Reviewed, UniProtKB/Swiss-Prot Q62277 (SYPH_MOUSE)

Names and origin

Protein names

Recommended name:
Synaptophysin

Alternative name(s):
Major synaptic vesicle protein p38
BM89 antigen

Gene names

Name:

Syp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane By similarity. Involved in the regulation of short-term and long-term synaptic plasticity. Ref.5
Subunit structure	Homohexamer or homotetramer By similarity.
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Multi-pass membrane protein By similarity. Cell junction › synapse › synaptosome By similarity.
Domain	The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
Post-translational modification	Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation By similarity.
Disruption phenotype	Mice lackin both SYNGR1 and SYP show normal brain structure and composition, but impaired short-term and long-term synaptic plasticity. Ref.5
Sequence similarities	Belongs to the synaptophysin/synaptobrevin family. Contains 1 MARVEL domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome
Domain	Repeat Transmembrane
Ligand	Calcium
PTM	Glycoprotein Phosphoprotein Ubl conjugation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	endocytosis Traceable author statement. Source: MGI regulation of long-term neuronal synaptic plasticity Ref.5 Inferred from mutant phenotype. Source: UniProtKB regulation of short-term neuronal synaptic plasticity Ref.5 Inferred from mutant phenotype. Source: UniProtKB synaptic transmission Traceable author statement. Source: MGI
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to synaptic vesicle membrane Traceable author statement. Source: MGI presynaptic membrane Inferred from direct assay. Source: MGI synaptosome Inferred from direct assay. Source: MGI
Molecular function	SH2 domain binding Inferred from direct assay. Source: MGI calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: MGI transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Synaptophysin		PRO_0000179162
Regions
	Topological domain	1 – 25	25	Cytoplasmic Potential
	Transmembrane	26 – 49	24	Potential
	Topological domain	50 – 107	58	Vesicular Potential
	Transmembrane	108 – 131	24	Potential
	Topological domain	132 – 138	7	Cytoplasmic Potential
	Transmembrane	139 – 162	24	Potential
	Topological domain	163 – 200	38	Vesicular Potential
	Transmembrane	201 – 224	24	Potential
	Topological domain	225 – 314	90	Cytoplasmic Potential
	Domain	21 – 228	208	MARVEL
	Region	255 – 305	51	Repeats, Gly-rich
Amino acid modifications
	Modified residue	81	1	Phosphotyrosine Ref.6
	Modified residue	227	1	Phosphothreonine Potential
	Modified residue	279	1	Phosphotyrosine Potential
	Modified residue	296	1	Phosphotyrosine Potential
	Glycosylation	59	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	105	1	A → G in CAA65084. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q62277-1 [UniParc]. Last modified October 10, 2003. Version 2. Checksum: F6AC6E30AECE10CE	FASTA	314	34,025
	10 20 30 40 50 60 MLLLADMDVV NQLVAGGQFR VVKEPLGFVK VLQWVFAIFA FATCGSYTGE LRLSVECANK 70 80 90 100 110 120 TESALNIEVE FEYPFRLHQV YFDAPSCVKG GTTKIFLVGD YSSSAEFFVT VAVFAFLYSM 130 140 150 160 170 180 GALATYIFLQ NKYRENNKGP MMDFLATAVF AFMWLVSSSA WAKGLSDVKM ATDPENIIKE 190 200 210 220 230 240 MPMCRQTGNT CKELRDPVTS GLNTSVVFGF LNLVLWVGNL WFVFKETGWA APFMRAPPGA 250 260 270 280 290 300 PEKQPAPGDA YGDAGYGQGP GGYGPQDSYG PQGGYQPDYG QPASGGGGGY GPQGDYGQQG 310 YGQQGAPTSF SNQM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Brain cortex.
[2]	"Purification and cDNA cloning of mouse BM89 antigen shows that it is identical with the synaptic vesicle protein synaptophysin." Gaitanou M., Mamalaki A., Merkouri E., Matsas R. J. Neurosci. Res. 48:507-514(1997) [PubMed: 9210520] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-314. Strain: C57BL/6. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-314. Tissue: Eye.
[4]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 77-89; 170-179 AND 226-235, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[5]	"Essential roles in synaptic plasticity for synaptogyrin I and synaptophysin I." Janz R., Suedhof T.C., Hammer R.E., Unni V., Siegelbaum S.A., Bolshakov V.Y. Neuron 24:687-700(1999) [PubMed: 10595519] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-81, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK043756 mRNA. Translation: BAC31642.1. X95818 mRNA. Translation: CAA65084.1. BC014823 mRNA. Translation: AAH14823.1.
IPI	IPI00123505.
RefSeq	NP_033331.2.
UniGene	Mm.223674
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62277. 3 interactions.
STRING	Q62277.
PTM databases
PhosphoSite	Q62277.
Proteomic databases
PRIDE	Q62277.
Genome annotation databases
Ensembl	ENSMUST00000069520; ENSMUSP00000069429; ENSMUSG00000031144; Mus musculus. [Genome view] ENSMUST00000116638; ENSMUSP00000112337; ENSMUSG00000031144; Mus musculus. [Genome view]
GeneID	20977.
KEGG	mmu:20977.
UCSC	uc009sls.1. mouse.
Organism-specific databases
CTD	20977.
MGI	MGI:98467. Syp.
Phylogenomic databases
HOGENOM	HBG714484.
HOVERGEN	HBG006681.
InParanoid	Q62277.
OMA	TGNTCKE.
OrthoDB	EOG976NJX.
PhylomeDB	Q62277.
Gene expression databases
ArrayExpress	Q62277.
Bgee	Q62277.
CleanEx	MM_SYP.
Genevestigator	Q62277.
GermOnline	ENSMUSG00000031144. Mus musculus.
Family and domain databases
InterPro	IPR008253. Marvel. IPR021128. MARVEL-like_dom. IPR001285. Synaptophysin/porin. IPR015457. Synaptophysin_. [Graphical view]
PANTHER	PTHR10306. Synaptophysin. 1 hit. PTHR10306:SF2. Synaptophysin_. 1 hit.
Pfam	PF01284. MARVEL. 1 hit. [Graphical view]
PRINTS	PR00220. SYNAPTOPHYSN.
PROSITE	PS51225. MARVEL. 1 hit. PS00604. SYNAPTOP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	299960.
SOURCE	Search...

Entry information

Entry name

SYPH_MOUSE

Accession

Primary (citable) accession number: Q62277
Secondary accession number(s): Q8BRQ0, Q91WI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 10, 2003
Last modified:	March 2, 2010
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)