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Q62351 (TFR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transferrin receptor protein 1

Short name=TR
Short name=TfR
Short name=TfR1
Short name=Trfr
Alternative name(s):
CD_antigen=CD71
Gene names
Name:Tfrc
Synonyms:Trfr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length763 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system By similarity. Ref.5

Subunit structure

Homodimer; disulfide-linked. Binds one transferrin molecule per subunit. Interacts with SH3BP4 By similarity.

Subcellular location

Cell membrane; Single-pass type II membrane protein By similarity. Melanosome By similarity.

Post-translational modification

N- and O-glycosylated, phosphorylated and palmitoylated By similarity.

Sequence similarities

Belongs to the peptidase M28 family. M28B subfamily.

Contains 1 PA (protease associated) domain.

Ontologies

Keywords
   Biological processEndocytosis
   Cellular componentCell membrane
Membrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular iron ion homeostasis

Inferred from mutant phenotype Ref.5. Source: MGI

iron ion transmembrane transport

Traceable author statement Ref.5. Source: GOC

osteoclast differentiation

Inferred from mutant phenotype PubMed 19252502. Source: DFLAT

positive regulation of bone resorption

Inferred from direct assay PubMed 19252502. Source: DFLAT

receptor-mediated endocytosis

Traceable author statement Ref.5. Source: GOC

   Cellular_componentcell surface

Inferred from direct assay PubMed 23416069. Source: MGI

coated pit

Inferred from electronic annotation. Source: Ensembl

cytoplasmic membrane-bounded vesicle

Inferred from sequence orthology PubMed 15229288. Source: MGI

endosome

Inferred from direct assay PubMed 14562105PubMed 14668490PubMed 14718562PubMed 16227996PubMed 18321981PubMed 23303939. Source: MGI

external side of plasma membrane

Inferred from direct assay PubMed 20424607PubMed 20711497. Source: MGI

extracellular vesicular exosome

Inferred from direct assay PubMed 19724054. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

perinuclear region of cytoplasm

Inferred from direct assay PubMed 10444069. Source: MGI

plasma membrane

Inferred from direct assay PubMed 10444069PubMed 14562105PubMed 16227996PubMed 23303939. Source: MGI

recycling endosome

Inferred from direct assay PubMed 23395379. Source: MGI

   Molecular_functiondouble-stranded RNA binding

Inferred from sequence orthology PubMed 21266579. Source: MGI

iron ion transmembrane transporter activity

Traceable author statement Ref.5. Source: MGI

peptidase activity

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction PubMed 23416069. Source: MGI

transferrin receptor activity

Traceable author statement Ref.5. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 763763Transferrin receptor protein 1
PRO_0000174133

Regions

Topological domain1 – 6767Cytoplasmic Potential
Transmembrane68 – 8821Helical; Signal-anchor for type II membrane protein; Potential
Topological domain89 – 763675Extracellular Potential
Domain225 – 31591PA
Region1 – 6767Mediates interaction with SH3BP4 By similarity
Region572 – 763192Ligand-binding By similarity
Motif20 – 234Endocytosis signal
Motif58 – 614Stop-transfer sequence
Motif649 – 6513Cell attachment site Potential

Amino acid modifications

Modified residue201Phosphotyrosine Ref.6 Ref.7
Modified residue211Phosphothreonine By similarity
Modified residue241Phosphoserine By similarity
Lipidation671S-palmitoyl cysteine By similarity
Glycosylation1041O-linked (GalNAc...) By similarity
Glycosylation2531N-linked (GlcNAc...) By similarity
Glycosylation3191N-linked (GlcNAc...) By similarity
Glycosylation7251N-linked (GlcNAc...) Ref.8
Glycosylation7301N-linked (GlcNAc...) Ref.8
Disulfide bond89Interchain By similarity
Disulfide bond98Interchain By similarity

Experimental info

Sequence conflict25 – 262LA → AL in AAA37616. Ref.3
Sequence conflict7431W → H AA sequence Ref.4
Sequence conflict7571W → I AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q62351 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 832A148CC26CE489

FASTA76385,731
        10         20         30         40         50         60 
MMDQARSAFS NLFGGEPLSY TRFSLARQVD GDNSHVEMKL AADEEENADN NMKASVRKPK 

        70         80         90        100        110        120 
RFNGRLCFAA IALVIFFLIG FMSGYLGYCK RVEQKEECVK LAETEETDKS ETMETEDVPT 

       130        140        150        160        170        180 
SSRLYWADLK TLLSEKLNSI EFADTIKQLS QNTYTPREAG SQKDESLAYY IENQFHEFKF 

       190        200        210        220        230        240 
SKVWRDEHYV KIQVKSSIGQ NMVTIVQSNG NLDPVESPEG YVAFSKPTEV SGKLVHANFG 

       250        260        270        280        290        300 
TKKDFEELSY SVNGSLVIVR AGEITFAEKV ANAQSFNAIG VLIYMDKNKF PVVEADLALF 

       310        320        330        340        350        360 
GHAHLGTGDP YTPGFPSFNH TQFPPSQSSG LPNIPVQTIS RAAAEKLFGK MEGSCPARWN 

       370        380        390        400        410        420 
IDSSCKLELS QNQNVKLIVK NVLKERRILN IFGVIKGYEE PDRYVVVGAQ RDALGAGVAA 

       430        440        450        460        470        480 
KSSVGTGLLL KLAQVFSDMI SKDGFRPSRS IIFASWTAGD FGAVGATEWL EGYLSSLHLK 

       490        500        510        520        530        540 
AFTYINLDKV VLGTSNFKVS ASPLLYTLMG KIMQDVKHPV DGKSLYRDSN WISKVEKLSF 

       550        560        570        580        590        600 
DNAAYPFLAY SGIPAVSFCF CEDADYPYLG TRLDTYEALT QKVPQLNQMV RTAAEVAGQL 

       610        620        630        640        650        660 
IIKLTHDVEL NLDYEMYNSK LLSFMKDLNQ FKTDIRDMGL SLQWLYSARG DYFRATSRLT 

       670        680        690        700        710        720 
TDFHNAEKTN RFVMREINDR IMKVEYHFLS PYVSPRESPF RHIFWGSGSH TLSALVENLK 

       730        740        750        760 
LRQKNITAFN ETLFRNQLAL ATWTIQGVAN ALSGDIWNID NEF 

« Hide

References

« Hide 'large scale' references
[1]Trowbridge I.S., Domingo D.L., Thomas M.L., Chain A.
Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X DBA/2.
Tissue: Hematopoietic.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"cDNA cloning of the murine transferrin receptor: sequence of trans-membrane and adjacent regions."
Stearne P.A., Pietersz G.A., Goding J.W.
J. Immunol. 134:3474-3479(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-301.
Tissue: Myeloma.
[4]"The receptor for transferrin on murine myeloma cells: one-step purification based on its physiology, and partial amino acid sequence."
van Driel I.R., Stearne P.A., Grego B., Simpson R.J., Goding J.W.
J. Immunol. 133:3220-3224(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 7-19; 158-179; 196-208; 450-467 AND 736-759.
Tissue: Myeloma.
[5]"Transferrin receptor is necessary for development of erythrocytes and the nervous system."
Levy J.E., Jin O., Fujiwara Y., Kuo F., Andrews N.C.
Nat. Genet. 21:396-399(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-20, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[7]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-20, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[8]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-725 AND ASN-730.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57349 mRNA. Translation: CAA40624.1.
BC054522 mRNA. Translation: AAH54522.1.
M29618 mRNA. Translation: AAA37616.1.
CCDSCCDS28123.1.
PIRS29548.
RefSeqNP_035768.1. NM_011638.4.
XP_006522062.1. XM_006521999.1.
UniGeneMm.28683.

3D structure databases

ProteinModelPortalQ62351.
SMRQ62351. Positions 123-762.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204314. 2 interactions.
IntActQ62351. 3 interactions.
MINTMINT-1858479.

Protein family/group databases

MEROPSM28.972.

PTM databases

PhosphoSiteQ62351.

Proteomic databases

MaxQBQ62351.
PaxDbQ62351.
PRIDEQ62351.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023486; ENSMUSP00000023486; ENSMUSG00000022797.
GeneID22042.
KEGGmmu:22042.
UCSCuc007yza.2. mouse.

Organism-specific databases

CTD7037.
MGIMGI:98822. Tfrc.

Phylogenomic databases

eggNOGNOG74799.
GeneTreeENSGT00550000074421.
HOGENOMHOG000124067.
HOVERGENHBG023177.
InParanoidQ62351.
KOK06503.
OMADNSHVEM.
OrthoDBEOG780RKW.
PhylomeDBQ62351.
TreeFamTF312981.

Gene expression databases

ArrayExpressQ62351.
BgeeQ62351.
CleanExMM_TFRC.
GenevestigatorQ62351.

Family and domain databases

Gene3D1.20.930.40. 1 hit.
InterProIPR007484. Peptidase_M28.
IPR003137. Protease-assoc_domain.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamPF02225. PA. 1 hit.
PF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMSSF47672. SSF47672. 1 hit.
ProtoNetSearch...

Other

NextBio301828.
PROQ62351.
SOURCESearch...

Entry information

Entry nameTFR1_MOUSE
AccessionPrimary (citable) accession number: Q62351
Secondary accession number(s): Q61560
Entry history
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot