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Q62376 (RU17_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
U1 small nuclear ribonucleoprotein 70 kDa

Short name=U1 snRNP 70 kDa
Short name=U1-70K
Short name=snRNP70
Gene names
Name:Snrnp70
Synonyms:Snrp70
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA By similarity.

Subunit structure

U1 snRNP is composed of the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. Interacts with SCNM1. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated SFRS13A and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SCAF11, and ZRANB2. Ref.4

Subcellular location

Nucleus speckle. Nucleusnucleoplasm. Note: Colocalizes with SCNM1 and LUC7L2 in nuclear speckles. Ref.4

Post-translational modification

Extensively phosphorylated on serine residues in the C-terminal region By similarity.

Sequence similarities

Contains 1 RRM (RNA recognition motif) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q62376-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q62376-2)

The sequence of this isoform differs from the canonical sequence as follows:
     160-166: AYKHADG → TTQLACS
     167-448: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 448447U1 small nuclear ribonucleoprotein 70 kDa
PRO_0000081881

Regions

Domain103 – 18179RRM
Compositional bias231 – 31080Arg/Glu-rich (mixed charge)
Compositional bias311 – 32616Poly-Gly
Compositional bias356 – 40348Arg/Asp/Glu-rich (mixed charge)
Compositional bias404 – 4096Poly-Gly

Amino acid modifications

Modified residue21N-acetylthreonine By similarity
Modified residue1181N6-acetyllysine By similarity
Modified residue2261Phosphoserine By similarity
Modified residue2681Phosphoserine By similarity
Modified residue3321Phosphoserine By similarity
Modified residue4081Phosphoserine Ref.3 Ref.5
Modified residue4191Phosphoserine Ref.5

Natural variations

Alternative sequence160 – 1667AYKHADG → TTQLACS in isoform 2.
VSP_005851
Alternative sequence167 – 448282Missing in isoform 2.
VSP_005852

Experimental info

Sequence conflict2631R → Q in CAA33777. Ref.2
Sequence conflict2811S → T in CAA33777. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 6, 2005. Version 2.
Checksum: 5B025A3B6992D0BD

FASTA44851,992
        10         20         30         40         50         60 
MTQFLPPNLL ALFAPRDPIP YLPPLEKLPH EKHHNQPYCG IAPYIREFED PRDAPPPTRA 

        70         80         90        100        110        120 
ETREERMERK RREKIERRQQ EVETELKMWD PHNDPNAQGD AFKTLFVARV NYDTTESKLR 

       130        140        150        160        170        180 
REFEVYGPIK RIHMVYSKRS GKPRGYAFIE YEHERDMHSA YKHADGKKID GRRVLVDVER 

       190        200        210        220        230        240 
GRTVKGWRPR RLGGGLGGTR RGGADVNIRH SGRDDTSRYD ERPGPSPLPH RDRDRDRERE 

       250        260        270        280        290        300 
RRERSRERDK ERERRRSRSR DRRRRSRSRD KDERRRSRER SKDKDRDRKR RSSRSRERAR 

       310        320        330        340        350        360 
RERERKEELR GGGGGGGGGS GGGGGGDMAE PSEAGDGAPD DGPPGELGPE GPDGPEEKGR 

       370        380        390        400        410        420 
DRDRERRRSH RSERERRRDR DRDRDREHKR GERGSERGRD EARGGGGSGQ DNGLEGLGSD 

       430        440 
GRDMYMEAEG GDGYMAPENG YLMEAAPE 

« Hide

Isoform 2 [UniParc].

Checksum: E90F034A08D3E79C
Show »

FASTA16619,790

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Kidney and Testis.
[2]"Analysis of genomic clones of the murine U1RNA-associated 70-kDa protein reveals a high evolutionary conservation of the protein between human and mouse."
Hornig H., Fischer U., Costas M., Rauh A., Luehrmann R.
Eur. J. Biochem. 182:45-50(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-448 (ISOFORMS 1 AND 2).
Strain: BALB/c.
[3]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[4]"Evidence for a direct role of the disease modifier SCNM1 in splicing."
Howell V.M., Jones J.M., Bergren S.K., Li L., Billi A.C., Avenarius M.R., Meisler M.H.
Hum. Mol. Genet. 16:2506-2516(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SCNM1, SUBCELLULAR LOCATION.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408 AND SER-419, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK133115 mRNA. Translation: BAE21515.1.
AK146729 mRNA. Translation: BAE27392.1.
X15769 expand/collapse EMBL AC list , X15770, X15771, X15772, X15774, X15775, X15776 Genomic DNA. Translation: CAA33777.1.
CCDSCCDS21240.1. [Q62376-1]
PIRS04336.
S04824.
RefSeqNP_033250.3. NM_009224.4. [Q62376-1]
XP_006540795.1. XM_006540732.1. [Q62376-1]
UniGeneMm.216386.

3D structure databases

ProteinModelPortalQ62376.
SMRQ62376. Positions 114-177.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203376. 2 interactions.
IntActQ62376. 2 interactions.
MINTMINT-1868298.

PTM databases

PhosphoSiteQ62376.

Proteomic databases

MaxQBQ62376.
PaxDbQ62376.
PRIDEQ62376.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000074575; ENSMUSP00000074160; ENSMUSG00000063511. [Q62376-1]
GeneID20637.
KEGGmmu:20637.
UCSCuc009guw.1. mouse. [Q62376-1]

Organism-specific databases

CTD6625.
MGIMGI:98341. Snrnp70.

Phylogenomic databases

eggNOGCOG0724.
GeneTreeENSGT00530000063750.
HOGENOMHOG000236289.
HOVERGENHBG094947.
InParanoidQ62376.
KOK11093.
OMADGRDMYM.
OrthoDBEOG79W96V.
PhylomeDBQ62376.
TreeFamTF314215.

Gene expression databases

ArrayExpressQ62376.
BgeeQ62376.
GenevestigatorQ62376.

Family and domain databases

Gene3D3.30.70.330. 1 hit.
InterProIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR022023. U1snRNP70_N.
[Graphical view]
PfamPF00076. RRM_1. 1 hit.
PF12220. U1snRNP70_N. 1 hit.
[Graphical view]
SMARTSM00360. RRM. 1 hit.
[Graphical view]
PROSITEPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSNRNP70. mouse.
NextBio299035.
PROQ62376.
SOURCESearch...

Entry information

Entry nameRU17_MOUSE
AccessionPrimary (citable) accession number: Q62376
Secondary accession number(s): Q3UIW4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: December 6, 2005
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot