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Q62227

- NR0B2_MOUSE

UniProt

Q62227 - NR0B2_MOUSE

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Protein

Nuclear receptor subfamily 0 group B member 2

Gene

Nr0b2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.1 Publication

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: InterPro
  3. steroid hormone receptor activity Source: InterPro
  4. transcription corepressor activity Source: UniProtKB

GO - Biological processi

  1. negative regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
  2. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  3. organ regeneration Source: Ensembl
  4. positive regulation of insulin secretion Source: Ensembl
  5. response to glucose Source: Ensembl
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Receptor, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_234105. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 0 group B member 2
Alternative name(s):
Orphan nuclear receptor SHP
Small heterodimer partner
Gene namesi
Name:Nr0b2
Synonyms:Shp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1346344. Nr0b2.

Subcellular locationi

Cytoplasm By similarity. Nucleus
Note: Colocalizes with NEUROD1 in the nucleus.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
  3. protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Nuclear receptor subfamily 0 group B member 2PRO_0000053753Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571Symmetric dimethylarginine; by PRMT51 Publication

Post-translational modificationi

Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.1 Publication

Keywords - PTMi

Methylation

Proteomic databases

PRIDEiQ62227.

PTM databases

PhosphoSiteiQ62227.

Expressioni

Tissue specificityi

Expressed in islets of Langerhans (at protein level).1 Publication

Gene expression databases

BgeeiQ62227.
CleanExiMM_NR0B2.
GenevestigatoriQ62227.

Interactioni

Subunit structurei

Heterodimer; efficient DNA binding requires dimerization with another bHLH protein. Interacts (via N-terminus) with NEUROD1 (via N-terminus an C-terminus). Interacts with ID2. Interacts with NR5A2, PPARA and PPARG. May also interact with HNF4A (By similarity). Interacts with RARA, RXRA, THRB, NR5A1, NR1I3 and EID1.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RelaQ042073EBI-4310440,EBI-644400
Sirt1Q923E42EBI-4310440,EBI-1802585
Traf6P701965EBI-4310440,EBI-448028

Protein-protein interaction databases

BioGridi204818. 20 interactions.
IntActiQ62227. 3 interactions.
STRINGi10090.ENSMUSP00000039175.

Structurei

Secondary structure

1
260
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 256Combined sources
Helixi57 – 7418Combined sources
Helixi77 – 804Combined sources
Helixi84 – 929Combined sources
Helixi95 – 10511Combined sources
Helixi146 – 16217Combined sources
Helixi166 – 17712Combined sources
Beta strandi182 – 1843Combined sources
Helixi188 – 20417Combined sources
Helixi213 – 22816Combined sources
Helixi232 – 2398Combined sources
Helixi241 – 2444Combined sources
Helixi249 – 2568Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YMTX-ray1.20B17-30[»]
4NUFX-ray2.80A55-260[»]
ProteinModelPortaliQ62227.
SMRiQ62227. Positions 20-260.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62227.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 231172Ligand-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG235713.
GeneTreeiENSGT00390000015719.
HOGENOMiHOG000231151.
HOVERGENiHBG008136.
InParanoidiQ62227.
KOiK08563.
OMAiHIGHLQQ.
OrthoDBiEOG7NSB35.
PhylomeDBiQ62227.
TreeFamiTF332386.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
[Graphical view]
PRINTSiPR00398. STRDHORMONER.
SMARTiSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.

Sequencei

Sequence statusi: Complete.

Q62227-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSGQSGVCP CQGSAGRPTI LYALLSPSPR TRPVAPASHS HCLCQQQRPV
60 70 80 90 100
RLCAPHRTCR EALDVLAKTV AFLRNLPSFC HLPHEDQRRL LECCWGPLFL
110 120 130 140 150
LGLAQDAVTF EVAEAPVPSI LKKILLEEAS SGTQGAQPSD RPQPSLAAVQ
160 170 180 190 200
WLQRCLESFW SLELGPKEYA YLKGTILFNP DVPGLRASCH IAHLQQEAHW
210 220 230 240 250
ALCEVLEPWY PASQGRLARI LLMASTLKNI PGTLLVDLFF RPIMGDVDIT
260
ELLEDMLLLR
Length:260
Mass (Da):28,763
Last modified:November 1, 1996 - v1
Checksum:i7B859325AA73DCA1
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti241 – 2411R → C.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76567 Genomic DNA. Translation: AAB59732.1.
AY360323 mRNA. Translation: AAQ55057.1.
AY360324 mRNA. Translation: AAQ55058.1.
BC019540 mRNA. Translation: AAH19540.1.
CCDSiCCDS18751.1.
RefSeqiNP_035980.1. NM_011850.2.
UniGeneiMm.346759.

Genome annotation databases

EnsembliENSMUST00000042706; ENSMUSP00000039175; ENSMUSG00000037583.
GeneIDi23957.
KEGGimmu:23957.
UCSCiuc008vcz.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76567 Genomic DNA. Translation: AAB59732.1 .
AY360323 mRNA. Translation: AAQ55057.1 .
AY360324 mRNA. Translation: AAQ55058.1 .
BC019540 mRNA. Translation: AAH19540.1 .
CCDSi CCDS18751.1.
RefSeqi NP_035980.1. NM_011850.2.
UniGenei Mm.346759.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1YMT X-ray 1.20 B 17-30 [» ]
4NUF X-ray 2.80 A 55-260 [» ]
ProteinModelPortali Q62227.
SMRi Q62227. Positions 20-260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204818. 20 interactions.
IntActi Q62227. 3 interactions.
STRINGi 10090.ENSMUSP00000039175.

PTM databases

PhosphoSitei Q62227.

Proteomic databases

PRIDEi Q62227.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000042706 ; ENSMUSP00000039175 ; ENSMUSG00000037583 .
GeneIDi 23957.
KEGGi mmu:23957.
UCSCi uc008vcz.1. mouse.

Organism-specific databases

CTDi 8431.
MGIi MGI:1346344. Nr0b2.

Phylogenomic databases

eggNOGi NOG235713.
GeneTreei ENSGT00390000015719.
HOGENOMi HOG000231151.
HOVERGENi HBG008136.
InParanoidi Q62227.
KOi K08563.
OMAi HIGHLQQ.
OrthoDBi EOG7NSB35.
PhylomeDBi Q62227.
TreeFami TF332386.

Enzyme and pathway databases

Reactomei REACT_234105. Nuclear Receptor transcription pathway.

Miscellaneous databases

EvolutionaryTracei Q62227.
NextBioi 303792.
PROi Q62227.
SOURCEi Search...

Gene expression databases

Bgeei Q62227.
CleanExi MM_NR0B2.
Genevestigatori Q62227.

Family and domain databases

Gene3Di 1.10.565.10. 1 hit.
InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
[Graphical view ]
Pfami PF00104. Hormone_recep. 1 hit.
[Graphical view ]
PRINTSi PR00398. STRDHORMONER.
SMARTi SM00430. HOLI. 1 hit.
[Graphical view ]
SUPFAMi SSF48508. SSF48508. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An orphan nuclear hormone receptor that lacks a DNA binding domain and heterodimerizes with other receptors."
    Seol W., Choi H.-S., Moore D.D.
    Science 272:1336-1339(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH RARA; RXRA; THRB; NR5A2 AND NR1I3.
    Tissue: Liver.
  2. "FXR and ABCG5/ABCG8 as determinants of cholesterol gallstone formation from quantitative trait locus mapping in mice."
    Wittenburg H., Lyons M.A., Li R., Churchill G.A., Carey M.C., Paigen B.
    Gastroenterology 125:868-881(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: I/LnJ and PERA/Ei.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  4. "A transcriptional inhibitor targeted by the atypical orphan nuclear receptor SHP."
    Bavner A., Johansson L., Toresson G., Gustafsson J.-A., Treuter E.
    EMBO Rep. 3:478-484(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EID1.
  5. "Orphan nuclear receptor small heterodimer partner, a novel corepressor for a basic helix-loop-helix transcription factor BETA2/neuroD."
    Kim J.Y., Chu K., Kim H.J., Seong H.A., Park K.C., Sanyal S., Takeda J., Ha H., Shong M., Tsai M.J., Choi H.S.
    Mol. Endocrinol. 18:776-790(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Arginine methylation by PRMT5 at a naturally occurring mutation site is critical for liver metabolic regulation by small heterodimer partner."
    Kanamaluru D., Xiao Z., Fang S., Choi S.E., Kim D.H., Veenstra T.D., Kemper J.K.
    Mol. Cell. Biol. 31:1540-1550(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT ARG-57.
  7. Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) OF 17-30 IN COMPLEX WITH NR5A1.

Entry informationi

Entry nameiNR0B2_MOUSE
AccessioniPrimary (citable) accession number: Q62227
Secondary accession number(s): Q53Z53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3