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Q62381 (TLL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tolloid-like protein 1
Short name=mTll
EC=3.4.24.-
Gene names
Name:Tll1
Synonyms:Tll
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1013 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protease which processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Required for the embryonic development, especially heart development. Predominant protease, which in the development, influences dorsal-ventral patterning and skeletogenesis. Ref.4

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted Probable.

Tissue specificity

Highly expressed in brain and kidney and weakly in lung, skeletal muscle. A perceptible level of expression is observed in heart and testis. Ref.1

Developmental stage

Expressed at detectable level on E7, at increased level on E11, at maximal level on E15. Then, expression decreases from E17. Expressed in the developing neural tube. Ref.1

Sequence similarities

Belongs to the peptidase M12A family.

Contains 5 CUB domains.

Contains 2 EGF-like domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q62381-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q62381-2)

The sequence of this isoform differs from the canonical sequence as follows:
     387-390: IVLN → VVDT
     391-1013: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Propeptide31 – 147117 By similarity
PRO_0000046025
Chain148 – 1013866Tolloid-like protein 1
PRO_0000046026

Regions

Domain349 – 461113CUB 1
Domain462 – 574113CUB 2
Domain574 – 61542EGF-like 1; calcium-binding Potential
Domain618 – 730113CUB 3
Domain730 – 77041EGF-like 2; calcium-binding Potential
Domain774 – 886113CUB 4
Domain887 – 1003117CUB 5
Region148 – 348201Metalloprotease By similarity

Sites

Active site2411 By similarity
Metal binding2401Zinc; catalytic By similarity
Metal binding2441Zinc; catalytic By similarity
Metal binding2501Zinc; catalytic By similarity

Amino acid modifications

Glycosylation1691N-linked (GlcNAc...) Potential
Glycosylation3591N-linked (GlcNAc...) Potential
Glycosylation3901N-linked (GlcNAc...) Potential
Glycosylation6261N-linked (GlcNAc...) Potential
Disulfide bond349 ↔ 375 By similarity
Disulfide bond402 ↔ 424 By similarity
Disulfide bond462 ↔ 488 By similarity
Disulfide bond515 ↔ 537 By similarity
Disulfide bond578 ↔ 590 By similarity
Disulfide bond586 ↔ 599 By similarity
Disulfide bond601 ↔ 614 By similarity
Disulfide bond618 ↔ 644 By similarity
Disulfide bond671 ↔ 693 By similarity
Disulfide bond734 ↔ 745 By similarity
Disulfide bond741 ↔ 754 By similarity
Disulfide bond756 ↔ 769 By similarity
Disulfide bond774 ↔ 800 By similarity
Disulfide bond827 ↔ 849 By similarity
Disulfide bond887 ↔ 917 By similarity
Disulfide bond944 ↔ 966 By similarity

Natural variations

Alternative sequence387 – 3904IVLN → VVDT in isoform 2.
VSP_017199
Alternative sequence391 – 1013623Missing in isoform 2.
VSP_017200

Experimental info

Sequence conflict6821P → L in BAE23891. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B388914642BDD078

FASTA1,013114,533
        10         20         30         40         50         60 
MGLQALSPRM LLWLVVSGIV FSRVLWVCAG LDYDYTFDGN EEDKTEPIDY KDPCKAAVFW 

        70         80         90        100        110        120 
GDIALDDEDL NIFQIDRTID LTQSPFGKLG HITGGFGDHG MPKKRGALYQ LIERIRRIGS 

       130        140        150        160        170        180 
GLEQNNTMKG KAPPKLSEQS EKNRVPRAAT SRTERIWPGG VIPYVIGGNF TGSQRAMFKQ 

       190        200        210        220        230        240 
AMRHWEKHTC VTFTERSDEE SYIVFTYRPC GCCSYVGRRG NGPQAISIGK NCDKFGIVVH 

       250        260        270        280        290        300 
ELGHVIGFWH EHTRPDRDNH VTIIRENIQP GQEYNFLKME PGEVNSLGER YDFDSIMHYA 

       310        320        330        340        350        360 
RNTFSRGMFL DTILPSRDDN GIRPAIGQRT RLSKGDIAQA RKLYRCPACG ETLQESSGNL 

       370        380        390        400        410        420 
SSPGFPNGYP SYTHCIWRVS VTPGEKIVLN FTTMDLYKSS LCWYDYIEVR DGYWRKSPLL 

       430        440        450        460        470        480 
GRFCGDKVAG VLTSTDSRMW IEFRSSSNWV GKGFAAVYEA ICGGEIRKNE GQIQSPNYPD 

       490        500        510        520        530        540 
DYRPMKECVW KIMVSEGYHV GLTFQAFEIE RHDSCAYDHL EVRDGASENS PLIGRFCGYD 

       550        560        570        580        590        600 
KPEDIRSTSN TLWMKFVSDG TVNKAGFAAN FFKEEDECAK PDRGGCEQRC LNTLGSYQCA 

       610        620        630        640        650        660 
CEPGYELGPD RRSCEAACGG LLTKLNGTIT TPGWPKEYPP NKNCVWQVIA PSQYRISVKF 

       670        680        690        700        710        720 
EFFELEGNEV CKYDYVEIWS GPSSESKLHG KFCGADIPEV MTSHFNNMRI EFKSDNTVSK 

       730        740        750        760        770        780 
KGFKAHFFSD KDECSKDNGG CQHECVNTMG SYTCQCRNGF VLHENKHDCK EAECEQKIHS 

       790        800        810        820        830        840 
PSGLITSPNW PDKYPSRKEC TWVISAIPGH RITLAFNEFE VEQHQECAYD HLEIFDGETE 

       850        860        870        880        890        900 
KSPILGRLCG SKIPDPLMAT GNEMFIRFIS DASVQRKGFQ ATHSTECGGR LKAESKPRDL 

       910        920        930        940        950        960 
YSHAQFGDNN YPGQLDCEWL LVSERGSRLE LSFQTFEVEE EADCGYDYVE VFDGLSSKAV 

       970        980        990       1000       1010 
GLGRFCGSGP PEEIYSIGDV ALIHFHTDDT INKKGFYIRY KSIRYPETMH AKN

« Hide

Isoform 2 [UniParc].

Checksum: 31193DE9826893E8
Show »

FASTA39043,967

References

« Hide 'large scale' references
[1]"Characterization of a novel gene product (mammalian tolloid-like) with high sequence similarity to mammalian tolloid/bone morphogenetic protein-1."
Takahara K., Brevard R., Hoffman G.G., Suzuki N., Greenspan D.S.
Genomics 34:157-165(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 535-1013 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Adipose tissue and Cerebellum.
[3]"Mammalian BMP-1/Tolloid-related metalloproteinases, including novel family member mammalian Tolloid-like 2, have differential enzymatic activities and distributions of expression relevant to patterning and skeletogenesis."
Scott I.C., Blitz I.L., Pappano W.N., Imamura Y., Clark T.G., Steiglitz B.M., Thomas C.L., Maas S.A., Takahara K., Cho K.W., Greenspan D.S.
Dev. Biol. 213:283-300(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF ACTION ON CHORDIN.
[4]"The mammalian Tolloid-like 1 gene, Tll1, is necessary for normal septation and positioning of the heart."
Clark T.G., Conway S.J., Scott I.C., Labosky P.A., Winnier G., Bundy J., Hogan B.L., Greenspan D.S.
Development 126:2631-2642(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Bone morphogenetic protein-1 processes probiglycan."
Scott I.C., Imamura Y., Pappano W.N., Troedel J.M., Recklies A.D., Roughley P.J., Greenspan D.S.
J. Biol. Chem. 275:30504-30511(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF ACTION ON PRO-BIGLYCAN.
[6]"Multiple bone morphogenetic protein 1-related mammalian metalloproteinases process pro-lysyl oxidase at the correct physiological site and control lysyl oxidase activation in mouse embryo fibroblast cultures."
Uzel M.I., Scott I.C., Babakhanlou-Chase H., Palamakumbura A.H., Pappano W.N., Hong H.-H., Greenspan D.S., Trackman P.C.
J. Biol. Chem. 276:22537-22543(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION OF ACTION ON PRO-LYSYL OXIDASE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U34042 mRNA. Translation: AAC52654.1.
AK080919 mRNA. Translation: BAC38078.1.
AK139103 mRNA. Translation: BAE23891.1.
IPIIPI00124795.
IPI00720182.
RefSeqNP_033416.2. NM_009390.2.
UniGeneMm.418367.
Mm.438712.

3D structure databases

ProteinModelPortalQ62381.
SMRQ62381. Positions 148-1001.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000070560.

Protein family/group databases

MEROPSM12.016.

PTM databases

PhosphoSiteQ62381.

Proteomic databases

PRIDEQ62381.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID21892.
KEGGmmu:21892.
UCSCuc009lut.1. mouse.
uc009luu.1. mouse.

Organism-specific databases

CTD7092.
MGIMGI:106923. Tll1.

Phylogenomic databases

eggNOGNOG70307.
HOGENOMHOG000236339.
HOVERGENHBG004859.
InParanoidQ62381.
KOK09608.
OrthoDBEOG4FTVZV.

Gene expression databases

CleanExMM_TLL1.
GenevestigatorQ62381.
GermOnlineENSMUSG00000053626. Mus musculus.

Family and domain databases

Gene3D2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProIPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
PF07645. EGF_CA. 2 hits.
[Graphical view]
PIRSFPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSPR00480. ASTACIN.
SMARTSM00042. CUB. 5 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMSSF49854. CUB. 5 hits.
PROSITEPS01180. CUB. 5 hits.
PS00022. EGF_1. False negative.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSTLL1. mouse.
NextBio301420.
SOURCESearch...

Entry information

Entry nameTLL1_MOUSE
AccessionPrimary (citable) accession number: Q62381
Secondary accession number(s): Q3UTT9, Q8BNP5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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