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Q62227 (NR0B2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear receptor subfamily 0 group B member 2
Alternative name(s):
Orphan nuclear receptor SHP
Small heterodimer partner
Gene names
Name:Nr0b2
Synonyms:Shp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1. Ref.1

Subunit structure

Heterodimer; efficient DNA binding requires dimerization with another bHLH protein. Interacts (via N-terminus) with NEUROD1 (via N-terminus an C-terminus). Interacts with ID2. Interacts with NR5A2, PPARA and PPARG. May also interact with HNF4A By similarity. Interacts with RARA, RXRA, THRB, NR5A1, NR1I3 and EID1. Ref.1 Ref.4

Subcellular location

Cytoplasm By similarity. Nucleus. Note: Colocalizes with NEUROD1 in the nucleus By similarity.

Tissue specificity

Expressed in islets of Langerhans (at protein level). Ref.5

Post-translational modification

Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR0 subfamily.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityPolymorphism
   Molecular functionReceptor
Repressor
   PTMMethylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of sequence-specific DNA binding transcription factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

organ regeneration

Inferred from electronic annotation. Source: Ensembl

positive regulation of insulin secretion

Inferred from electronic annotation. Source: Ensembl

response to glucose

Inferred from electronic annotation. Source: Ensembl

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

protein complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction PubMed 20375098PubMed 21725320. Source: IntAct

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

transcription corepressor activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 260260Nuclear receptor subfamily 0 group B member 2
PRO_0000053753

Regions

Region60 – 231172Ligand-binding By similarity

Amino acid modifications

Modified residue571Symmetric dimethylarginine; by PRMT5 Ref.6

Natural variations

Natural variant2411R → C.

Secondary structure

........................... 260
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q62227 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7B859325AA73DCA1

FASTA26028,763
        10         20         30         40         50         60 
MSSGQSGVCP CQGSAGRPTI LYALLSPSPR TRPVAPASHS HCLCQQQRPV RLCAPHRTCR 

        70         80         90        100        110        120 
EALDVLAKTV AFLRNLPSFC HLPHEDQRRL LECCWGPLFL LGLAQDAVTF EVAEAPVPSI 

       130        140        150        160        170        180 
LKKILLEEAS SGTQGAQPSD RPQPSLAAVQ WLQRCLESFW SLELGPKEYA YLKGTILFNP 

       190        200        210        220        230        240 
DVPGLRASCH IAHLQQEAHW ALCEVLEPWY PASQGRLARI LLMASTLKNI PGTLLVDLFF 

       250        260 
RPIMGDVDIT ELLEDMLLLR 

« Hide

References

« Hide 'large scale' references
[1]"An orphan nuclear hormone receptor that lacks a DNA binding domain and heterodimerizes with other receptors."
Seol W., Choi H.-S., Moore D.D.
Science 272:1336-1339(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH RARA; RXRA; THRB; NR5A2 AND NR1I3.
Tissue: Liver.
[2]"FXR and ABCG5/ABCG8 as determinants of cholesterol gallstone formation from quantitative trait locus mapping in mice."
Wittenburg H., Lyons M.A., Li R., Churchill G.A., Carey M.C., Paigen B.
Gastroenterology 125:868-881(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: I/LnJ and PERA/Ei.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
[4]"A transcriptional inhibitor targeted by the atypical orphan nuclear receptor SHP."
Bavner A., Johansson L., Toresson G., Gustafsson J.-A., Treuter E.
EMBO Rep. 3:478-484(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EID1.
[5]"Orphan nuclear receptor small heterodimer partner, a novel corepressor for a basic helix-loop-helix transcription factor BETA2/neuroD."
Kim J.Y., Chu K., Kim H.J., Seong H.A., Park K.C., Sanyal S., Takeda J., Ha H., Shong M., Tsai M.J., Choi H.S.
Mol. Endocrinol. 18:776-790(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Arginine methylation by PRMT5 at a naturally occurring mutation site is critical for liver metabolic regulation by small heterodimer partner."
Kanamaluru D., Xiao Z., Fang S., Choi S.E., Kim D.H., Veenstra T.D., Kemper J.K.
Mol. Cell. Biol. 31:1540-1550(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT ARG-57.
[7]"Structural analyses reveal phosphatidyl inositols as ligands for the NR5 orphan receptors SF-1 and LRH-1."
Krylova I.N., Sablin E.P., Moore J., Xu R.X., Waitt G.M., MacKay J.A., Juzumiene D., Bynum J.M., Madauss K., Montana V., Lebedeva L., Suzawa M., Williams J.D., Williams S.P., Guy R.K., Thornton J.W., Fletterick R.J., Willson T.M., Ingraham H.A.
Cell 120:343-355(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS) OF 17-30 IN COMPLEX WITH NR5A1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L76567 Genomic DNA. Translation: AAB59732.1.
AY360323 mRNA. Translation: AAQ55057.1.
AY360324 mRNA. Translation: AAQ55058.1.
BC019540 mRNA. Translation: AAH19540.1.
CCDSCCDS18751.1.
RefSeqNP_035980.1. NM_011850.2.
UniGeneMm.346759.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YMTX-ray1.20B17-30[»]
4NUFX-ray2.80A55-260[»]
ProteinModelPortalQ62227.
SMRQ62227. Positions 20-260.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204818. 20 interactions.
IntActQ62227. 3 interactions.
STRING10090.ENSMUSP00000039175.

PTM databases

PhosphoSiteQ62227.

Proteomic databases

PRIDEQ62227.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000042706; ENSMUSP00000039175; ENSMUSG00000037583.
GeneID23957.
KEGGmmu:23957.
UCSCuc008vcz.1. mouse.

Organism-specific databases

CTD8431.
MGIMGI:1346344. Nr0b2.

Phylogenomic databases

eggNOGNOG235713.
GeneTreeENSGT00390000015719.
HOGENOMHOG000231151.
HOVERGENHBG008136.
InParanoidQ62227.
KOK08563.
OMAHIGHLQQ.
OrthoDBEOG7NSB35.
PhylomeDBQ62227.
TreeFamTF332386.

Gene expression databases

BgeeQ62227.
CleanExMM_NR0B2.
GenevestigatorQ62227.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
InterProIPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
[Graphical view]
PRINTSPR00398. STRDHORMONER.
SMARTSM00430. HOLI. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ62227.
NextBio303792.
PROQ62227.
SOURCESearch...

Entry information

Entry nameNR0B2_MOUSE
AccessionPrimary (citable) accession number: Q62227
Secondary accession number(s): Q53Z53
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot