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Protein

Endophilin-A3

Gene

Sh3gl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • synaptic vesicle uncoating Source: SynGO

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endophilin-A3
Alternative name(s):
Endophilin-3
SH3 domain protein 2C
SH3 domain-containing GRB2-like protein 3
SH3p13
Gene namesi
Name:Sh3gl3
Synonyms:Sh3d2c, Sh3d2c2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:700011. Sh3gl3.

Subcellular locationi

  • Cytoplasm By similarity
  • Early endosome membrane By similarity; Peripheral membrane protein By similarity

  • Note: Associated with postsynaptic endosomes in hippocampal neurons. Associated with presynaptic endosomes in olfactory neurons.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • early endosome membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • synapse Source: GOC

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001467511 – 347Endophilin-A3Add BLAST347

Proteomic databases

MaxQBiQ62421.
PaxDbiQ62421.
PeptideAtlasiQ62421.
PRIDEiQ62421.

PTM databases

iPTMnetiQ62421.
PhosphoSitePlusiQ62421.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030638.
CleanExiMM_SH3GL3.

Interactioni

Subunit structurei

Interacts with ARC, DNM1, SGIP1, SYNJ1 and DYDC1. Interacts with FASLG (By similarity). Interacts with ATX2. Interacts with BIN2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203210. 8 interactors.
IntActiQ62421. 2 interactors.
MINTiMINT-238811.
STRINGi10090.ENSMUSP00000032874.

Structurei

3D structure databases

ProteinModelPortaliQ62421.
SMRiQ62421.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 249BARPROSITE-ProRule annotationAdd BLAST232
Domaini285 – 344SH3PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 21Membrane-binding amphipathic helixBy similarityAdd BLAST21
Regioni60 – 87Required for dimerization upon membrane associationBy similarityAdd BLAST28
Regioni218 – 254Interaction with ARCBy similarityAdd BLAST37

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili180 – 201Sequence analysisAdd BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi273 – 276Poly-Ser4

Domaini

An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.By similarity

Sequence similaritiesi

Belongs to the endophilin family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG1118. Eukaryota.
ENOG410XNYB. LUCA.
HOGENOMiHOG000231641.
HOVERGENiHBG052866.
InParanoidiQ62421.
KOiK11247.
PhylomeDBiQ62421.
TreeFamiTF313281.

Family and domain databases

CDDicd07615. BAR_Endophilin_A3. 1 hit.
InterProiView protein in InterPro
IPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR028501. Endophilin-A.
IPR032469. Endophilin-A3_BAR.
IPR011511. SH3_2.
IPR001452. SH3_domain.
PANTHERiPTHR10663:SF260. PTHR10663:SF260. 1 hit.
PfamiView protein in Pfam
PF03114. BAR. 1 hit.
PF07653. SH3_2. 1 hit.
PRINTSiPR00452. SH3DOMAIN.
SMARTiView protein in SMART
SM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
SUPFAMiSSF103657. SSF103657. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiView protein in PROSITE
PS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62421-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVAGLKKQF HKASQLFSEK ISGAEGTKLD EEFLNMEKKI DITSKAVAEI
60 70 80 90 100
LSKATEYLQP NPAYRAKLGM LNTVSKLRGQ VKATGYPQTE GLLGDCMLKY
110 120 130 140 150
GKELGEDSAF GNSLVDVGEA LKLMAEVKDS LDINVKQTFI DPLQLLQDKD
160 170 180 190 200
LKEIGHHLRK LEGRRLDYDY KKRRVGKIPE EEIRQAVEKF EESKELAERS
210 220 230 240 250
MFNFLENDVE QVSQLAVFVE AALDYHRQST EILQELQSKL ELRISLASKV
260 270 280 290 300
PKREFMPKPV NMSSTDANGV GPSSSSKTPG TDTPADQPCC RGLYDFEPEN
310 320 330 340
EGELGFKEGD IITLTNQIDE NWYEGMLRGE SGFFPINYVE VIVPLPP
Length:347
Mass (Da):38,934
Last modified:November 1, 1996 - v1
Checksum:iA2174642F853B5EB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti285A → S in AAH27096 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58887 mRNA. Translation: AAC72268.1.
BC027096 mRNA. Translation: AAH27096.1.
RefSeqiNP_059096.3. NM_017400.6.
UniGeneiMm.432002.
Mm.490720.
Mm.736.

Genome annotation databases

GeneIDi20408.
KEGGimmu:20408.
UCSCiuc029wml.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSH3G3_MOUSE
AccessioniPrimary (citable) accession number: Q62421
Secondary accession number(s): Q8R0B7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: May 10, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families