Q62407 (SPEG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Striated muscle-specific serine/threonine-protein kinase
EC=2.7.11.1

Alternative name(s):
Aortic preferentially expressed protein 1
Short name=APEG-1

Gene names

Name:	Speg
Synonyms:	Apeg1, Kiaa1297

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	3262 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Isoform 3 may have a role in regulating the growth and differentiation of arterial smooth muscle cells.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Isoform 3 is found as a monomer or homodimer By similarity.
Subcellular location	Isoform 3: Nucleus Ref.1.
Tissue specificity	Isoform 1 is preferentially expressed in striated muscle. Non-kinase form such as isoform 3 is predominantly expressed in the aorta. Isoform 3 appears to be expressed only in highly differentiated ASMC in normal vessel walls and down-regulated in dedifferentiated ASMC in vivo. In response to vascular injuries ASMC dedifferentiate and change from a quiescent and contractile phenotype to a proliferative and synthetic phenotype. This proliferation of vascular smooth muscle cells is one of the most prominent features of atherosclerosis. Ref.1 Ref.2
Induction	Isoform 3 is quickly down-regulated in response to vascular injury, when ASMC cells change from a quiescent to a proliferative phenotype. Ref.1
Post-translational modification	May be autophosphorylated. Ref.2
Miscellaneous	Expression is under the tight control of the locus control region (LCRs).
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 2 fibronectin type-III domains. Contains 9 Ig-like (immunoglobulin-like) domains. Contains 2 protein kinase domains.
Sequence caution	The sequence AAG34791.1 differs from that shown. Reason: Frameshift at positions 2957 and 2992.

Ontologies

Keywords
Biological process	Differentiation
Cellular component	Nucleus
Coding sequence diversity	Alternative promoter usage Alternative splicing
Domain	Immunoglobulin domain Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle cell development Inferred from mutant phenotype PubMed 19118250. Source: MGI in utero embryonic development Inferred from mutant phenotype PubMed 19118250. Source: MGI respiratory system development Inferred from mutant phenotype PubMed 19118250. Source: MGI
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q62407-1)

Also known as: SPEG-beta;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q62407-2)

Also known as: BPEG;

The sequence of this isoform differs from the canonical sequence as follows:
     1-106: Missing.
     107-131: VYSCSAQNERGQASCEAVLTVLEVR → MKKLWVKKRFQKTGHSRRAFGRLTH
     966-967: AH → GE
     968-3262: Missing.

Isoform 3 (identifier: Q62407-3)

Also known as: APEG1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-854: Missing.
     966-967: AH → GE

Note: Produced by alternative promoter usage.

Isoform 4 (identifier: Q62407-4)

Also known as: SPEG-alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     1-854: Missing.

Note: No experimental confirmation available. Produced by alternative promoter usage.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 3262	3262	Striated muscle-specific serine/threonine-protein kinase		PRO_0000072667
Regions
	Domain	45 – 126	82	Ig-like 1
	Domain	727 – 815	89	Ig-like 2
	Domain	874 – 963	90	Ig-like 3
	Domain	968 – 1062	95	Ig-like 4
	Domain	1069 – 1157	89	Ig-like 5
	Domain	1193 – 1283	91	Ig-like 6
	Domain	1287 – 1382	96	Fibronectin type-III 1
	Domain	1389 – 1485	97	Ig-like 7
	Domain	1490 – 1578	89	Ig-like 8
	Domain	1606 – 1859	254	Protein kinase 1
	Domain	2586 – 2676	91	Ig-like 9
	Domain	2681 – 2772	92	Fibronectin type-III 2
	Domain	2946 – 3213	268	Protein kinase 2
	Nucleotide binding	1612 – 1620	9	ATP By similarity
	Compositional bias	277 – 348	72	Pro-rich
	Compositional bias	530 – 639	110	Pro-rich
	Compositional bias	1924 – 1929	6	Poly-Ser
	Compositional bias	1930 – 1936	7	Poly-Glu
	Compositional bias	2180 – 2320	141	Pro-rich
	Compositional bias	2775 – 2962	188	Pro-rich
	Compositional bias	3241 – 3244	4	Poly-Arg
Sites
	Active site	1724	1	Proton acceptor By similarity
	Active site	3080	1	Proton acceptor By similarity
	Binding site	1635	1	ATP By similarity
Amino acid modifications
	Modified residue	316	1	Phosphoserine Ref.7
	Modified residue	439	1	Phosphoserine Ref.8
	Modified residue	481	1	Phosphoserine Ref.6
	Modified residue	1177	1	Phosphoserine Ref.6
	Disulfide bond	994 ↔ 1046		By similarity
	Disulfide bond	1413 ↔ 1469		By similarity
	Disulfide bond	2608 ↔ 2660		By similarity
Natural variations
	Alternative sequence	1 – 854	854	Missing in isoform 3 and isoform 4.		VSP_018264
	Alternative sequence	1 – 106	106	Missing in isoform 2.		VSP_018265
	Alternative sequence	107 – 131	25	VYSCS…VLEVR → MKKLWVKKRFQKTGHSRRAF GRLTH in isoform 2.		VSP_018266
	Alternative sequence	966 – 967	2	AH → GE in isoform 2 and isoform 3.		VSP_018267
	Alternative sequence	968 – 3262	2295	Missing in isoform 2.		VSP_018268
Experimental info
	Sequence conflict	3237	1	L → R in BAC65770. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (SPEG-beta) [UniParc]. Last modified May 16, 2006. Version 2. Checksum: 0387BDD6518B7BB7	FASTA	3,262	354,343
	10 20 30 40 50 60 MQKARGTRGE DAGTRAPPSP GVPPKRAKVG AGRGVLVTGD GAGAPVFLRP LKNAAVCAGS 70 80 90 100 110 120 DVRLRVVVSG TPQPSLSWFR DGQLLPPPAP EPSCLWLRSC GAQDAGVYSC SAQNERGQAS 130 140 150 160 170 180 CEAVLTVLEV RDSETAEDDI SDVPGTQRLE LRDDRAFSTP TGGSDTLVGT SLDTPPTSVT 190 200 210 220 230 240 GTSEEQVSWW GSGQTVLEQE AGSGGGTRPL PGSPRQAQTT GAGPRHLGVE PLVRASRANL 250 260 270 280 290 300 VGASWGSEDS LSVASDLYGS AFSLYRGRAL SIHVSIPPSG LHREEPDLQP QPASDALRPR 310 320 330 340 350 360 PALPPPSKSA LLPPPSPRVG KRALPGPSTQ PPATPTSPHR RAQEPSLPED ITTTEEKRGK 370 380 390 400 410 420 KPKSSGPSLA GTVESRPQTP LSEASGRLSA LGRSPRLVRA GSRILDKLQF FEERRRSLER 430 440 450 460 470 480 SDSPPAPLRP WVPLRKARSL EQPKSEGGAA WGTPEASQEE LRSPRGSVAE RRRLFQQKAA 490 500 510 520 530 540 SLDERTRQRS ATSDLELRFA QELGRIRRST SREELVRSHE SLRATLQRAP SPREPGEPPL 550 560 570 580 590 600 FSRPSTPKTS RAVSPAATQP PPPSGAGKSG DEPGRPRSRG PVGRTEPGEG PQQEIKRRDQ 610 620 630 640 650 660 FPLTRSRAIQ ECRSPVPPYT ADPPESRTKA PSGRKREPPA QAVRFLPWAT PGVEDSVLPQ 670 680 690 700 710 720 TLEKNRAGPE AEKRLRRGPE EDGPWGPWDR RGTRSQGKGR RARPTSPELE SSDDSYVSAG 730 740 750 760 770 780 EEPLEAPVFE IPLQNMVVAP GADVLLKCII TANPPPQVSW KKDGSMLHSE GRLLIRAEGE 790 800 810 820 830 840 RHTLLLREAQ AADAGSYTAT ATNELGQATC ASSLAVRPGG STSPFSSPIT SDEEYLSPPE 850 860 870 880 890 900 EFPEPGETWP RTPTMKLSPS QDHDSSDSSS KAPPTFKVSL MDQSVREGQD VIMSIRVQGE 910 920 930 940 950 960 PKPVVSWLRN RQPVRPDQRR FAEEAEGGLC RLRILAAERG DAGFYTCKAV NEYGARQCEA 970 980 990 1000 1010 1020 RLEVRAHPES RSLAVLAPLQ DVDVGAGEMA LFECLVAGPA DVEVDWLCRG RLLQPALLKC 1030 1040 1050 1060 1070 1080 KMHFDGRKCK LLLTSVHEDD SGVYTCKLST AKDELTCSAR LTVRPSLAPL FTRLLEDVEV 1090 1100 1110 1120 1130 1140 LEGRAARLDC KISGTPPPSV TWTHFGHPVN EGDNLRLRQD GGLHSLHIAR VGSEDEGLYE 1150 1160 1170 1180 1190 1200 VSATNTHGQA HCSAQLYVEE PRTAASGPSS KLEKMPSIPE EPEHGDLERL SIPDFLRPLQ 1210 1220 1230 1240 1250 1260 DLEVGLAKEA MLECQVTGLP YPTISWFHNG HRIQSSDDRR MTQYRDIHRL VFPAVGPQHA 1270 1280 1290 1300 1310 1320 GVYKSVIANK LGKAACYAHL YVTDVVPGPP DGAPEVVAVT GRMVTLSWNP PRSLDMAIDP 1330 1340 1350 1360 1370 1380 DSLTYTVQHQ VLGSDQWTAL VTGLREPAWA ATGLKKGIQH IFRVLSSSGK SSSKPSAPSE 1390 1400 1410 1420 1430 1440 PVQLLEHGPP LEEAPAVLDK QDIVYVVEGQ PACVTVTFNH VEAQVVWRSC RGALLEARTG 1450 1460 1470 1480 1490 1500 VYELSQPDDD QYCLRICRVS RRDLGPLTCS ARNRHGTKAC SVTLELAEAP RFESIMEDVE 1510 1520 1530 1540 1550 1560 VGPGETARFA VVVEGKPLPD IMWYKDEVLL AESNHVSFVY EENECSLVLL SAGSQDGGVY 1570 1580 1590 1600 1610 1620 TCTARNLAGE VSCKAELSVL SAQTAMEVEG VGEDEEHRGR RLSDYYDIHQ EIGRGAFSYL 1630 1640 1650 1660 1670 1680 RRVVERSSGL EFAAKFIPSQ AKPKASARRE ARLLARLQHG CVLYFHEAFE RRRGLVIVTE 1690 1700 1710 1720 1730 1740 LCTEELLERM ARKPTVCESE TRTYMRQVLE GICYLHQSHV LHLDVKPENL LVWDGAGGEE 1750 1760 1770 1780 1790 1800 QVRICDFGNA QELTPGEPQY CQYGTPEFVA PEIVNQSPVS GVTDIWPVGV VAFLCLTGIS 1810 1820 1830 1840 1850 1860 PFVGENDRTT LMNIRNYNVA FEETTFLSLS REARGFLIKV LVQDRLRPTA EETLEHPWFK 1870 1880 1890 1900 1910 1920 TEAKGAEVST DHLKLFLSRR RWQRSQISYK CHLVLRPIPE LLRAPPERVW VAMPRRQPPS 1930 1940 1950 1960 1970 1980 GGLSSSSDSE EEELEELPSV PRPLQPEFSG SRVSLTDIPT EDEALGTPEA GAATPMDWQE 1990 2000 2010 2020 2030 2040 QERTPSKDQE APSPEALPSP GQESPDGPSP RRPELRRGSS AESALPRVGS REPGRSLHKA 2050 2060 2070 2080 2090 2100 ASVELPQRRS PSPGATRLTR GGLGEGEYAQ RLQALRQRLL RGGPEDGKVS GLRGPLLESL 2110 2120 2130 2140 2150 2160 GGRARDPRMA RAASSEAAPH HQPPPESRGL QKSSSFSQGE AEPRGRHRRA GAPLEIPVAR 2170 2180 2190 2200 2210 2220 LGARRLQESP SLSALSETQP PSPARPSVPK LSITKSPEPS AVTSRDSPQP PEPQPVPEKV 2230 2240 2250 2260 2270 2280 PEPKPEPVRA AKPAQPPLAL QMPTQPLTPY AQIMQSLQLS SPTLSPQDPA VPPSEPKPHA 2290 2300 2310 2320 2330 2340 AVFARVASPP PGVSEKRVPS ARTPPVLAEK ARVPTVPPRP GSSLSGSIEN LESEAVFEAK 2350 2360 2370 2380 2390 2400 FKRSRESPLS RGLRLLSRSR SEERGPFRGA EDDGIYRPSP AGTPLELVRR PERSRSVQDL 2410 2420 2430 2440 2450 2460 RVAGEPGLVR RLSLSLSQKL RRTPPGQRHP AWESRSGDGE SSEGGSSARA SPVLAVRRRL 2470 2480 2490 2500 2510 2520 SSTLERLSSR LQRSGSSEDS GGASGRSTPL FGRLRRATSE GESLRRLGVP HNQLGSQTGA 2530 2540 2550 2560 2570 2580 TTPSAESLGS EASGTSGSSA PGESRSRHRW GLSRLRKDKG LSQPNLSSSV QEDLGHQYVP 2590 2600 2610 2620 2630 2640 SESDFPPVFH IKLKDQVLLE GEAATLLCLP AACPAPRISW MKDKQSLRSE PSVVIVSCKD 2650 2660 2670 2680 2690 2700 GRQLLSIPRA GKRHAGLYEC SATNVLGSIT SSCTVAVARI PGKLAPPEVP QTYHDTALVV 2710 2720 2730 2740 2750 2760 WKPGDGRAPC TYTLERRVDG ESVWHPVSSG IPDCYYNVTQ LPVGVTVRFR VACSNRAGQG 2770 2780 2790 2800 2810 2820 PFSNPSEKVF IRGTPDSPAQ PAAAPRDAPV TSGPTRAPPP DSPTSLAPTP ALAPPASQAS 2830 2840 2850 2860 2870 2880 TLSPSTSSMS ANQALSSLKA VGPPPATPPR KHRGLLATQQ AEPSPPSIVV TPSEPRSFVP 2890 2900 2910 2920 2930 2940 DTGTLTPTSS PQGVKPAPSS TSLYMVTSFV SAPPAPQAPA PEPPPEPTKV TVRSLSPAKE 2950 2960 2970 2980 2990 3000 VVSSPTPEST TLRQGPPQKP YTFLEEKARG RFGVVRSCRE NATGRTFVAK IVPYAAEGKR 3010 3020 3030 3040 3050 3060 RVLQEYEVLR TLHHERLMSL HEAYITPRYL VLIAESCGNR ELLCGLSDRF RYSEDDVATY 3070 3080 3090 3100 3110 3120 VVQLLQGLDY LHGHHVLHLD IKPDNLLLAA DNALKIVDFG SAQPYNPQAL KPLGHRTGTL 3130 3140 3150 3160 3170 3180 EFMAPEMVKG DPIGSATDIW GAGVLTYIML SGYSPFYEPD PQETEARIVG GRFDAFQLYP 3190 3200 3210 3220 3230 3240 NTSQSATLFL RKVLSVHPWS RPSLQDCLAH PWLQDAYLMK LRRQTLTFTT NRLKEFLGEQ 3250 3260 RRRRAEAATR HKVLLRSYPG SP
	Isoform 2 (BPEG) [UniParc]. Checksum: EA162A24DDC7C597	FASTA	861	93,689
	10 20 30 40 50 60 MKKLWVKKRF QKTGHSRRAF GRLTHDSETA EDDISDVPGT QRLELRDDRA FSTPTGGSDT 70 80 90 100 110 120 LVGTSLDTPP TSVTGTSEEQ VSWWGSGQTV LEQEAGSGGG TRPLPGSPRQ AQTTGAGPRH 130 140 150 160 170 180 LGVEPLVRAS RANLVGASWG SEDSLSVASD LYGSAFSLYR GRALSIHVSI PPSGLHREEP 190 200 210 220 230 240 DLQPQPASDA LRPRPALPPP SKSALLPPPS PRVGKRALPG PSTQPPATPT SPHRRAQEPS 250 260 270 280 290 300 LPEDITTTEE KRGKKPKSSG PSLAGTVESR PQTPLSEASG RLSALGRSPR LVRAGSRILD 310 320 330 340 350 360 KLQFFEERRR SLERSDSPPA PLRPWVPLRK ARSLEQPKSE GGAAWGTPEA SQEELRSPRG 370 380 390 400 410 420 SVAERRRLFQ QKAASLDERT RQRSATSDLE LRFAQELGRI RRSTSREELV RSHESLRATL 430 440 450 460 470 480 QRAPSPREPG EPPLFSRPST PKTSRAVSPA ATQPPPPSGA GKSGDEPGRP RSRGPVGRTE 490 500 510 520 530 540 PGEGPQQEIK RRDQFPLTRS RAIQECRSPV PPYTADPPES RTKAPSGRKR EPPAQAVRFL 550 560 570 580 590 600 PWATPGVEDS VLPQTLEKNR AGPEAEKRLR RGPEEDGPWG PWDRRGTRSQ GKGRRARPTS 610 620 630 640 650 660 PELESSDDSY VSAGEEPLEA PVFEIPLQNM VVAPGADVLL KCIITANPPP QVSWKKDGSM 670 680 690 700 710 720 LHSEGRLLIR AEGERHTLLL REAQAADAGS YTATATNELG QATCASSLAV RPGGSTSPFS 730 740 750 760 770 780 SPITSDEEYL SPPEEFPEPG ETWPRTPTMK LSPSQDHDSS DSSSKAPPTF KVSLMDQSVR 790 800 810 820 830 840 EGQDVIMSIR VQGEPKPVVS WLRNRQPVRP DQRRFAEEAE GGLCRLRILA AERGDAGFYT 850 860 CKAVNEYGAR QCEARLEVRG E
	Isoform 3 (APEG1) [UniParc]. Checksum: 639C18C8D114F389	FASTA	2,408	262,876
	10 20 30 40 50 60 MKLSPSQDHD SSDSSSKAPP TFKVSLMDQS VREGQDVIMS IRVQGEPKPV VSWLRNRQPV 70 80 90 100 110 120 RPDQRRFAEE AEGGLCRLRI LAAERGDAGF YTCKAVNEYG ARQCEARLEV RGEPESRSLA 130 140 150 160 170 180 VLAPLQDVDV GAGEMALFEC LVAGPADVEV DWLCRGRLLQ PALLKCKMHF DGRKCKLLLT 190 200 210 220 230 240 SVHEDDSGVY TCKLSTAKDE LTCSARLTVR PSLAPLFTRL LEDVEVLEGR AARLDCKISG 250 260 270 280 290 300 TPPPSVTWTH FGHPVNEGDN LRLRQDGGLH SLHIARVGSE DEGLYEVSAT NTHGQAHCSA 310 320 330 340 350 360 QLYVEEPRTA ASGPSSKLEK MPSIPEEPEH GDLERLSIPD FLRPLQDLEV GLAKEAMLEC 370 380 390 400 410 420 QVTGLPYPTI SWFHNGHRIQ SSDDRRMTQY RDIHRLVFPA VGPQHAGVYK SVIANKLGKA 430 440 450 460 470 480 ACYAHLYVTD VVPGPPDGAP EVVAVTGRMV TLSWNPPRSL DMAIDPDSLT YTVQHQVLGS 490 500 510 520 530 540 DQWTALVTGL REPAWAATGL KKGIQHIFRV LSSSGKSSSK PSAPSEPVQL LEHGPPLEEA 550 560 570 580 590 600 PAVLDKQDIV YVVEGQPACV TVTFNHVEAQ VVWRSCRGAL LEARTGVYEL SQPDDDQYCL 610 620 630 640 650 660 RICRVSRRDL GPLTCSARNR HGTKACSVTL ELAEAPRFES IMEDVEVGPG ETARFAVVVE 670 680 690 700 710 720 GKPLPDIMWY KDEVLLAESN HVSFVYEENE CSLVLLSAGS QDGGVYTCTA RNLAGEVSCK 730 740 750 760 770 780 AELSVLSAQT AMEVEGVGED EEHRGRRLSD YYDIHQEIGR GAFSYLRRVV ERSSGLEFAA 790 800 810 820 830 840 KFIPSQAKPK ASARREARLL ARLQHGCVLY FHEAFERRRG LVIVTELCTE ELLERMARKP 850 860 870 880 890 900 TVCESETRTY MRQVLEGICY LHQSHVLHLD VKPENLLVWD GAGGEEQVRI CDFGNAQELT 910 920 930 940 950 960 PGEPQYCQYG TPEFVAPEIV NQSPVSGVTD IWPVGVVAFL CLTGISPFVG ENDRTTLMNI 970 980 990 1000 1010 1020 RNYNVAFEET TFLSLSREAR GFLIKVLVQD RLRPTAEETL EHPWFKTEAK GAEVSTDHLK 1030 1040 1050 1060 1070 1080 LFLSRRRWQR SQISYKCHLV LRPIPELLRA PPERVWVAMP RRQPPSGGLS SSSDSEEEEL 1090 1100 1110 1120 1130 1140 EELPSVPRPL QPEFSGSRVS LTDIPTEDEA LGTPEAGAAT PMDWQEQERT PSKDQEAPSP 1150 1160 1170 1180 1190 1200 EALPSPGQES PDGPSPRRPE LRRGSSAESA LPRVGSREPG RSLHKAASVE LPQRRSPSPG 1210 1220 1230 1240 1250 1260 ATRLTRGGLG EGEYAQRLQA LRQRLLRGGP EDGKVSGLRG PLLESLGGRA RDPRMARAAS 1270 1280 1290 1300 1310 1320 SEAAPHHQPP PESRGLQKSS SFSQGEAEPR GRHRRAGAPL EIPVARLGAR RLQESPSLSA 1330 1340 1350 1360 1370 1380 LSETQPPSPA RPSVPKLSIT KSPEPSAVTS RDSPQPPEPQ PVPEKVPEPK PEPVRAAKPA 1390 1400 1410 1420 1430 1440 QPPLALQMPT QPLTPYAQIM QSLQLSSPTL SPQDPAVPPS EPKPHAAVFA RVASPPPGVS 1450 1460 1470 1480 1490 1500 EKRVPSARTP PVLAEKARVP TVPPRPGSSL SGSIENLESE AVFEAKFKRS RESPLSRGLR 1510 1520 1530 1540 1550 1560 LLSRSRSEER GPFRGAEDDG IYRPSPAGTP LELVRRPERS RSVQDLRVAG EPGLVRRLSL 1570 1580 1590 1600 1610 1620 SLSQKLRRTP PGQRHPAWES RSGDGESSEG GSSARASPVL AVRRRLSSTL ERLSSRLQRS 1630 1640 1650 1660 1670 1680 GSSEDSGGAS GRSTPLFGRL RRATSEGESL RRLGVPHNQL GSQTGATTPS AESLGSEASG 1690 1700 1710 1720 1730 1740 TSGSSAPGES RSRHRWGLSR LRKDKGLSQP NLSSSVQEDL GHQYVPSESD FPPVFHIKLK 1750 1760 1770 1780 1790 1800 DQVLLEGEAA TLLCLPAACP APRISWMKDK QSLRSEPSVV IVSCKDGRQL LSIPRAGKRH 1810 1820 1830 1840 1850 1860 AGLYECSATN VLGSITSSCT VAVARIPGKL APPEVPQTYH DTALVVWKPG DGRAPCTYTL 1870 1880 1890 1900 1910 1920 ERRVDGESVW HPVSSGIPDC YYNVTQLPVG VTVRFRVACS NRAGQGPFSN PSEKVFIRGT 1930 1940 1950 1960 1970 1980 PDSPAQPAAA PRDAPVTSGP TRAPPPDSPT SLAPTPALAP PASQASTLSP STSSMSANQA 1990 2000 2010 2020 2030 2040 LSSLKAVGPP PATPPRKHRG LLATQQAEPS PPSIVVTPSE PRSFVPDTGT LTPTSSPQGV 2050 2060 2070 2080 2090 2100 KPAPSSTSLY MVTSFVSAPP APQAPAPEPP PEPTKVTVRS LSPAKEVVSS PTPESTTLRQ 2110 2120 2130 2140 2150 2160 GPPQKPYTFL EEKARGRFGV VRSCRENATG RTFVAKIVPY AAEGKRRVLQ EYEVLRTLHH 2170 2180 2190 2200 2210 2220 ERLMSLHEAY ITPRYLVLIA ESCGNRELLC GLSDRFRYSE DDVATYVVQL LQGLDYLHGH 2230 2240 2250 2260 2270 2280 HVLHLDIKPD NLLLAADNAL KIVDFGSAQP YNPQALKPLG HRTGTLEFMA PEMVKGDPIG 2290 2300 2310 2320 2330 2340 SATDIWGAGV LTYIMLSGYS PFYEPDPQET EARIVGGRFD AFQLYPNTSQ SATLFLRKVL 2350 2360 2370 2380 2390 2400 SVHPWSRPSL QDCLAHPWLQ DAYLMKLRRQ TLTFTTNRLK EFLGEQRRRR AEAATRHKVL LRSYPGSP
	Isoform 4 (SPEG-alpha) [UniParc]. Checksum: 9086EDEF4F90F288	FASTA	2,408	262,899
	10 20 30 40 50 60 MKLSPSQDHD SSDSSSKAPP TFKVSLMDQS VREGQDVIMS IRVQGEPKPV VSWLRNRQPV 70 80 90 100 110 120 RPDQRRFAEE AEGGLCRLRI LAAERGDAGF YTCKAVNEYG ARQCEARLEV RAHPESRSLA 130 140 150 160 170 180 VLAPLQDVDV GAGEMALFEC LVAGPADVEV DWLCRGRLLQ PALLKCKMHF DGRKCKLLLT 190 200 210 220 230 240 SVHEDDSGVY TCKLSTAKDE LTCSARLTVR PSLAPLFTRL LEDVEVLEGR AARLDCKISG 250 260 270 280 290 300 TPPPSVTWTH FGHPVNEGDN LRLRQDGGLH SLHIARVGSE DEGLYEVSAT NTHGQAHCSA 310 320 330 340 350 360 QLYVEEPRTA ASGPSSKLEK MPSIPEEPEH GDLERLSIPD FLRPLQDLEV GLAKEAMLEC 370 380 390 400 410 420 QVTGLPYPTI SWFHNGHRIQ SSDDRRMTQY RDIHRLVFPA VGPQHAGVYK SVIANKLGKA 430 440 450 460 470 480 ACYAHLYVTD VVPGPPDGAP EVVAVTGRMV TLSWNPPRSL DMAIDPDSLT YTVQHQVLGS 490 500 510 520 530 540 DQWTALVTGL REPAWAATGL KKGIQHIFRV LSSSGKSSSK PSAPSEPVQL LEHGPPLEEA 550 560 570 580 590 600 PAVLDKQDIV YVVEGQPACV TVTFNHVEAQ VVWRSCRGAL LEARTGVYEL SQPDDDQYCL 610 620 630 640 650 660 RICRVSRRDL GPLTCSARNR HGTKACSVTL ELAEAPRFES IMEDVEVGPG ETARFAVVVE 670 680 690 700 710 720 GKPLPDIMWY KDEVLLAESN HVSFVYEENE CSLVLLSAGS QDGGVYTCTA RNLAGEVSCK 730 740 750 760 770 780 AELSVLSAQT AMEVEGVGED EEHRGRRLSD YYDIHQEIGR GAFSYLRRVV ERSSGLEFAA 790 800 810 820 830 840 KFIPSQAKPK ASARREARLL ARLQHGCVLY FHEAFERRRG LVIVTELCTE ELLERMARKP 850 860 870 880 890 900 TVCESETRTY MRQVLEGICY LHQSHVLHLD VKPENLLVWD GAGGEEQVRI CDFGNAQELT 910 920 930 940 950 960 PGEPQYCQYG TPEFVAPEIV NQSPVSGVTD IWPVGVVAFL CLTGISPFVG ENDRTTLMNI 970 980 990 1000 1010 1020 RNYNVAFEET TFLSLSREAR GFLIKVLVQD RLRPTAEETL EHPWFKTEAK GAEVSTDHLK 1030 1040 1050 1060 1070 1080 LFLSRRRWQR SQISYKCHLV LRPIPELLRA PPERVWVAMP RRQPPSGGLS SSSDSEEEEL 1090 1100 1110 1120 1130 1140 EELPSVPRPL QPEFSGSRVS LTDIPTEDEA LGTPEAGAAT PMDWQEQERT PSKDQEAPSP 1150 1160 1170 1180 1190 1200 EALPSPGQES PDGPSPRRPE LRRGSSAESA LPRVGSREPG RSLHKAASVE LPQRRSPSPG 1210 1220 1230 1240 1250 1260 ATRLTRGGLG EGEYAQRLQA LRQRLLRGGP EDGKVSGLRG PLLESLGGRA RDPRMARAAS 1270 1280 1290 1300 1310 1320 SEAAPHHQPP PESRGLQKSS SFSQGEAEPR GRHRRAGAPL EIPVARLGAR RLQESPSLSA 1330 1340 1350 1360 1370 1380 LSETQPPSPA RPSVPKLSIT KSPEPSAVTS RDSPQPPEPQ PVPEKVPEPK PEPVRAAKPA 1390 1400 1410 1420 1430 1440 QPPLALQMPT QPLTPYAQIM QSLQLSSPTL SPQDPAVPPS EPKPHAAVFA RVASPPPGVS 1450 1460 1470 1480 1490 1500 EKRVPSARTP PVLAEKARVP TVPPRPGSSL SGSIENLESE AVFEAKFKRS RESPLSRGLR 1510 1520 1530 1540 1550 1560 LLSRSRSEER GPFRGAEDDG IYRPSPAGTP LELVRRPERS RSVQDLRVAG EPGLVRRLSL 1570 1580 1590 1600 1610 1620 SLSQKLRRTP PGQRHPAWES RSGDGESSEG GSSARASPVL AVRRRLSSTL ERLSSRLQRS 1630 1640 1650 1660 1670 1680 GSSEDSGGAS GRSTPLFGRL RRATSEGESL RRLGVPHNQL GSQTGATTPS AESLGSEASG 1690 1700 1710 1720 1730 1740 TSGSSAPGES RSRHRWGLSR LRKDKGLSQP NLSSSVQEDL GHQYVPSESD FPPVFHIKLK 1750 1760 1770 1780 1790 1800 DQVLLEGEAA TLLCLPAACP APRISWMKDK QSLRSEPSVV IVSCKDGRQL LSIPRAGKRH 1810 1820 1830 1840 1850 1860 AGLYECSATN VLGSITSSCT VAVARIPGKL APPEVPQTYH DTALVVWKPG DGRAPCTYTL 1870 1880 1890 1900 1910 1920 ERRVDGESVW HPVSSGIPDC YYNVTQLPVG VTVRFRVACS NRAGQGPFSN PSEKVFIRGT 1930 1940 1950 1960 1970 1980 PDSPAQPAAA PRDAPVTSGP TRAPPPDSPT SLAPTPALAP PASQASTLSP STSSMSANQA 1990 2000 2010 2020 2030 2040 LSSLKAVGPP PATPPRKHRG LLATQQAEPS PPSIVVTPSE PRSFVPDTGT LTPTSSPQGV 2050 2060 2070 2080 2090 2100 KPAPSSTSLY MVTSFVSAPP APQAPAPEPP PEPTKVTVRS LSPAKEVVSS PTPESTTLRQ 2110 2120 2130 2140 2150 2160 GPPQKPYTFL EEKARGRFGV VRSCRENATG RTFVAKIVPY AAEGKRRVLQ EYEVLRTLHH 2170 2180 2190 2200 2210 2220 ERLMSLHEAY ITPRYLVLIA ESCGNRELLC GLSDRFRYSE DDVATYVVQL LQGLDYLHGH 2230 2240 2250 2260 2270 2280 HVLHLDIKPD NLLLAADNAL KIVDFGSAQP YNPQALKPLG HRTGTLEFMA PEMVKGDPIG 2290 2300 2310 2320 2330 2340 SATDIWGAGV LTYIMLSGYS PFYEPDPQET EARIVGGRFD AFQLYPNTSQ SATLFLRKVL 2350 2360 2370 2380 2390 2400 SVHPWSRPSL QDCLAHPWLQ DAYLMKLRRQ TLTFTTNRLK EFLGEQRRRR AEAATRHKVL LRSYPGSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"APEG-1, a novel gene preferentially expressed in aortic smooth muscle cells, is down-regulated by vascular injury." Hsieh C.-M., Yoshizumi M., Endege W.O., Kho C.-J., Jain M.K., Kashiki S., de Los Santos R., Lee W.-S., Perrella M.A., Lee M.-E. J. Biol. Chem. 271:17354-17359(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION. Strain: C57BL/6.
[2]	"Striated muscle preferentially expressed genes alpha and beta are two serine/threonine protein kinases derived from the same gene as the aortic preferentially expressed gene-1." Hsieh C.-M., Fukumoto S., Layne M.D., Maemura K., Charles H., Patel A., Perrella M.A., Lee M.-E. J. Biol. Chem. 275:36966-36973(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), TISSUE SPECIFICITY, PHOSPHORYLATION. Strain: BALB/c.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Spinal ganglion and Urinary bladder.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 723-3262 (ISOFORM 2). Strain: C57BL/6. Tissue: Embryonic brain and Limb.
[5]	"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H. DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2092-3262. Tissue: Brain.
[6]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-481 AND SER-1177, MASS SPECTROMETRY. Tissue: Brain.
[7]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-316, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-439, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U57098 mRNA. Translation: AAC52666.1. AF215896 mRNA. Translation: AAG34791.1. Frameshift. AK035543 mRNA. Translation: BAC29098.1. AK164360 mRNA. Translation: BAE37758.1. BC048698 mRNA. Translation: AAH48698.3. BC062643 mRNA. Translation: AAH62643.1. AK122488 mRNA. Translation: BAC65770.1.
IPI	IPI00331223. IPI00752259. IPI00755319. IPI00762377.
RefSeq	NP_001078839.1. NM_001085370.1. NP_001078840.1. NM_001085371.1. NP_001166948.1. NM_001173477.1.
UniGene	Mm.275397.
3D structure databases
ProteinModelPortal	Q62407.
SMR	Q62407. Positions 871-966.
ModBase	Search...
PTM databases
PhosphoSite	Q62407.
Proteomic databases
PaxDb	Q62407.
PRIDE	Q62407.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000113587; ENSMUSP00000109217; ENSMUSG00000026207. ENSMUST00000113588; ENSMUSP00000109218; ENSMUSG00000026207. ENSMUST00000113589; ENSMUSP00000109219; ENSMUSG00000026207. ENSMUST00000113590; ENSMUSP00000109220; ENSMUSG00000026207. ENSMUST00000122266; ENSMUSP00000113646; ENSMUSG00000026207.
GeneID	11790.
KEGG	mmu:11790.
UCSC	uc007boz.1. mouse. uc007bpa.1. mouse.
Organism-specific databases
CTD	10290.
MGI	MGI:109282. Speg.
Rouge	Search...
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00700000104431.
HOVERGEN	HBG083339.
InParanoid	Q62407.
KO	K08809.
OrthoDB	EOG4J6RPZ.
Gene expression databases
ArrayExpress	Q62407.
Bgee	Q62407.
CleanEx	MM_SPEG.
Genevestigator	Q62407.
GermOnline	ENSMUSG00000026207. Mus musculus.
Family and domain databases
Gene3D	2.60.40.10. 11 hits.
InterPro	IPR003961. Fibronectin_type3. IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR013098. Ig_I-set. IPR003599. Ig_sub. IPR003598. Ig_sub2. IPR011009. Kinase-like_dom. IPR020675. Myosin_light_ch_kinase-rel. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR015726. Ser/Thr_kin_striated_muscle-sp. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
PANTHER	PTHR22964. PTHR22964. 1 hit. PTHR22964:SF9. PTHR22964:SF9. 1 hit.
Pfam	PF07679. I-set. 8 hits. PF00069. Pkinase. 2 hits. [Graphical view]
SMART	SM00060. FN3. 2 hits. SM00409. IG. 4 hits. SM00408. IGc2. 5 hits. SM00220. S_TKc. 2 hits. [Graphical view]
SUPFAM	SSF49265. FN_III-like. 2 hits. SSF56112. Kinase_like. 2 hits.
PROSITE	PS50853. FN3. 2 hits. PS50835. IG_LIKE. 8 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 2 hits. PS00108. PROTEIN_KINASE_ST. 2 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SPEG. mouse.
NextBio	279613.
SOURCE	Search...

Entry information

Entry name

SPEG_MOUSE

Accession

Primary (citable) accession number: Q62407
Secondary accession number(s): Q3TPH8 , Q6P5V1, Q80TF7, Q80ZN0, Q8BZF4, Q9EQJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 16, 2006
Last modified:	April 3, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program