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Protein

U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2

Gene

Zrsr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri170 – 198C3H1-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri310 – 337C3H1-type 2PROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2
Alternative name(s):
CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 2
U2(RNU2) small nuclear RNA auxiliary factor 1-like 2
U2AF35-related protein
Short name:
URP
Gene namesi
Name:Zrsr2
Synonyms:U2af1-rs2, U2af1l2, U2af1rs2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:103287. Zrsr2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000820021 – 462U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2Add BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei353PhosphoserineCombined sources1
Modified residuei389PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated in the RS domain by SRPK1.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ62377.
MaxQBiQ62377.
PaxDbiQ62377.
PeptideAtlasiQ62377.
PRIDEiQ62377.

PTM databases

iPTMnetiQ62377.

Interactioni

Subunit structurei

Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Interacts (via RS domain) with SRSF1 and SRSF2. Interacts with U2AF2/U2AF65 (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-4084219.
STRINGi10090.ENSMUSP00000107908.

Structurei

3D structure databases

ProteinModelPortaliQ62377.
SMRiQ62377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini202 – 308RRMPROSITE-ProRule annotationAdd BLAST107

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi50 – 53Poly-Glu4
Compositional biasi93 – 96Poly-Lys4
Compositional biasi122 – 127Poly-Glu6
Compositional biasi380 – 386Poly-Arg7

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri170 – 198C3H1-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri310 – 337C3H1-type 2PROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2202. Eukaryota.
ENOG410Z5PX. LUCA.
HOGENOMiHOG000049279.
HOVERGENiHBG054605.
InParanoidiQ62377.

Family and domain databases

Gene3Di4.10.1000.10. 2 hits.
InterProiView protein in InterPro
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR009145. U2AF_small.
IPR000571. Znf_CCCH.
PANTHERiPTHR12620. PTHR12620. 1 hit.
PfamiView protein in Pfam
PF00076. RRM_1. 1 hit.
PF00642. zf-CCCH. 1 hit.
PRINTSiPR01848. U2AUXFACTOR.
SMARTiView protein in SMART
SM00361. RRM_1. 1 hit.
SM00356. ZnF_C3H1. 2 hits.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 2 hits.

Sequencei

Sequence statusi: Complete.

Q62377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METAGATADA TAGPQKLSRK KYLALRKKER RKRRRQALAR LREAELAQKE
60 70 80 90 100
EEEDPLAEEK RLEEERLLEE ERQRLHEEWL LREEKAQEEF RAKKKKEEEA
110 120 130 140 150
RKRKEELERK LKAEWEEQQR KEREEEEQKR QEKREREEAV QKMLDQAENE
160 170 180 190 200
LENGGTWQNP EPPMDIRVLE KDRANCPFYS KTGACRFGDR CSRKHNFPTS
210 220 230 240 250
SPTLLIKGMF TTFGMEQCRR DDYDPDSSLE FSEEEIYQQF LDFYYDVLPE
260 270 280 290 300
FKSVGKVIQF KVSCNLEPHL RGNVYVQYQS EEDCQAAFSV FNGRWYAGRQ
310 320 330 340 350
LQCEFCPVTR WKMAICGLFE VQQCPRGKHC NFLHVFRNPN NEYRDANRDL
360 370 380 390 400
YPSPDWTSSS FGKNSERRER ASHYDEYYGR SRRRRRSPSP DFYKRNGESD
410 420 430 440 450
RKSSSRHRVK KSHRYGMKSR ERRSSPSRRR KDHSPGPWEP EQEEPPQQES
460
QSQPQPQPQS DP
Length:462
Mass (Da):55,358
Last modified:November 1, 1996 - v1
Checksum:i0E980C029ACBA196
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45205 mRNA. Translation: BAA08143.1.
PIRiA57120.
UniGeneiMm.426178.

Similar proteinsi

Entry informationi

Entry nameiU2AFM_MOUSE
AccessioniPrimary (citable) accession number: Q62377
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: August 30, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot