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Protein

Tyrosine-protein kinase Srms

Gene

Srms

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues. May be involved in proliferation or differentiation of keratinocytes in the skin.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei262 – 2621ATPPROSITE-ProRule annotation
Active sitei354 – 3541Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi240 – 2489ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: GO_Central
  3. protein tyrosine kinase activity Source: UniProtKB
  4. receptor binding Source: GO_Central

GO - Biological processi

  1. actin cytoskeleton organization Source: GO_Central
  2. cell differentiation Source: GO_Central
  3. cell migration Source: GO_Central
  4. innate immune response Source: GO_Central
  5. peptidyl-tyrosine autophosphorylation Source: UniProtKB
  6. regulation of cell proliferation Source: GO_Central
  7. transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Srms (EC:2.7.10.2)
Alternative name(s):
PTK70
Gene namesi
Name:Srms
Synonyms:Srm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:101865. Srms.

Subcellular locationi

  1. Cytoplasm By similarity

  2. Note: Localizes to punctate cytoplasmic structures.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Tyrosine-protein kinase SrmsPRO_0000088161Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei384 – 3841Phosphotyrosine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ62270.

PTM databases

PhosphoSiteiQ62270.

Expressioni

Gene expression databases

BgeeiQ62270.
CleanExiMM_SRM.
ExpressionAtlasiQ62270. baseline and differential.
GenevestigatoriQ62270.

Interactioni

Subunit structurei

Interacts (via the SH2 and SH3 domains) with DOK1.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ62270.
SMRiQ62270. Positions 60-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 11662SH3PROSITE-ProRule annotationAdd
BLAST
Domaini124 – 21693SH2PROSITE-ProRule annotationAdd
BLAST
Domaini234 – 495262Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 5555N-terminalBy similarityAdd
BLAST

Domaini

The N-terminal region regulates its kinase activity.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
HOVERGENiHBG008761.
InParanoidiQ62270.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPFLRKRLT FLSFFWDKIW PADESEEDIP RIQGHDDNPV PEQAAAVEPC
60 70 80 90 100
SFPAPRARLF RALYDFTARC AEELSVSRGD RLYALKEEGD YIFAQRLSGP
110 120 130 140 150
PSTGLVPVTY LAKATPEPPS DQPWYFSGIS RAQAQQLLLS PANAPGAFLI
160 170 180 190 200
RPSESSIGGY SLSVRAQAKV CHYRICMAPS GSLYLQEGQL FPSLDALLAY
210 220 230 240 250
YKTNWKLIQN PLLQPCIPQI PLVQDEWERP RSEFVLRRKL GEGFFGEVWE
260 270 280 290 300
GLWLGSIPVA VKVIKSADMK LADLTKEIEA LKSLRHERLI RLHAICSLGE
310 320 330 340 350
PVYIVTELMG KGNLQVYLGS SEGKALSLPH LLGFACQVAE GMSYLEERRV
360 370 380 390 400
VHRDLAARNV LVGDDLTCKV ADFGLARLLK DDVYSPSSGS KIPVKWTAPE
410 420 430 440 450
AANYRVFSQK SDVWSFGILL YEVFTYGQCP YEGMTNHETL QQISRGYRLP
460 470 480 490
RPAVCPAEVY VLMVECWKGS PEERPTFAIL REKLNAINRR LHLGLT
Length:496
Mass (Da):55,758
Last modified:January 17, 2003 - v2
Checksum:i7FE39C9912219BAF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781R → G in BAA05331 (PubMed:7935409).Curated
Sequence conflicti236 – 2372LR → FG in BAA05331 (PubMed:7935409).Curated
Sequence conflicti238 – 2381R → K in BAA08406 (PubMed:9226137).Curated
Sequence conflicti278 – 2781I → N in BAA08406 (PubMed:9226137).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49427 mRNA. Translation: BAA08406.1.
D26186 mRNA. Translation: BAA05331.1.
AL450341 Genomic DNA. No translation available.
PIRiA56040.
UniGeneiMm.4752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49427 mRNA. Translation: BAA08406.1.
D26186 mRNA. Translation: BAA05331.1.
AL450341 Genomic DNA. No translation available.
PIRiA56040.
UniGeneiMm.4752.

3D structure databases

ProteinModelPortaliQ62270.
SMRiQ62270. Positions 60-481.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ62270.

Proteomic databases

PRIDEiQ62270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:101865. Srms.

Phylogenomic databases

eggNOGiCOG0515.
HOVERGENiHBG008761.
InParanoidiQ62270.

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.

Miscellaneous databases

PROiQ62270.
SOURCEiSearch...

Gene expression databases

BgeeiQ62270.
CleanExiMM_SRM.
ExpressionAtlasiQ62270. baseline and differential.
GenevestigatoriQ62270.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of a cDNA encoding a tyrosine kinase expressed in murine skin."
    Kawachi Y., Nakauchi H., Otsuka F.
    Exp. Dermatol. 6:140-146(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Thymus.
  2. "A novel nonreceptor tyrosine kinase, Srm: cloning and targeted disruption."
    Kohmura N., Yagi T., Tomooka Y., Oyanagi M., Kominami R., Takeda N., Chiba J., Ikawa Y., Aizawa S.
    Mol. Cell. Biol. 14:6915-6925(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lung.
  3. Wall M.
    Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiSRMS_MOUSE
AccessioniPrimary (citable) accession number: Q62270
Secondary accession number(s): Q62360, Q923M5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 17, 2003
Last modified: February 4, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.