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Q62376

- RU17_MOUSE

UniProt

Q62376 - RU17_MOUSE

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Protein

U1 small nuclear ribonucleoprotein 70 kDa

Gene
Snrnp70, Snrp70
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA By similarity.

GO - Molecular functioni

  1. nucleotide binding Source: InterPro
  2. RNA binding Source: UniProtKB

GO - Biological processi

  1. mRNA splicing, via spliceosome Source: UniProtKB
  2. regulation of RNA splicing Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
U1 small nuclear ribonucleoprotein 70 kDa
Short name:
U1 snRNP 70 kDa
Short name:
U1-70K
Short name:
snRNP70
Gene namesi
Name:Snrnp70
Synonyms:Snrp70
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:98341. Snrnp70.

Subcellular locationi

Nucleus speckle. Nucleusnucleoplasm
Note: Colocalizes with SCNM1 and LUC7L2 in nuclear speckles.1 Publication

GO - Cellular componenti

  1. nuclear speck Source: UniProtKB-SubCell
  2. nucleus Source: MGI
  3. spliceosomal complex Source: UniProtKB
  4. U1 snRNP Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 448447U1 small nuclear ribonucleoprotein 70 kDaPRO_0000081881Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine By similarity
Modified residuei118 – 1181N6-acetyllysine By similarity
Modified residuei226 – 2261Phosphoserine By similarity
Modified residuei268 – 2681Phosphoserine By similarity
Modified residuei332 – 3321Phosphoserine By similarity
Modified residuei408 – 4081Phosphoserine2 Publications
Modified residuei419 – 4191Phosphoserine1 Publication

Post-translational modificationi

Extensively phosphorylated on serine residues in the C-terminal region By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ62376.
PaxDbiQ62376.
PRIDEiQ62376.

PTM databases

PhosphoSiteiQ62376.

Expressioni

Gene expression databases

ArrayExpressiQ62376.
BgeeiQ62376.
GenevestigatoriQ62376.

Interactioni

Subunit structurei

U1 snRNP is composed of the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in an heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. Interacts with SCNM1. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated SFRS13A and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SCAF11, and ZRANB2.1 Publication

Protein-protein interaction databases

BioGridi203376. 2 interactions.
IntActiQ62376. 2 interactions.
MINTiMINT-1868298.

Structurei

3D structure databases

ProteinModelPortaliQ62376.
SMRiQ62376. Positions 114-177.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini103 – 18179RRMAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi231 – 31080Arg/Glu-rich (mixed charge)Add
BLAST
Compositional biasi311 – 32616Poly-GlyAdd
BLAST
Compositional biasi356 – 40348Arg/Asp/Glu-rich (mixed charge)Add
BLAST
Compositional biasi404 – 4096Poly-Gly

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0724.
GeneTreeiENSGT00530000063750.
HOGENOMiHOG000236289.
HOVERGENiHBG094947.
InParanoidiQ62376.
KOiK11093.
OMAiDGRDMYM.
OrthoDBiEOG79W96V.
PhylomeDBiQ62376.
TreeFamiTF314215.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR022023. U1snRNP70_N.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
PF12220. U1snRNP70_N. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q62376-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTQFLPPNLL ALFAPRDPIP YLPPLEKLPH EKHHNQPYCG IAPYIREFED    50
PRDAPPPTRA ETREERMERK RREKIERRQQ EVETELKMWD PHNDPNAQGD 100
AFKTLFVARV NYDTTESKLR REFEVYGPIK RIHMVYSKRS GKPRGYAFIE 150
YEHERDMHSA YKHADGKKID GRRVLVDVER GRTVKGWRPR RLGGGLGGTR 200
RGGADVNIRH SGRDDTSRYD ERPGPSPLPH RDRDRDRERE RRERSRERDK 250
ERERRRSRSR DRRRRSRSRD KDERRRSRER SKDKDRDRKR RSSRSRERAR 300
RERERKEELR GGGGGGGGGS GGGGGGDMAE PSEAGDGAPD DGPPGELGPE 350
GPDGPEEKGR DRDRERRRSH RSERERRRDR DRDRDREHKR GERGSERGRD 400
EARGGGGSGQ DNGLEGLGSD GRDMYMEAEG GDGYMAPENG YLMEAAPE 448
Length:448
Mass (Da):51,992
Last modified:December 6, 2005 - v2
Checksum:i5B025A3B6992D0BD
GO
Isoform 2 (identifier: Q62376-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     160-166: AYKHADG → TTQLACS
     167-448: Missing.

Show »
Length:166
Mass (Da):19,790
Checksum:iE90F034A08D3E79C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei160 – 1667AYKHADG → TTQLACS in isoform 2. VSP_005851
Alternative sequencei167 – 448282Missing in isoform 2. VSP_005852Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti263 – 2631R → Q in CAA33777. 1 Publication
Sequence conflicti281 – 2811S → T in CAA33777. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK133115 mRNA. Translation: BAE21515.1.
AK146729 mRNA. Translation: BAE27392.1.
X15769
, X15770, X15771, X15772, X15774, X15775, X15776 Genomic DNA. Translation: CAA33777.1.
CCDSiCCDS21240.1. [Q62376-1]
PIRiS04336.
S04824.
RefSeqiNP_033250.3. NM_009224.4. [Q62376-1]
XP_006540795.1. XM_006540732.1. [Q62376-1]
UniGeneiMm.216386.

Genome annotation databases

EnsembliENSMUST00000074575; ENSMUSP00000074160; ENSMUSG00000063511. [Q62376-1]
GeneIDi20637.
KEGGimmu:20637.
UCSCiuc009guw.1. mouse. [Q62376-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK133115 mRNA. Translation: BAE21515.1 .
AK146729 mRNA. Translation: BAE27392.1 .
X15769
, X15770 , X15771 , X15772 , X15774 , X15775 , X15776 Genomic DNA. Translation: CAA33777.1 .
CCDSi CCDS21240.1. [Q62376-1 ]
PIRi S04336.
S04824.
RefSeqi NP_033250.3. NM_009224.4. [Q62376-1 ]
XP_006540795.1. XM_006540732.1. [Q62376-1 ]
UniGenei Mm.216386.

3D structure databases

ProteinModelPortali Q62376.
SMRi Q62376. Positions 114-177.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203376. 2 interactions.
IntActi Q62376. 2 interactions.
MINTi MINT-1868298.

PTM databases

PhosphoSitei Q62376.

Proteomic databases

MaxQBi Q62376.
PaxDbi Q62376.
PRIDEi Q62376.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000074575 ; ENSMUSP00000074160 ; ENSMUSG00000063511 . [Q62376-1 ]
GeneIDi 20637.
KEGGi mmu:20637.
UCSCi uc009guw.1. mouse. [Q62376-1 ]

Organism-specific databases

CTDi 6625.
MGIi MGI:98341. Snrnp70.

Phylogenomic databases

eggNOGi COG0724.
GeneTreei ENSGT00530000063750.
HOGENOMi HOG000236289.
HOVERGENi HBG094947.
InParanoidi Q62376.
KOi K11093.
OMAi DGRDMYM.
OrthoDBi EOG79W96V.
PhylomeDBi Q62376.
TreeFami TF314215.

Miscellaneous databases

ChiTaRSi SNRNP70. mouse.
NextBioi 299035.
PROi Q62376.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q62376.
Bgeei Q62376.
Genevestigatori Q62376.

Family and domain databases

Gene3Di 3.30.70.330. 1 hit.
InterProi IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR022023. U1snRNP70_N.
[Graphical view ]
Pfami PF00076. RRM_1. 1 hit.
PF12220. U1snRNP70_N. 1 hit.
[Graphical view ]
SMARTi SM00360. RRM. 1 hit.
[Graphical view ]
PROSITEi PS50102. RRM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Kidney and Testis.
  2. "Analysis of genomic clones of the murine U1RNA-associated 70-kDa protein reveals a high evolutionary conservation of the protein between human and mouse."
    Hornig H., Fischer U., Costas M., Rauh A., Luehrmann R.
    Eur. J. Biochem. 182:45-50(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-448 (ISOFORMS 1 AND 2).
    Strain: BALB/c.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. "Evidence for a direct role of the disease modifier SCNM1 in splicing."
    Howell V.M., Jones J.M., Bergren S.K., Li L., Billi A.C., Avenarius M.R., Meisler M.H.
    Hum. Mol. Genet. 16:2506-2516(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SCNM1, SUBCELLULAR LOCATION.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408 AND SER-419, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiRU17_MOUSE
AccessioniPrimary (citable) accession number: Q62376
Secondary accession number(s): Q3UIW4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: December 6, 2005
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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