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Protein

Myomesin-1

Gene

Myom1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May link the intermediate filament cytoskeleton to the M-disk of the myofibrils in striated muscle. May also contact myosin filaments. Also binds beta-integrins.

GO - Molecular functioni

  • identical protein binding Source: MGI
  • protein homodimerization activity Source: UniProtKB
  • structural constituent of cytoskeleton Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Names & Taxonomyi

Protein namesi
Recommended name:
Myomesin-1
Alternative name(s):
Myomesin family member 1
Skelemin
Gene namesi
Name:Myom1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1341430. Myom1.

Subcellular locationi

GO - Cellular componenti

  • cytoskeleton Source: MGI
  • M band Source: UniProtKB
  • myosin filament Source: UniProtKB-KW
  • sarcomere Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16671667Myomesin-1PRO_0000072685Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei124 – 1241PhosphoserineCombined sources
Modified residuei142 – 1421PhosphoserineCombined sources
Modified residuei863 – 8631PhosphoserineCombined sources
Modified residuei867 – 8671PhosphoserineCombined sources
Modified residuei1036 – 10361PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ62234.
PaxDbiQ62234.
PeptideAtlasiQ62234.
PRIDEiQ62234.

2D gel databases

SWISS-2DPAGEQ62234.

PTM databases

iPTMnetiQ62234.
PhosphoSiteiQ62234.

Expressioni

Tissue specificityi

Ubiquitously expressed in all striated muscles. Expressed in all fiber types.1 Publication

Gene expression databases

BgeeiQ62234.
CleanExiMM_MYOM1.
ExpressionAtlasiQ62234. baseline and differential.
GenevisibleiQ62234. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with TTN/titin and PNKD (By similarity).By similarity

GO - Molecular functioni

  • identical protein binding Source: MGI
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi201675. 1 interaction.
IntActiQ62234. 1 interaction.
MINTiMINT-4102904.
STRINGi10090.ENSMUSP00000072945.

Structurei

Secondary structure

1
1667
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1212 – 12165Combined sources
Beta strandi1227 – 12293Combined sources
Beta strandi1231 – 12377Combined sources
Turni1238 – 12403Combined sources
Beta strandi1241 – 12477Combined sources
Beta strandi1252 – 12543Combined sources
Beta strandi1256 – 12616Combined sources
Beta strandi1268 – 12714Combined sources
Beta strandi1274 – 12763Combined sources
Beta strandi1278 – 12803Combined sources
Beta strandi1283 – 12875Combined sources
Turni1292 – 12943Combined sources
Beta strandi1297 – 13048Combined sources
Beta strandi1307 – 13148Combined sources
Helixi1317 – 132711Combined sources
Helixi1333 – 13353Combined sources
Beta strandi1356 – 13594Combined sources
Beta strandi1373 – 13753Combined sources
Beta strandi1385 – 13873Combined sources
Beta strandi1394 – 13974Combined sources
Turni1402 – 14043Combined sources
Beta strandi1406 – 14116Combined sources
Beta strandi1414 – 14174Combined sources
Beta strandi1423 – 14253Combined sources
Helixi1428 – 14314Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JTDNMR-A1208-1329[»]
4V10NMR-A1208-1434[»]
ProteinModelPortaliQ62234.
SMRiQ62234. Positions 1123-1648.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62234.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini258 – 34992Ig-like C2-type 1Add
BLAST
Domaini376 – 478103Ig-like C2-type 2Add
BLAST
Domaini492 – 58796Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini620 – 71495Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini721 – 81494Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini918 – 101699Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST
Domaini1023 – 1122100Fibronectin type-III 5PROSITE-ProRule annotationAdd
BLAST
Domaini1114 – 121299Ig-like C2-type 3Add
BLAST
Domaini1340 – 142687Ig-like C2-type 4Add
BLAST
Domaini1555 – 164490Ig-like C2-type 5Add
BLAST

Sequence similaritiesi

Contains 5 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat

Phylogenomic databases

eggNOGiENOG410IJ5G. Eukaryota.
ENOG410ZSRR. LUCA.
GeneTreeiENSGT00840000129736.
HOGENOMiHOG000293283.
HOVERGENiHBG004977.
InParanoidiQ62234.
OMAiEDTAQYR.
OrthoDBiEOG7MSMN2.
PhylomeDBiQ62234.
TreeFamiTF331825.

Family and domain databases

Gene3Di2.60.40.10. 12 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF00041. fn3. 5 hits.
PF07679. I-set. 5 hits.
[Graphical view]
SMARTiSM00060. FN3. 5 hits.
SM00409. IG. 7 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 7 hits.
SSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 5 hits.
PS50835. IG_LIKE. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q62234-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLPFYQRSH QHYDLSYRNK DLRTTMSHYQ QEKKRSAVYT HGSTAYSSRS
60 70 80 90 100
LAARRQESEA FSQASATSYQ QQASQTYSLG ASSSSRHSQG SEVSRKTASA
110 120 130 140 150
YDYGYSHGLT DSSLLLEDYS SKLSPQTKRA KRSLLSGEET GSLPGNYLVP
160 170 180 190 200
IYSGRQVHIS GIRDSEEERI KEAAAYIAQK TLLASEEAIA ASKQSTASKQ
210 220 230 240 250
SATSKRTTST LQREETFEKK SRNIAIREKA EELSLKKTLE ETQTYHGKLN
260 270 280 290 300
EDHLLHAPEF IIKPRSHTVW EKENVKLHCS VAGWPEPRLT WYKNQVPINV
310 320 330 340 350
HANPGKYIIE SRYGMHTLEI SKCDFEDTAQ YRASAMNVQG ELSAYASVVV
360 370 380 390 400
KRYKGELDES LLRGGVSMPL SFAVTPYGYA SKFEIHFDDK FDVSFGREGE
410 420 430 440 450
TMSLGCRVVI TPEIKHFQPE VQWYRNGAPV SPSKWVQPHW SGDRATLTFS
460 470 480 490 500
HLNKEDEGLY TIRVRMGEYY EQYSAYVFVR DADAEIEGAP AAPLDVVSLD
510 520 530 540 550
ANKDYIIISW KQPAVDGGSP ILGYFIDKCE VGTDTWSQCN DTPVKFARFP
560 570 580 590 600
VTGLIEGRSY IFRVRAVNKT GIGLPSRVSE PVAALDPAEK ARLKSHPSAP
610 620 630 640 650
WTGQIIVTEE EPTEGVIPGP PTDLSVTEAT RSYVVLSWKP PGQRGHEGIM
660 670 680 690 700
YFVEKCDVGA ENWQRVNTEL PVKSPRFALF DLVEGKSYRF RVRCSNSAGV
710 720 730 740 750
GEPSETTEVT VVGDKLDIPK APGKIIPSRN TDTSVVVSWE ESRDAKELVG
760 770 780 790 800
YYIEASVVGS GKWEPCNNNP VKGSRFTCHG LTTAQSYIFR VRAVNAAGLS
810 820 830 840 850
EYSQDSEAIE VKAAIGGGVS PDVWPQLSDT PGGLTDSRGG MNGASPPTSQ
860 870 880 890 900
KDALLGSNPN KPSPPSSPSS RGQKEVSTVS ESVQEPLSSP PQEAAPEEEQ
910 920 930 940 950
SQSEPPKKKK DPVAVPSAPY DITCLESFRD SMVLGWKQPD TTGGAEITGY
960 970 980 990 1000
YVNYREVVGE VPGKWREANI KAVSDAAYKI SNLKENTLYQ FQVSAMNIAG
1010 1020 1030 1040 1050
LGAPSTVSEC FKCEEWTIAV PGPPHSVKLS EVRKNSLVLQ WKPPVYSGRT
1060 1070 1080 1090 1100
PVTGYFVDLK EASAKDDQWR GLNEAAIVNK YLRVQGLKEG TSYVFRVRAV
1110 1120 1130 1140 1150
NQAGVGKPSD LAGPVVAETR PGTKEVVVSV DDDGVISLNF ECDQMTPKSE
1160 1170 1180 1190 1200
FVWSKDYVPT EDSPRLEVEN KGDKTKMTFK DLGTDDLGTY SCDVTDTDGI
1210 1220 1230 1240 1250
ASSYLIDEEE MKRLLALSQE HKFPTVPTKS ELAVEILEKG QVRFWMQAEK
1260 1270 1280 1290 1300
LSSNAKVSYI FNEKEIFEGP KYKMHIDRNT GIIEMFMEKL QDEDEGTYTF
1310 1320 1330 1340 1350
QIQDGKATGH STLVLIGDVY KKLQKEAEFQ RQEWIRKQGP HFAEYLSWEV
1360 1370 1380 1390 1400
TGECNVLLKC KVANIKKETH IVWYKDEREI SVDEKHDFKD GICTLLITEF
1410 1420 1430 1440 1450
SKKDAGFYEV ILKDDRGKDK SRLKLVDEAF QDLMTEVCKK IALSATDLKI
1460 1470 1480 1490 1500
QSTAEGIRLY SFVCYYLDDL KVNWSHNGTG IKYTDRVKSG VTGEQIWLQI
1510 1520 1530 1540 1550
NEPTPNDKGK YVMELFDGKT GHQKTVDLSG QAFDEAFAEF QRLKQAAIAE
1560 1570 1580 1590 1600
KNRARVLGGL PDVVTIQEGK ALNLTCNVWG DPPPEVSWLK NEKPLTSDDH
1610 1620 1630 1640 1650
CSLKFEAGKT AFFTISGVST ADSGKYGLVV KNKYGSETSD FTVSVFIPEE
1660
ELRKGAMEPP KGNQKSK
Length:1,667
Mass (Da):185,464
Last modified:November 4, 2008 - v2
Checksum:iCDFDE2DE4A54DB42
GO
Isoform 2 (identifier: Q62234-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     816-913: Missing.

Show »
Length:1,569
Mass (Da):175,451
Checksum:i59EEA98261A9E2E2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1342SL → V in CAA80488 (PubMed:8408035).Curated
Sequence conflicti133 – 1342SL → V in CAB46494 (PubMed:10036188).Curated
Sequence conflicti383 – 3831F → V in CAA80488 (PubMed:8408035).Curated
Sequence conflicti383 – 3831F → V in CAB46494 (PubMed:10036188).Curated
Sequence conflicti1092 – 10921S → C in CAA80488 (PubMed:8408035).Curated
Sequence conflicti1092 – 10921S → C in CAB46494 (PubMed:10036188).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei816 – 91398Missing in isoform 2. 1 PublicationVSP_035664Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22866 mRNA. Translation: CAA80488.1.
AJ012072 mRNA. Translation: CAB46494.1.
AC154187 Genomic DNA. No translation available.
AC154218 Genomic DNA. No translation available.
AC154717 Genomic DNA. No translation available.
BC060378 mRNA. Translation: AAH60378.1.
CCDSiCCDS37685.1. [Q62234-1]
CCDS50176.1. [Q62234-2]
PIRiA48594.
RefSeqiNP_001077403.1. NM_001083934.1. [Q62234-2]
NP_034997.2. NM_010867.2. [Q62234-1]
UniGeneiMm.4103.

Genome annotation databases

EnsembliENSMUST00000073211; ENSMUSP00000072945; ENSMUSG00000024049. [Q62234-1]
ENSMUST00000179759; ENSMUSP00000136266; ENSMUSG00000024049. [Q62234-2]
GeneIDi17929.
KEGGimmu:17929.
UCSCiuc008dlx.1. mouse. [Q62234-1]
uc008dly.1. mouse. [Q62234-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z22866 mRNA. Translation: CAA80488.1.
AJ012072 mRNA. Translation: CAB46494.1.
AC154187 Genomic DNA. No translation available.
AC154218 Genomic DNA. No translation available.
AC154717 Genomic DNA. No translation available.
BC060378 mRNA. Translation: AAH60378.1.
CCDSiCCDS37685.1. [Q62234-1]
CCDS50176.1. [Q62234-2]
PIRiA48594.
RefSeqiNP_001077403.1. NM_001083934.1. [Q62234-2]
NP_034997.2. NM_010867.2. [Q62234-1]
UniGeneiMm.4103.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JTDNMR-A1208-1329[»]
4V10NMR-A1208-1434[»]
ProteinModelPortaliQ62234.
SMRiQ62234. Positions 1123-1648.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201675. 1 interaction.
IntActiQ62234. 1 interaction.
MINTiMINT-4102904.
STRINGi10090.ENSMUSP00000072945.

PTM databases

iPTMnetiQ62234.
PhosphoSiteiQ62234.

2D gel databases

SWISS-2DPAGEQ62234.

Proteomic databases

MaxQBiQ62234.
PaxDbiQ62234.
PeptideAtlasiQ62234.
PRIDEiQ62234.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073211; ENSMUSP00000072945; ENSMUSG00000024049. [Q62234-1]
ENSMUST00000179759; ENSMUSP00000136266; ENSMUSG00000024049. [Q62234-2]
GeneIDi17929.
KEGGimmu:17929.
UCSCiuc008dlx.1. mouse. [Q62234-1]
uc008dly.1. mouse. [Q62234-2]

Organism-specific databases

CTDi8736.
MGIiMGI:1341430. Myom1.

Phylogenomic databases

eggNOGiENOG410IJ5G. Eukaryota.
ENOG410ZSRR. LUCA.
GeneTreeiENSGT00840000129736.
HOGENOMiHOG000293283.
HOVERGENiHBG004977.
InParanoidiQ62234.
OMAiEDTAQYR.
OrthoDBiEOG7MSMN2.
PhylomeDBiQ62234.
TreeFamiTF331825.

Miscellaneous databases

ChiTaRSiMyom1. mouse.
EvolutionaryTraceiQ62234.
PROiQ62234.
SOURCEiSearch...

Gene expression databases

BgeeiQ62234.
CleanExiMM_MYOM1.
ExpressionAtlasiQ62234. baseline and differential.
GenevisibleiQ62234. MM.

Family and domain databases

Gene3Di2.60.40.10. 12 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
[Graphical view]
PfamiPF00041. fn3. 5 hits.
PF07679. I-set. 5 hits.
[Graphical view]
SMARTiSM00060. FN3. 5 hits.
SM00409. IG. 7 hits.
SM00408. IGc2. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 7 hits.
SSF49265. SSF49265. 3 hits.
PROSITEiPS50853. FN3. 5 hits.
PS50835. IG_LIKE. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Skelemin, a cytoskeletal M-disc periphery protein, contains motifs of adhesion/recognition and intermediate filament proteins."
    Price M.G., Gomer R.H.
    J. Biol. Chem. 268:21800-21810(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/10J.
    Tissue: Muscle.
  2. "M band proteins myomesin and skelemin are encoded by the same gene: analysis of its organization and expression."
    Steiner F., Weber K., Furst D.O.
    Genomics 56:78-89(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: NMRI.
    Tissue: Mammary tumor.
  5. "Identification of an interaction between the M-band protein skelemin and beta-integrin subunits. Colocalization of a skelemin-like protein with beta1- and beta3-integrins in non-muscle cells."
    Reddy K.B., Gascard P., Price M.G., Negrescu E.V., Fox J.E.B.
    J. Biol. Chem. 273:35039-35047(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BETA-INTEGRIN.
  6. "Myomesin 3, a novel structural component of the M-band in striated muscle."
    Schoenauer R., Lange S., Hirschy A., Ehler E., Perriard J.C., Agarkova I.
    J. Mol. Biol. 376:338-351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124; SER-142; SER-863; SER-867 AND SER-1036, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart and Lung.
  8. "Sarcosin (Krp1) in skeletal muscle differentiation: gene expression profiling and knockdown experiments."
    du Puy L., Beqqali A., van Tol H.T., Monshouwer-Kloots J., Passier R., Haagsman H.P., Roelen B.A.
    Int. J. Dev. Biol. 56:301-309(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMYOM1_MOUSE
AccessioniPrimary (citable) accession number: Q62234
Secondary accession number(s): Q546T8, Q6PAC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 4, 2008
Last modified: July 6, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.