Q62351 (TFR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transferrin receptor protein 1
Short name=TR
Short name=TfR
Short name=TfR1
Short name=Trfr

Alternative name(s):
CD_antigen=CD71

Gene names

Name:	Tfrc
Synonyms:	Trfr

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system By similarity. Ref.5
Subunit structure	Homodimer; disulfide-linked. Binds one transferrin molecule per subunit. Interacts with SH3BP4 By similarity.
Subcellular location	Cell membrane; Single-pass type II membrane protein By similarity. Melanosome By similarity.
Post-translational modification	N- and O-glycosylated, phosphorylated and palmitoylated By similarity.
Sequence similarities	Belongs to the peptidase M28 family. M28B subfamily. Contains 1 PA (protease associated) domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from mutant phenotype Ref.5. Source: MGI osteoclast differentiation Inferred from mutant phenotype PubMed 19252502. Source: DFLAT positive regulation of bone resorption Inferred from direct assay PubMed 19252502. Source: DFLAT proteolysis Inferred from electronic annotation. Source: InterPro
Cellular_component	coated pit Inferred from electronic annotation. Source: Compara cytoplasmic membrane-bounded vesicle Inferred from sequence orthology PubMed 15229288. Source: MGI endosome Inferred from direct assay PubMed 14562105PubMed 14668490PubMed 14718562PubMed 16227996PubMed 18321981. Source: MGI external side of plasma membrane Inferred from direct assay PubMed 20424607PubMed 20711497. Source: MGI extracellular vesicular exosome Inferred from direct assay PubMed 19724054. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from direct assay PubMed 10444069. Source: MGI
Molecular_function	iron ion transmembrane transporter activity Traceable author statement Ref.5. Source: MGI peptidase activity Inferred from electronic annotation. Source: InterPro transferrin receptor activity Traceable author statement Ref.5. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 763	763	Transferrin receptor protein 1		PRO_0000174133
Regions
	Topological domain	1 – 67	67	Cytoplasmic Potential
	Transmembrane	68 – 88	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	89 – 763	675	Extracellular Potential
	Domain	225 – 315	91	PA
	Region	1 – 67	67	Mediates interaction with SH3BP4 By similarity
	Region	572 – 763	192	Ligand-binding By similarity
	Motif	20 – 23	4	Endocytosis signal
	Motif	58 – 61	4	Stop-transfer sequence
	Motif	649 – 651	3	Cell attachment site Potential
Amino acid modifications
	Modified residue	20	1	Phosphotyrosine Ref.6 Ref.7
	Modified residue	21	1	Phosphothreonine By similarity
	Modified residue	24	1	Phosphoserine By similarity
	Lipidation	67	1	S-palmitoyl cysteine By similarity
	Glycosylation	104	1	O-linked (GalNAc...) By similarity
	Glycosylation	253	1	N-linked (GlcNAc...) By similarity
	Glycosylation	319	1	N-linked (GlcNAc...) By similarity
	Glycosylation	725	1	N-linked (GlcNAc...) Ref.8
	Glycosylation	730	1	N-linked (GlcNAc...) Ref.8
	Disulfide bond	89		Interchain By similarity
	Disulfide bond	98		Interchain By similarity
Experimental info
	Sequence conflict	25 – 26	2	LA → AL in AAA37616. Ref.3
	Sequence conflict	743	1	W → H AA sequence Ref.4
	Sequence conflict	757	1	W → I AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q62351 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 832A148CC26CE489	FASTA	763	85,731
	10 20 30 40 50 60 MMDQARSAFS NLFGGEPLSY TRFSLARQVD GDNSHVEMKL AADEEENADN NMKASVRKPK 70 80 90 100 110 120 RFNGRLCFAA IALVIFFLIG FMSGYLGYCK RVEQKEECVK LAETEETDKS ETMETEDVPT 130 140 150 160 170 180 SSRLYWADLK TLLSEKLNSI EFADTIKQLS QNTYTPREAG SQKDESLAYY IENQFHEFKF 190 200 210 220 230 240 SKVWRDEHYV KIQVKSSIGQ NMVTIVQSNG NLDPVESPEG YVAFSKPTEV SGKLVHANFG 250 260 270 280 290 300 TKKDFEELSY SVNGSLVIVR AGEITFAEKV ANAQSFNAIG VLIYMDKNKF PVVEADLALF 310 320 330 340 350 360 GHAHLGTGDP YTPGFPSFNH TQFPPSQSSG LPNIPVQTIS RAAAEKLFGK MEGSCPARWN 370 380 390 400 410 420 IDSSCKLELS QNQNVKLIVK NVLKERRILN IFGVIKGYEE PDRYVVVGAQ RDALGAGVAA 430 440 450 460 470 480 KSSVGTGLLL KLAQVFSDMI SKDGFRPSRS IIFASWTAGD FGAVGATEWL EGYLSSLHLK 490 500 510 520 530 540 AFTYINLDKV VLGTSNFKVS ASPLLYTLMG KIMQDVKHPV DGKSLYRDSN WISKVEKLSF 550 560 570 580 590 600 DNAAYPFLAY SGIPAVSFCF CEDADYPYLG TRLDTYEALT QKVPQLNQMV RTAAEVAGQL 610 620 630 640 650 660 IIKLTHDVEL NLDYEMYNSK LLSFMKDLNQ FKTDIRDMGL SLQWLYSARG DYFRATSRLT 670 680 690 700 710 720 TDFHNAEKTN RFVMREINDR IMKVEYHFLS PYVSPRESPF RHIFWGSGSH TLSALVENLK 730 740 750 760 LRQKNITAFN ETLFRNQLAL ATWTIQGVAN ALSGDIWNID NEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Trowbridge I.S., Domingo D.L., Thomas M.L., Chain A. Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X DBA/2. Tissue: Hematopoietic.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"cDNA cloning of the murine transferrin receptor: sequence of trans-membrane and adjacent regions." Stearne P.A., Pietersz G.A., Goding J.W. J. Immunol. 134:3474-3479(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-301. Tissue: Myeloma.
[4]	"The receptor for transferrin on murine myeloma cells: one-step purification based on its physiology, and partial amino acid sequence." van Driel I.R., Stearne P.A., Grego B., Simpson R.J., Goding J.W. J. Immunol. 133:3220-3224(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 7-19; 158-179; 196-208; 450-467 AND 736-759. Tissue: Myeloma.
[5]	"Transferrin receptor is necessary for development of erythrocytes and the nervous system." Levy J.E., Jin O., Fujiwara Y., Kuo F., Andrews N.C. Nat. Genet. 21:396-399(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-20, MASS SPECTROMETRY. Tissue: Mast cell.
[7]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-20, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
[8]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-725 AND ASN-730, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57349 mRNA. Translation: CAA40624.1. BC054522 mRNA. Translation: AAH54522.1. M29618 mRNA. Translation: AAA37616.1.
IPI	IPI00124700.
PIR	S29548.
RefSeq	NP_035768.1. NM_011638.4.
UniGene	Mm.28683.
3D structure databases
ProteinModelPortal	Q62351.
SMR	Q62351. Positions 123-762.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62351. 1 interaction.
Protein family/group databases
MEROPS	M28.972.
PTM databases
PhosphoSite	Q62351.
Proteomic databases
PaxDb	Q62351.
PRIDE	Q62351.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023486; ENSMUSP00000023486; ENSMUSG00000022797.
GeneID	22042.
KEGG	mmu:22042.
Organism-specific databases
CTD	7037.
MGI	MGI:98822. Tfrc.
Phylogenomic databases
eggNOG	NOG74799.
GeneTree	ENSGT00550000074421.
HOGENOM	HOG000124067.
HOVERGEN	HBG023177.
InParanoid	Q62351.
KO	K06503.
OMA	YVAYSKA.
OrthoDB	EOG41C6VQ.
Gene expression databases
ArrayExpress	Q62351.
Bgee	Q62351.
CleanEx	MM_TFRC.
Genevestigator	Q62351.
GermOnline	ENSMUSG00000022797. Mus musculus.
Family and domain databases
Gene3D	1.20.930.40. 1 hit.
InterPro	IPR007484. Peptidase_M28. IPR003137. Protease-assoc_domain. IPR007365. TFR-like_dimer_dom. [Graphical view]
Pfam	PF02225. PA. 1 hit. PF04389. Peptidase_M28. 1 hit. PF04253. TFR_dimer. 1 hit. [Graphical view]
SUPFAM	SSF47672. Transferrin_rcpt-like_dimerise. 1 hit.
ProtoNet	Search...
Other
NextBio	301828.
SOURCE	Search...

Entry information

Entry name

TFR1_MOUSE

Accession

Primary (citable) accession number: Q62351
Secondary accession number(s): Q61560

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program