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Protein

Thyroid hormone-inducible hepatic protein

Gene

Thrsp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of lipogenesis, especially in lactating mammary gland. Important for the biosynthesis of triglycerides with medium-length fatty acid chains. May modulate lipogenesis by interacting with MID1IP1 and preventing its interaction with ACACA. May function as transcriptional coactivator. May modulate the transcription factor activity of THRB (By similarity).By similarity2 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • lipid metabolic process Source: UniProtKB-KW
  • regulation of lipid biosynthetic process Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • regulation of triglyceride biosynthetic process Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Thyroid hormone-inducible hepatic protein
Alternative name(s):
Spot 14 protein
Short name:
S14
Short name:
SPOT14
Gene namesi
Name:Thrsp
Synonyms:S14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:109126. Thrsp.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Decreased lipid synthesis in the lactating mammary gland. Milk has reduced triglyceride content, causing reduced weight gain in nursing pups. Adults exhibit reduced body fat content. No effect on lipid synthesis in liver. No effect on the expression of thyroid hormone-induced lipogenic genes.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Thyroid hormone-inducible hepatic proteinPRO_0000123774Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei90 – 901PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ62264.
MaxQBiQ62264.
PaxDbiQ62264.
PRIDEiQ62264.

PTM databases

iPTMnetiQ62264.
SwissPalmiQ62264.

Expressioni

Tissue specificityi

Mainly expressed in tissues that synthesize triglycerides.1 Publication

Gene expression databases

BgeeiQ62264.
ExpressionAtlasiQ62264. baseline and differential.
GenevisibleiQ62264. MM.

Interactioni

Subunit structurei

Homodimer. Heterodimer with MID1IP1. Interacts with THRB and PLAGL1 (By similarity).By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-59527N.
STRINGi10090.ENSMUSP00000042988.

Structurei

Secondary structure

1
150
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 3018Combined sources
Helixi34 – 374Combined sources
Beta strandi51 – 533Combined sources
Helixi56 – 7116Combined sources
Helixi108 – 1114Combined sources
Helixi113 – 14533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ONTX-ray2.65A1-150[»]
ProteinModelPortaliQ62264.
SMRiQ62264. Positions 8-149.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62264.

Family & Domainsi

Sequence similaritiesi

Belongs to the SPOT14 family.Curated

Phylogenomic databases

eggNOGiENOG410J34G. Eukaryota.
ENOG410YT7Q. LUCA.
GeneTreeiENSGT00500000044890.
HOGENOMiHOG000001157.
HOVERGENiHBG002528.
InParanoidiQ62264.
OMAiNMEQVVM.
OrthoDBiEOG7CZK87.
PhylomeDBiQ62264.
TreeFamiTF326826.

Family and domain databases

InterProiIPR009786. Spot_14.
[Graphical view]
PANTHERiPTHR14315. PTHR14315. 1 hit.
PfamiPF07084. Spot_14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q62264-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVLTKRYPK NCLLTVMDRY SAVVRNMEQV VMIPSLLRDV QLSGPGGSVQ
60 70 80 90 100
DGAPDLYTYF TMLKSICVEV DHGLLPREEW QAKVAGNETS EAENDAAETE
110 120 130 140 150
EAEEDRISEE LDLEAQFHLH FCSLHHILTH LTRKAQEVTR KYQEMTGQVL
Length:150
Mass (Da):17,093
Last modified:November 1, 1997 - v1
Checksum:iC0CAACD39DBF8E31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95279 Genomic DNA. Translation: CAA64600.1.
BC009165 mRNA. Translation: AAH09165.1.
CCDSiCCDS21458.1.
RefSeqiNP_033407.1. NM_009381.3.
UniGeneiMm.28585.

Genome annotation databases

EnsembliENSMUST00000043077; ENSMUSP00000042988; ENSMUSG00000035686.
GeneIDi21835.
KEGGimmu:21835.
UCSCiuc009ije.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95279 Genomic DNA. Translation: CAA64600.1.
BC009165 mRNA. Translation: AAH09165.1.
CCDSiCCDS21458.1.
RefSeqiNP_033407.1. NM_009381.3.
UniGeneiMm.28585.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ONTX-ray2.65A1-150[»]
ProteinModelPortaliQ62264.
SMRiQ62264. Positions 8-149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59527N.
STRINGi10090.ENSMUSP00000042988.

PTM databases

iPTMnetiQ62264.
SwissPalmiQ62264.

Proteomic databases

EPDiQ62264.
MaxQBiQ62264.
PaxDbiQ62264.
PRIDEiQ62264.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043077; ENSMUSP00000042988; ENSMUSG00000035686.
GeneIDi21835.
KEGGimmu:21835.
UCSCiuc009ije.1. mouse.

Organism-specific databases

CTDi7069.
MGIiMGI:109126. Thrsp.

Phylogenomic databases

eggNOGiENOG410J34G. Eukaryota.
ENOG410YT7Q. LUCA.
GeneTreeiENSGT00500000044890.
HOGENOMiHOG000001157.
HOVERGENiHBG002528.
InParanoidiQ62264.
OMAiNMEQVVM.
OrthoDBiEOG7CZK87.
PhylomeDBiQ62264.
TreeFamiTF326826.

Miscellaneous databases

EvolutionaryTraceiQ62264.
NextBioi301290.
PROiQ62264.
SOURCEiSearch...

Gene expression databases

BgeeiQ62264.
ExpressionAtlasiQ62264. baseline and differential.
GenevisibleiQ62264. MM.

Family and domain databases

InterProiIPR009786. Spot_14.
[Graphical view]
PANTHERiPTHR14315. PTHR14315. 1 hit.
PfamiPF07084. Spot_14. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and initial characterization of human and mouse Spot 14 genes."
    Grillasca J.-P., Gastaldi M., Khiri H., Dace A., Peyrol N., Reynier P., Torresani J., Planells R.
    FEBS Lett. 401:38-42(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/cJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  3. Cited for: DISRUPTION PHENOTYPE.
  4. "The Spot 14 protein is required for de novo lipid synthesis in the lactating mammary gland."
    Zhu Q., Anderson G.W., Mucha G.T., Parks E.J., Metkowski J.K., Mariash C.N.
    Endocrinology 146:3343-3350(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.
  5. Cited for: DISRUPTION PHENOTYPE.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung and Pancreas.
  7. Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS), FUNCTION, INTERACTION WITH MID1IP1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTHRSP_MOUSE
AccessioniPrimary (citable) accession number: Q62264
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 16, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.