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Protein

Mediator of RNA polymerase II transcription subunit 22

Gene

Med22

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 22
Alternative name(s):
Mediator complex subunit 22
Surfeit locus protein 5
Short name:
Surf-5
Gene namesi
Name:Med22
Synonyms:Surf5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:98446. Med22.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Mediator of RNA polymerase II transcription subunit 22PRO_0000178839Add
BLAST

Proteomic databases

MaxQBiQ62276.
PaxDbiQ62276.
PRIDEiQ62276.

PTM databases

iPTMnetiQ62276.
PhosphoSiteiQ62276.

Expressioni

Gene expression databases

BgeeiENSMUSG00000015776.
CleanExiMM_MED22.
ExpressionAtlasiQ62276. baseline and differential.
GenevisibleiQ62276. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Protein-protein interaction databases

BioGridi203582. 33 interactions.
IntActiQ62276. 33 interactions.
MINTiMINT-1666604.
STRINGi10090.ENSMUSP00000015920.

Structurei

3D structure databases

ProteinModelPortaliQ62276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili93 – 12230Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 22 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3304. Eukaryota.
ENOG410XS6U. LUCA.
GeneTreeiENSGT00390000004339.
HOGENOMiHOG000006377.
HOVERGENiHBG058487.
InParanoidiQ62276.
KOiK15139.
OMAiQNYNKRL.
OrthoDBiEOG091G0SIM.
PhylomeDBiQ62276.
TreeFamiTF323390.

Family and domain databases

InterProiIPR009332. Med22.
[Graphical view]
PANTHERiPTHR12434. PTHR12434. 1 hit.
PfamiPF06179. Med22. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62276-1) [UniParc]FASTAAdd to basket
Also known as: Surf5B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR
60 70 80 90 100
ATQGEQDNYE MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ
110 120 130 140 150
RNQQLRALQE ECDRKLITLR DEVSIDLYEL EEEYYSSSSS LCEANDLPLC
160 170 180 190 200
EAYWRLDLDA DSADGLSAPL LASPETGAGP LQSAAPVHSH GGGPGPTEHT
Length:200
Mass (Da):22,282
Last modified:October 23, 2007 - v2
Checksum:iAF3802A645AE5FC2
GO
Isoform 2 (identifier: Q62276-2) [UniParc]FASTAAdd to basket
Also known as: Surf5A

The sequence of this isoform differs from the canonical sequence as follows:
     138-140: SSS → RYK
     141-200: Missing.

Show »
Length:140
Mass (Da):16,436
Checksum:i8D79D73D81171AE1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei138 – 1403SSS → RYK in isoform 2. 2 PublicationsVSP_028993
Alternative sequencei141 – 20060Missing in isoform 2. 2 PublicationsVSP_028994Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85169 Genomic DNA. Translation: CAA59453.1.
AK144744 mRNA. Translation: BAE26044.1.
AK161053 mRNA. Translation: BAE36173.1.
AK166250 mRNA. Translation: BAE38661.1.
AK166474 mRNA. Translation: BAE38795.1.
AL773563 Genomic DNA. Translation: CAM23699.1.
AL773563 Genomic DNA. Translation: CAM23700.1.
BC018225 mRNA. Translation: AAH18225.1.
CCDSiCCDS15813.1. [Q62276-1]
CCDS15814.1. [Q62276-2]
RefSeqiNP_001029080.1. NM_001033908.1. [Q62276-1]
NP_035643.1. NM_011513.2. [Q62276-2]
UniGeneiMm.397932.

Genome annotation databases

EnsembliENSMUST00000015920; ENSMUSP00000015920; ENSMUSG00000015776. [Q62276-1]
ENSMUST00000102899; ENSMUSP00000099963; ENSMUSG00000015776. [Q62276-2]
GeneIDi20933.
KEGGimmu:20933.
UCSCiuc008iwd.1. mouse. [Q62276-1]
uc008iwe.1. mouse. [Q62276-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85169 Genomic DNA. Translation: CAA59453.1.
AK144744 mRNA. Translation: BAE26044.1.
AK161053 mRNA. Translation: BAE36173.1.
AK166250 mRNA. Translation: BAE38661.1.
AK166474 mRNA. Translation: BAE38795.1.
AL773563 Genomic DNA. Translation: CAM23699.1.
AL773563 Genomic DNA. Translation: CAM23700.1.
BC018225 mRNA. Translation: AAH18225.1.
CCDSiCCDS15813.1. [Q62276-1]
CCDS15814.1. [Q62276-2]
RefSeqiNP_001029080.1. NM_001033908.1. [Q62276-1]
NP_035643.1. NM_011513.2. [Q62276-2]
UniGeneiMm.397932.

3D structure databases

ProteinModelPortaliQ62276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203582. 33 interactions.
IntActiQ62276. 33 interactions.
MINTiMINT-1666604.
STRINGi10090.ENSMUSP00000015920.

PTM databases

iPTMnetiQ62276.
PhosphoSiteiQ62276.

Proteomic databases

MaxQBiQ62276.
PaxDbiQ62276.
PRIDEiQ62276.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015920; ENSMUSP00000015920; ENSMUSG00000015776. [Q62276-1]
ENSMUST00000102899; ENSMUSP00000099963; ENSMUSG00000015776. [Q62276-2]
GeneIDi20933.
KEGGimmu:20933.
UCSCiuc008iwd.1. mouse. [Q62276-1]
uc008iwe.1. mouse. [Q62276-2]

Organism-specific databases

CTDi6837.
MGIiMGI:98446. Med22.

Phylogenomic databases

eggNOGiKOG3304. Eukaryota.
ENOG410XS6U. LUCA.
GeneTreeiENSGT00390000004339.
HOGENOMiHOG000006377.
HOVERGENiHBG058487.
InParanoidiQ62276.
KOiK15139.
OMAiQNYNKRL.
OrthoDBiEOG091G0SIM.
PhylomeDBiQ62276.
TreeFamiTF323390.

Enzyme and pathway databases

ReactomeiR-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

PROiQ62276.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015776.
CleanExiMM_MED22.
ExpressionAtlasiQ62276. baseline and differential.
GenevisibleiQ62276. MM.

Family and domain databases

InterProiIPR009332. Med22.
[Graphical view]
PANTHERiPTHR12434. PTHR12434. 1 hit.
PfamiPF06179. Med22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED22_MOUSE
AccessioniPrimary (citable) accession number: Q62276
Secondary accession number(s): A2ALA2, Q3TTZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 23, 2007
Last modified: September 7, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.