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Protein

Mediator of RNA polymerase II transcription subunit 22

Gene

Med22

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-442533 Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 22
Alternative name(s):
Mediator complex subunit 22
Surfeit locus protein 5
Short name:
Surf-5
Gene namesi
Name:Med22
Synonyms:Surf5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:98446 Med22

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001788391 – 200Mediator of RNA polymerase II transcription subunit 22Add BLAST200

Proteomic databases

PaxDbiQ62276
PRIDEiQ62276

PTM databases

iPTMnetiQ62276
PhosphoSitePlusiQ62276

Expressioni

Gene expression databases

BgeeiENSMUSG00000015776
CleanExiMM_MED22
ExpressionAtlasiQ62276 baseline and differential
GenevisibleiQ62276 MM

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Protein-protein interaction databases

BioGridi203582, 33 interactors
IntActiQ62276, 33 interactors
STRINGi10090.ENSMUSP00000015920

Structurei

3D structure databases

ProteinModelPortaliQ62276
SMRiQ62276
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili93 – 122Sequence analysisAdd BLAST30

Sequence similaritiesi

Belongs to the Mediator complex subunit 22 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3304 Eukaryota
ENOG410XS6U LUCA
GeneTreeiENSGT00390000004339
HOGENOMiHOG000006377
HOVERGENiHBG058487
InParanoidiQ62276
KOiK15139
OMAiKQAECDQ
OrthoDBiEOG091G0SIM
PhylomeDBiQ62276
TreeFamiTF323390

Family and domain databases

Gene3Di1.20.58.1600, 1 hit
InterProiView protein in InterPro
IPR009332 Med22
IPR038427 MED22_sf
PANTHERiPTHR12434 PTHR12434, 1 hit
PfamiView protein in Pfam
PF06179 Med22, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62276-1) [UniParc]FASTAAdd to basket
Also known as: Surf5B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR
60 70 80 90 100
ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ
110 120 130 140 150
RNQQLRALQE ECDRKLITLR DEVSIDLYEL EEEYYSSSSS LCEANDLPLC
160 170 180 190 200
EAYWRLDLDA DSADGLSAPL LASPETGAGP LQSAAPVHSH GGGPGPTEHT
Length:200
Mass (Da):22,282
Last modified:October 23, 2007 - v2
Checksum:iAF3802A645AE5FC2
GO
Isoform 2 (identifier: Q62276-2) [UniParc]FASTAAdd to basket
Also known as: Surf5A

The sequence of this isoform differs from the canonical sequence as follows:
     138-140: SSS → RYK
     141-200: Missing.

Show »
Length:140
Mass (Da):16,436
Checksum:i8D79D73D81171AE1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028993138 – 140SSS → RYK in isoform 2. 2 Publications3
Alternative sequenceiVSP_028994141 – 200Missing in isoform 2. 2 PublicationsAdd BLAST60

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85169 Genomic DNA Translation: CAA59453.1
AK144744 mRNA Translation: BAE26044.1
AK161053 mRNA Translation: BAE36173.1
AK166250 mRNA Translation: BAE38661.1
AK166474 mRNA Translation: BAE38795.1
AL773563 Genomic DNA Translation: CAM23699.1
AL773563 Genomic DNA Translation: CAM23700.1
BC018225 mRNA Translation: AAH18225.1
CCDSiCCDS15813.1 [Q62276-1]
CCDS15814.1 [Q62276-2]
RefSeqiNP_001029080.1, NM_001033908.1 [Q62276-1]
NP_035643.1, NM_011513.2 [Q62276-2]
UniGeneiMm.397932

Genome annotation databases

EnsembliENSMUST00000015920; ENSMUSP00000015920; ENSMUSG00000015776 [Q62276-1]
ENSMUST00000102899; ENSMUSP00000099963; ENSMUSG00000015776 [Q62276-2]
GeneIDi20933
KEGGimmu:20933
UCSCiuc008iwd.1 mouse [Q62276-1]
uc008iwe.1 mouse [Q62276-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMED22_MOUSE
AccessioniPrimary (citable) accession number: Q62276
Secondary accession number(s): A2ALA2, Q3TTZ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 23, 2007
Last modified: March 28, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health