Reviewed, UniProtKB/Swiss-Prot Q62296 (TEAD4_MOUSE)

Names and origin

Protein names

Recommended name:
    Transcriptional enhancer factor TEF-3
Alternative name(s):
    TEA domain family member 4
      Short name=TEAD-4
    ETF-related factor 2
      Short name=ETFR-2
    TEF-1-related factor 1
    TEF-1-related factor FR-19
      Short name=RTEF-1

Gene names

Name:	Tead4
Synonyms:	Tcf13r1, Tef3, Tefr1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Binds specifically and non-cooperatively to the SPH and GT-IIC "enhansons" (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif. Might play a role in the embryonic development of skeletal muscle.
Subcellular location	Nucleus.
Tissue specificity	Preferentially expressed in lung and in skeletal muscle.
Induction	By FGF-1, FGF-2, calf serum, platelet-derived growth factor-BB, and phorbol 12-myristate 13-acetate.
Sequence similarities	Contains 1 TEA DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
Gene Ontology (GO)
Biological process	cell fate specification Inferred from mutant phenotype. Source: MGI embryo implantation Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW trophectodermal cell fate commitment Inferred from mutant phenotype. Source: MGI
Cellular component	transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	transcription activator activity Inferred from genetic interaction. Source: MGI transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q62296-1)

Also known as: TEFR1A; ETFR-2A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q62296-2)

Also known as: TEFR1B; ETFR-2B;

The sequence of this isoform differs from the canonical sequence as follows:
     112-154: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Transcriptional enhancer factor TEF-3		PRO_0000205938
Regions
	DNA binding	31 – 98	68	TEA
	Motif	78 – 94	17	Nuclear localization signal Potential
Natural variations
	Alternative sequence	112 – 154	43	Missing in isoform Short.		VSP_006390
Experimental info
	Sequence conflict	142	1	A → L in AAB12488. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long (TEFR1A) (ETFR-2A) [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 8A0E9749840D88EC	FASTA	427	48,028
	10 20 30 40 50 60 MTSNEWSSPD SPEGSSISGG SQALDKPIDN DAEGVWSPEI ERSFQEALAI YPPCGRRKII 70 80 90 100 110 120 LTEEGKMYGR NELIARHIKL RTGKTRTRKQ VSSHIQVLAR RKAREIQAKL KDQAAKNKAL 130 140 150 160 170 180 QSMAAMSSAQ IVSATAFHSK MALARGPGYP AISGFWQGAL PGQPGTSHDV KPFSQNTYPV 190 200 210 220 230 240 QPPLPLPGFE SPAGPTPSPS APLAPPWQGR SIASSKLWML EFSAFLERQQ DPDTYNKHLF 250 260 270 280 290 300 VHISQSSPSY SDPYLETVDI RQIYDKFPEK KGGLKELFER GPSNAFFLVK FWADLNTNID 310 320 330 340 350 360 DEGSAFYGVS SQYESPENMI ITCSTKVCSF GKQVVEKVET EYARYENGHY LYRIHRSPLC 370 380 390 400 410 420 EYMINFIHKL KHLPEKYMMN SVLENFTILQ VVTNRDTQET LLCIAYVFEV SASEHGAQHH IYRLVKE
	Isoform Short (TEFR1B) (ETFR-2B). Checksum: 07B8CFF2E0E3798B	FASTA	384	43,710
	10 20 30 40 50 60 MTSNEWSSPD SPEGSSISGG SQALDKPIDN DAEGVWSPEI ERSFQEALAI YPPCGRRKII 70 80 90 100 110 120 LTEEGKMYGR NELIARHIKL RTGKTRTRKQ VSSHIQVLAR RKAREIQAKL KFWQGALPGQ 130 140 150 160 170 180 PGTSHDVKPF SQNTYPVQPP LPLPGFESPA GPTPSPSAPL APPWQGRSIA SSKLWMLEFS 190 200 210 220 230 240 AFLERQQDPD TYNKHLFVHI SQSSPSYSDP YLETVDIRQI YDKFPEKKGG LKELFERGPS 250 260 270 280 290 300 NAFFLVKFWA DLNTNIDDEG SAFYGVSSQY ESPENMIITC STKVCSFGKQ VVEKVETEYA 310 320 330 340 350 360 RYENGHYLYR IHRSPLCEYM INFIHKLKHL PEKYMMNSVL ENFTILQVVT NRDTQETLLC 370 380 IAYVFEVSAS EHGAQHHIYR LVKE

References

[1]	"A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain." Jacquemin P., Hwang J.-J., Martial J.A., Dolle P., Davidson I. J. Biol. Chem. 271:21775-21785(1996) [PubMed: 8702974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A novel family of TEA domain-containing transcription factors with distinct spatiotemporal expression patterns." Yasunami M., Suzuki K., Ohkubo H. Biochem. Biophys. Res. Commun. 228:365-370(1996) [PubMed: 8920920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[3]	"cDNA cloning and characterization of murine transcriptional enhancer factor-1-related protein 1, a transcription factor that binds to the M-CAT motif." Yockey C.E., Smith G., Izumo S., Shimizu N. J. Biol. Chem. 271:3727-3736(1996) [PubMed: 8631987] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). Strain: C3H.
[4]	"Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation." Hsu D.K.W., Guo Y., Alberts G.F., Copeland N.G., Gilbert D.J., Jenkins N.A., Peifley K.A., Winkles J.A. J. Biol. Chem. 271:13786-13795(1996) [PubMed: 8662936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-427 (ISOFORMS LONG AND SHORT). Strain: BALB/c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94441 mRNA. Translation: CAA64215.2. D87966 mRNA. Translation: BAA13519.1. D87965 mRNA. Translation: BAA13518.1. L26343 mRNA. Translation: AAB12488.1. Different initiation. L26344 mRNA. Translation: AAC37680.1. U51743 mRNA. Translation: AAC52646.1. U51745 mRNA. Translation: AAC52647.1.
IPI	IPI00123584. IPI00264931.
PIR	JC5254. JC5255.
RefSeq	NP_001074448.2. NP_035697.3.
UniGene	Mm.14774
3D structure databases
SMR	Q62296. Positions 28-106.
ModBase	Search...
PTM databases
PhosphoSite	Q62296.
Genome annotation databases
Ensembl	ENSMUSG00000030353. Mus musculus. [Contig view]
GeneID	21679.
KEGG	mmu:21679.
Organism-specific databases
MGI	MGI:106907. Tead4.
Phylogenomic databases
HOGENOM	Q62296.
HOVERGEN	Q62296.
Gene expression databases
ArrayExpress	Q62296.
Bgee	Q62296.
CleanEx	MM_TEAD4.
GermOnline	ENSMUSG00000030353. Mus musculus.
Family and domain databases
InterPro	IPR000818. TEA/ATTS. IPR016361. TEF. [Graphical view]
PANTHER	PTHR11834. TEA/ATTS. 1 hit.
Pfam	PF01285. TEA. 1 hit. [Graphical view]
PIRSF	PIRSF002603. TEF. 1 hit.
PRINTS	PR00065. TEADOMAIN.
SMART	SM00426. TEA. 1 hit. [Graphical view]
PROSITE	PS00554. TEA_1. 1 hit. PS51088. TEA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	300982.
SOURCE	Search...

Entry information

Entry name

TEAD4_MOUSE

Accession

Primary (citable) accession number: Q62296
Secondary accession number(s): P70282 , Q61174, Q61175, Q62298

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)