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Q62351

- TFR1_MOUSE

UniProt

Q62351 - TFR1_MOUSE

Protein

Transferrin receptor protein 1

Gene

Tfrc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system By similarity.By similarity

    GO - Molecular functioni

    1. double-stranded RNA binding Source: MGI
    2. iron ion transmembrane transporter activity Source: MGI
    3. peptidase activity Source: InterPro
    4. protein binding Source: MGI
    5. transferrin receptor activity Source: MGI

    GO - Biological processi

    1. cellular iron ion homeostasis Source: MGI
    2. iron ion transmembrane transport Source: GOC
    3. osteoclast differentiation Source: DFLAT
    4. positive regulation of bone resorption Source: DFLAT
    5. receptor-mediated endocytosis Source: GOC

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Endocytosis

    Enzyme and pathway databases

    ReactomeiREACT_198515. Transferrin endocytosis and recycling.

    Protein family/group databases

    MEROPSiM28.972.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transferrin receptor protein 1
    Short name:
    TR
    Short name:
    TfR
    Short name:
    TfR1
    Short name:
    Trfr
    Alternative name(s):
    CD_antigen: CD71
    Gene namesi
    Name:Tfrc
    Synonyms:Trfr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:98822. Tfrc.

    Subcellular locationi

    Cell membrane By similarity; Single-pass type II membrane protein By similarity. Melanosome By similarity

    GO - Cellular componenti

    1. cell surface Source: MGI
    2. coated pit Source: MGI
    3. cytoplasmic membrane-bounded vesicle Source: MGI
    4. endosome Source: MGI
    5. external side of plasma membrane Source: MGI
    6. extracellular vesicular exosome Source: MGI
    7. integral component of membrane Source: UniProtKB-KW
    8. melanosome Source: UniProtKB-SubCell
    9. perinuclear region of cytoplasm Source: MGI
    10. plasma membrane Source: MGI
    11. recycling endosome Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 763763Transferrin receptor protein 1PRO_0000174133Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei20 – 201Phosphotyrosine2 Publications
    Modified residuei21 – 211PhosphothreonineBy similarity
    Modified residuei24 – 241PhosphoserineBy similarity
    Lipidationi67 – 671S-palmitoyl cysteineBy similarity
    Disulfide bondi89 – 89InterchainBy similarity
    Disulfide bondi98 – 98InterchainBy similarity
    Glycosylationi104 – 1041O-linked (GalNAc...)By similarity
    Glycosylationi253 – 2531N-linked (GlcNAc...)By similarity
    Glycosylationi319 – 3191N-linked (GlcNAc...)By similarity
    Glycosylationi725 – 7251N-linked (GlcNAc...)1 Publication
    Glycosylationi730 – 7301N-linked (GlcNAc...)1 Publication

    Post-translational modificationi

    N- and O-glycosylated, phosphorylated and palmitoylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiQ62351.
    PaxDbiQ62351.
    PRIDEiQ62351.

    PTM databases

    PhosphoSiteiQ62351.

    Expressioni

    Gene expression databases

    ArrayExpressiQ62351.
    BgeeiQ62351.
    CleanExiMM_TFRC.
    GenevestigatoriQ62351.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Binds one transferrin molecule per subunit. Interacts with SH3BP4 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi204314. 2 interactions.
    IntActiQ62351. 3 interactions.
    MINTiMINT-1858479.

    Structurei

    3D structure databases

    ProteinModelPortaliQ62351.
    SMRiQ62351. Positions 123-762.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6767CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini89 – 763675ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei68 – 8821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini225 – 31591PAAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 6767Mediates interaction with SH3BP4By similarityAdd
    BLAST
    Regioni572 – 763192Ligand-bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi20 – 234Endocytosis signal
    Motifi58 – 614Stop-transfer sequence
    Motifi649 – 6513Cell attachment siteSequence Analysis

    Sequence similaritiesi

    Belongs to the peptidase M28 family. M28B subfamily.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG74799.
    GeneTreeiENSGT00550000074421.
    HOGENOMiHOG000124067.
    HOVERGENiHBG023177.
    InParanoidiQ62351.
    KOiK06503.
    OMAiDNSHVEM.
    OrthoDBiEOG780RKW.
    PhylomeDBiQ62351.
    TreeFamiTF312981.

    Family and domain databases

    Gene3Di1.20.930.40. 1 hit.
    InterProiIPR007484. Peptidase_M28.
    IPR003137. Protease-assoc_domain.
    IPR029513. TfR.
    IPR007365. TFR-like_dimer_dom.
    [Graphical view]
    PANTHERiPTHR10404:SF26. PTHR10404:SF26. 1 hit.
    PfamiPF02225. PA. 1 hit.
    PF04389. Peptidase_M28. 1 hit.
    PF04253. TFR_dimer. 1 hit.
    [Graphical view]
    SUPFAMiSSF47672. SSF47672. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q62351-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMDQARSAFS NLFGGEPLSY TRFSLARQVD GDNSHVEMKL AADEEENADN    50
    NMKASVRKPK RFNGRLCFAA IALVIFFLIG FMSGYLGYCK RVEQKEECVK 100
    LAETEETDKS ETMETEDVPT SSRLYWADLK TLLSEKLNSI EFADTIKQLS 150
    QNTYTPREAG SQKDESLAYY IENQFHEFKF SKVWRDEHYV KIQVKSSIGQ 200
    NMVTIVQSNG NLDPVESPEG YVAFSKPTEV SGKLVHANFG TKKDFEELSY 250
    SVNGSLVIVR AGEITFAEKV ANAQSFNAIG VLIYMDKNKF PVVEADLALF 300
    GHAHLGTGDP YTPGFPSFNH TQFPPSQSSG LPNIPVQTIS RAAAEKLFGK 350
    MEGSCPARWN IDSSCKLELS QNQNVKLIVK NVLKERRILN IFGVIKGYEE 400
    PDRYVVVGAQ RDALGAGVAA KSSVGTGLLL KLAQVFSDMI SKDGFRPSRS 450
    IIFASWTAGD FGAVGATEWL EGYLSSLHLK AFTYINLDKV VLGTSNFKVS 500
    ASPLLYTLMG KIMQDVKHPV DGKSLYRDSN WISKVEKLSF DNAAYPFLAY 550
    SGIPAVSFCF CEDADYPYLG TRLDTYEALT QKVPQLNQMV RTAAEVAGQL 600
    IIKLTHDVEL NLDYEMYNSK LLSFMKDLNQ FKTDIRDMGL SLQWLYSARG 650
    DYFRATSRLT TDFHNAEKTN RFVMREINDR IMKVEYHFLS PYVSPRESPF 700
    RHIFWGSGSH TLSALVENLK LRQKNITAFN ETLFRNQLAL ATWTIQGVAN 750
    ALSGDIWNID NEF 763
    Length:763
    Mass (Da):85,731
    Last modified:November 1, 1996 - v1
    Checksum:i832A148CC26CE489
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 262LA → AL in AAA37616. (PubMed:2984291)Curated
    Sequence conflicti743 – 7431W → H AA sequence (PubMed:6092468)Curated
    Sequence conflicti757 – 7571W → I AA sequence (PubMed:6092468)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57349 mRNA. Translation: CAA40624.1.
    BC054522 mRNA. Translation: AAH54522.1.
    M29618 mRNA. Translation: AAA37616.1.
    CCDSiCCDS28123.1.
    PIRiS29548.
    RefSeqiNP_035768.1. NM_011638.4.
    XP_006522062.1. XM_006521999.1.
    UniGeneiMm.28683.

    Genome annotation databases

    EnsembliENSMUST00000023486; ENSMUSP00000023486; ENSMUSG00000022797.
    GeneIDi22042.
    KEGGimmu:22042.
    UCSCiuc007yza.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57349 mRNA. Translation: CAA40624.1 .
    BC054522 mRNA. Translation: AAH54522.1 .
    M29618 mRNA. Translation: AAA37616.1 .
    CCDSi CCDS28123.1.
    PIRi S29548.
    RefSeqi NP_035768.1. NM_011638.4.
    XP_006522062.1. XM_006521999.1.
    UniGenei Mm.28683.

    3D structure databases

    ProteinModelPortali Q62351.
    SMRi Q62351. Positions 123-762.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204314. 2 interactions.
    IntActi Q62351. 3 interactions.
    MINTi MINT-1858479.

    Protein family/group databases

    MEROPSi M28.972.

    PTM databases

    PhosphoSitei Q62351.

    Proteomic databases

    MaxQBi Q62351.
    PaxDbi Q62351.
    PRIDEi Q62351.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023486 ; ENSMUSP00000023486 ; ENSMUSG00000022797 .
    GeneIDi 22042.
    KEGGi mmu:22042.
    UCSCi uc007yza.2. mouse.

    Organism-specific databases

    CTDi 7037.
    MGIi MGI:98822. Tfrc.

    Phylogenomic databases

    eggNOGi NOG74799.
    GeneTreei ENSGT00550000074421.
    HOGENOMi HOG000124067.
    HOVERGENi HBG023177.
    InParanoidi Q62351.
    KOi K06503.
    OMAi DNSHVEM.
    OrthoDBi EOG780RKW.
    PhylomeDBi Q62351.
    TreeFami TF312981.

    Enzyme and pathway databases

    Reactomei REACT_198515. Transferrin endocytosis and recycling.

    Miscellaneous databases

    NextBioi 301828.
    PROi Q62351.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q62351.
    Bgeei Q62351.
    CleanExi MM_TFRC.
    Genevestigatori Q62351.

    Family and domain databases

    Gene3Di 1.20.930.40. 1 hit.
    InterProi IPR007484. Peptidase_M28.
    IPR003137. Protease-assoc_domain.
    IPR029513. TfR.
    IPR007365. TFR-like_dimer_dom.
    [Graphical view ]
    PANTHERi PTHR10404:SF26. PTHR10404:SF26. 1 hit.
    Pfami PF02225. PA. 1 hit.
    PF04389. Peptidase_M28. 1 hit.
    PF04253. TFR_dimer. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47672. SSF47672. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Trowbridge I.S., Domingo D.L., Thomas M.L., Chain A.
      Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X DBA/2.
      Tissue: Hematopoietic.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "cDNA cloning of the murine transferrin receptor: sequence of trans-membrane and adjacent regions."
      Stearne P.A., Pietersz G.A., Goding J.W.
      J. Immunol. 134:3474-3479(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-301.
      Tissue: Myeloma.
    4. "The receptor for transferrin on murine myeloma cells: one-step purification based on its physiology, and partial amino acid sequence."
      van Driel I.R., Stearne P.A., Grego B., Simpson R.J., Goding J.W.
      J. Immunol. 133:3220-3224(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 7-19; 158-179; 196-208; 450-467 AND 736-759.
      Tissue: Myeloma.
    5. "Transferrin receptor is necessary for development of erythrocytes and the nervous system."
      Levy J.E., Jin O., Fujiwara Y., Kuo F., Andrews N.C.
      Nat. Genet. 21:396-399(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-20, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.
    7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-20, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    8. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-725 AND ASN-730.

    Entry informationi

    Entry nameiTFR1_MOUSE
    AccessioniPrimary (citable) accession number: Q62351
    Secondary accession number(s): Q61560
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 11, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3