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Protein

Transcriptional enhancer factor TEF-3

Gene

Tead4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif (By similarity). Might play a role in the embryonic development of skeletal muscle.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi31 – 9868TEAPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • blastocyst formation Source: MGI
  • cell fate commitment Source: MGI
  • cell fate specification Source: MGI
  • embryo implantation Source: MGI
  • hippo signaling Source: MGI
  • in utero embryonic development Source: MGI
  • positive regulation of defense response to virus by host Source: MGI
  • positive regulation of stem cell maintenance Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • trophectodermal cell fate commitment Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_287523. YAP1- and WWTR1 (TAZ)-stimulated gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional enhancer factor TEF-3
Alternative name(s):
ETF-related factor 2
Short name:
ETFR-2
TEA domain family member 4
Short name:
TEAD-4
TEF-1-related factor 1
TEF-1-related factor FR-19
Short name:
RTEF-1
Gene namesi
Name:Tead4
Synonyms:Tcf13r1, Tef3, Tefr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:106907. Tead4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • protein-DNA complex Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Transcriptional enhancer factor TEF-3PRO_0000205938Add
BLAST

Proteomic databases

PRIDEiQ62296.

PTM databases

PhosphoSiteiQ62296.

Expressioni

Tissue specificityi

Preferentially expressed in lung and in skeletal muscle.

Inductioni

By FGF-1, FGF-2, calf serum, platelet-derived growth factor-BB, and phorbol 12-myristate 13-acetate.

Gene expression databases

BgeeiQ62296.
CleanExiMM_TEAD4.
ExpressionAtlasiQ62296. baseline and differential.
GenevisibleiQ62296. MM.

Interactioni

Subunit structurei

Interacts with WWTR1/TAZ (By similarity). Interacts with YAP1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Vgll4Q80V243EBI-9253444,EBI-9253433

Protein-protein interaction databases

BioGridi204101. 3 interactions.
DIPiDIP-59847N.
IntActiQ62296. 2 interactions.
STRINGi10090.ENSMUSP00000006311.

Structurei

Secondary structure

1
427
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi210 – 2134Combined sources
Beta strandi218 – 2269Combined sources
Beta strandi239 – 2446Combined sources
Beta strandi250 – 2523Combined sources
Beta strandi257 – 2593Combined sources
Helixi260 – 2623Combined sources
Helixi264 – 2663Combined sources
Turni270 – 2723Combined sources
Helixi274 – 2807Combined sources
Beta strandi285 – 2939Combined sources
Helixi299 – 3013Combined sources
Beta strandi307 – 31711Combined sources
Beta strandi321 – 3299Combined sources
Beta strandi332 – 34110Combined sources
Beta strandi344 – 3463Combined sources
Beta strandi349 – 35810Combined sources
Helixi361 – 37010Combined sources
Beta strandi372 – 3743Combined sources
Helixi376 – 3838Combined sources
Beta strandi386 – 39712Combined sources
Beta strandi400 – 41011Combined sources
Beta strandi412 – 4154Combined sources
Beta strandi419 – 4257Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JUAX-ray3.00A/C/E/G210-427[»]
4EAZX-ray2.80A/B210-427[»]
4LN0X-ray2.90A/B209-427[»]
ProteinModelPortaliQ62296.
SMRiQ62296. Positions 28-106, 210-426.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62296.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi78 – 9417Nuclear localization signalSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 TEA DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG246992.
HOGENOMiHOG000253933.
HOVERGENiHBG056905.
InParanoidiQ62296.
KOiK09448.
OrthoDBiEOG7288RP.
PhylomeDBiQ62296.
TreeFamiTF313443.

Family and domain databases

InterProiIPR000818. TEA/ATTS.
IPR027255. TEF-3.
IPR016361. TEF_metazoa.
[Graphical view]
PANTHERiPTHR11834. PTHR11834. 1 hit.
PfamiPF01285. TEA. 1 hit.
[Graphical view]
PIRSFiPIRSF002603. TEF. 1 hit.
PIRSF500722. TEF-3. 1 hit.
PRINTSiPR00065. TEADOMAIN.
SMARTiSM00426. TEA. 1 hit.
[Graphical view]
PROSITEiPS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q62296-1) [UniParc]FASTAAdd to basket

Also known as: TEFR1A, ETFR-2A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSNEWSSPD SPEGSSISGG SQALDKPIDN DAEGVWSPEI ERSFQEALAI
60 70 80 90 100
YPPCGRRKII LTEEGKMYGR NELIARHIKL RTGKTRTRKQ VSSHIQVLAR
110 120 130 140 150
RKAREIQAKL KDQAAKNKAL QSMAAMSSAQ IVSATAFHSK MALARGPGYP
160 170 180 190 200
AISGFWQGAL PGQPGTSHDV KPFSQNTYPV QPPLPLPGFE SPAGPTPSPS
210 220 230 240 250
APLAPPWQGR SIASSKLWML EFSAFLERQQ DPDTYNKHLF VHISQSSPSY
260 270 280 290 300
SDPYLETVDI RQIYDKFPEK KGGLKELFER GPSNAFFLVK FWADLNTNID
310 320 330 340 350
DEGSAFYGVS SQYESPENMI ITCSTKVCSF GKQVVEKVET EYARYENGHY
360 370 380 390 400
LYRIHRSPLC EYMINFIHKL KHLPEKYMMN SVLENFTILQ VVTNRDTQET
410 420
LLCIAYVFEV SASEHGAQHH IYRLVKE
Length:427
Mass (Da):48,028
Last modified:November 1, 1997 - v2
Checksum:i8A0E9749840D88EC
GO
Isoform Short (identifier: Q62296-2) [UniParc]FASTAAdd to basket

Also known as: TEFR1B, ETFR-2B

The sequence of this isoform differs from the canonical sequence as follows:
     112-154: Missing.

Show »
Length:384
Mass (Da):43,710
Checksum:i07B8CFF2E0E3798B
GO

Sequence cautioni

The sequence AAB12488.1 differs from that shown.Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon.Curated
The sequence AAB12488.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti142 – 1421A → L in AAB12488 (PubMed:8631987).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei112 – 15443Missing in isoform Short. 4 PublicationsVSP_006390Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94441 mRNA. Translation: CAA64215.2.
D87966 mRNA. Translation: BAA13519.1.
D87965 mRNA. Translation: BAA13518.1.
L26343 mRNA. Translation: AAB12488.1. Sequence problems.
L26344 mRNA. Translation: AAC37680.1.
BC130257 mRNA. Translation: AAI30258.1.
U51743 mRNA. Translation: AAC52646.1.
U51745 mRNA. Translation: AAC52647.1.
CCDSiCCDS39645.1. [Q62296-2]
CCDS39646.1. [Q62296-1]
PIRiJC5254.
JC5255.
RefSeqiNP_001074448.2. NM_001080979.1. [Q62296-2]
NP_035697.3. NM_011567.2. [Q62296-1]
UniGeneiMm.14774.

Genome annotation databases

GeneIDi21679.
KEGGimmu:21679.
UCSCiuc009ede.1. mouse. [Q62296-1]
uc009edf.1. mouse. [Q62296-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94441 mRNA. Translation: CAA64215.2.
D87966 mRNA. Translation: BAA13519.1.
D87965 mRNA. Translation: BAA13518.1.
L26343 mRNA. Translation: AAB12488.1. Sequence problems.
L26344 mRNA. Translation: AAC37680.1.
BC130257 mRNA. Translation: AAI30258.1.
U51743 mRNA. Translation: AAC52646.1.
U51745 mRNA. Translation: AAC52647.1.
CCDSiCCDS39645.1. [Q62296-2]
CCDS39646.1. [Q62296-1]
PIRiJC5254.
JC5255.
RefSeqiNP_001074448.2. NM_001080979.1. [Q62296-2]
NP_035697.3. NM_011567.2. [Q62296-1]
UniGeneiMm.14774.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JUAX-ray3.00A/C/E/G210-427[»]
4EAZX-ray2.80A/B210-427[»]
4LN0X-ray2.90A/B209-427[»]
ProteinModelPortaliQ62296.
SMRiQ62296. Positions 28-106, 210-426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204101. 3 interactions.
DIPiDIP-59847N.
IntActiQ62296. 2 interactions.
STRINGi10090.ENSMUSP00000006311.

PTM databases

PhosphoSiteiQ62296.

Proteomic databases

PRIDEiQ62296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi21679.
KEGGimmu:21679.
UCSCiuc009ede.1. mouse. [Q62296-1]
uc009edf.1. mouse. [Q62296-2]

Organism-specific databases

CTDi7004.
MGIiMGI:106907. Tead4.

Phylogenomic databases

eggNOGiNOG246992.
HOGENOMiHOG000253933.
HOVERGENiHBG056905.
InParanoidiQ62296.
KOiK09448.
OrthoDBiEOG7288RP.
PhylomeDBiQ62296.
TreeFamiTF313443.

Enzyme and pathway databases

ReactomeiREACT_287523. YAP1- and WWTR1 (TAZ)-stimulated gene expression.

Miscellaneous databases

EvolutionaryTraceiQ62296.
NextBioi300982.
PROiQ62296.
SOURCEiSearch...

Gene expression databases

BgeeiQ62296.
CleanExiMM_TEAD4.
ExpressionAtlasiQ62296. baseline and differential.
GenevisibleiQ62296. MM.

Family and domain databases

InterProiIPR000818. TEA/ATTS.
IPR027255. TEF-3.
IPR016361. TEF_metazoa.
[Graphical view]
PANTHERiPTHR11834. PTHR11834. 1 hit.
PfamiPF01285. TEA. 1 hit.
[Graphical view]
PIRSFiPIRSF002603. TEF. 1 hit.
PIRSF500722. TEF-3. 1 hit.
PRINTSiPR00065. TEADOMAIN.
SMARTiSM00426. TEA. 1 hit.
[Graphical view]
PROSITEiPS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain."
    Jacquemin P., Hwang J.-J., Martial J.A., Dolle P., Davidson I.
    J. Biol. Chem. 271:21775-21785(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A novel family of TEA domain-containing transcription factors with distinct spatiotemporal expression patterns."
    Yasunami M., Suzuki K., Ohkubo H.
    Biochem. Biophys. Res. Commun. 228:365-370(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
  3. "cDNA cloning and characterization of murine transcriptional enhancer factor-1-related protein 1, a transcription factor that binds to the M-CAT motif."
    Yockey C.E., Smith G., Izumo S., Shimizu N.
    J. Biol. Chem. 271:3727-3736(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    Strain: C3H.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
  5. "Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation."
    Hsu D.K.W., Guo Y., Alberts G.F., Copeland N.G., Gilbert D.J., Jenkins N.A., Peifley K.A., Winkles J.A.
    J. Biol. Chem. 271:13786-13795(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-427 (ISOFORMS LONG AND SHORT).
    Strain: BALB/c.
  6. "Structural basis of YAP recognition by TEAD4 in the hippo pathway."
    Chen L., Chan S.W., Zhang X., Walsh M., Lim C.J., Hong W., Song H.
    Genes Dev. 24:290-300(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 210-427 IN COMPLEX WITH YAP1.

Entry informationi

Entry nameiTEAD4_MOUSE
AccessioniPrimary (citable) accession number: Q62296
Secondary accession number(s): A2BDD5
, P70282, Q61174, Q61175, Q62298
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.