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Q62227

- NR0B2_MOUSE

UniProt

Q62227 - NR0B2_MOUSE

Protein

Nuclear receptor subfamily 0 group B member 2

Gene

Nr0b2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.1 Publication

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding transcription factor activity Source: InterPro
    4. steroid hormone receptor activity Source: InterPro
    5. transcription corepressor activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of sequence-specific DNA binding transcription factor activity Source: UniProtKB
    2. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    3. organ regeneration Source: Ensembl
    4. positive regulation of insulin secretion Source: Ensembl
    5. response to glucose Source: Ensembl
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Receptor, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 0 group B member 2
    Alternative name(s):
    Orphan nuclear receptor SHP
    Small heterodimer partner
    Gene namesi
    Name:Nr0b2
    Synonyms:Shp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1346344. Nr0b2.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus
    Note: Colocalizes with NEUROD1 in the nucleus.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB
    3. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 260260Nuclear receptor subfamily 0 group B member 2PRO_0000053753Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei57 – 571Symmetric dimethylarginine; by PRMT51 Publication

    Post-translational modificationi

    Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.1 Publication

    Keywords - PTMi

    Methylation

    Proteomic databases

    PRIDEiQ62227.

    PTM databases

    PhosphoSiteiQ62227.

    Expressioni

    Tissue specificityi

    Expressed in islets of Langerhans (at protein level).1 Publication

    Gene expression databases

    BgeeiQ62227.
    CleanExiMM_NR0B2.
    GenevestigatoriQ62227.

    Interactioni

    Subunit structurei

    Heterodimer; efficient DNA binding requires dimerization with another bHLH protein. Interacts (via N-terminus) with NEUROD1 (via N-terminus an C-terminus). Interacts with ID2. Interacts with NR5A2, PPARA and PPARG. May also interact with HNF4A By similarity. Interacts with RARA, RXRA, THRB, NR5A1, NR1I3 and EID1.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RelaQ042073EBI-4310440,EBI-644400
    Sirt1Q923E42EBI-4310440,EBI-1802585
    Traf6P701965EBI-4310440,EBI-448028

    Protein-protein interaction databases

    BioGridi204818. 20 interactions.
    IntActiQ62227. 3 interactions.
    STRINGi10090.ENSMUSP00000039175.

    Structurei

    Secondary structure

    1
    260
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi20 – 256
    Helixi57 – 7418
    Helixi77 – 804
    Helixi84 – 929
    Helixi95 – 10511
    Helixi146 – 16217
    Helixi166 – 17712
    Beta strandi182 – 1843
    Helixi188 – 20417
    Helixi213 – 22816
    Helixi232 – 2398
    Helixi241 – 2444
    Helixi249 – 2568

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YMTX-ray1.20B17-30[»]
    4NUFX-ray2.80A55-260[»]
    ProteinModelPortaliQ62227.
    SMRiQ62227. Positions 20-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ62227.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni60 – 231172Ligand-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG235713.
    GeneTreeiENSGT00390000015719.
    HOGENOMiHOG000231151.
    HOVERGENiHBG008136.
    InParanoidiQ62227.
    KOiK08563.
    OMAiHIGHLQQ.
    OrthoDBiEOG7NSB35.
    PhylomeDBiQ62227.
    TreeFamiTF332386.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    InterProiIPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    [Graphical view]
    PRINTSiPR00398. STRDHORMONER.
    SMARTiSM00430. HOLI. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q62227-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSGQSGVCP CQGSAGRPTI LYALLSPSPR TRPVAPASHS HCLCQQQRPV    50
    RLCAPHRTCR EALDVLAKTV AFLRNLPSFC HLPHEDQRRL LECCWGPLFL 100
    LGLAQDAVTF EVAEAPVPSI LKKILLEEAS SGTQGAQPSD RPQPSLAAVQ 150
    WLQRCLESFW SLELGPKEYA YLKGTILFNP DVPGLRASCH IAHLQQEAHW 200
    ALCEVLEPWY PASQGRLARI LLMASTLKNI PGTLLVDLFF RPIMGDVDIT 250
    ELLEDMLLLR 260
    Length:260
    Mass (Da):28,763
    Last modified:November 1, 1996 - v1
    Checksum:i7B859325AA73DCA1
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti241 – 2411R → C.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L76567 Genomic DNA. Translation: AAB59732.1.
    AY360323 mRNA. Translation: AAQ55057.1.
    AY360324 mRNA. Translation: AAQ55058.1.
    BC019540 mRNA. Translation: AAH19540.1.
    CCDSiCCDS18751.1.
    RefSeqiNP_035980.1. NM_011850.2.
    UniGeneiMm.346759.

    Genome annotation databases

    EnsembliENSMUST00000042706; ENSMUSP00000039175; ENSMUSG00000037583.
    GeneIDi23957.
    KEGGimmu:23957.
    UCSCiuc008vcz.1. mouse.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L76567 Genomic DNA. Translation: AAB59732.1 .
    AY360323 mRNA. Translation: AAQ55057.1 .
    AY360324 mRNA. Translation: AAQ55058.1 .
    BC019540 mRNA. Translation: AAH19540.1 .
    CCDSi CCDS18751.1.
    RefSeqi NP_035980.1. NM_011850.2.
    UniGenei Mm.346759.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YMT X-ray 1.20 B 17-30 [» ]
    4NUF X-ray 2.80 A 55-260 [» ]
    ProteinModelPortali Q62227.
    SMRi Q62227. Positions 20-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204818. 20 interactions.
    IntActi Q62227. 3 interactions.
    STRINGi 10090.ENSMUSP00000039175.

    PTM databases

    PhosphoSitei Q62227.

    Proteomic databases

    PRIDEi Q62227.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000042706 ; ENSMUSP00000039175 ; ENSMUSG00000037583 .
    GeneIDi 23957.
    KEGGi mmu:23957.
    UCSCi uc008vcz.1. mouse.

    Organism-specific databases

    CTDi 8431.
    MGIi MGI:1346344. Nr0b2.

    Phylogenomic databases

    eggNOGi NOG235713.
    GeneTreei ENSGT00390000015719.
    HOGENOMi HOG000231151.
    HOVERGENi HBG008136.
    InParanoidi Q62227.
    KOi K08563.
    OMAi HIGHLQQ.
    OrthoDBi EOG7NSB35.
    PhylomeDBi Q62227.
    TreeFami TF332386.

    Miscellaneous databases

    EvolutionaryTracei Q62227.
    NextBioi 303792.
    PROi Q62227.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q62227.
    CleanExi MM_NR0B2.
    Genevestigatori Q62227.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    InterProi IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    [Graphical view ]
    PRINTSi PR00398. STRDHORMONER.
    SMARTi SM00430. HOLI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "An orphan nuclear hormone receptor that lacks a DNA binding domain and heterodimerizes with other receptors."
      Seol W., Choi H.-S., Moore D.D.
      Science 272:1336-1339(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH RARA; RXRA; THRB; NR5A2 AND NR1I3.
      Tissue: Liver.
    2. "FXR and ABCG5/ABCG8 as determinants of cholesterol gallstone formation from quantitative trait locus mapping in mice."
      Wittenburg H., Lyons M.A., Li R., Churchill G.A., Carey M.C., Paigen B.
      Gastroenterology 125:868-881(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: I/LnJ and PERA/Ei.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    4. "A transcriptional inhibitor targeted by the atypical orphan nuclear receptor SHP."
      Bavner A., Johansson L., Toresson G., Gustafsson J.-A., Treuter E.
      EMBO Rep. 3:478-484(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EID1.
    5. "Orphan nuclear receptor small heterodimer partner, a novel corepressor for a basic helix-loop-helix transcription factor BETA2/neuroD."
      Kim J.Y., Chu K., Kim H.J., Seong H.A., Park K.C., Sanyal S., Takeda J., Ha H., Shong M., Tsai M.J., Choi H.S.
      Mol. Endocrinol. 18:776-790(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Arginine methylation by PRMT5 at a naturally occurring mutation site is critical for liver metabolic regulation by small heterodimer partner."
      Kanamaluru D., Xiao Z., Fang S., Choi S.E., Kim D.H., Veenstra T.D., Kemper J.K.
      Mol. Cell. Biol. 31:1540-1550(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT ARG-57.
    7. Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) OF 17-30 IN COMPLEX WITH NR5A1.

    Entry informationi

    Entry nameiNR0B2_MOUSE
    AccessioniPrimary (citable) accession number: Q62227
    Secondary accession number(s): Q53Z53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3