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Q62296 (TEAD4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional enhancer factor TEF-3
Alternative name(s):
ETF-related factor 2
Short name=ETFR-2
TEA domain family member 4
Short name=TEAD-4
TEF-1-related factor 1
TEF-1-related factor FR-19
Short name=RTEF-1
Gene names
Name:Tead4
Synonyms:Tcf13r1, Tef3, Tefr1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif By similarity. Might play a role in the embryonic development of skeletal muscle.

Subunit structure

Interacts with WWTR1/TAZ By similarity. Interacts with YAP1.

Subcellular location

Nucleus.

Tissue specificity

Preferentially expressed in lung and in skeletal muscle.

Induction

By FGF-1, FGF-2, calf serum, platelet-derived growth factor-BB, and phorbol 12-myristate 13-acetate.

Sequence similarities

Contains 1 TEA DNA-binding domain.

Sequence caution

The sequence AAB12488.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAB12488.1 differs from that shown. Reason: Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Molecular functionActivator
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processblastocyst formation

Inferred from mutant phenotype PubMed 18804437. Source: MGI

cell fate specification

Inferred from mutant phenotype PubMed 18083014. Source: MGI

embryo implantation

Inferred from mutant phenotype PubMed 18083014. Source: MGI

hippo signaling

Inferred from electronic annotation. Source: InterPro

in utero embryonic development

Inferred from mutant phenotype PubMed 18083014. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 16207754. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 18083014. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

trophectodermal cell fate commitment

Inferred from mutant phenotype PubMed 18083014. Source: MGI

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

transcription factor complex

Inferred from direct assay PubMed 16207754. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 16207754Ref.1. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q62296-1)

Also known as: TEFR1A; ETFR-2A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q62296-2)

Also known as: TEFR1B; ETFR-2B;

The sequence of this isoform differs from the canonical sequence as follows:
     112-154: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Transcriptional enhancer factor TEF-3
PRO_0000205938

Regions

DNA binding31 – 9868TEA
Motif78 – 9417Nuclear localization signal Potential

Natural variations

Alternative sequence112 – 15443Missing in isoform Short.
VSP_006390

Experimental info

Sequence conflict1421A → L in AAB12488. Ref.3

Secondary structure

.............................................. 427
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Long (TEFR1A) (ETFR-2A) [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 8A0E9749840D88EC

FASTA42748,028
        10         20         30         40         50         60 
MTSNEWSSPD SPEGSSISGG SQALDKPIDN DAEGVWSPEI ERSFQEALAI YPPCGRRKII 

        70         80         90        100        110        120 
LTEEGKMYGR NELIARHIKL RTGKTRTRKQ VSSHIQVLAR RKAREIQAKL KDQAAKNKAL 

       130        140        150        160        170        180 
QSMAAMSSAQ IVSATAFHSK MALARGPGYP AISGFWQGAL PGQPGTSHDV KPFSQNTYPV 

       190        200        210        220        230        240 
QPPLPLPGFE SPAGPTPSPS APLAPPWQGR SIASSKLWML EFSAFLERQQ DPDTYNKHLF 

       250        260        270        280        290        300 
VHISQSSPSY SDPYLETVDI RQIYDKFPEK KGGLKELFER GPSNAFFLVK FWADLNTNID 

       310        320        330        340        350        360 
DEGSAFYGVS SQYESPENMI ITCSTKVCSF GKQVVEKVET EYARYENGHY LYRIHRSPLC 

       370        380        390        400        410        420 
EYMINFIHKL KHLPEKYMMN SVLENFTILQ VVTNRDTQET LLCIAYVFEV SASEHGAQHH 


IYRLVKE 

« Hide

Isoform Short (TEFR1B) (ETFR-2B) [UniParc].

Checksum: 07B8CFF2E0E3798B
Show »

FASTA38443,710

References

« Hide 'large scale' references
[1]"A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain."
Jacquemin P., Hwang J.-J., Martial J.A., Dolle P., Davidson I.
J. Biol. Chem. 271:21775-21785(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A novel family of TEA domain-containing transcription factors with distinct spatiotemporal expression patterns."
Yasunami M., Suzuki K., Ohkubo H.
Biochem. Biophys. Res. Commun. 228:365-370(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[3]"cDNA cloning and characterization of murine transcriptional enhancer factor-1-related protein 1, a transcription factor that binds to the M-CAT motif."
Yockey C.E., Smith G., Izumo S., Shimizu N.
J. Biol. Chem. 271:3727-3736(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
Strain: C3H.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
[5]"Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation."
Hsu D.K.W., Guo Y., Alberts G.F., Copeland N.G., Gilbert D.J., Jenkins N.A., Peifley K.A., Winkles J.A.
J. Biol. Chem. 271:13786-13795(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-427 (ISOFORMS LONG AND SHORT).
Strain: BALB/c.
[6]"Structural basis of YAP recognition by TEAD4 in the hippo pathway."
Chen L., Chan S.W., Zhang X., Walsh M., Lim C.J., Hong W., Song H.
Genes Dev. 24:290-300(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 210-427 IN COMPLEX WITH YAP1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X94441 mRNA. Translation: CAA64215.2.
D87966 mRNA. Translation: BAA13519.1.
D87965 mRNA. Translation: BAA13518.1.
L26343 mRNA. Translation: AAB12488.1. Sequence problems.
L26344 mRNA. Translation: AAC37680.1.
BC130257 mRNA. Translation: AAI30258.1.
U51743 mRNA. Translation: AAC52646.1.
U51745 mRNA. Translation: AAC52647.1.
CCDSCCDS39645.1. [Q62296-2]
CCDS39646.1. [Q62296-1]
PIRJC5254.
JC5255.
RefSeqNP_001074448.2. NM_001080979.1. [Q62296-2]
NP_035697.3. NM_011567.2. [Q62296-1]
UniGeneMm.14774.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3JUAX-ray3.00A/C/E/G210-427[»]
4EAZX-ray2.80A/B210-427[»]
4LN0X-ray2.90A/B209-427[»]
ProteinModelPortalQ62296.
SMRQ62296. Positions 28-106, 210-426.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204101. 3 interactions.
DIPDIP-59847N.

PTM databases

PhosphoSiteQ62296.

Proteomic databases

PRIDEQ62296.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006311; ENSMUSP00000006311; ENSMUSG00000030353.
ENSMUST00000112157; ENSMUSP00000107784; ENSMUSG00000030353.
ENSMUST00000130454; ENSMUSP00000118083; ENSMUSG00000030353.
GeneID21679.
KEGGmmu:21679.
UCSCuc009ede.1. mouse. [Q62296-1]
uc009edf.1. mouse. [Q62296-2]

Organism-specific databases

CTD7004.
MGIMGI:106907. Tead4.

Phylogenomic databases

eggNOGNOG246992.
GeneTreeENSGT00390000008670.
HOGENOMHOG000253933.
HOVERGENHBG056905.
InParanoidQ62296.
KOK09448.
OrthoDBEOG7288RP.
PhylomeDBQ62296.
TreeFamTF313443.

Gene expression databases

ArrayExpressQ62296.
BgeeQ62296.
CleanExMM_TEAD4.
GenevestigatorQ62296.

Family and domain databases

InterProIPR000818. TEA/ATTS.
IPR016361. TEF.
IPR027255. TEF-3.
[Graphical view]
PANTHERPTHR11834. PTHR11834. 1 hit.
PfamPF01285. TEA. 1 hit.
[Graphical view]
PIRSFPIRSF002603. TEF. 1 hit.
PIRSF500722. TEF-3. 1 hit.
PRINTSPR00065. TEADOMAIN.
SMARTSM00426. TEA. 1 hit.
[Graphical view]
PROSITEPS00554. TEA_1. 1 hit.
PS51088. TEA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ62296.
NextBio300982.
PROQ62296.
SOURCESearch...

Entry information

Entry nameTEAD4_MOUSE
AccessionPrimary (citable) accession number: Q62296
Secondary accession number(s): A2BDD5 expand/collapse secondary AC list , P70282, Q61174, Q61175, Q62298
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot