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Protein

U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2

Gene

Zrsr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pre-mRNA-binding protein required for splicing of both U2- and U12-type introns. Selectively interacts with the 3'-splice site of U2- and U12-type pre-mRNAs and promotes different steps in U2 and U12 intron splicing. Recruited to U12 pre-mRNAs in an ATP-dependent manner and is required for assembly of the prespliceosome, a precursor to other spliceosomal complexes. For U2-type introns, it is selectively and specifically required for the second step of splicing (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri170 – 19829C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri310 – 33728C3H1-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2
Alternative name(s):
CCCH type zinc finger, RNA-binding motif and serine/arginine rich protein 2
U2(RNU2) small nuclear RNA auxiliary factor 1-like 2
U2AF35-related protein
Short name:
URP
Gene namesi
Name:Zrsr2
Synonyms:U2af1-rs2, U2af1l2, U2af1rs2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:103287. Zrsr2.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: MGI
  • U12-type spliceosomal complex Source: UniProtKB
  • U2AF Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit-related protein 2PRO_0000082002Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei353 – 3531PhosphoserineCombined sources
Modified residuei389 – 3891PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated in the RS domain by SRPK1.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ62377.
MaxQBiQ62377.
PaxDbiQ62377.
PeptideAtlasiQ62377.
PRIDEiQ62377.

PTM databases

iPTMnetiQ62377.
PhosphoSiteiQ62377.

Interactioni

Subunit structurei

Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Interacts (via RS domain) with SRSF1 and SRSF2. Interacts with U2AF2/U2AF65 (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-4084219.
STRINGi10090.ENSMUSP00000107908.

Structurei

3D structure databases

ProteinModelPortaliQ62377.
SMRiQ62377. Positions 173-345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 308107RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi50 – 534Poly-Glu
Compositional biasi93 – 964Poly-Lys
Compositional biasi122 – 1276Poly-Glu
Compositional biasi380 – 3867Poly-Arg

Sequence similaritiesi

Contains 2 C3H1-type zinc fingers.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri170 – 19829C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri310 – 33728C3H1-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2202. Eukaryota.
ENOG410Z5PX. LUCA.
HOGENOMiHOG000049279.
HOVERGENiHBG054605.
InParanoidiQ62377.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR009145. U2AF_small.
IPR000571. Znf_CCCH.
[Graphical view]
PANTHERiPTHR12620. PTHR12620. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
PF00642. zf-CCCH. 1 hit.
[Graphical view]
PRINTSiPR01848. U2AUXFACTOR.
SMARTiSM00361. RRM_1. 1 hit.
SM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METAGATADA TAGPQKLSRK KYLALRKKER RKRRRQALAR LREAELAQKE
60 70 80 90 100
EEEDPLAEEK RLEEERLLEE ERQRLHEEWL LREEKAQEEF RAKKKKEEEA
110 120 130 140 150
RKRKEELERK LKAEWEEQQR KEREEEEQKR QEKREREEAV QKMLDQAENE
160 170 180 190 200
LENGGTWQNP EPPMDIRVLE KDRANCPFYS KTGACRFGDR CSRKHNFPTS
210 220 230 240 250
SPTLLIKGMF TTFGMEQCRR DDYDPDSSLE FSEEEIYQQF LDFYYDVLPE
260 270 280 290 300
FKSVGKVIQF KVSCNLEPHL RGNVYVQYQS EEDCQAAFSV FNGRWYAGRQ
310 320 330 340 350
LQCEFCPVTR WKMAICGLFE VQQCPRGKHC NFLHVFRNPN NEYRDANRDL
360 370 380 390 400
YPSPDWTSSS FGKNSERRER ASHYDEYYGR SRRRRRSPSP DFYKRNGESD
410 420 430 440 450
RKSSSRHRVK KSHRYGMKSR ERRSSPSRRR KDHSPGPWEP EQEEPPQQES
460
QSQPQPQPQS DP
Length:462
Mass (Da):55,358
Last modified:November 1, 1996 - v1
Checksum:i0E980C029ACBA196
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45205 mRNA. Translation: BAA08143.1.
PIRiA57120.
UniGeneiMm.426178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45205 mRNA. Translation: BAA08143.1.
PIRiA57120.
UniGeneiMm.426178.

3D structure databases

ProteinModelPortaliQ62377.
SMRiQ62377. Positions 173-345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4084219.
STRINGi10090.ENSMUSP00000107908.

PTM databases

iPTMnetiQ62377.
PhosphoSiteiQ62377.

Proteomic databases

EPDiQ62377.
MaxQBiQ62377.
PaxDbiQ62377.
PeptideAtlasiQ62377.
PRIDEiQ62377.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:103287. Zrsr2.

Phylogenomic databases

eggNOGiKOG2202. Eukaryota.
ENOG410Z5PX. LUCA.
HOGENOMiHOG000049279.
HOVERGENiHBG054605.
InParanoidiQ62377.

Miscellaneous databases

ChiTaRSiZrsr2. mouse.
PROiQ62377.
SOURCEiSearch...

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR009145. U2AF_small.
IPR000571. Znf_CCCH.
[Graphical view]
PANTHERiPTHR12620. PTHR12620. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
PF00642. zf-CCCH. 1 hit.
[Graphical view]
PRINTSiPR01848. U2AUXFACTOR.
SMARTiSM00361. RRM_1. 1 hit.
SM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and mapping of the U2af1-rs2 gene with a high transmission distortion in interspecific backcross progeny."
    Yamaoka T., Hatada I., Kitagawa K., Wang X., Mukai T.
    Genomics 27:337-340(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney.

Entry informationi

Entry nameiU2AFM_MOUSE
AccessioniPrimary (citable) accession number: Q62377
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.