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Q62424 (HXA13_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein Hox-A13
Alternative name(s):
Homeobox protein Hox-1.10
Gene names
Name:Hoxa13
Synonyms:Hox-1.10
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length386 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sequence-specific, AT-rich binding transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis By similarity.

Subunit structure

Binds DNA as a homodimer. Interacts with MEIS1, MEIS2 and MEIS3. Ref.2 Ref.3

Subcellular location

Nucleus.

Involvement in disease

Defects in Hoxa13 are the cause of hypodactyly (Hd), a condition characterized by profound deficiency of digital arch structures.

Sequence similarities

Belongs to the Abd-B homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanatomical structure morphogenesis

Inferred from mutant phenotype PubMed 12783783. Source: MGI

artery morphogenesis

Inferred from mutant phenotype PubMed 18483557. Source: MGI

branching involved in prostate gland morphogenesis

Inferred from mutant phenotype PubMed 10210434. Source: MGI

embryonic forelimb morphogenesis

Inferred from mutant phenotype PubMed 15733672. Source: MGI

endothelial cell fate specification

Inferred from mutant phenotype PubMed 18483557. Source: MGI

endothelial cell morphogenesis

Inferred from mutant phenotype PubMed 18483557. Source: MGI

inner ear development

Inferred from mutant phenotype PubMed 18483557. Source: MGI

male genitalia development

Inferred from mutant phenotype PubMed 12783783. Source: MGI

organ morphogenesis

Inferred from mutant phenotype PubMed 10210434. Source: MGI

positive regulation of mesenchymal cell apoptotic process

Inferred from mutant phenotype PubMed 12783783. Source: MGI

positive regulation of mitosis

Inferred from mutant phenotype PubMed 12783783. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 16314414. Source: MGI

regulation of BMP signaling pathway

Inferred from mutant phenotype PubMed 12783783. Source: MGI

tissue homeostasis

Inferred from mutant phenotype PubMed 18483557. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

vasculogenesis

Inferred from mutant phenotype PubMed 18483557. Source: MGI

ventricular septum development

Inferred from mutant phenotype PubMed 18483557. Source: MGI

   Cellular_componentnucleus

Inferred from direct assay Ref.2PubMed 15733672. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 19168674. Source: MGI

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 16314414. Source: MGI

transcription regulatory region DNA binding

Inferred from direct assay PubMed 18483557. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Meis1Q60954-23EBI-925160,EBI-445723

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 386386Homeobox protein Hox-A13
PRO_0000200102

Regions

DNA binding320 – 37960Homeobox
Compositional bias38 – 5114Poly-Ala
Compositional bias52 – 576Poly-Gly
Compositional bias62 – 665Poly-Ala
Compositional bias73 – 8412Poly-Ala
Compositional bias101 – 1044Poly-Ala
Compositional bias116 – 13318Poly-Ala
Compositional bias198 – 2058Poly-Ala

Experimental info

Mutagenesis3661I → A: Decreased DNA binding and transcriptional activity. Ref.3
Mutagenesis3701N → A: Decreased DNA binding and transcriptional activity. Ref.3
Mutagenesis3731V → A: Decreased DNA binding and transcriptional activity. Ref.3

Secondary structure

....... 386
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q62424 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 2B01DCC9B1951324

FASTA38639,567
        10         20         30         40         50         60 
MTASVLLHPR WIEPTVMFLY DNGGGLVADE LNKNMEGAAA AAAAAAAAAA AGAGGGGFPH 

        70         80         90        100        110        120 
PAAAAAGGNF SVAAAAAAAA AAAANQCRNL MAHPAPLAPG AAAAYSSAPG EAPPSAAAAA 

       130        140        150        160        170        180 
AAAAAAAAAA AAASSSGGPG PAGPAGAEAA KQCSPCSAAA QSSSGPAALP YGYFGSGYYP 

       190        200        210        220        230        240 
CARMGPHPNA IKSCAQPASA AAAFADKYMD TAGPAAEEFS SRAKEFAFYH QGYAAGPYHH 

       250        260        270        280        290        300 
HQPVPGYLDM PVVPGLGGPG ESRHEPLGLP MESYQPWALP NGWNGQMYCP KEQTQPPHLW 

       310        320        330        340        350        360 
KSTLPDVVSH PSDASSYRRG RKKRVPYTKV QLKELEREYA TNKFITKDKR RRISATTNLS 

       370        380 
ERQVTIWFQN RRVKEKKVIN KLKTTS 

« Hide

References

[1]"The molecular basis of hypodactyly (Hd): a deletion in Hoxa 13 leads to arrest of digital arch formation."
Mortlock D.P., Post L.C., Innis J.W.
Nat. Genet. 13:284-289(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Range of HOX/TALE superclass associations and protein domain requirements for HOXA13:MEIS interaction."
Williams T.M., Williams M.E., Innis J.W.
Dev. Biol. 277:457-471(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MEIS1; MEIS2 AND MEIS3.
[3]"Structural basis for sequence specific DNA binding and protein dimerization of HOXA13."
Zhang Y., Larsen C.A., Stadler H.S., Ames J.B.
PLoS ONE 6:E23069-E23069(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 320-386 IN COMPLEX WITH DNA, SUBUNIT, MUTAGENESIS OF ILE-366; ASN-370 AND VAL-373.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U59322 Genomic DNA. Translation: AAB03322.1.
RefSeqNP_032290.1. NM_008264.1.
UniGeneMm.377086.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2LD5NMR-A320-386[»]
ProteinModelPortalQ62424.
SMRQ62424. Positions 320-386.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200366. 4 interactions.
IntActQ62424. 3 interactions.

PTM databases

PhosphoSiteQ62424.

Proteomic databases

PRIDEQ62424.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID15398.
KEGGmmu:15398.
UCSCuc029vvl.1. mouse.

Organism-specific databases

CTD3209.
MGIMGI:96173. Hoxa13.

Phylogenomic databases

eggNOGNOG276440.
HOGENOMHOG000231176.
HOVERGENHBG106958.
InParanoidQ62424.
KOK09298.
PhylomeDBQ62424.

Gene expression databases

CleanExMM_HOXA13.
GenevestigatorQ62424.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR022067. HoxA13_N.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF12284. HoxA13_N. 2 hits.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ62424.
NextBio288086.
PROQ62424.
SOURCESearch...

Entry information

Entry nameHXA13_MOUSE
AccessionPrimary (citable) accession number: Q62424
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Vertebrate homeotic Hox proteins

Nomenclature of vertebrate homeotic Hox proteins and list of entries