Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endophilin-A2

Gene

Sh3gl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity).By similarity

GO - Molecular functioni

  1. identical protein binding Source: MGI
  2. lipid binding Source: UniProtKB-KW

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endophilin-A2
Alternative name(s):
Endophilin-2
SH3 domain protein 2B
SH3 domain-containing GRB2-like protein 1
SH3p8
Gene namesi
Name:Sh3gl1
Synonyms:Sh3d2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:700010. Sh3gl1.

Subcellular locationi

Cytoplasm By similarity. Early endosome membrane By similarity; Peripheral membrane protein By similarity. Cell projectionpodosome By similarity
Note: Associated with postsynaptic endosomes in hippocampal neurons.By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cell projection Source: UniProtKB-KW
  3. cytoplasm Source: MGI
  4. early endosome membrane Source: UniProtKB-SubCell
  5. podosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Endophilin-A2PRO_0000146745Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei288 – 2881Phosphoserine1 Publication
Modified residuei315 – 3151Phosphotyrosine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ62419.
PaxDbiQ62419.
PRIDEiQ62419.

PTM databases

PhosphoSiteiQ62419.

Expressioni

Gene expression databases

BgeeiQ62419.
CleanExiMM_SH3GL1.
ExpressionAtlasiQ62419. baseline and differential.
GenevestigatoriQ62419.

Interactioni

Subunit structurei

Interacts with ARC, SYNJ1 and DNM1. Interacts with PDCD6IP. Interacts with BIN2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FauP355453EBI-642935,EBI-309546

Protein-protein interaction databases

BioGridi203207. 4 interactions.
IntActiQ62419. 8 interactions.
MINTiMINT-238674.
STRINGi10090.ENSMUSP00000003268.

Structurei

3D structure databases

ProteinModelPortaliQ62419.
SMRiQ62419. Positions 30-252, 305-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 249232BARPROSITE-ProRule annotationAdd
BLAST
Domaini306 – 36560SH3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2121Membrane-binding amphipathic helixBy similarityAdd
BLAST
Regioni60 – 8728Required for dimerization upon membrane associationBy similarityAdd
BLAST
Regioni218 – 25437Interaction with ARCBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili180 – 25071Sequence AnalysisAdd
BLAST

Domaini

An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.By similarity

Sequence similaritiesi

Belongs to the endophilin family.Curated
Contains 1 BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG307129.
GeneTreeiENSGT00550000074464.
HOGENOMiHOG000231641.
HOVERGENiHBG052866.
InParanoidiQ62419.
KOiK11247.
OMAiLLGECMG.
OrthoDBiEOG7G1V6H.
PhylomeDBiQ62419.
TreeFamiTF313281.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR028501. Endophilin-A.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10661:SF125. PTHR10661:SF125. 1 hit.
PfamiPF03114. BAR. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62419-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVAGLKKQF YKASQLVSEK VGGAEGTKLD DDFKDMEKKV DVTSKAVAEV
60 70 80 90 100
LVRTIEYLQP NPASRAKLTM LNTVSKIRGQ VKNPGYPQSE GLLGECMVRH
110 120 130 140 150
GKELGGESNF GDALLDAGES MKRLAEVKDS LDIEVKQNFI DPLQNLCDKD
160 170 180 190 200
LKEIQHHLKK LEGRRLDFDY KKKRQGKIPD EELRQALEKF EESKEVAETS
210 220 230 240 250
MHNLLETDIE QVSQLSALVD AQLDYHRQAV QILEELADKL KRRVREASSR
260 270 280 290 300
PKREFKPRPR EPFELGELEQ PNGGFPCAPA PKITASSSFR SSDKPIRMPS
310 320 330 340 350
KSMPPLDQPS CKALYDFEPE NDGELGFREG DLITLTNQID ENWYEGMLHG
360
QSGFFPLSYV QVLVPLPQ
Length:368
Mass (Da):41,518
Last modified:November 1, 1996 - v1
Checksum:i3F753B1669086353
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58885 mRNA. Translation: AAC71775.1.
CCDSiCCDS28891.1.
RefSeqiNP_038692.1. NM_013664.3.
UniGeneiMm.1773.

Genome annotation databases

EnsembliENSMUST00000003268; ENSMUSP00000003268; ENSMUSG00000003200.
GeneIDi20405.
KEGGimmu:20405.
UCSCiuc008daq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58885 mRNA. Translation: AAC71775.1.
CCDSiCCDS28891.1.
RefSeqiNP_038692.1. NM_013664.3.
UniGeneiMm.1773.

3D structure databases

ProteinModelPortaliQ62419.
SMRiQ62419. Positions 30-252, 305-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203207. 4 interactions.
IntActiQ62419. 8 interactions.
MINTiMINT-238674.
STRINGi10090.ENSMUSP00000003268.

PTM databases

PhosphoSiteiQ62419.

Proteomic databases

MaxQBiQ62419.
PaxDbiQ62419.
PRIDEiQ62419.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003268; ENSMUSP00000003268; ENSMUSG00000003200.
GeneIDi20405.
KEGGimmu:20405.
UCSCiuc008daq.2. mouse.

Organism-specific databases

CTDi6455.
MGIiMGI:700010. Sh3gl1.

Phylogenomic databases

eggNOGiNOG307129.
GeneTreeiENSGT00550000074464.
HOGENOMiHOG000231641.
HOVERGENiHBG052866.
InParanoidiQ62419.
KOiK11247.
OMAiLLGECMG.
OrthoDBiEOG7G1V6H.
PhylomeDBiQ62419.
TreeFamiTF313281.

Miscellaneous databases

ChiTaRSiSh3gl1. mouse.
NextBioi298376.
PROiQ62419.
SOURCEiSearch...

Gene expression databases

BgeeiQ62419.
CleanExiMM_SH3GL1.
ExpressionAtlasiQ62419. baseline and differential.
GenevestigatoriQ62419.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR028501. Endophilin-A.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10661:SF125. PTHR10661:SF125. 1 hit.
PfamiPF03114. BAR. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins."
    Sparks A.B., Hoffman N.G., McConnell S.J., Fowlkes D.M., Kay B.K.
    Nat. Biotechnol. 14:741-744(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 228-239, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  3. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-315, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSH3G1_MOUSE
AccessioniPrimary (citable) accession number: Q62419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: February 4, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.