Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q62296

- TEAD4_MOUSE

UniProt

Q62296 - TEAD4_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Transcriptional enhancer factor TEF-3

Gene

Tead4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif (By similarity). Might play a role in the embryonic development of skeletal muscle.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi31 – 9868TEAPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. blastocyst formation Source: MGI
  2. cell fate specification Source: MGI
  3. embryo implantation Source: MGI
  4. hippo signaling Source: InterPro
  5. in utero embryonic development Source: MGI
  6. positive regulation of stem cell maintenance Source: MGI
  7. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  8. regulation of transcription from RNA polymerase II promoter Source: MGI
  9. transcription, DNA-templated Source: UniProtKB-KW
  10. trophectodermal cell fate commitment Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_196516. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
REACT_198602. PPARA activates gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional enhancer factor TEF-3
Alternative name(s):
ETF-related factor 2
Short name:
ETFR-2
TEA domain family member 4
Short name:
TEAD-4
TEF-1-related factor 1
TEF-1-related factor FR-19
Short name:
RTEF-1
Gene namesi
Name:Tead4
Synonyms:Tcf13r1, Tef3, Tefr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:106907. Tead4.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Transcriptional enhancer factor TEF-3PRO_0000205938Add
BLAST

Proteomic databases

PRIDEiQ62296.

PTM databases

PhosphoSiteiQ62296.

Expressioni

Tissue specificityi

Preferentially expressed in lung and in skeletal muscle.

Inductioni

By FGF-1, FGF-2, calf serum, platelet-derived growth factor-BB, and phorbol 12-myristate 13-acetate.

Gene expression databases

BgeeiQ62296.
CleanExiMM_TEAD4.
ExpressionAtlasiQ62296. baseline.
GenevestigatoriQ62296.

Interactioni

Subunit structurei

Interacts with WWTR1/TAZ (By similarity). Interacts with YAP1.By similarity1 Publication

Protein-protein interaction databases

BioGridi204101. 3 interactions.
DIPiDIP-59847N.

Structurei

Secondary structure

1
427
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi210 – 2134
Beta strandi218 – 2269
Beta strandi239 – 2446
Beta strandi250 – 2523
Beta strandi257 – 2593
Helixi260 – 2623
Helixi264 – 2663
Turni270 – 2723
Helixi274 – 2807
Beta strandi285 – 2939
Helixi299 – 3013
Beta strandi307 – 31711
Beta strandi321 – 3299
Beta strandi332 – 34110
Beta strandi344 – 3463
Beta strandi349 – 35810
Helixi361 – 37010
Beta strandi372 – 3743
Helixi376 – 3838
Beta strandi386 – 39712
Beta strandi400 – 41011
Beta strandi412 – 4154
Beta strandi419 – 4257

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JUAX-ray3.00A/C/E/G210-427[»]
4EAZX-ray2.80A/B210-427[»]
4LN0X-ray2.90A/B209-427[»]
ProteinModelPortaliQ62296.
SMRiQ62296. Positions 28-106, 210-426.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62296.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi78 – 9417Nuclear localization signalSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 TEA DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG246992.
GeneTreeiENSGT00390000008670.
HOGENOMiHOG000253933.
HOVERGENiHBG056905.
InParanoidiQ62296.
KOiK09448.
OrthoDBiEOG7288RP.
PhylomeDBiQ62296.
TreeFamiTF313443.

Family and domain databases

InterProiIPR000818. TEA/ATTS.
IPR016361. TEF.
IPR027255. TEF-3.
[Graphical view]
PANTHERiPTHR11834. PTHR11834. 1 hit.
PfamiPF01285. TEA. 1 hit.
[Graphical view]
PIRSFiPIRSF002603. TEF. 1 hit.
PIRSF500722. TEF-3. 1 hit.
PRINTSiPR00065. TEADOMAIN.
SMARTiSM00426. TEA. 1 hit.
[Graphical view]
PROSITEiPS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: Q62296-1) [UniParc]FASTAAdd to Basket

Also known as: TEFR1A, ETFR-2A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSNEWSSPD SPEGSSISGG SQALDKPIDN DAEGVWSPEI ERSFQEALAI
60 70 80 90 100
YPPCGRRKII LTEEGKMYGR NELIARHIKL RTGKTRTRKQ VSSHIQVLAR
110 120 130 140 150
RKAREIQAKL KDQAAKNKAL QSMAAMSSAQ IVSATAFHSK MALARGPGYP
160 170 180 190 200
AISGFWQGAL PGQPGTSHDV KPFSQNTYPV QPPLPLPGFE SPAGPTPSPS
210 220 230 240 250
APLAPPWQGR SIASSKLWML EFSAFLERQQ DPDTYNKHLF VHISQSSPSY
260 270 280 290 300
SDPYLETVDI RQIYDKFPEK KGGLKELFER GPSNAFFLVK FWADLNTNID
310 320 330 340 350
DEGSAFYGVS SQYESPENMI ITCSTKVCSF GKQVVEKVET EYARYENGHY
360 370 380 390 400
LYRIHRSPLC EYMINFIHKL KHLPEKYMMN SVLENFTILQ VVTNRDTQET
410 420
LLCIAYVFEV SASEHGAQHH IYRLVKE
Length:427
Mass (Da):48,028
Last modified:November 1, 1997 - v2
Checksum:i8A0E9749840D88EC
GO
Isoform Short (identifier: Q62296-2) [UniParc]FASTAAdd to Basket

Also known as: TEFR1B, ETFR-2B

The sequence of this isoform differs from the canonical sequence as follows:
     112-154: Missing.

Show »
Length:384
Mass (Da):43,710
Checksum:i07B8CFF2E0E3798B
GO

Sequence cautioni

The sequence AAB12488.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon.
The sequence AAB12488.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti142 – 1421A → L in AAB12488. (PubMed:8631987)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei112 – 15443Missing in isoform Short. 4 PublicationsVSP_006390Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94441 mRNA. Translation: CAA64215.2.
D87966 mRNA. Translation: BAA13519.1.
D87965 mRNA. Translation: BAA13518.1.
L26343 mRNA. Translation: AAB12488.1. Sequence problems.
L26344 mRNA. Translation: AAC37680.1.
BC130257 mRNA. Translation: AAI30258.1.
U51743 mRNA. Translation: AAC52646.1.
U51745 mRNA. Translation: AAC52647.1.
CCDSiCCDS39645.1. [Q62296-2]
CCDS39646.1. [Q62296-1]
PIRiJC5254.
JC5255.
RefSeqiNP_001074448.2. NM_001080979.1. [Q62296-2]
NP_035697.3. NM_011567.2. [Q62296-1]
UniGeneiMm.14774.

Genome annotation databases

EnsembliENSMUST00000006311; ENSMUSP00000006311; ENSMUSG00000030353.
ENSMUST00000112157; ENSMUSP00000107784; ENSMUSG00000030353.
ENSMUST00000130454; ENSMUSP00000118083; ENSMUSG00000030353.
GeneIDi21679.
KEGGimmu:21679.
UCSCiuc009ede.1. mouse. [Q62296-1]
uc009edf.1. mouse. [Q62296-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X94441 mRNA. Translation: CAA64215.2 .
D87966 mRNA. Translation: BAA13519.1 .
D87965 mRNA. Translation: BAA13518.1 .
L26343 mRNA. Translation: AAB12488.1 . Sequence problems.
L26344 mRNA. Translation: AAC37680.1 .
BC130257 mRNA. Translation: AAI30258.1 .
U51743 mRNA. Translation: AAC52646.1 .
U51745 mRNA. Translation: AAC52647.1 .
CCDSi CCDS39645.1. [Q62296-2 ]
CCDS39646.1. [Q62296-1 ]
PIRi JC5254.
JC5255.
RefSeqi NP_001074448.2. NM_001080979.1. [Q62296-2 ]
NP_035697.3. NM_011567.2. [Q62296-1 ]
UniGenei Mm.14774.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3JUA X-ray 3.00 A/C/E/G 210-427 [» ]
4EAZ X-ray 2.80 A/B 210-427 [» ]
4LN0 X-ray 2.90 A/B 209-427 [» ]
ProteinModelPortali Q62296.
SMRi Q62296. Positions 28-106, 210-426.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204101. 3 interactions.
DIPi DIP-59847N.

PTM databases

PhosphoSitei Q62296.

Proteomic databases

PRIDEi Q62296.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000006311 ; ENSMUSP00000006311 ; ENSMUSG00000030353 .
ENSMUST00000112157 ; ENSMUSP00000107784 ; ENSMUSG00000030353 .
ENSMUST00000130454 ; ENSMUSP00000118083 ; ENSMUSG00000030353 .
GeneIDi 21679.
KEGGi mmu:21679.
UCSCi uc009ede.1. mouse. [Q62296-1 ]
uc009edf.1. mouse. [Q62296-2 ]

Organism-specific databases

CTDi 7004.
MGIi MGI:106907. Tead4.

Phylogenomic databases

eggNOGi NOG246992.
GeneTreei ENSGT00390000008670.
HOGENOMi HOG000253933.
HOVERGENi HBG056905.
InParanoidi Q62296.
KOi K09448.
OrthoDBi EOG7288RP.
PhylomeDBi Q62296.
TreeFami TF313443.

Enzyme and pathway databases

Reactomei REACT_196516. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
REACT_198602. PPARA activates gene expression.

Miscellaneous databases

EvolutionaryTracei Q62296.
NextBioi 300982.
PROi Q62296.
SOURCEi Search...

Gene expression databases

Bgeei Q62296.
CleanExi MM_TEAD4.
ExpressionAtlasi Q62296. baseline.
Genevestigatori Q62296.

Family and domain databases

InterProi IPR000818. TEA/ATTS.
IPR016361. TEF.
IPR027255. TEF-3.
[Graphical view ]
PANTHERi PTHR11834. PTHR11834. 1 hit.
Pfami PF01285. TEA. 1 hit.
[Graphical view ]
PIRSFi PIRSF002603. TEF. 1 hit.
PIRSF500722. TEF-3. 1 hit.
PRINTSi PR00065. TEADOMAIN.
SMARTi SM00426. TEA. 1 hit.
[Graphical view ]
PROSITEi PS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain."
    Jacquemin P., Hwang J.-J., Martial J.A., Dolle P., Davidson I.
    J. Biol. Chem. 271:21775-21785(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A novel family of TEA domain-containing transcription factors with distinct spatiotemporal expression patterns."
    Yasunami M., Suzuki K., Ohkubo H.
    Biochem. Biophys. Res. Commun. 228:365-370(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
  3. "cDNA cloning and characterization of murine transcriptional enhancer factor-1-related protein 1, a transcription factor that binds to the M-CAT motif."
    Yockey C.E., Smith G., Izumo S., Shimizu N.
    J. Biol. Chem. 271:3727-3736(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Strain: C3H.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
  5. "Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation."
    Hsu D.K.W., Guo Y., Alberts G.F., Copeland N.G., Gilbert D.J., Jenkins N.A., Peifley K.A., Winkles J.A.
    J. Biol. Chem. 271:13786-13795(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-427 (ISOFORMS LONG AND SHORT).
    Strain: BALB/c.
  6. "Structural basis of YAP recognition by TEAD4 in the hippo pathway."
    Chen L., Chan S.W., Zhang X., Walsh M., Lim C.J., Hong W., Song H.
    Genes Dev. 24:290-300(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 210-427 IN COMPLEX WITH YAP1.

Entry informationi

Entry nameiTEAD4_MOUSE
AccessioniPrimary (citable) accession number: Q62296
Secondary accession number(s): A2BDD5
, P70282, Q61174, Q61175, Q62298
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3