ID   CNGA2_MOUSE             Reviewed;         664 AA.
AC   Q62398; Q80XH6;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   24-JAN-2024, entry version 175.
DE   RecName: Full=Cyclic nucleotide-gated olfactory channel;
DE   AltName: Full=Cyclic nucleotide-gated cation channel 2;
DE   AltName: Full=Cyclic nucleotide-gated channel alpha-2;
DE            Short=CNG channel alpha-2;
DE            Short=CNG-2;
DE            Short=CNG2;
GN   Name=Cnga2; Synonyms=Cncg2, Cncg4;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Heart;
RX   PubMed=8841933; DOI=10.1006/jmcc.1996.0136;
RA   Ruiz M.L., London B., Nadal-Ginard B.;
RT   "Cloning and characterization of an olfactory cyclic nucleotide-gated
RT   channel expressed in mouse heart.";
RL   J. Mol. Cell. Cardiol. 28:1453-1461(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Olfactory epithelium;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Odorant signal transduction is probably mediated by a G-
CC       protein coupled cascade using cAMP as second messenger. The olfactory
CC       channel can be shown to be activated by cyclic nucleotides which leads
CC       to a depolarization of olfactory sensory neurons.
CC   -!- SUBUNIT: Heterotetramer composed of two subunits of CNGA2, one of CNGA4
CC       and one of CNGB1b. The complex forms the cyclic nucleotide-gated (CNG)
CC       channel of olfactory sensory neurons (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- DOMAIN: The C-terminal coiled-coil domain mediates trimerization of
CC       CNGA subunits. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cyclic nucleotide-gated cation channel
CC       (TC 1.A.1.5) family. CNGA2 subfamily. {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-2 is the initiator.
CC       {ECO:0000305}.
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DR   EMBL; U49391; AAC52712.1; -; Genomic_DNA.
DR   EMBL; AK132574; BAE21237.1; -; mRNA.
DR   EMBL; CH466624; EDL26564.1; -; Genomic_DNA.
DR   EMBL; BC048775; AAH48775.1; -; mRNA.
DR   CCDS; CCDS30184.1; -.
DR   RefSeq; NP_031750.2; NM_007724.3.
DR   RefSeq; XP_006527824.1; XM_006527761.1.
DR   AlphaFoldDB; Q62398; -.
DR   SMR; Q62398; -.
DR   STRING; 10090.ENSMUSP00000006020; -.
DR   TCDB; 1.A.1.5.4; the voltage-gated ion channel (vic) superfamily.
DR   GlyCosmos; Q62398; 1 site, No reported glycans.
DR   GlyGen; Q62398; 1 site.
DR   PhosphoSitePlus; Q62398; -.
DR   SwissPalm; Q62398; -.
DR   PaxDb; 10090-ENSMUSP00000006020; -.
DR   ProteomicsDB; 285507; -.
DR   Antibodypedia; 17043; 299 antibodies from 31 providers.
DR   DNASU; 12789; -.
DR   Ensembl; ENSMUST00000006020.8; ENSMUSP00000006020.8; ENSMUSG00000005864.8.
DR   GeneID; 12789; -.
DR   KEGG; mmu:12789; -.
DR   UCSC; uc009tkk.1; mouse.
DR   AGR; MGI:108040; -.
DR   CTD; 1260; -.
DR   MGI; MGI:108040; Cnga2.
DR   VEuPathDB; HostDB:ENSMUSG00000005864; -.
DR   eggNOG; KOG0500; Eukaryota.
DR   GeneTree; ENSGT00940000155374; -.
DR   HOGENOM; CLU_005746_12_0_1; -.
DR   InParanoid; Q62398; -.
DR   OMA; DWYYHWL; -.
DR   OrthoDB; 74296at2759; -.
DR   PhylomeDB; Q62398; -.
DR   TreeFam; TF319048; -.
DR   Reactome; R-MMU-5620916; VxPx cargo-targeting to cilium.
DR   BioGRID-ORCS; 12789; 1 hit in 76 CRISPR screens.
DR   PRO; PR:Q62398; -.
DR   Proteomes; UP000000589; Chromosome X.
DR   RNAct; Q62398; Protein.
DR   Bgee; ENSMUSG00000005864; Expressed in olfactory epithelium and 33 other cell types or tissues.
DR   ExpressionAtlas; Q62398; baseline and differential.
DR   GO; GO:0060170; C:ciliary membrane; IDA:ARUK-UCL.
DR   GO; GO:0017071; C:intracellular cyclic nucleotide activated cation channel complex; ISO:MGI.
DR   GO; GO:0043204; C:perikaryon; ISO:MGI.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR   GO; GO:0030552; F:cAMP binding; IEA:UniProtKB-KW.
DR   GO; GO:0030553; F:cGMP binding; ISO:MGI.
DR   GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR   GO; GO:0005222; F:intracellular cAMP-activated cation channel activity; ISO:MGI.
DR   GO; GO:0005223; F:intracellular cGMP-activated cation channel activity; ISO:MGI.
DR   GO; GO:0005216; F:monoatomic ion channel activity; IMP:MGI.
DR   GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR   GO; GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
DR   GO; GO:0051899; P:membrane depolarization; ISO:MGI.
DR   GO; GO:0098655; P:monoatomic cation transmembrane transport; IBA:GO_Central.
DR   GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR   GO; GO:0007608; P:sensory perception of smell; IMP:MGI.
DR   CDD; cd00038; CAP_ED; 1.
DR   Gene3D; 1.10.287.70; -; 1.
DR   Gene3D; 1.20.5.300; -; 1.
DR   Gene3D; 1.10.287.630; Helix hairpin bin; 1.
DR   Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR   InterPro; IPR032406; CLZ_dom.
DR   InterPro; IPR018488; cNMP-bd_CS.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR018490; cNMP-bd_dom_sf.
DR   InterPro; IPR005821; Ion_trans_dom.
DR   InterPro; IPR003938; K_chnl_volt-dep_EAG/ELK/ERG.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   PANTHER; PTHR45638; CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A; 1.
DR   PANTHER; PTHR45638:SF3; CYCLIC NUCLEOTIDE-GATED OLFACTORY CHANNEL; 1.
DR   Pfam; PF16526; CLZ; 1.
DR   Pfam; PF00027; cNMP_binding; 1.
DR   Pfam; PF00520; Ion_trans; 1.
DR   PRINTS; PR01463; EAGCHANLFMLY.
DR   SMART; SM00100; cNMP; 1.
DR   SUPFAM; SSF51206; cAMP-binding domain-like; 1.
DR   SUPFAM; SSF81324; Voltage-gated potassium channels; 1.
DR   PROSITE; PS00888; CNMP_BINDING_1; 1.
DR   PROSITE; PS00889; CNMP_BINDING_2; 1.
DR   PROSITE; PS50042; CNMP_BINDING_3; 1.
DR   Genevisible; Q62398; MM.
PE   2: Evidence at transcript level;
KW   Calmodulin-binding; cAMP; cAMP-binding; Coiled coil; Glycoprotein;
KW   Ion channel; Ion transport; Ligand-gated ion channel; Membrane;
KW   Nucleotide-binding; Olfaction; Reference proteome; Sensory transduction;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..664
FT                   /note="Cyclic nucleotide-gated olfactory channel"
FT                   /id="PRO_0000219313"
FT   TOPO_DOM        1..142
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical; Name=H1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        164..175
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..194
FT                   /note="Helical; Name=H2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        195..218
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        219..238
FT                   /note="Helical; Name=H3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        239..276
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        277..299
FT                   /note="Helical; Name=H4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        300..351
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        352..371
FT                   /note="Helical; Name=H5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        372..455
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        456..476
FT                   /note="Helical; Name=H6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        477..664
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          599..642
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        1..22
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        24..40
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         464..586
FT                   /ligand="3',5'-cyclic AMP"
FT                   /ligand_id="ChEBI:CHEBI:58165"
FT                   /evidence="ECO:0000250"
FT   BINDING         523
FT                   /ligand="3',5'-cyclic AMP"
FT                   /ligand_id="ChEBI:CHEBI:58165"
FT                   /evidence="ECO:0000255"
FT   BINDING         538
FT                   /ligand="3',5'-cyclic AMP"
FT                   /ligand_id="ChEBI:CHEBI:58165"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        381
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        110
FT                   /note="T -> P (in Ref. 1; AAC52712)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        355
FT                   /note="V -> F (in Ref. 1; AAC52712)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        540..541
FT                   /note="AN -> GT (in Ref. 1; AAC52712)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   664 AA;  76193 MW;  34F8DF3B372C0D9C CRC64;
     MMTEKSNGVK SSPANNHNHH PPPSIKANGK DDHRAGSRPQ SVAADDDTSS ELQRLAEMDT
     PRRGRGGFRR IVRLVGIIRD WANKNFREEE PRPDSFLERF RGPELQTVTT HQGDGKGDKD
     GEGKGTKKKF ELFVLDPAGD WYYRWLFVIA MPVLYNWCLL VARACFSDLQ RNYFVVWLVL
     DYFSDTVYIA DLIIRLRTGF LEQGLLVKDP KKLRDNYIHT LQFKLDVASI IPTDLIYFAV
     GIHSPEVRFN RLLHFARMFE FFDRTETRTS YPNIFRISNL VLYILVIIHW NACIYYAISK
     SIGFGVDTWV YPNITDPEYG YLAREYIYCL YWSTLTLTTI GETPPPVKDE EYLFVIFDFL
     IGVLIFATIV GNVGSMISNM NATRAEFQAK IDAVKHYMQF RKVSKDMEAK VIKWFDYLWT
     NKKTVDEREV LKNLPAKLRA EIAINVHLST LKKVRIFQDC EAGLLVELVL KLRPQVFSPG
     DYICRKGDIG KEMYIIKEGK LAVVADDGVT QYALLSAGSC FGEISILNIK GSKMGNRRTA
     NIRSLGYSDL FCLSKDDLME AVTEYPDAKK VLEERGREIL MKEGLLDENE VAASMEVDVQ
     EKLEQLETNM ETLYTRFARL LAEYTGAQQK LKQRITVLET KMKQNHEDDY LSDGINTPEP
     AVAE
//