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Q62226

- SHH_MOUSE

UniProt

Q62226 - SHH_MOUSE

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Protein

Sonic hedgehog protein

Gene

Shh

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi90 – 901Calcium 13 Publications
Metal bindingi91 – 911Calcium 13 Publications
Metal bindingi91 – 911Calcium 23 Publications
Metal bindingi96 – 961Calcium 13 Publications
Metal bindingi126 – 1261Calcium 1; via carbonyl oxygen3 Publications
Metal bindingi127 – 1271Calcium 13 Publications
Metal bindingi127 – 1271Calcium 23 Publications
Metal bindingi130 – 1301Calcium 23 Publications
Metal bindingi132 – 1321Calcium 23 Publications
Metal bindingi141 – 1411Zinc
Metal bindingi148 – 1481Zinc
Metal bindingi183 – 1831Zinc
Sitei198 – 1992Cleavage; by autolysisBy similarity
Sitei244 – 2441Involved in cholesterol transferBy similarity
Sitei268 – 2681Involved in auto-cleavageBy similarity
Sitei271 – 2711Essential for auto-cleavageBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: UniProtKB
  2. glycoprotein binding Source: MGI
  3. glycosaminoglycan binding Source: MGI
  4. laminin-1 binding Source: MGI
  5. patched binding Source: BHF-UCL
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. anatomical structure development Source: MGI
  2. anatomical structure formation involved in morphogenesis Source: MGI
  3. androgen metabolic process Source: MGI
  4. angiogenesis Source: MGI
  5. anterior/posterior pattern specification Source: MGI
  6. apoptotic process Source: MGI
  7. apoptotic signaling pathway Source: Roslin
  8. artery development Source: MGI
  9. axon guidance Source: MGI
  10. Bergmann glial cell differentiation Source: MGI
  11. blood coagulation Source: MGI
  12. branching involved in prostate gland morphogenesis Source: MGI
  13. branching involved in salivary gland morphogenesis Source: MGI
  14. branching involved in ureteric bud morphogenesis Source: MGI
  15. branching morphogenesis of an epithelial tube Source: MGI
  16. bud outgrowth involved in lung branching Source: MGI
  17. camera-type eye development Source: MGI
  18. canonical Wnt signaling pathway Source: MGI
  19. CD4-positive or CD8-positive, alpha-beta T cell lineage commitment Source: BHF-UCL
  20. cell-cell signaling Source: MGI
  21. cell development Source: MGI
  22. cell fate commitment Source: MGI
  23. cell fate specification Source: MGI
  24. cell proliferation Source: MGI
  25. cellular response to lithium ion Source: MGI
  26. central nervous system development Source: MGI
  27. cerebellar granule cell precursor proliferation Source: UniProtKB
  28. determination of left/right asymmetry in lateral mesoderm Source: BHF-UCL
  29. determination of left/right symmetry Source: MGI
  30. developmental growth Source: MGI
  31. digestive tract morphogenesis Source: MGI
  32. dorsal/ventral neural tube patterning Source: BHF-UCL
  33. dorsal/ventral pattern formation Source: MGI
  34. ectoderm development Source: MGI
  35. embryonic digestive tract morphogenesis Source: BHF-UCL
  36. embryonic digit morphogenesis Source: Roslin
  37. embryonic foregut morphogenesis Source: MGI
  38. embryonic forelimb morphogenesis Source: MGI
  39. embryonic hindlimb morphogenesis Source: MGI
  40. embryonic limb morphogenesis Source: MGI
  41. embryonic morphogenesis Source: MGI
  42. embryonic organ development Source: MGI
  43. embryonic skeletal system development Source: MGI
  44. endocytosis Source: MGI
  45. epithelial cell proliferation involved in salivary gland morphogenesis Source: MGI
  46. epithelial-mesenchymal cell signaling Source: MGI
  47. epithelial-mesenchymal signaling involved in prostate gland development Source: MGI
  48. epithelial tube branching involved in lung morphogenesis Source: MGI
  49. establishment of cell polarity Source: MGI
  50. forebrain development Source: MGI
  51. formation of anatomical boundary Source: MGI
  52. hair follicle development Source: MGI
  53. hair follicle morphogenesis Source: MGI
  54. heart development Source: MGI
  55. heart looping Source: BHF-UCL
  56. hindbrain development Source: MGI
  57. hindgut morphogenesis Source: MGI
  58. inner ear development Source: MGI
  59. intein-mediated protein splicing Source: InterPro
  60. intermediate filament organization Source: MGI
  61. kidney development Source: MGI
  62. left lung development Source: MGI
  63. limb bud formation Source: MGI
  64. limb development Source: MGI
  65. lung-associated mesenchyme development Source: MGI
  66. lung development Source: MGI
  67. lung epithelium development Source: MGI
  68. lung lobe morphogenesis Source: MGI
  69. lung morphogenesis Source: MGI
  70. lymphoid progenitor cell differentiation Source: BHF-UCL
  71. male genitalia development Source: MGI
  72. mesenchymal cell proliferation Source: MGI
  73. mesenchymal cell proliferation involved in lung development Source: MGI
  74. mesenchymal smoothened signaling pathway involved in prostate gland development Source: MGI
  75. metanephric mesenchymal cell proliferation involved in metanephros development Source: UniProtKB
  76. metanephros development Source: UniProtKB
  77. midbrain development Source: MGI
  78. multicellular structure septum development Source: MGI
  79. myoblast differentiation Source: MGI
  80. myotube differentiation Source: MGI
  81. negative regulation of alpha-beta T cell differentiation Source: MGI
  82. negative regulation of apoptotic process Source: Roslin
  83. negative regulation of canonical Wnt signaling pathway Source: MGI
  84. negative regulation of cell differentiation Source: MGI
  85. negative regulation of cell migration Source: MGI
  86. negative regulation of cholesterol efflux Source: BHF-UCL
  87. negative regulation of gene expression Source: UniProtKB
  88. negative regulation of kidney smooth muscle cell differentiation Source: UniProtKB
  89. negative regulation of mesenchymal cell apoptotic process Source: MGI
  90. negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: MGI
  91. negative regulation of protein catabolic process Source: MGI
  92. negative regulation of T cell proliferation Source: MGI
  93. negative regulation of transcription elongation from RNA polymerase II promoter Source: MGI
  94. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  95. negative regulation of ureter smooth muscle cell differentiation Source: UniProtKB
  96. negative regulation of Wnt signaling pathway Source: MGI
  97. negative thymic T cell selection Source: BHF-UCL
  98. neural crest cell migration Source: MGI
  99. neuroblast proliferation Source: MGI
  100. neuron fate commitment Source: MGI
  101. odontogenesis Source: MGI
  102. odontogenesis of dentin-containing tooth Source: MGI
  103. oligodendrocyte development Source: MGI
  104. oligodendrocyte differentiation Source: MGI
  105. organ formation Source: MGI
  106. osteoblast development Source: MGI
  107. palate development Source: MGI
  108. pancreas development Source: MGI
  109. patterning of blood vessels Source: MGI
  110. pattern specification process Source: MGI
  111. polarity specification of anterior/posterior axis Source: Roslin
  112. positive regulation of alpha-beta T cell differentiation Source: BHF-UCL
  113. positive regulation of cell differentiation Source: MGI
  114. positive regulation of cell division Source: Ensembl
  115. positive regulation of cell proliferation Source: MGI
  116. positive regulation of cerebellar granule cell precursor proliferation Source: MGI
  117. positive regulation of epithelial cell proliferation involved in prostate gland development Source: MGI
  118. positive regulation of gene expression Source: UniProtKB
  119. positive regulation of hh target transcription factor activity Source: BHF-UCL
  120. positive regulation of immature T cell proliferation in thymus Source: BHF-UCL
  121. positive regulation of kidney smooth muscle cell differentiation Source: UniProtKB
  122. positive regulation of mesenchymal cell proliferation Source: MGI
  123. positive regulation of mesenchymal cell proliferation involved in ureter development Source: UniProtKB
  124. positive regulation of neuroblast proliferation Source: MGI
  125. positive regulation of oligodendrocyte differentiation Source: MGI
  126. positive regulation of protein import into nucleus Source: MGI
  127. positive regulation of sclerotome development Source: Ensembl
  128. positive regulation of skeletal muscle cell proliferation Source: MGI
  129. positive regulation of skeletal muscle tissue development Source: MGI
  130. positive regulation of smoothened signaling pathway Source: UniProtKB
  131. positive regulation of striated muscle cell differentiation Source: MGI
  132. positive regulation of T cell differentiation in thymus Source: BHF-UCL
  133. positive regulation of transcription, DNA-templated Source: UniProtKB
  134. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  135. positive regulation of ureter smooth muscle cell differentiation Source: UniProtKB
  136. positive regulation of Wnt signaling pathway Source: MGI
  137. positive thymic T cell selection Source: BHF-UCL
  138. primary prostatic bud elongation Source: MGI
  139. prostate epithelial cord elongation Source: MGI
  140. prostate gland development Source: MGI
  141. protein localization to nucleus Source: MGI
  142. proteolysis Source: UniProtKB-KW
  143. regulation of cell proliferation Source: MGI
  144. regulation of epithelial cell proliferation involved in prostate gland development Source: MGI
  145. regulation of gene expression Source: MGI
  146. regulation of mesenchymal cell proliferation involved in prostate gland development Source: MGI
  147. regulation of odontogenesis Source: BHF-UCL
  148. regulation of prostatic bud formation Source: MGI
  149. regulation of proteolysis Source: MGI
  150. regulation of transcription, DNA-templated Source: MGI
  151. respiratory tube development Source: MGI
  152. right lung development Source: MGI
  153. salivary gland cavitation Source: MGI
  154. signal transduction Source: MGI
  155. skin development Source: MGI
  156. smoothened signaling pathway Source: MGI
  157. smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation Source: MGI
  158. somite development Source: BHF-UCL
  159. spinal cord dorsal/ventral patterning Source: MGI
  160. spinal cord motor neuron differentiation Source: MGI
  161. stem cell development Source: BHF-UCL
  162. striated muscle cell differentiation Source: MGI
  163. striated muscle tissue development Source: MGI
  164. T cell differentiation in thymus Source: BHF-UCL
  165. telencephalon regionalization Source: MGI
  166. thalamus development Source: MGI
  167. thymus development Source: BHF-UCL
  168. thyroid gland development Source: MGI
  169. trachea development Source: MGI
  170. trachea morphogenesis Source: MGI
  171. vasculature development Source: MGI
  172. vasculogenesis Source: MGI
  173. vasculogenesis involved in coronary vascular morphogenesis Source: DFLAT
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiC46.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Sonic hedgehog protein
Short name:
SHH
Alternative name(s):
HHG-1
Cleaved into the following 2 chains:
Alternative name(s):
Sonic hedgehog protein 19 kDa product
Alternative name(s):
Sonic hedgehog protein 27 kDa product
Gene namesi
Name:Shh
Synonyms:Hhg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:98297. Shh.

Subcellular locationi

Chain Sonic hedgehog protein C-product : Secretedextracellular space
Note: The C-terminal peptide diffuses from the cell.
Chain Sonic hedgehog protein N-product : Cell membrane; Lipid-anchor
Note: The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside.

GO - Cellular componenti

  1. cell surface Source: MGI
  2. extracellular matrix Source: MGI
  3. extracellular space Source: MGI
  4. membrane Source: MGI
  5. membrane raft Source: MGI
  6. nucleus Source: MGI
  7. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi25 – 251C → S: Strongly reduces effects of in vivo overexpression; impairs multimer formation; does not affect subcellular location to lipid rafts. Homozygous mice are characterized by a smaller size and holoprosencephaly at E10.5, and shortening of limbs at E13.5. They die soon after birth. 2 Publications
Mutagenesisi32 – 321G → R: Introduces a cleavage site for a furin-like protease resulting in abnormal protein processing; cleavage at this site removes 11 amino acids from the N-terminal domain and reduces affinity of Shh for Ptch1 and signaling potency in assays using chicken embryo neural plate explants and mouse C3H10T1/2 stem cells. 1 Publication
Mutagenesisi89 – 891D → V: Moderately reduces Ptch1 binding in vitro and signaling potency in chicken embryo neural plate explant assays compared with wild-type sequence. 1 Publication
Mutagenesisi101 – 1011Q → H: Does not affect signaling activity in any of Shh signaling assays and causes no apparent defects in cholesterol-mediated autoprocessing reactions. 1 Publication
Mutagenesisi116 – 1161N → K: Shows no change in activities at different temperatures. 1 Publication
Mutagenesisi118 – 1181W → G: Causes a failure of Shh processing leading to retention of the immature glycosylated protein within the endoplasmic reticulum of transfected cells; causes a temperature-dependent conformational change that allows Shh to bind Ptch1 at 4 or 32 degrees Celsius but not at 37 degrees Celsius; drastically reduces signaling potency in chicken embryo neural plate explant assays. 1 Publication
Mutagenesisi118 – 1181W → R: Causes a failure of Shh processing leading to retention of the immature glycosylated protein within the endoplasmic reticulum of transfected cells; causes a temperature-dependent conformational change that allows Shh to bind Ptch1 at 4 or 32 degrees Celsius but not at 37 degrees Celsius; drastically reduces signaling potency in chicken embryo neural plate explant assays. 1 Publication
Mutagenesisi189 – 1891E → Q: Does not affect signaling activity in any of Shh signaling assays and causes no apparent defects in cholesterol-mediated autoprocessing reactions. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 437413Sonic hedgehog proteinPRO_0000013211Add
BLAST
Chaini25 – 198174Sonic hedgehog protein N-productPRO_0000013212Add
BLAST
Chaini199 – 437239Sonic hedgehog protein C-productPRO_0000013213Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi25 – 251N-palmitoyl cysteine2 Publications
Lipidationi198 – 1981Cholesterol glycine ester1 Publication
Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.3 Publications
Cholesterylation is required for N-product targeting to lipid rafts and multimerization.1 Publication
N-palmitoylation of Cys-25 by HHAT is required for N-product multimerization and full activity.2 Publications

Keywords - PTMi

Autocatalytic cleavage, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiQ62226.

PTM databases

PhosphoSiteiQ62226.

Expressioni

Tissue specificityi

Expressed in a number of embryonic tissues including the notochord, ventral neural tube, floor plate, lung bud, zone of polarizing activity and posterior distal mesenchyme of limbs. In the adult, expressed in lung and neural retina.

Developmental stagei

First detectable during gastrulation.

Inductioni

By retinoic acid.

Gene expression databases

BgeeiQ62226.
CleanExiMM_SHH.
GenevestigatoriQ62226.

Interactioni

Subunit structurei

Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. N-product is active as a multimer. Interacts with BOC and CDON. Interacts with HHIP.6 Publications

Protein-protein interaction databases

BioGridi203220. 10 interactions.
DIPiDIP-48537N.
STRINGi10090.ENSMUSP00000002708.

Structurei

Secondary structure

1
437
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi48 – 525
Turni57 – 604
Beta strandi69 – 713
Helixi72 – 765
Beta strandi85 – 873
Beta strandi92 – 943
Helixi95 – 973
Helixi101 – 11717
Beta strandi123 – 1275
Beta strandi131 – 1355
Helixi140 – 1434
Beta strandi146 – 1516
Helixi156 – 1583
Helixi159 – 16810
Beta strandi172 – 1787
Beta strandi181 – 1855
Helixi189 – 1924

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VHHX-ray1.70A34-195[»]
2WFXX-ray3.20A40-191[»]
2WG4X-ray3.15A40-191[»]
3D1MX-ray1.70A/B26-189[»]
3N1RX-ray2.13A40-195[»]
4C4MX-ray1.74A40-195[»]
4C4NX-ray2.36A/B40-195[»]
ProteinModelPortaliQ62226.
SMRiQ62226. Positions 39-195, 199-351.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ62226.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi383 – 3875Poly-Gly

Domaini

The sonic hedgehog protein N-product binds calcium and zinc ions; this stabilizes the protein fold and is essential for protein-protein interactions mediated by this domain.3 Publications

Sequence similaritiesi

Belongs to the hedgehog family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG250647.
GeneTreeiENSGT00390000001117.
HOGENOMiHOG000233428.
HOVERGENiHBG005480.
InParanoidiQ62226.
KOiK11988.
OMAiHSWAHRA.
OrthoDBiEOG779NZ5.
PhylomeDBiQ62226.
TreeFamiTF106458.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.30.1380.10. 1 hit.
InterProiIPR001657. Hedgehog.
IPR028992. Hedgehog/Intein_dom.
IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom.
IPR000320. Hedgehog_signalling_dom.
IPR001767. Hint_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
[Graphical view]
PfamiPF01085. HH_signal. 1 hit.
PF01079. Hint. 1 hit.
[Graphical view]
PIRSFiPIRSF009400. Peptidase_C46. 1 hit.
PRINTSiPR00632. SONICHHOG.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 1 hit.
SSF55166. SSF55166. 1 hit.
PROSITEiPS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q62226-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLLLARCFL VILASSLLVC PGLACGPGRG FGKRRHPKKL TPLAYKQFIP
60 70 80 90 100
NVAEKTLGAS GRYEGKITRN SERFKELTPN YNPDIIFKDE ENTGADRLMT
110 120 130 140 150
QRCKDKLNAL AISVMNQWPG VKLRVTEGWD EDGHHSEESL HYEGRAVDIT
160 170 180 190 200
TSDRDRSKYG MLARLAVEAG FDWVYYESKA HIHCSVKAEN SVAAKSGGCF
210 220 230 240 250
PGSATVHLEQ GGTKLVKDLR PGDRVLAADD QGRLLYSDFL TFLDRDEGAK
260 270 280 290 300
KVFYVIETLE PRERLLLTAA HLLFVAPHND SGPTPGPSAL FASRVRPGQR
310 320 330 340 350
VYVVAERGGD RRLLPAAVHS VTLREEEAGA YAPLTAHGTI LINRVLASCY
360 370 380 390 400
AVIEEHSWAH RAFAPFRLAH ALLAALAPAR TDGGGGGSIP AAQSATEARG
410 420 430
AEPTAGIHWY SQLLYHIGTW LLDSETMHPL GMAVKSS
Length:437
Mass (Da):47,773
Last modified:June 1, 1998 - v2
Checksum:iD0EB72F08E7860EF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76290 mRNA. Translation: CAA53922.1.
AK077688 mRNA. Translation: BAC36956.1.
BC063087 mRNA. Translation: AAH63087.1.
CCDSiCCDS19146.1.
PIRiA49425.
RefSeqiNP_033196.1. NM_009170.3.
UniGeneiMm.57202.

Genome annotation databases

EnsembliENSMUST00000002708; ENSMUSP00000002708; ENSMUSG00000002633.
GeneIDi20423.
KEGGimmu:20423.
UCSCiuc008wua.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76290 mRNA. Translation: CAA53922.1 .
AK077688 mRNA. Translation: BAC36956.1 .
BC063087 mRNA. Translation: AAH63087.1 .
CCDSi CCDS19146.1.
PIRi A49425.
RefSeqi NP_033196.1. NM_009170.3.
UniGenei Mm.57202.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VHH X-ray 1.70 A 34-195 [» ]
2WFX X-ray 3.20 A 40-191 [» ]
2WG4 X-ray 3.15 A 40-191 [» ]
3D1M X-ray 1.70 A/B 26-189 [» ]
3N1R X-ray 2.13 A 40-195 [» ]
4C4M X-ray 1.74 A 40-195 [» ]
4C4N X-ray 2.36 A/B 40-195 [» ]
ProteinModelPortali Q62226.
SMRi Q62226. Positions 39-195, 199-351.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203220. 10 interactions.
DIPi DIP-48537N.
STRINGi 10090.ENSMUSP00000002708.

Chemistry

BindingDBi Q62226.
ChEMBLi CHEMBL5387.

Protein family/group databases

MEROPSi C46.002.

PTM databases

PhosphoSitei Q62226.

Proteomic databases

PRIDEi Q62226.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000002708 ; ENSMUSP00000002708 ; ENSMUSG00000002633 .
GeneIDi 20423.
KEGGi mmu:20423.
UCSCi uc008wua.2. mouse.

Organism-specific databases

CTDi 6469.
MGIi MGI:98297. Shh.

Phylogenomic databases

eggNOGi NOG250647.
GeneTreei ENSGT00390000001117.
HOGENOMi HOG000233428.
HOVERGENi HBG005480.
InParanoidi Q62226.
KOi K11988.
OMAi HSWAHRA.
OrthoDBi EOG779NZ5.
PhylomeDBi Q62226.
TreeFami TF106458.

Miscellaneous databases

EvolutionaryTracei Q62226.
NextBioi 298432.
PROi Q62226.
SOURCEi Search...

Gene expression databases

Bgeei Q62226.
CleanExi MM_SHH.
Genevestigatori Q62226.

Family and domain databases

Gene3Di 2.170.16.10. 1 hit.
3.30.1380.10. 1 hit.
InterProi IPR001657. Hedgehog.
IPR028992. Hedgehog/Intein_dom.
IPR009045. Hedgehog_sig/DD-Pept_Zn-bd_dom.
IPR000320. Hedgehog_signalling_dom.
IPR001767. Hint_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR006141. Intein_splice_site.
[Graphical view ]
Pfami PF01085. HH_signal. 1 hit.
PF01079. Hint. 1 hit.
[Graphical view ]
PIRSFi PIRSF009400. Peptidase_C46. 1 hit.
PRINTSi PR00632. SONICHHOG.
SMARTi SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 1 hit.
SSF55166. SSF55166. 1 hit.
PROSITEi PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity."
    Echelard Y., Epstein D.J., St Jacques B., Shen L., Mohler J., McMahon J.A., McMahon A.P.
    Cell 75:1417-1430(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. McMahon A.P.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 122.
  3. "Products, genetic linkage and limb patterning activity of a murine hedgehog gene."
    Chang D.T., Lopez A., von Kessler D.P., Chiang C., Simandl B.K., Zhao R., Seldin M.F., Fallon J.F., Beachy P.A.
    Development 120:3339-3353(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEOLYTIC PROCESSING, AUTOCATALYTIC CLEAVAGE.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Limb.
  6. "Proteolytic processing yields two secreted forms of sonic hedgehog."
    Bumcrot D.A., Takada R., McMahon A.P.
    Mol. Cell. Biol. 15:2294-2303(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING, GLYCOSYLATION, SUBCELLULAR LOCATION.
  7. "Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis."
    Roelink H., Porter J.A., Chiang C., Tanabe Y., Chang D.T., Beachy P.A., Jessell T.M.
    Cell 81:445-455(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PROTEOLYTIC PROCESSING, AUTOCATALYTIC CLEAVAGE.
  8. "Cholesterol modification of hedgehog signaling proteins in animal development."
    Porter J.A., Young K.E., Beachy P.A.
    Science 274:255-259(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHOLESTERYLATION AT GLY-198.
  9. "Skinny hedgehog, an acyltransferase required for palmitoylation and activity of the hedgehog signal."
    Chamoun Z., Mann R.K., Nellen D., von Kessler D.P., Bellotto M., Beachy P.A., Basler K.
    Science 293:2080-2084(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-25, MUTAGENESIS OF CYS-25.
  10. "RORalpha coordinates reciprocal signaling in cerebellar development through sonic hedgehog and calcium-dependent pathways."
    Gold D.A., Baek S.H., Schork N.J., Rose D.W., Larsen D.D., Sachs B.D., Rosenfeld M.G., Hamilton B.A.
    Neuron 40:1119-1131(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CEREBELLAR DEVELOPMENT.
  11. "Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates."
    Chen M.-H., Li Y.-J., Kawakami T., Xu S.-M., Chuang P.-T.
    Genes Dev. 18:641-659(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-25, MUTAGENESIS OF CYS-25, SUBUNIT, SUBCELLULAR LOCATION.
  12. "Molecular mechanisms of Sonic hedgehog mutant effects in holoprosencephaly."
    Maity T., Fuse N., Beachy P.A.
    Proc. Natl. Acad. Sci. U.S.A. 102:17026-17031(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF GLY-32; ASP-89; GLN-101; ASN-116; TRP-118 AND GLU-189.
  13. "Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog."
    Abe Y., Kita Y., Niikura T.
    FEBS J. 275:318-331(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HHATL.
  14. "A potential catalytic site revealed by the 1.7-A crystal structure of the amino-terminal signalling domain of Sonic hedgehog."
    Hall T.M.T., Porter J.A., Beachy P.A., Leahy D.J.
    Nature 378:212-216(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 34-195 IN COMPLEX WITH ZINC IONS.
  15. "The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla."
    McLellan J.S., Zheng X., Hauk G., Ghirlando R., Beachy P.A., Leahy D.J.
    Nature 455:979-983(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 26-189 IN COMPLEX WITH CDON; CALCIUM AND ZINC IONS, DOMAIN, INTERACTION WITH CDON.
  16. "Structural insights into hedgehog ligand sequestration by the human hedgehog-interacting protein HHIP."
    Bishop B., Aricescu A.R., Harlos K., O'Callaghan C.A., Jones E.Y., Siebold C.
    Nat. Struct. Mol. Biol. 16:698-703(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 40-191 IN COMPLEX WITH HHIP; CALCIUM AND ZINC IONS, DOMAIN, INTERACTION WITH HHIP.
  17. "All mammalian Hedgehog proteins interact with cell adhesion molecule, down-regulated by oncogenes (CDO) and brother of CDO (BOC) in a conserved manner."
    Kavran J.M., Ward M.D., Oladosu O.O., Mulepati S., Leahy D.J.
    J. Biol. Chem. 285:24584-24590(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 26-189 IN COMPLEX WITH CALCIUM AND ZINC IONS, DOMAIN, INTERACTION WITH BOC AND CDON.

Entry informationi

Entry nameiSHH_MOUSE
AccessioniPrimary (citable) accession number: Q62226
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mice overexpressing Shh display digit duplications in both forelimbs and hindlimbs.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3