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Protein

Tolloid-like protein 1

Gene

Tll1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease which processes procollagen C-propeptides, such as chordin, pro-biglycan and pro-lysyl oxidase. Required for the embryonic development, especially heart development. Predominant protease, which in the development, influences dorsal-ventral patterning and skeletogenesis.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi240 – 2401Zinc; catalyticPROSITE-ProRule annotation
Active sitei241 – 2411PROSITE-ProRule annotation
Metal bindingi244 – 2441Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi250 – 2501Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • collagen binding Source: MGI
  • metalloendopeptidase activity Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Differentiation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.016.

Names & Taxonomyi

Protein namesi
Recommended name:
Tolloid-like protein 1 (EC:3.4.24.-)
Short name:
mTll
Gene namesi
Name:Tll1
Synonyms:Tll
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:106923. Tll1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence analysisAdd
BLAST
Propeptidei31 – 147117By similarityPRO_0000046025Add
BLAST
Chaini148 – 1013866Tolloid-like protein 1PRO_0000046026Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence analysis
Disulfide bondi349 ↔ 375By similarity
Glycosylationi359 – 3591N-linked (GlcNAc...)Sequence analysis
Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence analysis
Disulfide bondi402 ↔ 424By similarity
Disulfide bondi462 ↔ 488By similarity
Disulfide bondi515 ↔ 537By similarity
Disulfide bondi578 ↔ 590By similarity
Disulfide bondi586 ↔ 599By similarity
Disulfide bondi601 ↔ 614By similarity
Disulfide bondi618 ↔ 644By similarity
Glycosylationi626 – 6261N-linked (GlcNAc...)Sequence analysis
Disulfide bondi671 ↔ 693By similarity
Disulfide bondi734 ↔ 745By similarity
Disulfide bondi741 ↔ 754By similarity
Disulfide bondi756 ↔ 769By similarity
Disulfide bondi774 ↔ 800By similarity
Disulfide bondi827 ↔ 849By similarity
Disulfide bondi887 ↔ 917By similarity
Disulfide bondi944 ↔ 966By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ62381.
PaxDbiQ62381.
PeptideAtlasiQ62381.
PRIDEiQ62381.

PTM databases

iPTMnetiQ62381.
PhosphoSiteiQ62381.

Expressioni

Tissue specificityi

Highly expressed in brain and kidney and weakly in lung, skeletal muscle. A perceptible level of expression is observed in heart and testis.1 Publication

Developmental stagei

Expressed at detectable level on E7, at increased level on E11, at maximal level on E15. Then, expression decreases from E17. Expressed in the developing neural tube.1 Publication

Gene expression databases

CleanExiMM_TLL1.

Interactioni

GO - Molecular functioni

  • collagen binding Source: MGI

Protein-protein interaction databases

BioGridi204220. 2 interactions.
IntActiQ62381. 3 interactions.
STRINGi10090.ENSMUSP00000070560.

Structurei

3D structure databases

ProteinModelPortaliQ62381.
SMRiQ62381. Positions 148-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini349 – 461113CUB 1PROSITE-ProRule annotationAdd
BLAST
Domaini462 – 574113CUB 2PROSITE-ProRule annotationAdd
BLAST
Domaini574 – 61542EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini618 – 730113CUB 3PROSITE-ProRule annotationAdd
BLAST
Domaini730 – 77041EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini774 – 886113CUB 4PROSITE-ProRule annotationAdd
BLAST
Domaini887 – 1003117CUB 5PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 348201MetalloproteaseBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M12A family.Curated
Contains 5 CUB domains.PROSITE-ProRule annotation
Contains 2 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
HOGENOMiHOG000236339.
HOVERGENiHBG004859.
InParanoidiQ62381.
KOiK09608.
PhylomeDBiQ62381.

Family and domain databases

Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62381-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLQALSPRM LLWLVVSGIV FSRVLWVCAG LDYDYTFDGN EEDKTEPIDY
60 70 80 90 100
KDPCKAAVFW GDIALDDEDL NIFQIDRTID LTQSPFGKLG HITGGFGDHG
110 120 130 140 150
MPKKRGALYQ LIERIRRIGS GLEQNNTMKG KAPPKLSEQS EKNRVPRAAT
160 170 180 190 200
SRTERIWPGG VIPYVIGGNF TGSQRAMFKQ AMRHWEKHTC VTFTERSDEE
210 220 230 240 250
SYIVFTYRPC GCCSYVGRRG NGPQAISIGK NCDKFGIVVH ELGHVIGFWH
260 270 280 290 300
EHTRPDRDNH VTIIRENIQP GQEYNFLKME PGEVNSLGER YDFDSIMHYA
310 320 330 340 350
RNTFSRGMFL DTILPSRDDN GIRPAIGQRT RLSKGDIAQA RKLYRCPACG
360 370 380 390 400
ETLQESSGNL SSPGFPNGYP SYTHCIWRVS VTPGEKIVLN FTTMDLYKSS
410 420 430 440 450
LCWYDYIEVR DGYWRKSPLL GRFCGDKVAG VLTSTDSRMW IEFRSSSNWV
460 470 480 490 500
GKGFAAVYEA ICGGEIRKNE GQIQSPNYPD DYRPMKECVW KIMVSEGYHV
510 520 530 540 550
GLTFQAFEIE RHDSCAYDHL EVRDGASENS PLIGRFCGYD KPEDIRSTSN
560 570 580 590 600
TLWMKFVSDG TVNKAGFAAN FFKEEDECAK PDRGGCEQRC LNTLGSYQCA
610 620 630 640 650
CEPGYELGPD RRSCEAACGG LLTKLNGTIT TPGWPKEYPP NKNCVWQVIA
660 670 680 690 700
PSQYRISVKF EFFELEGNEV CKYDYVEIWS GPSSESKLHG KFCGADIPEV
710 720 730 740 750
MTSHFNNMRI EFKSDNTVSK KGFKAHFFSD KDECSKDNGG CQHECVNTMG
760 770 780 790 800
SYTCQCRNGF VLHENKHDCK EAECEQKIHS PSGLITSPNW PDKYPSRKEC
810 820 830 840 850
TWVISAIPGH RITLAFNEFE VEQHQECAYD HLEIFDGETE KSPILGRLCG
860 870 880 890 900
SKIPDPLMAT GNEMFIRFIS DASVQRKGFQ ATHSTECGGR LKAESKPRDL
910 920 930 940 950
YSHAQFGDNN YPGQLDCEWL LVSERGSRLE LSFQTFEVEE EADCGYDYVE
960 970 980 990 1000
VFDGLSSKAV GLGRFCGSGP PEEIYSIGDV ALIHFHTDDT INKKGFYIRY
1010
KSIRYPETMH AKN
Length:1,013
Mass (Da):114,533
Last modified:November 1, 1996 - v1
Checksum:iB388914642BDD078
GO
Isoform 2 (identifier: Q62381-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     387-390: IVLN → VVDT
     391-1013: Missing.

Note: No experimental confirmation available.
Show »
Length:390
Mass (Da):43,967
Checksum:i31193DE9826893E8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti682 – 6821P → L in BAE23891 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei387 – 3904IVLN → VVDT in isoform 2. 1 PublicationVSP_017199
Alternative sequencei391 – 1013623Missing in isoform 2. 1 PublicationVSP_017200Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34042 mRNA. Translation: AAC52654.1.
AK080919 mRNA. Translation: BAC38078.1.
AK139103 mRNA. Translation: BAE23891.1.
CCDSiCCDS40352.1. [Q62381-1]
RefSeqiNP_033416.2. NM_009390.2.
UniGeneiMm.418367.
Mm.438712.

Genome annotation databases

GeneIDi21892.
KEGGimmu:21892.
UCSCiuc009luu.1. mouse. [Q62381-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34042 mRNA. Translation: AAC52654.1.
AK080919 mRNA. Translation: BAC38078.1.
AK139103 mRNA. Translation: BAE23891.1.
CCDSiCCDS40352.1. [Q62381-1]
RefSeqiNP_033416.2. NM_009390.2.
UniGeneiMm.418367.
Mm.438712.

3D structure databases

ProteinModelPortaliQ62381.
SMRiQ62381. Positions 148-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204220. 2 interactions.
IntActiQ62381. 3 interactions.
STRINGi10090.ENSMUSP00000070560.

Protein family/group databases

MEROPSiM12.016.

PTM databases

iPTMnetiQ62381.
PhosphoSiteiQ62381.

Proteomic databases

MaxQBiQ62381.
PaxDbiQ62381.
PeptideAtlasiQ62381.
PRIDEiQ62381.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi21892.
KEGGimmu:21892.
UCSCiuc009luu.1. mouse. [Q62381-2]

Organism-specific databases

CTDi7092.
MGIiMGI:106923. Tll1.

Phylogenomic databases

eggNOGiKOG3714. Eukaryota.
ENOG410ZPX7. LUCA.
HOGENOMiHOG000236339.
HOVERGENiHBG004859.
InParanoidiQ62381.
KOiK09608.
PhylomeDBiQ62381.

Miscellaneous databases

ChiTaRSiTll1. mouse.
PROiQ62381.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TLL1.

Family and domain databases

Gene3Di2.60.120.290. 5 hits.
3.40.390.10. 1 hit.
InterProiIPR015446. BMP_1/tolloid-like.
IPR000859. CUB_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR024079. MetalloPept_cat_dom.
IPR001506. Peptidase_M12A.
IPR006026. Peptidase_Metallo.
[Graphical view]
PfamiPF01400. Astacin. 1 hit.
PF00431. CUB. 5 hits.
[Graphical view]
PIRSFiPIRSF001199. BMP_1/tolloid-like. 1 hit.
PRINTSiPR00480. ASTACIN.
SMARTiSM00042. CUB. 5 hits.
SM00181. EGF. 2 hits.
SM00179. EGF_CA. 2 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 5 hits.
PROSITEiPS01180. CUB. 5 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 2 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTLL1_MOUSE
AccessioniPrimary (citable) accession number: Q62381
Secondary accession number(s): Q3UTT9, Q8BNP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.