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Q62376

- RU17_MOUSE

UniProt

Q62376 - RU17_MOUSE

Protein

U1 small nuclear ribonucleoprotein 70 kDa

Gene

Snrnp70

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA By similarity.By similarity

    GO - Molecular functioni

    1. nucleotide binding Source: InterPro
    2. RNA binding Source: UniProtKB

    GO - Biological processi

    1. mRNA splicing, via spliceosome Source: UniProtKB
    2. regulation of RNA splicing Source: UniProtKB

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    U1 small nuclear ribonucleoprotein 70 kDa
    Short name:
    U1 snRNP 70 kDa
    Short name:
    U1-70K
    Short name:
    snRNP70
    Gene namesi
    Name:Snrnp70
    Synonyms:Snrp70
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:98341. Snrnp70.

    Subcellular locationi

    Nucleus speckle 1 Publication. Nucleusnucleoplasm 1 Publication
    Note: Colocalizes with SCNM1 and LUC7L2 in nuclear speckles.

    GO - Cellular componenti

    1. nuclear speck Source: UniProtKB-SubCell
    2. nucleus Source: MGI
    3. spliceosomal complex Source: UniProtKB
    4. U1 snRNP Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 448447U1 small nuclear ribonucleoprotein 70 kDaPRO_0000081881Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonineBy similarity
    Modified residuei118 – 1181N6-acetyllysineBy similarity
    Modified residuei226 – 2261PhosphoserineBy similarity
    Modified residuei268 – 2681PhosphoserineBy similarity
    Modified residuei332 – 3321PhosphoserineBy similarity
    Modified residuei408 – 4081Phosphoserine2 Publications
    Modified residuei419 – 4191Phosphoserine1 Publication

    Post-translational modificationi

    Extensively phosphorylated on serine residues in the C-terminal region.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ62376.
    PaxDbiQ62376.
    PRIDEiQ62376.

    PTM databases

    PhosphoSiteiQ62376.

    Expressioni

    Gene expression databases

    ArrayExpressiQ62376.
    BgeeiQ62376.
    GenevestigatoriQ62376.

    Interactioni

    Subunit structurei

    U1 snRNP is composed of the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. Interacts with SCNM1. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated SFRS13A and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SCAF11, and ZRANB2.1 Publication

    Protein-protein interaction databases

    BioGridi203376. 2 interactions.
    IntActiQ62376. 2 interactions.
    MINTiMINT-1868298.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62376.
    SMRiQ62376. Positions 114-177.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini103 – 18179RRMPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi231 – 31080Arg/Glu-rich (mixed charge)Add
    BLAST
    Compositional biasi311 – 32616Poly-GlyAdd
    BLAST
    Compositional biasi356 – 40348Arg/Asp/Glu-rich (mixed charge)Add
    BLAST
    Compositional biasi404 – 4096Poly-Gly

    Sequence similaritiesi

    Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0724.
    GeneTreeiENSGT00530000063750.
    HOGENOMiHOG000236289.
    HOVERGENiHBG094947.
    InParanoidiQ62376.
    KOiK11093.
    OMAiDGRDMYM.
    OrthoDBiEOG79W96V.
    PhylomeDBiQ62376.
    TreeFamiTF314215.

    Family and domain databases

    Gene3Di3.30.70.330. 1 hit.
    InterProiIPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR022023. U1snRNP70_N.
    [Graphical view]
    PfamiPF00076. RRM_1. 1 hit.
    PF12220. U1snRNP70_N. 1 hit.
    [Graphical view]
    SMARTiSM00360. RRM. 1 hit.
    [Graphical view]
    PROSITEiPS50102. RRM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q62376-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTQFLPPNLL ALFAPRDPIP YLPPLEKLPH EKHHNQPYCG IAPYIREFED    50
    PRDAPPPTRA ETREERMERK RREKIERRQQ EVETELKMWD PHNDPNAQGD 100
    AFKTLFVARV NYDTTESKLR REFEVYGPIK RIHMVYSKRS GKPRGYAFIE 150
    YEHERDMHSA YKHADGKKID GRRVLVDVER GRTVKGWRPR RLGGGLGGTR 200
    RGGADVNIRH SGRDDTSRYD ERPGPSPLPH RDRDRDRERE RRERSRERDK 250
    ERERRRSRSR DRRRRSRSRD KDERRRSRER SKDKDRDRKR RSSRSRERAR 300
    RERERKEELR GGGGGGGGGS GGGGGGDMAE PSEAGDGAPD DGPPGELGPE 350
    GPDGPEEKGR DRDRERRRSH RSERERRRDR DRDRDREHKR GERGSERGRD 400
    EARGGGGSGQ DNGLEGLGSD GRDMYMEAEG GDGYMAPENG YLMEAAPE 448
    Length:448
    Mass (Da):51,992
    Last modified:December 6, 2005 - v2
    Checksum:i5B025A3B6992D0BD
    GO
    Isoform 2 (identifier: Q62376-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         160-166: AYKHADG → TTQLACS
         167-448: Missing.

    Show »
    Length:166
    Mass (Da):19,790
    Checksum:iE90F034A08D3E79C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti263 – 2631R → Q in CAA33777. (PubMed:2525092)Curated
    Sequence conflicti281 – 2811S → T in CAA33777. (PubMed:2525092)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei160 – 1667AYKHADG → TTQLACS in isoform 2. CuratedVSP_005851
    Alternative sequencei167 – 448282Missing in isoform 2. CuratedVSP_005852Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK133115 mRNA. Translation: BAE21515.1.
    AK146729 mRNA. Translation: BAE27392.1.
    X15769
    , X15770, X15771, X15772, X15774, X15775, X15776 Genomic DNA. Translation: CAA33777.1.
    CCDSiCCDS21240.1. [Q62376-1]
    PIRiS04336.
    S04824.
    RefSeqiNP_033250.3. NM_009224.4. [Q62376-1]
    XP_006540795.1. XM_006540732.1. [Q62376-1]
    UniGeneiMm.216386.

    Genome annotation databases

    EnsembliENSMUST00000074575; ENSMUSP00000074160; ENSMUSG00000063511. [Q62376-1]
    GeneIDi20637.
    KEGGimmu:20637.
    UCSCiuc009guw.1. mouse. [Q62376-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK133115 mRNA. Translation: BAE21515.1 .
    AK146729 mRNA. Translation: BAE27392.1 .
    X15769
    , X15770 , X15771 , X15772 , X15774 , X15775 , X15776 Genomic DNA. Translation: CAA33777.1 .
    CCDSi CCDS21240.1. [Q62376-1 ]
    PIRi S04336.
    S04824.
    RefSeqi NP_033250.3. NM_009224.4. [Q62376-1 ]
    XP_006540795.1. XM_006540732.1. [Q62376-1 ]
    UniGenei Mm.216386.

    3D structure databases

    ProteinModelPortali Q62376.
    SMRi Q62376. Positions 114-177.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203376. 2 interactions.
    IntActi Q62376. 2 interactions.
    MINTi MINT-1868298.

    PTM databases

    PhosphoSitei Q62376.

    Proteomic databases

    MaxQBi Q62376.
    PaxDbi Q62376.
    PRIDEi Q62376.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000074575 ; ENSMUSP00000074160 ; ENSMUSG00000063511 . [Q62376-1 ]
    GeneIDi 20637.
    KEGGi mmu:20637.
    UCSCi uc009guw.1. mouse. [Q62376-1 ]

    Organism-specific databases

    CTDi 6625.
    MGIi MGI:98341. Snrnp70.

    Phylogenomic databases

    eggNOGi COG0724.
    GeneTreei ENSGT00530000063750.
    HOGENOMi HOG000236289.
    HOVERGENi HBG094947.
    InParanoidi Q62376.
    KOi K11093.
    OMAi DGRDMYM.
    OrthoDBi EOG79W96V.
    PhylomeDBi Q62376.
    TreeFami TF314215.

    Miscellaneous databases

    ChiTaRSi SNRNP70. mouse.
    NextBioi 299035.
    PROi Q62376.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q62376.
    Bgeei Q62376.
    Genevestigatori Q62376.

    Family and domain databases

    Gene3Di 3.30.70.330. 1 hit.
    InterProi IPR012677. Nucleotide-bd_a/b_plait.
    IPR000504. RRM_dom.
    IPR022023. U1snRNP70_N.
    [Graphical view ]
    Pfami PF00076. RRM_1. 1 hit.
    PF12220. U1snRNP70_N. 1 hit.
    [Graphical view ]
    SMARTi SM00360. RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS50102. RRM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Kidney and Testis.
    2. "Analysis of genomic clones of the murine U1RNA-associated 70-kDa protein reveals a high evolutionary conservation of the protein between human and mouse."
      Hornig H., Fischer U., Costas M., Rauh A., Luehrmann R.
      Eur. J. Biochem. 182:45-50(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-448 (ISOFORMS 1 AND 2).
      Strain: BALB/c.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    4. "Evidence for a direct role of the disease modifier SCNM1 in splicing."
      Howell V.M., Jones J.M., Bergren S.K., Li L., Billi A.C., Avenarius M.R., Meisler M.H.
      Hum. Mol. Genet. 16:2506-2516(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SCNM1, SUBCELLULAR LOCATION.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408 AND SER-419, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiRU17_MOUSE
    AccessioniPrimary (citable) accession number: Q62376
    Secondary accession number(s): Q3UIW4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3