Q62419 (SH3G1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endophilin-A2

Alternative name(s):
Endophilin-2
SH3 domain protein 2B
SH3 domain-containing GRB2-like protein 1
SH3p8

Gene names

Name:	Sh3gl1
Synonyms:	Sh3d2b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in endocytosis. May recruit other proteins to membranes with high curvature By similarity.
Subunit structure	Interacts with ARC, SYNJ1 and DNM1. Interacts with PDCD6IP. Interacts with BIN2 By similarity.
Subcellular location	Cytoplasm By similarity. Early endosome membrane; Peripheral membrane protein By similarity. Cell projection › podosome By similarity. Note: Associated with postsynaptic endosomes in hippocampal neurons By similarity.
Domain	An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes By similarity.
Sequence similarities	Belongs to the endophilin family. Contains 1 BAR domain. Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell junction Cell projection Cytoplasm Endosome Membrane
Domain	Coiled coil SH3 domain
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cell projection Inferred from electronic annotation. Source: UniProtKB-KW early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell podosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Endophilin-A2		PRO_0000146745
Regions
	Domain	18 – 249	232	BAR
	Domain	306 – 365	60	SH3
	Region	1 – 21	21	Membrane-binding amphipathic helix By similarity
	Region	60 – 87	28	Required for dimerization upon membrane association By similarity
	Region	218 – 254	37	Interaction with ARC By similarity
	Coiled coil	180 – 250	71	Potential
Amino acid modifications
	Modified residue	288	1	Phosphoserine Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q62419 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3F753B1669086353	FASTA	368	41,518
	10 20 30 40 50 60 MSVAGLKKQF YKASQLVSEK VGGAEGTKLD DDFKDMEKKV DVTSKAVAEV LVRTIEYLQP 70 80 90 100 110 120 NPASRAKLTM LNTVSKIRGQ VKNPGYPQSE GLLGECMVRH GKELGGESNF GDALLDAGES 130 140 150 160 170 180 MKRLAEVKDS LDIEVKQNFI DPLQNLCDKD LKEIQHHLKK LEGRRLDFDY KKKRQGKIPD 190 200 210 220 230 240 EELRQALEKF EESKEVAETS MHNLLETDIE QVSQLSALVD AQLDYHRQAV QILEELADKL 250 260 270 280 290 300 KRRVREASSR PKREFKPRPR EPFELGELEQ PNGGFPCAPA PKITASSSFR SSDKPIRMPS 310 320 330 340 350 360 KSMPPLDQPS CKALYDFEPE NDGELGFREG DLITLTNQID ENWYEGMLHG QSGFFPLSYV QVLVPLPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins." Sparks A.B., Hoffman N.G., McConnell S.J., Fowlkes D.M., Kay B.K. Nat. Biotechnol. 14:741-744(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 228-239, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U58885 mRNA. Translation: AAC71775.1.
IPI	IPI00312752.
RefSeq	NP_038692.1. NM_013664.3.
UniGene	Mm.1773.
3D structure databases
ProteinModelPortal	Q62419.
SMR	Q62419. Positions 30-252, 305-368.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62419. 5 interactions.
MINT	MINT-238674.
STRING	10090.ENSMUSP00000003268.
PTM databases
PhosphoSite	Q62419.
Proteomic databases
PaxDb	Q62419.
PRIDE	Q62419.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000003268; ENSMUSP00000003268; ENSMUSG00000003200.
GeneID	20405.
KEGG	mmu:20405.
Organism-specific databases
CTD	6455.
MGI	MGI:700010. Sh3gl1.
Phylogenomic databases
eggNOG	NOG307129.
GeneTree	ENSGT00550000074464.
HOGENOM	HOG000231641.
HOVERGEN	HBG052866.
InParanoid	Q62419.
KO	K11247.
OMA	GESNFGD.
OrthoDB	EOG4C2H9V.
Gene expression databases
ArrayExpress	Q62419.
Bgee	Q62419.
CleanEx	MM_SH3GL1.
Genevestigator	Q62419.
GermOnline	ENSMUSG00000003200. Mus musculus.
Family and domain databases
Gene3D	1.20.1270.60. 1 hit.
InterPro	IPR027267. AH/BAR-dom. IPR004148. BAR_dom. IPR001452. SH3_domain. [Graphical view]
Pfam	PF03114. BAR. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00452. SH3DOMAIN.
SMART	SM00721. BAR. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS51021. BAR. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SH3GL1. mouse.
NextBio	298376.
SOURCE	Search...

Entry information

Entry name

SH3G1_MOUSE

Accession

Primary (citable) accession number: Q62419

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program