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Protein

Drebrin-like protein

Gene

Dbnl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.5 Publications

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: UniProtKB
  • cadherin binding involved in cell-cell adhesion Source: MGI
  • protein domain specific binding Source: MGI

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • neuron projection morphogenesis Source: UniProtKB
  • podosome assembly Source: UniProtKB
  • Rac protein signal transduction Source: MGI
  • receptor-mediated endocytosis Source: InterPro
  • ruffle assembly Source: InterPro
  • synapse assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Endocytosis, Immunity, Transport

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-MMU-264870. Caspase-mediated cleavage of cytoskeletal proteins.
R-MMU-6794361. Interactions of neurexins and neuroligins at synapses.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Drebrin-like protein
Alternative name(s):
Actin-binding protein 1
SH3 domain-containing protein 7
Gene namesi
Name:DbnlImported
Synonyms:Abp1Imported, Sh3p71 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:700006. Dbnl.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: MGI
  • cell cortex Source: MGI
  • clathrin-coated vesicle membrane Source: UniProtKB-SubCell
  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB-SubCell
  • dendrite Source: Ensembl
  • early endosome Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • lamellipodium Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • podosome Source: UniProtKB
  • postsynaptic density Source: Ensembl
  • ruffle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi340Y → F: Reduces phosphorylation. Abolishes phosphorylation; when associated with F-350. 2 Publications1
Mutagenesisi350Y → F: Reduces phosphorylation. Impairs podosome rosette formation. Abolishes phosphorylation; when associated with F-340. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000797941 – 436Drebrin-like proteinAdd BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphothreonineCombined sources1
Modified residuei160PhosphoserineBy similarity1
Modified residuei176N6-acetyllysineBy similarity1
Modified residuei277PhosphoserineCombined sources1
Modified residuei280PhosphoserineCombined sources1
Modified residuei283PhosphoserineBy similarity1
Modified residuei291PhosphoserineBy similarity1
Modified residuei296N6-acetyllysineBy similarity1
Modified residuei299PhosphothreonineBy similarity1
Modified residuei340Phosphotyrosine2 Publications1
Modified residuei350Phosphotyrosine2 Publications1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei367 – 368Cleavage; by caspase-3By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ62418.
PeptideAtlasiQ62418.
PRIDEiQ62418.

PTM databases

iPTMnetiQ62418.
PhosphoSitePlusiQ62418.
SwissPalmiQ62418.

Expressioni

Tissue specificityi

Detected in hippocampus neurons and in the Purkinje cell layer in cerebellum (at protein level). Predominantly expressed in brain, thymus and spleen. Also found in testis, heart and lung. Little or no expression detected in ovary or muscle.3 Publications

Developmental stagei

In the embryo, expression is high during early development but drops during later development.1 Publication

Gene expression databases

BgeeiENSMUSG00000020476.
CleanExiMM_DBNL.
GenevisibleiQ62418. MM.

Interactioni

Subunit structurei

Interacts with SHANK3, SYN1 and PRAM1 (By similarity). Interacts with SHANK2. Interacts with FGD1, DNM1 and MAP4K1. Interacts with ANKRD54. Interacts with COBL. Interacts with WASL and WIPF1.By similarity8 Publications

GO - Molecular functioni

  • actin binding Source: MGI
  • actin filament binding Source: UniProtKB
  • cadherin binding involved in cell-cell adhesion Source: MGI
  • protein domain specific binding Source: MGI

Protein-protein interaction databases

BioGridi199059. 3 interactors.
DIPiDIP-39835N.
IntActiQ62418. 6 interactors.
MINTiMINT-100768.
STRINGi10090.ENSMUSP00000020769.

Structurei

3D structure databases

ProteinModelPortaliQ62418.
SMRiQ62418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 133ADF-HPROSITE-ProRule annotationAdd BLAST132
Domaini377 – 436SH3PROSITE-ProRule annotationAdd BLAST60

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili179 – 233Sequence analysisAdd BLAST55

Domaini

The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.By similarity

Sequence similaritiesi

Belongs to the ABP1 family.Curated
Contains 1 ADF-H domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG3655. Eukaryota.
ENOG410XRVX. LUCA.
GeneTreeiENSGT00530000062953.
HOVERGENiHBG051316.
InParanoidiQ62418.
KOiK20520.
OMAiFQDTGPQ.
OrthoDBiEOG091G05KP.
PhylomeDBiQ62418.
TreeFamiTF318935.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR029923. Dbnl.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10829:SF12. PTHR10829:SF12. 1 hit.
PfamiPF00241. Cofilin_ADF. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00102. ADF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51263. ADF_H. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q62418-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVNLSRNGP ALQEAYVRVV TEKSPTDWAL FTYEGNSNDI RVAGTGEGGL
60 70 80 90 100
EELVEELNSG KVMYAFCRVK DPNSGLPKFV LINWTGEGVN DVRKGACANH
110 120 130 140 150
VSTMANFLKG AHVTINARAE EDVEPECIME KVAKASGANY SFHKESTSFQ
160 170 180 190 200
DVGPQAPVGS VYQKTNAISE IKRVGKDNFW AKAEKEEENR RLEEKRRAEE
210 220 230 240 250
ERQRLEEERR ERELQEAARR EQRYQEQHRS AGAPSPSSRT GEPEQEAVSR
260 270 280 290 300
TRQEWESAGQ QAPHPREIFK QKERAMSTTS VTSSQPGKLR SPFLQKQLTQ
310 320 330 340 350
PETSYGREPT APVSRPAAGV CEEPAPSTLS SAQTEEEPTY EVPPEQDTLY
360 370 380 390 400
EEPPLVQQQG AGSEHIDNYM QSQGFSGQGL CARALYDYQA ADDTEISFDP
410 420 430
ENLITGIEVI DEGWWRGYGP DGHFGMFPAN YVELIE
Length:436
Mass (Da):48,700
Last modified:July 19, 2004 - v2
Checksum:i85AEF9781C698A3F
GO
Isoform 21 Publication (identifier: Q62418-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     236-238: Missing.

Show »
Length:433
Mass (Da):48,428
Checksum:i602D3862C446FA4D
GO
Isoform 3Curated (identifier: Q62418-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     235-238: Missing.

Show »
Length:432
Mass (Da):48,341
Checksum:i4A36D3B6E59C707F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30L → F in BAE40714 (PubMed:16141072).Curated1
Sequence conflicti101V → D in BAE31953 (PubMed:16141072).Curated1
Sequence conflicti135A → V in BAE30108 (PubMed:16141072).Curated1
Sequence conflicti135A → V in BAE31033 (PubMed:16141072).Curated1
Sequence conflicti327S → C in BAE22028 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_050789235 – 238Missing in isoform 3. 2 Publications4
Alternative sequenceiVSP_050790236 – 238Missing in isoform 2. 3 Publications3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58884 mRNA. Translation: AAC52640.1.
AY098595 mRNA. Translation: AAM28340.1.
AK046073 mRNA. Translation: BAC32592.1.
AK053796 mRNA. Translation: BAC35528.1.
AK078082 mRNA. Translation: BAC37118.1.
AK134136 mRNA. Translation: BAE22028.1.
AK134487 mRNA. Translation: BAE22160.1.
AK140752 mRNA. Translation: BAE24466.1.
AK142818 mRNA. Translation: BAE25201.1.
AK146920 mRNA. Translation: BAE27532.1.
AK151096 mRNA. Translation: BAE30108.1.
AK152204 mRNA. Translation: BAE31033.1.
AK153389 mRNA. Translation: BAE31953.1.
AK159984 mRNA. Translation: BAE35535.1.
AK168898 mRNA. Translation: BAE40714.1.
AK172471 mRNA. Translation: BAE43024.1.
AL627069 Genomic DNA. Translation: CAI25434.1.
AL627069 Genomic DNA. Translation: CAI25435.1.
AL627069 Genomic DNA. Translation: CAI25436.1.
BC046430 mRNA. Translation: AAH46430.1.
CCDSiCCDS24403.1. [Q62418-2]
CCDS48746.1. [Q62418-1]
CCDS48747.1. [Q62418-3]
RefSeqiNP_001139780.1. NM_001146308.1. [Q62418-1]
NP_001139781.1. NM_001146309.1. [Q62418-3]
NP_038838.1. NM_013810.3. [Q62418-2]
UniGeneiMm.256925.

Genome annotation databases

EnsembliENSMUST00000020769; ENSMUSP00000020769; ENSMUSG00000020476. [Q62418-1]
ENSMUST00000102928; ENSMUSP00000099992; ENSMUSG00000020476. [Q62418-2]
ENSMUST00000109845; ENSMUSP00000105471; ENSMUSG00000020476. [Q62418-3]
GeneIDi13169.
KEGGimmu:13169.
UCSCiuc007hxb.2. mouse. [Q62418-2]
uc007hxc.2. mouse. [Q62418-3]
uc007hxd.2. mouse. [Q62418-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58884 mRNA. Translation: AAC52640.1.
AY098595 mRNA. Translation: AAM28340.1.
AK046073 mRNA. Translation: BAC32592.1.
AK053796 mRNA. Translation: BAC35528.1.
AK078082 mRNA. Translation: BAC37118.1.
AK134136 mRNA. Translation: BAE22028.1.
AK134487 mRNA. Translation: BAE22160.1.
AK140752 mRNA. Translation: BAE24466.1.
AK142818 mRNA. Translation: BAE25201.1.
AK146920 mRNA. Translation: BAE27532.1.
AK151096 mRNA. Translation: BAE30108.1.
AK152204 mRNA. Translation: BAE31033.1.
AK153389 mRNA. Translation: BAE31953.1.
AK159984 mRNA. Translation: BAE35535.1.
AK168898 mRNA. Translation: BAE40714.1.
AK172471 mRNA. Translation: BAE43024.1.
AL627069 Genomic DNA. Translation: CAI25434.1.
AL627069 Genomic DNA. Translation: CAI25435.1.
AL627069 Genomic DNA. Translation: CAI25436.1.
BC046430 mRNA. Translation: AAH46430.1.
CCDSiCCDS24403.1. [Q62418-2]
CCDS48746.1. [Q62418-1]
CCDS48747.1. [Q62418-3]
RefSeqiNP_001139780.1. NM_001146308.1. [Q62418-1]
NP_001139781.1. NM_001146309.1. [Q62418-3]
NP_038838.1. NM_013810.3. [Q62418-2]
UniGeneiMm.256925.

3D structure databases

ProteinModelPortaliQ62418.
SMRiQ62418.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199059. 3 interactors.
DIPiDIP-39835N.
IntActiQ62418. 6 interactors.
MINTiMINT-100768.
STRINGi10090.ENSMUSP00000020769.

PTM databases

iPTMnetiQ62418.
PhosphoSitePlusiQ62418.
SwissPalmiQ62418.

Proteomic databases

PaxDbiQ62418.
PeptideAtlasiQ62418.
PRIDEiQ62418.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020769; ENSMUSP00000020769; ENSMUSG00000020476. [Q62418-1]
ENSMUST00000102928; ENSMUSP00000099992; ENSMUSG00000020476. [Q62418-2]
ENSMUST00000109845; ENSMUSP00000105471; ENSMUSG00000020476. [Q62418-3]
GeneIDi13169.
KEGGimmu:13169.
UCSCiuc007hxb.2. mouse. [Q62418-2]
uc007hxc.2. mouse. [Q62418-3]
uc007hxd.2. mouse. [Q62418-1]

Organism-specific databases

CTDi28988.
MGIiMGI:700006. Dbnl.

Phylogenomic databases

eggNOGiKOG3655. Eukaryota.
ENOG410XRVX. LUCA.
GeneTreeiENSGT00530000062953.
HOVERGENiHBG051316.
InParanoidiQ62418.
KOiK20520.
OMAiFQDTGPQ.
OrthoDBiEOG091G05KP.
PhylomeDBiQ62418.
TreeFamiTF318935.

Enzyme and pathway databases

ReactomeiR-MMU-264870. Caspase-mediated cleavage of cytoskeletal proteins.
R-MMU-6794361. Interactions of neurexins and neuroligins at synapses.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiDbnl. mouse.
PROiQ62418.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020476.
CleanExiMM_DBNL.
GenevisibleiQ62418. MM.

Family and domain databases

Gene3Di3.40.20.10. 1 hit.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR029923. Dbnl.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10829:SF12. PTHR10829:SF12. 1 hit.
PfamiPF00241. Cofilin_ADF. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00102. ADF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51263. ADF_H. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDBNL_MOUSE
AccessioniPrimary (citable) accession number: Q62418
Secondary accession number(s): Q3TG34
, Q3U5X3, Q3U8I5, Q3UZ33, Q5NCI5, Q5NCI6, Q5NCI7, Q80WP1, Q8BH56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 19, 2004
Last modified: November 30, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.