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Reviewed, UniProtKB/Swiss-Prot Q62312 (TGFR2_MOUSE)

Last modified November 25, 2008. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    TGF-beta receptor type-2
    EC=2.7.11.30
Alternative name(s):
    Transforming growth factor-beta receptor type II
      Short name=TGF-beta receptor type II
    TGF-beta type II receptor
    TbetaR-II
    TGFR-2
Gene names
Name: Tgfbr2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length592 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta.

Catalytic activity

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Cofactor

Magnesium or manganese By similarity.

Subunit structure

Binds to DAXX. Interacts with TCTEX1D4 By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Widely expressed in adult. Expressed primarily in mesenchyme and epidermis of the midgestational fetus.

Post-translational modification

Phosphorylated on a Ser/Thr residue in the cytoplasmic domain By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords

   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
   LigandATP-binding
Magnesium
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Receptor
Serine/threonine-protein kinase
Transferase
   PTMGlycoprotein
Phosphoprotein

Gene Ontology (GO)

   Biological processactivation of protein kinase activity

Inferred from direct assay. Source: MGI

brain development

Inferred from mutant phenotype. Source: UniProtKB

embryonic cranial skeleton morphogenesis

Inferred from mutant phenotype. Source: MGI

embryonic hemopoiesis

Inferred from mutant phenotype. Source: UniProtKB

heart development

Inferred from mutant phenotype. Source: MGI

myeloid dendritic cell differentiation

Inferred from mutant phenotype. Source: MGI

palate development

Inferred from mutant phenotype. Source: UniProtKB

patterning of blood vessels

Inferred from mutant phenotype. Source: MGI

positive regulation of B cell tolerance induction

Inferred from mutant phenotype. Source: MGI

positive regulation of NK T cell differentiation

Inferred from mutant phenotype. Source: MGI

positive regulation of T cell tolerance induction

Inferred from mutant phenotype. Source: MGI

positive regulation of mesenchymal cell proliferation

Inferred from mutant phenotype. Source: UniProtKB

positive regulation of tolerance induction to self antigen

Inferred from mutant phenotype. Source: MGI

protein amino acid phosphorylation

Traceable author statement. Source: MGI

transmembrane receptor protein serine/threonine kinase signaling pathway

Inferred from electronic annotation. Source: InterPro

vasculogenesis

Inferred from mutant phenotype. Source: UniProtKB

   Cellular componentexternal side of plasma membrane

Inferred from direct assay. Source: MGI

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: InterPro

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction. Source: UniProtKB

receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

receptor signaling protein serine/threonine kinase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform RII-2 (identifier: Q62312-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform RII-1 (identifier: Q62312-2)

The sequence of this isoform differs from the canonical sequence as follows:
     32-56: Missing.
     57-57: F → V

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 592569TGF-beta receptor type-2
PRO_0000024427

Regions

Topological domain24 – 184161Extracellular Potential
Transmembrane185 – 21430 Potential
Topological domain215 – 592378Cytoplasmic Potential
Domain269 – 569301Protein kinase
Nucleotide binding275 – 2839ATP By similarity

Sites

Active site4041Proton acceptor By similarity
Binding site3021ATP By similarity

Amino acid modifications

Modified residue5731Phosphoserine By similarity
Glycosylation481N-linked (GlcNAc...) Potential
Glycosylation951N-linked (GlcNAc...) Potential
Glycosylation1191N-linked (GlcNAc...) Potential
Disulfide bond76 ↔ 109 By similarity
Disulfide bond79 ↔ 96 By similarity
Disulfide bond86 ↔ 92 By similarity
Disulfide bond102 ↔ 126 By similarity
Disulfide bond146 ↔ 161 By similarity
Disulfide bond163 ↔ 168 By similarity

Natural variations

Alternative sequence32 – 5625Missing in isoform RII-1.
VSP_004955
Alternative sequence571F → V in isoform RII-1.
VSP_004956

Experimental info

Sequence conflict81G → A in AAB30100. Ref.2
Sequence conflict1171D → N in AAB30100. Ref.2
Sequence conflict3541A → R in AAB30100. Ref.2
Sequence conflict440 – 4434NSGQ → KQRE in AAB30100. Ref.2
Sequence conflict4861C → W in AAB30100. Ref.2
Sequence conflict5061H → P in AAB30100. Ref.2
Sequence conflict569 – 5702PE → MD in AAB30100. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform RII-2 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 1A12D58550921F5E

FASTA59267,122
        10         20         30         40         50         60 
MGRGLLRGLW PLHIVLWTRI ASTIPPHVPK SDVEMEAQKD ASIHLSCNRT IHPLKHFNSD 

        70         80         90        100        110        120 
VMASDNGGAV KLPQLCKFCD VRLSTCDNQK SCMSNCSITA ICEKPHEVCV AVWRKNDKNI 

       130        140        150        160        170        180 
TLETVCHDPK LTYHGFTLED AASPKCVMKE KKRAGETFFM CACNMEECND YIIFSEEYTT 

       190        200        210        220        230        240 
SSPDLLLVII QVTGVSLLPP LGIAIAVIII FYCYRVHRQQ KLSPSWESSK PRKLMDFSDN 

       250        260        270        280        290        300 
CAIILEDDRS DISSTCANNI NHNTELLPIE LDTLVGKGRF AEVYKAKLKQ NTSEQFETVA 

       310        320        330        340        350        360 
VKIFPYEEYS SWKTEKDIFS DINLKHENIL QFLTAEERKT ELGKQYWLIT AFHAKGNLQE 

       370        380        390        400        410        420 
YLTRHVISWE DLRKLGSSLA RGIAHLHSDH TPCGRPKMPI VHRDLKSSNI LVKNDLTCCL 

       430        440        450        460        470        480 
CDFGLSLRLD PTLSVDDLAN SGQVGTARYM APEVLESRMN LENVESFKQT DVYSMALVLW 

       490        500        510        520        530        540 
EMTSRCNAVG EVKDYEPPFG SKVREHPCVE SMKDSVLRDR GRPEIPSFWL NHQGIQIVCE 

       550        560        570        580        590 
TLTECWDHDP EARLTAQCVA ERFSELEHPE RLSGRSCSQE KIPEDGSLNT TK 

« Hide

Isoform RII-1 [UniParc].

Checksum: 4C66067ADA84F986
Show »

56764,219

References

« Hide 'large scale' references
[1]"Cloning of an isoform of mouse TGF-beta type II receptor gene."
Suzuki A., Shioda N., Maeda T., Tada M., Ueno N.
FEBS Lett. 355:19-22(1994) [PubMed: 7957954] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
Tissue: Brain.
[2]"The murine type II TGF-beta receptor has a coincident embryonic expression and binding preference for TGF-beta 1."
Lawler S., Candia A.F., Ebner R., Shum L., Lopez A.R., Moses H.L., Wright C.V., Derynck R.
Development 120:165-175(1994) [PubMed: 8119124] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM RII-2).
Tissue: Olfactory epithelium.
+Additional computationally mapped references.

Cross-references

Sequence databases

D32072 mRNA. Translation: BAA06840.1.
S69114 mRNA. Translation: AAB30100.2.
BC052629 mRNA. Translation: AAH52629.1.
PIRS51371.
UniGeneMm.172346

3D structure databases

HSSPHSSP built from PDB template 1KTZ based on UniProtKB P37173.
SMRQ62312. Positions 64-184.
ModBaseSearch...

PTM databases

PhosphoSiteQ62312.

Genome annotation databases

EnsemblENSMUSG00000032440. Mus musculus. [Contig view]

Organism-specific databases

MGIMGI:98729. Tgfbr2.

Phylogenomic databases

HOGENOMQ62312.
HOVERGENQ62312.

Gene expression databases

ArrayExpressQ62312.
CleanExMM_TGFBR2.
GermOnlineENSMUSG00000032440. Mus musculus.

Family and domain databases

InterProIPR000333. Activin_II_recpt.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_bd_CS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR015769. TGF-beta_II_C.
IPR015013. TGF_beta_receptor2.
IPR017194. TGFRII.
[Graphical view]
PANTHERPTHR23255:SF11. TGF-beta_II_C. 1 hit.
PfamPF08917. ecTbetaR2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF037393. TGFRII. 1 hit.
PRINTSPR00653. ACTIVIN2R.
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

Entry information

Entry nameTGFR2_MOUSE
AccessionPrimary (citable) accession number: Q62312
Secondary accession number(s): Q63947
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents