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Q62419 (SH3G1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endophilin-A2
Alternative name(s):
Endophilin-2
SH3 domain protein 2B
SH3 domain-containing GRB2-like protein 1
SH3p8
Gene names
Name:Sh3gl1
Synonyms:Sh3d2b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length368 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in endocytosis. May recruit other proteins to membranes with high curvature By similarity.

Subunit structure

Interacts with ARC, SYNJ1 and DNM1. Interacts with PDCD6IP. Interacts with BIN2 By similarity.

Subcellular location

Cytoplasm By similarity. Early endosome membrane; Peripheral membrane protein By similarity. Cell projectionpodosome By similarity. Note: Associated with postsynaptic endosomes in hippocampal neurons By similarity.

Domain

An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes By similarity.

Sequence similarities

Belongs to the endophilin family.

Contains 1 BAR domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Biological processEndocytosis
   Cellular componentCell junction
Cell projection
Cytoplasm
Endosome
Membrane
   DomainCoiled coil
SH3 domain
   LigandLipid-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processendocytosis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cell projection

Inferred from electronic annotation. Source: UniProtKB-KW

early endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

podosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlipid binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FauP355453EBI-642935,EBI-309546

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 368368Endophilin-A2
PRO_0000146745

Regions

Domain18 – 249232BAR
Domain306 – 36560SH3
Region1 – 2121Membrane-binding amphipathic helix By similarity
Region60 – 8728Required for dimerization upon membrane association By similarity
Region218 – 25437Interaction with ARC By similarity
Coiled coil180 – 25071 Potential

Amino acid modifications

Modified residue2881Phosphoserine Ref.4
Modified residue3151Phosphotyrosine Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q62419 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3F753B1669086353

FASTA36841,518
        10         20         30         40         50         60 
MSVAGLKKQF YKASQLVSEK VGGAEGTKLD DDFKDMEKKV DVTSKAVAEV LVRTIEYLQP 

        70         80         90        100        110        120 
NPASRAKLTM LNTVSKIRGQ VKNPGYPQSE GLLGECMVRH GKELGGESNF GDALLDAGES 

       130        140        150        160        170        180 
MKRLAEVKDS LDIEVKQNFI DPLQNLCDKD LKEIQHHLKK LEGRRLDFDY KKKRQGKIPD 

       190        200        210        220        230        240 
EELRQALEKF EESKEVAETS MHNLLETDIE QVSQLSALVD AQLDYHRQAV QILEELADKL 

       250        260        270        280        290        300 
KRRVREASSR PKREFKPRPR EPFELGELEQ PNGGFPCAPA PKITASSSFR SSDKPIRMPS 

       310        320        330        340        350        360 
KSMPPLDQPS CKALYDFEPE NDGELGFREG DLITLTNQID ENWYEGMLHG QSGFFPLSYV 


QVLVPLPQ 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins."
Sparks A.B., Hoffman N.G., McConnell S.J., Fowlkes D.M., Kay B.K.
Nat. Biotechnol. 14:741-744(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.
[2]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 228-239, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[3]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-315, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U58885 mRNA. Translation: AAC71775.1.
RefSeqNP_038692.1. NM_013664.3.
UniGeneMm.1773.

3D structure databases

ProteinModelPortalQ62419.
SMRQ62419. Positions 30-252, 305-368.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203207. 4 interactions.
IntActQ62419. 8 interactions.
MINTMINT-238674.
STRING10090.ENSMUSP00000003268.

PTM databases

PhosphoSiteQ62419.

Proteomic databases

PaxDbQ62419.
PRIDEQ62419.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000003268; ENSMUSP00000003268; ENSMUSG00000003200.
GeneID20405.
KEGGmmu:20405.
UCSCuc008daq.2. mouse.

Organism-specific databases

CTD6455.
MGIMGI:700010. Sh3gl1.

Phylogenomic databases

eggNOGNOG307129.
GeneTreeENSGT00550000074464.
HOGENOMHOG000231641.
HOVERGENHBG052866.
InParanoidQ62419.
KOK11247.
OMALLGECMG.
OrthoDBEOG7G1V6H.
PhylomeDBQ62419.
TreeFamTF313281.

Gene expression databases

BgeeQ62419.
CleanExMM_SH3GL1.
GenevestigatorQ62419.

Family and domain databases

Gene3D1.20.1270.60. 1 hit.
InterProIPR027267. AH/BAR-dom.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamPF03114. BAR. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSH3GL1. mouse.
NextBio298376.
PROQ62419.
SOURCESearch...

Entry information

Entry nameSH3G1_MOUSE
AccessionPrimary (citable) accession number: Q62419
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot