Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q62414

- NDF2_MOUSE

UniProt

Q62414 - NDF2_MOUSE

Protein

Neurogenic differentiation factor 2

Gene

Neurod2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (10 Oct 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter.8 Publications

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. E-box binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. protein heterodimerization activity Source: UniProtKB
    5. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
    6. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    7. transcription corepressor activity Source: UniProtKB

    GO - Biological processi

    1. associative learning Source: MGI
    2. behavioral fear response Source: MGI
    3. cellular response to calcium ion Source: UniProtKB
    4. cellular response to electrical stimulus Source: UniProtKB
    5. cerebellar cortex development Source: UniProtKB
    6. negative regulation of synapse maturation Source: UniProtKB
    7. neuron development Source: MGI
    8. positive regulation of calcium-mediated signaling Source: UniProtKB
    9. positive regulation of neuron differentiation Source: UniProtKB
    10. positive regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
    11. positive regulation of synapse maturation Source: UniProtKB
    12. positive regulation of synaptic plasticity Source: UniProtKB
    13. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    14. protein ubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Differentiation, Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neurogenic differentiation factor 2
    Short name:
    NeuroD2
    Alternative name(s):
    NeuroD-related factor
    Short name:
    NDRF
    Gene namesi
    Name:Neurod2
    Synonyms:Ndrf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:107755. Neurod2.

    Subcellular locationi

    Nucleus 2 PublicationsPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice exhibit small brains, ataxia, reduced seizure threshold, growth failure, and postnatal premature death. Show excessive apoptosis in central nervous system. Lacks nuclei of the lateral and basolateral amygdala. Organization of cortical neurons into barrel structures is disrupted; thalamocortical axon terminals fail to segregate in the somatosensory cortex.4 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi131 – 1322ER → AQ: Inhibits neurons differentiation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 383383Neurogenic differentiation factor 2PRO_0000127388Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by the APC/C complex; leading to its degradation in neurons. The CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    MaxQBiQ62414.
    PaxDbiQ62414.
    PRIDEiQ62414.

    PTM databases

    PhosphoSiteiQ62414.

    Expressioni

    Tissue specificityi

    Expressed in the external germinal layer (EGL) and internal granular layer (IGL) of the cerebellum (at protein level). Expressed in layers V and VI of the neocortex at postnatal day 1. Expressed in all layers of the neocortex at postnatal day 4. Strongly expressed in layer IV of the neocortex, particularly in the barrel cortex at postnatal day 7. Expressed in the CA1, CA2 and CA3 and dentate gyrus of the hippocampus and many nuclei such as the habenular thalamic nuclei, paraventricular hypothalamic nuclei, amygdala nuclei, and pyramidal nucleus. Expressed in granule cells, molecular layer neurons, and deep cerebella nuclei of the cerebellum. Expressed in brainstem neurons in the external cuneate nucleus and central gray.3 Publications

    Developmental stagei

    Expressed in dorsal and ventral parts of the lateral, basolateral and basomedial amygdala at 17 and 18 dpc (at protein level). Initially expressed in embryo at 11 dpc, with persistent expression in the nervous system until birth.2 Publications

    Gene expression databases

    ArrayExpressiQ62414.
    BgeeiQ62414.
    CleanExiMM_NEUROD2.
    GenevestigatoriQ62414.

    Interactioni

    Subunit structurei

    Interacts with TCF3, TCF4 and TCF12. Interacts with CDC20. Efficient DNA-binding and transcription activation require dimerization with another bHLH protein.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ62414.
    SMRiQ62414. Positions 123-180.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini122 – 17453bHLHPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi108 – 1147Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi83 – 9210Poly-Glu
    Compositional biasi283 – 2864Poly-Gly

    Domaini

    The C-terminal region is necessary for depolarization-induced and calcium-dependent transcription activation.

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG268401.
    GeneTreeiENSGT00680000099860.
    HOGENOMiHOG000049256.
    HOVERGENiHBG000250.
    InParanoidiQ62414.
    KOiK09078.
    OMAiCINGNFS.
    OrthoDBiEOG722J8S.
    PhylomeDBiQ62414.
    TreeFamiTF315153.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR022575. Neurogenic_DUF.
    IPR016637. TF_bHLH_NeuroD.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    PF12533. Neuro_bHLH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015618. bHLH_NeuroD. 1 hit.
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q62414-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLTRLFSEPG LLSDVPKFAS WGDGDDDEPR SDKGDAPPQP PPAPGSGAPG    50
    PARAAKPVSL RGGEEIPEPT LAEVKEEGEL GGEEEEEEEE EEGLDEAEGE 100
    RPKKRGPKKR KMTKARLERS KLRRQKANAR ERNRMHDLNA ALDNLRKVVP 150
    CYSKTQKLSK IETLRLAKNY IWALSEILRS GKRPDLVSYV QTLCKGLSQP 200
    TTNLVAGCLQ LNSRNFLTEQ GADGAGRFHG SGGPFAMHPY PYPCSRLAGA 250
    QCQAAGGLGG GAAHALRTHG YCAAYETLYA AAGGGGASPD YNSSEYEGPL 300
    SPPLCLNGNF SLKQDSSPDH EKSYHYSMHY SALPGSRPTG HGLVFGSSAV 350
    RGGVHSENLL SYDMHLHHDR GPMYEELNAF FHN 383
    Length:383
    Mass (Da):41,493
    Last modified:October 10, 2003 - v3
    Checksum:i68342005219829B1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti41 – 411P → S in AAC26057. (PubMed:8816493)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U58471 mRNA. Translation: AAC26057.1.
    D83507 mRNA. Translation: BAA11931.1.
    AB027126 Genomic DNA. Translation: BAA96490.1.
    BC058965 mRNA. Translation: AAH58965.1.
    CCDSiCCDS25343.1.
    PIRiJC4688.
    RefSeqiNP_035025.3. NM_010895.3.
    UniGeneiMm.4814.

    Genome annotation databases

    EnsembliENSMUST00000041685; ENSMUSP00000041373; ENSMUSG00000038255.
    GeneIDi18013.
    KEGGimmu:18013.
    UCSCiuc009vai.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U58471 mRNA. Translation: AAC26057.1 .
    D83507 mRNA. Translation: BAA11931.1 .
    AB027126 Genomic DNA. Translation: BAA96490.1 .
    BC058965 mRNA. Translation: AAH58965.1 .
    CCDSi CCDS25343.1.
    PIRi JC4688.
    RefSeqi NP_035025.3. NM_010895.3.
    UniGenei Mm.4814.

    3D structure databases

    ProteinModelPortali Q62414.
    SMRi Q62414. Positions 123-180.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q62414.

    Proteomic databases

    MaxQBi Q62414.
    PaxDbi Q62414.
    PRIDEi Q62414.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000041685 ; ENSMUSP00000041373 ; ENSMUSG00000038255 .
    GeneIDi 18013.
    KEGGi mmu:18013.
    UCSCi uc009vai.1. mouse.

    Organism-specific databases

    CTDi 4761.
    MGIi MGI:107755. Neurod2.

    Phylogenomic databases

    eggNOGi NOG268401.
    GeneTreei ENSGT00680000099860.
    HOGENOMi HOG000049256.
    HOVERGENi HBG000250.
    InParanoidi Q62414.
    KOi K09078.
    OMAi CINGNFS.
    OrthoDBi EOG722J8S.
    PhylomeDBi Q62414.
    TreeFami TF315153.

    Miscellaneous databases

    ChiTaRSi NEUROD2. mouse.
    NextBioi 293041.
    PROi Q62414.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q62414.
    Bgeei Q62414.
    CleanExi MM_NEUROD2.
    Genevestigatori Q62414.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR022575. Neurogenic_DUF.
    IPR016637. TF_bHLH_NeuroD.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    PF12533. Neuro_bHLH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015618. bHLH_NeuroD. 1 hit.
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "NeuroD2 and neuroD3: distinct expression patterns and transcriptional activation potentials within the neuroD gene family."
      McCormick M.B., Tamimi R.M., Snider L., Asakura A., Bergstrom D., Tapscott S.J.
      Mol. Cell. Biol. 16:5792-5800(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Tapscott S.J., McCormick M.B., Tamimi R.T.
      Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 338-339.
    3. "Molecular cloning and characterization of a cDNA encoding a novel basic helix-loop-helix protein structurally related to Neuro-D/BHF1."
      Yasunami M., Suzuki K., Maruyama H., Kawakami H., Nagai Y., Hagiwara M., Ohkubo H.
      Biochem. Biophys. Res. Commun. 220:754-758(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Embryo.
    4. "Structure of the mouse NDRF gene and its regulation during neuronal differentiation of P19 cells."
      Oda H., Iwata I., Yasunami M., Ohkubo H.
      Brain Res. Mol. Brain Res. 77:37-46(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C57BL/6.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    6. "Generation of neurons by transient expression of neural bHLH proteins in mammalian cells."
      Farah M.H., Olson J.M., Sucic H.B., Hume R.I., Tapscott S.J., Turner D.L.
      Development 127:693-702(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF 131-GLU-ARG-132, SUBCELLULAR LOCATION.
    7. "NeuroD2 is necessary for development and survival of central nervous system neurons."
      Olson J.M., Asakura A., Snider L., Hawkes R., Strand A., Stoeck J., Hallahan A., Pritchard J., Tapscott S.J.
      Dev. Biol. 234:174-187(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    8. Cited for: FUNCTION, DNA-BINDING, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    9. "The dosage of the neuroD2 transcription factor regulates amygdala development and emotional learning."
      Lin C.H., Hansen S., Wang Z., Storm D.R., Tapscott S.J., Olson J.M.
      Proc. Natl. Acad. Sci. U.S.A. 102:14877-14882(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CHROMATIN-BINDING, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
    10. "Regulation of thalamocortical patterning and synaptic maturation by NeuroD2."
      Ince-Dunn G., Hall B.J., Hu S.C., Ripley B., Huganir R.L., Olson J.M., Tapscott S.J., Ghosh A.
      Neuron 49:683-695(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    11. "Transduction of NeuroD2 protein induced neural cell differentiation."
      Noda T., Kawamura R., Funabashi H., Mie M., Kobatake E.
      J. Biotechnol. 126:230-236(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    12. "E protein dosage influences brain development more than family member identity."
      Ravanpay A.C., Olson J.M.
      J. Neurosci. Res. 86:1472-1481(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TCF3; TCF4 AND TCF12, DNA-BINDING, DEVELOPMENTAL STAGE.
    13. "A Cdc20-APC ubiquitin signaling pathway regulates presynaptic differentiation."
      Yang Y., Kim A.H., Yamada T., Wu B., Bilimoria P.M., Ikeuchi Y., de la Iglesia N., Shen J., Bonni A.
      Science 326:575-578(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC20, UBIQUITINATION.
    14. "Transcriptional inhibition of REST by NeuroD2 during neuronal differentiation."
      Ravanpay A.C., Hansen S.J., Olson J.M.
      Mol. Cell. Neurosci. 44:178-189(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiNDF2_MOUSE
    AccessioniPrimary (citable) accession number: Q62414
    Secondary accession number(s): Q61952, Q925V5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: October 10, 2003
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3