Reviewed, UniProtKB/Swiss-Prot Q62312 (TGFR2_MOUSE)

Names and origin

Protein names

Recommended name:
    TGF-beta receptor type-2
    EC=2.7.11.30
Alternative name(s):
    Transforming growth factor-beta receptor type II
      Short name=TGF-beta receptor type II
    TGF-beta type II receptor
    TbetaR-II
    TGFR-2

Gene names

Name:

Tgfbr2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta.
Catalytic activity	ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.
Cofactor	Magnesium or manganese By similarity.
Subunit structure	Binds to DAXX. Interacts with TCTEX1D4 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Widely expressed in adult. Expressed primarily in mesenchyme and epidermis of the midgestational fetus.
Post-translational modification	Phosphorylated on a Ser/Thr residue in the cytoplasmic domain By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	activation of protein kinase activity Inferred from direct assay. Source: MGI brain development Inferred from mutant phenotype. Source: UniProtKB embryonic cranial skeleton morphogenesis Inferred from mutant phenotype. Source: MGI embryonic hemopoiesis Inferred from mutant phenotype. Source: UniProtKB heart development Inferred from mutant phenotype. Source: MGI myeloid dendritic cell differentiation Inferred from mutant phenotype. Source: MGI palate development Inferred from mutant phenotype. Source: UniProtKB patterning of blood vessels Inferred from mutant phenotype. Source: MGI positive regulation of B cell tolerance induction Inferred from mutant phenotype. Source: MGI positive regulation of NK T cell differentiation Inferred from mutant phenotype. Source: MGI positive regulation of T cell tolerance induction Inferred from mutant phenotype. Source: MGI positive regulation of mesenchymal cell proliferation Inferred from mutant phenotype. Source: UniProtKB positive regulation of tolerance induction to self antigen Inferred from mutant phenotype. Source: MGI protein amino acid phosphorylation Traceable author statement. Source: MGI transmembrane receptor protein serine/threonine kinase signaling pathway Inferred from electronic annotation. Source: InterPro vasculogenesis Inferred from mutant phenotype. Source: UniProtKB
Cellular component	external side of plasma membrane Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: UniProtKB receptor activity Inferred from electronic annotation. Source: UniProtKB-KW receptor signaling protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform RII-2 (identifier: Q62312-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform RII-1 (identifier: Q62312-2)

The sequence of this isoform differs from the canonical sequence as follows:
     32-56: Missing.
     57-57: F → V

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 592	569	TGF-beta receptor type-2		PRO_0000024427
Regions
	Topological domain	24 – 184	161	Extracellular Potential
	Transmembrane	185 – 214	30	Potential
	Topological domain	215 – 592	378	Cytoplasmic Potential
	Domain	269 – 569	301	Protein kinase
	Nucleotide binding	275 – 283	9	ATP By similarity
Sites
	Active site	404	1	Proton acceptor By similarity
	Binding site	302	1	ATP By similarity
Amino acid modifications
	Modified residue	573	1	Phosphoserine By similarity
	Glycosylation	48	1	N-linked (GlcNAc...) Potential
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential
	Disulfide bond	76 ↔ 109		By similarity
	Disulfide bond	79 ↔ 96		By similarity
	Disulfide bond	86 ↔ 92		By similarity
	Disulfide bond	102 ↔ 126		By similarity
	Disulfide bond	146 ↔ 161		By similarity
	Disulfide bond	163 ↔ 168		By similarity
Natural variations
	Alternative sequence	32 – 56	25	Missing in isoform RII-1.		VSP_004955
	Alternative sequence	57	1	F → V in isoform RII-1.		VSP_004956
Experimental info
	Sequence conflict	8	1	G → A in AAB30100. Ref.2
	Sequence conflict	117	1	D → N in AAB30100. Ref.2
	Sequence conflict	354	1	A → R in AAB30100. Ref.2
	Sequence conflict	440 – 443	4	NSGQ → KQRE in AAB30100. Ref.2
	Sequence conflict	486	1	C → W in AAB30100. Ref.2
	Sequence conflict	506	1	H → P in AAB30100. Ref.2
	Sequence conflict	569 – 570	2	PE → MD in AAB30100. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform RII-2 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1A12D58550921F5E	FASTA	592	67,122
	10 20 30 40 50 60 MGRGLLRGLW PLHIVLWTRI ASTIPPHVPK SDVEMEAQKD ASIHLSCNRT IHPLKHFNSD 70 80 90 100 110 120 VMASDNGGAV KLPQLCKFCD VRLSTCDNQK SCMSNCSITA ICEKPHEVCV AVWRKNDKNI 130 140 150 160 170 180 TLETVCHDPK LTYHGFTLED AASPKCVMKE KKRAGETFFM CACNMEECND YIIFSEEYTT 190 200 210 220 230 240 SSPDLLLVII QVTGVSLLPP LGIAIAVIII FYCYRVHRQQ KLSPSWESSK PRKLMDFSDN 250 260 270 280 290 300 CAIILEDDRS DISSTCANNI NHNTELLPIE LDTLVGKGRF AEVYKAKLKQ NTSEQFETVA 310 320 330 340 350 360 VKIFPYEEYS SWKTEKDIFS DINLKHENIL QFLTAEERKT ELGKQYWLIT AFHAKGNLQE 370 380 390 400 410 420 YLTRHVISWE DLRKLGSSLA RGIAHLHSDH TPCGRPKMPI VHRDLKSSNI LVKNDLTCCL 430 440 450 460 470 480 CDFGLSLRLD PTLSVDDLAN SGQVGTARYM APEVLESRMN LENVESFKQT DVYSMALVLW 490 500 510 520 530 540 EMTSRCNAVG EVKDYEPPFG SKVREHPCVE SMKDSVLRDR GRPEIPSFWL NHQGIQIVCE 550 560 570 580 590 TLTECWDHDP EARLTAQCVA ERFSELEHPE RLSGRSCSQE KIPEDGSLNT TK
	Isoform RII-1 [UniParc]. Checksum: 4C66067ADA84F986		567	64,219
	10 20 30 40 50 60 MGRGLLRGLW PLHIVLWTRI ASTIPPHVPK SVNSDVMASD NGGAVKLPQL CKFCDVRLST 70 80 90 100 110 120 CDNQKSCMSN CSITAICEKP HEVCVAVWRK NDKNITLETV CHDPKLTYHG FTLEDAASPK 130 140 150 160 170 180 CVMKEKKRAG ETFFMCACNM EECNDYIIFS EEYTTSSPDL LLVIIQVTGV SLLPPLGIAI 190 200 210 220 230 240 AVIIIFYCYR VHRQQKLSPS WESSKPRKLM DFSDNCAIIL EDDRSDISST CANNINHNTE 250 260 270 280 290 300 LLPIELDTLV GKGRFAEVYK AKLKQNTSEQ FETVAVKIFP YEEYSSWKTE KDIFSDINLK 310 320 330 340 350 360 HENILQFLTA EERKTELGKQ YWLITAFHAK GNLQEYLTRH VISWEDLRKL GSSLARGIAH 370 380 390 400 410 420 LHSDHTPCGR PKMPIVHRDL KSSNILVKND LTCCLCDFGL SLRLDPTLSV DDLANSGQVG 430 440 450 460 470 480 TARYMAPEVL ESRMNLENVE SFKQTDVYSM ALVLWEMTSR CNAVGEVKDY EPPFGSKVRE 490 500 510 520 530 540 HPCVESMKDS VLRDRGRPEI PSFWLNHQGI QIVCETLTEC WDHDPEARLT AQCVAERFSE 550 560 LEHPERLSGR SCSQEKIPED GSLNTTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of an isoform of mouse TGF-beta type II receptor gene." Suzuki A., Shioda N., Maeda T., Tada M., Ueno N. FEBS Lett. 355:19-22(1994) [PubMed: 7957954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING. Tissue: Brain.
[2]	"The murine type II TGF-beta receptor has a coincident embryonic expression and binding preference for TGF-beta 1." Lawler S., Candia A.F., Ebner R., Shum L., Lopez A.R., Moses H.L., Wright C.V., Derynck R. Development 120:165-175(1994) [PubMed: 8119124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM RII-2). Tissue: Olfactory epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D32072 mRNA. Translation: BAA06840.1. S69114 mRNA. Translation: AAB30100.2. BC052629 mRNA. Translation: AAH52629.1.
PIR	S51371.
UniGene	Mm.172346
3D structure databases
HSSP	HSSP built from PDB template 1KTZ based on UniProtKB P37173.
SMR	Q62312. Positions 64-184.
ModBase	Search...
PTM databases
PhosphoSite	Q62312.
Genome annotation databases
Ensembl	ENSMUSG00000032440. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:98729. Tgfbr2.
Phylogenomic databases
HOGENOM	Q62312.
HOVERGEN	Q62312.
Gene expression databases
ArrayExpress	Q62312.
CleanEx	MM_TGFBR2.
GermOnline	ENSMUSG00000032440. Mus musculus.
Family and domain databases
InterPro	IPR000333. Activin_II_recpt. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR015769. TGF-beta_II_C. IPR015013. TGF_beta_receptor2. IPR017194. TGFRII. [Graphical view]
PANTHER	PTHR23255:SF11. TGF-beta_II_C. 1 hit.
Pfam	PF08917. ecTbetaR2. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF037393. TGFRII. 1 hit.
PRINTS	PR00653. ACTIVIN2R.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

TGFR2_MOUSE

Accession

Primary (citable) accession number: Q62312
Secondary accession number(s): Q63947

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)