDSAI PITVPGRLPV SLAGHPVVAA QAAAVQAAAA QAAVAAQAAA
460 470 480 490 500
LEQLREKLES TEPPEKKMAL VADEQQRLMQ RAVQQSFLAM TAQLPMNIRI
510 520 530 540 550
NSQASESRQD SAVSLTSANG SNSISMSVEM NGIVYTGVLF AQPPPPTAPS
560 570 580 590 600
APGKGGVSSI GTNTTTGSRT GASGSTVSGG QVGLPGVSTP TMSSTSNNSL

P
Length:601
Mass (Da):64,173
Last modified:November 1, 1996 - v1
Checksum:i66994C01E1FB68CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti405 – 406Missing in AAH50925 (PubMed:15489334).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60335 mRNA. Translation: AAB03416.1.
AK141283 mRNA. Translation: BAE24635.1.
AK154956 mRNA. Translation: BAE32951.1.
BC050925 mRNA. Translation: AAH50925.1.
CCDSiCCDS23999.1.
RefSeqiNP_001275554.1. NM_001288625.1.
NP_001275555.1. NM_001288626.1.
NP_031906.1. NM_007880.4.
XP_006513264.1. XM_006513201.3.
XP_006513265.1. XM_006513202.3.
UniGeneiMm.301282.

Genome annotation databases

EnsembliENSMUST00000019708; ENSMUSP00000019708; ENSMUSG00000019564.
ENSMUST00000105376; ENSMUSP00000101015; ENSMUSG00000019564.
GeneIDi13496.
KEGGimmu:13496.
UCSCiuc007gap.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60335 mRNA. Translation: AAB03416.1.
AK141283 mRNA. Translation: BAE24635.1.
AK154956 mRNA. Translation: BAE32951.1.
BC050925 mRNA. Translation: AAH50925.1.
CCDSiCCDS23999.1.
RefSeqiNP_001275554.1. NM_001288625.1.
NP_001275555.1. NM_001288626.1.
NP_031906.1. NM_007880.4.
XP_006513264.1. XM_006513201.3.
XP_006513265.1. XM_006513202.3.
UniGeneiMm.301282.

3D structure databases

ProteinModelPortaliQ62431.
SMRiQ62431.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199312. 2 interactors.
IntActiQ62431. 1 interactor.
MINTiMINT-4088325.
STRINGi10090.ENSMUSP00000019708.

PTM databases

iPTMnetiQ62431.
PhosphoSitePlusiQ62431.

Proteomic databases

EPDiQ62431.
MaxQBiQ62431.
PaxDbiQ62431.
PeptideAtlasiQ62431.
PRIDEiQ62431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019708; ENSMUSP00000019708; ENSMUSG00000019564.
ENSMUST00000105376; ENSMUSP00000101015; ENSMUSG00000019564.
GeneIDi13496.
KEGGimmu:13496.
UCSCiuc007gap.2. mouse.

Organism-specific databases

CTDi1820.
MGIiMGI:1328360. Arid3a.

Phylogenomic databases

eggNOGiKOG2744. Eukaryota.
ENOG410Y2AP. LUCA.
GeneTreeiENSGT00550000074575.
HOGENOMiHOG000236281.
HOVERGENiHBG050574.
InParanoidiQ62431.
OMAiLFAYSPG.
OrthoDBiEOG091G16XQ.
PhylomeDBiQ62431.
TreeFamiTF320364.

Miscellaneous databases

ChiTaRSiArid3a. mouse.
PROiQ62431.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019564.
CleanExiMM_ARID3A.
ExpressionAtlasiQ62431. baseline and differential.
GenevisibleiQ62431. MM.

Family and domain databases

Gene3Di1.10.150.60. 1 hit.
InterProiIPR001606. ARID_dom.
IPR023334. REKLES_domain.
[Graphical view]
PfamiPF01388. ARID. 1 hit.
[Graphical view]
SMARTiSM00501. BRIGHT. 1 hit.
[Graphical view]
SUPFAMiSSF46774. SSF46774. 1 hit.
PROSITEiPS51011. ARID. 1 hit.
PS51486. REKLES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARI3A_MOUSE
AccessioniPrimary (citable) accession number: Q62431
Secondary accession number(s): Q3U338, Q80YP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.