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Protein

Nuclear receptor subfamily 0 group B member 2

Gene

Nr0b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 0 group B member 2
Alternative name(s):
Orphan nuclear receptor SHP
Small heterodimer partner
Gene namesi
Name:Nr0b2
Synonyms:Shp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1346344. Nr0b2.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus

  • Note: Colocalizes with NEUROD1 in the nucleus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000537531 – 260Nuclear receptor subfamily 0 group B member 2Add BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57Symmetric dimethylarginine; by PRMT51 Publication1

Post-translational modificationi

Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.1 Publication

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ62227.
PRIDEiQ62227.

PTM databases

iPTMnetiQ62227.
PhosphoSitePlusiQ62227.

Expressioni

Tissue specificityi

Expressed in islets of Langerhans (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000037583.
CleanExiMM_NR0B2.
GenevisibleiQ62227. MM.

Interactioni

Subunit structurei

Heterodimer; efficient DNA binding requires dimerization with another bHLH protein. Interacts (via N-terminus) with NEUROD1 (via N-terminus an C-terminus). Interacts with ID2. Interacts with NR5A2, PPARA and PPARG. May also interact with HNF4A (By similarity). Interacts with RARA, RXRA, THRB, NR5A1, NR1I3 and EID1.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RelaQ042073EBI-4310440,EBI-644400
Sirt1Q923E42EBI-4310440,EBI-1802585
Traf6P701965EBI-4310440,EBI-448028

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204818. 17 interactors.
IntActiQ62227. 3 interactors.
STRINGi10090.ENSMUSP00000039175.

Structurei

Secondary structure

1260
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 25Combined sources6
Helixi57 – 74Combined sources18
Helixi77 – 80Combined sources4
Helixi84 – 92Combined sources9
Helixi95 – 105Combined sources11
Helixi146 – 162Combined sources17
Helixi166 – 177Combined sources12
Beta strandi182 – 184Combined sources3
Helixi188 – 204Combined sources17
Helixi213 – 228Combined sources16
Helixi232 – 239Combined sources8
Helixi241 – 244Combined sources4
Helixi249 – 256Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YMTX-ray1.20B17-30[»]
4NUFX-ray2.80A53-260[»]
ProteinModelPortaliQ62227.
SMRiQ62227.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62227.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 231Ligand-bindingBy similarityAdd BLAST172

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00390000015719.
HOGENOMiHOG000231151.
HOVERGENiHBG008136.
InParanoidiQ62227.
KOiK08563.
OMAiSPKEYAY.
OrthoDBiEOG091G0FGK.
PhylomeDBiQ62227.
TreeFamiTF332386.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiIPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
SMARTiSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62227-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGQSGVCP CQGSAGRPTI LYALLSPSPR TRPVAPASHS HCLCQQQRPV
60 70 80 90 100
RLCAPHRTCR EALDVLAKTV AFLRNLPSFC HLPHEDQRRL LECCWGPLFL
110 120 130 140 150
LGLAQDAVTF EVAEAPVPSI LKKILLEEAS SGTQGAQPSD RPQPSLAAVQ
160 170 180 190 200
WLQRCLESFW SLELGPKEYA YLKGTILFNP DVPGLRASCH IAHLQQEAHW
210 220 230 240 250
ALCEVLEPWY PASQGRLARI LLMASTLKNI PGTLLVDLFF RPIMGDVDIT
260
ELLEDMLLLR
Length:260
Mass (Da):28,763
Last modified:November 1, 1996 - v1
Checksum:i7B859325AA73DCA1
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti241R → C.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76567 Genomic DNA. Translation: AAB59732.1.
AY360323 mRNA. Translation: AAQ55057.1.
AY360324 mRNA. Translation: AAQ55058.1.
BC019540 mRNA. Translation: AAH19540.1.
CCDSiCCDS18751.1.
RefSeqiNP_035980.1. NM_011850.3.
UniGeneiMm.346759.

Genome annotation databases

EnsembliENSMUST00000042706; ENSMUSP00000039175; ENSMUSG00000037583.
GeneIDi23957.
KEGGimmu:23957.
UCSCiuc008vcz.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76567 Genomic DNA. Translation: AAB59732.1.
AY360323 mRNA. Translation: AAQ55057.1.
AY360324 mRNA. Translation: AAQ55058.1.
BC019540 mRNA. Translation: AAH19540.1.
CCDSiCCDS18751.1.
RefSeqiNP_035980.1. NM_011850.3.
UniGeneiMm.346759.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YMTX-ray1.20B17-30[»]
4NUFX-ray2.80A53-260[»]
ProteinModelPortaliQ62227.
SMRiQ62227.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204818. 17 interactors.
IntActiQ62227. 3 interactors.
STRINGi10090.ENSMUSP00000039175.

PTM databases

iPTMnetiQ62227.
PhosphoSitePlusiQ62227.

Proteomic databases

PaxDbiQ62227.
PRIDEiQ62227.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042706; ENSMUSP00000039175; ENSMUSG00000037583.
GeneIDi23957.
KEGGimmu:23957.
UCSCiuc008vcz.1. mouse.

Organism-specific databases

CTDi8431.
MGIiMGI:1346344. Nr0b2.

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00390000015719.
HOGENOMiHOG000231151.
HOVERGENiHBG008136.
InParanoidiQ62227.
KOiK08563.
OMAiSPKEYAY.
OrthoDBiEOG091G0FGK.
PhylomeDBiQ62227.
TreeFamiTF332386.

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.

Miscellaneous databases

EvolutionaryTraceiQ62227.
PROiQ62227.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037583.
CleanExiMM_NR0B2.
GenevisibleiQ62227. MM.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiIPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
SMARTiSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNR0B2_MOUSE
AccessioniPrimary (citable) accession number: Q62227
Secondary accession number(s): Q53Z53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.