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Q62245

- SOS1_MOUSE

UniProt

Q62245 - SOS1_MOUSE

Protein

Son of sevenless homolog 1

Gene

Sos1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Promotes the exchange of Ras-bound GDP by GTP. Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity.2 Publications

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. protein binding Source: UniProtKB
    3. Ras guanyl-nucleotide exchange factor activity Source: MGI
    4. Rho guanyl-nucleotide exchange factor activity Source: InterPro

    GO - Biological processi

    1. neurotrophin TRK receptor signaling pathway Source: Ensembl
    2. positive regulation of epidermal growth factor receptor signaling pathway Source: Ensembl
    3. positive regulation of Ras GTPase activity Source: GOC
    4. positive regulation of small GTPase mediated signal transduction Source: Ensembl
    5. Ras protein signal transduction Source: Ensembl
    6. small GTPase mediated signal transduction Source: MGI

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Enzyme and pathway databases

    ReactomeiREACT_188185. DAP12 signaling.
    REACT_188194. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
    REACT_188202. FCERI mediated Ca+2 mobilization.
    REACT_188528. GRB2 events in ERBB2 signaling.
    REACT_188530. FCERI mediated MAPK activation.
    REACT_188574. SHC1 events in ERBB2 signaling.
    REACT_188578. Signaling by SCF-KIT.
    REACT_188580. SHC1 events in ERBB4 signaling.
    REACT_196455. Signaling by FGFR mutants.
    REACT_198350. EGFR Transactivation by Gastrin.
    REACT_199123. Signaling by constitutively active EGFR.
    REACT_204733. G alpha (12/13) signalling events.
    REACT_209641. NRAGE signals death through JNK.
    REACT_210090. Rho GTPase cycle.
    REACT_210793. Interleukin receptor SHC signaling.
    REACT_211860. Tie2 Signaling.
    REACT_213364. Role of LAT2/NTAL/LAB on calcium mobilization.
    REACT_227425. Regulation of KIT signaling.
    REACT_94437. SHC-mediated cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Son of sevenless homolog 1
    Short name:
    SOS-1
    Short name:
    mSOS-1
    Gene namesi
    Name:Sos1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:98354. Sos1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. neuronal cell body Source: Ensembl
    3. plasma membrane Source: Ensembl
    4. postsynaptic density Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13191319Son of sevenless homolog 1PRO_0000068895Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1043 – 10431PhosphoserineBy similarity
    Modified residuei1078 – 10781Phosphoserine1 Publication
    Modified residuei1082 – 10821Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ62245.
    PaxDbiQ62245.
    PRIDEiQ62245.

    PTM databases

    PhosphoSiteiQ62245.

    Expressioni

    Tissue specificityi

    Expressed in most embryonic and adult tissues.

    Gene expression databases

    ArrayExpressiQ62245.
    BgeeiQ62245.
    CleanExiMM_SOS1.
    GenevestigatoriQ62245.

    Interactioni

    Subunit structurei

    Interacts with GRB2. Forms a complex with phosphorylated MUC1 and GRB2 (via its SH3 domains). Interacts with phosphorylated LAT2. Interacts with NCK1 and NCK2 By similarity. Part of a complex consisting of ABI1, EPS8 and SOS1.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    P266632EBI-1693,EBI-6857429From a different organism.
    GRB2P6299311EBI-1693,EBI-401755From a different organism.
    Grb2Q606317EBI-1693,EBI-1688
    sem-5P293552EBI-1693,EBI-315286From a different organism.

    Protein-protein interaction databases

    BioGridi203394. 6 interactions.
    DIPiDIP-29230N.
    IntActiQ62245. 17 interactions.
    MINTiMINT-158444.

    Structurei

    Secondary structure

    1
    1319
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni416 – 4183
    Helixi419 – 4279
    Beta strandi437 – 4415
    Turni443 – 4453
    Beta strandi446 – 4549
    Beta strandi459 – 4635
    Beta strandi465 – 4717
    Beta strandi481 – 4844
    Beta strandi490 – 4956
    Beta strandi500 – 5023
    Beta strandi514 – 5174
    Beta strandi519 – 5213
    Beta strandi523 – 5275
    Beta strandi529 – 5313
    Helixi532 – 54514

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B07X-ray2.50C1135-1144[»]
    1GBQNMR-B1135-1144[»]
    1PMSNMR-A414-548[»]
    2GBQNMR-B1135-1144[»]
    3GBQNMR-B1135-1144[»]
    4GBQNMR-B1135-1144[»]
    ProteinModelPortaliQ62245.
    SMRiQ62245. Positions 6-183, 198-1046.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ62245.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini200 – 390191DHPROSITE-ProRule annotationAdd
    BLAST
    Domaini444 – 548105PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini597 – 741145N-terminal Ras-GEFPROSITE-ProRule annotationAdd
    BLAST
    Domaini780 – 1019240Ras-GEFPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1244 – 12474Poly-Pro

    Sequence similaritiesi

    Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
    Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG265981.
    GeneTreeiENSGT00730000110221.
    HOGENOMiHOG000013040.
    HOVERGENiHBG017831.
    InParanoidiQ62245.
    KOiK03099.
    OMAiSVFDSDH.
    OrthoDBiEOG7XSTD0.
    PhylomeDBiQ62245.
    TreeFamiTF317296.

    Family and domain databases

    Gene3Di1.10.20.10. 1 hit.
    1.10.840.10. 1 hit.
    1.20.900.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR000219. DH-domain.
    IPR009072. Histone-fold.
    IPR007125. Histone_core_D.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000651. Ras-like_Gua-exchang_fac_N.
    IPR019804. Ras_G-nucl-exch_fac_CS.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    [Graphical view]
    PfamiPF00125. Histone. 1 hit.
    PF00169. PH. 1 hit.
    PF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    PF00621. RhoGEF. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    SM00325. RhoGEF. 1 hit.
    [Graphical view]
    SUPFAMiSSF47113. SSF47113. 1 hit.
    SSF48065. SSF48065. 1 hit.
    SSF48366. SSF48366. 1 hit.
    PROSITEiPS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00720. RASGEF. 1 hit.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q62245-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAQQLPYEF FSEENAPKWR GLLVPALKKV QGQVHPTLES NDDALQYVEE     50
    LILQLLNMLC QAQPRSASDV EERVQKSFPH PIDKWAIADA QSAIEKRKRR 100
    NPLSLPAERI HHLLREVLGY KIDHQVSVYI VAVLEYISAD ILKLVGNYVR 150
    NIRHYEITKQ DIKVAMCADK VLMDMFHQDV EDINILSLTD EEPSTSGEQT 200
    YYDLVKAFMA EIRQYIRELN LIIKVFREPF VSNSKLFSSN DVENIFSRIV 250
    DIHELSVKLL GHIEDTVEMT DEGSPHPLVG SCFEDLAEEL AFDPYESYAR 300
    DILRPGFHGH FLSQLSKPGA ALYLQSIGEG FKEAVQYVLP RLLLAPVYHC 350
    LHYFELLKQL EEKSEDQEDK ECMKQAITAL LNVQSGMEKI CSKSLAKRRL 400
    SESACRFYSQ QMKGKQLAIK KMNEIQKNID GWEGKDIGQC CNEFIMEGTL 450
    TRVGAKHERH IFLFDGLMIC CKSNHGQPRL PGASSAEYRL KEKFFMRKVQ 500
    INDKDDTSEY KHAFEIILKD GNSVIFSAKS AEEKNNWMAA LISLQYRSTL 550
    ERMLDVTVLQ EEKEEQMRLP SAEVYRFAEP DSEENILFEE NVQPKAGIPI 600
    IKAGTVLKLI ERLTYHMYAD PNFVRTFLTT YRSFCRPQEL LSLLIERFEI 650
    PEPEPTEADR IAIENGDQPL SAELKRFRKE YIQPVQLRVL NVCRHWVEHH 700
    FYDFERDADL LQRMEEFIGT VRGKAMKKWV ESITKIIQRK KIARDNGPGH 750
    NITFQSSPPT VEWHISRPGH IETFDLLTLH PIEIARQLTL LESDLYRAVQ 800
    PSELVGSVWT KEDKEINSPN LLKMIRHTTN LTLWFEKCIV ETENLEERVA 850
    VVSRIIEILQ VFQELNNFNG VLEVVSAMNS SPVYRLDHTF EQIPSRQKKI 900
    LEEAHELSED HYKKYLAKLR SINPPCVPFF GIYLTNILKT EEGNPEVLRR 950
    HGKELINFSK RRRVAEITGE IQQYQNQPYC LRVEPDIKRF FENLNPMGNS 1000
    MEKEFTDYLF NKSLEIEPRH PKPLPRFPKK YSYPLKSPGV RPSNPRPGTM 1050
    RHPTPLQQEP RKISYSRIPE SETESTASAP NSPRTPLTPP PASGTSSNTD 1100
    VCSVFDSDHS ASPFHSRSAS VSSISLSKGT DEVPVPPPVP PRRRPESAPA 1150
    ESSPSKIMSK HLDSPPAIPP RQPTSKAYSP RYSISDRTSI SDPPESPPLL 1200
    PPREPVRTPD VFSSSPLHLQ PPPLGKKSDH GNAFFPNSPS PFTPPPPQTP 1250
    SPHGTRRHLP SPPLTQEMDL HSIAGPPVPP RQSTSQLIPK LPPKTYKREH 1300
    THPSMHRDGP PLLENAHSS 1319
    Length:1,319
    Mass (Da):150,883
    Last modified:July 15, 1999 - v2
    Checksum:i3286088A5BA0A4A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11574 mRNA. Translation: CAA77662.1.
    Z11578 mRNA. Translation: CAA77665.1.
    CCDSiCCDS37704.1.
    PIRiS25715.
    S25716.
    RefSeqiNP_033257.2. NM_009231.2.
    UniGeneiMm.360004.
    Mm.60975.

    Genome annotation databases

    EnsembliENSMUST00000068714; ENSMUSP00000067786; ENSMUSG00000024241.
    GeneIDi20662.
    KEGGimmu:20662.
    UCSCiuc008drg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11574 mRNA. Translation: CAA77662.1 .
    Z11578 mRNA. Translation: CAA77665.1 .
    CCDSi CCDS37704.1.
    PIRi S25715.
    S25716.
    RefSeqi NP_033257.2. NM_009231.2.
    UniGenei Mm.360004.
    Mm.60975.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B07 X-ray 2.50 C 1135-1144 [» ]
    1GBQ NMR - B 1135-1144 [» ]
    1PMS NMR - A 414-548 [» ]
    2GBQ NMR - B 1135-1144 [» ]
    3GBQ NMR - B 1135-1144 [» ]
    4GBQ NMR - B 1135-1144 [» ]
    ProteinModelPortali Q62245.
    SMRi Q62245. Positions 6-183, 198-1046.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203394. 6 interactions.
    DIPi DIP-29230N.
    IntActi Q62245. 17 interactions.
    MINTi MINT-158444.

    PTM databases

    PhosphoSitei Q62245.

    Proteomic databases

    MaxQBi Q62245.
    PaxDbi Q62245.
    PRIDEi Q62245.

    Protocols and materials databases

    DNASUi 20662.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000068714 ; ENSMUSP00000067786 ; ENSMUSG00000024241 .
    GeneIDi 20662.
    KEGGi mmu:20662.
    UCSCi uc008drg.1. mouse.

    Organism-specific databases

    CTDi 6654.
    MGIi MGI:98354. Sos1.

    Phylogenomic databases

    eggNOGi NOG265981.
    GeneTreei ENSGT00730000110221.
    HOGENOMi HOG000013040.
    HOVERGENi HBG017831.
    InParanoidi Q62245.
    KOi K03099.
    OMAi SVFDSDH.
    OrthoDBi EOG7XSTD0.
    PhylomeDBi Q62245.
    TreeFami TF317296.

    Enzyme and pathway databases

    Reactomei REACT_188185. DAP12 signaling.
    REACT_188194. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
    REACT_188202. FCERI mediated Ca+2 mobilization.
    REACT_188528. GRB2 events in ERBB2 signaling.
    REACT_188530. FCERI mediated MAPK activation.
    REACT_188574. SHC1 events in ERBB2 signaling.
    REACT_188578. Signaling by SCF-KIT.
    REACT_188580. SHC1 events in ERBB4 signaling.
    REACT_196455. Signaling by FGFR mutants.
    REACT_198350. EGFR Transactivation by Gastrin.
    REACT_199123. Signaling by constitutively active EGFR.
    REACT_204733. G alpha (12/13) signalling events.
    REACT_209641. NRAGE signals death through JNK.
    REACT_210090. Rho GTPase cycle.
    REACT_210793. Interleukin receptor SHC signaling.
    REACT_211860. Tie2 Signaling.
    REACT_213364. Role of LAT2/NTAL/LAB on calcium mobilization.
    REACT_227425. Regulation of KIT signaling.
    REACT_94437. SHC-mediated cascade.

    Miscellaneous databases

    ChiTaRSi SOS1. mouse.
    EvolutionaryTracei Q62245.
    NextBioi 299109.
    PROi Q62245.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q62245.
    Bgeei Q62245.
    CleanExi MM_SOS1.
    Genevestigatori Q62245.

    Family and domain databases

    Gene3Di 1.10.20.10. 1 hit.
    1.10.840.10. 1 hit.
    1.20.900.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR000219. DH-domain.
    IPR009072. Histone-fold.
    IPR007125. Histone_core_D.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000651. Ras-like_Gua-exchang_fac_N.
    IPR019804. Ras_G-nucl-exch_fac_CS.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    [Graphical view ]
    Pfami PF00125. Histone. 1 hit.
    PF00169. PH. 1 hit.
    PF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    PF00621. RhoGEF. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    SM00325. RhoGEF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47113. SSF47113. 1 hit.
    SSF48065. SSF48065. 1 hit.
    SSF48366. SSF48366. 1 hit.
    PROSITEi PS50010. DH_2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS00720. RASGEF. 1 hit.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of murine homologues of the Drosophila son of sevenless gene: potential activators of ras."
      Bowtell D.D., Fu P., Simon M.A., Senior P.V.
      Proc. Natl. Acad. Sci. U.S.A. 89:6511-6515(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss.
      Tissue: Eye.
    2. Cited for: FUNCTION, IDENTIFICATION IN A COMPLEX WITH ABI1 AND EPS8E.
    3. "An effector region in Eps8 is responsible for the activation of the Rac-specific GEF activity of Sos-1 and for the proper localization of the Rac-based actin-polymerizing machine."
      Scita G., Tenca P., Areces L.B., Tocchetti A., Frittoli E., Giardina G., Ponzanelli I., Sini P., Innocenti M., Di Fiore P.P.
      J. Cell Biol. 154:1031-1044(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH EPS8.
    4. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1078 AND SER-1082, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The solution structure of the pleckstrin homology domain of mouse Son-of-sevenless 1 (mSos1)."
      Koshiba S., Kigawa T., Kim J.-H., Shirouzu M., Bowtell D., Yokoyama S.
      J. Mol. Biol. 269:579-591(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 415-548.

    Entry informationi

    Entry nameiSOS1_MOUSE
    AccessioniPrimary (citable) accession number: Q62245
    Secondary accession number(s): Q62244
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3