Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q62414

- NDF2_MOUSE

UniProt

Q62414 - NDF2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Neurogenic differentiation factor 2

Gene

Neurod2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter.8 Publications

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. E-box binding Source: UniProtKB
  3. protein heterodimerization activity Source: UniProtKB
  4. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  5. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  6. transcription corepressor activity Source: UniProtKB

GO - Biological processi

  1. associative learning Source: MGI
  2. behavioral fear response Source: MGI
  3. cellular response to calcium ion Source: UniProtKB
  4. cellular response to electrical stimulus Source: UniProtKB
  5. cerebellar cortex development Source: UniProtKB
  6. negative regulation of synapse maturation Source: UniProtKB
  7. neuron development Source: MGI
  8. positive regulation of calcium-mediated signaling Source: UniProtKB
  9. positive regulation of neuron differentiation Source: UniProtKB
  10. positive regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
  11. positive regulation of synapse maturation Source: UniProtKB
  12. positive regulation of synaptic plasticity Source: UniProtKB
  13. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  14. protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neurogenic differentiation factor 2
Short name:
NeuroD2
Alternative name(s):
NeuroD-related factor
Short name:
NDRF
Gene namesi
Name:Neurod2
Synonyms:Ndrf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:107755. Neurod2.

Subcellular locationi

Nucleus 2 PublicationsPROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice exhibit small brains, ataxia, reduced seizure threshold, growth failure, and postnatal premature death. Show excessive apoptosis in central nervous system. Lacks nuclei of the lateral and basolateral amygdala. Organization of cortical neurons into barrel structures is disrupted; thalamocortical axon terminals fail to segregate in the somatosensory cortex.4 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi131 – 1322ER → AQ: Inhibits neurons differentiation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Neurogenic differentiation factor 2PRO_0000127388Add
BLAST

Post-translational modificationi

Ubiquitinated by the APC/C complex; leading to its degradation in neurons. The CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ62414.
PaxDbiQ62414.
PRIDEiQ62414.

PTM databases

PhosphoSiteiQ62414.

Expressioni

Tissue specificityi

Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum (at protein level). Expressed in layers V and VI of the neocortex at postnatal day 1. Expressed in all layers of the neocortex at postnatal day 4. Strongly expressed in layer IV of the neocortex, particularly in the barrel cortex at postnatal day 7. Expressed in the CA1, CA2 and CA3 and dentate gyrus of the hippocampus and many nuclei such as the habenular thalamic nuclei, paraventricular hypothalamic nuclei, amygdala nuclei, and pyramidal nucleus. Expressed in granule cells, molecular layer neurons, and deep cerebella nuclei of the cerebellum. Expressed in brainstem neurons in the external cuneate nucleus and central gray.3 Publications

Developmental stagei

Expressed in dorsal and ventral parts of the lateral, basolateral and basomedial amygdala at 17 and 18 dpc (at protein level). Initially expressed in embryo at 11 dpc, with persistent expression in the nervous system until birth.2 Publications

Gene expression databases

BgeeiQ62414.
CleanExiMM_NEUROD2.
ExpressionAtlasiQ62414. baseline.
GenevestigatoriQ62414.

Interactioni

Subunit structurei

Interacts with TCF3, TCF4 and TCF12. Interacts with CDC20. Efficient DNA-binding and transcription activation require dimerization with another bHLH protein.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ62414.
SMRiQ62414. Positions 123-180.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini122 – 17453bHLHPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi108 – 1147Nuclear localization signalSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi83 – 9210Poly-Glu
Compositional biasi283 – 2864Poly-Gly

Domaini

The C-terminal region is necessary for depolarization-induced and calcium-dependent transcription activation.

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG268401.
GeneTreeiENSGT00680000099860.
HOGENOMiHOG000049256.
HOVERGENiHBG000250.
InParanoidiQ62414.
KOiK09078.
OMAiCINGNFS.
OrthoDBiEOG722J8S.
PhylomeDBiQ62414.
TreeFamiTF315153.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR022575. Neurogenic_DUF.
IPR016637. TF_bHLH_NeuroD.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
PF12533. Neuro_bHLH. 1 hit.
[Graphical view]
PIRSFiPIRSF015618. bHLH_NeuroD. 1 hit.
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62414-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLTRLFSEPG LLSDVPKFAS WGDGDDDEPR SDKGDAPPQP PPAPGSGAPG
60 70 80 90 100
PARAAKPVSL RGGEEIPEPT LAEVKEEGEL GGEEEEEEEE EEGLDEAEGE
110 120 130 140 150
RPKKRGPKKR KMTKARLERS KLRRQKANAR ERNRMHDLNA ALDNLRKVVP
160 170 180 190 200
CYSKTQKLSK IETLRLAKNY IWALSEILRS GKRPDLVSYV QTLCKGLSQP
210 220 230 240 250
TTNLVAGCLQ LNSRNFLTEQ GADGAGRFHG SGGPFAMHPY PYPCSRLAGA
260 270 280 290 300
QCQAAGGLGG GAAHALRTHG YCAAYETLYA AAGGGGASPD YNSSEYEGPL
310 320 330 340 350
SPPLCLNGNF SLKQDSSPDH EKSYHYSMHY SALPGSRPTG HGLVFGSSAV
360 370 380
RGGVHSENLL SYDMHLHHDR GPMYEELNAF FHN
Length:383
Mass (Da):41,493
Last modified:October 10, 2003 - v3
Checksum:i68342005219829B1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411P → S in AAC26057. (PubMed:8816493)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58471 mRNA. Translation: AAC26057.1.
D83507 mRNA. Translation: BAA11931.1.
AB027126 Genomic DNA. Translation: BAA96490.1.
BC058965 mRNA. Translation: AAH58965.1.
CCDSiCCDS25343.1.
PIRiJC4688.
RefSeqiNP_035025.3. NM_010895.3.
UniGeneiMm.4814.

Genome annotation databases

EnsembliENSMUST00000041685; ENSMUSP00000041373; ENSMUSG00000038255.
GeneIDi18013.
KEGGimmu:18013.
UCSCiuc009vai.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U58471 mRNA. Translation: AAC26057.1 .
D83507 mRNA. Translation: BAA11931.1 .
AB027126 Genomic DNA. Translation: BAA96490.1 .
BC058965 mRNA. Translation: AAH58965.1 .
CCDSi CCDS25343.1.
PIRi JC4688.
RefSeqi NP_035025.3. NM_010895.3.
UniGenei Mm.4814.

3D structure databases

ProteinModelPortali Q62414.
SMRi Q62414. Positions 123-180.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q62414.

Proteomic databases

MaxQBi Q62414.
PaxDbi Q62414.
PRIDEi Q62414.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000041685 ; ENSMUSP00000041373 ; ENSMUSG00000038255 .
GeneIDi 18013.
KEGGi mmu:18013.
UCSCi uc009vai.1. mouse.

Organism-specific databases

CTDi 4761.
MGIi MGI:107755. Neurod2.

Phylogenomic databases

eggNOGi NOG268401.
GeneTreei ENSGT00680000099860.
HOGENOMi HOG000049256.
HOVERGENi HBG000250.
InParanoidi Q62414.
KOi K09078.
OMAi CINGNFS.
OrthoDBi EOG722J8S.
PhylomeDBi Q62414.
TreeFami TF315153.

Miscellaneous databases

ChiTaRSi Neurod2. mouse.
NextBioi 293041.
PROi Q62414.
SOURCEi Search...

Gene expression databases

Bgeei Q62414.
CleanExi MM_NEUROD2.
ExpressionAtlasi Q62414. baseline.
Genevestigatori Q62414.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
IPR022575. Neurogenic_DUF.
IPR016637. TF_bHLH_NeuroD.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
PF12533. Neuro_bHLH. 1 hit.
[Graphical view ]
PIRSFi PIRSF015618. bHLH_NeuroD. 1 hit.
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "NeuroD2 and neuroD3: distinct expression patterns and transcriptional activation potentials within the neuroD gene family."
    McCormick M.B., Tamimi R.M., Snider L., Asakura A., Bergstrom D., Tapscott S.J.
    Mol. Cell. Biol. 16:5792-5800(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Tapscott S.J., McCormick M.B., Tamimi R.T.
    Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 338-339.
  3. "Molecular cloning and characterization of a cDNA encoding a novel basic helix-loop-helix protein structurally related to Neuro-D/BHF1."
    Yasunami M., Suzuki K., Maruyama H., Kawakami H., Nagai Y., Hagiwara M., Ohkubo H.
    Biochem. Biophys. Res. Commun. 220:754-758(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  4. "Structure of the mouse NDRF gene and its regulation during neuronal differentiation of P19 cells."
    Oda H., Iwata I., Yasunami M., Ohkubo H.
    Brain Res. Mol. Brain Res. 77:37-46(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C57BL/6.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  6. "Generation of neurons by transient expression of neural bHLH proteins in mammalian cells."
    Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L.
    Development 127:693-702(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF 131-GLU-ARG-132, SUBCELLULAR LOCATION.
  7. "NeuroD2 is necessary for development and survival of central nervous system neurons."
    Olson J.M., Asakura A., Snider L., Hawkes R., Strand A., Stoeck J., Hallahan A., Pritchard J., Tapscott S.J.
    Dev. Biol. 234:174-187(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  8. Cited for: FUNCTION, DNA-BINDING, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  9. "The dosage of the neuroD2 transcription factor regulates amygdala development and emotional learning."
    Lin C.H., Hansen S., Wang Z., Storm D.R., Tapscott S.J., Olson J.M.
    Proc. Natl. Acad. Sci. U.S.A. 102:14877-14882(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CHROMATIN-BINDING, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
  10. "Regulation of thalamocortical patterning and synaptic maturation by NeuroD2."
    Ince-Dunn G., Hall B.J., Hu S.C., Ripley B., Huganir R.L., Olson J.M., Tapscott S.J., Ghosh A.
    Neuron 49:683-695(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  11. "Transduction of NeuroD2 protein induced neural cell differentiation."
    Noda T., Kawamura R., Funabashi H., Mie M., Kobatake E.
    J. Biotechnol. 126:230-236(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  12. "E protein dosage influences brain development more than family member identity."
    Ravanpay A.C., Olson J.M.
    J. Neurosci. Res. 86:1472-1481(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TCF3; TCF4 AND TCF12, DNA-BINDING, DEVELOPMENTAL STAGE.
  13. "A Cdc20-APC ubiquitin signaling pathway regulates presynaptic differentiation."
    Yang Y., Kim A.H., Yamada T., Wu B., Bilimoria P.M., Ikeuchi Y., de la Iglesia N., Shen J., Bonni A.
    Science 326:575-578(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC20, UBIQUITINATION.
  14. "Transcriptional inhibition of REST by NeuroD2 during neuronal differentiation."
    Ravanpay A.C., Hansen S.J., Olson J.M.
    Mol. Cell. Neurosci. 44:178-189(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNDF2_MOUSE
AccessioniPrimary (citable) accession number: Q62414
Secondary accession number(s): Q61952, Q925V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 10, 2003
Last modified: November 26, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3