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Protein

Drebrin-like protein

Gene

Dbnl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.5 Publications

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: UniProtKB
  • cadherin binding Source: MGI
  • protein domain specific binding Source: MGI

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • neuron projection morphogenesis Source: UniProtKB
  • podosome assembly Source: UniProtKB
  • Rac protein signal transduction Source: MGI
  • receptor-mediated endocytosis Source: InterPro
  • ruffle assembly Source: InterPro
  • synapse assembly Source: UniProtKB

Keywordsi

Molecular functionActin-binding
Biological processAdaptive immunity, Endocytosis, Immunity, Transport

Enzyme and pathway databases

ReactomeiR-MMU-264870 Caspase-mediated cleavage of cytoskeletal proteins
R-MMU-6794361 Neurexins and neuroligins
R-MMU-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Drebrin-like protein
Alternative name(s):
Actin-binding protein 1
SH3 domain-containing protein 7
Gene namesi
Name:DbnlImported
Synonyms:Abp1Imported, Sh3p71 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:700006 Dbnl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi340Y → F: Reduces phosphorylation. Abolishes phosphorylation; when associated with F-350. 2 Publications1
Mutagenesisi350Y → F: Reduces phosphorylation. Impairs podosome rosette formation. Abolishes phosphorylation; when associated with F-340. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000797941 – 436Drebrin-like proteinAdd BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphothreonineCombined sources1
Modified residuei160PhosphoserineBy similarity1
Modified residuei176N6-acetyllysineBy similarity1
Modified residuei277PhosphoserineCombined sources1
Modified residuei280PhosphoserineCombined sources1
Modified residuei283PhosphoserineBy similarity1
Modified residuei291PhosphoserineBy similarity1
Modified residuei296N6-acetyllysineBy similarity1
Modified residuei299PhosphothreonineBy similarity1
Modified residuei340Phosphotyrosine2 Publications1
Modified residuei350Phosphotyrosine2 Publications1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei367 – 368Cleavage; by caspase-3By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ62418
PeptideAtlasiQ62418
PRIDEiQ62418

PTM databases

iPTMnetiQ62418
PhosphoSitePlusiQ62418
SwissPalmiQ62418

Expressioni

Tissue specificityi

Detected in hippocampus neurons and in the Purkinje cell layer in cerebellum (at protein level). Predominantly expressed in brain, thymus and spleen. Also found in testis, heart and lung. Little or no expression detected in ovary or muscle.3 Publications

Developmental stagei

In the embryo, expression is high during early development but drops during later development.1 Publication

Gene expression databases

BgeeiENSMUSG00000020476
CleanExiMM_DBNL
GenevisibleiQ62418 MM

Interactioni

Subunit structurei

Interacts with SHANK3, SYN1 and PRAM1 (By similarity). Interacts with SHANK2. Interacts with FGD1, DNM1 and MAP4K1. Interacts with ANKRD54. Interacts with COBL. Interacts with WASL and WIPF1.By similarity8 Publications

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: UniProtKB
  • cadherin binding Source: MGI
  • protein domain specific binding Source: MGI

Protein-protein interaction databases

BioGridi199059, 4 interactors
DIPiDIP-39835N
IntActiQ62418, 7 interactors
MINTiQ62418
STRINGi10090.ENSMUSP00000020769

Structurei

3D structure databases

ProteinModelPortaliQ62418
SMRiQ62418
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 133ADF-HPROSITE-ProRule annotationAdd BLAST132
Domaini377 – 436SH3PROSITE-ProRule annotationAdd BLAST60

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili179 – 233Sequence analysisAdd BLAST55

Domaini

The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.By similarity

Sequence similaritiesi

Belongs to the ABP1 family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG3655 Eukaryota
ENOG410XRVX LUCA
GeneTreeiENSGT00530000062953
HOVERGENiHBG051316
InParanoidiQ62418
KOiK20520
OMAiGYEGQTN
OrthoDBiEOG091G05KP
PhylomeDBiQ62418
TreeFamiTF318935

Family and domain databases

CDDicd11960 SH3_Abp1_eu, 1 hit
Gene3Di3.40.20.10, 1 hit
InterProiView protein in InterPro
IPR002108 ADF-H
IPR029006 ADF-H/Gelsolin-like_dom_sf
IPR029923 Dbnl
IPR035717 Drebrin-like_SH3
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR10829:SF12 PTHR10829:SF12, 1 hit
PfamiView protein in Pfam
PF00241 Cofilin_ADF, 1 hit
PF14604 SH3_9, 1 hit
SMARTiView protein in SMART
SM00102 ADF, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51263 ADF_H, 1 hit
PS50002 SH3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q62418-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVNLSRNGP ALQEAYVRVV TEKSPTDWAL FTYEGNSNDI RVAGTGEGGL
60 70 80 90 100
EELVEELNSG KVMYAFCRVK DPNSGLPKFV LINWTGEGVN DVRKGACANH
110 120 130 140 150
VSTMANFLKG AHVTINARAE EDVEPECIME KVAKASGANY SFHKESTSFQ
160 170 180 190 200
DVGPQAPVGS VYQKTNAISE IKRVGKDNFW AKAEKEEENR RLEEKRRAEE
210 220 230 240 250
ERQRLEEERR ERELQEAARR EQRYQEQHRS AGAPSPSSRT GEPEQEAVSR
260 270 280 290 300
TRQEWESAGQ QAPHPREIFK QKERAMSTTS VTSSQPGKLR SPFLQKQLTQ
310 320 330 340 350
PETSYGREPT APVSRPAAGV CEEPAPSTLS SAQTEEEPTY EVPPEQDTLY
360 370 380 390 400
EEPPLVQQQG AGSEHIDNYM QSQGFSGQGL CARALYDYQA ADDTEISFDP
410 420 430
ENLITGIEVI DEGWWRGYGP DGHFGMFPAN YVELIE
Length:436
Mass (Da):48,700
Last modified:July 19, 2004 - v2
Checksum:i85AEF9781C698A3F
GO
Isoform 21 Publication (identifier: Q62418-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     236-238: Missing.

Show »
Length:433
Mass (Da):48,428
Checksum:i602D3862C446FA4D
GO
Isoform 3Curated (identifier: Q62418-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     235-238: Missing.

Show »
Length:432
Mass (Da):48,341
Checksum:i4A36D3B6E59C707F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30L → F in BAE40714 (PubMed:16141072).Curated1
Sequence conflicti101V → D in BAE31953 (PubMed:16141072).Curated1
Sequence conflicti135A → V in BAE30108 (PubMed:16141072).Curated1
Sequence conflicti135A → V in BAE31033 (PubMed:16141072).Curated1
Sequence conflicti327S → C in BAE22028 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050789235 – 238Missing in isoform 3. 2 Publications4
Alternative sequenceiVSP_050790236 – 238Missing in isoform 2. 3 Publications3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58884 mRNA Translation: AAC52640.1
AY098595 mRNA Translation: AAM28340.1
AK046073 mRNA Translation: BAC32592.1
AK053796 mRNA Translation: BAC35528.1
AK078082 mRNA Translation: BAC37118.1
AK134136 mRNA Translation: BAE22028.1
AK134487 mRNA Translation: BAE22160.1
AK140752 mRNA Translation: BAE24466.1
AK142818 mRNA Translation: BAE25201.1
AK146920 mRNA Translation: BAE27532.1
AK151096 mRNA Translation: BAE30108.1
AK152204 mRNA Translation: BAE31033.1
AK153389 mRNA Translation: BAE31953.1
AK159984 mRNA Translation: BAE35535.1
AK168898 mRNA Translation: BAE40714.1
AK172471 mRNA Translation: BAE43024.1
AL627069 Genomic DNA No translation available.
BC046430 mRNA Translation: AAH46430.1
CCDSiCCDS24403.1 [Q62418-2]
CCDS48746.1 [Q62418-1]
CCDS48747.1 [Q62418-3]
RefSeqiNP_001139780.1, NM_001146308.1 [Q62418-1]
NP_001139781.1, NM_001146309.1 [Q62418-3]
NP_038838.1, NM_013810.3 [Q62418-2]
UniGeneiMm.256925

Genome annotation databases

EnsembliENSMUST00000020769; ENSMUSP00000020769; ENSMUSG00000020476 [Q62418-1]
ENSMUST00000102928; ENSMUSP00000099992; ENSMUSG00000020476 [Q62418-2]
ENSMUST00000109845; ENSMUSP00000105471; ENSMUSG00000020476 [Q62418-3]
GeneIDi13169
KEGGimmu:13169
UCSCiuc007hxb.2 mouse [Q62418-2]
uc007hxc.2 mouse [Q62418-3]
uc007hxd.2 mouse [Q62418-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDBNL_MOUSE
AccessioniPrimary (citable) accession number: Q62418
Secondary accession number(s): Q3TG34
, Q3U5X3, Q3U8I5, Q3UZ33, Q5NCI5, Q5NCI6, Q5NCI7, Q80WP1, Q8BH56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 19, 2004
Last modified: May 23, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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