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Protein

Synaptophysin

Gene

Syp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane (By similarity). Involved in the regulation of short-term and long-term synaptic plasticity.By similarity1 Publication

GO - Molecular functioni

  1. cholesterol binding Source: MGI
  2. identical protein binding Source: MGI
  3. SH2 domain binding Source: MGI
  4. syntaxin-1 binding Source: MGI
  5. transporter activity Source: InterPro

GO - Biological processi

  1. cellular response to organic substance Source: MGI
  2. endocytosis Source: UniProtKB
  3. regulation of long-term neuronal synaptic plasticity Source: UniProtKB
  4. regulation of opioid receptor signaling pathway Source: UniProtKB
  5. regulation of short-term neuronal synaptic plasticity Source: UniProtKB
  6. synaptic transmission Source: MGI
Complete GO annotation...

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptophysin
Alternative name(s):
BM89 antigen
Major synaptic vesicle protein p38
Gene namesi
Name:Syp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:98467. Syp.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei26 – 4924HelicalSequence AnalysisAdd
BLAST
Topological domaini50 – 10758VesicularSequence AnalysisAdd
BLAST
Transmembranei108 – 13124HelicalSequence AnalysisAdd
BLAST
Topological domaini132 – 1387CytoplasmicSequence Analysis
Transmembranei139 – 16224HelicalSequence AnalysisAdd
BLAST
Topological domaini163 – 20038VesicularSequence AnalysisAdd
BLAST
Transmembranei201 – 22424HelicalSequence AnalysisAdd
BLAST
Topological domaini225 – 31490CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. excitatory synapse Source: MGI
  3. integral component of synaptic vesicle membrane Source: GO_Central
  4. membrane Source: MGI
  5. neuron projection Source: ParkinsonsUK-UCL
  6. neuron projection terminus Source: MGI
  7. presynaptic active zone Source: BHF-UCL
  8. presynaptic membrane Source: UniProtKB
  9. protein complex Source: Ensembl
  10. synapse Source: MGI
  11. synaptic vesicle Source: MGI
  12. synaptic vesicle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Disruption phenotypei

Mice lackin both SYNGR1 and SYP show normal brain structure and composition, but impaired short-term and long-term synaptic plasticity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314SynaptophysinPRO_0000179162Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence Analysis
Modified residuei81 – 811Phosphotyrosine1 Publication
Modified residuei227 – 2271PhosphothreonineSequence Analysis
Modified residuei279 – 2791PhosphotyrosineSequence Analysis
Modified residuei296 – 2961PhosphotyrosineSequence Analysis

Post-translational modificationi

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ62277.
PaxDbiQ62277.
PRIDEiQ62277.

PTM databases

PhosphoSiteiQ62277.

Expressioni

Gene expression databases

BgeeiQ62277.
CleanExiMM_SYP.
GenevestigatoriQ62277.

Interactioni

Subunit structurei

Homohexamer or homotetramer. Interacts with SRCIN1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi203609. 11 interactions.
IntActiQ62277. 19 interactions.
MINTiMINT-4136497.

Structurei

3D structure databases

ProteinModelPortaliQ62277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 228208MARVELPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni255 – 30551Repeats, Gly-richAdd
BLAST

Domaini

The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.

Sequence similaritiesi

Belongs to the synaptophysin/synaptobrevin family.Curated
Contains 1 MARVEL domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG82723.
GeneTreeiENSGT00390000010039.
HOGENOMiHOG000252913.
HOVERGENiHBG006681.
InParanoidiQ62277.
OMAiAYGDAGY.
OrthoDBiEOG7WT43S.
PhylomeDBiQ62277.
TreeFamiTF315804.

Family and domain databases

InterProiIPR008253. Marvel.
IPR001285. Synaptophysin/porin.
IPR028714. SYP.
[Graphical view]
PANTHERiPTHR10306. PTHR10306. 1 hit.
PTHR10306:SF10. PTHR10306:SF10. 1 hit.
PfamiPF01284. MARVEL. 1 hit.
[Graphical view]
PRINTSiPR00220. SYNAPTOPHYSN.
PROSITEiPS51225. MARVEL. 1 hit.
PS00604. SYNAPTOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62277-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLLADMDVV NQLVAGGQFR VVKEPLGFVK VLQWVFAIFA FATCGSYTGE
60 70 80 90 100
LRLSVECANK TESALNIEVE FEYPFRLHQV YFDAPSCVKG GTTKIFLVGD
110 120 130 140 150
YSSSAEFFVT VAVFAFLYSM GALATYIFLQ NKYRENNKGP MMDFLATAVF
160 170 180 190 200
AFMWLVSSSA WAKGLSDVKM ATDPENIIKE MPMCRQTGNT CKELRDPVTS
210 220 230 240 250
GLNTSVVFGF LNLVLWVGNL WFVFKETGWA APFMRAPPGA PEKQPAPGDA
260 270 280 290 300
YGDAGYGQGP GGYGPQDSYG PQGGYQPDYG QPASGGGGGY GPQGDYGQQG
310
YGQQGAPTSF SNQM
Length:314
Mass (Da):34,025
Last modified:October 10, 2003 - v2
Checksum:iF6AC6E30AECE10CE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051A → G in CAA65084. (PubMed:9210520)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK043756 mRNA. Translation: BAC31642.1.
X95818 mRNA. Translation: CAA65084.1.
BC014823 mRNA. Translation: AAH14823.1.
CCDSiCCDS29967.2.
RefSeqiNP_033331.2. NM_009305.2.
UniGeneiMm.223674.

Genome annotation databases

EnsembliENSMUST00000069520; ENSMUSP00000069429; ENSMUSG00000031144.
GeneIDi20977.
KEGGimmu:20977.
UCSCiuc009slr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK043756 mRNA. Translation: BAC31642.1.
X95818 mRNA. Translation: CAA65084.1.
BC014823 mRNA. Translation: AAH14823.1.
CCDSiCCDS29967.2.
RefSeqiNP_033331.2. NM_009305.2.
UniGeneiMm.223674.

3D structure databases

ProteinModelPortaliQ62277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203609. 11 interactions.
IntActiQ62277. 19 interactions.
MINTiMINT-4136497.

PTM databases

PhosphoSiteiQ62277.

Proteomic databases

MaxQBiQ62277.
PaxDbiQ62277.
PRIDEiQ62277.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000069520; ENSMUSP00000069429; ENSMUSG00000031144.
GeneIDi20977.
KEGGimmu:20977.
UCSCiuc009slr.2. mouse.

Organism-specific databases

CTDi6855.
MGIiMGI:98467. Syp.

Phylogenomic databases

eggNOGiNOG82723.
GeneTreeiENSGT00390000010039.
HOGENOMiHOG000252913.
HOVERGENiHBG006681.
InParanoidiQ62277.
OMAiAYGDAGY.
OrthoDBiEOG7WT43S.
PhylomeDBiQ62277.
TreeFamiTF315804.

Miscellaneous databases

ChiTaRSiSyp. mouse.
NextBioi299960.
PROiQ62277.
SOURCEiSearch...

Gene expression databases

BgeeiQ62277.
CleanExiMM_SYP.
GenevestigatoriQ62277.

Family and domain databases

InterProiIPR008253. Marvel.
IPR001285. Synaptophysin/porin.
IPR028714. SYP.
[Graphical view]
PANTHERiPTHR10306. PTHR10306. 1 hit.
PTHR10306:SF10. PTHR10306:SF10. 1 hit.
PfamiPF01284. MARVEL. 1 hit.
[Graphical view]
PRINTSiPR00220. SYNAPTOPHYSN.
PROSITEiPS51225. MARVEL. 1 hit.
PS00604. SYNAPTOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain cortex.
  2. "Purification and cDNA cloning of mouse BM89 antigen shows that it is identical with the synaptic vesicle protein synaptophysin."
    Gaitanou M., Mamalaki A., Merkouri E., Matsas R.
    J. Neurosci. Res. 48:507-514(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-314.
    Strain: C57BL/6.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-314.
    Tissue: Eye.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 77-89; 170-179 AND 226-235, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  5. "Essential roles in synaptic plasticity for synaptogyrin I and synaptophysin I."
    Janz R., Suedhof T.C., Hammer R.E., Unni V., Siegelbaum S.A., Bolshakov V.Y.
    Neuron 24:687-700(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-81, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiSYPH_MOUSE
AccessioniPrimary (citable) accession number: Q62277
Secondary accession number(s): Q8BRQ0, Q91WI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 10, 2003
Last modified: February 4, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.