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Q62245 (SOS1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Son of sevenless homolog 1

Short name=SOS-1
Short name=mSOS-1
Gene names
Name:Sos1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1319 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes the exchange of Ras-bound GDP by GTP. Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity. Ref.2 Ref.3

Subunit structure

Interacts with GRB2. Forms a complex with phosphorylated MUC1 and GRB2 (via its SH3 domains). Interacts with phosphorylated LAT2. Interacts with NCK1 and NCK2 By similarity. Part of a complex consisting of ABI1, EPS8 and SOS1. Ref.2 Ref.3

Tissue specificity

Expressed in most embryonic and adult tissues.

Sequence similarities

Contains 1 DH (DBL-homology) domain.

Contains 1 N-terminal Ras-GEF domain.

Contains 1 PH domain.

Contains 1 Ras-GEF domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

P266632EBI-1693,EBI-6857429From a different organism.
GRB2P6299311EBI-1693,EBI-401755From a different organism.
Grb2Q606315EBI-1693,EBI-1688
sem-5P293552EBI-1693,EBI-315286From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13191319Son of sevenless homolog 1
PRO_0000068895

Regions

Domain200 – 390191DH
Domain444 – 548105PH
Domain597 – 741145N-terminal Ras-GEF
Domain780 – 1019240Ras-GEF
Compositional bias1244 – 12474Poly-Pro

Amino acid modifications

Modified residue10431Phosphoserine By similarity
Modified residue10781Phosphoserine Ref.5
Modified residue10821Phosphoserine Ref.5

Secondary structure

............................ 1319
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q62245 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 3286088A5BA0A4A6

FASTA1,319150,883
        10         20         30         40         50         60 
MQAQQLPYEF FSEENAPKWR GLLVPALKKV QGQVHPTLES NDDALQYVEE LILQLLNMLC 

        70         80         90        100        110        120 
QAQPRSASDV EERVQKSFPH PIDKWAIADA QSAIEKRKRR NPLSLPAERI HHLLREVLGY 

       130        140        150        160        170        180 
KIDHQVSVYI VAVLEYISAD ILKLVGNYVR NIRHYEITKQ DIKVAMCADK VLMDMFHQDV 

       190        200        210        220        230        240 
EDINILSLTD EEPSTSGEQT YYDLVKAFMA EIRQYIRELN LIIKVFREPF VSNSKLFSSN 

       250        260        270        280        290        300 
DVENIFSRIV DIHELSVKLL GHIEDTVEMT DEGSPHPLVG SCFEDLAEEL AFDPYESYAR 

       310        320        330        340        350        360 
DILRPGFHGH FLSQLSKPGA ALYLQSIGEG FKEAVQYVLP RLLLAPVYHC LHYFELLKQL 

       370        380        390        400        410        420 
EEKSEDQEDK ECMKQAITAL LNVQSGMEKI CSKSLAKRRL SESACRFYSQ QMKGKQLAIK 

       430        440        450        460        470        480 
KMNEIQKNID GWEGKDIGQC CNEFIMEGTL TRVGAKHERH IFLFDGLMIC CKSNHGQPRL 

       490        500        510        520        530        540 
PGASSAEYRL KEKFFMRKVQ INDKDDTSEY KHAFEIILKD GNSVIFSAKS AEEKNNWMAA 

       550        560        570        580        590        600 
LISLQYRSTL ERMLDVTVLQ EEKEEQMRLP SAEVYRFAEP DSEENILFEE NVQPKAGIPI 

       610        620        630        640        650        660 
IKAGTVLKLI ERLTYHMYAD PNFVRTFLTT YRSFCRPQEL LSLLIERFEI PEPEPTEADR 

       670        680        690        700        710        720 
IAIENGDQPL SAELKRFRKE YIQPVQLRVL NVCRHWVEHH FYDFERDADL LQRMEEFIGT 

       730        740        750        760        770        780 
VRGKAMKKWV ESITKIIQRK KIARDNGPGH NITFQSSPPT VEWHISRPGH IETFDLLTLH 

       790        800        810        820        830        840 
PIEIARQLTL LESDLYRAVQ PSELVGSVWT KEDKEINSPN LLKMIRHTTN LTLWFEKCIV 

       850        860        870        880        890        900 
ETENLEERVA VVSRIIEILQ VFQELNNFNG VLEVVSAMNS SPVYRLDHTF EQIPSRQKKI 

       910        920        930        940        950        960 
LEEAHELSED HYKKYLAKLR SINPPCVPFF GIYLTNILKT EEGNPEVLRR HGKELINFSK 

       970        980        990       1000       1010       1020 
RRRVAEITGE IQQYQNQPYC LRVEPDIKRF FENLNPMGNS MEKEFTDYLF NKSLEIEPRH 

      1030       1040       1050       1060       1070       1080 
PKPLPRFPKK YSYPLKSPGV RPSNPRPGTM RHPTPLQQEP RKISYSRIPE SETESTASAP 

      1090       1100       1110       1120       1130       1140 
NSPRTPLTPP PASGTSSNTD VCSVFDSDHS ASPFHSRSAS VSSISLSKGT DEVPVPPPVP 

      1150       1160       1170       1180       1190       1200 
PRRRPESAPA ESSPSKIMSK HLDSPPAIPP RQPTSKAYSP RYSISDRTSI SDPPESPPLL 

      1210       1220       1230       1240       1250       1260 
PPREPVRTPD VFSSSPLHLQ PPPLGKKSDH GNAFFPNSPS PFTPPPPQTP SPHGTRRHLP 

      1270       1280       1290       1300       1310 
SPPLTQEMDL HSIAGPPVPP RQSTSQLIPK LPPKTYKREH THPSMHRDGP PLLENAHSS 

« Hide

References

« Hide 'large scale' references
[1]"Identification of murine homologues of the Drosophila son of sevenless gene: potential activators of ras."
Bowtell D.D., Fu P., Simon M.A., Senior P.V.
Proc. Natl. Acad. Sci. U.S.A. 89:6511-6515(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss.
Tissue: Eye.
[2]"EPS8 and E3B1 transduce signals from Ras to Rac."
Scita G., Nordstrom J., Carbone R., Tenca P., Giardina G., Gutkind S., Bjarnegard M., Betsholtz C., Di Fiore P.P.
Nature 401:290-293(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH ABI1 AND EPS8E.
[3]"An effector region in Eps8 is responsible for the activation of the Rac-specific GEF activity of Sos-1 and for the proper localization of the Rac-based actin-polymerizing machine."
Scita G., Tenca P., Areces L.B., Tocchetti A., Frittoli E., Giardina G., Ponzanelli I., Sini P., Innocenti M., Di Fiore P.P.
J. Cell Biol. 154:1031-1044(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH EPS8.
[4]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain cortex.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1078 AND SER-1082, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"The solution structure of the pleckstrin homology domain of mouse Son-of-sevenless 1 (mSos1)."
Koshiba S., Kigawa T., Kim J.-H., Shirouzu M., Bowtell D., Yokoyama S.
J. Mol. Biol. 269:579-591(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 415-548.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11574 mRNA. Translation: CAA77662.1.
Z11578 mRNA. Translation: CAA77665.1.
PIRS25715.
S25716.
RefSeqNP_033257.2. NM_009231.2.
UniGeneMm.360004.
Mm.60975.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GBQNMR-B1135-1144[»]
1PMSNMR-A415-548[»]
2GBQNMR-B1135-1144[»]
3GBQNMR-B1135-1144[»]
4GBQNMR-B1135-1144[»]
ProteinModelPortalQ62245.
SMRQ62245. Positions 6-183, 198-1046.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203394. 6 interactions.
DIPDIP-29230N.
IntActQ62245. 17 interactions.
MINTMINT-158444.

PTM databases

PhosphoSiteQ62245.

Proteomic databases

PaxDbQ62245.
PRIDEQ62245.

Protocols and materials databases

DNASU20662.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000068714; ENSMUSP00000067786; ENSMUSG00000024241.
GeneID20662.
KEGGmmu:20662.
UCSCuc008drg.1. mouse.

Organism-specific databases

CTD6654.
MGIMGI:98354. Sos1.

Phylogenomic databases

eggNOGNOG265981.
GeneTreeENSGT00730000110221.
HOGENOMHOG000013040.
HOVERGENHBG017831.
InParanoidQ62245.
KOK03099.
OMASVFDSDH.
OrthoDBEOG7XSTD0.
PhylomeDBQ62245.
TreeFamTF317296.

Gene expression databases

ArrayExpressQ62245.
BgeeQ62245.
CleanExMM_SOS1.
GenevestigatorQ62245.

Family and domain databases

Gene3D1.10.20.10. 1 hit.
1.10.840.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR000219. DH-domain.
IPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR008937. Ras_GEF.
IPR023578. Ras_GEF_dom.
IPR001895. RasGRF_CDC25.
[Graphical view]
PANTHERPTHR23113. PTHR23113. 1 hit.
PfamPF00125. Histone. 1 hit.
PF00169. PH. 1 hit.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMSSF47113. SSF47113. 1 hit.
SSF48065. SSF48065. 1 hit.
SSF48366. SSF48366. 1 hit.
PROSITEPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSOS1. mouse.
EvolutionaryTraceQ62245.
NextBio299109.
PROQ62245.
SOURCESearch...

Entry information

Entry nameSOS1_MOUSE
AccessionPrimary (citable) accession number: Q62245
Secondary accession number(s): Q62244
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 15, 1999
Last modified: April 16, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot