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Q62296

- TEAD4_MOUSE

UniProt

Q62296 - TEAD4_MOUSE

Protein

Transcriptional enhancer factor TEF-3

Gene

Tead4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif By similarity. Might play a role in the embryonic development of skeletal muscle.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi31 – 9868TEAPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. blastocyst formation Source: MGI
    2. cell fate specification Source: MGI
    3. embryo implantation Source: MGI
    4. hippo signaling Source: InterPro
    5. in utero embryonic development Source: MGI
    6. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    7. regulation of transcription from RNA polymerase II promoter Source: MGI
    8. transcription, DNA-templated Source: UniProtKB-KW
    9. trophectodermal cell fate commitment Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_196516. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
    REACT_198602. PPARA activates gene expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional enhancer factor TEF-3
    Alternative name(s):
    ETF-related factor 2
    Short name:
    ETFR-2
    TEA domain family member 4
    Short name:
    TEAD-4
    TEF-1-related factor 1
    TEF-1-related factor FR-19
    Short name:
    RTEF-1
    Gene namesi
    Name:Tead4
    Synonyms:Tcf13r1, Tef3, Tefr1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:106907. Tead4.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427Transcriptional enhancer factor TEF-3PRO_0000205938Add
    BLAST

    Proteomic databases

    PRIDEiQ62296.

    PTM databases

    PhosphoSiteiQ62296.

    Expressioni

    Tissue specificityi

    Preferentially expressed in lung and in skeletal muscle.

    Inductioni

    By FGF-1, FGF-2, calf serum, platelet-derived growth factor-BB, and phorbol 12-myristate 13-acetate.

    Gene expression databases

    ArrayExpressiQ62296.
    BgeeiQ62296.
    CleanExiMM_TEAD4.
    GenevestigatoriQ62296.

    Interactioni

    Subunit structurei

    Interacts with WWTR1/TAZ By similarity. Interacts with YAP1.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi204101. 3 interactions.
    DIPiDIP-59847N.

    Structurei

    Secondary structure

    1
    427
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi210 – 2134
    Beta strandi218 – 2269
    Beta strandi239 – 2446
    Beta strandi250 – 2523
    Beta strandi257 – 2593
    Helixi260 – 2623
    Helixi264 – 2663
    Turni270 – 2723
    Helixi274 – 2807
    Beta strandi285 – 2939
    Helixi299 – 3013
    Beta strandi307 – 31711
    Beta strandi321 – 3299
    Beta strandi332 – 34110
    Beta strandi344 – 3463
    Beta strandi349 – 35810
    Helixi361 – 37010
    Beta strandi372 – 3743
    Helixi376 – 3838
    Beta strandi386 – 39712
    Beta strandi400 – 41011
    Beta strandi412 – 4154
    Beta strandi419 – 4257

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3JUAX-ray3.00A/C/E/G210-427[»]
    4EAZX-ray2.80A/B210-427[»]
    4LN0X-ray2.90A/B209-427[»]
    ProteinModelPortaliQ62296.
    SMRiQ62296. Positions 28-106, 210-426.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ62296.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi78 – 9417Nuclear localization signalSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 TEA DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG246992.
    GeneTreeiENSGT00390000008670.
    HOGENOMiHOG000253933.
    HOVERGENiHBG056905.
    InParanoidiQ62296.
    KOiK09448.
    OrthoDBiEOG7288RP.
    PhylomeDBiQ62296.
    TreeFamiTF313443.

    Family and domain databases

    InterProiIPR000818. TEA/ATTS.
    IPR016361. TEF.
    IPR027255. TEF-3.
    [Graphical view]
    PANTHERiPTHR11834. PTHR11834. 1 hit.
    PfamiPF01285. TEA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002603. TEF. 1 hit.
    PIRSF500722. TEF-3. 1 hit.
    PRINTSiPR00065. TEADOMAIN.
    SMARTiSM00426. TEA. 1 hit.
    [Graphical view]
    PROSITEiPS00554. TEA_1. 1 hit.
    PS51088. TEA_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q62296-1) [UniParc]FASTAAdd to Basket

    Also known as: TEFR1A, ETFR-2A

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTSNEWSSPD SPEGSSISGG SQALDKPIDN DAEGVWSPEI ERSFQEALAI    50
    YPPCGRRKII LTEEGKMYGR NELIARHIKL RTGKTRTRKQ VSSHIQVLAR 100
    RKAREIQAKL KDQAAKNKAL QSMAAMSSAQ IVSATAFHSK MALARGPGYP 150
    AISGFWQGAL PGQPGTSHDV KPFSQNTYPV QPPLPLPGFE SPAGPTPSPS 200
    APLAPPWQGR SIASSKLWML EFSAFLERQQ DPDTYNKHLF VHISQSSPSY 250
    SDPYLETVDI RQIYDKFPEK KGGLKELFER GPSNAFFLVK FWADLNTNID 300
    DEGSAFYGVS SQYESPENMI ITCSTKVCSF GKQVVEKVET EYARYENGHY 350
    LYRIHRSPLC EYMINFIHKL KHLPEKYMMN SVLENFTILQ VVTNRDTQET 400
    LLCIAYVFEV SASEHGAQHH IYRLVKE 427
    Length:427
    Mass (Da):48,028
    Last modified:November 1, 1997 - v2
    Checksum:i8A0E9749840D88EC
    GO
    Isoform Short (identifier: Q62296-2) [UniParc]FASTAAdd to Basket

    Also known as: TEFR1B, ETFR-2B

    The sequence of this isoform differs from the canonical sequence as follows:
         112-154: Missing.

    Show »
    Length:384
    Mass (Da):43,710
    Checksum:i07B8CFF2E0E3798B
    GO

    Sequence cautioni

    The sequence AAB12488.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon.
    The sequence AAB12488.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti142 – 1421A → L in AAB12488. (PubMed:8631987)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei112 – 15443Missing in isoform Short. 4 PublicationsVSP_006390Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94441 mRNA. Translation: CAA64215.2.
    D87966 mRNA. Translation: BAA13519.1.
    D87965 mRNA. Translation: BAA13518.1.
    L26343 mRNA. Translation: AAB12488.1. Sequence problems.
    L26344 mRNA. Translation: AAC37680.1.
    BC130257 mRNA. Translation: AAI30258.1.
    U51743 mRNA. Translation: AAC52646.1.
    U51745 mRNA. Translation: AAC52647.1.
    CCDSiCCDS39645.1. [Q62296-2]
    CCDS39646.1. [Q62296-1]
    PIRiJC5254.
    JC5255.
    RefSeqiNP_001074448.2. NM_001080979.1. [Q62296-2]
    NP_035697.3. NM_011567.2. [Q62296-1]
    UniGeneiMm.14774.

    Genome annotation databases

    EnsembliENSMUST00000006311; ENSMUSP00000006311; ENSMUSG00000030353.
    ENSMUST00000112157; ENSMUSP00000107784; ENSMUSG00000030353.
    ENSMUST00000130454; ENSMUSP00000118083; ENSMUSG00000030353.
    GeneIDi21679.
    KEGGimmu:21679.
    UCSCiuc009ede.1. mouse. [Q62296-1]
    uc009edf.1. mouse. [Q62296-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94441 mRNA. Translation: CAA64215.2 .
    D87966 mRNA. Translation: BAA13519.1 .
    D87965 mRNA. Translation: BAA13518.1 .
    L26343 mRNA. Translation: AAB12488.1 . Sequence problems.
    L26344 mRNA. Translation: AAC37680.1 .
    BC130257 mRNA. Translation: AAI30258.1 .
    U51743 mRNA. Translation: AAC52646.1 .
    U51745 mRNA. Translation: AAC52647.1 .
    CCDSi CCDS39645.1. [Q62296-2 ]
    CCDS39646.1. [Q62296-1 ]
    PIRi JC5254.
    JC5255.
    RefSeqi NP_001074448.2. NM_001080979.1. [Q62296-2 ]
    NP_035697.3. NM_011567.2. [Q62296-1 ]
    UniGenei Mm.14774.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3JUA X-ray 3.00 A/C/E/G 210-427 [» ]
    4EAZ X-ray 2.80 A/B 210-427 [» ]
    4LN0 X-ray 2.90 A/B 209-427 [» ]
    ProteinModelPortali Q62296.
    SMRi Q62296. Positions 28-106, 210-426.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204101. 3 interactions.
    DIPi DIP-59847N.

    PTM databases

    PhosphoSitei Q62296.

    Proteomic databases

    PRIDEi Q62296.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006311 ; ENSMUSP00000006311 ; ENSMUSG00000030353 .
    ENSMUST00000112157 ; ENSMUSP00000107784 ; ENSMUSG00000030353 .
    ENSMUST00000130454 ; ENSMUSP00000118083 ; ENSMUSG00000030353 .
    GeneIDi 21679.
    KEGGi mmu:21679.
    UCSCi uc009ede.1. mouse. [Q62296-1 ]
    uc009edf.1. mouse. [Q62296-2 ]

    Organism-specific databases

    CTDi 7004.
    MGIi MGI:106907. Tead4.

    Phylogenomic databases

    eggNOGi NOG246992.
    GeneTreei ENSGT00390000008670.
    HOGENOMi HOG000253933.
    HOVERGENi HBG056905.
    InParanoidi Q62296.
    KOi K09448.
    OrthoDBi EOG7288RP.
    PhylomeDBi Q62296.
    TreeFami TF313443.

    Enzyme and pathway databases

    Reactomei REACT_196516. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
    REACT_198602. PPARA activates gene expression.

    Miscellaneous databases

    EvolutionaryTracei Q62296.
    NextBioi 300982.
    PROi Q62296.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q62296.
    Bgeei Q62296.
    CleanExi MM_TEAD4.
    Genevestigatori Q62296.

    Family and domain databases

    InterProi IPR000818. TEA/ATTS.
    IPR016361. TEF.
    IPR027255. TEF-3.
    [Graphical view ]
    PANTHERi PTHR11834. PTHR11834. 1 hit.
    Pfami PF01285. TEA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002603. TEF. 1 hit.
    PIRSF500722. TEF-3. 1 hit.
    PRINTSi PR00065. TEADOMAIN.
    SMARTi SM00426. TEA. 1 hit.
    [Graphical view ]
    PROSITEi PS00554. TEA_1. 1 hit.
    PS51088. TEA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain."
      Jacquemin P., Hwang J.-J., Martial J.A., Dolle P., Davidson I.
      J. Biol. Chem. 271:21775-21785(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "A novel family of TEA domain-containing transcription factors with distinct spatiotemporal expression patterns."
      Yasunami M., Suzuki K., Ohkubo H.
      Biochem. Biophys. Res. Commun. 228:365-370(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    3. "cDNA cloning and characterization of murine transcriptional enhancer factor-1-related protein 1, a transcription factor that binds to the M-CAT motif."
      Yockey C.E., Smith G., Izumo S., Shimizu N.
      J. Biol. Chem. 271:3727-3736(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
      Strain: C3H.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
    5. "Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation."
      Hsu D.K.W., Guo Y., Alberts G.F., Copeland N.G., Gilbert D.J., Jenkins N.A., Peifley K.A., Winkles J.A.
      J. Biol. Chem. 271:13786-13795(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-427 (ISOFORMS LONG AND SHORT).
      Strain: BALB/c.
    6. "Structural basis of YAP recognition by TEAD4 in the hippo pathway."
      Chen L., Chan S.W., Zhang X., Walsh M., Lim C.J., Hong W., Song H.
      Genes Dev. 24:290-300(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 210-427 IN COMPLEX WITH YAP1.

    Entry informationi

    Entry nameiTEAD4_MOUSE
    AccessioniPrimary (citable) accession number: Q62296
    Secondary accession number(s): A2BDD5
    , P70282, Q61174, Q61175, Q62298
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3