Reviewed, UniProtKB/Swiss-Prot Q62232 (SIX2_MOUSE)

Names and origin

Protein names

Recommended name:
    Homeobox protein SIX2
Alternative name(s):
    Sine oculis homeobox homolog 2

Gene names

Name:

Six2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in limb tendon and ligament development.
Subcellular location	Nucleus.
Tissue specificity	Expressed in phalangeal tendons, in smooth muscle and in head and body mesenchyme.
Developmental stage	First expressed at E8.5 of embryo development in a restricted mesodermal subpopulation at the anterior hindbrain level. Expression of SIX2 in the developing limb begins at E11 and is more pronounced ventrally. It progresses into the developing phalanges at E12.5.
Sequence similarities	Belongs to the SIX/Sine oculis homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Gene Ontology (GO)
Biological process	embryonic skeletal system morphogenesis Inferred from genetic interaction. Source: MGI metanephros development Inferred from mutant phenotype. Source: MGI middle ear morphogenesis Inferred from genetic interaction. Source: MGI negative regulation of cell differentiation Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI protein import into nucleus Inferred from direct assay. Source: MGI
Cellular component	nucleus Ref.2 Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Ref.2 Inferred from direct assay. Source: MGI transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Eya1	P97767	2	EBI-1368736,EBI-1368503

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Homeobox protein SIX2		PRO_0000049298
Regions
	DNA binding	124 – 183	60	Homeobox
Experimental info
	Sequence conflict	1 – 6	6	MSMLPT → HVHARH in CAA56584. Ref.1
	Sequence conflict	105	1	A → R in CAA56584. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q62232-1 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 0FFAB002014D8E60	FASTA	296	32,718
	10 20 30 40 50 60 MSMLPTFGFT QEQVACVCEV LQQGGNIERL GRFLWSLPAC EHLHKNESVL KAKAVVAFHR 70 80 90 100 110 120 GNFRELYKIL ESHQFSPHNH AKLQQLWLKA HYIEAEKLRG RPLGAVGKYR VRRKFPLPRS 130 140 150 160 170 180 IWDGEETSYC FKEKSRSVLR EWYAHNPYPS PREKRELAEA TGLTTTQVSN WFKNRRQRDR 190 200 210 220 230 240 AAEAKERENS ENSNSSSHNP LASSLNGSGK SVLGSSEDEK TPSGTPDHSS SSPALLLSPP 250 260 270 280 290 PPPGLPSLHS LGHPPGPSAV PVPVPGGGGA DPLQHHHSLQ DSILNPMSAN LVDLGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homeobox genes and connective tissue patterning." Oliver G., Wehr R., Jenkins N.A., Copeland N.G., Cheyette B.N.R., Hartenstein V., Zipursky S.L., Gruss P. Development 121:693-705(1995) [PubMed: 7720577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[2]	"Identification and expression of Six family genes in mouse retina." Kawakami K., Ohto H., Takizawa T., Saito T. FEBS Lett. 393:259-263(1996) [PubMed: 8814301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X80338 mRNA. Translation: CAA56584.1. Different initiation. D83147 mRNA. Translation: BAA11825.1. BC068021 mRNA. Translation: AAH68021.1.
IPI	IPI00123346.
PIR	S74253.
RefSeq	NP_035510.1. XP_990400.1.
UniGene	Mm.5039
3D structure databases
HSSP	HSSP built from PDB template 1B72 based on UniProtKB P40424.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62232. 1 interaction.
Genome annotation databases
Ensembl	ENSMUSG00000024134. Mus musculus. [Contig view]
GeneID	20472. 675809.
KEGG	mmu:20472. mmu:675809.
Organism-specific databases
MGI	MGI:102778. Six2.
Phylogenomic databases
HOGENOM	Q62232.
HOVERGEN	Q62232.
OMA	Q62232. HSLGHPQ.
Gene expression databases
ArrayExpress	Q62232.
Bgee	Q62232.
GermOnline	ENSMUSG00000024134. Mus musculus.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
ProDom	PD000010. Homeobox. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	298583.
SOURCE	Search...

Entry information

Entry name

SIX2_MOUSE

Accession

Primary (citable) accession number: Q62232
Secondary accession number(s): P70179

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1998
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)