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Q62384

- ZPR1_MOUSE

UniProt

Q62384 - ZPR1_MOUSE

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Protein

Zinc finger protein ZPR1

Gene

Zpr1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a signaling molecule that communicates proliferative growth signals from the cytoplasm to the nucleus. Plays a role for the localization and accumulation of the survival motor neuron protein SMN1 in sub-nuclear bodies, including gems and Cajal bodies. Induces neuron differentiation and stimulates axonal growth and formation of growth cone in spinal cord motor neurons. Plays a role in the splicing of cellular pre-mRNAs. May be involved in H2O(2)-induced neuronal cell death.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri51 – 8333C4-type 1Add
BLAST
Zinc fingeri259 – 29133C4-type 2Add
BLAST

GO - Molecular functioni

  1. receptor tyrosine kinase binding Source: UniProtKB
  2. translation initiation factor binding Source: UniProtKB
  3. zinc ion binding Source: MGI

GO - Biological processi

  1. apoptotic process involved in development Source: UniProtKB
  2. axon development Source: UniProtKB
  3. Cajal body organization Source: UniProtKB
  4. cellular response to epidermal growth factor stimulus Source: UniProtKB
  5. DNA endoreduplication Source: UniProtKB
  6. inner cell mass cell proliferation Source: UniProtKB
  7. microtubule cytoskeleton organization Source: UniProtKB
  8. mRNA processing Source: UniProtKB-KW
  9. negative regulation of motor neuron apoptotic process Source: UniProtKB
  10. positive regulation of gene expression Source: UniProtKB
  11. positive regulation of growth Source: UniProtKB
  12. positive regulation of protein import into nucleus Source: UniProtKB
  13. positive regulation of RNA splicing Source: UniProtKB
  14. positive regulation of transcription involved in G1/S transition of mitotic cell cycle Source: UniProtKB
  15. pre-mRNA catabolic process Source: UniProtKB
  16. regulation of myelination Source: UniProtKB
  17. RNA splicing Source: UniProtKB-KW
  18. spinal cord development Source: UniProtKB
  19. trophectodermal cell proliferation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein ZPR1
Alternative name(s):
Zinc finger protein 259
Gene namesi
Name:Zpr1
Synonyms:Zfp259, Znf259
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1330262. Zpr1.

Subcellular locationi

Nucleus. Cytoplasm. Nucleusnucleolus. Cytoplasmperinuclear region By similarity. Nucleusgem. NucleusCajal body. Cell projectionaxon. Cell projectiongrowth cone
Note: Localized predominantly in the cytoplasm in serum-starved cells growth arrested in G0 of the mitotic cell cycle. Localized both in the nucleus and cytoplasm at the G1 phase of the mitotic cell cycle. Accumulates in the subnuclear bodies during progression into the S phase of the mitotic cell cycle. Diffusely localized throughout the cell during mitosis. Colocalized with NPAT and SMN1 in nuclear bodies including gems (Gemini of coiled bodies) and Cajal bodies in a cell cycle-dependent manner. Colocalized with EGFR in the cytoplasm of quiescent cells. Translocates from the cytoplasm to the nucleus in a epidermal growth factor (EGF)-dependent manner By similarity. Translocates together with EEF1A1 from the cytoplasm to the nucleolus after treatment with mitogens. Colocalized with SMN1 in Gemini of coiled bodies (gems), Cajal bodies, axon and growth cones of neurons.By similarity

GO - Cellular componenti

  1. axon Source: UniProtKB
  2. Cajal body Source: UniProtKB
  3. cytoplasm Source: MGI
  4. Gemini of coiled bodies Source: UniProtKB
  5. growth cone Source: UniProtKB
  6. neuronal cell body Source: UniProtKB
  7. nucleolus Source: UniProtKB
  8. nucleoplasm Source: UniProtKB
  9. nucleus Source: MGI
  10. perikaryon Source: UniProtKB
  11. SMN complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

May contribute to the severity of spinal muscular atrophy by increasing spinal motor neurons degeneration.1 Publication

Disruption phenotypei

Die during early embryonic development. Embryos show growth delay, failed to form normal trophectoderm and to expand the inner cell mass.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459Zinc finger protein ZPR1PRO_0000119037Add
BLAST

Proteomic databases

MaxQBiQ62384.
PaxDbiQ62384.
PRIDEiQ62384.

PTM databases

PhosphoSiteiQ62384.

Expressioni

Tissue specificityi

Expressed in brain. Expressed in the spinal cord motor neurons (at protein level). Expressed in spleen, liver, muscle, kidney and testis. Expressed in the frontal cortex, cornus ammonis, dentate gyrus of the hippocampus and in Purkinje cells of the cerebellum.3 Publications

Inductioni

Up-regulated by high fat diet.1 Publication

Gene expression databases

BgeeiQ62384.
CleanExiMM_ZFP259.
ExpressionAtlasiQ62384. baseline and differential.
GenevestigatoriQ62384.

Interactioni

Subunit structurei

Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Interacts (via C-terminal region) with SMN1 (via C-terminal region); the interaction occurs after treatment with serum By similarity. Interacts with elongation factor 1-alpha EEF1A1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner. Interacts (via zinc fingers) with EGFR (via C-terminal cytoplasmic kinase domain); the interaction is negatively regulated in response to epidermal growth factor (EGF) stimulation and EGFR kinase activity. May also bind to the PDGFR receptor.By similarity5 Publications

Protein-protein interaction databases

BioGridi204651. 1 interaction.
DIPiDIP-46465N.

Structurei

Secondary structure

1
459
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi48 – 503
Turni52 – 543
Beta strandi55 – 6713
Turni68 – 703
Beta strandi71 – 799
Turni81 – 833
Beta strandi86 – 9611
Beta strandi98 – 10710
Helixi110 – 1134
Beta strandi116 – 1194
Beta strandi124 – 1274
Helixi128 – 1303
Beta strandi132 – 1343
Beta strandi142 – 1454
Helixi146 – 15813
Helixi161 – 1677
Helixi169 – 18416
Helixi185 – 1873
Beta strandi192 – 1987
Turni208 – 2114
Beta strandi217 – 2226
Helixi226 – 2316
Beta strandi252 – 2576
Turni260 – 2623
Beta strandi268 – 2736
Beta strandi281 – 2877
Beta strandi289 – 2913
Beta strandi294 – 3007
Beta strandi302 – 3043
Beta strandi308 – 3158
Helixi318 – 3225
Beta strandi324 – 3274
Beta strandi332 – 3354
Helixi336 – 3383
Beta strandi340 – 3423
Turni344 – 3474
Beta strandi349 – 3535
Helixi354 – 36613
Beta strandi372 – 3743
Helixi378 – 3803
Helixi382 – 39514
Beta strandi401 – 4077
Turni417 – 4204
Beta strandi426 – 4316
Turni437 – 4393

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QKDX-ray2.00A47-440[»]
ProteinModelPortaliQ62384.
SMRiQ62384. Positions 47-440.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62384.

Family & Domainsi

Sequence similaritiesi

Belongs to the ZPR1 family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri51 – 8333C4-type 1Add
BLAST
Zinc fingeri259 – 29133C4-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG1779.
GeneTreeiENSGT00390000005306.
HOVERGENiHBG002608.
InParanoidiQ62384.
KOiK06874.
OMAiFIMNDPA.
OrthoDBiEOG7GQXVP.
TreeFamiTF313084.

Family and domain databases

InterProiIPR004457. Znf_ZPR1.
[Graphical view]
PfamiPF03367. zf-ZPR1. 2 hits.
[Graphical view]
SMARTiSM00709. Zpr1. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00310. ZPR1_znf. 2 hits.

Sequencei

Sequence statusi: Complete.

Q62384-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSASGAVQPG HPGAAVGPSP AAAASPATGP LFRPLSAEDE EQQPTEIESL
60 70 80 90 100
CMNCYRNGTT RLLLTKIPFF REIIVSSFSC EHCGWNNTEI QSAGRIQDQG
110 120 130 140 150
VRYTLTVRSQ EDMNREVVKT DSATTRIPEL DFEIPAFSQK GALTTVEGLI
160 170 180 190 200
SRAISGLEQD QPTRRAVEGA IAERIDEFIG KLKDLKQMAS PFTLVIDDPS
210 220 230 240 250
GNSFVENPHA PQKDNALVIT YYDRTPQQAE MLGLQAEAPE EKAEEEDLRN
260 270 280 290 300
EVLQFNTNCP ECNAPAQTNM KLVQIPHFKE VIIMATNCEN CGHRTNEVKS
310 320 330 340 350
GGAVEPLGTR ITLHITDPSD MTRDLLKSET CSVEIPELEF ELGMAVLGGK
360 370 380 390 400
FTTLEGLLKD IRELVTKNPF TLGDSSNPDQ SEKLQEFSQK LGQIIEGKMK
410 420 430 440 450
AHFIMNDPAG NSYLQNVYAP EDDPEMKVER YKRTFDQNEE LGLNDMKTEG

YEAGLAPQR
Length:459
Mass (Da):50,715
Last modified:November 1, 1996 - v1
Checksum:i771D38C7B806044F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U41287 mRNA. Translation: AAC52662.1.
BC021397 mRNA. Translation: AAH21397.1.
CCDSiCCDS23144.1.
RefSeqiNP_035882.1. NM_011752.2.
UniGeneiMm.17519.

Genome annotation databases

EnsembliENSMUST00000156440; ENSMUSP00000117725; ENSMUSG00000032078.
GeneIDi22687.
KEGGimmu:22687.
UCSCiuc009phh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U41287 mRNA. Translation: AAC52662.1 .
BC021397 mRNA. Translation: AAH21397.1 .
CCDSi CCDS23144.1.
RefSeqi NP_035882.1. NM_011752.2.
UniGenei Mm.17519.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2QKD X-ray 2.00 A 47-440 [» ]
ProteinModelPortali Q62384.
SMRi Q62384. Positions 47-440.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204651. 1 interaction.
DIPi DIP-46465N.

PTM databases

PhosphoSitei Q62384.

Proteomic databases

MaxQBi Q62384.
PaxDbi Q62384.
PRIDEi Q62384.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000156440 ; ENSMUSP00000117725 ; ENSMUSG00000032078 .
GeneIDi 22687.
KEGGi mmu:22687.
UCSCi uc009phh.1. mouse.

Organism-specific databases

CTDi 8882.
MGIi MGI:1330262. Zpr1.

Phylogenomic databases

eggNOGi COG1779.
GeneTreei ENSGT00390000005306.
HOVERGENi HBG002608.
InParanoidi Q62384.
KOi K06874.
OMAi FIMNDPA.
OrthoDBi EOG7GQXVP.
TreeFami TF313084.

Miscellaneous databases

EvolutionaryTracei Q62384.
NextBioi 303121.
PROi Q62384.
SOURCEi Search...

Gene expression databases

Bgeei Q62384.
CleanExi MM_ZFP259.
ExpressionAtlasi Q62384. baseline and differential.
Genevestigatori Q62384.

Family and domain databases

InterProi IPR004457. Znf_ZPR1.
[Graphical view ]
Pfami PF03367. zf-ZPR1. 2 hits.
[Graphical view ]
SMARTi SM00709. Zpr1. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00310. ZPR1_znf. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Binding of zinc finger protein ZPR1 to the epidermal growth factor receptor."
    Galcheva-Gargova Z., Konstantinov K.N., Wu I.-H., Klier F.G., Barrett T., Davis R.J.
    Science 272:1797-1802(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH EGFR, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells."
    Gangwani L., Mikrut M., Galcheva-Gargova Z., Davis R.J.
    J. Cell Biol. 143:1471-1484(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH EEF1A1 AND EGFR, SUBCELLULAR LOCATION.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "The cytoplasmic zinc finger protein ZPR1 accumulates in the nucleolus of proliferating cells."
    Galcheva-Gargova Z., Gangwani L., Konstantinov K.N., Mikrut M., Theroux S.J., Enoch T., Davis R.J.
    Mol. Biol. Cell 9:2963-2971(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "ZPR1 is essential for survival and is required for localization of the survival motor neurons (SMN) protein to Cajal bodies."
    Gangwani L., Flavell R.A., Davis R.J.
    Mol. Cell. Biol. 25:2744-2756(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
  6. "Expression of zinc finger protein ZPR1 mRNA in brain is up-regulated in mice fed a high-fat diet."
    Nogusa Y., Yanaka N., Sumiyoshi N., Takeda K., Kato N.
    Int. J. Mol. Med. 17:491-496(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, TISSUE SPECIFICITY.
  7. Cited for: FUNCTION, INVOLVEMENT IN NEURODEGENERATION, TISSUE SPECIFICITY.
  8. "Generation of a zinc finger protein ZPR1 mutant that constitutively interacted with translation elongation factor 1alpha."
    Yanaka N., Kaseda Y., Tanaka A., Nogusa Y., Sumiyoshi N., Kato N.
    Biosci. Biotechnol. Biochem. 73:2809-2811(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EEF1A1.
  9. "The zinc finger protein ZPR1 is a potential modifier of spinal muscular atrophy."
    Ahmad S., Wang Y., Shaik G.M., Burghes A.H., Gangwani L.
    Hum. Mol. Genet. 21:2745-2758(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SMN1, INVOLVEMENT IN NEURODEGENERATION, SUBCELLULAR LOCATION.
  10. "Structural insights into the interaction of the evolutionarily conserved ZPR1 domain tandem with eukaryotic EF1A, receptors, and SMN complexes."
    Mishra A.K., Gangwani L., Davis R.J., Lambright D.G.
    Proc. Natl. Acad. Sci. U.S.A. 104:13930-13935(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 47-440, INTERACTION WITH EF1A.

Entry informationi

Entry nameiZPR1_MOUSE
AccessioniPrimary (citable) accession number: Q62384
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3