Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeobox protein Hox-A13

Gene

Hoxa13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific, AT-rich binding transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi320 – 37960HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: MGI
  • sequence-specific DNA binding Source: InterPro
  • sequence-specific DNA binding transcription factor activity Source: MGI
  • transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  • anatomical structure morphogenesis Source: MGI
  • artery morphogenesis Source: MGI
  • branching involved in prostate gland morphogenesis Source: MGI
  • embryonic forelimb morphogenesis Source: MGI
  • endothelial cell fate specification Source: MGI
  • endothelial cell morphogenesis Source: MGI
  • inner ear development Source: MGI
  • male genitalia development Source: MGI
  • organ morphogenesis Source: MGI
  • positive regulation of mesenchymal cell apoptotic process Source: MGI
  • positive regulation of mitotic nuclear division Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of BMP signaling pathway Source: MGI
  • tissue homeostasis Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • vasculogenesis Source: MGI
  • ventricular septum development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Hox-A13
Alternative name(s):
Homeobox protein Hox-1.10
Gene namesi
Name:Hoxa13
Synonyms:Hox-1.10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96173. Hoxa13.

Subcellular locationi

GO - Cellular componenti

  • intermediate filament cytoskeleton Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Hoxa13 are the cause of hypodactyly (Hd), a condition characterized by profound deficiency of digital arch structures.

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi366 – 3661I → A: Decreased DNA binding and transcriptional activity. 1 Publication
Mutagenesisi370 – 3701N → A: Decreased DNA binding and transcriptional activity. 1 Publication
Mutagenesisi373 – 3731V → A: Decreased DNA binding and transcriptional activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Homeobox protein Hox-A13PRO_0000200102Add
BLAST

Proteomic databases

PRIDEiQ62424.

PTM databases

PhosphoSiteiQ62424.

Expressioni

Gene expression databases

CleanExiMM_HOXA13.
GenevestigatoriQ62424.

Interactioni

Subunit structurei

Binds DNA as a homodimer. Interacts with MEIS1, MEIS2 and MEIS3.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Meis1Q60954-23EBI-925160,EBI-445723

Protein-protein interaction databases

BioGridi200366. 4 interactions.
IntActiQ62424. 3 interactions.

Structurei

Secondary structure

1
386
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi329 – 34113Combined sources
Helixi347 – 35711Combined sources
Helixi362 – 38120Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LD5NMR-A320-386[»]
ProteinModelPortaliQ62424.
SMRiQ62424. Positions 320-386.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62424.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi38 – 5114Poly-AlaAdd
BLAST
Compositional biasi52 – 576Poly-Gly
Compositional biasi62 – 665Poly-Ala
Compositional biasi73 – 8412Poly-AlaAdd
BLAST
Compositional biasi101 – 1044Poly-Ala
Compositional biasi116 – 13318Poly-AlaAdd
BLAST
Compositional biasi198 – 2058Poly-Ala

Sequence similaritiesi

Belongs to the Abd-B homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG276440.
HOGENOMiHOG000231176.
HOVERGENiHBG106958.
InParanoidiQ62424.
KOiK09298.
PhylomeDBiQ62424.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR022067. HoxA13_N.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF12284. HoxA13_N. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTASVLLHPR WIEPTVMFLY DNGGGLVADE LNKNMEGAAA AAAAAAAAAA
60 70 80 90 100
AGAGGGGFPH PAAAAAGGNF SVAAAAAAAA AAAANQCRNL MAHPAPLAPG
110 120 130 140 150
AAAAYSSAPG EAPPSAAAAA AAAAAAAAAA AAASSSGGPG PAGPAGAEAA
160 170 180 190 200
KQCSPCSAAA QSSSGPAALP YGYFGSGYYP CARMGPHPNA IKSCAQPASA
210 220 230 240 250
AAAFADKYMD TAGPAAEEFS SRAKEFAFYH QGYAAGPYHH HQPVPGYLDM
260 270 280 290 300
PVVPGLGGPG ESRHEPLGLP MESYQPWALP NGWNGQMYCP KEQTQPPHLW
310 320 330 340 350
KSTLPDVVSH PSDASSYRRG RKKRVPYTKV QLKELEREYA TNKFITKDKR
360 370 380
RRISATTNLS ERQVTIWFQN RRVKEKKVIN KLKTTS
Length:386
Mass (Da):39,567
Last modified:November 1, 1996 - v1
Checksum:i2B01DCC9B1951324
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59322 Genomic DNA. Translation: AAB03322.1.
RefSeqiNP_032290.1. NM_008264.1.
UniGeneiMm.377086.

Genome annotation databases

GeneIDi15398.
KEGGimmu:15398.
UCSCiuc029vvl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59322 Genomic DNA. Translation: AAB03322.1.
RefSeqiNP_032290.1. NM_008264.1.
UniGeneiMm.377086.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LD5NMR-A320-386[»]
ProteinModelPortaliQ62424.
SMRiQ62424. Positions 320-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200366. 4 interactions.
IntActiQ62424. 3 interactions.

PTM databases

PhosphoSiteiQ62424.

Proteomic databases

PRIDEiQ62424.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi15398.
KEGGimmu:15398.
UCSCiuc029vvl.1. mouse.

Organism-specific databases

CTDi3209.
MGIiMGI:96173. Hoxa13.

Phylogenomic databases

eggNOGiNOG276440.
HOGENOMiHOG000231176.
HOVERGENiHBG106958.
InParanoidiQ62424.
KOiK09298.
PhylomeDBiQ62424.

Miscellaneous databases

EvolutionaryTraceiQ62424.
NextBioi288086.
PROiQ62424.
SOURCEiSearch...

Gene expression databases

CleanExiMM_HOXA13.
GenevestigatoriQ62424.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR022067. HoxA13_N.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF12284. HoxA13_N. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The molecular basis of hypodactyly (Hd): a deletion in Hoxa 13 leads to arrest of digital arch formation."
    Mortlock D.P., Post L.C., Innis J.W.
    Nat. Genet. 13:284-289(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Range of HOX/TALE superclass associations and protein domain requirements for HOXA13:MEIS interaction."
    Williams T.M., Williams M.E., Innis J.W.
    Dev. Biol. 277:457-471(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MEIS1; MEIS2 AND MEIS3.
  3. "Structural basis for sequence specific DNA binding and protein dimerization of HOXA13."
    Zhang Y., Larsen C.A., Stadler H.S., Ames J.B.
    PLoS ONE 6:E23069-E23069(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 320-386 IN COMPLEX WITH DNA, SUBUNIT, MUTAGENESIS OF ILE-366; ASN-370 AND VAL-373.

Entry informationi

Entry nameiHXA13_MOUSE
AccessioniPrimary (citable) accession number: Q62424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 1, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Vertebrate homeotic Hox proteins
    Nomenclature of vertebrate homeotic Hox proteins and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.