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Protein

Transcription elongation factor SPT6

Gene

Supt6h

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.4 Publications

GO - Molecular functioni

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • mRNA transport Source: UniProtKB-KW
  • negative regulation of histone H3-K27 methylation Source: UniProtKB
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: UniProtKB
  • regulation of isotype switching Source: UniProtKB
  • regulation of mRNA export from nucleus Source: UniProtKB
  • regulation of mRNA processing Source: UniProtKB
  • regulation of muscle cell differentiation Source: UniProtKB
  • RNA splicing Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transcription, Transcription regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor SPT6
Gene namesi
Name:Supt6h
Synonyms:Kiaa0162, Supt6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107726. Supt6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1358 – 13581R → K: Loss of binding to POLR2A. Retains the ability to support transcription elongation but the resulting transcripts contain splicing defects and accumulate to high levels within the nucleus. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 17261725Transcription elongation factor SPT6PRO_0000072172Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei7 – 71PhosphoserineBy similarity
Modified residuei12 – 121PhosphoserineBy similarity
Modified residuei73 – 731PhosphoserineCombined sources
Modified residuei78 – 781PhosphoserineCombined sources
Modified residuei91 – 911PhosphoserineCombined sources
Modified residuei125 – 1251PhosphoserineCombined sources
Modified residuei267 – 2671PhosphoserineBy similarity
Modified residuei743 – 7431N6-acetyllysineBy similarity
Modified residuei1515 – 15151PhosphotyrosineBy similarity
Modified residuei1523 – 15231PhosphothreonineBy similarity
Modified residuei1526 – 15261PhosphoserineBy similarity
Modified residuei1532 – 15321PhosphothreonineCombined sources
Modified residuei1535 – 15351PhosphoserineCombined sources
Modified residuei1539 – 15391PhosphothreonineCombined sources
Modified residuei1676 – 16761N6-acetyllysineBy similarity
Modified residuei1697 – 16971PhosphothreonineBy similarity
Modified residuei1701 – 17011PhosphoserineCombined sources
Modified residuei1703 – 17031PhosphoserineCombined sources
Modified residuei1709 – 17091PhosphothreonineCombined sources
Modified residuei1718 – 17181PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ62383.
MaxQBiQ62383.
PaxDbiQ62383.
PeptideAtlasiQ62383.
PRIDEiQ62383.

PTM databases

iPTMnetiQ62383.
PhosphoSiteiQ62383.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSMUSG00000002052.
CleanExiMM_SUPT6H.
GenevisibleiQ62383. MM.

Interactioni

Subunit structurei

Interacts with RNA polymerase II and the DRB sensitivity-inducing factor complex (DSIF complex), which is composed of SUPT5H and SUPT4H1 or SUPT4H2 (By similarity). Interacts with PAAF1 (By similarity). Interacts with histone H2B and H3 (By similarity). Interacts (via SH2 domain) with POLR2A phosphorylated at 'Ser-2'. Interacts (via SH2 domain) with SETD1A. Interacts with IWS1, KDM6A and AICDA.By similarity5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203576. 5 interactions.
IntActiQ62383. 4 interactions.
MINTiMINT-4136149.
STRINGi10090.ENSMUSP00000002121.

Structurei

3D structure databases

ProteinModelPortaliQ62383.
SMRiQ62383. Positions 270-1176, 1326-1512.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1213 – 128270S1 motifPROSITE-ProRule annotationAdd
BLAST
Domaini1325 – 1431107SH2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 916915Interaction with PAAF1By similarityAdd
BLAST
Regioni2 – 485484Interaction with IWS1Add
BLAST
Regioni317 – 1300984Interaction with KDM6AAdd
BLAST
Regioni1633 – 172694Interaction with histone H2B and H3By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili3 – 5149Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 250245Asp/Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the SPT6 family.Curated
Contains 1 S1 motif domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1856. Eukaryota.
COG2183. LUCA.
GeneTreeiENSGT00510000047446.
HOGENOMiHOG000001562.
HOVERGENiHBG093994.
InParanoidiQ62383.
KOiK11292.
OMAiDLKFRAF.
OrthoDBiEOG091G00SW.
PhylomeDBiQ62383.
TreeFamiTF105956.

Family and domain databases

Gene3Di1.10.150.310. 1 hit.
1.10.3500.10. 2 hits.
2.40.50.140. 1 hit.
3.30.420.140. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR012337. RNaseH-like_dom.
IPR010994. RuvA_2-like.
IPR022967. S1_dom.
IPR003029. S1_domain.
IPR000980. SH2.
IPR028083. Spt6_acidic_N_dom.
IPR027999. Spt6_dom.
IPR032706. Spt6_HHH.
IPR028088. Spt6_HTH_DNA-bd_dom.
IPR028231. Spt6_YqgF.
IPR023323. Tex-like_dom.
IPR023097. Tex_RuvX-like_dom.
IPR017072. TF_Spt6.
IPR006641. YqgF/RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10145:SF6. PTHR10145:SF6. 2 hits.
PfamiPF14878. DLD. 1 hit.
PF14635. HHH_7. 1 hit.
PF14641. HTH_44. 1 hit.
PF00575. S1. 1 hit.
PF14633. SH2_2. 1 hit.
PF14632. SPT6_acidic. 1 hit.
PF14639. YqgF. 1 hit.
[Graphical view]
PIRSFiPIRSF036947. Spt6. 1 hit.
SMARTiSM00316. S1. 1 hit.
SM00252. SH2. 1 hit.
SM00732. YqgFc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 2 hits.
SSF50249. SSF50249. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50126. S1. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62383-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDFVESEAE ESEEEYNHEG EVVPRVTKKF VEEEDDDEEE EEENLDDQDE
60 70 80 90 100
RGNLKDFIND DDDEEEGEED EGSDSGDSED DVGHKKRKRP SFDDRLEDDD
110 120 130 140 150
FDLIEENLGV KVKRGQKYRR VKKMSDDDED DEEEYGKEEH EKEAIAGEIF
160 170 180 190 200
QDEEGEEGQE AVEAPMAPPD EEEEDDEESD IDDFIVDDDG QPLKKPKWRK
210 220 230 240 250
KLPGYTDAAL QEAQEIFGVD FDYDEFEKYN EYDEELEEDY EYEDDETEGE
260 270 280 290 300
IRVRPKKTTK KRVSRRSIFE MYEPSELESS HLTDQDNEIR ATDLPERFQL
310 320 330 340 350
RSIPVKAAED DELEEEADWI YRNAFATPTI SLQDSCDYLD RGQPTSSFSR
360 370 380 390 400
KGPSTVQKIK EALGFMRNQH FEVPFIAFYR KEYVEPELHI NDLWRVWQWD
410 420 430 440 450
EKWTQLRIRK ENLTRLFEKM QAYQYEQISA DPDKPLADGI RALDTTDMER
460 470 480 490 500
LKDVQSMDEL KDVYNHFLLY YGRDIPKMQN AAKASRKKLK RIKEDGDEEG
510 520 530 540 550
EGEEAEDEEQ RGPELKQASR RDMYTICQSA GLDGLAKKFG LTPEQFGENL
560 570 580 590 600
RDSYQRHETE QFPAEPLELA KDYVCSQFPT PEAVLEGARY MVALQIAREP
610 620 630 640 650
LVRQVLRQTF QERAKLNITP TKKGRKDVDE AHYAYSFKYL KNKPVKELRD
660 670 680 690 700
DQFLKIGLAE DEGLLTIDIS IDMKGVEGYG NDQTYFEEIK QFYYRDEFSH
710 720 730 740 750
QVQEWNRQRT MAIERALQQF LYVQMAKELK NKLLAEARES VVKACSRKLY
760 770 780 790 800
NWLRVAPYRP DQQVEEDDDF MDENQGKGIR VLGIAFSSAR DHPVFCALVN
810 820 830 840 850
GEGEVTDFLR LPHFTKRRTA WREEEREKKA QDIETLKKFL VNKKPHVVTI
860 870 880 890 900
AGENRDAQML TEDVKRIVHE LDQGQQLSSI GVELVDNELA ILYMNSKKSE
910 920 930 940 950
AEFRDYPPVL RQAVSLARRI QDPLIEFAQV CSSDEDILCL KFHPLQEHVV
960 970 980 990 1000
KEELLNALYC EFINRVNEVG VDVNRAIAHP YSQALIQYVC GLGPRKGTHL
1010 1020 1030 1040 1050
LKILKQNNTR LESRTQLVTM CHMGPKVFMN CAGFLKIDTA SLGDSTDSYI
1060 1070 1080 1090 1100
EVLDGSRVHP ETYEWARKMA VDALEYDESA EDANPAGALE EILENPERLK
1110 1120 1130 1140 1150
DLDLDAFAEE LERQGYGDKH ITLYDIRAEL SCRYKDLRTA YRSPNTEEIF
1160 1170 1180 1190 1200
NMLTKETPET FYIGKLIICN VTGIAHRRPQ GESYDQAIRN DETGLWQCPF
1210 1220 1230 1240 1250
CQQDNFPELS EVWNHFDSGS CPGQAIGVKT RLDNGVTGFI PTKFLSDKVV
1260 1270 1280 1290 1300
KRPEERVKVG MTVHCRIMKI DIEKFSADLT CRTSDLMDRN NEWKLPKDTY
1310 1320 1330 1340 1350
YDFDAEAADH KQEEDMKRKQ QRTTYIKRVI AHPSFHNINF KQAEKMMETM
1360 1370 1380 1390 1400
DQGDVIIRPS SKGENHLTVT WKVSAGIYQH VDVREEGKEN AFSLGATLWI
1410 1420 1430 1440 1450
NSEEFEDLDE IVARYVQPMA SFARDLLNHK YYQDCSGGDR KKLEELLIKT
1460 1470 1480 1490 1500
KKEKPTFIPY FICACKELPG KFLLGYQPRG KPRIEYVTVT PEGFRYRGQI
1510 1520 1530 1540 1550
FPTVNGLFRW FKDHYQDPVP GITPSSSNRT RTPASINATP ANINLADLTR
1560 1570 1580 1590 1600
AVNALPQNMT SQMFSAIAAV TGQGQNPNAT PAQWASSQYG YGGSGGGSSA
1610 1620 1630 1640 1650
YHVFPTPAQQ PVATPLMTPS YSYTTPSQPI TTPQYHQLQA STTPQSTQAQ
1660 1670 1680 1690 1700
PQPSSSSRQR QQQPKSNSHA AIDWGKMAEQ WLQEKEAERR KQKQRLTPRP
1710 1720
SPSPMIESTP MSIAGDATPL LDEMDR
Length:1,726
Mass (Da):199,086
Last modified:August 31, 2004 - v2
Checksum:i98BD891493372166
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti609 – 6091T → A in AAB18950 (PubMed:8786132).Curated
Sequence conflicti1520 – 15201P → Q in BAC32616 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40375 mRNA. Translation: AAB18950.1.
AL591070 Genomic DNA. Translation: CAI24323.1.
BC072657 mRNA. Translation: AAH72657.1.
AK129069 mRNA. Translation: BAC97879.1.
AK046156 mRNA. Translation: BAC32616.1.
CCDSiCCDS25095.1.
PIRiT30810.
RefSeqiNP_033323.2. NM_009297.2.
UniGeneiMm.20755.

Genome annotation databases

EnsembliENSMUST00000002121; ENSMUSP00000002121; ENSMUSG00000002052.
GeneIDi20926.
KEGGimmu:20926.
UCSCiuc007kip.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40375 mRNA. Translation: AAB18950.1.
AL591070 Genomic DNA. Translation: CAI24323.1.
BC072657 mRNA. Translation: AAH72657.1.
AK129069 mRNA. Translation: BAC97879.1.
AK046156 mRNA. Translation: BAC32616.1.
CCDSiCCDS25095.1.
PIRiT30810.
RefSeqiNP_033323.2. NM_009297.2.
UniGeneiMm.20755.

3D structure databases

ProteinModelPortaliQ62383.
SMRiQ62383. Positions 270-1176, 1326-1512.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203576. 5 interactions.
IntActiQ62383. 4 interactions.
MINTiMINT-4136149.
STRINGi10090.ENSMUSP00000002121.

PTM databases

iPTMnetiQ62383.
PhosphoSiteiQ62383.

Proteomic databases

EPDiQ62383.
MaxQBiQ62383.
PaxDbiQ62383.
PeptideAtlasiQ62383.
PRIDEiQ62383.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002121; ENSMUSP00000002121; ENSMUSG00000002052.
GeneIDi20926.
KEGGimmu:20926.
UCSCiuc007kip.2. mouse.

Organism-specific databases

CTDi20926.
MGIiMGI:107726. Supt6.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1856. Eukaryota.
COG2183. LUCA.
GeneTreeiENSGT00510000047446.
HOGENOMiHOG000001562.
HOVERGENiHBG093994.
InParanoidiQ62383.
KOiK11292.
OMAiDLKFRAF.
OrthoDBiEOG091G00SW.
PhylomeDBiQ62383.
TreeFamiTF105956.

Miscellaneous databases

PROiQ62383.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002052.
CleanExiMM_SUPT6H.
GenevisibleiQ62383. MM.

Family and domain databases

Gene3Di1.10.150.310. 1 hit.
1.10.3500.10. 2 hits.
2.40.50.140. 1 hit.
3.30.420.140. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR012337. RNaseH-like_dom.
IPR010994. RuvA_2-like.
IPR022967. S1_dom.
IPR003029. S1_domain.
IPR000980. SH2.
IPR028083. Spt6_acidic_N_dom.
IPR027999. Spt6_dom.
IPR032706. Spt6_HHH.
IPR028088. Spt6_HTH_DNA-bd_dom.
IPR028231. Spt6_YqgF.
IPR023323. Tex-like_dom.
IPR023097. Tex_RuvX-like_dom.
IPR017072. TF_Spt6.
IPR006641. YqgF/RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10145:SF6. PTHR10145:SF6. 2 hits.
PfamiPF14878. DLD. 1 hit.
PF14635. HHH_7. 1 hit.
PF14641. HTH_44. 1 hit.
PF00575. S1. 1 hit.
PF14633. SH2_2. 1 hit.
PF14632. SPT6_acidic. 1 hit.
PF14639. YqgF. 1 hit.
[Graphical view]
PIRSFiPIRSF036947. Spt6. 1 hit.
SMARTiSM00316. S1. 1 hit.
SM00252. SH2. 1 hit.
SM00732. YqgFc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 2 hits.
SSF50249. SSF50249. 1 hit.
SSF53098. SSF53098. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50126. S1. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPT6H_MOUSE
AccessioniPrimary (citable) accession number: Q62383
Secondary accession number(s): Q5SYM0
, Q6GQS3, Q6ZQI0, Q8BQY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: September 7, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.