Q62232 (SIX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein SIX2

Alternative name(s):
Sine oculis homeobox homolog 2

Gene names

Name:

Six2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in limb tendon and ligament development.
Subcellular location	Nucleus.
Tissue specificity	Expressed in phalangeal tendons, in smooth muscle and in head and body mesenchyme.
Developmental stage	First expressed at E8.5 of embryo development in a restricted mesodermal subpopulation at the anterior hindbrain level. Expression of SIX2 in the developing limb begins at E11 and is more pronounced ventrally. It progresses into the developing phalanges at E12.5.
Sequence similarities	Belongs to the SIX/Sine oculis homeobox family. Contains 1 homeobox DNA-binding domain.
Sequence caution	The sequence CAA56584.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Inferred from mutant phenotype PubMed 20515681. Source: MGI chondrocyte differentiation Inferred from mutant phenotype PubMed 20515681. Source: MGI embryonic cranial skeleton morphogenesis Inferred from mutant phenotype PubMed 20515681. Source: MGI mesenchymal to epithelial transition involved in metanephros morphogenesis Inferred from expression pattern PubMed 10322633. Source: UniProtKB mesodermal cell fate specification Inferred from direct assay PubMed 17785448. Source: UniProtKB middle ear morphogenesis Inferred from genetic interaction PubMed 15634706. Source: MGI negative regulation of cell differentiation Inferred from mutant phenotype PubMed 17036046. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 15327782. Source: MGI protein import into nucleus Inferred from direct assay PubMed 16024294. Source: MGI
Cellular_component	nucleus Inferred from direct assay PubMed 15327782Ref.2. Source: MGI
Molecular_function	protein complex binding Inferred from direct assay PubMed 17785448. Source: UniProtKB sequence-specific DNA binding Inferred from direct assay PubMed 15327782Ref.2. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 15327782. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Eya1	P97767	3	EBI-1368736,EBI-1368503

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Homeobox protein SIX2		PRO_0000049298
Regions
	DNA binding	124 – 183	60	Homeobox
Experimental info
	Sequence conflict	1 – 6	6	MSMLPT → HVHARH in CAA56584. Ref.1
	Sequence conflict	105	1	A → R in CAA56584. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q62232 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 0FFAB002014D8E60	FASTA	296	32,718
	10 20 30 40 50 60 MSMLPTFGFT QEQVACVCEV LQQGGNIERL GRFLWSLPAC EHLHKNESVL KAKAVVAFHR 70 80 90 100 110 120 GNFRELYKIL ESHQFSPHNH AKLQQLWLKA HYIEAEKLRG RPLGAVGKYR VRRKFPLPRS 130 140 150 160 170 180 IWDGEETSYC FKEKSRSVLR EWYAHNPYPS PREKRELAEA TGLTTTQVSN WFKNRRQRDR 190 200 210 220 230 240 AAEAKERENS ENSNSSSHNP LASSLNGSGK SVLGSSEDEK TPSGTPDHSS SSPALLLSPP 250 260 270 280 290 PPPGLPSLHS LGHPPGPSAV PVPVPGGGGA DPLQHHHSLQ DSILNPMSAN LVDLGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Homeobox genes and connective tissue patterning." Oliver G., Wehr R., Jenkins N.A., Copeland N.G., Cheyette B.N.R., Hartenstein V., Zipursky S.L., Gruss P. Development 121:693-705(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[2]	"Identification and expression of Six family genes in mouse retina." Kawakami K., Ohto H., Takizawa T., Saito T. FEBS Lett. 393:259-263(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80338 mRNA. Translation: CAA56584.1. Different initiation. D83147 mRNA. Translation: BAA11825.1. BC068021 mRNA. Translation: AAH68021.1.
IPI	IPI00123346.
PIR	S74253.
RefSeq	NP_035510.1. NM_011380.2.
UniGene	Mm.5039.
3D structure databases
ProteinModelPortal	Q62232.
SMR	Q62232. Positions 131-178.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q62232. 1 interaction.
PTM databases
PhosphoSite	Q62232.
Proteomic databases
PRIDE	Q62232.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000024947; ENSMUSP00000024947; ENSMUSG00000024134. ENSMUST00000163568; ENSMUSP00000125871; ENSMUSG00000024134.
GeneID	20472.
KEGG	mmu:20472.
Organism-specific databases
CTD	10736.
MGI	MGI:102778. Six2.
Phylogenomic databases
eggNOG	NOG289502.
GeneTree	ENSGT00540000070251.
HOGENOM	HOG000261680.
HOVERGEN	HBG003609.
InParanoid	Q62232.
OMA	PLSASMN.
OrthoDB	EOG4RJG27.
Gene expression databases
Bgee	Q62232.
Genevestigator	Q62232.
GermOnline	ENSMUSG00000024134. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	298583.
SOURCE	Search...

Entry information

Entry name

SIX2_MOUSE

Accession

Primary (citable) accession number: Q62232
Secondary accession number(s): P70179

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program