Reviewed, UniProtKB/Swiss-Prot Q62233 (SIX3_MOUSE)

Names and origin

Protein names

Recommended name:
    Homeobox protein SIX3
Alternative name(s):
    Sine oculis homeobox homolog 3

Gene names

Name:

Six3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in visual system development.
Subcellular location	Nucleus.
Developmental stage	First expressed at E6.5 of embryo development around the anterior border. At E8.5, expression is found over the anterior neural plate. At E9.5, in the diencephalic part of the ventral forebrain, optic vesicles, olfactory placodes and Rathke pouch. In later stages, present in hypothalamus, eyes and pituitary.
Sequence similarities	Belongs to the SIX/Sine oculis homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Gene Ontology (GO)
Biological process	diencephalon development Inferred from genetic interaction. Source: MGI forebrain anterior/posterior pattern formation Inferred from genetic interaction. Source: MGI lens induction in camera-type eye Inferred from mutant phenotype. Source: MGI negative regulation of Wnt receptor signaling pathway Inferred from mutant phenotype. Source: MGI negative regulation of transcription Inferred from direct assay. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI protein import into nucleus Inferred from direct assay. Source: MGI telencephalon development Inferred from mutant phenotype. Source: MGI
Cellular component	nucleus Ref.2 Inferred from direct assay. Source: MGI
Molecular function	RNA polymerase II transcription factor activity, enhancer binding Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction. Source: MGI sequence-specific DNA binding Ref.2 Inferred from direct assay. Source: MGI transcription repressor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform SIX3B (identifier: Q62233-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform SIX3A (identifier: Q62233-2)

The sequence of this isoform differs from the canonical sequence as follows:
     271-286: LQHQAIGPSGMRSLAE → SVAGTAARPPQA
     287-333: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Homeobox protein SIX3		PRO_0000049300
Regions
	DNA binding	207 – 266	60	Homeobox
	Compositional bias	33 – 70	38	Gly-rich
	Compositional bias	264 – 267	4	Poly-Ala
Natural variations
	Alternative sequence	271 – 286	16	LQHQA…RSLAE → SVAGTAARPPQA in isoform SIX3A.		VSP_002291
	Alternative sequence	287 – 333	47	Missing in isoform SIX3A.		VSP_002292
Experimental info
	Sequence conflict	44	1	G → GG Ref.1
	Sequence conflict	118 – 119	2	VA → WP in CAA62379. Ref.1
	Sequence conflict	278 – 333	56	PSGMR…SECDV → ERDALPGRARLPHARLSRVT VHGGQPDHQCVQPDGARGHR HFDPLGNLQRLGM Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform SIX3B [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 1AD7D3C4388043B9	FASTA	333	35,593
	10 20 30 40 50 60 MVFRSPLDLY SSHFLLPNFA DSHHCSLLLA SSGGGSGASG GGGGAGGGGG GNRAGGGGAG 70 80 90 100 110 120 GAGGGSGGGG SRAPPEELSM FQLPTLNFSP EQVASVCETL EETGDIERLG RFLWSLPVAP 130 140 150 160 170 180 GACEAINKHE SILRARAVVA FHTGNFRDLY HILENHKFTK ESHGKLQAMW LEAHYQEAEK 190 200 210 220 230 240 LRGRPLGPVD KYRVRKKFPL PRTIWDGEQK THCFKERTRS LLREWYLQDP YPNPSKKREL 250 260 270 280 290 300 AQATGLTPTQ VGNWFKNRRQ RDRAAAAKNR LQHQAIGPSG MRSLAEPGCP THGSAESPST 310 320 330 AASPTTSVSS LTERADTGTS ILSVTSSDSE CDV
	Isoform SIX3A. Checksum: A549F11FB9A48237	FASTA	282	30,456
	10 20 30 40 50 60 MVFRSPLDLY SSHFLLPNFA DSHHCSLLLA SSGGGSGASG GGGGAGGGGG GNRAGGGGAG 70 80 90 100 110 120 GAGGGSGGGG SRAPPEELSM FQLPTLNFSP EQVASVCETL EETGDIERLG RFLWSLPVAP 130 140 150 160 170 180 GACEAINKHE SILRARAVVA FHTGNFRDLY HILENHKFTK ESHGKLQAMW LEAHYQEAEK 190 200 210 220 230 240 LRGRPLGPVD KYRVRKKFPL PRTIWDGEQK THCFKERTRS LLREWYLQDP YPNPSKKREL 250 260 270 280 AQATGLTPTQ VGNWFKNRRQ RDRAAAAKNR SVAGTAARPP QA

References

[1]	"Six3, a murine homologue of the sine oculis gene, demarcates the most anterior border of the developing neural plate and is expressed during eye development." Oliver G., Mailhos A., Wehr R., Copeland N.G., Jenkins N.A., Gruss P. Development 121:4045-4055(1995) [PubMed: 8575305] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Embryonic brain.
[2]	"Identification and expression of Six family genes in mouse retina." Kawakami K., Ohto H., Takizawa T., Saito T. FEBS Lett. 393:259-263(1996) [PubMed: 8814301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X90871 mRNA. Translation: CAA62379.1. Different initiation. D83144 mRNA. Translation: BAA11822.1. D83145 mRNA. Translation: BAA11823.1.
IPI	IPI00123350. IPI00230578.
PIR	S74255.
RefSeq	NP_035511.2.
UniGene	Mm.370208 Mm.391586
3D structure databases
HSSP	HSSP built from PDB template 1DU6 based on UniProtKB P41778.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000038805. Mus musculus. [Contig view]
GeneID	20473.
KEGG	mmu:20473.
Organism-specific databases
MGI	MGI:102764. Six3.
Phylogenomic databases
HOVERGEN	Q62233.
Gene expression databases
ArrayExpress	Q62233.
Bgee	Q62233.
GermOnline	ENSMUSG00000038805. Mus musculus.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
ProDom	PD000010. Homeobox. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. False negative. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	298587.
SOURCE	Search...

Entry information

Entry name

SIX3_MOUSE

Accession

Primary (citable) accession number: Q62233
Secondary accession number(s): P70176, P70177

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 15, 1998
Last modified:	March 24, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)