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Q62384

- ZPR1_MOUSE

UniProt

Q62384 - ZPR1_MOUSE

Protein

Zinc finger protein ZPR1

Gene

Zpr1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts as a signaling molecule that communicates proliferative growth signals from the cytoplasm to the nucleus. Plays a role for the localization and accumulation of the survival motor neuron protein SMN1 in sub-nuclear bodies, including gems and Cajal bodies. Induces neuron differentiation and stimulates axonal growth and formation of growth cone in spinal cord motor neurons. Plays a role in the splicing of cellular pre-mRNAs. May be involved in H2O(2)-induced neuronal cell death.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri51 – 8333C4-type 1Add
    BLAST
    Zinc fingeri259 – 29133C4-type 2Add
    BLAST

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. receptor tyrosine kinase binding Source: UniProtKB
    3. translation initiation factor binding Source: UniProtKB
    4. zinc ion binding Source: MGI

    GO - Biological processi

    1. apoptotic process involved in development Source: UniProtKB
    2. axon development Source: UniProtKB
    3. Cajal body organization Source: UniProtKB
    4. cellular response to epidermal growth factor stimulus Source: UniProtKB
    5. DNA endoreduplication Source: UniProtKB
    6. inner cell mass cell proliferation Source: UniProtKB
    7. microtubule cytoskeleton organization Source: UniProtKB
    8. mRNA processing Source: UniProtKB-KW
    9. negative regulation of motor neuron apoptotic process Source: UniProtKB
    10. positive regulation of gene expression Source: UniProtKB
    11. positive regulation of growth Source: UniProtKB
    12. positive regulation of protein import into nucleus Source: UniProtKB
    13. positive regulation of RNA splicing Source: UniProtKB
    14. positive regulation of transcription involved in G1/S transition of mitotic cell cycle Source: UniProtKB
    15. pre-mRNA catabolic process Source: UniProtKB
    16. regulation of myelination Source: UniProtKB
    17. RNA splicing Source: UniProtKB-KW
    18. spinal cord development Source: UniProtKB
    19. trophectodermal cell proliferation Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, mRNA processing, mRNA splicing

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Zinc finger protein ZPR1
    Alternative name(s):
    Zinc finger protein 259
    Gene namesi
    Name:Zpr1
    Synonyms:Zfp259, Znf259
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1330262. Zpr1.

    Subcellular locationi

    Nucleus. Cytoplasm. Nucleusnucleolus. Cytoplasmperinuclear region By similarity. Nucleusgem. NucleusCajal body. Cell projectionaxon. Cell projectiongrowth cone
    Note: Localized predominantly in the cytoplasm in serum-starved cells growth arrested in G0 of the mitotic cell cycle. Localized both in the nucleus and cytoplasm at the G1 phase of the mitotic cell cycle. Accumulates in the subnuclear bodies during progression into the S phase of the mitotic cell cycle. Diffusely localized throughout the cell during mitosis. Colocalized with NPAT and SMN1 in nuclear bodies including gems (Gemini of coiled bodies) and Cajal bodies in a cell cycle-dependent manner. Colocalized with EGFR in the cytoplasm of quiescent cells. Translocates from the cytoplasm to the nucleus in a epidermal growth factor (EGF)-dependent manner By similarity. Translocates together with EEF1A1 from the cytoplasm to the nucleolus after treatment with mitogens. Colocalized with SMN1 in Gemini of coiled bodies (gems), Cajal bodies, axon and growth cones of neurons.By similarity

    GO - Cellular componenti

    1. axon Source: UniProtKB
    2. Cajal body Source: UniProtKB
    3. cytoplasm Source: MGI
    4. Gemini of coiled bodies Source: UniProtKB
    5. growth cone Source: UniProtKB
    6. neuronal cell body Source: UniProtKB
    7. nucleolus Source: UniProtKB
    8. nucleoplasm Source: UniProtKB
    9. nucleus Source: MGI
    10. perikaryon Source: UniProtKB
    11. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    12. SMN complex Source: UniProtKB

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Nucleus

    Pathology & Biotechi

    Involvement in diseasei

    May contribute to the severity of spinal muscular atrophy by increasing spinal motor neurons degeneration.1 Publication

    Disruption phenotypei

    Die during early embryonic development. Embryos show growth delay, failed to form normal trophectoderm and to expand the inner cell mass.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 459459Zinc finger protein ZPR1PRO_0000119037Add
    BLAST

    Proteomic databases

    MaxQBiQ62384.
    PaxDbiQ62384.
    PRIDEiQ62384.

    PTM databases

    PhosphoSiteiQ62384.

    Expressioni

    Tissue specificityi

    Expressed in brain. Expressed in the spinal cord motor neurons (at protein level). Expressed in spleen, liver, muscle, kidney and testis. Expressed in the frontal cortex, cornus ammonis, dentate gyrus of the hippocampus and in Purkinje cells of the cerebellum.3 Publications

    Inductioni

    Up-regulated by high fat diet.1 Publication

    Gene expression databases

    ArrayExpressiQ62384.
    BgeeiQ62384.
    CleanExiMM_ZFP259.
    GenevestigatoriQ62384.

    Interactioni

    Subunit structurei

    Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Interacts (via C-terminal region) with SMN1 (via C-terminal region); the interaction occurs after treatment with serum By similarity. Interacts with elongation factor 1-alpha EEF1A1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner. Interacts (via zinc fingers) with EGFR (via C-terminal cytoplasmic kinase domain); the interaction is negatively regulated in response to epidermal growth factor (EGF) stimulation and EGFR kinase activity. May also bind to the PDGFR receptor.By similarity5 Publications

    Protein-protein interaction databases

    BioGridi204651. 1 interaction.
    DIPiDIP-46465N.

    Structurei

    Secondary structure

    1
    459
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi48 – 503
    Turni52 – 543
    Beta strandi55 – 6713
    Turni68 – 703
    Beta strandi71 – 799
    Turni81 – 833
    Beta strandi86 – 9611
    Beta strandi98 – 10710
    Helixi110 – 1134
    Beta strandi116 – 1194
    Beta strandi124 – 1274
    Helixi128 – 1303
    Beta strandi132 – 1343
    Beta strandi142 – 1454
    Helixi146 – 15813
    Helixi161 – 1677
    Helixi169 – 18416
    Helixi185 – 1873
    Beta strandi192 – 1987
    Turni208 – 2114
    Beta strandi217 – 2226
    Helixi226 – 2316
    Beta strandi252 – 2576
    Turni260 – 2623
    Beta strandi268 – 2736
    Beta strandi281 – 2877
    Beta strandi289 – 2913
    Beta strandi294 – 3007
    Beta strandi302 – 3043
    Beta strandi308 – 3158
    Helixi318 – 3225
    Beta strandi324 – 3274
    Beta strandi332 – 3354
    Helixi336 – 3383
    Beta strandi340 – 3423
    Turni344 – 3474
    Beta strandi349 – 3535
    Helixi354 – 36613
    Beta strandi372 – 3743
    Helixi378 – 3803
    Helixi382 – 39514
    Beta strandi401 – 4077
    Turni417 – 4204
    Beta strandi426 – 4316
    Turni437 – 4393

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QKDX-ray2.00A47-440[»]
    ProteinModelPortaliQ62384.
    SMRiQ62384. Positions 47-440.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ62384.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ZPR1 family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri51 – 8333C4-type 1Add
    BLAST
    Zinc fingeri259 – 29133C4-type 2Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1779.
    GeneTreeiENSGT00390000005306.
    HOVERGENiHBG002608.
    InParanoidiQ62384.
    KOiK06874.
    OMAiFIMNDPA.
    OrthoDBiEOG7GQXVP.
    TreeFamiTF313084.

    Family and domain databases

    InterProiIPR004457. Znf_ZPR1.
    [Graphical view]
    PfamiPF03367. zf-ZPR1. 2 hits.
    [Graphical view]
    SMARTiSM00709. Zpr1. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00310. ZPR1_znf. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q62384-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSASGAVQPG HPGAAVGPSP AAAASPATGP LFRPLSAEDE EQQPTEIESL    50
    CMNCYRNGTT RLLLTKIPFF REIIVSSFSC EHCGWNNTEI QSAGRIQDQG 100
    VRYTLTVRSQ EDMNREVVKT DSATTRIPEL DFEIPAFSQK GALTTVEGLI 150
    SRAISGLEQD QPTRRAVEGA IAERIDEFIG KLKDLKQMAS PFTLVIDDPS 200
    GNSFVENPHA PQKDNALVIT YYDRTPQQAE MLGLQAEAPE EKAEEEDLRN 250
    EVLQFNTNCP ECNAPAQTNM KLVQIPHFKE VIIMATNCEN CGHRTNEVKS 300
    GGAVEPLGTR ITLHITDPSD MTRDLLKSET CSVEIPELEF ELGMAVLGGK 350
    FTTLEGLLKD IRELVTKNPF TLGDSSNPDQ SEKLQEFSQK LGQIIEGKMK 400
    AHFIMNDPAG NSYLQNVYAP EDDPEMKVER YKRTFDQNEE LGLNDMKTEG 450
    YEAGLAPQR 459
    Length:459
    Mass (Da):50,715
    Last modified:November 1, 1996 - v1
    Checksum:i771D38C7B806044F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U41287 mRNA. Translation: AAC52662.1.
    BC021397 mRNA. Translation: AAH21397.1.
    CCDSiCCDS23144.1.
    RefSeqiNP_035882.1. NM_011752.2.
    UniGeneiMm.17519.

    Genome annotation databases

    EnsembliENSMUST00000156440; ENSMUSP00000117725; ENSMUSG00000032078.
    GeneIDi22687.
    KEGGimmu:22687.
    UCSCiuc009phh.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U41287 mRNA. Translation: AAC52662.1 .
    BC021397 mRNA. Translation: AAH21397.1 .
    CCDSi CCDS23144.1.
    RefSeqi NP_035882.1. NM_011752.2.
    UniGenei Mm.17519.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2QKD X-ray 2.00 A 47-440 [» ]
    ProteinModelPortali Q62384.
    SMRi Q62384. Positions 47-440.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204651. 1 interaction.
    DIPi DIP-46465N.

    PTM databases

    PhosphoSitei Q62384.

    Proteomic databases

    MaxQBi Q62384.
    PaxDbi Q62384.
    PRIDEi Q62384.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000156440 ; ENSMUSP00000117725 ; ENSMUSG00000032078 .
    GeneIDi 22687.
    KEGGi mmu:22687.
    UCSCi uc009phh.1. mouse.

    Organism-specific databases

    CTDi 8882.
    MGIi MGI:1330262. Zpr1.

    Phylogenomic databases

    eggNOGi COG1779.
    GeneTreei ENSGT00390000005306.
    HOVERGENi HBG002608.
    InParanoidi Q62384.
    KOi K06874.
    OMAi FIMNDPA.
    OrthoDBi EOG7GQXVP.
    TreeFami TF313084.

    Miscellaneous databases

    EvolutionaryTracei Q62384.
    NextBioi 303121.
    PROi Q62384.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q62384.
    Bgeei Q62384.
    CleanExi MM_ZFP259.
    Genevestigatori Q62384.

    Family and domain databases

    InterProi IPR004457. Znf_ZPR1.
    [Graphical view ]
    Pfami PF03367. zf-ZPR1. 2 hits.
    [Graphical view ]
    SMARTi SM00709. Zpr1. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00310. ZPR1_znf. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Binding of zinc finger protein ZPR1 to the epidermal growth factor receptor."
      Galcheva-Gargova Z., Konstantinov K.N., Wu I.-H., Klier F.G., Barrett T., Davis R.J.
      Science 272:1797-1802(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH EGFR, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells."
      Gangwani L., Mikrut M., Galcheva-Gargova Z., Davis R.J.
      J. Cell Biol. 143:1471-1484(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH EEF1A1 AND EGFR, SUBCELLULAR LOCATION.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary gland.
    4. "The cytoplasmic zinc finger protein ZPR1 accumulates in the nucleolus of proliferating cells."
      Galcheva-Gargova Z., Gangwani L., Konstantinov K.N., Mikrut M., Theroux S.J., Enoch T., Davis R.J.
      Mol. Biol. Cell 9:2963-2971(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "ZPR1 is essential for survival and is required for localization of the survival motor neurons (SMN) protein to Cajal bodies."
      Gangwani L., Flavell R.A., Davis R.J.
      Mol. Cell. Biol. 25:2744-2756(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
    6. "Expression of zinc finger protein ZPR1 mRNA in brain is up-regulated in mice fed a high-fat diet."
      Nogusa Y., Yanaka N., Sumiyoshi N., Takeda K., Kato N.
      Int. J. Mol. Med. 17:491-496(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, TISSUE SPECIFICITY.
    7. Cited for: FUNCTION, INVOLVEMENT IN NEURODEGENERATION, TISSUE SPECIFICITY.
    8. "Generation of a zinc finger protein ZPR1 mutant that constitutively interacted with translation elongation factor 1alpha."
      Yanaka N., Kaseda Y., Tanaka A., Nogusa Y., Sumiyoshi N., Kato N.
      Biosci. Biotechnol. Biochem. 73:2809-2811(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EEF1A1.
    9. "The zinc finger protein ZPR1 is a potential modifier of spinal muscular atrophy."
      Ahmad S., Wang Y., Shaik G.M., Burghes A.H., Gangwani L.
      Hum. Mol. Genet. 21:2745-2758(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH SMN1, INVOLVEMENT IN NEURODEGENERATION, SUBCELLULAR LOCATION.
    10. "Structural insights into the interaction of the evolutionarily conserved ZPR1 domain tandem with eukaryotic EF1A, receptors, and SMN complexes."
      Mishra A.K., Gangwani L., Davis R.J., Lambright D.G.
      Proc. Natl. Acad. Sci. U.S.A. 104:13930-13935(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 47-440, INTERACTION WITH EF1A.

    Entry informationi

    Entry nameiZPR1_MOUSE
    AccessioniPrimary (citable) accession number: Q62384
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3